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Article ; Online: Candida makes a lasting impression in COVID-19.

Lagree, Katherine / Chen, Peter

2023 Volume 24, Issue 11, Page(s) 1782–1784

MeSH term(s)	Humans ; Candida ; COVID-19 ; Candida albicans
Language	English
Publishing date	2023-10-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2016987-5
ISSN	1529-2916 ; 1529-2908
ISSN (online)	1529-2916
ISSN	1529-2908
DOI	10.1038/s41590-023-01648-1
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Article ; Online: Candida-induced asthma steps up to the plate-lets.

Lagree, Katherine / Underhill, David M

Immunity

2021 Volume 54, Issue 11, Page(s) 2442–2444

Abstract: Fungal proteases are well-known allergens. In this issue of Immunity, Wu et al. (2021) observe that allergic airway responses to Candida albicans are mediated by the peptide toxin candidalysin rather than proteases. Candidalysin promotes these responses ... ...

Abstract	Fungal proteases are well-known allergens. In this issue of Immunity, Wu et al. (2021) observe that allergic airway responses to Candida albicans are mediated by the peptide toxin candidalysin rather than proteases. Candidalysin promotes these responses by stimulating platelets to release the Wnt antagonist Dickkopf-1.
MeSH term(s)	Asthma ; Candida ; Candida albicans ; Humans
Language	English
Publishing date	2021-11-07
Publishing country	United States
Document type	Journal Article ; Comment
ZDB-ID	1217235-2
ISSN	1097-4180 ; 1074-7613
ISSN (online)	1097-4180
ISSN	1074-7613
DOI	10.1016/j.immuni.2021.10.014
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Zs.A 4227: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 69: Show issues

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Article ; Online: Fungal Biofilms: Inside Out.

Lagree, Katherine / Mitchell, Aaron P

Microbiology spectrum

2017 Volume 5, Issue 2

Abstract: We focus this article on turning a biofilm inside out. The "inside" of the biofilm comprises the individual biofilm-related phenotypes, their environmental drivers and genetic determinants, and the coordination of gene functions through transcriptional ... ...

Abstract	We focus this article on turning a biofilm inside out. The "inside" of the biofilm comprises the individual biofilm-related phenotypes, their environmental drivers and genetic determinants, and the coordination of gene functions through transcriptional regulators. Investigators have viewed the inside of the biofilm through diverse approaches, and this article will attempt to capture the essence of many. The ultimate goal is to connect the inside to the "outside," which we view as biofilm structure, development, pharmacological attributes, and medical impact.
MeSH term(s)	Biofilms/growth & development ; Fungi/physiology
Language	English
Publishing date	2017-04
Publishing country	United States
Document type	Journal Article ; Review
ISSN	2165-0497
ISSN (online)	2165-0497
DOI	10.1128/microbiolspec.FUNK-0024-2016
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A New Expansion Microscopy Method Optimized for Microbiology.

Cheng, Zhangyu / Stefani, Caroline / Skillman, Thomas / Klimas, Aleksandra / Lee, Aramchan / DiBernardo, Emma F / Brown, Karina M / Milman, Tatyana / Gallagher, Brendan R / Lagree, Katherine / Jena, Bhanu P / Pulido, Jose / Mitchell, Aaron P / Filler, Scott G / Hiller, Luisa / Lacy-Hulbert, Adam / Zhao, Yongxin

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

2023 Volume 29, Issue 29 Suppl 1, Page(s) 994

Language	English
Publishing date	2023-08-23
Publishing country	England
Document type	Journal Article
ZDB-ID	1385710-1
ISSN	1435-8115 ; 1431-9276
ISSN (online)	1435-8115
ISSN	1431-9276
DOI	10.1093/micmic/ozad067.498
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Clarifying and Imaging Candida albicans Biofilms.

Lanni, Frederick / Lagree, Katherine / Huang, Manning Y / Yan, Lan / Woolford, Carol A / Mitchell, Aaron P

Journal of visualized experiments : JoVE

2020 , Issue 157

Abstract: The microbial fungus Candida albicans can undergo a change from commensal colonization to virulence that is strongly correlated with its ability to switch from yeast-form growth to hyphal growth. Cells initiating this process become adherent to surfaces ... ...

