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Article ; Online: Exonic sequencing and MLH3 gene expression analysis of breast cancer patients.

Khailany, Rozhgar A / Ozaslan, Mehmet

Cellular and molecular biology (Noisy-le-Grand, France)

2021 Volume 67, Issue 3, Page(s) 35–43

Abstract: Breast cancer is the most common cancer in women worldwide. Detection of breast cancer susceptibility genes is an important issue. Also, MLH3 is a DNA mismatch repair gene and mutation in this gene is harmful in different cancers. This study aimed to use ...

Abstract	Breast cancer is the most common cancer in women worldwide. Detection of breast cancer susceptibility genes is an important issue. Also, MLH3 is a DNA mismatch repair gene and mutation in this gene is harmful in different cancers. This study aimed to use exome sequencing to uncover previously undetected breast cancer-predisposing variants. Also, we investigated the MLH3 gene expression of breast cancer patients which can be a breast cancer susceptibility gene. A total of 80 samples including 40 paired normal and cancer tissue samples were collected at Zheen International Hospital, Erbil, Iraq. Exome sequencing was used to identify mutations. Different in silico tools were used to predict the effect of mutation on the structural features or protein function. Real-time PCR was used for assessing the expression of MLH3 in breast cancer patients. We identified 26 variants in breast cancer patients, 22 inherited variants were found in MLH3, CHECK2, BRCA1, BRCA2, BLM, TP53, MSH6, NBN and PTEN genes and 4 somatic variants were found in PALB2, RAD50 and RBM10 genes. It was found that the expression of the MLH3 gene in tumor samples was significantly down-regulated compared with normal tissues. Statistically, high significance was found. The decreased expression of MLH3 was significant in all ranges of ages and all breast cancer types. Also, the expression of MLH3 decreased significantly in patients with breast cancer grades of II and III. In conclusion, MLH3 can be used as a susceptibility gene especially in grades II and III of breast cancer.
MeSH term(s)	Adult ; BRCA1 Protein/genetics ; BRCA2 Protein/genetics ; Breast Neoplasms/genetics ; Breast Neoplasms/pathology ; Fanconi Anemia Complementation Group N Protein/genetics ; Female ; Gene Expression Regulation, Neoplastic ; Genetic Predisposition to Disease/genetics ; Humans ; Middle Aged ; MutL Proteins/genetics ; Mutation ; Neoplasm Grading ; RecQ Helicases/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Whole Exome Sequencing/methods
Chemical Substances	BRCA1 Protein ; BRCA1 protein, human ; BRCA2 Protein ; BRCA2 protein, human ; Fanconi Anemia Complementation Group N Protein ; MLH3 protein, human ; PALB2 protein, human ; Bloom syndrome protein (EC 3.6.1.-) ; MutL Proteins (EC 3.6.1.3) ; RecQ Helicases (EC 3.6.4.12)
Language	English
Publishing date	2021-11-25
Publishing country	France
Document type	Journal Article
ZDB-ID	1161779-2
ISSN	1165-158X ; 0145-5680
ISSN (online)	1165-158X
ISSN	0145-5680
DOI	10.14715/cmb/2021.67.3.5
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Genomic characterization of a novel SARS-CoV-2.

Khailany, Rozhgar A / Safdar, Muhamad / Ozaslan, Mehmet

Gene reports

2020 Volume 19, Page(s) 100682

Abstract: A new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) associated with human to human transmission and extreme human sickness has been as of late announced from the city of Wuhan in China. Our objectives were to mutation analysis between ... ...

Abstract	A new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) associated with human to human transmission and extreme human sickness has been as of late announced from the city of Wuhan in China. Our objectives were to mutation analysis between recently reported genomes at various times and locations and to characterize the genomic structure of SARS-CoV-2 using bioinformatics programs. Information on the variation of viruses is of considerable medical and biological impacts on the prevention, diagnosis, and therapy of infectious diseases. To understand the genomic structure and variations of the SARS-CoV-2. The study analyzed 95 SARS-CoV-2 complete genome sequences available in GenBank, National MicrobiologyData Center (NMDC) and NGDC Genome Warehouse from December-2019 until 05 of April-2020. The genomic signature analysis demonstrates that a strong association between the time of sample collection, location of sample and accumulation of genetic diversity. We found 116 mutations, the three most common mutations were 8782C>T in ORF1ab gene, 28144T>C in ORF8 gene and 29095C>T in the N gene. The mutations might affect the severity and spread of the SARS-CoV-2. The finding heavily supports an intense requirement for additional prompt, inclusive investigations that combine genomic detail, epidemiological information and graph records of the clinical features of patients with COVID-19.
Keywords	covid19
Language	English
Publishing date	2020-04-16
Publishing country	United States
Document type	Journal Article
ISSN	2452-0144
ISSN	2452-0144
DOI	10.1016/j.genrep.2020.100682
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Article ; Online: A Novel β

