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Article: Extremophile enzyme activity lab: using catalase from

Scott, Joseph W / Steel, J Jordan

Journal of microbiology & biology education

2024 Volume 25, Issue 1, Page(s) e0006523

Abstract: There are places on earth that are considered to possess extreme physico-chemical characteristics as they relate to life. Surprisingly, there are microbes that have adapted various strategies that enable them to form robust communities in these ... ...

Abstract	There are places on earth that are considered to possess extreme physico-chemical characteristics as they relate to life. Surprisingly, there are microbes that have adapted various strategies that enable them to form robust communities in these environments. The microbes that live in these environments, called extremophiles, are described as being thermophilic, psychrophilic, halophilic, acidophilic, alkaliphilic, barophilic, and so on. Given that extremophiles were not discovered until relatively recently due to a view point that the environments in which they inhabited were not conducive to life, it is reasonable to conclude that the concept of extremophiles may be hard to grasp for students. Herein is described a laboratory exercise adapted from laboratory exercises that use mesophilic catalase enzymes to illustrate the influence of physico-chemical parameters on enzyme activity. Catalase is an enzyme that accelerates the degradation of hydrogen peroxide to water and oxygen gas. In addition to mesophilic catalases, the catalase from
Language	English
Publishing date	2024-01-08
Publishing country	United States
Document type	Journal Article
ISSN	1935-7877
ISSN	1935-7877
DOI	10.1128/jmbe.00065-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Genome Analysis of SARS-CoV-2 Case Study: An Undergraduate Online Learning Activity To Introduce Bioinformatics, BLAST, and the Power of Genome Databases.

Steel, J Jordan

Journal of microbiology & biology education

2021 Volume 22, Issue 1

Language	English
Publishing date	2021-03-15
Publishing country	United States
Document type	Journal Article
ISSN	1935-7877
ISSN	1935-7877
DOI	10.1128/jmbe.v22i1.2245
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Article ; Online: Biotechnology Immersion Program: professional development where the participants do the preparation, teaching, and outreach to maximize learning gains.

Bates, Katherine L / Hung, Chia / Steel, J Jordan

FEMS microbiology letters

2022 Volume 369, Issue 1

Abstract: Professional development for teachers of primary, intermediate, and secondary schools (Kindergarten to Grade 12; K-12), especially for highly technical subjects such as Microbial Biotechnology, can involve arduous and ineffective training methods ... ...

Abstract	Professional development for teachers of primary, intermediate, and secondary schools (Kindergarten to Grade 12; K-12), especially for highly technical subjects such as Microbial Biotechnology, can involve arduous and ineffective training methods prioritizing content delivery over sound pedagogical techniques. Teachers are learning complex content, techniques, and pedagogies but have little time to practice or gain experience and confidence in their newly acquired skills. The Biotechnology Immersion Program (BiP) sought to overcome this challenge by incorporating an intentional immersive experiential system into professional development; teachers learn new content, experience hands-on activities, and work through assessments in the role of a student while experienced subject matter expert faculty run the teaching and activities. Afterwards, the teachers get the opportunity to switch roles and practice teaching, running, and managing the same learning activities that they just experienced. The faculty experts are available to mentor, guide, and direct the teachers as they try out teaching and implementing novel biotechnology classroom activities. BiP focused on three critical aspects of successful professional development: time, personal experience, and connection. This mentored teaching and implementation practice system provided a robust professional development platform, where educators felt prepared and confident to run new biotechnology lab activities in their own classrooms.
MeSH term(s)	Humans ; Immersion ; Learning ; Schools ; Faculty ; Biotechnology
Language	English
Publishing date	2022-11-17
Publishing country	England
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	752343-9
ISSN	1574-6968 ; 0378-1097
ISSN (online)	1574-6968
ISSN	0378-1097
DOI	10.1093/femsle/fnac111
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Article: Microbial Murders Crime Scene Investigation: An Active Team-Based Learning Project that Enhances Student Enthusiasm and Comprehension of Clinical Microbial Pathogens.

Steel, J Jordan

Journal of microbiology & biology education

2017 Volume 18, Issue 2

Abstract: Microbial disease knowledge is a critical component of microbiology courses and is beneficial for many students' future careers. Microbiology courses traditionally cover core concepts through lectures and labs, but specific instruction on microbial ... ...