Abstract	The microbial fungus Candida albicans can undergo a change from commensal colonization to virulence that is strongly correlated with its ability to switch from yeast-form growth to hyphal growth. Cells initiating this process become adherent to surfaces as well as to each other, with the resulting development of a biofilm colony. This commonly occurs not only on mucosal tissue surfaces in yeast infections, but also on medical implants such as catheters. It is well known that biofilm cells are resistant to antifungal drugs, and that cells that shed from the biofilm can lead to dangerous systemic infections. Biofilms range from heavily translucent to opaque due to refractive heterogeneity. Therefore, fungal biofilms are difficult to study by optical microscopy. To visualize internal structural, cellular, and subcellular features, we clarify fixed intact biofilms by stepwise solvent exchange to a point of optimal refractive index matching. For C. albicans biofilms, sufficient clarification is attained with methyl salicylate (n = 1.537) to enable confocal microscopy from apex to base in 600 µm biofilms with little attenuation. In this visualization protocol we outline phase contrast refractometry, the growth of laboratory biofilms, fixation, staining, solvent exchange, the setup for confocal fluorescence microscopy, and representative results.
MeSH term(s)	Biofilms/growth & development ; Candida albicans/physiology ; Hyphae/physiology ; Imaging, Three-Dimensional ; Microscopy, Confocal ; Mutation/genetics ; Refractometry
Language	English
Publishing date	2020-03-06
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/60718
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Microscopy of fungal biofilms.

Lagree, Katherine / Desai, Jigar V / Finkel, Jonathan S / Lanni, Frederick

Current opinion in microbiology

2018 Volume 43, Page(s) 100–107

Abstract: Fungal biofilms are heterogeneous, surface-associated colonies comprised of filamentous hyphae (chains of elongated cells), pseudohyphal cells, yeast-form cells, and various forms of extracellular matrix. When grown on a substratum under liquid culture ... ...

Abstract	Fungal biofilms are heterogeneous, surface-associated colonies comprised of filamentous hyphae (chains of elongated cells), pseudohyphal cells, yeast-form cells, and various forms of extracellular matrix. When grown on a substratum under liquid culture medium, the microbial fungus Candida albicans forms dense biofilms that range in thickness from 100 to 600μm. Apical hyphae in the medium and invasive hyphae in the substratum may add greatly to the thickness and complexity of the biofilm. Because of the heterogeneity of the structure, and the large refractive index differences between cell walls, cytoplasm, and medium, fungal biofilms appear optically opaque. For fixed specimens that can be transferred out of an aqueous medium, refractive index matching methods provide a high degree of clarification. Confocal scanning, 2-photon scanning, or selective-plane illumination microscopy then can be used to obtain high-quality image data spanning the full thickness of the biofilm. Using refractive index matching and confocal microscopy, we have imaged many interesting features within wild-type, mutant, and engineered biofilms, including cellular phenotypes that vary with position, the effect of growth conditions, and gene expression through reporter constructs. This approach greatly expands the range of microscopical studies, allowing researchers to observe and quantify specific phenomena within medically or industrially relevant forms of microbial growth.
MeSH term(s)	Biofilms ; Candida albicans/genetics ; Candida albicans/growth & development ; Candida albicans/physiology ; Candida albicans/ultrastructure ; Fungi/growth & development ; Fungi/physiology ; Gene Expression Regulation, Fungal ; Hyphae/genetics ; Hyphae/ultrastructure ; Microscopy, Confocal/methods
Language	English
Publishing date	2018-02-04
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	1418474-6
ISSN	1879-0364 ; 1369-5274
ISSN (online)	1879-0364
ISSN	1369-5274
DOI	10.1016/j.mib.2017.12.008
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Zs.A 5514: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 51: Show issues

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Article ; Online: Impact of surface topography on biofilm formation by Candida albicans.

Lagree, Katherine / Mon, Htwe H / Mitchell, Aaron P / Ducker, William A

PloS one

2018 Volume 13, Issue 6, Page(s) e0197925

Abstract: Candida albicans is a fungal pathogen that causes serious biofilm-based infections. Here we have asked whether surface topography may affect C. albicans biofilm formation. We tested biofilm growth of the prototypical wild-type strain SC5314 on a series ... ...

