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Article ; Online: Allelopathic effect of Chattonella marina var. marina (Raphidophyceae) on Gymnodinium catenatum (Dinophycea).

Fernández-Herrera, Leyberth J / Band-Schmidt, Christine J / López-Cortés, David J / Hernández-Guerrero, Claudia J / Bustillos-Guzmán, José J / Núñez-Vázquez, Erick

Harmful algae

2016 Volume 51, Page(s) 1–9

Abstract	The allelopathic effect of the raphidophyte Chattonella marina var. marina on the dinoflagellate Gymnodinium catenatum was determined. Both species are harmful algal bloom forming algae, produce toxic metabolites, and can co-exist in the environment. In general, raphidophytes tend to dominate over dinoflagellates, which may indicate an allelopathic effect of the former algae. Strains of C. marina var. marina and G. catenatum isolated from Bahía de La Paz were cultured in bi-algal cultures with and without cell contact. Additionally, cultures of G. catenatum were exposed to cell-free culture filtrates of the raphidophyte to test whether soluble allelopathic molecules are active. During late stationary phase, both species were cultivated in mixed cultures for 72h using the following cell abundance proportions: 20×10
Language	English
Publishing date	2016-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2091119-1
ISSN	1878-1470 ; 1568-9883
ISSN (online)	1878-1470
ISSN	1568-9883
DOI	10.1016/j.hal.2015.10.009
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Allelopathic effect of Chattonella marina var. marina (Raphidophyceae) on Gymnodinium catenatum (Dinophycea)

Fernández-Herrera, Leyberth J / Christine J. Band-Schmidt / Claudia J. Hernández-Guerrero / David J. López-Cortés / Erick Núñez-Vázquez / José J. Bustillos-Guzmán

Harmful algae. 2016 Jan., v. 51

2016

Abstract: The allelopathic effect of the raphidophyte Chattonella marina var. marina on the dinoflagellate ... dinoflagellates, which may indicate an allelopathic effect of the former algae. Strains of C. marina var. marina ... 20×103cellsL−1: 20×103cellsL−1 (1:1; G. catenatum: C. marina); 10×103cellsL−1: 20×103cellsL−1 (1:2), and ...

Abstract	The allelopathic effect of the raphidophyte Chattonella marina var. marina on the dinoflagellate Gymnodinium catenatum was determined. Both species are harmful algal bloom forming algae, produce toxic metabolites, and can co-exist in the environment. In general, raphidophytes tend to dominate over dinoflagellates, which may indicate an allelopathic effect of the former algae. Strains of C. marina var. marina and G. catenatum isolated from Bahía de La Paz were cultured in bi-algal cultures with and without cell contact. Additionally, cultures of G. catenatum were exposed to cell-free culture filtrates of the raphidophyte to test whether soluble allelopathic molecules are active. During late stationary phase, both species were cultivated in mixed cultures for 72h using the following cell abundance proportions: 20×103cellsL−1: 20×103cellsL−1 (1:1; G. catenatum: C. marina); 10×103cellsL−1: 20×103cellsL−1 (1:2), and 20×103cellsL−1: 10×103cellsL−1 (2:1). Cells of G. catenatum were also exposed to different volumes of cell filtrates of C. marina (10, 20, and 50mL) using the same cell abundance proportions for 24h. Samples were taken daily for cell counts and microscopic observations. Growth inhibition was higher when there was cell contact between both species, however mortality of G. catenatum was also observed without direct cell contact, indicating that toxic metabolites are liberated to the culture medium. Changes in cell morphology of G. catenatum occurred in the presence of cells and filtrates of C. marina, such as loss of flagella and motility, swelling, loss of girdle and sulci, prominent nucleus, rupture of cell membrane, and cell lysis. Induction of temporary cysts was also observed. These results suggest that toxic metabolites are liberated to the medium by C. marina, affecting G. catenatum by inhibiting its growth and causing changes in its life history, providing new insights of interactions between raphidophytes and dinoflagellates that could happen in the natural environment when both species are present.
Keywords	algae ; algal blooms ; allelopathy ; cell free system ; cell membranes ; culture filtrates ; culture media ; flagellum ; growth retardation ; Gymnodinium ; life history ; metabolites ; microscopy ; mixed culture ; mortality ; Raphidophyceae ; toxicity
Language	English
Dates of publication	2016-01
Size	p. 1-9.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2091119-1
ISSN	1878-1470 ; 1568-9883
ISSN (online)	1878-1470
ISSN	1568-9883
DOI	10.1016/j.hal.2015.10.009
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Determinación de la actividad antibacterial de la esponja marina Ircinia Campana recolectada en Punta Uva Limón contra Staphylococcusaureus