Abstract	Microbial disease knowledge is a critical component of microbiology courses and is beneficial for many students' future careers. Microbiology courses traditionally cover core concepts through lectures and labs, but specific instruction on microbial diseases varies greatly depending on the instructor and course. A common project involves students researching and presenting a disease to the class. This method alone is not very effective, and course evaluations have consistently indicated that students felt they lacked adequate disease knowledge; therefore, a more hands-on and interactive disease project was developed called Microbial Murders. For this team-based project, a group of students chooses a pathogen, researches the disease, creates a "mugshot" of the pathogen, and develops a corresponding "crime scene," where a hypothetical patient has died from the microbe. Each group gives a presentation introducing the microbial pathogen, signs/symptoms, treatments, and overall characteristics. The students then visit each other's crime scenes to match the pathogen with the correct crime scene by critically thinking through the clues. This project has shown remarkable success. Surveys indicate that 73% of students thought the project helped them understand the material and 84% said it was worth their time. Student participation, excitement, understanding, and application of microbial disease knowledge have increased and are evident through an increase in course evaluations and in student assessment scores. This project is easy to implement and can be used in a wide variety of biology, microbiology, or health classes for any level (middle school through college).
Language	English
Publishing date	2017-08-11
Publishing country	United States
Document type	Journal Article
ISSN	1935-7877
ISSN	1935-7877
DOI	10.1128/jmbe.v18i2.1298
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Article ; Online: Designing a Wastewater-Based Epidemiology Study at the U.S. Air Force Academy: Using Severe Acute Respiratory Syndrome Coronavirus 2 to Test a Sentinel System for Early Disease Outbreak Detection.

Goodwin, Jordan M / Golder, Philip M / LeClair, Amy R / Jun, Samuel C / Huckstep, Odaro J / Steel, J Jordan / Balboni, Armand L

Military medicine

2023 Volume 188, Issue 11-12, Page(s) e3675–e3679

Abstract: Introduction: The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) in wastewater has been proposed as a sentinel surveillance epidemiological tool for detection of infectious disease at a community level and as a complementary ... ...

Abstract	Introduction: The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) in wastewater has been proposed as a sentinel surveillance epidemiological tool for detection of infectious disease at a community level and as a complementary approach to syndromic surveillance of infectious disease outbreaks. We have designed a study to test the presence and quantity of SARS-CoV2, the virus responsible for COVID19, in the wastewater treatment facility (WWTF) of the U.S. Air Force Academy. Materials and methods: Wastewater samples were tested in the laboratory to quantify the amount of SARS-CoV2 RNA using reverse transcription-quantitative polymerase chain reaction. Raw SARS-CoV2 viral titer in wastewater was normalized to the viral titer of a fecal marker, pepper mild mottle virus, to correct for dilutions. Temporal and spatial trends of COVID19 were analyzed. Furthermore, we compared wastewater analysis results against clinical data to assist public health decisions. Results: Preliminary data suggest that wastewater analysis can provide temporal and spatial trends of COVID19. The geographically discrete WWTF at the U.S. Air Force suggests that wastewater testing is a useful approach to developing a comprehensive sentinel surveillance system. Conclusions: Together with ongoing syndromic surveillance data, this proof-of-concept study seeks to determine whether early detection of SARS-CoV2 in a closed system WWTF correlates to changes in community and clinically reported COVID19. The well-documented population served by the geographically discrete WWTF at the U.S. Air Force Academy may serve to better elucidate the adjunctive role of wastewater testing in a comprehensive surveillance system. These results may be of particular interest to the DoD and local commanders given the WWTFs under their immediate control and the information that these studies may provide in support of operational readiness through early detection of disease outbreaks.
MeSH term(s)	Humans ; SARS-CoV-2 ; COVID-19/diagnosis ; COVID-19/epidemiology ; RNA, Viral ; Wastewater ; Wastewater-Based Epidemiological Monitoring ; Disease Outbreaks ; Early Diagnosis
Chemical Substances	RNA, Viral ; Wastewater
Language	English
Publishing date	2023-07-04
Publishing country	England
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	391061-1
ISSN	1930-613X ; 0026-4075
ISSN (online)	1930-613X
ISSN	0026-4075
DOI	10.1093/milmed/usad239
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Article ; Online: Involvement of the endocannabinoid system in the inhibition of Sindbis virus replication: a preliminary study.

Rodriguez, Juan L / Lopez, Joseph A / Steel, J Jordan

Journal of cannabis research

2021 Volume 3, Issue 1, Page(s) 10

Abstract: Background: Sindbis virus (Alphaviridae) is a plus-strand RNA virus that is dependent on the host cell for replication. Cannabinoid (CB) receptors are found on most human cells, including virally infected cells. Activation of cannabinoid receptors has ... ...

