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Article ; Online: Cellulolytic and Ethanologenic Evaluation of

Azhar, Sana / Aihetasham, Ayesha / Chaudhary, Asma / Hussain, Zawar / Abdul Rehman, Rahat / Abbas, Ghulam / Alharbi, Sulaiman Ali / Ansari, Mohammad Javed / Qamer, Samina

ACS omega

2024 Volume 9, Issue 10, Page(s) 12084–12100

Abstract: Cellulose is the basic component of lignocellulosic biomass (LCB) making it a suitable substrate for bioethanol fermentation. Cellulolytic and ethanologenic bacteria possess cellulases that convert cellulose to glucose, which in turn yields ethanol ... ...

Abstract	Cellulose is the basic component of lignocellulosic biomass (LCB) making it a suitable substrate for bioethanol fermentation. Cellulolytic and ethanologenic bacteria possess cellulases that convert cellulose to glucose, which in turn yields ethanol subsequently.
Language	English
Publishing date	2024-02-29
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.3c10030
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Insights of OPs and PYR cytotoxic potential Invitro and genotoxic impact on PON1 genetic variant among exposed workers in Pakistan.

Imran, Iffat / Ansari, Asma / Saleem, Saima / Azhar, Abid / Zehra, Sitwat

Scientific reports

2022 Volume 12, Issue 1, Page(s) 9498

Abstract: Different pesticide chemicals are used to enhance crop yield by protecting from pests. Organophosphate (OPs) and Pyrethroid (PYR) are used in fields of Sanghar, Sindh Pakistan. PON1 an antioxidant enzyme implicated in OPs detoxification may predispose by ...

Abstract	Different pesticide chemicals are used to enhance crop yield by protecting from pests. Organophosphate (OPs) and Pyrethroid (PYR) are used in fields of Sanghar, Sindh Pakistan. PON1 an antioxidant enzyme implicated in OPs detoxification may predispose by OPs chronic exposure. This study was conducted to evaluate the toxic potential of active pesticide chemicals at cellular and genetic levels. To examine toxic potential, locally consumed pesticide n = 2 and reference pesticide compounds organophosphate (OPs): Chloropyrifos, Malathion and Pyrethroid (PYR): Cyprmethrin, Cyhalothrin n = 4 were tested against NIH 3T3 cells using MTS assay. Local consumer pesticides demonstrated relevance for half-maximum inhibitory concentration (IC50) 0.00035 mg/mL with selected compound. Malathion IC50 exhibited the highest cytotoxicity among four compounds at 0.0005 mg/mL. On genotoxicity analysis in exposed subjects n = 100 genotypes and alleles n = 200 exhibited significant differences in genotypic and allelic frequencies of pesticide exposed subjects and controls n = 150 (X
MeSH term(s)	Animals ; Aryldialkylphosphatase/genetics ; Aryldialkylphosphatase/metabolism ; DNA Damage ; Humans ; Malathion ; Mice ; Occupational Exposure ; Organophosphates/toxicity ; Organophosphorus Compounds/toxicity ; Pakistan ; Pesticides/toxicity ; Pyrethrins/toxicity
Chemical Substances	Organophosphates ; Organophosphorus Compounds ; Pesticides ; Pyrethrins ; Aryldialkylphosphatase (EC 3.1.8.1) ; PON1 protein, human (EC 3.1.8.1) ; PON1 protein, mouse (EC 3.1.8.1) ; Malathion (U5N7SU872W)
Language	English
Publishing date	2022-06-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-13454-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Synergistic evaluation of Moringa oleifera extract and ß-lactam antibiotic to restore the susceptibility of methicillin-resistant Staphylococcus aureus.

Vankwani, Soma / Ansari, Asma / Azhar, Abid / Galani, Saddia

Molecular biology reports

2021 Volume 49, Issue 1, Page(s) 421–432

Abstract: Introduction: The antibiotic resistance has become a major threat to global health. The combinatorial use of two or more compounds to develop a new formulation may overcome the emerging cases of drug resistance. Moringa oleifera has been utilized as a ... ...

