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  1. Article ; Online: Genetic diversity of BCoV in Brazilian cattle herds.

    de Mira Fernandes, Adeline / Brandão, Paulo E / Dos Santos Lima, Michele / de Souza Nunes Martins, Maira / da Silva, Thais G / da Silva Cardoso Pinto, Vivian / de Paula, Larissa T / Vicente, Marta Elisabete S / Okuda, Liria H / Pituco, Edviges M

    Veterinary medicine and science

    2018  

    Abstract: Bovine coronavirus (BCoV) is one of the main aetiological agents of gastroenteritis in calves, causing significant economic damage to livestock. This study aims to characterise BCoV genetically on the basis of the N gene. A total of 114 faecal samples ... ...

    Abstract Bovine coronavirus (BCoV) is one of the main aetiological agents of gastroenteritis in calves, causing significant economic damage to livestock. This study aims to characterise BCoV genetically on the basis of the N gene. A total of 114 faecal samples from beef and dairy calves with or without clinical symptoms of diarrhoea from five Brazilian states (São Paulo, Minas Gerais, Santa Catarina, Mato Grosso and Bahia) were evaluated between 2008 and 2015 by technique of Semi-nested RT-PCR for gene N and genealogical analysis. Of the 114 samples analysed, 14.91% (17/114) were positive. BCoV was detected in 22.72% (10/44) of the animals with diarrhoea and in 10% (7/70) of asymptomatic animals. BCoV was identified in calves from rural properties located in all of the regions sampled. Genealogical analysis showed that the Brazilian sequences of BCoV for the gene which codes for the N protein can be broken down into two distinct clusters, and the samples from this study were closely linked to Asian strains. These results contribute to the molecular characterization of BCoV in Brazil and are the first report of the circulation of BCoV in the states of Santa Catarina and Bahia.
    Keywords covid19
    Language English
    Publishing date 2018-04-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 2819409-3
    ISSN 2053-1095 ; 2053-1095
    ISSN (online) 2053-1095
    ISSN 2053-1095
    DOI 10.1002/vms3.102
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Detection of the emerging rotavirus G12P[8] genotype at high frequency in brazil in 2014: Successive replacement of predominant strains after vaccine introduction.

    Luchs, Adriana / Cilli, Audrey / Morillo, Simone Guadagnucci / Gregório, Debora de Souza / de Souza, Karen Aparecida Farias / Vieira, Heloísa Rosa / Fernandes, Adeline de Mira / Carmona, Rita de Cássia Compagnoli / Timenetsky, Maria do Carmo Sampaio Tavares

    Acta tropica

    2016  Volume 156, Page(s) 87–94

    Abstract: The continuum characterization of rotavirus (RVA) genotypes is essential to understand how vaccine introduction could impact virus epidemiology. In the present study, an unexpected rapid changing pattern of RVA genotypes distribution in Brazilian ... ...

    Abstract The continuum characterization of rotavirus (RVA) genotypes is essential to understand how vaccine introduction could impact virus epidemiology. In the present study, an unexpected rapid changing pattern of RVA genotypes distribution in Brazilian population during three followed seasons is described. From January/2012 to December/2014, a total of 3441 fecal specimens were collected from collaborating centers across Southern, Southeastern and Midwest of Brazil. All specimens were screened for RVA using ELISA, and genotyped by RT-PCR. Differences in proportions were tested using Chi-Squares. A p-value of less than 0.05 was considered statistically significant. RVA was detected in 19.7% (677/3441). Among RVA positive cases (n=677), a total of 652 (96.3%) samples were successfully amplified by RT-PCR. G3P[8] remained prevalent in 2012 (37.6%, 69/185) and 2013 (40.1%, 74/186) (χ(2)=0.107, p=0.743), but declined markedly in 2014 (3.5%, 10/281) (χ(2)=71.770, p=0.000). G12P[8] was second highest strain in 2012 (22.7%, 42/185), decrease rapidly in 2013 (2.7%, 5/186) (χ(2)=26.224, p=0.000) and re-emerged as the predominant genotype in 2014 (86.6%, 243/281) (χ(2)=118.299, p=0.000). From July/2014, G12P[8] was the single genotype detected in all regions studied. The sudden emergence, spread and predominance of G12P[8] strain in Brazil, raised the hypothesis of a possible G12 outbreak being in progress. Nationally, the long term decline in gastroenteritis hospitalization observed in the country after RVA vaccine introduction was confirmed. Nevertheless, the sharp increase in diarrhea hospitalization prevalence from 2013 to 2014 observed in Southern and Southeastern regions is consistent with what appears to be an outbreak of G12P[8]. Continued surveillance is needed to verify the effectiveness of the RotarixTM vaccine in Brazil together with potential emergence of unusual genotypes.
    MeSH term(s) Adolescent ; Adult ; Brazil/epidemiology ; Child ; Child, Preschool ; Diarrhea/epidemiology ; Diarrhea/prevention & control ; Diarrhea/virology ; Disease Outbreaks ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Infant ; Male ; Polymerase Chain Reaction ; Population Groups ; Prevalence ; Retrospective Studies ; Rotavirus/genetics ; Rotavirus/isolation & purification ; Rotavirus Infections/epidemiology ; Rotavirus Infections/prevention & control ; Rotavirus Infections/virology ; Seasons ; Vaccines/administration & dosage ; Young Adult
    Chemical Substances Vaccines
    Language English
    Publishing date 2016-04
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2015.12.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.136: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Detection of the emerging rotavirus G12P[8] genotype at high frequency in brazil in 2014: Successive replacement of predominant strains after vaccine introduction

