LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Back to previous search Search history
Advanced search

Your last searches

  1. AU=Hill Stephen J
  2. AU="Hatayama, Sho"
  3. AU="Ruuskanen, Suvi K"
  4. AU="Kim, Song-Rae"
  5. AU="Mizia-Stec, Katarzyna"
  6. AU="Helen V. Firth"
  7. AU=Giroux Leprieur Etienne
  8. AU="Xinhui Gao"
  9. AU="Christoph Schlapbach"
  10. AU="Akbar, Shayista"
  11. AU="Butler, Eboneé N"
  12. AU="Moura-Alves, Márcio"
  13. AU="Marcet, Ismael"
  14. AU=Eichfelder Sebastian
  15. AU=Timins M E
  16. AU="Weber, Stephan"
  17. AU=Galuska David
  18. AU="Carrieri, Mariella"
  19. AU="Hafkamp, Frederique J"
  20. AU="Jessica M. Perkins"
  21. AU="Tariq, Syeda Sumayya"
  22. AU="Meehan, Rebecca"

Search results

Result 1 - 10 of total 1586

Search options

  1. Article: Investigations of the Inner Workings of T4 Polymerase: the Work of Stephen J. Benkovic

    Kresge, Nicole / Simoni, Robert D / Hill, Robert L

    Journal of biological chemistry. 2009 Oct. 16, v. 284, no. 42

    2009  

    Abstract: ... of the Associated 3' [rightward arrow] 5'-Exonuclease (Gupta, A., DeBrosse, C., and Benkovic, S. J. (1982) J. Biol ... DNA Polymerase Enzyme (Gopalakrishnan, V., and Benkovic, S. J. (1994) J. Biol. Chem. 269, 21123-21126) ...

    Abstract Template-Primer-dependent Turnover of (Sp)-dATPαS by T4 DNA Polymerase. The Stereochemistry of the Associated 3' [rightward arrow] 5'-Exonuclease (Gupta, A., DeBrosse, C., and Benkovic, S. J. (1982) J. Biol. Chem. 257, 7689-7692) Spatial Relationship between Polymerase and Exonuclease Active Sites of Phage T4 DNA Polymerase Enzyme (Gopalakrishnan, V., and Benkovic, S. J. (1994) J. Biol. Chem. 269, 21123-21126)
    Language English
    Dates of publication 2009-1016
    Size p. e17-e18.
    Publishing place American Society for Biochemistry and Molecular Biology
    Document type Article
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 4' 65/118: Show issues
    Z 6.2: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Book ; Online: Cultivating Compassion

    Shi, Juewei / Hill, Stephen / Franzway, Suzanne

    Going beyond crises

    2023  

    Keywords Buddhist life & practice ; Social impact of disasters ; Economic & financial crises & disasters ; adaptation to crises ; Buddhism ; community resilience ; Compassion ; crises ; Cultivating ; Franzway ; Going ; Hill ; Juewei ; Lucy ; Melville ; Stephen ; Suzanne
    Language English
    Size 1 electronic resource (338 pages)
    Publisher Peter Lang International Academic Publishers
    Publishing place Bern
    Document type Book ; Online
    Note English
    HBZ-ID HT030648035
    ISBN 9781803741932 ; 1803741937
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Kinetic analysis of fluorescent ligand binding to cell surface receptors: Insights into conformational changes and allosterism in living cells.

    Hill, Stephen J / Kilpatrick, Laura E

    British journal of pharmacology

    2023  

    Abstract: Equilibrium binding assays are one of the mainstays of current drug discovery efforts to evaluate the interaction of drugs with receptors in membranes and intact cells. However, in recent years, there has been increased focus on the kinetics of the drug- ... ...

