LIVIVO - Search results -

Search results

Result 1 - 10 of total 33

Search options

Article: Piceid Octanoate Protects Retinal Cells against Oxidative Damage by Regulating the Sirtuin 1/Poly-ADP-Ribose Polymerase 1 Axis In Vitro and in rd10 Mice.

Moshtaghion, Seyed Mohamadmehdi / Caballano-Infantes, Estefanía / Plaza Reyes, Álvaro / Valdés-Sánchez, Lourdes / Fernández, Patricia Gallego / de la Cerda, Berta / Riga, Maurizio S / Álvarez-Dolado, Manuel / Peñalver, Pablo / Morales, Juan C / Díaz-Corrales, Francisco J

Antioxidants (Basel, Switzerland)

2024 Volume 13, Issue 2

Abstract: Retinitis pigmentosa is a common cause of inherited blindness in adults, which in many cases is associated with an increase in the formation of reactive oxygen species (ROS) that induces DNA damage, triggering Poly-ADP-Ribose Polymerase 1 (PARP1) ... ...

Abstract	Retinitis pigmentosa is a common cause of inherited blindness in adults, which in many cases is associated with an increase in the formation of reactive oxygen species (ROS) that induces DNA damage, triggering Poly-ADP-Ribose Polymerase 1 (PARP1) activation and leading to parthanatos-mediated cell death. Previous studies have shown that resveratrol (RSV) is a promising molecule that can mitigate PARP1 overactivity, but its low bioavailability is a limitation for medical use. This study examined the impact of a synthesized new acylated RSV prodrug, piceid octanoate (PIC-OCT), in the 661W cell line against H
Language	English
Publishing date	2024-02-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox13020201
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: A 32-Channel Time-Multiplexed Artifact-Aware Neural Recording System.

Perez-Prieto, Norberto / Rodriguez-Vazquez, Angel / Alvarez-Dolado, Manuel / Delgado-Restituto, Manuel

IEEE transactions on biomedical circuits and systems

2021 Volume 15, Issue 5, Page(s) 960–977

Abstract: This paper presents a low-power, low-noise microsystem for the recording of neural local field potentials or intracranial electroencephalographic signals. It features 32 time-multiplexed channels at the electrode interface and offers the possibility to ... ...

Abstract	This paper presents a low-power, low-noise microsystem for the recording of neural local field potentials or intracranial electroencephalographic signals. It features 32 time-multiplexed channels at the electrode interface and offers the possibility to spatially delta encode data to take advantage of the large correlation of signals captured from nearby channels. The circuit also implements a mixed-signal voltage-triggered auto-ranging algorithm which allows to attenuate large interferers in digital domain while preserving neural information. This effectively increases the system dynamic range and avoids the onset of saturation. A prototype, fabricated in a standard 180 nm CMOS process, has been experimentally verified in-vitro with cellular cultures of primary cortical neurons from mice. The system shows an integrated input-referred noise in the 0.5-200 Hz band of 1.4 μV
MeSH term(s)	Algorithms ; Amplifiers, Electronic ; Animals ; Artifacts ; Electric Power Supplies ; Electrodes ; Equipment Design ; Mice ; Neurons ; Signal Processing, Computer-Assisted
Language	English
Publishing date	2021-12-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-9990
ISSN (online)	1940-9990
DOI	10.1109/TBCAS.2021.3108725
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Specific contribution of Reelin expressed by Cajal-Retzius cells or GABAergic interneurons to cortical lamination.

Vílchez-Acosta, Alba / Manso, Yasmina / Cárdenas, Adrián / Elias-Tersa, Alba / Martínez-Losa, Magdalena / Pascual, Marta / Álvarez-Dolado, Manuel / Nairn, Angus C / Borrell, Víctor / Soriano, Eduardo

Proceedings of the National Academy of Sciences of the United States of America

2022 Volume 119, Issue 37, Page(s) e2120079119

Abstract: The extracellular protein Reelin, expressed by Cajal-Retzius (CR) cells at early stages of cortical development and at late stages by GABAergic interneurons, regulates radial migration and the "inside-out" pattern of positioning. Current models of Reelin ...