Rafael Rojas Brenes / Henry Borbón Alpízar / Vanessa Bagnarello Madrigal / Rigoberto Víquez Portuguez / Roy Soto Fallas / Ilena Vega Guzmán / Jacqueline Herrera Núñez

Uniciencia, Vol 26, Iss 1-2, Pp 91-

2012 Volume 97

Abstract: ... de la esponja marina Ircinia campanaque habita en el sur de la costa caribeña de Costa Rica ... Las esponjas son organismos multicelulares simples, habitan en ambientes marinos desde los mares ...

Abstract	Las esponjas son organismos multicelulares simples, habitan en ambientes marinos desde los mares polares, hasta las aguas tropicales, donde son más abundantes. Están expuestas a grandes poblaciones de microorganismos, razón que explica el complejo mecanismo de defensa morfológica y celular utilizado por estos organismos para combatir los patógenos. El propósito de este estudio fue evaluar la actividad antibacteriana de la esponja marina Ircinia campanaque habita en el sur de la costa caribeña de Costa Rica contra la bacteria Gram positiva Sthapylococcus aureus. Los ejemplares de la especie Ircinia campana fueron recolectados en Punta Uva, en la provincia de Limón durante el mes de julio del 2007. La obtención del componente activo se realizó a través de una extracción con acetona para obtener el extracto crudo; posteriormente, se realizaron separaciones cromatográficas de dicho extracto utilizando fracciones 1:4 hexano:acetato de etilo. Se analizó la actividad antibacterial de las diferentes fracciones incluyendo el extracto crudo. Los resultados obtenidos muestran una zona de inhibición de 14.60 ±0.25 mm del extracto crudo y de 18.70 ±0.25mm para la fracción más activa separada por cromatografía de columna. El metabolito responsable de la actividad antibacterial de la especie analizada se logró aislar por Cromatografía Líquida de Alta Resolución (HPLC), además se realizó una caracterización preliminar de su estructura molecularmediante espectroscopia ultravioleta (UV) e infrarroja(IR).
Keywords	actividad antibiótica ; esponja ; actividad biológica ; extractos bioactivos ; Science ; Q ; Science (General) ; Q1-390
Language	Spanish
Publishing date	2012-01-01T00:00:00Z
Publisher	Universidad Nacional, Costa Rica
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Thalassia testudinum, una planta marina con potencialidades de uso terapéutico Thalassia testudinum, a sea plant with great therapeutical potentialities

Eliane de la Torre Núñez / Idania Rodeiro Guerra / Roberto Menéndez Soto del Valle / Dalmis Pérez Carrasco

Revista Cubana de Plantas Medicinales, Vol 17, Iss 3, Pp 288-

2012 Volume 296

Abstract: ... marina Thalassia testudinum a partir de una revisión de la información existente y los principales ... de estructuras químicas novedosas con efectos farmacológicos sui generis, tal es el caso de la fenograma marina Thalassia ... resultados de las investigaciones realizadas sobre este organismo marino. Desarrollo: los productos naturales ...

