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  1. Article ; Online: Progress of research on coronaviruses and toroviruses in large domestic animals using reverse genetics systems.

    Ujike, Makoto / Suzuki, Tohru

    Veterinary journal (London, England : 1997)

    2024  Volume 305, Page(s) 106122

    Abstract: The generation of genetically engineered recombinant viruses from modified DNA/RNA is commonly referred to as reverse genetics, which allows the introduction of desired mutations into the viral genome. Reverse genetics systems (RGSs) are powerful tools ... ...

    Abstract The generation of genetically engineered recombinant viruses from modified DNA/RNA is commonly referred to as reverse genetics, which allows the introduction of desired mutations into the viral genome. Reverse genetics systems (RGSs) are powerful tools for studying fundamental viral processes, mechanisms of infection, pathogenesis and vaccine development. However, establishing RGS for coronaviruses (CoVs) and toroviruses (ToVs), which have the largest genomes among vertebrate RNA viruses, is laborious and hampered by technical constraints. Hence, little research has focused on animal CoVs and ToVs using RGSs, especially in large domestic animals such as pigs and cattle. In the last decade, however, studies of porcine CoVs and bovine ToVs using RGSs have been reported. In addition, the coronavirus disease-2019 pandemic has prompted the development of new and simple CoV RGSs, which will accelerate RGS-based research on animal CoVs and ToVs. In this review, we summarise the general characteristics of CoVs and ToVs, the RGSs available for CoVs and ToVs and the progress made in the last decade in RGS-based research on porcine CoVs and bovine ToVs.
    Language English
    Publishing date 2024-04-17
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 428614-5
    ISSN 1532-2971 ; 0372-5545 ; 1090-0233
    ISSN (online) 1532-2971
    ISSN 0372-5545 ; 1090-0233
    DOI 10.1016/j.tvjl.2024.106122
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 552: Show issues Location:
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  2. Article ; Online: Binaural Listening with Head Rotation Helps Persons with Blindness Perceive Narrow Obstacles.

    Miura, Takahiro / Okochi, Naoyuki / Suzuki, Junya / Ifukube, Tohru

    International journal of environmental research and public health

    2023  Volume 20, Issue 8

    Abstract: Orientation and mobility (O&M) are important abilities that people with visual impairments use in their independent performance of daily activities. In orientation, people with total blindness pinpoint nonsounding objects and sounding objects. The ... ...

    Abstract Orientation and mobility (O&M) are important abilities that people with visual impairments use in their independent performance of daily activities. In orientation, people with total blindness pinpoint nonsounding objects and sounding objects. The ability to perceive nonsounding objects is called
    MeSH term(s) Humans ; Auditory Perception ; Hearing ; Movement ; Blindness
    Language English
    Publishing date 2023-04-19
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2175195-X
    ISSN 1660-4601 ; 1661-7827
    ISSN (online) 1660-4601
    ISSN 1661-7827
    DOI 10.3390/ijerph20085573
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  3. Article ; Online: Class II knotted-like homeodomain protein SlKN5 with BEL1-like homeodomain proteins suppresses fruit greening in tomato fruit.

    Ezura, Kentaro / Lu, Yu / Suzuki, Yutaka / Mitsuda, Nobutaka / Ariizumi, Tohru

    The Plant journal : for cell and molecular biology

    2024  

    Abstract: Knotted1-like homeodomain (KNOX) proteins are essential in regulating plant organ differentiation. Land plants, including tomato (Solanum lycopersicum), have two classes of the KNOX protein family, namely, class I (KNOX I) and class II KNOX (KNOX II). ... ...