Abstract	Introduction: The antibiotic resistance has become a major threat to global health. The combinatorial use of two or more compounds to develop a new formulation may overcome the emerging cases of drug resistance. Moringa oleifera has been utilized as a strong nutritional, immunomodulator and therapeutic agent for decades. In this study, different parts of Moringa oleifera were screened for bioactive compounds that can act as a resistance modifying agent for multi-drug resistant organisms (MDROs). Methodology: Initially, the combined effect of stem bark extracts and ampicillin was calculated by checkerboard assay. Active compounds of effective extract were assessed by High Performance Liquid Chromatography (HPLC). Minimal Inhibitory Concentration (MIC) and Fractional Inhibitory Concentration Index (FICI) were calculated to evaluate the synergistic behavior of stem bark extract with ampicillin. To study the blocking of resistance pathways of Methicillin-Resistant Staphylococcus aureus (MRSA) western blot was performed. Results: The results revealed that stem bark has significant anti-MRSA activity. The methanolic extract of stem bark in combination with ampicillin showed the highest synergistic effect (FICI value ≤ 0.237) against MRSA. Killing kinetics and membrane potential of ampicillin alone and in combination revealed an increase in the inhibitory potential of ampicillin against MRSA. Decolourization in iodometric assay confirmed the inhibition of β-lactamase, western blot results confirmed the blocking of penicillin-binding protein (PBP2a) expression with the restoration of MRSA sensitivity against β-lactams. Conclusion: It can be concluded that methanolic extract of Moringa oleifera stem bark has bioactive compounds and can be used as an adjuvant with antibiotics to modify the resistance of MDROs.
MeSH term(s)	Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Chromatography, High Pressure Liquid ; Dose-Response Relationship, Drug ; Drug Synergism ; Methicillin-Resistant Staphylococcus aureus/drug effects ; Microbial Sensitivity Tests ; Moringa oleifera/chemistry ; Phytochemicals/chemistry ; Phytochemicals/pharmacology ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; beta-Lactams/chemistry ; beta-Lactams/pharmacology
Chemical Substances	Anti-Bacterial Agents ; Phytochemicals ; Plant Extracts ; beta-Lactams
Language	English
Publishing date	2021-11-22
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	186544-4
ISSN	1573-4978 ; 0301-4851
ISSN (online)	1573-4978
ISSN	0301-4851
DOI	10.1007/s11033-021-06889-7
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Article ; Online: High sensitivity detection of different pH ranges with rGO-nanocomposite coated eFBG sensor.

Shadab, Azhar / Ansari, Md Tauseef Iqbal / Raghuwanshi, Sanjeev Kumar / Kumar, Santosh

Applied optics

2023 Volume 62, Issue 19, Page(s) 5334–5341

Abstract: In the proposed work, a highly sensitive reduced graphene oxide (rGO) coated etched fiber Bragg grating (eFBG) pH sensor is developed and characterized. To create the sensing probe, a nanocomposite layer of rGO is coated over the unclad area of the eFBG. ...

Abstract	In the proposed work, a highly sensitive reduced graphene oxide (rGO) coated etched fiber Bragg grating (eFBG) pH sensor is developed and characterized. To create the sensing probe, a nanocomposite layer of rGO is coated over the unclad area of the eFBG. The analysis of rGO material has been done using different characterization tools such as UV-VIS-NIR spectroscopy, x-ray diffraction (XRD), and field emission scanning electron microscopy (FESEM). Experiments are performed using pH samples ranging from pH 2 to pH 12 to validate the operational sensing range of the proposed sensor. The effectiveness of the proposed sensor is evaluated with various pH values by monitoring the shift in the resonance peak of the sensor's reflection spectrum in a real-time interrogation system. The sensor performs well in both low and high pH ranges, with a maximum sensitivity of 0.232 nm/pH at pH 12. Due to a shift in the rGO's optical band-gap at both low and high pH values in the samples, the sensor can detect minimal changes in concentration. In the reflected spectrum, the Bragg wavelength (
Language	English
Publishing date	2023-09-14
Publishing country	United States
Document type	Journal Article
ISSN	1539-4522
ISSN (online)	1539-4522
DOI	10.1364/AO.492750
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Article ; Online: Experiences, awareness, perceptions and attitudes of women and girls towards menstrual hygiene management and safe menstrual products in Pakistan.