    Abstract Equilibrium binding assays are one of the mainstays of current drug discovery efforts to evaluate the interaction of drugs with receptors in membranes and intact cells. However, in recent years, there has been increased focus on the kinetics of the drug-receptor interaction to gain insight into the lifetime of drug-receptor complexes and the rate of association of a ligand with its receptor. Furthermore, drugs that act on topically distinct sites (allosteric) from those occupied by the endogenous ligand (orthosteric site) can induce conformational changes in the orthosteric binding site leading to changes in the association and/or dissociation rate constants of orthosteric ligands. Conformational changes in the orthosteric ligand binding site can also be induced through interaction with neighbouring accessory proteins and receptor homodimerisation and heterodimerisation. In this review, we provide an overview of the use of fluorescent ligand technologies to interrogate ligand-receptor kinetics in living cells and the novel insights that they can provide into the conformational changes induced by drugs acting on a variety of cell surface receptors including G protein-coupled receptors (GPCRs), receptor tyrosine kinases (RTKs) and cytokine receptors.
    Language English
    Publishing date 2023-06-29
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 80081-8
    ISSN 1476-5381 ; 0007-1188
    ISSN (online) 1476-5381
    ISSN 0007-1188
    DOI 10.1111/bph.16185
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.146: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Identification of an X-Band Clock Transition in Cp'

    Smith, Patrick W / Hrubý, Jakub / Evans, William J / Hill, Stephen / Minasian, Stefan G

    Journal of the American Chemical Society

    2024  Volume 146, Issue 9, Page(s) 5781–5785

    Abstract: Molecular qubits offer an attractive basis for quantum information processing, but challenges remain with regard to sustained coherence. Qubits based on clock transitions offer a method to improve the coherence times. We propose a general strategy for ... ...

    Abstract Molecular qubits offer an attractive basis for quantum information processing, but challenges remain with regard to sustained coherence. Qubits based on clock transitions offer a method to improve the coherence times. We propose a general strategy for identifying molecules with high-frequency clock transitions in systems where a d electron is coupled to a crystal-field singlet state of an f configuration, resulting in an
    Language English
    Publishing date 2024-02-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3155-0
    ISSN 1520-5126 ; 0002-7863
    ISSN (online) 1520-5126
    ISSN 0002-7863
    DOI 10.1021/jacs.3c12725
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 4' 55/32: Show issues
    Z 7.3: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: Kinetic analysis of endogenous β

    Cullum, Sean A / Veprintsev, Dmitry B / Hill, Stephen J

    British journal of pharmacology

    2023  Volume 180, Issue 10, Page(s) 1304–1315

    Abstract: Background and aim: Standard pharmacological analysis of agonist activity utilises measurements of receptor-mediated responses at a set time-point, or at the peak response level, to characterise ligands. However, the occurrence of non-equilibrium ... ...

    Abstract Background and aim: Standard pharmacological analysis of agonist activity utilises measurements of receptor-mediated responses at a set time-point, or at the peak response level, to characterise ligands. However, the occurrence of non-equilibrium conditions may dramatically impact the properties of the response being measured. Here we have analysed the initial kinetic phases of cAMP responses to β
    Experimental approach: The kinetics of β
    Key results: The partial agonists salbutamol and salmeterol displayed reduced relative IR
    Conclusions and implications: Kinetic analysis of real-time signalling data can provide valuable insights into the hemi-equilibria that can occur in low receptor reserve systems with agonist-antagonist interactions, due to incomplete dissociation of antagonist whilst the peak agonist response is developing.
    MeSH term(s) Humans ; Adrenergic beta-Agonists/pharmacology ; Adrenergic beta-Antagonists ; Bisoprolol ; HEK293 Cells ; Isoproterenol/pharmacology ; Kinetics ; Receptors, Adrenergic, beta-2 ; Cyclic AMP/metabolism
    Chemical Substances Adrenergic beta-Agonists ; Adrenergic beta-Antagonists ; Bisoprolol (Y41JS2NL6U) ; Isoproterenol (L628TT009W) ; Receptors, Adrenergic, beta-2 ; Cyclic AMP (E0399OZS9N)
    Language English
    Publishing date 2023-01-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80081-8
    ISSN 1476-5381 ; 0007-1188
    ISSN (online) 1476-5381
    ISSN 0007-1188
    DOI 10.1111/bph.16008
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.146: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Book: Public health and primary care