Abstract	Background: Sindbis virus (Alphaviridae) is a plus-strand RNA virus that is dependent on the host cell for replication. Cannabinoid (CB) receptors are found on most human cells, including virally infected cells. Activation of cannabinoid receptors has been shown to alter normal cellular physiology. This study aimed to assess how agonist (ACEA) or antagonists/inverse agonist (AM251) of the cannabinoid receptors would alter the cellular environment and impact Sindbis virus replication. Methods: Human hepatoma (Huh7) cells were used as our model for viral replication. Cells were infected with Sindbis virus (SINV) and then treated with CB agonist (ACEA) (10 μM) or antagonist/inverse agonist (AM-251) (10 μM) and virus replication was monitored. A double subgenomic Sindbis virus containing a green fluorescent protein (GFP) reporter gene inserted into a 3' subgenomic promoter was utilized for these assays to quickly measure viral replication. GFP fluorescent cells were analyzed using flow cytometry to measure the percentage of cells expressing the viral reporter and also quantify the levels of GFP fluorescence. Result: Treatment of SINV-infected Huh7 cells with CB1 receptor antagonist/inverse agonist (AM251, 10 μM) resulted in a significant decrease in viral replication, while infected cells treated with a CB1 receptor agonist (ACEA, 10 μM) resulted in a significant increase of viral infection. The data indicates that activation of CB1 receptor by cannabinoids significantly influences the ability of Sindbis virus to replicate in the host cell. Conclusion: Blocking CB1 receptor activity with 10 μM AM251 reduced viral replication, but activating the CB1 receptor with 10 μM ACEA resulted in an increase in viral infection. These results indicate cannabinoids may significantly impact a virus replicating in human liver cells. Future confirmation with other viruses and cell lines will be performed to better understand the impact of cannabinoids on viral infections.
Language	English
Publishing date	2021-04-23
Publishing country	England
Document type	Journal Article
ISSN	2522-5782
ISSN (online)	2522-5782
DOI	10.1186/s42238-021-00068-y
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Article ; Online: Microbial Murders Crime Scene Investigation

J. Jordan Steel

Journal of Microbiology & Biology Education, Vol 18, Iss

An Active Team-Based Learning Project that Enhances Student Enthusiasm and Comprehension of Clinical Microbial Pathogens

2017 Volume 2

Abstract: Microbial disease knowledge is a critical component of microbiology courses and is beneficial for many students’ future careers. Microbiology courses traditionally cover core concepts through lectures and labs, but specific instruction on microbial ... ...

Abstract	Microbial disease knowledge is a critical component of microbiology courses and is beneficial for many students’ future careers. Microbiology courses traditionally cover core concepts through lectures and labs, but specific instruction on microbial diseases varies greatly depending on the instructor and course. A common project involves students researching and presenting a disease to the class. This method alone is not very effective, and course evaluations have consistently indicated that students felt they lacked adequate disease knowledge; therefore, a more hands-on and interactive disease project was developed called Microbial Murders. For this team-based project, a group of students chooses a pathogen, researches the disease, creates a “mugshot” of the pathogen, and develops a corresponding “crime scene,” where a hypothetical patient has died from the microbe. Each group gives a presentation introducing the microbial pathogen, signs/symptoms, treatments, and overall characteristics. The students then visit each other’s crime scenes to match the pathogen with the correct crime scene by critically thinking through the clues. This project has shown remarkable success. Surveys indicate that 73% of students thought the project helped them understand the material and 84% said it was worth their time. Student participation, excitement, understanding, and application of microbial disease knowledge have increased and are evident through an increase in course evaluations and in student assessment scores. This project is easy to implement and can be used in a wide variety of biology, microbiology, or health classes for any level (middle school through college).
Keywords	Active learning ; Team-based ; Crime Scene Investigation ; Infectious disease project ; classroom or lab exercise ; Bloom’s taxonomy ; Special aspects of education ; LC8-6691 ; Biology (General) ; QH301-705.5
Subject code	360
Language	English
Publishing date	2017-09-01T00:00:00Z
Publisher	American Society for Microbiology
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Microbial Murders Crime Scene Investigation

J. Jordan Steel

Journal of Microbiology & Biology Education, Vol 18, Iss

An Active Team-Based Learning Project that Enhances Student Enthusiasm and Comprehension of Clinical Microbial Pathogens

