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Article ; Online: Oyster Pearl-Shaped Ternary Iron Chalcogenide, FeSe

Ray, Purbali / Pal, Sunanda / Sarkar, Abhimanyu / Sultana, Farhin / Basu, Arghyadeep / Show, Bibhutibhushan

ACS applied bio materials

2024 Volume 7, Issue 3, Page(s) 1621–1642

Abstract: Here, iron chalcogenide thin films were developed for the first time by using the less hazardous electrodeposition technique at optimized conditions on an FTO glass substrate. The chalcogenides have different surface, morphological, structural, and ... ...

Abstract	Here, iron chalcogenide thin films were developed for the first time by using the less hazardous electrodeposition technique at optimized conditions on an FTO glass substrate. The chalcogenides have different surface, morphological, structural, and optical properties, as well as an enzyme-free sensing behavior toward urea. Numerous small crystallites of about ∼20 to 25 nm for FeSe, ∼18 to 25 nm for FeTe, and ∼18 to 22 nm in diameter for FeSeTe are observed with partial agglomeration under an electron microscope, having a mixed phase of tetragonal and orthorhombic structures of FeSe, FeTe, and, FeSeTe, respectively. Profilometry, XRD, FE-SEM, HR-TEM, XPS, EDX, UV-vis spectroscopy, and FT-IR spectroscopy were used for the analysis of binary and ternary composite semiconductors, FeSe, FeTe, and FeSeTe, respectively. Electrochemical experiments were conducted with the chalcogenide thin films and urea as the analyte in phosphate-buffered media at a pH of ∼ 7.4 in the concentration range of 3-413 μM. Cyclic voltammetry was performed to determine the sensitivity of the prepared electrode at an optimized scan rate of 50 mV s
MeSH term(s)	Iron ; Urea/analysis ; Spectroscopy, Fourier Transform Infrared ; Chalcogens/chemistry
Chemical Substances	Iron (E1UOL152H7) ; Urea (8W8T17847W) ; Chalcogens
Language	English
Publishing date	2024-03-02
Publishing country	United States
Document type	Journal Article
ISSN	2576-6422
ISSN (online)	2576-6422
DOI	10.1021/acsabm.3c01086
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Article ; Online: A complex interplay between PGC-1 co-activators and mTORC1 regulates hematopoietic recovery following 5-fluorouracil treatment

Sunanda Basu

Stem Cell Research, Vol 12, Iss 1, Pp 178-

2014 Volume 193

Abstract: In vitro stimulation of HSCs with growth factors generally leads to their depletion. Understanding the molecular mechanisms underlying expansion of HSCs in vivo following myeloablation could lead to successful expansion of HSCs ex vivo for therapeutic ... ...

Abstract	In vitro stimulation of HSCs with growth factors generally leads to their depletion. Understanding the molecular mechanisms underlying expansion of HSCs in vivo following myeloablation could lead to successful expansion of HSCs ex vivo for therapeutic purposes. Current findings show that mTORC1 is activated in HSPCs following 5-fluorouracil treatment and that mTORC1 activation is dependent on mitochondrial ETC capacity of HSPCs. Moreover, expression of PGC-1 family members, proteins that regulate mitochondrial biogenesis, in HSPCs following 5-fluorouracil treatment changes; also, these proteins play a stage specific role in hematopoietic recovery. While PRC regulates HSCs' expansion during early recovery phase, PGC-1α regulates progenitor cell proliferation and recovery of hematopoiesis during later phase. During early recovery phase, PRC expression, mitochondrial activity and mTORC1 activation are relatively higher in PGC-1α−/− HSCs compared to WT HSCs, and PGC-1α−/− HSCs show greater expansion. Administration of rapamycin, but not NAC, during early recovery phase improves WT HSC numbers but decreases PGC-1α−/− HSC numbers. The current findings demonstrate that mTOR activation can increase HSC numbers provided that the energy demand created by mTOR activation is successfully met. Thus, critical tuning between mTORC1 activation and mitochondrial ETC capacity is crucial for HSC maintenance/expansion in response to mitogenic stimulation.
Keywords	Biology (General) ; QH301-705.5
Language	English
Publishing date	2014-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article ; Online: A complex interplay between PGC-1 co-activators and mTORC1 regulates hematopoietic recovery following 5-fluorouracil treatment.