    Abstract Knotted1-like homeodomain (KNOX) proteins are essential in regulating plant organ differentiation. Land plants, including tomato (Solanum lycopersicum), have two classes of the KNOX protein family, namely, class I (KNOX I) and class II KNOX (KNOX II). While tomato KNOX I proteins are known to stimulate chloroplast development in fruit, affecting fruit coloration, the role of KNOX II proteins in this context remains unclear. In this study, we employ CRISPR/Cas9 to generate knockout mutants of the KNOX II member, SlKN5. These mutants display increased leaf complexity, a phenotype commonly associated with reduced KNOX II activity, as well as enhanced accumulation of chloroplasts and chlorophylls in smaller cells within young, unripe fruit. RNA-seq data analyses indicate that SlKN5 suppresses the transcriptions of genes involved in chloroplast biogenesis, chlorophyll biosynthesis, and gibberellin catabolism. Furthermore, protein-protein interaction assays reveal that SlKN5 physically interacts with three transcriptional repressors from the BLH1-clade of BEL1-like homeodomain (BLH) protein family, SlBLH4, SlBLH5, and SlBLH7, with SlBLH7 showing the strongest interaction. CRISPR/Cas9-mediated knockout of these SlBLH genes confirmed their overlapping roles in suppressing chloroplast biogenesis, chlorophyll biosynthesis, and lycopene cyclization. Transient assays further demonstrate that the SlKN5-SlBLH7 interaction enhances binding capacity to regulatory regions of key chloroplast- and chlorophyll-related genes, including SlAPRR2-like1, SlCAB-1C, and SlGUN4. Collectively, our findings elucidate that the KNOX II SlKN5-SlBLH regulatory modules serve to inhibit fruit greening and subsequently promote lycopene accumulation, thereby fine-tuning the color transition from immature green fruit to mature red fruit.
    Language English
    Publishing date 2024-04-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 1088037-9
    ISSN 1365-313X ; 0960-7412
    ISSN (online) 1365-313X
    ISSN 0960-7412
    DOI 10.1111/tpj.16727
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    Z 6071: Show issues

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  4. Article: A Japanese school urine screening program led to the diagnosis of

    Hattori, Akito / Okuhara, Koji / Shimizu, Yasuhiro / Ohta, Tohru / Suzuki, Shigeru

    Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology

    2023  Volume 33, Issue 1, Page(s) 12–16

    Abstract: ... ...

    Abstract Although
    Language English
    Publishing date 2023-10-04
    Publishing country Japan
    Document type Case Reports
    ZDB-ID 2079760-6
    ISSN 0918-5739
    ISSN 0918-5739
    DOI 10.1297/cpe.2023-0037
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5710: Show issues Location:
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  5. Article ; Online: Extended Distribution of Relaxation Time Analysis for Electrochemical Impedance Spectroscopy

    Kiyoshi KOBAYASHI / Tohru S. SUZUKI

    Electrochemistry, Vol 90, Iss 1, Pp 017004-

    2022  Volume 017004

    Abstract: The distribution of relaxation time (DRT) is increasingly investigated as a novel analytical method for electrochemical impedance spectroscopy. However, this method has not yet been generalized, as it cannot be applied to a spectrum influenced by an ... ...

    Abstract The distribution of relaxation time (DRT) is increasingly investigated as a novel analytical method for electrochemical impedance spectroscopy. However, this method has not yet been generalized, as it cannot be applied to a spectrum influenced by an isolated capacitator and a resistor connected in parallel with an inductor. To generalize the DRT analysis, we propose a novel principal relation and actual calculation methods using an iterative elastic net regularization algorithm. The elastic net regularization was implemented using the Levenberg–Marquardt method. The proposed algorithm aids in analyzing the DRT for spectra that cannot be solved using conventional methods. We compared the DRT results in different regularization methods obtained from the proposed program with those obtained from other open-source programs. Additionally, the realistic problems of the DRT analysis are discussed considering the calculated results and their theoretical basis. Different DRT curves can be obtained depending on the particular program and regularization methods, even when the spectrum is the same. Moreover, overconfidence in the electrochemical DRT method can be avoided with a clear understanding of DRT basics.
    Keywords extended distribution of relaxation time analysis ; impedance spectroscopy ; elastic net regularization ; spectrum analysis ; Technology ; T ; Physical and theoretical chemistry ; QD450-801
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher The Electrochemical Society of Japan
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Supplementation with cassis polyphenol has no effect on menopausal symptoms in healthy middle-aged women: A randomized, double-blind, parallel-group, placebo-controlled trial.