Atroshi, Sulav D / Al-Allawi, Nasir / Chui, David H K / Najmabadi, Hossein / Khailany, Rozhgar A

Hemoglobin

2021 Volume 45, Issue 3, Page(s) 212–214

Abstract: We report a novel frameshift β-thalassemia (β-thal) mutation due to a two-nucleotide deletion at codon 118 of the β-globin gene ( ...

Abstract	We report a novel frameshift β-thalassemia (β-thal) mutation due to a two-nucleotide deletion at codon 118 of the β-globin gene (
MeSH term(s)	Child, Preschool ; Codon ; Female ; Frameshift Mutation ; Humans ; Iraq ; beta-Globins/genetics ; beta-Thalassemia/diagnosis ; beta-Thalassemia/ethnology ; beta-Thalassemia/genetics
Chemical Substances	Codon ; beta-Globins
Language	English
Publishing date	2021-06-24
Publishing country	England
Document type	Journal Article
ZDB-ID	750615-6
ISSN	1532-432X ; 0363-0269
ISSN (online)	1532-432X
ISSN	0363-0269
DOI	10.1080/03630269.2021.1941082
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Article ; Online: Genomic characterization of a novel SARS-CoV-2

Khailany, Rozhgar A. / Safdar, Muhamad / Ozaslan, Mehmet

Gene Reports

2020 Volume 19, Page(s) 100682

Keywords	covid19
Language	English
Publisher	Elsevier BV
Publishing country	us
Document type	Article ; Online
ISSN	2452-0144
DOI	10.1016/j.genrep.2020.100682
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: KRAS and MT-CO1 genes in colorectal cancer: a molecular investigation.

A Hama, Harmand / Hasan, Bestoon S / Barzngy, Basak / Abdulla, Saman S / Karim, Abdulkarim Y / Khailany, Rozhgar A / Miasko, Monika / Dabrowski, Janusz M / Pucelik, Barbara

Cellular and molecular biology (Noisy-le-Grand, France)

2023 Volume 69, Issue 11, Page(s) 30–35

Abstract: Colorectal cancer (CRC) is the third leading cause of cancer-related deaths worldwide. The tumor suppressor gene MT-CO1, and Kristen Rat Sarcoma Virus (KRAS), an oncogene are primarily responsible for controlling cell apoptosis, cell cycle arrest, and ... ...

Abstract	Colorectal cancer (CRC) is the third leading cause of cancer-related deaths worldwide. The tumor suppressor gene MT-CO1, and Kristen Rat Sarcoma Virus (KRAS), an oncogene are primarily responsible for controlling cell apoptosis, cell cycle arrest, and cell proliferation, and any irregularities in these genes could lead to cancer. This study aims to examine the expression of KRAS and MT-CO1 in CRC biopsy specimens and investigate their relationship with one another in CRC patients residing in the Erbil city of Kurdistan Region, Iraq. The study involved categorizing 42 sets of colorectal cancer tissues and their corresponding controls based on their types and patients' clinical characteristics. The expression of KRAS and MT-CO1 in the samples was assessed using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), with statistical significance set at p<0.05. The expression of KRAS was found to be significantly higher in CRC compared to the control (n=42, p=0.0001). On the other hand, the expression of MT-CO1 did not exhibit significant differences compared to the control group with a p-value of 0.12. Furthermore, the Chi-square and correlation analysis results depicted that MT-CO1 expression negatively correlates with KRAS expression (p= 0.0001, r= -0.047) in CRC tissues. In conclusion, the variation in the expression of KRAS and MT-CO1, and their correlations could potentially serve as a good indicator in the detection and prognosis of CRC, which might lead to better translational research on the same. However, for a better understanding of the underlying mechanisms, further analysis is required.
MeSH term(s)	Humans ; Proto-Oncogene Proteins p21(ras)/genetics ; Oncogenes ; Biopsy ; Apoptosis ; Colorectal Neoplasms/genetics
Chemical Substances	Proto-Oncogene Proteins p21(ras) (EC 3.6.5.2) ; KRAS protein, human
Language	English
Publishing date	2023-11-15
Publishing country	France
Document type	Journal Article
ZDB-ID	1161779-2
ISSN	1165-158X ; 0145-5680
ISSN (online)	1165-158X
ISSN	0145-5680
DOI	10.14715/cmb/2023.69.11.5
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Genetic biomarkers: Potential roles in cancer diagnosis.

