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  1. Article ; Online: Sequencing the Complete Genome of COVID-19 Virus from Clinical Samples Using the Sanger Method.

    Lu, Roujian / Niu, Peihua / Zhao, Li / Wang, Huijuan / Wang, Wenling / Tan, Wenjie

    China CDC weekly

    2021  Volume 2, Issue 25, Page(s) 447–452

    Language English
    Publishing date 2021-08-29
    Publishing country China
    Document type Journal Article
    ISSN 2096-7071
    ISSN (online) 2096-7071
    DOI 10.46234/ccdcw2020.088
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Characterization of whole genomes from recently emerging Mpox cases in several regions of China, 2023.

    Wu, Changcheng / Cui, Lunbiao / Pan, Yang / Lv, Ziquan / Yao, Mingxiao / Wang, Wenling / Ye, Fei / Huo, Weibang / Zhao, Li / Huang, Baoying / Zhu, Fengcai / Lu, Roujian / Deng, Yao / Wang, Quanyi / Tan, Wenjie

    Science China. Life sciences

    2023  

    Language English
    Publishing date 2023-11-24
    Publishing country China
    Document type Letter
    ZDB-ID 2546732-3
    ISSN 1869-1889 ; 1674-7305
    ISSN (online) 1869-1889
    ISSN 1674-7305
    DOI 10.1007/s11427-023-2485-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Bardoxolone and bardoxolone methyl, two Nrf2 activators in clinical trials, inhibit SARS-CoV-2 replication and its 3C-like protease

    Qi Sun / Fei Ye / Hao Liang / Hongbo Liu / Chunmei Li / Roujian Lu / Baoying Huang / Li Zhao / Wenjie Tan / Luhua Lai

    Signal Transduction and Targeted Therapy, Vol 6, Iss 1, Pp 1-

    2021  Volume 3

    Keywords Medicine ; R ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Development of two multiplex real-time PCR assays for simultaneous detection and differentiation of monkeypox virus IIa, IIb, and I clades and the B.1 lineage

    Shuting Huo / Yuda Chen / Roujian Lu / Zhongxian Zhang / Gaoqian Zhang / Li Zhao / Yao Deng / Changcheng Wu / Wenjie Tan

    Biosafety and Health, Vol 4, Iss 6, Pp 392-

    2022  Volume 398

    Abstract: An ongoing multi-country outbreak of monkeypox was reported in May 2022 with several deaths, affecting 107 countries of all six World Health Organization (WHO) regions. The WHO has declared the current monkeypox outbreak to be a Public Health Emergency ... ...

    Abstract An ongoing multi-country outbreak of monkeypox was reported in May 2022 with several deaths, affecting 107 countries of all six World Health Organization (WHO) regions. The WHO has declared the current monkeypox outbreak to be a Public Health Emergency of International Concern. It is, thus, necessary to rapidly and accurately detect and distinguish different monkeypox virus (MPXV) clades. We designed primers and probes based on the alignment of 138 complete genomes of poxviruses. In Panel 1, we mixed one pair of primers and three probes to detect and differentiate the MPXV Western Africa (IIa, IIb clade) and Congo Basin (I clade) and other orthopoxviruses. In Panel 2, we mixed one pair of primers and two probes to detect the 2022 MPXV (B.1 lineage and its descendant lineages). In addition, we tested the specificity and sensitivity of the assay using real-time PCR. In Panel 1, the assay reproducibly identified various concentrations of two plasmids of the monkeypox virus, whereas other orthopoxviruses did not cross-react. In Panel 2, the probe annealed well to MPXV B.1 and showed the expected linearity. These two multiple real-time assays are inclusive and highly specific for identifying different clades of MPXV.
    Keywords Monkeypox virus ; Monkeypox virus B.1 lineage ; Real-time PCR ; Diagnostic accuracy ; Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
    Subject code 616
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Development of two multiplex real-time PCR assays for simultaneous detection and differentiation of monkeypox virus IIa, IIb, and I clades and the B.1 lineage.

    Huo, Shuting / Chen, Yuda / Lu, Roujian / Zhang, Zhongxian / Zhang, Gaoqian / Zhao, Li / Deng, Yao / Wu, Changcheng / Tan, Wenjie

    Biosafety and health

    2022  

    Abstract: An ongoing multicountry outbreak of monkeypox was reported in May 2022 with several deaths, affecting 107 countries of all six World Health Organization (WHO) regions. The WHO has declared the current monkeypox outbreak a Public Health Emergency of ... ...