    Ohtsuka, Mayumi / Yamaguchi, Tohru / Oishi, Sachiko / Misawa, Koichi / Suzuki, Atsushi / Hibi, Masanobu / Terauchi, Masakazu

    Nutrition research (New York, N.Y.)

    2024  Volume 126, Page(s) 14–22

    Abstract: Hormonal changes during the menopause transition may lead to vasomotor symptoms, including hot flashes (HFs) and neuropsychiatric symptoms such as anxiety and irritability. We hypothesized that the effects of cassis polyphenol (CaP) to improve ... ...

    Abstract Hormonal changes during the menopause transition may lead to vasomotor symptoms, including hot flashes (HFs) and neuropsychiatric symptoms such as anxiety and irritability. We hypothesized that the effects of cassis polyphenol (CaP) to improve microcirculation and vasorelaxation may alleviate menopausal symptoms. We performed a randomized, double-blind, parallel-group, placebo-controlled trial involving 59 healthy women (mean [standard deviation] age, 51.3 [4.3] years; body mass index, 20.8 [2.6] kg/m
    Language English
    Publishing date 2024-03-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 582432-1
    ISSN 1879-0739 ; 0271-5317
    ISSN (online) 1879-0739
    ISSN 0271-5317
    DOI 10.1016/j.nutres.2024.03.006
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1686: Show issues Location:
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  7. Article ; Online: Multiplex RT-qPCR Application in Early Detection of Bovine Respiratory Disease in Healthy Calves.

    Goto, Yusuke / Fukunari, Kazuhiro / Suzuki, Tohru

    Viruses

    2023  Volume 15, Issue 3

    Abstract: Bovine respiratory diseases (BRD) are associated with various predisposing factors, such as physical and physiological stress factors, and bacterial and viral pathogens. These stressors and viruses suppress immune defenses, leading to bacterial growth in ...

    Abstract Bovine respiratory diseases (BRD) are associated with various predisposing factors, such as physical and physiological stress factors, and bacterial and viral pathogens. These stressors and viruses suppress immune defenses, leading to bacterial growth in the upper respiratory tract and invasion of pathogens into the lower respiratory tract. Therefore, continuous monitoring of the causative pathogens would contribute to the early detection of BRD. Nasal swabs and sera from 63 clinically healthy calves were continuously collected from seven farms in Iwate prefecture from 2019 to 2021. We attempted to monitor dynamics of BRD-associated pathogens by multiplex real-time RT-PCR (RT-qPCR) using their nasal swab samples. In addition, we attempted to monitor fluctuation of antibody titers against each BRD-associated pathogen by virus neutralization test (VNT) using their sera. In contrast, nasal swabs from 89 calves infected with BRD were collected from 28 farms in Iwate prefecture from 2019 to 2021. We attempted to analyze their nasal swab samples by multiplex RT-qPCR aim to detect BRD-associated pathogens that are dominant in this region. As a result, our analyses using samples from clinically healthy calves showed that positive results by multiplex RT-qPCR were closely related to a significant increase of antibody titers by VNT in bovine coronavirus (BCoV), bovine torovirus (BToV), and bovine respiratory syncytial virus (BRSV). In addition, our data exhibited that BCoV, BToV, BRSV, bovine parainfluenza virus 3, and
    MeSH term(s) Animals ; Cattle ; Cattle Diseases/diagnosis ; Respiratory Tract Diseases/veterinary ; Nose ; Coronavirus, Bovine ; Trachea ; Respiratory Syncytial Virus, Bovine
    Language English
    Publishing date 2023-03-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15030669
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  8. Article ; Online: Structural basis for antiepileptic drugs and botulinum neurotoxin recognition of SV2A.

    Yamagata, Atsushi / Ito, Kaori / Suzuki, Takehiro / Dohmae, Naoshi / Terada, Tohru / Shirouzu, Mikako

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 3027

    Abstract: More than one percent of people have epilepsy worldwide. Levetiracetam (LEV) is a successful new-generation antiepileptic drug (AED), and its derivative, brivaracetam (BRV), shows improved efficacy. Synaptic vesicle glycoprotein 2a (SV2A), a putative ... ...