Khailany, Rozhgar A / Aziz, Sherwan Ahmed / Najjar, Suhel Mawlood / Safdar, Muhammad / Ozaslan, Mehmet

Cellular and molecular biology (Noisy-le-Grand, France)

2020 Volume 66, Issue 3, Page(s) 1–7

Abstract: Biomarkers are indicators of pathogenic processes, typical biological processes, or pharmacological reactions to a therapy. It has several potential usages in cancer; differential diagnosis, prognosis, risk assessment, therapeutic response, and ... ...

Abstract	Biomarkers are indicators of pathogenic processes, typical biological processes, or pharmacological reactions to a therapy. It has several potential usages in cancer; differential diagnosis, prognosis, risk assessment, therapeutic response, and monitoring of disease progression. Recently, advances in oncomarkers raised significant opportunities for enhancing management of cancer. Chromosomal aberration, molecular impairment and epigenetic alteration might be applied to diagnose and prognose cancer and its epidemiology. Some oncomarkers are specific and highly sensitive for detection. An oncomarker might be used to see how the body reacts to an intervention or a situation. The present study represents a short review about various genetic oncomarkers with diagnostic and prognostic values.
MeSH term(s)	Biomarkers, Tumor/genetics ; Epigenesis, Genetic ; Genetic Markers ; Humans ; Neoplasms/diagnosis ; Neoplasms/genetics ; RNA, Neoplasm/genetics ; RNA, Neoplasm/metabolism
Chemical Substances	Biomarkers, Tumor ; Genetic Markers ; RNA, Neoplasm
Language	English
Publishing date	2020-06-05
Publishing country	France
Document type	Journal Article ; Review
ZDB-ID	1161779-2
ISSN	1165-158X ; 0145-5680
ISSN (online)	1165-158X
ISSN	0145-5680
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Genomic characterization of a novel SARS-CoV-2

Khailany, Rozhgar A / Safdar, Muhamad / Ozaslan, Mehmet

Gene Rep

Abstract	A new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) associated with human to human transmission and extreme human sickness has been as of late announced from the city of Wuhan in China. Our objectives were to mutation analysis between recently reported genomes at various times and locations and to characterize the genomic structure of SARS-CoV-2 using bioinformatics programs. Information on the variation of viruses is of considerable medical and biological impacts on the prevention, diagnosis, and therapy of infectious diseases. To understand the genomic structure and variations of the SARS-CoV-2. The study analyzed 95 SARS-CoV-2 complete genome sequences available in GenBank, National MicrobiologyData Center (NMDC) and NGDC Genome Warehouse from December-2019 until 05 of April-2020. The genomic signature analysis demonstrates that a strong association between the time of sample collection, location of sample and accumulation of genetic diversity. We found 116 mutations, the three most common mutations were 8782C>T in ORF1ab gene, 28144T>C in ORF8 gene and 29095C>T in the N gene. The mutations might affect the severity and spread of the SARS-CoV-2. The finding heavily supports an intense requirement for additional prompt, inclusive investigations that combine genomic detail, epidemiological information and graph records of the clinical features of patients with COVID-19.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #32300673
Database	COVID19

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Article ; Online: Expression patterns of LncRNA-GAS5 and its target APOBEC3C gene through miR-103 in breast cancer patients.