Basu, Sunanda

Stem cell research

2014 Volume 12, Issue 1, Page(s) 178–193

Abstract	In vitro stimulation of HSCs with growth factors generally leads to their depletion. Understanding the molecular mechanisms underlying expansion of HSCs in vivo following myeloablation could lead to successful expansion of HSCs ex vivo for therapeutic purposes. Current findings show that mTORC1 is activated in HSPCs following 5-fluorouracil treatment and that mTORC1 activation is dependent on mitochondrial ETC capacity of HSPCs. Moreover, expression of PGC-1 family members, proteins that regulate mitochondrial biogenesis, in HSPCs following 5-fluorouracil treatment changes; also, these proteins play a stage specific role in hematopoietic recovery. While PRC regulates HSCs' expansion during early recovery phase, PGC-1α regulates progenitor cell proliferation and recovery of hematopoiesis during later phase. During early recovery phase, PRC expression, mitochondrial activity and mTORC1 activation are relatively higher in PGC-1α(-/-) HSCs compared to WT HSCs, and PGC-1α(-/-) HSCs show greater expansion. Administration of rapamycin, but not NAC, during early recovery phase improves WT HSC numbers but decreases PGC-1α(-/-) HSC numbers. The current findings demonstrate that mTOR activation can increase HSC numbers provided that the energy demand created by mTOR activation is successfully met. Thus, critical tuning between mTORC1 activation and mitochondrial ETC capacity is crucial for HSC maintenance/expansion in response to mitogenic stimulation.
MeSH term(s)	Animals ; Antimetabolites, Antineoplastic/pharmacology ; Cell Proliferation/drug effects ; Fluorouracil/pharmacology ; Hematopoietic Stem Cells/cytology ; Hematopoietic Stem Cells/drug effects ; Hematopoietic Stem Cells/metabolism ; Mechanistic Target of Rapamycin Complex 1 ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mitochondria/metabolism ; Multiprotein Complexes/metabolism ; Reactive Oxygen Species/metabolism ; Sirolimus/pharmacology ; TOR Serine-Threonine Kinases/metabolism ; Transcription Factors/deficiency ; Transcription Factors/genetics ; Transcription Factors/metabolism
Chemical Substances	Antimetabolites, Antineoplastic ; Ddit4 protein, mouse ; Multiprotein Complexes ; Reactive Oxygen Species ; Transcription Factors ; peroxisome-proliferator-activated receptor-gamma coactivator-1 ; TOR Serine-Threonine Kinases (EC 2.7.1.1) ; Mechanistic Target of Rapamycin Complex 1 (EC 2.7.11.1) ; Fluorouracil (U3P01618RT) ; Sirolimus (W36ZG6FT64)
Language	English
Publishing date	2014-01
Publishing country	England
Document type	Journal Article
ISSN	1876-7753
ISSN (online)	1876-7753
DOI	10.1016/j.scr.2013.10.006
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Article ; Online: PP2A in the regulation of cell motility and invasion.

Basu, Sunanda

Current protein & peptide science

2010 Volume 12, Issue 1, Page(s) 3–11

Abstract: Cell motility is a very critical phenomenon that plays an important role in the development of eukaryotic organisms. One of the well studied cell motility phenomena is chemotaxis, which is described as a directional movement of cell in response to ... ...

