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Article ; Online: Correction: A healthy lifestyle text message intervention for adolescents: protocol for the Health4Me randomized controlled trial.

Raeside, Rebecca / Spielman, Karen / Maguire, Sarah / Mihrshahi, Seema / Steinbeck, Katharine / Kang, Melissa / Laranjo, Liliana / Hyun, Karice / Redfern, Julie / Partridge, Stephanie R

BMC public health

2024 Volume 24, Issue 1, Page(s) 524

Language	English
Publishing date	2024-02-20
Publishing country	England
Document type	Published Erratum
ZDB-ID	2041338-5
ISSN	1471-2458 ; 1471-2458
ISSN (online)	1471-2458
ISSN	1471-2458
DOI	10.1186/s12889-024-17698-5
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Article ; Online: A healthy lifestyle text message intervention for adolescents: protocol for the Health4Me randomized controlled trial.

Raeside, Rebecca / Spielman, Karen / Maguire, Sarah / Mihrshahi, Seema / Steinbeck, Katharine / Kang, Melissa / Laranjo, Liliana / Hyun, Karice / Redfern, Julie / Partridge, Stephanie R

BMC public health

2022 Volume 22, Issue 1, Page(s) 1805

Abstract: Background: Adolescence presents a window of opportunity to establish good nutrition and physical activity behaviours to carry throughout the life course. Adolescents are at risk of developing cardiovascular and other chronic diseases due to poor the ... ...

Abstract	Background: Adolescence presents a window of opportunity to establish good nutrition and physical activity behaviours to carry throughout the life course. Adolescents are at risk of developing cardiovascular and other chronic diseases due to poor the complex interplay of physical and mental health lifestyle risk factors. Text messaging is adolescents main form of everyday communication and text message programs offer a potential solution for support and improvement of lifestyle health behaviours. The primary aim of this study is to determine effectiveness of the Health4Me text message program to improve adolescent's physical activity or nutrition behaviours among adolescents over 6-months, compared to usual care. Methods: Health4Me is a virtual, two-arm, single-blind randomised controlled trial, delivering a 6-month healthy lifestyle text message program with optional health counselling. Recruitment will be through digital advertising and primary care services. In total, 330 adolescents will be randomised 1:1 to intervention or control (usual care) groups. The intervention group will receive 4-5 text messages per week for 6-months. All text messages have been co-designed with adolescents. Messages promote a healthy lifestyle by providing practical information, health tips, motivation and support for behaviour change for physical activity, nutrition, mental health, body image, popular digital media and climate and planetary health. Virtual assessments will occur at baseline and 6-months assessing physical health (physical activity, nutrition, body mass index, sleep), mental health (quality of life, self-efficacy, psychological distress, anxiety, depression, eating disorder risk) and lifestyle outcomes (food insecurity and eHealth literacy). Discussion: This study will determine the effectiveness of a 6-month healthy lifestyle text message intervention to improve physical activity and nutrition outcomes in adolescents. Trial registration: Australia New Zealand Clinical Trials Registry (ANZCTR) ACTRN12622000949785 , Date registered: 05/07/2022.
MeSH term(s)	Adolescent ; Healthy Lifestyle ; Humans ; Internet ; Quality of Life ; Randomized Controlled Trials as Topic ; Single-Blind Method ; Text Messaging
Language	English
Publishing date	2022-09-23
Publishing country	England
Document type	Clinical Trial Protocol ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2041338-5
ISSN	1471-2458 ; 1471-2458
ISSN (online)	1471-2458
ISSN	1471-2458
DOI	10.1186/s12889-022-14183-9
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Article: Genetic counseling and oncology: proposed approaches for collaborative care delivery.

Powers, Jacquelyn / Spielman, Kelsey / Mueller, Rebecca / Batson, Melissa / Pundock, Stacy / Arutyunova, Anna / Symecko, Heather / Domchek, Susan

The Canadian journal of urology

2019 Volume 26, Issue 5 Suppl 2, Page(s) 57–59

Abstract: Demand for cancer genetic counseling has grown rapidly in recent years as germline genomic information has integrated into cancer care. There are currently an insufficient number of genetic counselors (GC) to address genetic testing need through ... ...

Abstract	Demand for cancer genetic counseling has grown rapidly in recent years as germline genomic information has integrated into cancer care. There are currently an insufficient number of genetic counselors (GC) to address genetic testing need through traditional pre- and post-test counseling. Alternative genetic counseling frameworks, discussed here, are under study to increase access to genetic testing while optimizing the skillsets of existent master's-trained GCs.
MeSH term(s)	Delivery of Health Care/methods ; Genetic Counseling ; Genetic Testing ; Humans ; Male ; Prostatic Neoplasms/diagnosis ; Prostatic Neoplasms/genetics
Language	English
Publishing date	2019-09-26
Publishing country	Canada
Document type	Journal Article
ZDB-ID	2064475-9
ISSN	1195-9479
ISSN	1195-9479
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Article ; Online: Clinical Impact of a Rapid Genetic Testing Model for Advanced Prostate Cancer Patients.

Breen, Kelsey E / Symecko, Heather / Spielman, Kelsey / Gebert, Rebecca / Shah, Ibrahim H / Pundock, Stacy / Batson, Melissa / Narayan, Vivek K / Stadler, Zsofia K / Autio, Karen A / Abida, Wassim / Danila, Daniel C / Scher, Howard I / Morris, Michael J / Hamilton, Jada G / Robson, Mark E / Domchek, Susan M / Carlo, Maria I

The Journal of urology

2023 Volume 209, Issue 5, Page(s) 918–927

Abstract: Purpose: Genetic testing may alter clinical management for individuals with metastatic prostate cancer by identifying additional therapies. Traditional counseling models are unlikely to enable time-sensitive therapeutic decision-making. This study aimed ...

Abstract	Purpose: Genetic testing may alter clinical management for individuals with metastatic prostate cancer by identifying additional therapies. Traditional counseling models are unlikely to enable time-sensitive therapeutic decision-making. This study aimed to determine the feasibility and clinical impact of an alternative hereditary genetic testing model. Materials and methods: As part of a multicenter, single-arm prospective trial, individuals with advanced prostate cancer were referred by their oncologist for testing of 14 genes associated with hereditary prostate cancer. Pretest education (brochure and video) was provided in the oncology clinic. Questionnaires assessing participant satisfaction with both pretest education and decision to undergo genetic testing were collected. A genetic counselor contacted participants by phone to obtain family history and discuss results. Medical records were queried to determine whether a change in clinical management was discussed. Results: Of 501 participants consented to germline analysis, 51 (10.2%) had at least 1 pathogenic/likely pathogenic variant. Change in treatment was discussed with 22/48 (45.8%) of eligible participants who tested positive. Feasibility of this model was assessed by participant satisfaction and turnaround time. Average±SD satisfaction with the pretest education (15.5±2.2, 4-20 scale) and with the decision to undergo genetic testing (17.1±2.9, 4-20 scale) were both high. Results were returned 20 days (median) after sample collection. Conclusions: Oncologist-initiated germline genetic testing in collaboration with a genetic counselor is a feasible approach to testing advanced prostate cancer patients with impactful clinical actionability. The testing model and educational material serve as resources to clinicians treating prostate cancer patients.
MeSH term(s)	Male ; Humans ; Prospective Studies ; Genetic Testing ; Prostatic Neoplasms/diagnosis ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/therapy ; Genetic Counseling ; Counseling
Language	English
Publishing date	2023-03-28
Publishing country	United States
Document type	Multicenter Study ; Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	3176-8
ISSN	1527-3792 ; 0022-5347
ISSN (online)	1527-3792
ISSN	0022-5347
DOI	10.1097/JU.0000000000003186
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Article ; Online: A proof-of-concept randomized crossover clinical trial of a first-in-class vasopressin 1a receptor antagonist for PTSD: Design, methods, and recruitment.

Difede, JoAnn / McAleavey, Andrew A / Emrich, Mariel / Jick, Adina / Ovalles, Annell / Wyka, Katarzyna / Spielman, Lisa / Olden, Megan / Peskin, Melissa / Becket-Davenport, Colleen / Rubenstein, Amy / Brownstein, Michael J / Damiano, Eve / Itzkowitz, Debra / Lu, Shi-Fang / Needell, Nancy J / Kocsis, James H / Gordon-Elliott, Janna S / Simon, Neal G

Contemporary clinical trials communications

2023 Volume 33, Page(s) 101116

Abstract: Background: Almost eight million Americans suffer from Posttraumatic Stress Disorder (PTSD). Current PTSD drug therapies rely on repurposed antidepressants and anxiolytics, which produce undesirable side effects and have recognized compliance issues. ... ...

Abstract	Background: Almost eight million Americans suffer from Posttraumatic Stress Disorder (PTSD). Current PTSD drug therapies rely on repurposed antidepressants and anxiolytics, which produce undesirable side effects and have recognized compliance issues. Vasopressin represents a promising and novel target for pharmacological intervention. Logistical issues implementing a clinical trial for a novel PTSD pharmaceutical are relatively uncharted territory as trials concerning a new agent have not been published in the past several decades. All published trials have repurposed FDA-approved psychoactive medications with known risk profiles. Our recruitment challenges are discussed in this context. Methods: An 18-week proof-of-concept randomized crossover clinical trial of a first-in-class vasopressin 1a receptor antagonist (SRX246) for PTSD was conducted. All participants received SRX246 for 8 weeks, the placebo for 8 weeks, and the drug vs. placebo arms were compared. Participants were assessed every 2 weeks for PTSD symptoms as well as other medication effects. Results were expected to provide an initial demonstration of safety and tolerability in this clinical population and potentially clinical efficacy in SRX246-treated patients measured by Clinician Administered PTSD Scale (CAPS) score changes, clinical impression, and other indices compared to placebo. The primary hypothesis was that SRX246 would result in a clinically meaningful 10-point reduction in mean CAPS score compared to placebo. Discussion: This study is the first to investigate an oral vasopressin 1a receptor antagonist for PTSD. As a wave of PTSD clinical trials with new pharmaceutical compounds are beginning now, lessons learned from our recruitment challenges may be invaluable to these endeavors.
Language	English
Publishing date	2023-03-17
Publishing country	Netherlands
Document type	Journal Article
ISSN	2451-8654
ISSN (online)	2451-8654
DOI	10.1016/j.conctc.2023.101116
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Article: Polyspermy barriers in plants: from preventing to promoting fertilization

Spielman, Melissa / Scott, Rod J

Sexual plant reproduction. 2008 Mar., v. 21, no. 1

2008

Abstract: Barriers to polyspermy (fertilization of a female gamete by more than one sperm) are essential to successful reproduction in a wide range of organisms including mammals, echinoderms, fish, molluscs, and algae. In animals and fucoid algae, polyspermy ... ...

Abstract	Barriers to polyspermy (fertilization of a female gamete by more than one sperm) are essential to successful reproduction in a wide range of organisms including mammals, echinoderms, fish, molluscs, and algae. In animals and fucoid algae, polyspermy results in early death of the zygote due to transmission of extra centrioles from the sperm and consequent disruptions to the mitotic spindle. Accordingly, a variety of mechanisms have evolved to prevent penetration of an egg by more than one sperm, or more than one sperm nucleus from fusing with an egg nucleus. The evolution of internal fertilization has also provided an opportunity to limit the number of sperm that gain access to each egg, as occurs in the mammalian female reproductive tract. Polyspermy and polyspermy barriers in plants have received much less attention. Plants lack centrioles and therefore, polyspermy would not be expected to cause lethal aberrant spindle organization. However, we find evidence from cytological, genetic and in vitro fertilization studies for polyspermy barriers in plants. Angiosperms, like mammals, are internally fertilized, and exert a high level of control over the number of sperm that have access to each female gamete. In particular, regulation of pollen tube growth ensures that in general only two sperm enter each embryo sac, where one fertilizes the egg and the other the central cell. Despite this 1:1 ratio of sperm to gametes within the embryo sac, angiosperms still require a mechanism to ensure that each female gamete is fertilized by one and only one sperm. Here, we present evidence suggesting that a polyspermy block on the egg may be part of the mechanism that promotes faithful double fertilization.
Keywords	Algae ; Echinodermata ; Magnoliophyta ; centrioles ; death ; eggs ; embryo sac ; evolution ; fish ; in vitro fertilization ; mammals ; mitotic spindle apparatus ; molluscs ; pollen ; polyspermy ; spermatozoa ; zygote
Language	English
Dates of publication	2008-03
Size	p. 53-65.
Publisher	Springer-Verlag
Publishing place	Berlin/Heidelberg
Document type	Article
ZDB-ID	1478944-9
ISSN	1432-2145 ; 0934-0882
ISSN (online)	1432-2145
ISSN	0934-0882
DOI	10.1007/s00497-007-0063-7
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Article ; Online: Uptake and acceptability of a mainstreaming model of hereditary cancer multigene panel testing among patients with ovarian, pancreatic, and prostate cancer.

Genetics in medicine : official journal of the American College of Medical Genetics

2021 Volume 23, Issue 11, Page(s) 2105–2113

Abstract: Purpose: To address demands for timely germline information to guide treatments, we evaluated experiences of patients with ovarian, pancreatic, and prostate cancer with a mainstreaming genetic testing model wherein multigene panel testing was ordered by ...

Abstract	Purpose: To address demands for timely germline information to guide treatments, we evaluated experiences of patients with ovarian, pancreatic, and prostate cancer with a mainstreaming genetic testing model wherein multigene panel testing was ordered by oncologists with standardized pretest patient education, and genetic counselors delivered results and post-test genetic counseling via telephone. Methods: Among 1,203 eligible patients, we conducted a prospective single-arm study to examine patient uptake and acceptability (via self-report surveys at baseline and three weeks and three months following result return) of this mainstreaming model. Results: Only 10% of eligible patients declined participation. Among 1,054 tested participants, 10% had pathogenic variants (PV), 16% had variants of uncertain significance (VUS), and 74% had no variant identified (NV). Participants reported high initial acceptability, including high satisfaction with their testing decision. Variability over time in several outcomes existed for participants with PV or NV: those with NV experienced a temporary increase in depression (p Conclusion: This mainstreaming model may offer a feasible approach for extending access to germline genetic information.
MeSH term(s)	Genetic Counseling ; Genetic Predisposition to Disease ; Genetic Testing ; Humans ; Male ; Prospective Studies ; Prostatic Neoplasms/diagnosis ; Prostatic Neoplasms/genetics
Language	English
Publishing date	2021-07-13
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1455352-1
ISSN	1530-0366 ; 1098-3600
ISSN (online)	1530-0366
ISSN	1098-3600
DOI	10.1038/s41436-021-01262-2
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Article: Deeper into the maize: new insights into genomic imprinting in plants.

Scott, Rod J / Spielman, Melissa

BioEssays : news and reviews in molecular, cellular and developmental biology

2006 Volume 28, Issue 12, Page(s) 1167–1171

Abstract: Current models for regulation of parent-specific gene expression in plants have been based on a small number of imprinted genes in Arabidopsis. These present repression as the default state, with expression requiring targeted activation. In general, ... ...

Abstract	Current models for regulation of parent-specific gene expression in plants have been based on a small number of imprinted genes in Arabidopsis. These present repression as the default state, with expression requiring targeted activation. In general, repression is associated with maintenance methylation of cytosines, while no role has been found in Arabidopsis imprinting for de novo methylation--unlike the case in mammals. A recent paper both reinforces and challenges the model drawn from Arabidopsis. Methylation patterns of two imprinted loci in maize were tracked from gametes to offspring, enabling an exploration of the timing of imprinting. For one gene, fie1, the results were as expected: parent-specific methylation patterns were inherited from the three types of gamete: egg, central cell and sperm. The behaviour of fie2, however, was a surprise: no alleles were methylated in the gametes, although paternally contributed fie2 is methylated and silent in the endosperm, indicating that, in some cases, plant imprinting requires de novo DNA methylation. This work significantly broadens our understanding of plant imprinting and points to a greater diversity in imprinting mechanisms than has previously been appreciated.
MeSH term(s)	DNA Methylation ; Fertilization/genetics ; Genes, Plant ; Genomic Imprinting/genetics ; Models, Genetic ; Zea mays/genetics
Language	English
Publishing date	2006-12
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	50140-2
ISSN	1521-1878 ; 0265-9247
ISSN (online)	1521-1878
ISSN	0265-9247
DOI	10.1002/bies.20508
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Zs.A 2024: Show issues

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Article: Epigenetics: imprinting in plants and mammals--the same but different?

Scott, Rod J / Spielman, Melissa

Current biology : CB

2004 Volume 14, Issue 5, Page(s) R201–3

Abstract: Plants and animals both exhibit parental imprinting, but do they control it the same way? Recent studies show that in Arabidopsis, as in mammals, imprinted alleles are subject to DNA methylation--but, surprisingly, the default state is silence rather ... ...

Abstract	Plants and animals both exhibit parental imprinting, but do they control it the same way? Recent studies show that in Arabidopsis, as in mammals, imprinted alleles are subject to DNA methylation--but, surprisingly, the default state is silence rather than activity.
MeSH term(s)	Animals ; DNA Methylation ; Epigenesis, Genetic ; Genomic Imprinting/genetics ; Mammals/genetics ; Mammals/metabolism ; Models, Genetic ; Plants/genetics ; Plants/metabolism ; Suppression, Genetic/genetics
Language	English
Publishing date	2004-03-09
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1071731-6
ISSN	1879-0445 ; 0960-9822
ISSN (online)	1879-0445
ISSN	0960-9822
DOI	10.1016/j.cub.2004.02.022
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Article ; Online: Reference intervals for parameters of health of eastern grey kangaroos Macropus giganteus and management implications across their geographic range

Brandimarti, Maquel E. / Gray, Rachael / Coulson, G. M. / Cripps, Jemma K. / Wilson, Michelle E. / Death, Clare / Snape, Melissa / Wimpenny, Claire / Silva, Fabiola Rodrigues Oliveira / Miller, Emily J. / Scanes, Elliot / Spielman, Derek / Thomas, Georgia / Herbert, Catherine A.

Wildlife biology. 2020 July 8, v. 2020, no. 2, p. wlb.00692

2020

Abstract: Reference intervals (RIs) describe baseline parameters of healthy animals, providing a powerful tool for wildlife managers to monitor health, identify disease and assess animal welfare. This paper reports haematological, glucose and serum protein RIs for ...

Abstract	Reference intervals (RIs) describe baseline parameters of healthy animals, providing a powerful tool for wildlife managers to monitor health, identify disease and assess animal welfare. This paper reports haematological, glucose and serum protein RIs for one of Australia's most iconic and managed mammals, the eastern grey kangaroo Macropus giganteus. Blood samples (n = 514) were collected from 11 populations of eastern grey kangaroos, across much of their geographic range. A species-level RI was initially established based on samples collected from four sites (n = 245) and was further partitioned based on significant differences associated with sexual maturity and season. Unique population means were established from a further seven sites to investigate the importance of biotic (sex and sexual maturity) and abiotic (season, site, rainfall, temperature and laboratory) factors on kangaroo health parameters. Random forest analysis of health parameters revealed that abiotic factors (site, rainfall, temperature and season) were largely responsible for differences in haematological, glucose and serum protein values. Sex was found to have no influence, while sexual maturity and laboratory of analysis had moderate effects. Based on these findings, interpretation of individual and population haematological and serum protein values requires careful consideration of the timing of sample collection, environmental conditions and sexual maturity. When assessing kangaroo health, the relevant sexual maturity RI must be considered initially. For populations with similarities to those described (for example high density or captive populations) users should also consider site specific mean haematological and serum protein values. The RIs reported are valuable when establishing the health status of kangaroo populations. Furthermore, understanding the influence of biotic and abiotic factors will improve the utility of these RIs to assess health, disease status and improve welfare in eastern grey kangaroos.
Keywords	Macropus giganteus ; animal welfare ; blood ; blood proteins ; geographical distribution ; glucose ; health status ; rain ; sexual maturity ; temperature ; wildlife ; Australia
Language	English
Dates of publication	2020-0708
Size	p. wlb.00692
Publishing place	Nordic Board for Wildlife Research
Document type	Article ; Online
Note	NAL-AP-2-clean
ZDB-ID	1291831-3
ISSN	0909-6396
ISSN	0909-6396
DOI	10.2981/wlb.00692
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