    Abstract An ongoing multicountry outbreak of monkeypox was reported in May 2022 with several deaths, affecting 107 countries of all six World Health Organization (WHO) regions. The WHO has declared the current monkeypox outbreak a Public Health Emergency of International Concern. It is, thus, necessary to rapidly and accurately detect and distinguish different monkeypox virus (MPXV) clades. We designed primers and probes based on the alignment of 138 complete genomes of poxviruses. In Panel 1, we mixed one pair of primers and three probes to detect and differentiate the MPXV Western Africa (IIa, IIb clade) and Congo Basin (I clade) and other orthopoxviruses. In Panel 2, we mixed one pair of primers and two probes to detect the 2022 MPXV (B.1 lineage and its descendant lineages). In addition, we tested the specificity and sensitivity of the assay using real-time PCR. In Panel 1, the assay reproducibly identified various concentrations of two plasmids of the monkeypox virus, whereas other orthopoxviruses did not cross-react. In Panel 2, the probe annealed well to MPXV B.1 and showed the expected linearity. These two multiple real-time assays are inclusive and highly specific for identifying different clades of MPXV.
    Language English
    Publishing date 2022-11-04
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2590-0536
    ISSN (online) 2590-0536
    DOI 10.1016/j.bsheal.2022.10.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Detection of SARS-CoV-2 in Different Types of Clinical Specimens.

    Wang, Wenling / Xu, Yanli / Gao, Ruqin / Lu, Roujian / Han, Kai / Wu, Guizhen / Tan, Wenjie

    JAMA

    2020  Volume 323, Issue 18, Page(s) 1843–1844

    MeSH term(s) Adolescent ; Adult ; Aged ; Betacoronavirus/isolation & purification ; Bronchoalveolar Lavage Fluid/virology ; Bronchoscopy/statistics & numerical data ; COVID-19 ; Child ; Child, Preschool ; China ; Coronavirus Infections/blood ; Coronavirus Infections/urine ; Coronavirus Infections/virology ; Feces/virology ; Female ; Gene Dosage ; Genes, Viral ; Humans ; Male ; Middle Aged ; Nose/virology ; Open Reading Frames ; Pandemics ; Pharynx/virology ; Pneumonia, Viral/blood ; Pneumonia, Viral/urine ; Pneumonia, Viral/virology ; RNA, Viral/isolation & purification ; Real-Time Polymerase Chain Reaction ; SARS-CoV-2 ; Sputum/virology ; Viral Load ; Young Adult
    Chemical Substances RNA, Viral
    Keywords covid19
    Language English
    Publishing date 2020-01-27
    Publishing country United States
    Document type Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
    ZDB-ID 2958-0
    ISSN 1538-3598 ; 0254-9077 ; 0002-9955 ; 0098-7484
    ISSN (online) 1538-3598
    ISSN 0254-9077 ; 0002-9955 ; 0098-7484
    DOI 10.1001/jama.2020.3786
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Detecting SARS-CoV-2 BA.2, BA.4, and BA.5 Variants Utilizing a Robust RT-RPA-CRISPR/Cas12a-Based Method - China, 2023.

    Luo, Meihui / Pan, Yang / He, Yaqing / A, Ruhan / Wu, Changcheng / Huang, Baoying / Lu, Roujian / Zhao, Li / Peng, Bo / Ye, Fei / Wang, Huijuan / Chen, Yuda / Li, Zhen / Zhang, Daitao / Wang, Wenling / Tan, Wenjie

    China CDC weekly

    2023  Volume 5, Issue 26, Page(s) 584–591

    Abstract: Introduction: Since 2019, numerous variants of concern for severe acute respiratory syndrome virus 2 (SARS-CoV-2) have emerged, leading to significant outbreaks. The development of novel, highly accurate, and rapid detection techniques for these new ... ...

