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Article ; Online: m

Tsuchiya, Kazuo / Yoshimura, Katsuhiro / Iwashita, Yuji / Inoue, Yusuke / Ohta, Tsutomu / Watanabe, Hirofumi / Yamada, Hidetaka / Kawase, Akikazu / Tanahashi, Masayuki / Ogawa, Hiroshi / Funai, Kazuhito / Shinmura, Kazuya / Suda, Takafumi / Sugimura, Haruhiko

Cancer gene therapy

2022 Volume 29, Issue 10, Page(s) 1355–1372

Abstract: The modification of ... ...

Abstract	The modification of N
MeSH term(s)	Humans ; AlkB Homolog 5, RNA Demethylase/genetics ; AlkB Homolog 5, RNA Demethylase/metabolism ; Carcinoma, Non-Small-Cell Lung/genetics ; Carcinoma, Non-Small-Cell Lung/metabolism ; Cell Proliferation/genetics ; Lung Neoplasms/pathology ; Prognosis ; RNA, Messenger/genetics ; RNA-Binding Proteins/metabolism
Chemical Substances	AlkB Homolog 5, RNA Demethylase (EC 1.14.11.-) ; ALKBH5 protein, human (EC 1.14.11.-) ; RNA, Messenger ; RNA-Binding Proteins
Language	English
Publishing date	2022-03-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1212513-1
ISSN	1476-5500 ; 0929-1903
ISSN (online)	1476-5500
ISSN	0929-1903
DOI	10.1038/s41417-022-00451-8
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Zs.A 4125: Show issues

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Article ; Online: M

Singh, Raman Deep / Hillestad, Matthew L / Livia, Christopher / Li, Mark / Alekseev, Alexey E / Witt, Tyra A / Stalboerger, Paul G / Yamada, Satsuki / Terzic, Andre / Behfar, Atta

Tissue engineering. Part A

2018 Volume 25, Issue 1-2, Page(s) 145–158

Abstract: Impact statement: The M ...

Abstract	Impact statement: The M
MeSH term(s)	Animals ; Disease Models, Animal ; Gene Transfer Techniques ; HEK293 Cells ; Humans ; Luciferases/biosynthesis ; Luciferases/genetics ; Mice ; Myocardial Infarction/drug therapy ; Myocardial Infarction/genetics ; Myocardial Infarction/metabolism ; Myocardial Infarction/pathology ; Myocytes, Cardiac/metabolism ; Myocytes, Cardiac/pathology ; RNA, Messenger/chemistry ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Messenger/pharmacology ; Swine
Chemical Substances	RNA, Messenger ; Luciferases (EC 1.13.12.-)
Language	English
Publishing date	2018-09-21
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2420582-5
ISSN	1937-335X ; 1937-3341
ISSN (online)	1937-335X
ISSN	1937-3341
DOI	10.1089/ten.TEA.2017.0445
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Zs.A 5500/A: Show issues

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Article ; Online: Photocatalytic solar hydrogen production from water on a 100-m

Nishiyama, Hiroshi / Yamada, Taro / Nakabayashi, Mamiko / Maehara, Yoshiki / Yamaguchi, Masaharu / Kuromiya, Yasuko / Nagatsuma, Yoshie / Tokudome, Hiromasa / Akiyama, Seiji / Watanabe, Tomoaki / Narushima, Ryoichi / Okunaka, Sayuri / Shibata, Naoya / Takata, Tsuyoshi / Hisatomi, Takashi / Domen, Kazunari

Nature

2021 Volume 598, Issue 7880, Page(s) 304–307

Abstract: The unprecedented impact of human activity on Earth's climate and the ongoing increase in global energy demand have made the development of carbon-neutral energy sources ever more important. Hydrogen is an attractive and versatile energy carrier (and ... ...

Abstract	The unprecedented impact of human activity on Earth's climate and the ongoing increase in global energy demand have made the development of carbon-neutral energy sources ever more important. Hydrogen is an attractive and versatile energy carrier (and important and widely used chemical) obtainable from water through photocatalysis using sunlight, and through electrolysis driven by solar or wind energy
Language	English
Publishing date	2021-08-25
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/s41586-021-03907-3
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Zs.A 26: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Maternal hypothyroidism is associated with M-opsin developmental delay.