Abstract	Cell motility is a very critical phenomenon that plays an important role in the development of eukaryotic organisms. One of the well studied cell motility phenomena is chemotaxis, which is described as a directional movement of cell in response to changes in external chemotactic gradient. Numerous studies conducted both in unicellular organism and in mammalian cells have demonstrated the importance of phosphatidylionositol-3 kinase (PI3K) in this process. In addition, it is now well established that although PI3K plays an activation role in chemotaxis, the role of phosphatases is also critical to maintain this dynamic cyclical process. Protein phosphatase 2A (PP2A) is a major serine/threonine phosphatase that is a key player in regulating PI3K signaling. PP2A is abundantly and ubiquitously expressed and has been highly conserved during the evolution of eukaryotes. PP2A is composed of three protein subunits, A, B, and C. Subunit 'A' is a 60-65 kDa structural component, 'C' is a 36-38 kDa catalytic subunit, and 'B' is a 54-130 kDa regulatory subunit. The core complex of PP2A is comprised of the A and C subunits, which are tightly associated and this dimeric core complexes with the regulatory B subunit. The B subunit determines the substrate specificity as well as the spatial and temporal functions of PP2A. PP2A plays an important role in regulating multiple signal transduction pathways, including cell-cycle regulation, cell-growth and development, cytoskeleton dynamics, and cell motility. This review focuses on the role of PP2A in regulating motility of normal and transformed cells.
MeSH term(s)	Cell Movement/physiology ; Humans ; Neoplasm Invasiveness/pathology ; Protein Phosphatase 2/metabolism ; Signal Transduction
Chemical Substances	Protein Phosphatase 2 (EC 3.1.3.16)
Language	English
Publishing date	2010-11-10
Publishing country	United Arab Emirates
Document type	Journal Article ; Review
ZDB-ID	2045662-1
ISSN	1875-5550 ; 1389-2037
ISSN (online)	1875-5550
ISSN	1389-2037
DOI	10.2174/138920311795659443
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Article ; Online: Physiological and molecular characterisation for high temperature stress in Lens culinaris.

Kumar, Jitendra / Basu, Partha Sarathi / Gupta, Sunanda / Dubey, Sonali / Sen Gupta, Debjyoti / Singh, Narendra Pratap

Functional plant biology : FPB

2020 Volume 45, Issue 4, Page(s) 474–487

Abstract: In the present study, 11 lentil (Lens culinaris Medik) genotypes including heat tolerant and heat sensitive genotypes identified after a screening of 334 accessions of lentil for traits imparting heat tolerance, were characterised based on physiological ... ...

Abstract	In the present study, 11 lentil (Lens culinaris Medik) genotypes including heat tolerant and heat sensitive genotypes identified after a screening of 334 accessions of lentil for traits imparting heat tolerance, were characterised based on physiological traits and molecular markers. Results showed a higher reduction in pollen viability among sensitive genotypes (up to 52.3%) compared with tolerant genotypes (up to 32.4%) at 43°C. Higher photosynthetic electron transport rate was observed among heat tolerant genotypes and two heat tolerant lentil genotypes, IG 4258 (0.43) and IG 3330 (0.38) were having highest Fv/Fm values. However, membrane stability was significantly higher in only one heat tolerant genotype, ILL 10712, indicating that different mechanisms are involved to control heat tolerance in lentil. The molecular characterisation of lentil genotypes with 70 polymorphic SSR and genic markers resulted into distinct clusters in accordance with their heat stress tolerance. A functional marker ISM11257 (intron spanning marker) amplifying an allele of 205bp in size was present only among heat tolerant genotypes, and could be further used in a breeding program to identify heat tolerant lentil genotypes. The findings of this study will contribute to the development of heat tolerant lentil cultivars.
Language	English
Publishing date	2020-04-15
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2071582-1
ISSN	1445-4416 ; 1445-4408
ISSN (online)	1445-4416
ISSN	1445-4408
DOI	10.1071/FP17211
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Article ; Online: Peroxisome proliferator-activated-γ coactivator-1α-mediated mitochondrial biogenesis is important for hematopoietic recovery in response to stress.

Basu, Sunanda / Broxmeyer, Hal E / Hangoc, Giao

Stem cells and development

2013 Volume 22, Issue 11, Page(s) 1678–1692

Abstract: Hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) play a vital role in replenishment of blood cells. In addition to growth factors, energy metabolism plays an important role in cellular proliferation. Oxidative phosphorylation ... ...