Abstract	The extracellular protein Reelin, expressed by Cajal-Retzius (CR) cells at early stages of cortical development and at late stages by GABAergic interneurons, regulates radial migration and the "inside-out" pattern of positioning. Current models of Reelin functions in corticogenesis focus on early CR cell-derived Reelin in layer I. However, developmental disorders linked to Reelin deficits, such as schizophrenia and autism, are related to GABAergic interneuron-derived Reelin, although its role in migration has not been established. Here we selectively inactivated the
MeSH term(s)	Animals ; Cell Movement ; Cerebral Cortex/cytology ; Cerebral Cortex/embryology ; GABAergic Neurons/enzymology ; Hippocampus/embryology ; Hippocampus/enzymology ; Interneurons/enzymology ; Mice ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; Neurons/cytology ; Neurons/enzymology ; Reelin Protein/genetics ; Reelin Protein/metabolism
Chemical Substances	Nerve Tissue Proteins ; Reelin Protein ; Reln protein, mouse (EC 3.4.21.-)
Language	English
Publishing date	2022-09-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.2120079119
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1148: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Cryopreservation of GABAergic Neuronal Precursors for Cell-Based Therapy.

Rodríguez-Martínez, Daniel / Martínez-Losa, María Magdalena / Alvarez-Dolado, Manuel

PloS one

2017 Volume 12, Issue 1, Page(s) e0170776

Abstract: Cryopreservation protocols are essential for stem cells storage in order to apply them in the clinic. Here we describe a new standardized cryopreservation protocol for GABAergic neural precursors derived from the medial glanglionic eminence (MGE), a ... ...

Abstract	Cryopreservation protocols are essential for stem cells storage in order to apply them in the clinic. Here we describe a new standardized cryopreservation protocol for GABAergic neural precursors derived from the medial glanglionic eminence (MGE), a promising source of GABAergic neuronal progenitors for cell therapy against interneuron-related pathologies. We used 10% Me2SO as cryoprotectant and assessed the effects of cell culture amplification and cellular organization, as in toto explants, neurospheres, or individualized cells, on post-thaw cell viability and retrieval. We confirmed that in toto cryopreservation of MGE explants is an optimal preservation system to keep intact the interneuron precursor properties for cell transplantation, together with a high cell viability (>80%) and yield (>70%). Post-thaw proliferation and self-renewal of the cryopreserved precursors were tested in vitro. In addition, their migration capacity, acquisition of mature neuronal morphology, and potency to differentiate into multiple interneuron subtypes were also confirmed in vivo after transplantation. The results show that the cryopreserved precursor features remained intact and were similar to those immediately transplanted after their dissection from the MGE. We hope this protocol will facilitate the generation of biobanks to obtain a permanent and reliable source of GABAergic precursors for clinical application in cell-based therapies against interneuronopathies.
MeSH term(s)	Animals ; Cell Culture Techniques ; Cell Shape/physiology ; Cell Survival/physiology ; Cell- and Tissue-Based Therapy/methods ; Cryopreservation/methods ; Cryoprotective Agents ; GABAergic Neurons/cytology ; Interneurons/cytology ; Median Eminence/cytology ; Mice ; Neural Stem Cells/cytology
Chemical Substances	Cryoprotective Agents
Language	English
Publishing date	2017
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0170776
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: GABAergic neuronal precursor grafting: implications in brain regeneration and plasticity.

Alvarez Dolado, Manuel / Broccoli, Vania

Neural plasticity

2011 Volume 2011, Page(s) 384216

Abstract: Numerous neurological disorders are caused by a dysfunction of the GABAergic system that impairs or either stimulates its inhibitory action over its neuronal targets. Pharmacological drugs have generally been proved very effective in restoring its normal ...