2017 Volume 2

Abstract	Microbial disease knowledge is a critical component of microbiology courses and is beneficial for many students’ future careers. Microbiology courses traditionally cover core concepts through lectures and labs, but specific instruction on microbial diseases varies greatly depending on the instructor and course. A common project involves students researching and presenting a disease to the class. This method alone is not very effective, and course evaluations have consistently indicated that students felt they lacked adequate disease knowledge; therefore, a more hands-on and interactive disease project was developed called Microbial Murders. For this team-based project, a group of students chooses a pathogen, researches the disease, creates a “mugshot” of the pathogen, and develops a corresponding “crime scene,” where a hypothetical patient has died from the microbe. Each group gives a presentation introducing the microbial pathogen, signs/symptoms, treatments, and overall characteristics. The students then visit each other’s crime scenes to match the pathogen with the correct crime scene by critically thinking through the clues. This project has shown remarkable success. Surveys indicate that 73% of students thought the project helped them understand the material and 84% said it was worth their time. Student participation, excitement, understanding, and application of microbial disease knowledge have increased and are evident through an increase in course evaluations and in student assessment scores. This project is easy to implement and can be used in a wide variety of biology, microbiology, or health classes for any level (middle school through college).
Keywords	Special aspects of education ; LC8-6691 ; Biology (General) ; QH301-705.5
Subject code	360
Language	English
Publishing date	2017-09-01T00:00:00Z
Publisher	American Society for Microbiology
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Investing in our nation's future military leaders' synthetic biology knowledge to understand and recognize threats and applications.

Steel, J Jordan / Bates, Katherine L / Barnhart, Michael D

Synthetic biology (Oxford, England)

2019 Volume 4, Issue 1, Page(s) ysz024

Abstract: Synthetic biology encompasses some of the greatest advancements in biology. With improvements in molecular methods and techniques that allow targeted and highly efficient genome manipulation, the capabilities of engineering biology have significantly ... ...

Abstract	Synthetic biology encompasses some of the greatest advancements in biology. With improvements in molecular methods and techniques that allow targeted and highly efficient genome manipulation, the capabilities of engineering biology have significantly increased. These enhancements in biotechnology represent significant potential benefits and risks to the global population. It is important that future leaders are trained and understand the incredible benefits, opportunities and risks associated with synthetic biology. The US Department of Defense (DoD) has issued a technical assessment on the future opportunities of synthetic biology and has encouraged the military institutions to expand and encourage bioengineering research programs. At the US Air Force Academy (USAFA), opportunities are provided for future Air Force officers to recognize the potential and risks associated with synthetic biology by participating in the USAFA Synthetic Biology Education Program (USBEP). Cadets can enroll in synthetic biology courses to learn and master molecular biology techniques and work on independent undergraduate research projects. In addition, cadets have the opportunity to join the USAFA's International Genetically Engineered Machine (iGEM) team and compete in the international synthetic biology competition. This report includes details on how USAFA has recruited, enrolled and encouraged synthetic biology research and education among future leaders in the US Air Force.
Language	English
Publishing date	2019-10-07
Publishing country	England
Document type	Journal Article
ISSN	2397-7000
ISSN (online)	2397-7000
DOI	10.1093/synbio/ysz024
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Article ; Online: Sindbis Virus Replication Reduces Dependence on Mitochondrial Metabolism During Infection.

Rodriguez, Juan L / Costlow, Jessica L / Sheedy, Max / Yoon, Kelly T / Gabaldón, Annette M / Steel, J Jordan

Frontiers in cellular and infection microbiology

2022 Volume 12, Page(s) 859814

Abstract: Alphaviruses are single stranded, positive sense RNA viruses that are often transmitted through mosquito vectors. With the increasing spread of mosquito populations throughout the world, these arboviruses represent a significant global health concern. ... ...

Abstract	Alphaviruses are single stranded, positive sense RNA viruses that are often transmitted through mosquito vectors. With the increasing spread of mosquito populations throughout the world, these arboviruses represent a significant global health concern. Viruses such as Sindbis Virus (SINV), Chikungunya Virus (CHIKV) and Equine Encephalitis Viruses (EEV) are all alphaviruses. As viruses, these pathogens are dependent on the host cell environment for successful viral replication. It has been observed that viruses manipulate cellular metabolism and mitochondrial shape, activity, and dynamics to favor viral infection. This report looked to understand the metabolic changes present during Sindbis virus infection of hamster and human kidney cells. Cells were infected with increasing levels of SINV and at 24 hours post infection the mitochondria morphology was assessed with staining and mitochondrial activity was measured with a real-time Seahorse Bioanalyzer. The relative amount of mitochondrial staining intensity decreased with Sindbis virus infected cells. Both oxygen consumption rate and ATP production were decreased during SINV infection while non-mitochondrial respiration and extracellular acidification rate increased during infection. Collectively, the data indicates that SINV primarily utilizes non-mitochondrial metabolism to support viral infection within the first 24 hours. This understanding of viral preference for host cell metabolism may provide critical targets for antiviral therapies and help further define the nature of alphavirus infection.
MeSH term(s)	Animals ; Arboviruses ; Chikungunya virus ; Cricetinae ; Horses ; Mitochondria ; Sindbis Virus/genetics ; Virus Replication/genetics
Language	English
Publishing date	2022-06-16
Publishing country	Switzerland
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2022.859814
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