Abstract	Hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) play a vital role in replenishment of blood cells. In addition to growth factors, energy metabolism plays an important role in cellular proliferation. Oxidative phosphorylation that occurs in the mitochondria is the major source of ATP. In this study, we have investigated the role of peroxisome proliferator-activated-γ coactivator-1α (PGC-1α), a major regulator of mitochondrial biogenesis, in hematopoiesis. PGC-1α is expressed in HSC/HPCs. Loss of PGC-1α minimally affects basal hematopoiesis; however, it significantly impairs stress hematopoiesis. Recovery of hematopoiesis poststress involves rapid proliferation of HSC/HPCs. Growth factors stimulate HSC/HPC proliferation in a dose-dependent manner and this response is modulated by oxygen tension. Although severe hypoxic conditions inhibit HSC/HPC proliferation, mild hypoxia enhances the clonogenic potential; however, the mechanism underlying this phenomenon remains largely unknown. Our studies demonstrate that PGC-1α-mediated mitochondrial biogenesis is critical for the increased clonogenic potential of progenitors under mild hypoxia. Metabolic programming and increased glucose uptake can drive rapid progenitor cell proliferation under relatively low oxygen tension only if the HPC has the capacity to increase PGC-1α expression and mitochondrial biogenesis. Loss of PGC-1α also impairs the long-term repopulating potential of HSCs. Our findings may have therapeutic applications for rapid recovery of blood cells following myeloablation.
MeSH term(s)	Animals ; Cell Hypoxia ; Cell Proliferation ; Energy Metabolism ; Hematopoiesis ; Hematopoietic Stem Cells/physiology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mitochondria/metabolism ; Oxidative Stress ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha ; Reactive Oxygen Species/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism
Chemical Substances	Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha ; Ppargc1a protein, mouse ; Reactive Oxygen Species ; Transcription Factors
Language	English
Publishing date	2013-02-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	2142214-X
ISSN	1557-8534 ; 1547-3287
ISSN (online)	1557-8534
ISSN	1547-3287
DOI	10.1089/scd.2012.0466
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Article ; Online: Long-term effect of fertilization and manuring on soil aggregate carbon mineralization

RAJENDRA KUMAR YADAV / T J PURAKAYASTHA / NAYAN AHMED / RUMA DAS / BIDISHA CHAKRABARTY / SUNANDA BISWAS / V K SHARMA / POOJA SINGH / DAIZEE TALUKDAR / K K MOURYA / S S WALIA / ROHITASAV SINGH / V K SHUKLA / M S YADAVA / N RAVISANKAR / BASU DEVI YADAV

The Indian Journal of Agricultural Sciences, Vol 91, Iss

2021 Volume 2

Abstract: The 32 years impact of manuring and fertilization on carbon mineralization in macro- and micro-aggregates in major soil groups of India was studied. Mollisol, Inceptisol, Vertisol, and Alfisol samples were collected (0-15 cm soil depth) from the ... ...

Abstract	The 32 years impact of manuring and fertilization on carbon mineralization in macro- and micro-aggregates in major soil groups of India was studied. Mollisol, Inceptisol, Vertisol, and Alfisol samples were collected (0-15 cm soil depth) from the treatments comprising of control, 100% NPK, 50%NPK+50% N-FYM, 50%NPK+50%N-WR (Wheat residue), 50% NPK + 50% N-GM (Green manure) in the year 2015. The results showed that the mineralizable C pool was significantly higher in treatments applied with 50%NPK+50%N-GM in Mollisol and Vertisol. In Alfisol and Inceptisol, 50% NPK+50% N-FYM and 50% NPK+50% N-GM were significantly higher in both macroaggregates and micro-aggregates protected carbon. Supplementation of fertilizer N either through FYM or various green manuring crops like Sesbania (Sesbania aculeata L. in Inceptisol), greengram (Vigna radiata L. in Mollisol), sunhemp (Crotalaria juncea L. in Vertisol) and karanj (Pongamia pinnata L. in Alfisol) significantly improved mineralizable carbon pool signifying their potential contributions to nutrient cycling and thereby nutrient availability to various crops in the cropping systems in the above soils.
Keywords	Carbon mineralization ; FYM ; Macro-aggregates ; Micro-aggregates ; Soil organic matter ; Soil organic carbon ; Agriculture ; S
Subject code	550
Language	English
Publishing date	2021-04-01T00:00:00Z
Publisher	Indian Council of Agricultural Research
Document type	Article ; Online
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Article ; Online: CCR5 ligands modulate CXCL12-induced chemotaxis, adhesion, and Akt phosphorylation of human cord blood CD34+ cells.