    Luchs, Adriana / Adeline de Mira Fernandes / Audrey Cilli / Debora de Souza Gregório / Heloísa Rosa Vieira / Karen Aparecida Farias de Souza / Maria do Carmo Sampaio Tavares Timenetsky / Rita de Cássia Compagnoli Carmona / Simone Guadagnucci Morillo

    Acta tropica. 2016 Apr., v. 156

    2016  

    Abstract: The continuum characterization of rotavirus (RVA) genotypes is essential to understand how vaccine introduction could impact virus epidemiology. In the present study, an unexpected rapid changing pattern of RVA genotypes distribution in Brazilian ... ...

    Abstract The continuum characterization of rotavirus (RVA) genotypes is essential to understand how vaccine introduction could impact virus epidemiology. In the present study, an unexpected rapid changing pattern of RVA genotypes distribution in Brazilian population during three followed seasons is described. From January/2012 to December/2014, a total of 3441 fecal specimens were collected from collaborating centers across Southern, Southeastern and Midwest of Brazil. All specimens were screened for RVA using ELISA, and genotyped by RT-PCR. Differences in proportions were tested using Chi-Squares. A p-value of less than 0.05 was considered statistically significant. RVA was detected in 19.7% (677/3441). Among RVA positive cases (n=677), a total of 652 (96.3%) samples were successfully amplified by RT-PCR. G3P[8] remained prevalent in 2012 (37.6%, 69/185) and 2013 (40.1%, 74/186) (χ2=0.107, p=0.743), but declined markedly in 2014 (3.5%, 10/281) (χ2=71.770, p=0.000). G12P[8] was second highest strain in 2012 (22.7%, 42/185), decrease rapidly in 2013 (2.7%, 5/186) (χ2=26.224, p=0.000) and re-emerged as the predominant genotype in 2014 (86.6%, 243/281) (χ2=118.299, p=0.000). From July/2014, G12P[8] was the single genotype detected in all regions studied. The sudden emergence, spread and predominance of G12P[8] strain in Brazil, raised the hypothesis of a possible G12 outbreak being in progress. Nationally, the long term decline in gastroenteritis hospitalization observed in the country after RVA vaccine introduction was confirmed. Nevertheless, the sharp increase in diarrhea hospitalization prevalence from 2013 to 2014 observed in Southern and Southeastern regions is consistent with what appears to be an outbreak of G12P[8]. Continued surveillance is needed to verify the effectiveness of the RotarixTM vaccine in Brazil together with potential emergence of unusual genotypes.
    Keywords diarrhea ; enzyme-linked immunosorbent assay ; epidemiology ; feces ; gastroenteritis ; genotype ; genotyping ; monitoring ; reverse transcriptase polymerase chain reaction ; Rotavirus ; vaccines ; viruses ; Brazil
    Language English
    Dates of publication 2016-04
    Size p. 87-94.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2015.12.008
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 52/75: Show issues

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