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Book: RNAi therapeutics 2006

Takaku, Hiroshi

2006

Author's details	eds Hiroshi Takaku
Language	English
Size	146 S. : Ill., graph. Darst.
Publisher	Transworld Research Network
Publishing place	Trivandrum
Publishing country	India
Document type	Book
HBZ-ID	HT015236183
ISBN	81-7895-245-9 ; 978-81-7895-245-1
Database	Catalogue ZB MED Medicine, Health

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Article: Recombinant baculovirus expressing the FrC-OVA protein induces protective antitumor immunity in an EG7-OVA mouse model.

Kondou, Keigo / Suzuki, Tomoyuki / Chang, Myint Oo / Takaku, Hiroshi

Journal of biological engineering

2019 Volume 13, Page(s) 77

Abstract: Background: The baculovirus (BV) : Results: We constructed an rBV expressing fragment C (FrC) of tetanus toxin containing a promiscuous MHC II-binding sequence and a p30-ovalbumin (OVA) peptide that functions in the MHC I pathway. The results showed ... ...

Abstract	Background: The baculovirus (BV) Results: We constructed an rBV expressing fragment C (FrC) of tetanus toxin containing a promiscuous MHC II-binding sequence and a p30-ovalbumin (OVA) peptide that functions in the MHC I pathway. The results showed that rBV activated the CD8 Conclusion: Our findings showed that FrC-OVA-BV (rBV) induced antitumor immunity, paving the way for its use in BV immunotherapy against malignancies.
Keywords	covid19
Language	English
Publishing date	2019-10-22
Publishing country	England
Document type	Journal Article
ZDB-ID	2391582-1
ISSN	1754-1611
ISSN	1754-1611
DOI	10.1186/s13036-019-0207-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A tumor lysate is an effective vaccine antigen for the stimulation of CD4(+) T-cell function and subsequent induction of antitumor immunity mediated by CD8(+) T cells.

Kawahara, Mamoru / Takaku, Hiroshi

Cancer biology & therapy

2015 Volume 16, Issue 11, Page(s) 1616–1625

Abstract: To develop a potent cancer vaccine, it is important to study how to prepare highly immunogenic antigens and to identify the most appropriate adjuvants for the antigens. Here we show that a tumor lysate works as an effective antigen to prime CD4(+) T-cell ...

Abstract	To develop a potent cancer vaccine, it is important to study how to prepare highly immunogenic antigens and to identify the most appropriate adjuvants for the antigens. Here we show that a tumor lysate works as an effective antigen to prime CD4(+) T-cell help when baculovirus is employed as an adjuvant. When immunized intradermally with the combination (BLP) of baculovirus, a CT26 tumor lysate, and a cytotoxic T-cell epitope peptide before a tumor challenge, 60% of mice rejected tumors. In contrast, all mice vaccinated with baculovirus plus a tumor lysate (BL) developed tumors. In addition, flow cytometry showed that tumor-specific, interferon γ-producing CD8(+) cytotoxic T lymphocytes (CTLs) were robustly activated by intradermal immunization with BLP. When BLP was administered therapeutically to tumor-bearing mice, antitumor efficacy was better compared to BL. The established tumor was completely eradicated in 50-60% of BLP-treated mice, and induction of tumor-specific CTLs was observed, suggesting that the antitumor efficacy of BLP is mediated by CD8(+) T cells. Numerous CD4(+) T cells infiltrated the tumors of BLP-treated mice, whereas the antitumor effect of BLP almost disappeared after removal of the tumor lysate from BLP or after depletion of BLP-immunized mice of CD4(+) T cells. Thus, the combination of a peptide, lysate, and baculovirus provides stronger antitumor immunity than does a peptide plus baculovirus or a lysate plus baculovirus; effectiveness of BLP is determined by functioning of CD4(+) T cells stimulated with a tumor lysate.
MeSH term(s)	Animals ; Baculoviridae/immunology ; CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; Cancer Vaccines/administration & dosage ; Cancer Vaccines/immunology ; Cell Extracts/immunology ; Cell Line, Tumor ; Cytotoxicity, Immunologic ; Epitopes, T-Lymphocyte/administration & dosage ; Epitopes, T-Lymphocyte/immunology ; Female ; Humans ; Immunization ; Injections, Intradermal ; Lymphocytes, Tumor-Infiltrating/immunology ; Mice, Inbred BALB C ; Neoplasm Transplantation ; Neoplasms/immunology ; Neoplasms/therapy
Chemical Substances	Cancer Vaccines ; Cell Extracts ; Epitopes, T-Lymphocyte
Language	English
Publishing date	2015-09-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	2146305-0
ISSN	1555-8576 ; 1538-4047
ISSN (online)	1555-8576
ISSN	1538-4047
DOI	10.1080/15384047.2015.1078027
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Article ; Online: In situ elemental analyses of living biological specimens using 'NanoSuit' and EDS methods in FE-SEM.