Basu, Sunanda / Broxmeyer, Hal E

Journal of immunology (Baltimore, Md. : 1950)

2009 Volume 183, Issue 11, Page(s) 7478–7488

Abstract: CXCL12 and its receptor CXCR4 play an important role in hematopoietic stem/progenitor cell (HSPC) migration from and retention within the bone marrow. HSPCs are very selective in their chemotactic response and undergo chemotaxis only in response to ... ...

Abstract	CXCL12 and its receptor CXCR4 play an important role in hematopoietic stem/progenitor cell (HSPC) migration from and retention within the bone marrow. HSPCs are very selective in their chemotactic response and undergo chemotaxis only in response to CXCL12. In addition to CXCR4, HSPCs express receptors for various other chemokines; however, the role of these receptors is not well understood. Freshly isolated CD34(+) cells (highly enriched for HSPCs) from cord blood (CB) express low levels of CCR5; however, if the cells were washed with acidic buffer before Ab staining to remove any ligand bound to CCR5, then nearly 80% of CD34(+) CB cells were found to express CCR5 on the cell surface. Although none of the CCR5 ligands investigated in this study (CCL3, CCL4, and CCL5) induced chemotaxis, at relatively high concentrations they transiently enhanced CXCL12-mediated chemotaxis of CD34(+) CB cells. In contrast, CXCL12-mediated adhesion of cells to VCAM-1-coated surfaces was reduced if CD34(+) CB cells were pretreated with these CCR5 ligands for 15 min. The effect of these chemokines on CXCL12-mediated responses was not at the level of CXCR4 expression, but on downstream signaling pathways elicited by CXCL12. Pretreatment with CCR5 chemokines enhanced CXCL12-mediated Akt phosphorylation, but down-modulated calcium flux in CD34(+) CB cells. Modulation of CXCL12-mediated responses of CD34(+) cells by CCR5 chemokines provides a possible mechanism that underlies movement of HSPCs during inflammation.
MeSH term(s)	Antigens, CD34/biosynthesis ; Antigens, CD34/immunology ; Blotting, Western ; Cell Adhesion/physiology ; Chemokine CCL3/immunology ; Chemokine CCL3/metabolism ; Chemokine CCL4/immunology ; Chemokine CCL4/metabolism ; Chemokine CCL5/immunology ; Chemokine CCL5/metabolism ; Chemokine CXCL12/immunology ; Chemokine CXCL12/metabolism ; Chemotaxis, Leukocyte/physiology ; Fetal Blood/immunology ; Fetal Blood/metabolism ; Flow Cytometry ; Hematopoietic Stem Cells/immunology ; Hematopoietic Stem Cells/metabolism ; Humans ; Ligands ; Phosphorylation ; Proto-Oncogene Proteins c-akt/immunology ; Proto-Oncogene Proteins c-akt/metabolism ; Receptors, CCR5/immunology ; Receptors, CCR5/metabolism ; Signal Transduction/immunology
Chemical Substances	Antigens, CD34 ; Chemokine CCL3 ; Chemokine CCL4 ; Chemokine CCL5 ; Chemokine CXCL12 ; Ligands ; Receptors, CCR5 ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
Language	English
Publishing date	2009-11-16
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.0900542
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Ud II Zs.37: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 28: Show issues