    Abstract Introduction: Since 2019, numerous variants of concern for severe acute respiratory syndrome virus 2 (SARS-CoV-2) have emerged, leading to significant outbreaks. The development of novel, highly accurate, and rapid detection techniques for these new SARS-CoV-2 variants remains a primary focus in the ongoing efforts to control and prevent the coronavirus disease 2019 (COVID-19) pandemic.
    Methods: Reverse transcription-recombinase polymerase amplification combined with the clustered regularly interspaced short palindromic repeats-associated protein 12a (CRISPR/Cas12a) system was used to validate the detection of the Omicron BA.2, BA.4, and BA.5 variants of SARS-CoV-2.
    Results: Our results demonstrate that the CRISPR/Cas12a assay is capable of effectively detecting the SARS-CoV-2 BA.2, BA.4, and BA.5 variants with a limit of detection of 10, 1, and 10 copies/μL, respectively. Importantly, our assay successfully differentiated the three SARS-CoV-2 Omicron strains from one another. Additionally, we evaluated 46 SARS-CoV-2 positive clinical samples consisting of BA.2 (
    Discussion: This research presents a swift and reliable CRISPR-based method that may be employed to track the emergence of novel SARS-CoV-2 variants.
    Language English
    Publishing date 2023-06-29
    Publishing country China
    Document type Journal Article
    ISSN 2096-7071
    ISSN (online) 2096-7071
    DOI 10.46234/ccdcw2023.113
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Discovery and identification of a novel canine coronavirus causing a diarrhea outbreak in Vulpes.

    Liu, Yuting / Deng, Yao / Niu, Sheng / Zhu, Na / Song, Jingdong / Zhang, Xiaoshuang / Su, Weiting / Nie, Wenhui / Lu, Roujian / Irwin, David M / Gao, George Fu / Wang, Wenling / Wang, Qihui / Tan, Wenjie / Zhang, Shuyi

    Science bulletin

    2023  Volume 68, Issue 21, Page(s) 2598–2606

    Abstract: Cross-species transmission of viruses from wildlife animal reservoirs, such as bats, poses a threat to human and domestic animal health. Previous studies have shown that domestic animals have important roles as intermediate hosts, enabling the ... ...

    Abstract Cross-species transmission of viruses from wildlife animal reservoirs, such as bats, poses a threat to human and domestic animal health. Previous studies have shown that domestic animals have important roles as intermediate hosts, enabling the transmission of genetically diverse coronaviruses from natural hosts to humans. Here, we report the identification and characterization of a novel canine coronavirus (VuCCoV), which caused an epidemic of acute diarrhea in Vulpes (foxes) in Shenyang, China. The epidemic started on November 8, 2019, and caused more than 39,600 deaths by January 1, 2022. Full-length viral genomic sequences were obtained from 15 foxes with diarrhea at the early stage of this outbreak. The VuCCoV genome shared more than 90% nucleotide identity with canine coronavirus (CCoV) for three of the four structural genes, with the S gene showing a larger amount of divergence. In addition, 67% (10/15) of the VuCCoV genomes contained an open reading frame (ORF3) gene, which was previously only detected in CCoV-I genomes. Notably, VuCCoV had only two to three amino acid differences at the partial RNA-dependent RNA polymerase (RdRp) level to bat CoV, suggesting a close genetic relationship. Therefore, these novel VuCCoV genomes represent a previously unsampled lineage of CCoVs. We also show that the VuCCoV spike protein binds to canine and fox aminopeptidase N (APN), which may allow this protein to serve as an entry receptor. In addition, cell lines were identified that are sensitive to VuCCoV using a pseudovirus system. These data highlight the importance of identifying the diversity and distribution of coronaviruses in domestic animals, which could mitigate future outbreaks that could threaten livestock, public health, and economic growth.
    MeSH term(s) Animals ; Dogs ; Humans ; Foxes ; Coronavirus, Canine/genetics ; Animals, Wild ; SARS-CoV-2/genetics ; Animals, Domestic ; Disease Outbreaks/veterinary ; Diarrhea/epidemiology
    Language English
    Publishing date 2023-09-12
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2816140-3
    ISSN 2095-9281 ; 2095-9273
    ISSN (online) 2095-9281
    ISSN 2095-9273
    DOI 10.1016/j.scib.2023.09.011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Etiology of Severe Pneumonia in Children in Alveolar Lavage Fluid Using a High-Throughput Gene Targeted Amplicon Sequencing Assay.

    Li, Fei / Wang, Yin / Zhang, Yuhan / Shi, Peng / Cao, Linfeng / Su, LiYun / Zhu, Qiguo / Wang, Libo / Lu, Roujian / Tan, Wenjie / Shen, Jun

    Frontiers in pediatrics

    2021  Volume 9, Page(s) 659164

    Abstract: Objective: ...

    Abstract Objective:
    Language English
    Publishing date 2021-06-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2711999-3
    ISSN 2296-2360
    ISSN 2296-2360
    DOI 10.3389/fped.2021.659164
    Database MEDical Literature Analysis and Retrieval System OnLINE

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