Takaku, Yasuharu / Takehara, Sayuri / Suzuki, Chiaki / Suzuki, Hiroshi / Shimomura, Masatsugu / Hariyama, Takahiko

Scientific reports

2020 Volume 10, Issue 1, Page(s) 14574

Abstract: Energy dispersive X-ray spectroscopy (EDS) carried out alongside scanning electron microscopy (SEM) is a common technique for elemental analysis. To investigate "wet" biological specimens, complex pre-treatments are required to stabilize them under the ... ...

Abstract	Energy dispersive X-ray spectroscopy (EDS) carried out alongside scanning electron microscopy (SEM) is a common technique for elemental analysis. To investigate "wet" biological specimens, complex pre-treatments are required to stabilize them under the high vacuum conditions of high-resolution SEM. These often produce unwanted artifacts. We have previously reported that the polymerization of natural surface substances on organisms by the electron beam of the SEM setup or by plasma irradiation causes a nano-scale layer to form-called a "NanoSuit"-that can act as a barrier and keep organisms alive and hydrated in a field-emission SEM system. In the study reported herein, we examined the suitability of the NanoSuit method for elemental analyses of biological specimens by EDS. We compared experimental results for living Drosophila larvae and Aloe arborescens specimens prepared by the NanoSuit method and by conventional fixation. The NanoSuit method allowed accurate detection of the elemental compositions at high resolution. By contrast, specimens prepared by the conventional fixation method displayed additional EDS signals corresponding to the elements in the chemicals involved in the fixation process. Our results demonstrate that the NanoSuit method is useful for studying hydrous samples via EDS and SEM, particularly in biological sciences.
MeSH term(s)	Animals ; Drosophila melanogaster/metabolism ; Drosophila melanogaster/ultrastructure ; Elements ; Larva/metabolism ; Larva/ultrastructure ; Microscopy, Electron, Scanning/methods ; Nanotechnology/methods ; Polymers/chemistry ; Spectrometry, X-Ray Emission/methods ; Vacuum
Chemical Substances	Elements ; Polymers
Language	English
Publishing date	2020-09-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-71523-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Primary Intraosseous Squamous Cell Carcinoma Arising from a Dentigerous Cyst of the Maxillary Wisdom Tooth.

Takahashi, Hikaru / Takaku, Yuichiro / Kozakai, Ayako / Otsuru, Hiroshi / Murata, Yuya / Myers, Michael W

Case reports in oncology

2020 Volume 13, Issue 2, Page(s) 611–616

Abstract: The World Health Organization defines primary intraosseous squamous cell carcinoma (PIOSCC) as a squamous cell carcinoma (SCC) arising primarily within the jaws and having no connection with the oral mucosa. Here, we report a case of PIOSCC in which it ... ...

Abstract	The World Health Organization defines primary intraosseous squamous cell carcinoma (PIOSCC) as a squamous cell carcinoma (SCC) arising primarily within the jaws and having no connection with the oral mucosa. Here, we report a case of PIOSCC in which it was difficult to differentiate the condition from pericoronitis of an impacted maxillary wisdom tooth. The patient was a 27-year-old pregnant woman with a pain in the right maxillary wisdom tooth. The pain was diagnosed as pericoronitis of the right maxillary wisdom tooth, and the tooth was extracted under local anesthesia. During extraction, soft tissue was observed in the coronal part of the tooth, and it was diagnosed as SCC arising in a dentigerous cyst. Because the tumor may still be present, surgical resection was performed under general anesthesia. There has been no recurrence or metastasis at the 1-year follow-up. This case was histopathologically considered from being a benign odontogenic tumor to a malignant tumor. However, it revealed an extensive aberrant type and invasion equivalent to SCC. Thus, the histopathological diagnosis was PIOSCC arising from a dentigerous cyst. Although advanced cases of PIOSCC have been published, diagnosis of PIOSCC in the early stages is rare. In this case, we diagnosed pericoronitis of an impacted maxillary wisdom tooth and extracted the tooth. Therefore, we discovered it accidentally. In the early stages, diagnosis can be difficult both clinically and histopathologically.
Language	English
Publishing date	2020-06-04
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2458961-5
ISSN	1662-6575
ISSN	1662-6575
DOI	10.1159/000507478
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Hydrophobic-hydrophilic crown-like structure enables aquatic insects to reside effectively beneath the water surface.

Suzuki, Chiaki / Takaku, Yasuharu / Suzuki, Hiroshi / Ishii, Daisuke / Shimozawa, Tateo / Nomura, Shuhei / Shimomura, Masatsugu / Hariyama, Takahiko

Communications biology

2021 Volume 4, Issue 1, Page(s) 708

Abstract: Various insects utilise hydrophobic biological surfaces to live on the surface of water, while other organisms possess hydrophilic properties that enable them to live within a water column. Dixidae larvae reside, without being submerged, just below the ... ...

Abstract	Various insects utilise hydrophobic biological surfaces to live on the surface of water, while other organisms possess hydrophilic properties that enable them to live within a water column. Dixidae larvae reside, without being submerged, just below the water surface. However, little is known about how these larvae live in such an ecological niche. Herein, we use larvae of Dixa longistyla (Diptera: Dixidae) as experimental specimens and reveal their characteristics. A complex crown-like structure on the abdomen consists of hydrophobic and hydrophilic elements. The combination of these contrasting features enables the larvae to maintain their position as well as to move unidirectionally. Their hydrophobic region leverages water surface tension to function as an adhesive disc. By using the resistance of water, the hydrophilic region serves as a rudder during locomotion.
MeSH term(s)	Animals ; Diptera/anatomy & histology ; Diptera/physiology ; Diptera/ultrastructure ; Ecosystem ; Hydrophobic and Hydrophilic Interactions ; Surface Properties ; Surface Tension ; Water/chemistry
Chemical Substances	Water (059QF0KO0R)
Language	English
Publishing date	2021-06-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-021-02228-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Immunohistochemical Detection of Aflatoxin in Lesions of Aflatoxin-producing Aspergillus flavus Infection.

Mori, Takeshi / Abe, Hiroshi / Yoshida, Mitsutaka / Tsukune, Yutaka / Yahata, Yuriko / Takaku, Tomoiku / Ando, Jun / Ando, Miki / Torii, Shinichi / Sasaki, Makoto

Medical mycology journal

2021 Volume 62, Issue 3, Page(s) 47–52

Abstract: Aflatoxin produced by Aspergillus flavus is known to be strongly related to liver injury (hepatocellular carcinoma) and immune system damage involving leukocytes. This toxin suppresses both the cell-mediated immune system and macrophage function, and ... ...

Abstract	Aflatoxin produced by Aspergillus flavus is known to be strongly related to liver injury (hepatocellular carcinoma) and immune system damage involving leukocytes. This toxin suppresses both the cell-mediated immune system and macrophage function, and decreases the production of complement and interferon molecules. Purpose: To evaluate the presence of aflatoxin in infectious lesions as well as how the toxin is taken up by leukocytes. Method: Pathological specimens from a patient who died from aspergillosis caused by aflatoxin-producing A. flavus were used. Anti-aflatoxin B Result: Positive reactions were detected in fungal elements and leukocytes (neutrophils and macrophages) in inflammatory lesions. Conclusion: Within the patient's body, A. flavus likely produced aflatoxin, which then was taken up by neutrophils and macrophages.These results suggest that leukocyte function and the immune mechanism are locally suppressed by aflatoxin.
MeSH term(s)	Aflatoxin B1 ; Aflatoxins ; Aspergillosis ; Aspergillus flavus ; Fungi ; Humans
Chemical Substances	Aflatoxins ; Aflatoxin B1 (9N2N2Y55MH)
Language	English
Publishing date	2021-09-01
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2628906-4
ISSN	1882-0476 ; 2186-165X ; 2185-6486 ; 0916-4804
ISSN (online)	1882-0476 ; 2186-165X
ISSN	2185-6486 ; 0916-4804
DOI	10.3314/mmj.20-00020
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Article ; Online: A 30-year record reveals re-equilibration rates of

Takata, Hyoe / Johansen, Mathew P / Kusakabe, Masashi / Ikenoue, Takahito / Yokota, Mizuro / Takaku, Hiroshi

The Science of the total environment

2019 Volume 675, Page(s) 694–704

Abstract: Concentration ratios (CRs), expressed by ... ...

