LIVIVO - Search results -

Search results

Result 1 - 10 of total 23

Search options

Article ; Online: Automated Detection and Localization of Synaptic Vesicles in Electron Microscopy Images.

Imbrosci, Barbara / Schmitz, Dietmar / Orlando, Marta

2022 Volume 9, Issue 1

Abstract: Information transfer and integration in the brain occurs at chemical synapses and is mediated by the fusion of synaptic vesicles filled with neurotransmitter. Synaptic vesicle dynamic spatial organization regulates synaptic transmission as well as ... ...

Abstract	Information transfer and integration in the brain occurs at chemical synapses and is mediated by the fusion of synaptic vesicles filled with neurotransmitter. Synaptic vesicle dynamic spatial organization regulates synaptic transmission as well as synaptic plasticity. Because of their small size, synaptic vesicles require electron microscopy (EM) for their imaging, and their analysis is conducted manually. The manual annotation and segmentation of the hundreds to thousands of synaptic vesicles, is highly time consuming and limits the throughput of data collection. To overcome this limitation, we built an algorithm, mainly relying on convolutional neural networks (CNNs), capable of automatically detecting and localizing synaptic vesicles in electron micrographs. The algorithm was trained on murine synapses but we show that it works well on synapses from different species, ranging from zebrafish to human, and from different preparations. As output, we provide the vesicle count and coordinates, the nearest neighbor distance (nnd) and the estimate of the vesicles area. We also provide a graphical user interface (GUI) to guide users through image analysis, result visualization, and manual proof-reading. The application of our algorithm is especially recommended for images produced by transmission EM. Since this type of imaging is used routinely to investigate presynaptic terminals, our solution will likely be of interest for numerous research groups.
MeSH term(s)	Animals ; Humans ; Mice ; Microscopy, Electron ; Presynaptic Terminals ; Synapses ; Synaptic Vesicles ; Zebrafish
Language	English
Publishing date	2022-01-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2800598-3
ISSN	2373-2822 ; 2373-2822
ISSN (online)	2373-2822
ISSN	2373-2822
DOI	10.1523/ENEURO.0400-20.2021
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Subiculum as a generator of sharp wave-ripples in the rodent hippocampus.

Imbrosci, Barbara / Nitzan, Noam / McKenzie, Sam / Donoso, José R / Swaminathan, Aarti / Böhm, Claudia / Maier, Nikolaus / Schmitz, Dietmar

Cell reports

2021 Volume 35, Issue 3, Page(s) 109021

Abstract: Sharp wave-ripples (SWRs) represent synchronous discharges of hippocampal neurons and are believed to play a major role in memory consolidation. A large body of evidence suggests that SWRs are exclusively generated in the CA3-CA2 network. In contrast, ... ...

Abstract	Sharp wave-ripples (SWRs) represent synchronous discharges of hippocampal neurons and are believed to play a major role in memory consolidation. A large body of evidence suggests that SWRs are exclusively generated in the CA3-CA2 network. In contrast, here, we provide several lines of evidence showing that the subiculum can function as a secondary SWRs generator. SWRs with subicular origin propagate forward into the entorhinal cortex as well as backward into the hippocampus proper. Our findings suggest that the output structures of the hippocampus are not only passively facilitating the transfer of SWRs to the cortex, but they also can actively contribute to the genesis of SWRs. We hypothesize that SWRs with a subicular origin may be important for the consolidation of information conveyed to the hippocampus via the temporoammonic pathway.
MeSH term(s)	Animals ; Brain Waves/physiology ; CA1 Region, Hippocampal/anatomy & histology ; CA1 Region, Hippocampal/physiology ; CA3 Region, Hippocampal/anatomy & histology ; CA3 Region, Hippocampal/physiology ; Electrodes, Implanted ; Entorhinal Cortex/anatomy & histology ; Entorhinal Cortex/physiology ; Male ; Memory Consolidation/physiology ; Mice ; Mice, Inbred C57BL ; Microtomy ; Neurons/cytology ; Neurons/physiology ; Patch-Clamp Techniques ; Rats ; Rats, Long-Evans ; Synaptic Potentials/physiology ; Synaptic Transmission/physiology
Language	English
Publishing date	2021-04-18
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2021.109021
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Neue Befunde zur Funktion der GABAergen Hemmung während post-läsionaler Reorganisation im visuellen Kortex

Imbrosci, Barbara / Mittmann, Thomas

Neuroforum

2014 Volume 20, Issue 1, Page(s) 178

Language	German
Document type	Article
ZDB-ID	1238592-x
ISSN	0947-0875
Database	Current Contents Medicine

In stock of ZB MED Cologne/Königswinter

Zs.A 4805: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Physiological properties of supragranular cortical inhibitory interneurons expressing retrograde persistent firing.