    Hill, Alison / Griffiths, Sian / Gillam, Stephen

    partners in population health

    2007  

    Author's details Alison Hill ; Siân Griffiths ; Stephen Gillam
    Keywords Public Health ; Primary Health Care ; Health Care Reform
    Language English
    Size XXI, 217 S. : Ill., graph. Darst.
    Publisher Oxford Univ. Press
    Publishing place Oxford
    Publishing country Great Britain
    Document type Book
    HBZ-ID HT014891733
    ISBN 0-19-850853-0 ; 978-0-19-850853-3
    Database Catalogue ZB MED Medicine, Health

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2007 A 3695 order for loan Magazin

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: The use of fluorescence correlation spectroscopy to characterise the molecular mobility of G protein-coupled receptors in membrane microdomains: an update.

    Kilpatrick, Laura E / Hill, Stephen J

    Biochemical Society transactions

    2021  Volume 49, Issue 4, Page(s) 1547–1554

    Abstract: It has become increasingly apparent that some G protein-coupled receptors (GPCRs) are not homogeneously expressed within the plasma membrane but may instead be organised within distinct signalling microdomains. These microdomains localise GPCRs in close ... ...

    Abstract It has become increasingly apparent that some G protein-coupled receptors (GPCRs) are not homogeneously expressed within the plasma membrane but may instead be organised within distinct signalling microdomains. These microdomains localise GPCRs in close proximity with other membrane proteins and intracellular signalling partners and could have profound implications for the spatial and temporal control of downstream signalling. In order to probe the molecular mechanisms that govern GPCR pharmacology within these domains, fluorescence techniques with effective single receptor sensitivity are required. Of these, fluorescence correlation spectroscopy (FCS) is a technique that meets this sensitivity threshold. This short review will provide an update of the recent uses of FCS based techniques in conjunction with GPCR subtype selective fluorescent ligands to characterise dynamic ligand-receptor interactions in whole cells and using purified GPCRs.
    Language English
    Publishing date 2021-08-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 184237-7
    ISSN 1470-8752 ; 0300-5127
    ISSN (online) 1470-8752
    ISSN 0300-5127
    DOI 10.1042/BST20201001
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 944: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Transactivation of G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs): Recent insights using luminescence and fluorescence technologies.

    Kilpatrick, Laura E / Hill, Stephen J

    Current opinion in endocrine and metabolic research

    2021  Volume 16, Page(s) 102–112

    Abstract: Alterations in signalling due to bidirectional transactivation of G protein-coupled receptor (GPCRs) and receptor tyrosine kinases (RTKs) are well established. Transactivation significantly diversifies signalling networks within a cell and has been ... ...

    Abstract Alterations in signalling due to bidirectional transactivation of G protein-coupled receptor (GPCRs) and receptor tyrosine kinases (RTKs) are well established. Transactivation significantly diversifies signalling networks within a cell and has been implicated in promoting both advantageous and disadvantageous physiological and pathophysiological outcomes, making the GPCR/RTK interactions attractive new targets for drug discovery programmes. Transactivation has been observed for a plethora of receptor pairings in multiple cell types; however, the precise molecular mechanisms and signalling effectors involved can vary with receptor pairings and cell type. This short review will discuss the recent applications of proximity-based assays, such as resonance energy transfer and fluorescence-based imaging in investigating the dynamics of GPCR/RTK complex formation, subsequent effector protein recruitment and the cellular locations of complexes in living cells.
    Language English
    Publishing date 2021-03-07
    Publishing country England
    Document type Journal Article ; Review
    ISSN 2451-9650
    ISSN (online) 2451-9650
    DOI 10.1016/j.coemr.2020.10.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article ; Online: Characterisation of tyrosine kinase inhibitor-receptor interactions at VEGFR2 using sunitinib-red and nanoBRET.

    Van Daele, Marieke / Kilpatrick, Laura E / Woolard, Jeanette / Hill, Stephen J

    Biochemical pharmacology

    2023  Volume 214, Page(s) 115672

    Abstract: Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis, proliferation and migration of vascular endothelial cells. It is well known that cardiovascular safety liability for a wide range of small molecule tyrosine kinase ... ...