Abstract	Concentration ratios (CRs), expressed by dividing
MeSH term(s)	Aquatic Organisms/metabolism ; Biota ; Cesium Radioisotopes/analysis ; Cesium Radioisotopes/metabolism ; Food Chain ; Fukushima Nuclear Accident ; Japan ; Nuclear Power Plants ; Radiation Monitoring ; Seawater ; Water Pollutants, Radioactive/analysis ; Water Pollutants, Radioactive/metabolism
Chemical Substances	Cesium Radioisotopes ; Water Pollutants, Radioactive ; Cesium-137 (4T2E65IAR7)
Language	English
Publishing date	2019-04-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2019.04.015
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Article ; Online: Recombinant baculovirus expressing the FrC-OVA protein induces protective antitumor immunity in an EG7-OVA mouse model

Keigo Kondou / Tomoyuki Suzuki / Myint Oo Chang / Hiroshi Takaku

Journal of Biological Engineering, Vol 13, Iss 1, Pp 1-

2019 Volume 8

Abstract: Abstract Background The baculovirus (BV) Autographa californica multiple nuclear polyhedrosis virus has been used in numerous protein expression systems because of its ability to infect insect cells and serves as a useful vaccination vector with several ... ...

Abstract	Abstract Background The baculovirus (BV) Autographa californica multiple nuclear polyhedrosis virus has been used in numerous protein expression systems because of its ability to infect insect cells and serves as a useful vaccination vector with several benefits, such as its low clinical risks and posttranslational modification ability. We recently reported that dendritic cells (DCs) infected with BV stimulated antitumor immunity. The recombinant BV (rBV) also strongly stimulated peptide-specific T-cells and antitumor immunity. In this study, the stimulation of an immune response against EG7-OVA tumors in mice by a recombinant baculovirus-based combination vaccine expressing fragment C-ovalbumin (FrC-OVA-BV; rBV) was evaluated. Results We constructed an rBV expressing fragment C (FrC) of tetanus toxin containing a promiscuous MHC II-binding sequence and a p30-ovalbumin (OVA) peptide that functions in the MHC I pathway. The results showed that rBV activated the CD8+ T-cell-mediated response much more efficiently than the wild-type BV (wtBV). Experiments with EG7-OVA tumor mouse models showed that rBV significantly decreased tumor volume and increased survival compared with those in the wild-type BV or FrC-OVA DNA vaccine groups. In addition, a significant antitumor effect of classic prophylactic or therapeutic vaccinations was observed for rBV against EG7-OVA-induced tumors compared with that in the controls. Conclusion Our findings showed that FrC-OVA-BV (rBV) induced antitumor immunity, paving the way for its use in BV immunotherapy against malignancies.
Keywords	Recombinant baculovirus ; Wild-type baculovirus ; Fragment C of tetanus toxin ; Ovalbumin ; T cells ; Tumor immunity ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2019-10-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Gene silencing of HIV-1 by RNA interference.

Takaku, Hiroshi

Antiviral chemistry & chemotherapy

2004 Volume 15, Issue 2, Page(s) 57–65

Abstract: Short interfering RNAs (siRNAs) are as effective at targeting and silencing genes by RNA interference (RNAi) as long double-stranded RNAs (dsRNAs). siRNAs are widely used for assessing gene function in cultured mammalian cells or early developing ... ...

Abstract	Short interfering RNAs (siRNAs) are as effective at targeting and silencing genes by RNA interference (RNAi) as long double-stranded RNAs (dsRNAs). siRNAs are widely used for assessing gene function in cultured mammalian cells or early developing vertebrate embryos. siRNAs are also promising reagents for developing gene-specific therapeutics. Specifically, the inhibition of HIV-1 replication is particularly well-suited to RNAi, as several stages of the viral life cycle and many viral and cellular genes can be targeted. The future success of this approach will depend on recent advances in siRNA-based silencing technologies.
MeSH term(s)	Animals ; Gene Expression Regulation, Viral/drug effects ; Gene Silencing ; Genetic Therapy/methods ; Genetic Vectors/genetics ; Genetic Vectors/pharmacology ; HIV Infections/genetics ; HIV Infections/therapy ; HIV-1/drug effects ; HIV-1/genetics ; Humans ; Models, Biological ; RNA Interference/physiology ; RNA, Small Interfering/biosynthesis ; RNA, Small Interfering/genetics ; RNA, Small Interfering/physiology ; Virus Replication/drug effects ; Virus Replication/genetics
Chemical Substances	RNA, Small Interfering
Language	English
Publishing date	2004-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1033586-9
ISSN	2040-2066 ; 0956-3202
ISSN (online)	2040-2066
ISSN	0956-3202
DOI	10.1177/095632020401500201
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