Abstract	Introducción: las plantas medicinales son todas aquellas que contienen un principio activo o más, los cuales, administrados con la dosis adecuada, producen un efecto curativo a las enfermedades del hombre y los animales. El hecho de contener más de un principio activo hace que una planta medicinal pueda servir para tratar diferentes afecciones o trastornos. Objetivo: analizar los beneficios y las potencialidades terapéuticas de la planta marina Thalassia testudinum a partir de una revisión de la información existente y los principales resultados de las investigaciones realizadas sobre este organismo marino. Desarrollo: los productos naturales con propiedades terapéuticas han sido muy utilizados en la medicina tradicional. Indudablemente, los extractos de plantas de origen terrestre han representado hasta el presente una de las fuentes de mayor interés para la obtención de fitofármacos. Sin embargo, una fuente útil aún no explorada lo suficiente son los organismos marinos, que al evolucionar y vivir en condiciones ecológicas únicas y como resultado de su gran diversidad taxonómica en relación con la de los organismos terrestres, pueden sintetizar moléculas que no tienen equivalencia con las encontradas hasta el presente en estos últimos. Eso puede derivar en la obtención de estructuras químicas novedosas con efectos farmacológicos sui generis, tal es el caso de la fenograma marina Thalassia testudinum. De esta planta se ha obtenido un extracto rico en estructuras polifenólicas con potencialidades de uso terapéutico asociadas a la gran variedad de efectos beneficiosos que se le han descrito en los estudios realizados hasta el momento, como son sus propiedades antiinflamatorias, citoprotectoras, antioxidantes y neuroprotectoras, lo cual hace de este nuevo producto en desarrollo un posible fitofármaco. Conclusiones: los organismos marinos constituyen una fuente novedosa en la obtención de nuevos fitofármacos y suplementos nutricionales con una gran utilidad en la salud y la alimentación del hombre. Introduction: medicinal plants are those which contain one or more active principles that have a curative effect upon some illnesses which affect men and animals, whenever it is adequately administered. When one plant contains more than one active principle, it can be used to treat different ailments or body disorders. Objective: to analyze the benefits and therapeutic potentialities of the sea plant Thalassia testudinum based on the existent information and in the main results of research works about this sea organism. Development: natural products with therapeutic properties have been widely used in traditional medicine. Undoubtedly, the extracts from ground origin trees have so far represented one of the most interesting sources for phytodrugs. Nevertheless, sea organisms are a useful source which has not been fully explored. As they live and evolve in unique ecologic conditions, and as a result of their great taxonomic diversity in relation to that of ground organisms, they can synthesize molecules that are not equivalent to those of ground organisms found until now. This can derive in obtaining new chemical structures with sui generis pharmacological effects as in the case of the marine phenogram Thalassia testudinum. From this plant, poliphelonic structure-rich extract has been obtained, which show therapeutic potentialities associated to the great variety of beneficial effects that have been described in the studies such as anti-inflammatory, cytoprotective, antioxidative and neuroprotective properties. All the aforementioned turns this new developing product into a possible phytodrug. Conclusions: the sea organisms represent a new source to obtain new phytodrugs and nutritional supplements of great use in human health and nutrition.
Keywords	fitofármacos ; Thalassia testudinum ; propiedades terapéuticas ; phytodrug ; therapeutic properties ; Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Language	Spanish
Publishing date	2012-09-01T00:00:00Z
Publisher	ECIMED
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Obtención de una mezcla de proteínas con actividad antitumoral a partir de una bacteria marina

Vivian Almazán / Roberto Núñez / Mario Villaverde / Rita M. Pérez

Revista CENIC. Ciencias Biológicas, Vol 37, Iss 1, Pp 54-

2006 Volume 57

Abstract: Las sustancias de origen marino han sido utilizadas por el hombre desde hace millones de años ... de Microbiología Aplicada del Centro de Bioproductos Marinos se aisló una cepa de origen marino con actividad antitumoral ...