Malik, Madeeha / Hashmi, Ayisha / Hussain, Azhar / Khan, Waleed / Jahangir, Nabia / Malik, Anam / Ansari, Naima

Frontiers in public health

2023 Volume 11, Page(s) 1242169

Abstract: Background: The taboo of menstruation, lack of discussion on puberty, misinformation and poor awareness on menstrual hygiene management and limited access to safe menstrual products can negatively impact the physical and mental health of women and girls ...

Abstract	Background: The taboo of menstruation, lack of discussion on puberty, misinformation and poor awareness on menstrual hygiene management and limited access to safe menstrual products can negatively impact the physical and mental health of women and girls residing in low middle income countries. Aim: The aim of the study was to explore the experiences, awareness, perceptions and attitudes of women and girls towards menstrual hygiene management in Pakistan. Moreover, the study also assessed consumer satisfaction towards locally manufactured organic menstrual products. Methods: A descriptive cross-sectional study design was used with a sample of 400 women and girls selected through convenience sampling from high schools, universities, outpatient department and gynaecological clinics located in 2 cities, i.e., Islamabad and Rawalpindi, Pakistan. A pre-structured questionnaire was used to explore experiences, awareness, perceptions and attitudes of women and girls towards menstrual hygiene management. Moreover, each respondent was provided with sample of locally designed and manufactured organic menstrual hygiene & wellness kit by a group of women researchers named "FemPure" including organic sanitary pads, feminine wellness mist and feminine wellness wash. The respondents were asked to use the products and a telephonic follow-up was conducted to assess consumer satisfaction for the products after a period of 1 month. Data was analyzed statistically using SPSS 21. Results: The results of the study reported that 86.2% ( Conclusion: The study concluded that majority of women and girls faced menstrual hygiene issues during and after the cycle and were eager to receive information on MHM and use organic rash free menstrual wellness products which could be ordered via mobile app. The respondents were satisfied after the use of FemPure organic menstrual products.
Language	English
Publishing date	2023-09-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2711781-9
ISSN	2296-2565 ; 2296-2565
ISSN (online)	2296-2565
ISSN	2296-2565
DOI	10.3389/fpubh.2023.1242169
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Article ; Online: Deodorant aerosol spray can in the rectum: a potential fire hazard during surgery.

Kumarasamy, Sivaraman / Kaman, Lileswar / Ansari, Azhar / Hazarika, Amarjyoti

BMJ case reports

2021 Volume 14, Issue 5

Abstract: Rectal foreign bodies are not uncommon and often pose a serious challenge to surgeons. In majority, the objects are inserted by self in children, psychiatric patients and for sexual gratification in adults. Various rectal foreign bodies have been ... ...

Abstract	Rectal foreign bodies are not uncommon and often pose a serious challenge to surgeons. In majority, the objects are inserted by self in children, psychiatric patients and for sexual gratification in adults. Various rectal foreign bodies have been reported. Deodorant aerosol spray can in the rectum has not been reported previously. Danger of aerosol dispenser can is the risk of fire and explosion. Especially during any surgical procedures to remove the foreign body using cautery or any energy devices. We report of a rare foreign body of deodorant aerosol spray can in the rectum in a young male patient, which was removed under general anaesthesia.
MeSH term(s)	Adult ; Aerosols ; Child ; Deodorants/adverse effects ; Foreign Bodies/diagnostic imaging ; Humans ; Male ; Rectal Diseases ; Rectum/surgery
Chemical Substances	Aerosols ; Deodorants
Language	English
Publishing date	2021-05-24
Publishing country	England
Document type	Case Reports ; Journal Article
ISSN	1757-790X
ISSN (online)	1757-790X
DOI	10.1136/bcr-2020-241538
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: To Evaluate the Efficiency of ChatGPT in Medical Education

Shirjeel Husain / Zaid Ansari / Azhar Hussain / Sahar Abbasi / Tahera Ayoob / Rimsha Mujahid

Annals of Abbasi Shaheed Hospital and Karachi Medical & Dental College, Vol 28, Iss

An Analysis of MCQ-Based Learning and Assessment

2023 Volume 04

Abstract: Objective: This study aimed to evaluate the potential of ChatGPT to help students for their assess ments via MCQ at different level of cognition by using different subjects of Internal medicine. Methods: This cross-sectional study was conducted in the ... ...