Imbrosci, Barbara / Neitz, Angela / Mittmann, Thomas

Neural plasticity

2015 Volume 2015, Page(s) 608141

Abstract: Neurons are polarized functional units. The somatodendritic compartment receives and integrates synaptic inputs while the axon relays relevant synaptic information in form of action potentials (APs) across long distance. Despite this well accepted notion, ...

Abstract	Neurons are polarized functional units. The somatodendritic compartment receives and integrates synaptic inputs while the axon relays relevant synaptic information in form of action potentials (APs) across long distance. Despite this well accepted notion, recent research has shown that, under certain circumstances, the axon can also generate APs independent of synaptic inputs at axonal sites distal from the soma. These ectopic APs travel both toward synaptic terminals and antidromically toward the soma. This unusual form of neuronal communication seems to preferentially occur in cortical inhibitory interneurons following a period of intense neuronal activity and might have profound implications for neuronal information processing. Here we show that trains of ectopically generated APs can be induced in a large portion of neocortical layer 2/3 GABAergic interneurons following a somatic depolarization inducing hundreds of APs. Sparsely occurring ectopic spikes were also observed in a large portion of layer 1 interneurons even in absence of prior somatic depolarization. Remarkably, we found that interneurons which produce ectopic APs display specific membrane and morphological properties significantly different from the remaining GABAergic cells and may therefore represent a functionally unique interneuronal subpopulation.
MeSH term(s)	Action Potentials ; Animals ; GABAergic Neurons/cytology ; GABAergic Neurons/physiology ; Interneurons/cytology ; Interneurons/physiology ; Mice, Inbred C57BL ; Neocortex/cytology ; Neocortex/physiology ; Synapses/physiology
Language	English
Publishing date	2015-02-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1454938-4
ISSN	1687-5443 ; 2090-5904 ; 0792-8483
ISSN (online)	1687-5443
ISSN	2090-5904 ; 0792-8483
DOI	10.1155/2015/608141
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3305: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Neuronal mechanisms underlying transhemispheric diaschisis following focal cortical injuries.

Imbrosci, Barbara / Wang, Yibao / Arckens, Lutgarde / Mittmann, Thomas

Brain structure & function

2015 Volume 220, Issue 3, Page(s) 1649–1664

Abstract: Unilateral cortical lesions cause disturbances often spreading into the hemisphere contralateral to the injury. The functional alteration affecting the contralesional cortex is called transhemispheric diaschisis and is believed to contribute to ... ...

Abstract	Unilateral cortical lesions cause disturbances often spreading into the hemisphere contralateral to the injury. The functional alteration affecting the contralesional cortex is called transhemispheric diaschisis and is believed to contribute to neurological deficits and to processes of functional reorganization post-lesion. Despite the profound implications for recovery, little is known about the cellular mechanisms that underlie this phenomenon. In the present study, transhemispheric diaschisis was investigated with an in vivo-ex vivo model of unilateral lesions, induced by an infrared laser in rat visual cortex. Visually evoked cortical activity was evaluated by the expression level of the cellular activity marker zif268, which showed an elevation in the cortex contralateral to the lesion. In vitro patch-clamp recordings from layer 2/3 pyramidal neurons revealed a shift in the excitatory–inhibitory balance in favor of excitability, particularly expressed in the undamaged hemisphere. Layer 5 principal neurons displayed an increased spontaneous firing rate contralateral to the lesion, while cells of the injured cortex displayed a reduced firing upon somatic current injection. These data suggest that a cortical lesion triggers an enhanced neuronal activity in the hemisphere contralateral to the damage. Our findings constitute an important step toward the understanding of transhemispheric diaschisis on the cellular level.
MeSH term(s)	Animals ; Brain Injuries/physiopathology ; Cerebral Cortex/injuries ; Cerebral Cortex/metabolism ; Cerebral Cortex/pathology ; Cerebral Cortex/physiopathology ; Early Growth Response Protein 1/metabolism ; Excitatory Postsynaptic Potentials ; Functional Laterality/physiology ; Inhibitory Postsynaptic Potentials ; Neurons/metabolism ; Neurons/physiology ; Photic Stimulation ; RNA, Messenger/metabolism ; Rats ; Rats, Long-Evans ; Synapses/physiology ; Visual Cortex/injuries
Chemical Substances	Early Growth Response Protein 1 ; Egr1 protein, rat ; RNA, Messenger
Language	English
Publishing date	2015
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2273162-3
ISSN	1863-2661 ; 1863-2653
ISSN (online)	1863-2661
ISSN	1863-2653
DOI	10.1007/s00429-014-0750-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Ub I Zs.90: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Subiculum as a generator of sharp wave-ripples in the rodent hippocampus

Barbara Imbrosci / Noam Nitzan / Sam McKenzie / José R. Donoso / Aarti Swaminathan / Claudia Böhm / Nikolaus Maier / Dietmar Schmitz

Cell Reports, Vol 35, Iss 3, Pp 109021- (2021)

2021

Abstract: Summary: Sharp wave-ripples (SWRs) represent synchronous discharges of hippocampal neurons and are believed to play a major role in memory consolidation. A large body of evidence suggests that SWRs are exclusively generated in the CA3-CA2 network. In ... ...

Abstract	Summary: Sharp wave-ripples (SWRs) represent synchronous discharges of hippocampal neurons and are believed to play a major role in memory consolidation. A large body of evidence suggests that SWRs are exclusively generated in the CA3-CA2 network. In contrast, here, we provide several lines of evidence showing that the subiculum can function as a secondary SWRs generator. SWRs with subicular origin propagate forward into the entorhinal cortex as well as backward into the hippocampus proper. Our findings suggest that the output structures of the hippocampus are not only passively facilitating the transfer of SWRs to the cortex, but they also can actively contribute to the genesis of SWRs. We hypothesize that SWRs with a subicular origin may be important for the consolidation of information conveyed to the hippocampus via the temporoammonic pathway.
Keywords	hippocampus ; CA3 ; CA1 ; subiculum ; entorhinal cortex ; oscillations ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2021-04-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Functional consequences of the disturbances in the GABA-mediated inhibition induced by injuries in the cerebral cortex.

Imbrosci, Barbara / Mittmann, Thomas

Neural plasticity

2011 Volume 2011, Page(s) 614329

Abstract: Cortical injuries are often reported to induce a suppression of the intracortical GABAergic inhibition in the surviving, neighbouring neuronal networks. Since GABAergic transmission provides the main source of inhibition in the mammalian brain, this ... ...

Abstract	Cortical injuries are often reported to induce a suppression of the intracortical GABAergic inhibition in the surviving, neighbouring neuronal networks. Since GABAergic transmission provides the main source of inhibition in the mammalian brain, this condition may lead to hyperexcitability and epileptiform activity of cortical networks. However, inhibition plays also a crucial role in limiting the plastic properties of neuronal circuits, and as a consequence, interventions aiming to reestablish a normal level of inhibition might constrain the plastic capacity of the cortical tissue. A promising strategy to minimize the deleterious consequences of a modified inhibitory transmission without preventing the potential beneficial effects on cortical plasticity may be to unravel distinct GABAergic signaling pathways separately mediating these positive and negative events. Here, gathering data from several recent studies, we provide new insights to better face with this "double coin" condition in the attempt to optimize the functional recovery of patients.
MeSH term(s)	Brain Injuries/metabolism ; Brain Injuries/physiopathology ; Cerebral Cortex/injuries ; Cerebral Cortex/metabolism ; Cerebral Cortex/physiopathology ; Humans ; Neural Inhibition/physiology ; Neurons/physiology ; Synaptic Transmission/physiology ; gamma-Aminobutyric Acid/metabolism
Chemical Substances	gamma-Aminobutyric Acid (56-12-2)
Language	English
Publishing date	2011-05-31
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1454938-4
ISSN	1687-5443 ; 2090-5904 ; 0792-8483
ISSN (online)	1687-5443
ISSN	2090-5904 ; 0792-8483
DOI	10.1155/2011/614329
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3305: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Focal cortical lesions induce bidirectional changes in the excitability of fast spiking and non fast spiking cortical interneurons.

Imbrosci, Barbara / Neitz, Angela / Mittmann, Thomas

PloS one

2014 Volume 9, Issue 10, Page(s) e111105

Abstract: A physiological brain function requires neuronal networks to operate within a well-defined range of activity. Indeed, alterations in neuronal excitability have been associated with several pathological conditions, ranging from epilepsy to ... ...