Abstract	Candida albicans is a fungal pathogen that causes serious biofilm-based infections. Here we have asked whether surface topography may affect C. albicans biofilm formation. We tested biofilm growth of the prototypical wild-type strain SC5314 on a series of polydimethylsiloxane (PDMS) solids. The surfaces were prepared with monolayer coatings of monodisperse spherical silica particles that were fused together into a film using silica menisci. The surface topography was varied by varying the diameter of the silica particles that were used to form the film. Biofilm formation was observed to be a strong function of particle size. In the particle size range 4.0-8.0 μm, there was much more biofilm than in the size range 0.5-2.0 μm. The behavior of a clinical isolate from a clade separate from SC5314, strain p76067, showed results similar to that of SC5314. Our results suggest that topographic coatings may be a promising approach to reduce C. albicans biofilm infections.
MeSH term(s)	Biofilms/drug effects ; Biofilms/growth & development ; Candida albicans/drug effects ; Candida albicans/physiology ; Dimethylpolysiloxanes/chemistry ; Dimethylpolysiloxanes/pharmacology ; Nylons/chemistry ; Nylons/pharmacology ; Silicon Dioxide/chemistry ; Surface Properties
Chemical Substances	Dimethylpolysiloxanes ; Nylons ; poly(dimethylsiloxane)-polyamide copolymer ; Silicon Dioxide (7631-86-9)
Language	English
Publishing date	2018-06-18
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0197925
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: MicroMagnify: a multiplexed expansion microscopy method for pathogens and infected tissues.

Zhao, Yongxin / Cheng, Zhangyu / Stefani, Caroline / Skillman, Thomas / Klimas, Aleksandra / Lee, Aramchan / DiBernardo, Emma / Mueller Brown, Karina / Milman, Tatyana / Gallagher, Brendan / Lagree, Katherine / Jena, Bhanu / Pulido, Jose / Filler, Scott / Mitchell, Aaron / Hiller, Luisa / Lacy-Hulbert, Adam

Research square

2023

Abstract: Super-resolution optical imaging tools are crucial in microbiology to understand the complex structures and behavior of microorganisms such as bacteria, fungi, and viruses. However, the capabilities of these tools, particularly when it comes to imaging ... ...

Abstract	Super-resolution optical imaging tools are crucial in microbiology to understand the complex structures and behavior of microorganisms such as bacteria, fungi, and viruses. However, the capabilities of these tools, particularly when it comes to imaging pathogens and infected tissues, remain limited. We developed µMagnify, a nanoscale multiplexed imaging method for pathogens and infected tissues that are derived from an expansion microscopy technique with a universal biomolecular anchor. We formulated an enzyme cocktail specifically designed for robust cell wall digestion and expansion of microbial cells without distortion while efficiently retaining biomolecules suitable for high-plex fluorescence imaging with nanoscale precision. Additionally, we developed an associated virtual reality tool to facilitate the visualization and navigation of complex three-dimensional images generated by this method in an immersive environment allowing collaborative exploration among researchers around the world. µMagnify is a valuable imaging platform for studying how microbes interact with their host systems and enables development of new diagnosis strategies against infectious diseases.
Language	English
Publishing date	2023-03-06
Publishing country	United States
Document type	Preprint
DOI	10.21203/rs.3.rs-2637060/v1
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Article ; Online: Glucose-enhanced oxidative stress resistance-A protective anticipatory response that enhances the fitness of Candida albicans during systemic infection.

Larcombe, Daniel E / Bohovych, Iryna M / Pradhan, Arnab / Ma, Qinxi / Hickey, Emer / Leaves, Ian / Cameron, Gary / Avelar, Gabriela M / de Assis, Leandro J / Childers, Delma S / Bain, Judith M / Lagree, Katherine / Mitchell, Aaron P / Netea, Mihai G / Erwig, Lars P / Gow, Neil A R / Brown, Alistair J P

PLoS pathogens

2023 Volume 19, Issue 7, Page(s) e1011505

Abstract: Most microbes have developed responses that protect them against stresses relevant to their niches. Some that inhabit reasonably predictable environments have evolved anticipatory responses that protect against impending stresses that are likely to be ... ...

Abstract	Most microbes have developed responses that protect them against stresses relevant to their niches. Some that inhabit reasonably predictable environments have evolved anticipatory responses that protect against impending stresses that are likely to be encountered in their niches-termed "adaptive prediction". Unlike yeasts such as Saccharomyces cerevisiae, Kluyveromyces lactis and Yarrowia lipolytica and other pathogenic Candida species we examined, the major fungal pathogen of humans, Candida albicans, activates an oxidative stress response following exposure to physiological glucose levels before an oxidative stress is even encountered. Why? Using competition assays with isogenic barcoded strains, we show that "glucose-enhanced oxidative stress resistance" phenotype enhances the fitness of C. albicans during neutrophil attack and during systemic infection in mice. This anticipatory response is dependent on glucose signalling rather than glucose metabolism. Our analysis of C. albicans signalling mutants reveals that the phenotype is not dependent on the sugar receptor repressor pathway, but is modulated by the glucose repression pathway and down-regulated by the cyclic AMP-protein kinase A pathway. Changes in catalase or glutathione levels do not correlate with the phenotype, but resistance to hydrogen peroxide is dependent on glucose-enhanced trehalose accumulation. The data suggest that the evolution of this anticipatory response has involved the recruitment of conserved signalling pathways and downstream cellular responses, and that this phenotype protects C. albicans from innate immune killing, thereby promoting the fitness of C. albicans in host niches.
MeSH term(s)	Humans ; Animals ; Mice ; Candida albicans ; Glucose/metabolism ; Oxidative Stress/physiology ; Neutrophils ; Saccharomyces cerevisiae/metabolism ; Fungal Proteins/metabolism
Chemical Substances	Glucose (IY9XDZ35W2) ; Fungal Proteins
Language	English
Publishing date	2023-07-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7374
ISSN (online)	1553-7374
ISSN	1553-7374
DOI	10.1371/journal.ppat.1011505
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Article: Microscopy of fungal biofilms

Lagree, Katherine / Frederick Lanni / Jigar V Desai / Jonathan S Finkel

Elsevier Ltd Current opinion in microbiology. 2018 June, v. 43

2018

Abstract	Fungal biofilms are heterogeneous, surface-associated colonies comprised of filamentous hyphae (chains of elongated cells), pseudohyphal cells, yeast-form cells, and various forms of extracellular matrix. When grown on a substratum under liquid culture medium, the microbial fungus Candida albicans forms dense biofilms that range in thickness from 100 to 600μm. Apical hyphae in the medium and invasive hyphae in the substratum may add greatly to the thickness and complexity of the biofilm. Because of the heterogeneity of the structure, and the large refractive index differences between cell walls, cytoplasm, and medium, fungal biofilms appear optically opaque. For fixed specimens that can be transferred out of an aqueous medium, refractive index matching methods provide a high degree of clarification. Confocal scanning, 2-photon scanning, or selective-plane illumination microscopy then can be used to obtain high-quality image data spanning the full thickness of the biofilm. Using refractive index matching and confocal microscopy, we have imaged many interesting features within wild-type, mutant, and engineered biofilms, including cellular phenotypes that vary with position, the effect of growth conditions, and gene expression through reporter constructs. This approach greatly expands the range of microscopical studies, allowing researchers to observe and quantify specific phenomena within medically or industrially relevant forms of microbial growth.
Keywords	biofilm ; Candida albicans ; cell walls ; confocal microscopy ; culture media ; cytoplasm ; extracellular matrix ; fungi ; gene expression ; hyphae ; lighting ; liquids ; microbial growth ; mutants ; phenotype ; refractive index ; researchers
Language	English
Dates of publication	2018-06
Size	p. 100-107.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1418474-6
ISSN	1879-0364 ; 1369-5274
ISSN (online)	1879-0364
ISSN	1369-5274
DOI	10.1016/j.mib.2017.12.008
Database	NAL-Catalogue (AGRICOLA)

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