Abstract	Objective: This study aimed to evaluate the potential of ChatGPT to help students for their assess ments via MCQ at different level of cognition by using different subjects of Internal medicine. Methods: This cross-sectional study was conducted in the Department of Internal Medicine in col laboration with post graduate medical education department from June 2023 to August 2023. An MCQ bank was established from three books of MCQ’s on subject of Internal Medicine. Total 1428 MCQ’s were followed for scrutiny and 307 MCQ’s were selected for the assigned task. The selected MCQ’s were manually entered one by one in a fresh Chat GPT session. The response was noted against the replies given in respective MCQ’s book and marked as correct, not correct or partially correct. MCQ’s were categorized as per chapters in Internal medicine and as per cognition level of MCQ’s i.e. C1, C2 and C3. Data was analyzed on SPSS version 21.00. Results: Chat GPT replied with 199 correct replies while 98 were wrong and 10 were partially correct. Chat GPT scored 64% overall in all categories. At level of cognition, it solved C2 MCQ’s by 80 % but scored 69% and 54% in C1 and C3 categories respectively. Chat GPT replied with 80% accuracy for C2 level MCQ’s while results were low for C3 category at around 54%. C1 also had low percentage of correct answers standing close to 69.8%. Almost all subjects showed healthy responses around the mean except for endocrinology and hematology where responses are below 60% and 40% respectively. Conclusion: This study findings suggest that ChatGPT is a useful tool for students and medical educationist with its current framework but a subtle approach should be inclined towards its role in future.
Keywords	Medical education ; Medicine (General) ; R5-920 ; Dentistry ; RK1-715
Subject code	150
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	KARACHI MEDICAL AND DENTAL COLLEGE
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: To Evaluate the Efficiency of ChatGPT in Medical Education

Shirjeel Husain / Zaid Ansari / Azhar Hussain / Sahar Abbasi / Tahera Ayoob / Rimsha Mujahid

Annals of Abbasi Shaheed Hospital and Karachi Medical & Dental College, Vol 28, Iss 04, Pp 194-

An Analysis of MCQ-Based Learning and Assessment

2023 Volume 200

Abstract	Objective: This study aimed to evaluate the potential of ChatGPT to help students for their assess ments via MCQ at different level of cognition by using different subjects of Internal medicine. Methods: This cross-sectional study was conducted in the Department of Internal Medicine in col laboration with post graduate medical education department from June 2023 to August 2023. An MCQ bank was established from three books of MCQ’s on subject of Internal Medicine. Total 1428 MCQ’s were followed for scrutiny and 307 MCQ’s were selected for the assigned task. The selected MCQ’s were manually entered one by one in a fresh Chat GPT session. The response was noted against the replies given in respective MCQ’s book and marked as correct, not correct or partially correct. MCQ’s were categorized as per chapters in Internal medicine and as per cognition level of MCQ’s i.e. C1, C2 and C3. Data was analyzed on SPSS version 21.00. Results: Chat GPT replied with 199 correct replies while 98 were wrong and 10 were partially correct. Chat GPT scored 64% overall in all categories. At level of cognition, it solved C2 MCQ’s by 80 % but scored 69% and 54% in C1 and C3 categories respectively. Chat GPT replied with 80% accuracy for C2 level MCQ’s while results were low for C3 category at around 54%. C1 also had low percentage of correct answers standing close to 69.8%. Almost all subjects showed healthy responses around the mean except for endocrinology and hematology where responses are below 60% and 40% respectively. Conclusion: This study findings suggest that ChatGPT is a useful tool for students and medical educationist with its current framework but a subtle approach should be inclined towards its role in future.
Keywords	medical education ; Medicine (General) ; R5-920 ; Dentistry ; RK1-715
Subject code	150
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	KARACHI MEDICAL AND DENTAL COLLEGE
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Insights of OPs and PYR cytotoxic potential Invitro and genotoxic impact on PON1 genetic variant among exposed workers in Pakistan

Iffat Imran / Asma Ansari / Saima Saleem / Abid Azhar / Sitwat Zehra

Scientific Reports, Vol 12, Iss 1, Pp 1-

2022 Volume 7

Abstract: Abstract Different pesticide chemicals are used to enhance crop yield by protecting from pests. Organophosphate (OPs) and Pyrethroid (PYR) are used in fields of Sanghar, Sindh Pakistan. PON1 an antioxidant enzyme implicated in OPs detoxification may ... ...

Abstract	Abstract Different pesticide chemicals are used to enhance crop yield by protecting from pests. Organophosphate (OPs) and Pyrethroid (PYR) are used in fields of Sanghar, Sindh Pakistan. PON1 an antioxidant enzyme implicated in OPs detoxification may predispose by OPs chronic exposure. This study was conducted to evaluate the toxic potential of active pesticide chemicals at cellular and genetic levels. To examine toxic potential, locally consumed pesticide n = 2 and reference pesticide compounds organophosphate (OPs): Chloropyrifos, Malathion and Pyrethroid (PYR): Cyprmethrin, Cyhalothrin n = 4 were tested against NIH 3T3 cells using MTS assay. Local consumer pesticides demonstrated relevance for half-maximum inhibitory concentration (IC50) 0.00035 mg/mL with selected compound. Malathion IC50 exhibited the highest cytotoxicity among four compounds at 0.0005 mg/mL. On genotoxicity analysis in exposed subjects n = 100 genotypes and alleles n = 200 exhibited significant differences in genotypic and allelic frequencies of pesticide exposed subjects and controls n = 150 (X2 = 22.9, p = 0.001). Screening of genotypes were performed by PCR- RFLP. Statistical assessment carried out using online software and tools. Results suggested that higher heterozygous genotype A/G (74%) may confer low PON1 metabolic activity towards pesticides in exposed subjects. Findings could be helpful to establish health plans by avoiding toxic chemicals that harming exposed population.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Synergistic evaluation of Moringa oleifera extract and ß-lactam antibiotic to restore the susceptibility of methicillin-resistant Staphylococcus aureus

Vankwani, Soma / Ansari, Asma / Azhar, Abid / Galani, Saddia

Molecular biology reports. 2022 Jan., v. 49, no. 1

2022

Abstract: INTRODUCTION: The antibiotic resistance has become a major threat to global health. The combinatorial use of two or more compounds to develop a new formulation may overcome the emerging cases of drug resistance. Moringa oleifera has been utilized as a ... ...

Abstract	INTRODUCTION: The antibiotic resistance has become a major threat to global health. The combinatorial use of two or more compounds to develop a new formulation may overcome the emerging cases of drug resistance. Moringa oleifera has been utilized as a strong nutritional, immunomodulator and therapeutic agent for decades. In this study, different parts of Moringa oleifera were screened for bioactive compounds that can act as a resistance modifying agent for multi-drug resistant organisms (MDROs). METHODOLOGY: Initially, the combined effect of stem bark extracts and ampicillin was calculated by checkerboard assay. Active compounds of effective extract were assessed by High Performance Liquid Chromatography (HPLC). Minimal Inhibitory Concentration (MIC) and Fractional Inhibitory Concentration Index (FICI) were calculated to evaluate the synergistic behavior of stem bark extract with ampicillin. To study the blocking of resistance pathways of Methicillin-Resistant Staphylococcus aureus (MRSA) western blot was performed. RESULTS: The results revealed that stem bark has significant anti-MRSA activity. The methanolic extract of stem bark in combination with ampicillin showed the highest synergistic effect (FICI value ≤ 0.237) against MRSA. Killing kinetics and membrane potential of ampicillin alone and in combination revealed an increase in the inhibitory potential of ampicillin against MRSA. Decolourization in iodometric assay confirmed the inhibition of β-lactamase, western blot results confirmed the blocking of penicillin-binding protein (PBP2a) expression with the restoration of MRSA sensitivity against β-lactams. CONCLUSION: It can be concluded that methanolic extract of Moringa oleifera stem bark has bioactive compounds and can be used as an adjuvant with antibiotics to modify the resistance of MDROs.
Keywords	Moringa oleifera ; Western blotting ; adjuvants ; ampicillin ; antibiotic resistance ; bark ; bark extracts ; decolorization ; high performance liquid chromatography ; immunomodulators ; membrane potential ; methicillin-resistant Staphylococcus aureus ; minimum inhibitory concentration ; molecular biology ; multiple drug resistance ; synergism ; therapeutics
Language	English
Dates of publication	2022-01
Size	p. 421-432.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	186544-4
ISSN	1573-4978 ; 0301-4851
ISSN (online)	1573-4978
ISSN	0301-4851
DOI	10.1007/s11033-021-06889-7
Database	NAL-Catalogue (AGRICOLA)

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