Abstract	Numerous neurological disorders are caused by a dysfunction of the GABAergic system that impairs or either stimulates its inhibitory action over its neuronal targets. Pharmacological drugs have generally been proved very effective in restoring its normal function, but their lack of any sort of spatial or cell type specificity has created some limitations in their use. In the last decades, cell-based therapies using GABAergic neuronal grafts have emerged as a promising treatment, since they may restore the lost equilibrium by cellular replacement of the missing/altered inhibitory neurons or modulating the hyperactive excitatory system. In particular, the discovery that embryonic ganglionic eminence-derived GABAergic precursors are able to disperse and integrate in large areas of the host tissue after grafting has provided a strong rationale for exploiting their use for the treatment of diseased brains. GABAergic neuronal transplantation not only is efficacious to restore normal GABAergic activities but can also trigger or sustain high neuronal plasticity by promoting the general reorganization of local neuronal circuits adding new synaptic connections. These results cast new light on dynamics and plasticity of adult neuronal assemblies and their associated functions disclosing new therapeutic opportunities for the near future.
MeSH term(s)	Brain/physiology ; Humans ; Nervous System Diseases/therapy ; Neuronal Plasticity/physiology ; Neurons/physiology ; Regeneration ; Stem Cell Transplantation/methods ; gamma-Aminobutyric Acid/metabolism
Chemical Substances	gamma-Aminobutyric Acid (56-12-2)
Language	English
Publishing date	2011-06-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1454938-4
ISSN	1687-5443 ; 2090-5904 ; 0792-8483
ISSN (online)	1687-5443
ISSN	2090-5904 ; 0792-8483
DOI	10.1155/2011/384216
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3305: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Cell fusion: biological perspectives and potential for regenerative medicine.

Alvarez-Dolado, Manuel

Frontiers in bioscience : a journal and virtual library

2007 Volume 12, Page(s) 1–12

Abstract: Cell fusion has emerged as a powerful subject of debate in the last few years. Adult stem cell plasticity and the search for mechanisms to explain this process have led to the "rediscovery" of cell fusion. In nature, cell fusion is a normal process ... ...

Abstract	Cell fusion has emerged as a powerful subject of debate in the last few years. Adult stem cell plasticity and the search for mechanisms to explain this process have led to the "rediscovery" of cell fusion. In nature, cell fusion is a normal process involved in sexual reproduction, tissue formation, and immune response. The recent observation that bone marrow derived cells fuse with several cell types introduces new and provocative questions. In this review, I shall recapitulate what is known about cell fusion and discuss its more controversial aspects. I shall highlight the most exciting open questions; its biological potential; pros and cons; and their implications on stem cell plasticity, regenerative medicine, and development.
MeSH term(s)	Animals ; Cell Differentiation ; Cell Fusion ; Humans ; Regeneration ; Stem Cell Transplantation ; Stem Cells/cytology ; Stem Cells/physiology
Language	English
Publishing date	2007-01-01
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2141320-4
ISSN	1093-9946
ISSN	1093-9946
DOI	10.2741/2044
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5867: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: GABAergic Neuronal Precursor Grafting

Vania Broccoli / Manuel Alvarez Dolado

Neural Plasticity, Vol

Implications in Brain Regeneration and Plasticity

2011 Volume 2011

Keywords	Neurosciences. Biological psychiatry. Neuropsychiatry ; RC321-571 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Neurology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Language	English
Publishing date	2011-01-01T00:00:00Z
Publisher	Hindawi Publishing Corporation
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Bone marrow transplantation improves motor activity in a mouse model of ataxia.

Díaz, David / Piquer-Gil, Marina / Recio, Javier Sánchez / Martínez-Losa, María Magdalena / Alonso, José Ramón / Weruaga, Eduardo / Álvarez-Dolado, Manuel

Journal of tissue engineering and regenerative medicine

2018 Volume 12, Issue 4, Page(s) e1950–e1961

Abstract: Ataxias are locomotor disorders that can have an origin both neural and muscular, although both impairments are related. Unfortunately, ataxia has no cure, and the current therapies are aimed at motor re-education or muscular reinforcement. Nevertheless, ...