Saito, Kazuma / Horiguchi, Kazuhiko / Yamada, Sayaka / Buyandalai, Battsetseg / Ishida, Emi / Matsumoto, Shunichi / Yoshino, Satoshi / Nakajima, Yasuyo / Yamada, Eijiro / Saito, Tsugumichi / Ozawa, Atsushi / Tajika, Yuki / Akiyama, Hideo / Yamada, Masanobu

Journal of molecular endocrinology

2022 Volume 69, Issue 3, Page(s) 391–399

Abstract: ... mice show delayed M-opsin development and expanded distribution of S-opsin on the retina ... in thyrotropin-releasing hormone knockout (TRH-/-) mice. We examined the mRNA expression and protein distribution of S/M-opsin ... in TRH+/- mice born to TRH+/- or TRH-/- dams, and conducted S/M-opsin analysis in TRH+/+ or TRH-/- mice ...

Abstract	Thyroid hormones are critical for the development of opsins involved in color vision. Hypothyroid mice show delayed M-opsin development and expanded distribution of S-opsin on the retina. However, the effects of maternal hypothyroidism on opsin development remain unknown. This study investigates the effects of congenital central hypothyroidism and maternal hypothyroidism on opsin development in thyrotropin-releasing hormone knockout (TRH-/-) mice. We examined the mRNA expression and protein distribution of S/M-opsin on postnatal days (P)12 and 17, as well as mRNA expression of type 2 and 3 iodothyronine deiodinase (DIO2 and DIO3, respectively) in the retina and type 1 iodothyronine deiodinase (DIO1) in the liver at P12 in TRH+/- mice born to TRH+/- or TRH-/- dams, and conducted S/M-opsin analysis in TRH+/+ or TRH-/- mice born to TRH+/- dams at P12, P17, and P30. M-opsin expression was lower in TRH+/- mice born to TRH-/- dams than in those born to TRH+/- dams, whereas S-opsin expression did not significantly differ between them. DIO1, DIO2, and DIO3 mRNA expression levels were not significantly different between the two groups; therefore, thyroid function in peripheral tissues in the pups was similar. S/M-opsin expression did not significantly differ between the TRH+/+ and TRH-/- mice born to TRH+/- dams on any postnatal day. These results demonstrate that maternal hypothyroidism causes M-opsin developmental delay during the early developmental stages of neonatal mice, and TRH-/- mice, a model of congenital central hypothyroidism, born to a euthyroid dam do not have delayed opsin development.
MeSH term(s)	Animals ; Congenital Hypothyroidism/genetics ; Congenital Hypothyroidism/metabolism ; Iodide Peroxidase/genetics ; Iodide Peroxidase/metabolism ; Mice ; Opsins ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Thyrotropin/metabolism ; Thyrotropin-Releasing Hormone/metabolism
Chemical Substances	Opsins ; RNA, Messenger ; Thyrotropin-Releasing Hormone (5Y5F15120W) ; Thyrotropin (9002-71-5) ; Iodide Peroxidase (EC 1.11.1.8)
Language	English
Publishing date	2022-08-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	645012-x
ISSN	1479-6813 ; 0952-5041
ISSN (online)	1479-6813
ISSN	0952-5041
DOI	10.1530/JME-22-0114
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Article ; Online: Adamantylidene Addition to M

Yamada, Michio / Abe, Tsuneyuki / Saito, Chiharu / Yamazaki, Toshiki / Sato, Satoru / Mizorogi, Naomi / Slanina, Zdenek / Uhlík, Filip / Suzuki, Mitsuaki / Maeda, Yutaka / Lian, Yongfu / Lu, Xing / Olmstead, Marilyn M / Balch, Alan L / Nagase, Shigeru / Akasaka, Takeshi

Chemistry (Weinheim an der Bergstrasse, Germany)

2017 Volume 23, Issue 27, Page(s) 6552–6561

Abstract: Additions of adamantylidene (Ad) to M ...

Abstract	Additions of adamantylidene (Ad) to M
Language	English
Publishing date	2017-05-11
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1478547-x
ISSN	1521-3765 ; 0947-6539
ISSN (online)	1521-3765
ISSN	0947-6539
DOI	10.1002/chem.201700049
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Article ; Online: TAK-071, a novel M

Sako, Yuu / Kurimoto, Emi / Mandai, Takao / Suzuki, Atsushi / Tanaka, Maiko / Suzuki, Motohisa / Shimizu, Yuji / Yamada, Masami / Kimura, Haruhide

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

2018 Volume 44, Issue 5, Page(s) 950–960

Abstract: The muscarinic M ...

Abstract	The muscarinic M
MeSH term(s)	Animals ; Cholinergic Agents/adverse effects ; Cholinergic Agents/pharmacology ; Cognitive Dysfunction/chemically induced ; Cognitive Dysfunction/drug therapy ; Diarrhea/chemically induced ; Electrophysiological Phenomena/drug effects ; Hippocampus/drug effects ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Mice, Knockout ; Muscarinic Antagonists/pharmacology ; Rats ; Rats, Long-Evans ; Rats, Sprague-Dawley ; Receptor, Muscarinic M1/drug effects ; Scopolamine/pharmacology
Chemical Substances	Cholinergic Agents ; Muscarinic Antagonists ; Receptor, Muscarinic M1 ; Scopolamine (DL48G20X8X)
Language	English
Publishing date	2018-08-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639471-1
ISSN	1740-634X ; 0893-133X
ISSN (online)	1740-634X
ISSN	0893-133X
DOI	10.1038/s41386-018-0168-8
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Zs.A 2379: Show issues

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Article ; Online: AJICAP-M: Traceless Affinity Peptide Mediated Conjugation Technology for Site-Selective Antibody-Drug Conjugate Synthesis.

Matsuda, Yutaka / Shikida, Natsuki / Hatada, Noriko / Yamada, Kei / Seki, Takuya / Nakahara, Yuichi / Endo, Yuta / Shimbo, Kazutaka / Takahashi, Kazutoshi / Nakayama, Akira / Mendelsohn, Brian A / Fujii, Tomohiro / Okuzumi, Tatsuya / Hirasawa, Shigeo

Organic letters

2024

Abstract: A traceless site-selective conjugation method, "AJICAP-M", was developed for native antibodies ... Comparative in vivo assessment demonstrated AJICAP-M's superior stability over traditional ADCs ... in site-selective ADC production. This manuscript outlines AJICAP-M's methodology and its effectiveness in ADC ...

Abstract	A traceless site-selective conjugation method, "AJICAP-M", was developed for native antibodies at sites using Fc-affinity peptides, focusing on Lys248 or Lys288. It produces antibody-drug conjugates (ADCs) with consistent drug-to-antibody ratios, enhanced stability, and simplified manufacturing. Comparative in vivo assessment demonstrated AJICAP-M's superior stability over traditional ADCs. This technology has been successfully applied to continuous-flow manufacturing, marking the first achievement in site-selective ADC production. This manuscript outlines AJICAP-M's methodology and its effectiveness in ADC production.
Language	English
Publishing date	2024-04-19
Publishing country	United States
Document type	Journal Article
ISSN	1523-7052
ISSN (online)	1523-7052
DOI	10.1021/acs.orglett.4c00878
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Article ; Online: Negative correlation between organ heteroplasmy, particularly hepatic heteroplasmy, and age at death revealed by post-mortem studies of m.3243A > G cases.

Yagi, Kunimasa / Okazaki, Satoko / Ohbatake, Azusa / Nakaya, Masako / Liu, Jianhui / Arite, Eiko / Miyamoto, Yukiko / Ito, Naoko / Nakano, Kaoru / Yamaaki, Naoto / Honoki, Hisae / Fujisaka, Shiho / Chujo, Daisuke / Tsunoda, Shin-Ichiro / Yanagimoto, Kunio / Nozue, Tsuyoshi / Yamada, Masayo / Ooe, Kotaro / Araki, Tsutomu /

Nakashima, Akikatsu / Azami, Yasushi / Sodemoto, Yukio / Tadokoro, Kenichi / Nagano, Makoto / Noguchi, Tohru / Nohara, Atushi / Origasa, Hideki / Niida, Yo / Tada, Hayato

Molecular genetics and metabolism

2023 Volume 140, Issue 3, Page(s) 107691

Abstract: Mitochondrial DNA m.3243A > G mutation causes mitochondrial encephalopathy, lactic acidosis, and ... associations between m.3243A > G organ heteroplasmy and clinical phenotypes, including the age at death ... to juvenile-onset (any m.3243A > G-related symptoms appeared before 20) and stroke-like episodes. Multiple ...

Abstract	Mitochondrial DNA m.3243A > G mutation causes mitochondrial encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) and its associated multi-organ disorders, including diabetes. To clarify associations between m.3243A > G organ heteroplasmy and clinical phenotypes, including the age at death, we combined genetic and pathological examinations from seven unreported and 36 literature cases of autopsied subjects. Clinical characteristics of subjects were as follows: male, 13; female, 28; unknown, 2; the age at death, 36.9 ± 20.2 [4-82] years; BMI, 16.0 ± 2.9 [13.0-22.3]; diabetes, N = 21 (49%), diabetes onset age 38.6 ± 14.2 years; deafness, N = 27 (63%); stroke-like episodes (StLEp), N = 25 (58%); congestive heart failure (CHF), N = 15 (35%); CHF onset age, 51.3 ± 14.5 years. Causes of death (N = 32) were as follows: cardiac, N = 13 (41%); infection, N = 8 (25%); StLEp, N = 4 (13%); gastrointestinal, N = 4 (13%); renal, N = 2 (6%); hepatic, N = 1 (2%). High and low heteroplasmies were confirmed in non-regenerative and regenerative organs, respectively. Heteroplasmy of the liver, spleen, leukocytes, and kidney for all subjects was significantly associated with the age at death. Furthermore, the age at death was related to juvenile-onset (any m.3243A > G-related symptoms appeared before 20) and stroke-like episodes. Multiple linear regression analysis with the age at death as an objective variable showed the significant contribution of liver heteroplasty and juvenile-onset to the age at death. m.3243A > G organ heteroplasmy levels, particularly hepatic heteroplasmy, are significantly associated with the age at death in deceased cases.
MeSH term(s)	Humans ; Male ; Female ; Adult ; Middle Aged ; Aged ; Child, Preschool ; Child ; Adolescent ; Young Adult ; Aged, 80 and over ; Heteroplasmy ; DNA, Mitochondrial/genetics ; Mutation ; Diabetes Mellitus ; Stroke/complications ; Liver/pathology ; MELAS Syndrome/genetics
Chemical Substances	DNA, Mitochondrial
Language	English
Publishing date	2023-08-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1418518-0
ISSN	1096-7206 ; 1096-7192
ISSN (online)	1096-7206
ISSN	1096-7192
DOI	10.1016/j.ymgme.2023.107691
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Zs.A 617: Show issues

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Article ; Online: A prospective cohort study of newborns born to mothers with serum Toxoplasma gondii immunoglobulin M positivity during pregnancy.

Hijikata, Midori / Morioka, Ichiro / Okahashi, Aya / Nagano, Nobuhiko / Kawakami, Kaori / Komatsu, Atsushi / Kawana, Kei / Ohyama, Shohei / Fujioka, Kazumichi / Tanimura, Kenji / Deguchi, Masashi / Sasai, Miwa / Yamamoto, Masahiro / Yamada, Hideto

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy

2021 Volume 28, Issue 4, Page(s) 486–491

Abstract: ... gondii) immunoglobulin (Ig) M-positive mothers and to clarify the incidences of serum T. gondii IgM or ...

Abstract	Introduction: The aims were to investigate the clinical characteristics of Toxoplasma gondii (T. gondii) immunoglobulin (Ig) M-positive mothers and to clarify the incidences of serum T. gondii IgM or blood T. gondii DNA positivity in newborns born to the mothers and the actual congenital T. gondii infection. Methods: Mothers with T. gondii IgM positivity and newborns born to the mothers from 2013 to 2020 were prospectively investigated. Serum T. gondii IgG and IgM were measured by enzyme-linked immunosorbent assay. Blood T. gondii DNA was detected by semi-nested polymerase chain reaction. Congenital T. gondii infection was diagnosed based on clinical characteristic manifestations with serum T. gondii IgG positivity at any age or T. gondii IgG positivity after 12 months of age. Results: Among 71 T. gondii IgM-positive mothers, including one with triplets, 41% had low T. gondii IgG avidity index and 73% received maternal therapy. Among 73 newborns who were examined for serum T. gondii IgG and IgM at birth, none had clinical manifestations, and one (1.4%) had T. gondii IgM positivity. Among 32 newborns who were examined for blood T. gondii DNA at birth, two (6.3%) were positive. All patients with serum T. gondii IgM or blood T. gondii DNA positivity showed T. gondii IgG negativity within 12 months of age. Conclusions: A few newborns born to T. gondii IgM-positive mothers were suspected of having congenital T. gondii infection based on serum T. gondii IgM or blood T. gondii DNA testing at birth. However, none developed congenital T. gondii infection.
MeSH term(s)	Antibodies, Protozoan ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoglobulin M ; Infant, Newborn ; Mothers ; Pregnancy ; Prospective Studies ; Toxoplasma/genetics
Chemical Substances	Antibodies, Protozoan ; Immunoglobulin M
Language	English
Publishing date	2021-12-17
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1355399-9
ISSN	1437-7780 ; 1341-321X
ISSN (online)	1437-7780
ISSN	1341-321X
DOI	10.1016/j.jiac.2021.12.005
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Zs.A 5236: Show issues

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Article ; Online: M Segment-Based Minigenome System of Severe Fever with Thrombocytopenia Syndrome Virus as a Tool for Antiviral Drug Screening.

Yamada, Hiroshi / Taniguchi, Satoshi / Shimojima, Masayuki / Tan, Long / Kimura, Miyuki / Morinaga, Yoshitomo / Fukuhara, Takasuke / Matsuura, Yoshiharu / Komeno, Takashi / Furuta, Yousuke / Saijo, Masayuki / Tani, Hideki

Viruses

2021 Volume 13, Issue 6

Abstract: Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne bunyavirus that causes severe disease in humans with case fatality rates of approximately 30%. There are few treatment options for SFTSV infection. SFTSV RNA synthesis is ...

Abstract	Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne bunyavirus that causes severe disease in humans with case fatality rates of approximately 30%. There are few treatment options for SFTSV infection. SFTSV RNA synthesis is conducted using a virus-encoded complex with RNA-dependent RNA polymerase activity that is required for viral propagation. This complex and its activities are, therefore, potential antiviral targets. A library of small molecule compounds was processed using a high-throughput screening (HTS) based on an SFTSV minigenome assay (MGA) in a 96-well microplate format to identify potential lead inhibitors of SFTSV RNA synthesis. The assay confirmed inhibitory activities of previously reported SFTSV inhibitors, favipiravir and ribavirin. A small-scale screening using MGA identified four candidate inhibitors that inhibited SFTSV minigenome activity by more than 80% while exhibiting less than 20% cell cytotoxicity with selectivity index (SI) values of more than 100. These included mycophenolate mofetil, methotrexate, clofarabine, and bleomycin. Overall, these data demonstrate that the SFTSV MGA is useful for anti-SFTSV drug development research.
MeSH term(s)	Antiviral Agents/pharmacology ; Cell Line ; Drug Evaluation, Preclinical/methods ; Genome, Viral ; HEK293 Cells ; High-Throughput Screening Assays/methods ; Humans ; Phlebovirus/drug effects ; Phlebovirus/genetics ; Severe Fever with Thrombocytopenia Syndrome
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2021-06-03
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v13061061
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