Abstract	A physiological brain function requires neuronal networks to operate within a well-defined range of activity. Indeed, alterations in neuronal excitability have been associated with several pathological conditions, ranging from epilepsy to neuropsychiatric disorders. Changes in inhibitory transmission are known to play a key role in the development of hyperexcitability. However it is largely unknown whether specific interneuronal subpopulations contribute differentially to such pathological condition. In the present study we investigated functional alterations of inhibitory interneurons embedded in a hyperexcitable cortical circuit at the border of chronically induced focal lesions in mouse visual cortex. Interestingly, we found opposite alterations in the excitability of non fast-spiking (Non Fs) and fast-spiking (Fs) interneurons in acute cortical slices from injured animals. Non Fs interneurons displayed a depolarized membrane potential and a higher frequency of spontaneous excitatory postsynaptic currents (sEPSCs). In contrast, Fs interneurons showed a reduced sEPSCs amplitude. The observed downscaling of excitatory synapses targeting Fs interneurons may prevent the recruitment of this specific population of interneurons to the hyperexcitable network. This mechanism is likely to seriously affect neuronal network function and to exacerbate hyperexcitability but it may be important to protect this particular vulnerable population of GABAegic neurons from excitotoxicity.
MeSH term(s)	Action Potentials ; Animals ; Cerebral Cortex/cytology ; Cerebral Cortex/injuries ; Cerebral Cortex/physiology ; Excitatory Postsynaptic Potentials ; Interneurons/physiology ; Mice ; Visual Cortex/cytology ; Visual Cortex/physiology
Language	English
Publishing date	2014-10-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0111105
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Physiological Properties of Supragranular Cortical Inhibitory Interneurons Expressing Retrograde Persistent Firing

Barbara Imbrosci / Angela Neitz / Thomas Mittmann

Neural Plasticity, Vol

2015 Volume 2015

Keywords	Medicine ; R ; Internal medicine ; RC31-1245 ; Neurosciences. Biological psychiatry. Neuropsychiatry ; RC321-571
Publishing date	2015-01-01T00:00:00Z
Publisher	Hindawi Publishing Corporation
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: A Cellular Mechanism Underlying Enhanced Capability for Complex Olfactory Discrimination Learning.

Chandra, Naveen / Awasthi, Richa / Ozdogan, Togba / Johenning, Friedrich W / Imbrosci, Barbara / Morris, Genela / Schmitz, Dietmar / Barkai, Edi

eNeuro

2019 Volume 6, Issue 1

Abstract: The biological mechanisms underlying complex forms of learning requiring the understanding of rules based on previous experience are not yet known. Previous studies have raised the intriguing possibility that improvement in complex learning tasks ... ...

Abstract	The biological mechanisms underlying complex forms of learning requiring the understanding of rules based on previous experience are not yet known. Previous studies have raised the intriguing possibility that improvement in complex learning tasks requires the long-term modulation of intrinsic neuronal excitability, induced by reducing the conductance of the slow calcium-dependent potassium current (sI
MeSH term(s)	Animals ; Discrimination Learning/physiology ; Excitatory Amino Acid Agonists/pharmacology ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Kainic Acid/pharmacology ; Male ; Maze Learning/physiology ; Membrane Potentials/drug effects ; Membrane Potentials/physiology ; Mice, Inbred C57BL ; Mice, Knockout ; Olfactory Perception/physiology ; Piriform Cortex/drug effects ; Piriform Cortex/physiology ; Protein Kinase C/metabolism ; Pyramidal Cells/drug effects ; Pyramidal Cells/physiology ; Rats, Sprague-Dawley ; Receptors, Kainic Acid/genetics ; Receptors, Kainic Acid/metabolism ; Tissue Culture Techniques ; GluK2 Kainate Receptor
Chemical Substances	Excitatory Amino Acid Agonists ; Receptors, Kainic Acid ; Protein Kinase C (EC 2.7.11.13) ; Extracellular Signal-Regulated MAP Kinases (EC 2.7.11.24) ; Kainic Acid (SIV03811UC)
Language	English
Publishing date	2019-02-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2800598-3
ISSN	2373-2822 ; 2373-2822
ISSN (online)	2373-2822
ISSN	2373-2822
DOI	10.1523/ENEURO.0198-18.2019
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito