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  1. Book ; Conference proceedings ; Collection: Mechanics and dynamics of the cytoskeleton

    Gimona, Mario

    selected research articles and reviews from the 2008 European Cytoskeleton Forum / FEBS Workshop Entitled Mechanics and Dynamics of the Cytoskeleton ; June 22 - 26, 2008, Potsdam, Germany

    (Cell motility and the cytoskeleton ; ...)

    2009  

    Institution European Cytoskeleton Forum
    Event/congress Workshop Entitled Mechanics and Dynamics of the Cytoskeleton (2008, Potsdam)
    Author's details guest ed. Mario Gimona
    Series title Cell motility and the cytoskeleton
    ...
    Language English
    Publisher Wiley
    Publishing place Hoboken, NJ
    Publishing country United States
    Document type Book ; Conference proceedings ; Collection (display volumes)
    HBZ-ID HT016094545
    Database Catalogue ZB MED Medicine, Health

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  2. Book ; Conference proceedings: Mechanics and dynamics of the cytoskeleton / 1

    Gimona, Mario

    selected research articles and reviews from the 2008 European Cytoskeleton Forum / FEBS Workshop Entitled Mechanics and Dynamics of the Cytoskeleton ; June 22 - 26, 2008, Potsdam, Germany

    (Cell motility and the cytoskeleton ; 66,10 = Special issue)

    2009  

    Institution European Cytoskeleton Forum
    Event/congress Workshop Entitled Mechanics and Dynamics of the Cytoskeleton (2008, Potsdam)
    Author's details guest ed. Mario Gimona
    Series title Cell motility and the cytoskeleton ; 66,10 = Special issue
    Mechanics and dynamics of the cytoskeleton
    Collection Mechanics and dynamics of the cytoskeleton
    Language English
    Size S. 769 - 908 : Ill., graph. Darst.
    Publisher Wiley
    Publishing place Hoboken, NJ
    Publishing country United States
    Document type Book ; Conference proceedings
    HBZ-ID HT016094554
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 1840 -66- not available for loan Zeitschriften-Lesesaal

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  3. Book ; Conference proceedings: Mechanics and dynamics of the cytoskeleton / 2

    Gimona, Mario

    selected research articles and reviews from the 2008 European Cytoskeleton Forum / FEBS Workshop Entitled Mechanics and Dynamics of the Cytoskeleton ; June 22 - 26, 2008, Potsdam, Germany

    (Cell motility and the cytoskeleton ; 66,11 = Special issue)

    2009  

    Institution European Cytoskeleton Forum
    Event/congress Workshop Entitled Mechanics and Dynamics of the Cytoskeleton (2008, Potsdam)
    Author's details guest ed. Mario Gimona
    Series title Cell motility and the cytoskeleton ; 66,11 = Special issue
    Mechanics and dynamics of the cytoskeleton
    Collection Mechanics and dynamics of the cytoskeleton
    Language English
    Size III S., S. 909 - 1040 : Ill., graph. Darst.
    Publisher Wiley
    Publishing place Hoboken, NJ
    Publishing country United States
    Document type Book ; Conference proceedings
    HBZ-ID HT016145340
    Database Catalogue ZB MED Medicine, Health

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 1840 -66- not available for loan Zeitschriften-Lesesaal

    Order via subito

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  4. Article ; Online: Calorimetry of extracellular vesicles fusion to single phospholipid membrane.

    Grava, Miriam / Helmy, Sally / Gimona, Mario / Parisse, Pietro / Casalis, Loredana / Brocca, Paola / Rondelli, Valeria

    Biomolecular concepts

    2022  Volume 13, Issue 1, Page(s) 148–155

    Abstract: Extracellular vesicles (EVs)-mediated communication relies not only on the delivery of complex molecular cargoes as lipids, proteins, genetic material, and metabolites to their target cells but also on the modification of the cell surface local ... ...

    Abstract Extracellular vesicles (EVs)-mediated communication relies not only on the delivery of complex molecular cargoes as lipids, proteins, genetic material, and metabolites to their target cells but also on the modification of the cell surface local properties induced by the eventual fusion of EVs' membranes with the cells' plasma membrane. Here we applied scanning calorimetry to study the phase transition of single phospholipid (DMPC) monolamellar vesicles, investigating the thermodynamical effects caused by the fusion of doping amounts of mesenchymal stem cells-derived EVs. Specifically, we studied EVs-induced consequences on the lipids distributed in the differently curved membrane leaflets, having different density and order. The effect of EV components was found to be not homogeneous in the two leaflets, the inner (more disordered one) being mainly affected. Fusion resulted in phospholipid membrane flattening associated with lipid ordering, while the transition cooperativity, linked to membrane domains' coexistence during the transition process, was decreased. Our results open new horizons for the investigation of the peculiar effects of EVs of different origins on target cell membrane properties and functionality.
    MeSH term(s) Calorimetry ; Cell Membrane ; Extracellular Vesicles/chemistry ; Extracellular Vesicles/metabolism ; Mesenchymal Stem Cells ; Phospholipids/analysis ; Phospholipids/metabolism
    Chemical Substances Phospholipids
    Language English
    Publishing date 2022-03-21
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2557908-3
    ISSN 1868-503X ; 1868-5021
    ISSN (online) 1868-503X
    ISSN 1868-5021
    DOI 10.1515/bmc-2022-0011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 7056: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Evaluation of the Potential of Umbilical Cord Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles to Improve Rotator Cuff Healing: A Pilot Ovine Study.

    Jenner, Florien / Wagner, Andrea / Gerner, Iris / Ludewig, Eberhard / Trujanovic, Robert / Rohde, Eva / von Rechenberg, Brigitte / Gimona, Mario / Traweger, Andreas

    The American journal of sports medicine

    2023  Volume 51, Issue 2, Page(s) 331–342

    Abstract: Background: Despite significant advancements in surgical techniques to repair rotator cuff (RC) injuries, failure rates remain high and novel approaches to adequately overcome the natural biological limits of tendon and enthesis regeneration of the RC ... ...

    Abstract Background: Despite significant advancements in surgical techniques to repair rotator cuff (RC) injuries, failure rates remain high and novel approaches to adequately overcome the natural biological limits of tendon and enthesis regeneration of the RC are required. Small extracellular vesicles (sEVs) derived from the secretome of human multipotent mesenchymal stromal cells (MSCs) have been demonstrated to modulate inflammation and reduce fibrotic adhesions, and therefore their local application could improve outcomes after RC repair.
    Purpose: In this pilot study, we evaluated the efficacy of clinical-grade human umbilical cord (hUC) MSC-derived sEVs (hUC-MSC-sEVs) loaded onto a type 1 collagen scaffold in an ovine model of acute infraspinatus tendon injury to improve RC healing.
    Study design: Controlled laboratory study.
    Methods: sEVs were enriched from hUC-MSC culture media and were characterized by surface marker profiling. The immunomodulatory capacity was evaluated in vitro by T-cell proliferation assays, and particle count was determined by nanoparticle tracking analysis. Twelve skeletally mature sheep were subjected to partial infraspinatus tenotomy and enthesis debridement. The defects of 6 animals were treated with 2 × 10
    Results: CD3/CD28-stimulated T-cell proliferation was significantly inhibited by hUC-MSC-sEVs (
    Conclusion: A local, single-dose application of hUC-MSC-sEVs promoted tendon and enthesis healing in an ovine model of acute RC injury.
    Clinical relevance: Surgical repair of RC tears generally results in a clinical benefit for the patient; however, considerable rerupture rates have been reported. sEVs have potential as a cell-free biotherapeutic to improve healing outcomes after RC injury.
    MeSH term(s) Animals ; Sheep ; Humans ; Rotator Cuff/surgery ; Pilot Projects ; Collagen Type I/metabolism ; Rotator Cuff Injuries/surgery ; Mesenchymal Stem Cells/metabolism ; Extracellular Vesicles/pathology ; Umbilical Cord
    Chemical Substances Collagen Type I
    Language English
    Publishing date 2023-01-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 197482-8
    ISSN 1552-3365 ; 0363-5465
    ISSN (online) 1552-3365
    ISSN 0363-5465
    DOI 10.1177/03635465221145958
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1910: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Mechanics and dynamics of the cytoskeleton: a special issue stemming from the 2008 ECF Meeting/ FEBS Workshop (Mechanics and Dynamics of the Cytoskeleton) in Potsdam, Germany.

    Gimona, Mario

    Cell motility and the cytoskeleton

    2009  Volume 66, Issue 11, Page(s) ii–iii

    MeSH term(s) Animals ; Cytoskeleton/chemistry ; Cytoskeleton/metabolism ; Cytoskeleton/ultrastructure ; Humans
    Language English
    Publishing date 2009-11
    Publishing country United States
    Document type Introductory Journal Article
    ZDB-ID 283774-2
    ISSN 1097-0169 ; 0886-1544
    ISSN (online) 1097-0169
    ISSN 0886-1544
    DOI 10.1002/cm.20420
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1840: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Mechanics and dynamics of the cytoskeleton: a special issue stemming from the 2008 ECF Meeting/FEBS Workshop (Mechanics and Dynamics of the Cytoskeleton) in Potsdam, Germany.

    Gimona, Mario

    Cell motility and the cytoskeleton

    2009  Volume 66, Issue 10, Page(s) 769–770

    Abstract: Note that Part II of this special issue will appear in the following issue: Volume 66, Issue 11, 2009. ...

    Abstract Note that Part II of this special issue will appear in the following issue: Volume 66, Issue 11, 2009.
    MeSH term(s) Animals ; Biomechanical Phenomena ; Cytoskeleton ; Humans
    Language English
    Publishing date 2009-10
    Publishing country United States
    Document type Introductory Journal Article
    ZDB-ID 283774-2
    ISSN 1097-0169 ; 0886-1544
    ISSN (online) 1097-0169
    ISSN 0886-1544
    DOI 10.1002/cm.20415
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1840: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Manufacturing and characterization of extracellular vesicles from umbilical cord-derived mesenchymal stromal cells for clinical testing.

    Rohde, Eva / Pachler, Karin / Gimona, Mario

    Cytotherapy

    2019  Volume 21, Issue 6, Page(s) 581–592

    Abstract: Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) may deliver therapeutic effects that are comparable to their parental cells. MSC-EVs are promising agents for the treatment of a variety of diseases. To reach the intermediate ... ...

    Abstract Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) may deliver therapeutic effects that are comparable to their parental cells. MSC-EVs are promising agents for the treatment of a variety of diseases. To reach the intermediate goal of clinically testing safety and efficacy of EVs, strategies should strive for efficient translation of current EV research. On the basis of our in vitro an in vivo findings regarding the biological actions of EVs and our experience in manufacturing biological stem cell therapeutics for routine use and clinical testing, we discuss strategies of manufacturing and quality control of umbilical cord-derived MSC-EVs. We introduce guidelines of good manufacturing practice and their practicability along the path from the laboratory to the patient. We present aspects of manufacturing and final product quality testing and highlight the principle of "The process is the product." The approach presented in this perspective article may facilitate translational research during the development of complex biological EV-based therapeutics in a very early stage of manufacturing as well as during early clinical safety and proof-of-concept testing.
    MeSH term(s) Animals ; Biological Products/standards ; Biological Products/therapeutic use ; Cell Culture Techniques/methods ; Cell Culture Techniques/standards ; Clinical Trials as Topic ; Drug Stability ; Extracellular Vesicles/physiology ; Humans ; Mesenchymal Stem Cells/cytology ; Quality Control ; Umbilical Cord/cytology
    Chemical Substances Biological Products
    Language English
    Publishing date 2019-04-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2039821-9
    ISSN 1477-2566 ; 1465-3249
    ISSN (online) 1477-2566
    ISSN 1465-3249
    DOI 10.1016/j.jcyt.2018.12.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5601: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Calorimetry of extracellular vesicles fusion to single phospholipid membrane

    Grava Miriam / Helmy Sally / Gimona Mario / Parisse Pietro / Casalis Loredana / Brocca Paola / Rondelli Valeria

    Biomolecular Concepts, Vol 13, Iss 1, Pp 148-

    2022  Volume 155

    Abstract: Extracellular vesicles (EVs)-mediated communication relies not only on the delivery of complex molecular cargoes as lipids, proteins, genetic material, and metabolites to their target cells but also on the modification of the cell surface local ... ...

    Abstract Extracellular vesicles (EVs)-mediated communication relies not only on the delivery of complex molecular cargoes as lipids, proteins, genetic material, and metabolites to their target cells but also on the modification of the cell surface local properties induced by the eventual fusion of EVs’ membranes with the cells’ plasma membrane. Here we applied scanning calorimetry to study the phase transition of single phospholipid (DMPC) monolamellar vesicles, investigating the thermodynamical effects caused by the fusion of doping amounts of mesenchymal stem cells-derived EVs. Specifically, we studied EVs-induced consequences on the lipids distributed in the differently curved membrane leaflets, having different density and order. The effect of EV components was found to be not homogeneous in the two leaflets, the inner (more disordered one) being mainly affected. Fusion resulted in phospholipid membrane flattening associated with lipid ordering, while the transition cooperativity, linked to membrane domains’ coexistence during the transition process, was decreased. Our results open new horizons for the investigation of the peculiar effects of EVs of different origins on target cell membrane properties and functionality.
    Keywords Biology (General) ; QH301-705.5
    Subject code 571 ; 500
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher De Gruyter
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Extracellular Vesicles in Inner Ear Therapies-Pathophysiological, Manufacturing, and Clinical Considerations.

    Warnecke, Athanasia / Staecker, Hinrich / Rohde, Eva / Gimona, Mario / Giesemann, Anja / Szczepek, Agnieszka J / Di Stadio, Arianna / Hochmair, Ingeborg / Lenarz, Thomas

    Journal of clinical medicine

    2022  Volume 11, Issue 24

    Abstract: 1) Background: Sensorineural hearing loss is a common and debilitating condition. To date, comprehensive pharmacologic interventions are not available. The complex and diverse molecular pathology that underlies hearing loss may limit our ability to ... ...

    Abstract (1) Background: Sensorineural hearing loss is a common and debilitating condition. To date, comprehensive pharmacologic interventions are not available. The complex and diverse molecular pathology that underlies hearing loss may limit our ability to intervene with small molecules. The current review foccusses on the potential for the use of extracellular vesicles in neurotology. (2) Methods: Narrative literature review. (3) Results: Extracellular vesicles provide an opportunity to modulate a wide range of pathologic and physiologic pathways and can be manufactured under GMP conditions allowing for their application in the human inner ear. The role of inflammation in hearing loss with a focus on cochlear implantation is shown. How extracellular vesicles may provide a therapeutic option for complex inflammatory disorders of the inner ear is discussed. Additionally, manufacturing and regulatory issues that need to be addressed to develop EVs as advanced therapy medicinal product for use in the inner ear are outlined. (4) Conclusion: Given the complexities of inner ear injury, novel therapeutics such as extracellular vesicles could provide a means to modulate inflammation, stress pathways and apoptosis in the inner ear.
    Language English
    Publishing date 2022-12-15
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm11247455
    Database MEDical Literature Analysis and Retrieval System OnLINE

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