Article ; Online: Generation of an induced pluripotent stem cell (iPSC) line (IMAGINi007) from a patient with steroid-resistant nephrotic syndrome carrying the homozygous p.R138Q mutation in the podocin-encoding NPHS2 gene.
2020 Volume 46, Page(s) 101878
Abstract: Mutations in the NPHS2 gene, encoding podocin, are responsible for the majority of familial cases of steroid-resistant nephrotic syndrome (SRNS), a rare glomerulopathy that rapidly progresses to end-stage renal disease. We obtained peripheral blood ... ...
Abstract | Mutations in the NPHS2 gene, encoding podocin, are responsible for the majority of familial cases of steroid-resistant nephrotic syndrome (SRNS), a rare glomerulopathy that rapidly progresses to end-stage renal disease. We obtained peripheral blood mononuclear cells (PBMCs) from a patient carrying the homozygous c.413G>A substitution (p.R138Q) in NPHS2 gene, which is the most prevalent mutation in the European population. The PBMCs were reprogrammed by non-integrative viral transduction of the Yamanaka's factors. The resulting iPSCs display normal karyotype, express pluripotency hallmarks and are capable of multilineage differentiation, offering a useful tool to study pathological mechanisms of SRNS and perform drug testing. |
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MeSH term(s) | Humans ; Induced Pluripotent Stem Cells ; Intracellular Signaling Peptides and Proteins ; Leukocytes, Mononuclear ; Membrane Proteins ; Mutation ; Nephrotic Syndrome/genetics ; Steroids/therapeutic use |
Chemical Substances | Intracellular Signaling Peptides and Proteins ; Membrane Proteins ; NPHS2 protein ; Steroids |
Language | English |
Publishing date | 2020-06-18 |
Publishing country | England |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2393143-7 |
ISSN | 1876-7753 ; 1873-5061 |
ISSN (online) | 1876-7753 |
ISSN | 1873-5061 |
DOI | 10.1016/j.scr.2020.101878 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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