    Abstract More than one percent of people have epilepsy worldwide. Levetiracetam (LEV) is a successful new-generation antiepileptic drug (AED), and its derivative, brivaracetam (BRV), shows improved efficacy. Synaptic vesicle glycoprotein 2a (SV2A), a putative membrane transporter in the synaptic vesicles (SVs), has been identified as a target of LEV and BRV. SV2A also serves as a receptor for botulinum neurotoxin (BoNT), which is the most toxic protein and has paradoxically emerged as a potent reagent for therapeutic and cosmetic applications. Nevertheless, no structural analysis on AEDs and BoNT recognition by full-length SV2A has been available. Here we describe the cryo-electron microscopy structures of the full-length SV2A in complex with the BoNT receptor-binding domain, BoNT/A2 H
    MeSH term(s) Humans ; Anticonvulsants/metabolism ; Cryoelectron Microscopy ; Levetiracetam/therapeutic use ; Epilepsy/drug therapy ; Botulinum Toxins ; Membrane Glycoproteins/metabolism ; Nerve Tissue Proteins/metabolism
    Chemical Substances Anticonvulsants ; Levetiracetam (44YRR34555) ; Botulinum Toxins (EC 3.4.24.69) ; SV2A protein, human (148845-93-6) ; Membrane Glycoproteins ; Nerve Tissue Proteins
    Language English
    Publishing date 2024-04-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-47322-4
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  9. Article ; Online: Draft Genome Sequence of Bifidobacterium adolescentis 4-2, Isolated from Healthy Human Feces.

    Altaib, Hend / Niwa, Ryo / Abe, Mayuko / Suzuki, Tohru

    Microbiology resource announcements

    2022  Volume 11, Issue 1, Page(s) e0084621

    Abstract: Bifidobacterium adolescentis 4-2 was isolated from healthy human feces. Here, we report a draft genome sequence of this bacterium, which may clarify the functionality of gut microbiota-brain communication. The draft genome comprises 2.39 Mb, with an ... ...

    Abstract Bifidobacterium adolescentis 4-2 was isolated from healthy human feces. Here, we report a draft genome sequence of this bacterium, which may clarify the functionality of gut microbiota-brain communication. The draft genome comprises 2.39 Mb, with an average G+C content of 59.2% and 2,028 coding DNA sequences. An operon for gamma aminobutyric acid (GABA) biosynthesis was observed in the draft genome.
    Language English
    Publishing date 2022-01-13
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/mra.00846-21
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  10. Article ; Online: Short sequence repeats of the intergenic spacer regions of ribosomal RNA genes in Malassezia globosa and Malassezia restricta colonizing the scalps of male individuals with and without androgenetic alopecia.

    Suzuki, Kazuhiro / Cho, Otomi / Nagahama, Tohru / Sugita, Takashi

    Microbiology and immunology

    2022  Volume 66, Issue 6, Page(s) 350–352

    Abstract: We analyzed the short sequence repeats (SSRs) of the intergenic spacer (IGS) region 1 of the ribosomal RNA genes in Malassezia globosa and Malassezia restricta, which predominantly colonize the scalp in androgenetic alopecia (AGA). No AGA-specific SSRs ... ...

    Abstract We analyzed the short sequence repeats (SSRs) of the intergenic spacer (IGS) region 1 of the ribosomal RNA genes in Malassezia globosa and Malassezia restricta, which predominantly colonize the scalp in androgenetic alopecia (AGA). No AGA-specific SSRs were found in the M. globosa IGS region, whereas a (CT)
    MeSH term(s) Alopecia/genetics ; DNA, Intergenic/genetics ; Genes, rRNA ; Humans ; Malassezia/genetics ; Male ; Scalp
    Chemical Substances DNA, Intergenic
    Language English
    Publishing date 2022-04-25
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 224792-6
    ISSN 1348-0421 ; 0385-5600
    ISSN (online) 1348-0421
    ISSN 0385-5600
    DOI 10.1111/1348-0421.12979
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.204: Show issues Location:
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