Abstract	Las sustancias de origen marino han sido utilizadas por el hombre desde hace millones de años, sin embargo, su aislamiento, caracterizaci ón química y farmacológica, así como, el interés clínico por ellas han sidos estudiados hace tan solo cinco décadas. Cuba ha incrementado gradualmente las investigaciones relacionadas con la búsqueda de compuestos con actividad antitumoral. En el Departamento de Microbiología Aplicada del Centro de Bioproductos Marinos se aisló una cepa de origen marino con actividad antitumoral con amplias posibilidades para la preservación de la salud humana. El objetivo del presente trabajo fue determinar las condiciones de cultivo de Micrococcus varians a nivel de fermentador, separar y purificar la mezcla de compuestos proteicos producidos y evaluar su posible actividad antitumoral frente a diferentes tumores malignos. La purificación de la mezcla de compuestos con actividad antitumoral producida por dicha cepa, se llevó a cabo por precipitación con (NH4)2SO4 70 % y diálisis. El criterio de pureza se corrobor ó por HPLC, cromatografía de intercambio aniónico y electroforesis en gel de poliacrilamida (8 a 25 %). La actividad antitumoral de la mezcla de compuestos arrojó un índice de sobrevida de un 77,8 % frente a sarcoma 180 y un 37,3 % frente a sarcoma 37.
Keywords	Microbiology ; QR1-502 ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2006-01-01T00:00:00Z
Publisher	Centro Nacional de Investigaciones Científicas
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book: Marina Núñez

Núñez del Prado, Marina / Villarroel Claure, Rigoberto

(Biblioteca de arte y cultura : Serie Escultores ; 15 ; Serie Arte y artistas)

1962

Author's details	Rigoberto Villarroel C
Series title	Biblioteca de arte y cultura : Serie Escultores ; 15 Serie Arte y artistas
Language	Spanish
Size	[23] Bl, zahlr. Ill
Publisher	Dirección Nacional de Informaciones de la Presidencia de la República
Publishing place	La Paz
Document type	Book
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: Escenarios relacionados con la intrusión marina. Caso de estudio Cuenca Artemisa – Quivicán, Provincia Habana. La Habana – Cuba

Manuel Núñez Lafitte

Espacio y Desarrollo, Vol 0, Iss 20, Pp 109-

2008 Volume 116

Abstract: ... expressed in the longitude of the marine intrusion wedge, taking as contour conditions the variable ...

Abstract	Sea water intrusion is the process by which the sea water in an aquifer displaces the sweet water as consequence of a sensitive reduction of the hydraulic head, either for natural actions or for anthropic activity Sea water intrusion takes place in the coastal aquifers mainly independently to the predominant lithology. In this work the scenario analysis is approached by studying the effects that sea water intrusion in dry years would produce to the supply of underground water to the City of Havana and Havana Province, when there would be high exploitation of the aquifers without maintenance of recharge works such as in South Dike. There would be a cartography of the scenario in danger to show the presage of the penetration expressed in the longitude of the marine intrusion wedge, taking as contour conditions the variable that may be unfavorable for their temporal variation, such as precipitation and depression. The thickness of the sheet of sweet water is considered constant.
Keywords	Acuífero ; Intrusión marina ; Agua subterránea ; Geography. Anthropology. Recreation ; G ; Geography (General) ; G1-922
Subject code	333
Language	Spanish
Publishing date	2008-04-01T00:00:00Z
Publisher	Pontificia Universidad Católica del Perú
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Co-ocurrencia de Chattonella marina y Gymnodinium catenatum en la Bahía de La Paz, Golfo de California (primavera 2009) Co-ocurrence of Chattonella marina and Gymnodinium catenatum in Bahía de La Paz, Gulf of California (Spring 2009)

David Javier López-Cortés / Christine Johanna Band-Schmidt / Ismael Gárate-Lizárraga / José Jesús Bustillos-Guzmán / Francisco Eduardo Hernández-Sandoval / Erick Julian Núñez-Vázquez

Hidrobiológica , Vol 21, Iss 2, Pp 185-

2011 Volume 196

Abstract: ... dos especies: Chattonella marina (Subrahmanyan) Y. Hara et Chihara y Gymnodinium catenatum Graham ... Chattonella marina fue la especie más abundante con densidades de 14 × 10³ a 36 × 10³ cél. L-1 a nivel sub ... were identified: Chattonella marina (Subrahmanyan) Y. Hara et Chihara and Gymnodinium catenatum Graham ...

Abstract	Durante el período del 24 de marzo al 14 de mayo de 2009 en la Bahía de La Paz, se obtuvieron muestras de agua y de red para análisis de fitoplancton. Una alícuota se fijó con una solución de Lugol para la identificación y cuantificación, la de red se utilizó para la identificación in vivo. Semanalmente se midió la temperatura in situ, se determinaron nutrientes inorgánicos y clorofila a en tres estaciones. Se identificaron dos especies: Chattonella marina (Subrahmanyan) Y. Hara et Chihara y Gymnodinium catenatum Graham. Chattonella marina fue la especie más abundante con densidades de 14 × 10³ a 36 × 10³ cél. L-1 a nivel sub-superficial. Gymnodinium catenatum se registró con densidades de 1 × 10³ a 19 × 10³ cél. L-1. La temperatura del agua fue homogénea desde la superficie hasta los 20 m de profundidad, con un intervalo de 19.5 a 22.0 °C y de 20.0 a 21.5 °C a finales de marzo y abril respectivamente. En la primera mitad de mayo se inició la estratificación, con una diferencia térmica de 5.0 °C entre la superficie y los 20 m. Los nitratos variaron de 0.5 a 11.0 μM y los ortofosfatos de 0.4 a 2.6 μM. La clorofila a en marzo presentó un valor en superficie de 7.5 mg m-3 y a 20 m de 3.5 mg m-3, en mayo varió de 2.0 a 7.0 mg m-3. Se concluye que la aparición de las dos especies observadas en este estudio se asocia a la disminución de los procesos de mezcla en esta bahía. During the period of 24 March to 14 May 2009, water and net samples were obtained for phytoplankton analyses; one aliquot was fixed in Lugol's solution for species quantification and identification. Net samples were used for the identification of live phytoplankton species. Additionally, temperature in situ, chlorophyll a and inorganic nutrients were measured weekly in three stations. Two species were identified: Chattonella marina (Subrahmanyan) Y. Hara et Chihara and Gymnodinium catenatum Graham. Chattonella marina was the most abundant species with a density at subsurface level of 14 × 10³ to 36 × 10³ cell L-1. Gymnodinium catenatum had densities were of 1 × 10³ to 19 × 10³ cell L-1. Water column temperature was quasi homogeneous from surface to 20 m in depth, with an interval of 19.5 to 22.0 °C and 20.0 to 21.5 °C from March to April respectively. In the middle of May stratification of the water column initiated with a slight temperature difference of 5.0 °C recorded from surface to 20 m in depth. Nitrates varied from 0.5 to 11.0 μM and orthophosphates from 0.4 to 2.6 μM. Chlorophyll a showed a value at surface level of 7.5 mg m-3 and 3.5 mg m-3 at 20 m in March; in May chlorophyll a values ranged between 2.0 to 7.0 mg m-3. We conclude that the presence of these two species during this study period was associated to the relaxation of the mixing processes in the zone.
Keywords	Chatonella marina ; Gymnodinium catenatum ; Bahía de La Paz ; Golfo de California ; fitoplancton marino ; Bahía of La Paz ; Gulf of California ; marine phytoplankton ; Biology (General) ; QH301-705.5 ; Science ; Q ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
Language	Spanish
Publishing date	2011-08-01T00:00:00Z
Publisher	UAM, Unidad Iztapalapa
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Hepatitis C and heart transplantation: An update.

Nunez, Marina / Kelkar, Anita A

Clinical transplantation

2023 Volume 37, Issue 10, Page(s) e15111

Abstract: There are limited data regarding heart transplantation in the setting of hepatitis C virus (HCV) infection in either recipients or donors, as the practice was infrequent, given concerns of worse post-transplant outcomes. This changed dramatically after ... ...