Abstract	Ataxias are locomotor disorders that can have an origin both neural and muscular, although both impairments are related. Unfortunately, ataxia has no cure, and the current therapies are aimed at motor re-education or muscular reinforcement. Nevertheless, cell therapy is becoming a promising approach to deal with incurable neural diseases, including neuromuscular ataxias. Here, we have used a model of ataxia, the Purkinje Cell Degeneration (PCD) mutant mouse, to study the effect of healthy (wild-type) bone marrow transplantation on the restoration of defective mobility. Bone marrow transplants (from both mutant and healthy donors) were performed in wild-type and PCD mice. Then, a wide battery of behavioural tests was employed to determine possible motor amelioration in mutants. Finally, cerebellum, spinal cord, and muscle were analysed to study the integration of the transplant-derived cells and the origin of the behavioural changes. Our results demonstrated that the transplant of wild-type bone marrow restores the mobility of PCD mice, increasing their capabilities of movement (52-100% of recovery), exploration (20-71% of recovery), speed (35% of recovery), and motor coordination (25% of recovery). Surprisingly, our results showed that bone marrow transplant notably improves the skeletal muscle structure, which is severely damaged in the mutants, rather than ameliorating the central nervous system. Although a multimodal effect of the transplant is not discarded, muscular improvements appear to be the basis of this motor recovery. Furthermore, the results from our study indicate that bone marrow stem cell therapy can be a safe and effective alternative for dealing with movement disorders such as ataxias.
MeSH term(s)	Allografts ; Animals ; Ataxia/genetics ; Ataxia/physiopathology ; Ataxia/therapy ; Bone Marrow Transplantation ; Disease Models, Animal ; Female ; Male ; Mice ; Mice, Mutant Strains ; Motor Activity
Language	English
Publishing date	2018-01-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-7005
ISSN (online)	1932-7005
DOI	10.1002/term.2626
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Cryopreservation of GABAergic Neuronal Precursors for Cell-Based Therapy.

Daniel Rodríguez-Martínez / María Magdalena Martínez-Losa / Manuel Alvarez-Dolado

PLoS ONE, Vol 12, Iss 1, p e

2017 Volume 0170776

Abstract	Cryopreservation protocols are essential for stem cells storage in order to apply them in the clinic. Here we describe a new standardized cryopreservation protocol for GABAergic neural precursors derived from the medial glanglionic eminence (MGE), a promising source of GABAergic neuronal progenitors for cell therapy against interneuron-related pathologies. We used 10% Me2SO as cryoprotectant and assessed the effects of cell culture amplification and cellular organization, as in toto explants, neurospheres, or individualized cells, on post-thaw cell viability and retrieval. We confirmed that in toto cryopreservation of MGE explants is an optimal preservation system to keep intact the interneuron precursor properties for cell transplantation, together with a high cell viability (>80%) and yield (>70%). Post-thaw proliferation and self-renewal of the cryopreserved precursors were tested in vitro. In addition, their migration capacity, acquisition of mature neuronal morphology, and potency to differentiate into multiple interneuron subtypes were also confirmed in vivo after transplantation. The results show that the cryopreserved precursor features remained intact and were similar to those immediately transplanted after their dissection from the MGE. We hope this protocol will facilitate the generation of biobanks to obtain a permanent and reliable source of GABAergic precursors for clinical application in cell-based therapies against interneuronopathies.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: GABAergic deficits in absence of LPA

Rosell-Valle, Cristina / Martínez-Losa, Magdalena / Matas-Rico, Elisa / Castilla-Ortega, Estela / Zambrana-Infantes, Emma / Gómez-Conde, Ana Isabel / Sánchez-Salido, Lourdes / Ladrón de Guevara-Miranda, David / Pedraza, Carmen / Serrano-Castro, Pedro Jesús / Chun, Jerold / Rodríguez de Fonseca, Fernando / Álvarez-Dolado, Manuel / Santín, Luis Javier / Estivill-Torrús, Guillermo

Brain structure & function

2021 Volume 226, Issue 5, Page(s) 1479–1495

Abstract: Defects in GABAergic function can cause anxiety- and depression-like behaviors among other neuropsychiatric disorders. Therapeutic strategies using the transplantation of GABAergic interneuron progenitors derived from the medial ganglionic eminence (MGE) ...

Abstract	Defects in GABAergic function can cause anxiety- and depression-like behaviors among other neuropsychiatric disorders. Therapeutic strategies using the transplantation of GABAergic interneuron progenitors derived from the medial ganglionic eminence (MGE) into the adult hippocampus reversed the symptomatology in multiple rodent models of interneuron-related pathologies. In turn, the lysophosphatidic acid receptor LPA
MeSH term(s)	Adaptation, Psychological ; Animals ; Anxiety ; GABAergic Neurons/metabolism ; Hippocampus/metabolism ; Interneurons/metabolism ; Mice ; Mice, Knockout ; Receptors, Lysophosphatidic Acid/genetics
Chemical Substances	Receptors, Lysophosphatidic Acid
Language	English
Publishing date	2021-04-01
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2273162-3
ISSN	1863-2661 ; 1863-2653
ISSN (online)	1863-2661
ISSN	1863-2653
DOI	10.1007/s00429-021-02261-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Ub I Zs.90: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito