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Article ; Online: Desmoplakin (Dsp) conditional knockout in NR5A1+ somatic cells affects germ cell survival in developing mouse gonads.

Piprek, Rafal P / Rams-Pociecha, Izabela / Zdanowski, Robert / Kloc, Malgorzata / Kubiak, Jacek Z

2022 Volume 163, Issue 4, Page(s) 199–207

Abstract: Cell to cell interactions are crucial for morphogenesis and tissue formation. Desmoplakin (encoded by the Dsp gene) is a component of desmosomes and anchors the transmembrane adhesion proteins to the cytoskeleton. Its role in gonad development remains ... ...

Abstract	Cell to cell interactions are crucial for morphogenesis and tissue formation. Desmoplakin (encoded by the Dsp gene) is a component of desmosomes and anchors the transmembrane adhesion proteins to the cytoskeleton. Its role in gonad development remains vague. To study the role of desmoplakin in gonad development, we used a tissue-specific knockout of the Dsp gene in the NR5A1+ somatic cells of the gonads. We show here that desmoplakin is necessary for the survival of germ cells in fetal testes and ovaries. The Dspknockout in NR5A1+ somatic cells in testes decreased the number of germ cells, and thus the size of the testes, but did not affect the Sertoli cells or the structure of testis cords and interstitium. The Dspknockout in NR5A1+ somatic cells in ovaries decreased the number of female germ cells and drastically reduced the formation of ovarian follicles. Dsp knockout in NR5A1+ somatic cells did not affect the sex determination and sexual differentiation of the gonads, as judged from an unchanged expression of genes essential for these processes. We conclude that mediation by desmoplakin cell adhesion between the gonadal cells is necessary for germ cell survival.
MeSH term(s)	Animals ; Cell Survival ; Desmoplakins/genetics ; Desmoplakins/metabolism ; Female ; Germ Cells ; Gonads ; Male ; Mice ; Sex Differentiation ; Testis/metabolism
Chemical Substances	Desmoplakins
Language	English
Publishing date	2022-02-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2034501-X
ISSN	1741-7899 ; 1470-1626 ; 1476-3990
ISSN (online)	1741-7899
ISSN	1470-1626 ; 1476-3990
DOI	10.1530/REP-21-0295
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Book: Handlungsanleitung zur Lärmminderung in blechverarbeitenden Betrieben der Hausgeräteindustrie

Piprek, Philipp / Rüter, R.

(Schriftenreihe der Bundesanstalt für Arbeitsschutz : Forschung ; 625 ; Forschungsberichtsreihe Arbeit und Technik)

1990

Author's details	von Ph. Piprek und R. Rüter
Series title	Schriftenreihe der Bundesanstalt für Arbeitsschutz : Forschung ; 625 Forschungsberichtsreihe Arbeit und Technik Schriftenreihe der Bundesanstalt für Arbeitsschutz Schriftenreihe der Bundesanstalt für Arbeitsschutz ; Forschung
Collection	Schriftenreihe der Bundesanstalt für Arbeitsschutz Schriftenreihe der Bundesanstalt für Arbeitsschutz ; Forschung
Keywords	Noise, Occupational / prevention & control / outlines ; Metallurgy ; Household Articles ; Die Kunst ; École de Paris ; Geschichte 1983-1988
Subject	Paris
Language	German
Size	125 S. : Ill., graph. Darst.
Publisher	Wirtschaftsverl. NW, Verl. für Neue Wiss
Publishing place	Bremerhaven
Document type	Book
HBZ-ID	HT003770198
ISBN	3-89429-049-8 ; 3-88314-846-6 ; 978-3-89429-049-8 ; 978-3-88314-846-5
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Article: Genetic mechanisms underlying male sex determination in mammals.

Piprek, R P

Journal of applied genetics

2009 Volume 50, Issue 4, Page(s) 347–360

Abstract: Genetic control of gonadal development proceeds through either the male or female molecular pathways, driving bipotential gonadal anlage differentiation into a testis or ovary. Antagonistic interactions between the 2 pathways determine the gonadal sex. ... ...

Abstract	Genetic control of gonadal development proceeds through either the male or female molecular pathways, driving bipotential gonadal anlage differentiation into a testis or ovary. Antagonistic interactions between the 2 pathways determine the gonadal sex. Essentially sex determination is the enhancement of one of the 2 pathways according to genetic sex. Initially, Sry with other factors upregulates Sox9 expression in XY individuals. Afterwards the expression of Sox9 is maintained by a positive feedback loop with Fgf9 and prostaglandin D2 as well as by autoregulative ability of Sox9. If these factors reach high concentrations, then Sox9 and/or Fgf9 may inhibit the female pathway. Surprisingly, splicing, nuclear transport, and extramatrix proteins may be involved in sex determination. The male sex determination pathway switches on the expression of genes driving Sertoli cell differentiation. Sertoli cells orchestrate testicular differentiation. In the absence of Sry, the predomination of the female pathway results in the realization of a robust genetic program that drives ovarian differentiation.
MeSH term(s)	Animals ; Disorders of Sex Development ; Female ; Fibroblast Growth Factor 9/genetics ; Fibroblast Growth Factor 9/physiology ; Genes, sry ; Male ; Mammals/genetics ; Mammals/physiology ; Models, Genetic ; Ovary/embryology ; Paracrine Communication ; Prostaglandin D2/physiology ; SOX9 Transcription Factor/genetics ; Sex Determination Processes ; Sex Differentiation/genetics ; Sex Differentiation/physiology ; Testis/embryology ; Transcription Factors/genetics
Chemical Substances	DMRT1 protein ; Fibroblast Growth Factor 9 ; SOX9 Transcription Factor ; Transcription Factors ; Prostaglandin D2 (RXY07S6CZ2)
Language	English
Publishing date	2009-10-29
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1235302-4
ISSN	2190-3883 ; 1234-1983
ISSN (online)	2190-3883
ISSN	1234-1983
DOI	10.1007/BF03195693
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Article: Molecular mechanisms underlying female sex determination--antagonism between female and male pathway.

Piprek, Rafał P

Folia biologica

2009 Volume 57, Issue 3-4, Page(s) 105–113

Abstract: ... participating in the establishment of the ovary fate. The loss of functional R-spondin1 causes the complete ... to R-spondin1 in the gonad but also is decisive for the ovarian fate. WNT4 and RSPO1 drive ...

Abstract	Molecular interactions in a developing gonad are crucial for an individual since they determine its phenotypic sex. The process of sex determination is complicated because of the antagonistic interactions between the male and female pathway. Factors responsible for the determination of femaleness make the female pathway. This pathway has to inhibit a complex network of male-determining factors and also has to induce the expression of genes that drive differentiation of the ovary. Morphological description of the ovary development suggests that this process is simple, however, the analysis of the robust gene expression indicates that genetic control of the ovary differentiation is active and complicated at the molecular level. A plethora of genes is expresed in developing gonads. Nevertheless, there are only a couple of genes the role in ovary development of which has been described till now. RSPO1 seems the main gene participating in the establishment of the ovary fate. The loss of functional R-spondin1 causes the complete female-to-male sex reversal in human. The second important factor is WNT4 which plays an opposite role to R-spondin1 in the gonad but also is decisive for the ovarian fate. WNT4 and RSPO1 drive the disposition of beta-catenin in cells and thus these factors regulate gene transcription and cell-cell adhesion. Foxl2 is another gene contributing to the development of the ovary. In females also germ cells seem to play important role in sex determination.
MeSH term(s)	Animals ; Cell Adhesion/genetics ; Cell Adhesion/physiology ; Female ; Forkhead Transcription Factors/genetics ; Forkhead Transcription Factors/physiology ; Humans ; Male ; Ovary/physiology ; Sex Determination Processes ; Testis/physiology ; Thrombospondins/genetics ; Thrombospondins/physiology ; Transcription, Genetic/genetics ; Transcription, Genetic/physiology ; Wnt Proteins/genetics ; Wnt Proteins/physiology ; Wnt4 Protein ; beta Catenin/genetics ; beta Catenin/physiology
Chemical Substances	CTNNB1 protein, human ; Forkhead Transcription Factors ; RSPO1 protein, human ; Thrombospondins ; WNT4 protein, human ; Wnt Proteins ; Wnt4 Protein ; beta Catenin
Language	English
Publishing date	2009-09-15
Publishing country	Poland
Document type	Journal Article ; Review
ZDB-ID	130070-2
ISSN	1734-9168 ; 0015-5497
ISSN (online)	1734-9168
ISSN	0015-5497
DOI	10.3409/fb57_3-4.105-113
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Article ; Online: Demand-side financing in the form of baby packages in Northern Mozambique

Anita Makins / Jochen Ehmer / Alexandra Piprek / Francisco Mbofana / Amanda Ross / Michael André Hobbins

PLoS ONE, Vol 14, Iss 5, p e

Results from an observational study.

2019 Volume 0215282

Abstract: ... rate ratio of institutional births in the intervention district was 1.80 (95% CI 1.72, 1.89 p<0.001 ...

Abstract	Background The Maternal Mortality Ratio in Mozambique has stagnated at 405 deaths per 100,000 live births with virtually no progress over the last 15 years. Low Institutional Birth Rates (IBRs) levelling around 50% in many rural areas constitute one of the contributing reasons. Demand-side financing has successfully increased usage of maternal health services in other countries, but little information exists on in-kind incentives in rural Africa. The objective was to test the impact on Institutional Birth Rates of giving a USD 5.50 baby package incentive to every woman who came to give birth in a health centre in a rural, poor district of Cabo Delgado, Mozambique. Methods and findings The intervention was implemented in one district in 2010 with the remaining 15 districts serving as controls. The total population in the 16 districts in 2006 was just under 1.5 million people. IBRs were observed from 2006 to 2013 (53 months before and 55 months after the intervention began). The non-intervention districts showed a slight increase, from a mean IBR of 0.39 (SD = 0.10) in 2006 to 0.67 (SD = 0.13) in 2014. The intervention district had a dramatic increase in IBRs within six months of the start of the intervention in 2010, which was sustained until the end of the study. Adjusting for the background increase and for confounders, including health facilities and health personnel per district, and taking clustering in districts into account, the estimated rate ratio of institutional births in the intervention district was 1.80 (95% CI 1.72, 1.89 p<0.001). Conclusion Women were almost twice as likely to have an institutional birth following the introduction of the baby package.
Keywords	Medicine ; R ; Science ; Q
Subject code	360
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
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Article ; Online: Sexual dimorphism of AMH, DMRT1 and RSPO1 localization in the developing gonads of six anuran species.

Piprek, Rafal P / Pecio, Anna / Laskowska-Kaszub, Katarzyna / Kubiak, Jacek Z / Szymura, Jacek M

The International journal of developmental biology

2013 Volume 57, Issue 11-12, Page(s) 891–895

Abstract: ... Sertoli cells. Interestingly, in B. viridis, R. arvalis and R. temporaria, AMH was also expressed in ovaries ...

Abstract	In vertebrates, several genes which are differentially expressed in various species, have been implicated in sex determination and gonadal differentiation. We used immunolocalization to study the expression pattern of three proteins AMH, DMRT1, RSPO1 involved in the sexual differentiation of gonads. The pattern of AMH, DMRT1 and RSPO1 expression was analyzed in X. laevis and in five other divergent anuran species: Bombina bombina, Bufo viridis, Hyla arborea, Rana arvalis and Rana temporaria during gonadal development. The pattern of expression of AMH in the developing testes of six studied anuran species was similar to that described for other vertebrates. AMH was strongly expressed in differentiating Sertoli cells. Interestingly, in B. viridis, R. arvalis and R. temporaria, AMH was also expressed in ovaries. In all studied species, DMRT1 was highly expressed in the developing testes, in both the somatic and germ cells. It was also expressed at low level in ovaries in all studied species, with the exception of H. arborea. RSPO1 was expressed in the developing ovaries, especially in the somatic cells, and was almost undetectable in developing testes in all examined anurans. These developmental expression patterns strongly suggest an involvement of AMH and DMRT1 in the development of male gonads and of RSPO1 in the female gonads. The differences in the expression patterns of these proteins in the gonads of different species might reflect the diversity of gonadal development patterns in anurans resulting from long lasting and diverged paths of their evolution.
MeSH term(s)	Animals ; Anti-Mullerian Hormone/metabolism ; Anura/growth & development ; Bufonidae ; Female ; Germ Cells/cytology ; Male ; Ranidae ; Sex Characteristics ; Species Specificity ; Thrombospondins/metabolism ; Transcription Factors/metabolism ; Xenopus laevis
Chemical Substances	DMRT1 protein ; Thrombospondins ; Transcription Factors ; Anti-Mullerian Hormone (80497-65-0)
Language	English
Publishing date	2013
Publishing country	Spain
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1036070-0
ISSN	1696-3547 ; 0214-6282
ISSN (online)	1696-3547
ISSN	0214-6282
DOI	10.1387/ijdb.130192rp
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Article ; Online: Retinoic acid homeostasis regulates meiotic entry in developing anuran gonads and in Bidder's organ through Raldh2 and Cyp26b1 proteins.

Piprek, Rafal P / Pecio, Anna / Laskowska-Kaszub, Katarzyna / Kloc, Malgorzata / Kubiak, Jacek Z / Szymura, Jacek M

Mechanisms of development

2013 Volume 130, Issue 11-12, Page(s) 613–627

Abstract: ... distinct expression patterns specific for (i) X. laevis, H. arborea, R. arvalis, R. temporaria and (ii) B ...

Abstract	The vitamin A (retinol) and its metabolites such as retinoic acid (RA) affect vertebrate gametogenesis. The level of RA in cells relies on the balance between its synthesis and degradation. The sex-dependent equilibrium is reached in different ways in various species. It is known that RA induces meiosis in developing gonads in mouse, chicken and urodel amphibians, but its role in anuran amphibians has not been studied. Here we show in six anuran species (Xenopus laevis, Bombina bombina, Hyla arborea, Bufo viridis, Rana arvalis and Rana temporaria) that cultured undifferentiated gonads were insensitive to RA treatment, but the RA induced ectopic meiosis in cultured larval testes. In larval testes of all studied species, the exogenous RA induced leptotene phase of I meiotic prophase in gonia, but only in H. arborea and B. viridis gonia progressed to zygotene phase. In the cultured developing ovaries, exogenous RA led to increase in the number of oocytes as compared to the control. Inhibition of either RA synthesis or RA-receptors prevented meiotic entry in larval gonads of all species. Exogenous RA rescued this inhibitory effect demonstrating that the balance in RA homeostasis plays a key role in meiotic entry in anuran gonads. The localization of two enzymes, Raldh2 and Cyp26b1, which antagonistically control RA levels and whose abundance suggests the sites of RA synthesis and degradation respectively, showed two distinct expression patterns specific for (i) X. laevis, H. arborea, R. arvalis, R. temporaria and (ii) B. bombina, B. viridis. Thus, RA, in correlation with specific expression patterns of Raldh2 and Cyp26b, induces meiosis during gonad development in anurans. In addition, in B. viridis, RA signalling seems important for development of the Bidder's organ containing oocytes both in males and females.
MeSH term(s)	Aldehyde Oxidase/genetics ; Aldehyde Oxidase/metabolism ; Animals ; Anura/genetics ; Anura/growth & development ; Anura/metabolism ; Cytochrome P-450 Enzyme System/genetics ; Cytochrome P-450 Enzyme System/metabolism ; Female ; Gene Expression Regulation, Developmental ; Homeostasis ; Larva/genetics ; Larva/growth & development ; Larva/metabolism ; Male ; Meiosis ; Oocytes/growth & development ; Oocytes/metabolism ; Ovary/growth & development ; Ovary/metabolism ; Retinoic Acid 4-Hydroxylase ; Sex Differentiation ; Signal Transduction ; Species Specificity ; Spermatogonia/growth & development ; Spermatogonia/metabolism ; Testis/growth & development ; Testis/metabolism ; Tretinoin/metabolism ; Xenopus Proteins/genetics ; Xenopus Proteins/metabolism
Chemical Substances	Xenopus Proteins ; Tretinoin (5688UTC01R) ; Cytochrome P-450 Enzyme System (9035-51-2) ; Retinoic Acid 4-Hydroxylase (EC 1.14.14.1) ; aldh1a2 protein, Xenopus (EC 1.2.1.36) ; Aldehyde Oxidase (EC 1.2.3.1)
Language	English
Publishing date	2013-11
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1055986-3
ISSN	1872-6356 ; 0925-4773
ISSN (online)	1872-6356
ISSN	0925-4773
DOI	10.1016/j.mod.2013.09.001
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Article: Some potentially important indigenous aromatic plants for the Eastern Seaboard areas of Southern Africa

Piprek, S.R.K / Graven, E.H / Whitfield, P

World crops: production, utilization, description. 1982. v. 10 (4)

1982

Title variant	Some potentially important indigenous aromatic plants for the Eastern Seaboard areas of Southern Africa [Artemisia afra, Pteronia incana, Eriocephalus punctulatus].
Keywords	South Africa
Language	English
Size	p. 255-263., ill.
Document type	Article
Database	NAL-Catalogue (AGRICOLA)

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Article: Some potentially important indigenous aromatic plants for the Eastern Seaboard areas of Southern Africa

Piprek, S.R.K / Graven, E.H / Whitfield, P

World crops: production, utilization, description. 1982. v. 7

1982

Title variant	Some potentially important indigenous aromatic plants for the Eastern Seaboard areas of Southern Africa [Production of essential oils].
Keywords	South Africa
Language	English
Dates of publication	1982-1978
Size	p. 255-263.
Document type	Article
Database	NAL-Catalogue (AGRICOLA)

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