Abstract	There are limited data regarding heart transplantation in the setting of hepatitis C virus (HCV) infection in either recipients or donors, as the practice was infrequent, given concerns of worse post-transplant outcomes. This changed dramatically after the development of highly effective HCV therapies, namely direct-acting antivirals (DAAs). Additionally, nucleic acid testing currently in use establishes more precisely the risk of HCV transmission from donors. As a result, chronic HCV infection in itself is no longer a barrier for heart transplant candidates, and the use of HCV-positive organs for HCV-infected and non-infected transplant candidates has increased dramatically. A review of the literature revealed that in the pre-DAA era, HCV seropositive heart transplant patients had a higher mortality than their seronegative counterparts. However, short-term data suggest that the differences in survival have been erased in the DAA era. Heart transplantation from HCV-viremic donors to HCV-uninfected recipients has become increasingly common as the number of deceased donors with HCV viremia has increased over the past years. Preliminary outcome reports are very encouraging, although further data are needed with regard to long-term safety. New information continues to be incorporated to optimize protocols that guide this practice.
MeSH term(s)	Humans ; Hepacivirus ; Antiviral Agents/therapeutic use ; Hepatitis C, Chronic/drug therapy ; Hepatitis C ; Tissue Donors ; Heart Transplantation/adverse effects
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2023-08-31
Publishing country	Denmark
Document type	Journal Article ; Review
ZDB-ID	639001-8
ISSN	1399-0012 ; 0902-0063
ISSN (online)	1399-0012
ISSN	0902-0063
DOI	10.1111/ctr.15111
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Article ; Online: Niche models as a tool to inform restoration and conservation strategies

Ignacio Núñez-Hidalgo / Marina Fleury / Ramiro O. Bustamante

Frontiers in Forests and Global Change, Vol

the case of Jubaea chilensis (palm, Arecaceae) and its seed disperser Octodon degus (rodent, Octodontidae)

2023 Volume 6

Abstract: IntroductionBiotic interactions are a crucial component of the plant regeneration process, which has been traditionally studied at more local scales, providing the tools for planning and decision-making. Studies showing the signature of species ... ...

Abstract	IntroductionBiotic interactions are a crucial component of the plant regeneration process, which has been traditionally studied at more local scales, providing the tools for planning and decision-making. Studies showing the signature of species interactions at coarser spatial scales contrasting with animal-plant interactions at fine scales have been scarce. This study aimed at integrating an approach, over both biogeographic and local scales, by testing two endemic species of Mediterranean central Chile: the relict and southernmost threatened Chilean palm Jubaea chilensis (Chilean palm; Molina; Baillón) and the caviomorph scatter-hoarding rodent Octodon degus (Degu; Molina), on which this palm currently relies for seed dispersal.MethodsIntegrating Geographic Information Systems and Ecological Niche Modeling, the intensity of seed-rodent interactions from a territorial perspective was evaluated in the range of the palm, at a biogeographic scale, identifying areas with greater or lesser potential for seed-rodent interactions; and in local populations, incorporating a variety of environmental factors that might affect palm regeneration.ResultsThe present results show that the rodent (Octodon degus) may play a role in Chilean palm (Jubaea chilensis) seed dispersal and seed establishment, since; Chilean palm regeneration is higher in areas where both species co-occur. At a local scale, a prominent overlap between palm seedlings and degu burrows was also found, which, allied with other abiotic variables such as altitude and topographic humidity, are crucial for successful palm regeneration.DiscussionUnderstanding the full extent of animal-plant interactions and how they are affected by habitat perturbation in a wide range will provide essential information for the design of effective conservation and management strategies, such as rewilding based on plant species.
Keywords	Jubaea chilensis ; biological interactions ; species distribution models (SDMs) ; multi-scale approach ; effective disperser ; ecological conservation ; Forestry ; SD1-669.5 ; Environmental sciences ; GE1-350
Subject code	571
Language	English
Publishing date	2023-04-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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