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Book ; Conference proceedings: Selection of papers pres. at the Joint Workshop of the High-Latitude Plasma Structures (HLPS) Group of the Coupling, Energetics and dynamics of Atmospheric Regions (CEDAR) Program and the Global Aspects of Plasma Structures (GAPS) Project of the Working Group 3 of the Solar Terrestrial Energy Program (STEP)

Basu, Sunanda

held on June 18 - 20, 1992, Lyons, Colo

(Radio science ; 29.1994,1)

1994

Event/congress	Joint Workshop of the HLPS Group of CEDAR and GAPS (1992.06.18-20, LyonsColo.)
Series title	Radio science ; 29.1994,1
Language	English
Size	155 S
Publisher	American Geophysical Union
Publishing place	Boulder, Colo
Document type	Book ; Conference proceedings
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Article: Transforming growth factor-{beta}1 modulates responses of CD34+ cord blood cells to stromal cell-derived factor-1/CXCL12.

Basu, Sunanda / Broxmeyer, Hal E

Blood

2005 Volume 106, Issue 2, Page(s) 485–493

Abstract: Disruption of stromal cell-derived factor-1 (SDF-1/CXCL12 [CXC chemokine ligand 12]) interaction leads to mobilization of stem/progenitor cells from bone marrow to circulation. However, prolonged exposure of CD34+ cells to SDF-1 desensitizes them to SDF- ... ...

Abstract	Disruption of stromal cell-derived factor-1 (SDF-1/CXCL12 [CXC chemokine ligand 12]) interaction leads to mobilization of stem/progenitor cells from bone marrow to circulation. However, prolonged exposure of CD34+ cells to SDF-1 desensitizes them to SDF-1. So how do cells remain responsive to SDF-1 in vivo when they are continuously exposed to SDF-1? We hypothesized that one or more mechanisms mediated by cytokines exist that could modulate SDF-1 responsiveness of CD34+ cells and the desensitization process. We considered transforming growth factor-beta1 (TGF-beta1) a possible candidate, since TGF-beta1 has effects on CD34+ cells and is produced by stromal cells, which provide niches for maintenance and proliferation of stem/progenitor cells. TGF-beta1 significantly restored SDF-1-induced chemotaxis and sustained adhesion responses in cord blood CD34+ cells preexposed to SDF-1. Effects of TGF-beta1 were dependent on the dose and duration of TGF-beta1 pretreatment. Phosphorylation of extracellular signal-regulated kinase 1 (Erk1)/Erk2 was implicated in TGF-beta1 modulation of migratory and adhesion responses to SDF-1. Our results indicate that low levels of TGF-beta1 can modulate SDF-1 responsiveness of CD34+ cells and thus may facilitate SDF-1-mediated retention and nurturing of stem/progenitor cells in bone marrow.
MeSH term(s)	Actins/metabolism ; Antigens, CD34/metabolism ; Calcium/metabolism ; Cell Adhesion/drug effects ; Chemokine CXCL12 ; Chemokines, CXC/pharmacology ; Chemotaxis/drug effects ; Colony-Forming Units Assay ; Fetal Blood/cytology ; Fetal Blood/drug effects ; Fetal Blood/immunology ; Fetal Blood/physiology ; Hematopoiesis/drug effects ; Humans ; In Vitro Techniques ; Infant, Newborn ; MAP Kinase Signaling System/drug effects ; Receptors, CXCR4/metabolism ; Transforming Growth Factor beta/pharmacology ; Transforming Growth Factor beta1
Chemical Substances	Actins ; Antigens, CD34 ; CXCL12 protein, human ; Chemokine CXCL12 ; Chemokines, CXC ; Receptors, CXCR4 ; TGFB1 protein, human ; Transforming Growth Factor beta ; Transforming Growth Factor beta1 ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2005-03-29
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	80069-7
ISSN	1528-0020 ; 0006-4971
ISSN (online)	1528-0020
ISSN	0006-4971
DOI	10.1182/blood-2004-10-4145
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