    Abstract Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis, proliferation and migration of vascular endothelial cells. It is well known that cardiovascular safety liability for a wide range of small molecule tyrosine kinase inhibitors (TKIs) can result from interference with the VEGFR2 signalling system. In this study we have developed a ligand-binding assay using a fluorescent analogue of sunitinib (sunitinib-red) and full length VEGFR2 tagged on its C-terminus with the bioluminescent protein nanoluciferase to monitor ligand-binding to VEGFR2 using bioluminescence resonance energy transfer (BRET). This NanoBRET assay is a proximity-based assay (requiring the fluorescent and bioluminescent components to be within 10 nm of each other) that can monitor the binding of ligands to the kinase domain of VEGFR2. Sunitinib-red was not membrane permeable but was able to monitor the binding affinity and kinetics of a range of TKIs in cell lysates. Kinetic studies showed that sunitinib-red bound rapidly to VEGFR2 at 25 °C and that cediranib had slower binding kinetics with an average residence time of 111 min. Comparison between the log K
    MeSH term(s) Sunitinib ; Vascular Endothelial Growth Factor A/metabolism ; Tyrosine Kinase Inhibitors ; Endothelial Cells/metabolism ; Ligands ; Kinetics ; Protein Kinase Inhibitors/pharmacology ; Vascular Endothelial Growth Factor Receptor-2/metabolism
    Chemical Substances Sunitinib (V99T50803M) ; Vascular Endothelial Growth Factor A ; Tyrosine Kinase Inhibitors ; Ligands ; Protein Kinase Inhibitors ; Vascular Endothelial Growth Factor Receptor-2 (EC 2.7.10.1)
    Language English
    Publishing date 2023-07-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 208787-x
    ISSN 1873-2968 ; 0006-2952
    ISSN (online) 1873-2968
    ISSN 0006-2952
    DOI 10.1016/j.bcp.2023.115672
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 19: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Factors that influence food choices in secondary school canteens: a qualitative study of pupil and staff perspectives.

    Devine, Lauren D / Gallagher, Alison M / Briggs, Stephen / Hill, Alyson J

    Frontiers in public health

    2023  Volume 11, Page(s) 1227075

    Abstract: Background: Adolescence is recognised as a period of nutritional vulnerability, with evidence indicating that United Kingdom adolescents have suboptimal dietary intakes with many failing to meet dietary recommendations. Additionally, adolescence is a ... ...

    Abstract Background: Adolescence is recognised as a period of nutritional vulnerability, with evidence indicating that United Kingdom adolescents have suboptimal dietary intakes with many failing to meet dietary recommendations. Additionally, adolescence is a time of transition when they become more independent in their dietary choices and begin to develop their own sense of autonomy and are less reliant on their parent's guidance, which is reported to lead to less favourable dietary behaviours. Reducing the prevalence of poor dietary intakes and the associated negative health consequences among this population is a public health priority and schools represent an important setting to promote positive dietary behaviours. The aim of this school-based study was to explore the factors and barriers which influence food choices within the school canteen and to identify feasible strategies to promote positive dietary behaviours within this setting.
    Methods: Thirteen focus groups with 86 pupils in Year 8 (
    Results: Using the ecological framework, multiple factors were identified which influenced pupils' selection of food in the school canteen at the individual (e.g., time/convenience), social (e.g., peer influence), physical (e.g., food/beverage placement), and macro environment (e.g., food provision) level. Suggestions for improvement of food choices were also identified at each ecological level: individual (e.g., rewards), social (e.g., pupil-led initiatives), physical (e.g., labelling), and macro environment (e.g., whole-school approaches).
    Conclusion: Low-cost and non-labour intensive practical strategies could be employed, including menu and labelling strategies, placement of foods, reviewing pricing policies and whole-school initiatives in developing future dietary interventions to positively enhance adolescents' food choices in secondary schools.
    MeSH term(s) Adolescent ; Humans ; Schools ; Diet ; Educational Status ; Qualitative Research ; Focus Groups
    Language English
    Publishing date 2023-07-14
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2711781-9
    ISSN 2296-2565 ; 2296-2565
    ISSN (online) 2296-2565
    ISSN 2296-2565
    DOI 10.3389/fpubh.2023.1227075
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top