Kanabe, Belan O / Ozaslan, Mehmet / Aziz, Sherwan Ahmed / Al-Attar, Mustafa S / Kılıç, İbrahim Halil / Khailany, Rozhgar A

Cellular and molecular biology (Noisy-le-Grand, France)

2021 Volume 67, Issue 3, Page(s) 5–10

Abstract: Early diagnosis of breast cancer can increase the survivability of the patients and the patient's quality of life. There is growing evidence demonstrating the active role of LncRNA-GAS5 and miR-103 in cancer biology. APOBEC enzymes are important players ... ...

Abstract	Early diagnosis of breast cancer can increase the survivability of the patients and the patient's quality of life. There is growing evidence demonstrating the active role of LncRNA-GAS5 and miR-103 in cancer biology. APOBEC enzymes are important players in immunity and may contribute to carcinogenesis. Mutation and expression alteration in the APOBEC gene family was found to have a strong correlation with breast cancer risk. This study aimed to evaluate the expression level of lncRNA-GAS5 and its target APOBEC3C in women with breast cancer through expression evaluation of miR-103. Moreover, the interaction between lncRNA-GAS5 and miR-103 was studied. In the present study, forty paired tumor and normal samples classified based on breast cancer subtypes and clinical features of patients were analyzed using gene expression studies. Immunohistochemical analysis of the gene products was performed to classify tumors. The RNA samples were extracted from breast tissue. Real-time PCR was conducted for APOBEC3C and Lnc-RNA GAS5 expression. In addition, miR-103a miScript Primer Assay was utilized for the expression of miR-103-5p. It was revealed that the expression level of APOBEC3C and lncRNA-GAS5 were significantly down-regulated; however, the miRNA-103 expression level was significantly up-regulated. GAS5 expression was positively correlated with APOBEC3C expression and negatively correlated with miR-103 expression. In conclusion, we observed down-regulation of APOBEC3C and LncRNA-GAS5 and up-regulation of miRNA 103 in breast cancer patients. The expression of GAS5 may provide a new potential treatment target for breast cancer. To clarify the role of these molecules in the cellular signaling pathways, further studies are required.
MeSH term(s)	Breast Neoplasms/diagnosis ; Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Cytidine Deaminase/genetics ; Cytidine Deaminase/metabolism ; Female ; Gene Expression Profiling/methods ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs/genetics ; Prognosis ; RNA, Long Noncoding/genetics ; Reverse Transcriptase Polymerase Chain Reaction
Chemical Substances	GAS5 long non-coding RNA, human ; MIRN103 microRNA, human ; MicroRNAs ; RNA, Long Noncoding ; APOBEC3C protein, human (EC 3.5.4.5) ; Cytidine Deaminase (EC 3.5.4.5)
Language	English
Publishing date	2021-11-25
Publishing country	France
Document type	Journal Article
ZDB-ID	1161779-2
ISSN	1165-158X ; 0145-5680
ISSN (online)	1165-158X
ISSN	0145-5680
DOI	10.14715/cmb/2021.67.3.2
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Practical measures to prevent COVID-19: A mini-review

Ozaslan, Mehmet / Safdar, Muhamad / Halil Kilic, I. / Khailany, Rozhgar A.

J. Biol. Sci.

Abstract: In view of the global COVID-19 pandemic, this study focuses on the most up-to-date epidemiology, transmission, clinical manifestations, effective management, prevention and maintaining social distancing worldwide. The current outbreak of severe acute ... ...

Abstract	In view of the global COVID-19 pandemic, this study focuses on the most up-to-date epidemiology, transmission, clinical manifestations, effective management, prevention and maintaining social distancing worldwide. The current outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19 disease was first reported from Wuhan, China, in December, 2019. Till now this pandemic had spread to 209 countries and territories around the world and 2 international conveyances with 1,455,519 confirmed cases, including 83,664 deaths, as of April 08, 2020, so the World Health Organization declared it as a Public Health Emergency of worldwide (https://www.worldometers.info/coronavirus/). The main clinical manifestations are included fever, coughing, sore throat, fatigue, headache, diarrhea, hemoptysis, trouble breathing, blue lips or face, persistent pain or pressure in the chest, confusion and excessive drowsiness. Preventive measures such as; masks, frequent hand washing, staying at home, avoid public contact and quarantines are being recommended for reducing the transmission. To date, no specific antiviral treatment is proven yet. The countries with a high risk of infection, they need proper awareness and management for good protection.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #208478
Database	COVID19

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Article: Novel SARS-CoV-2/COVID-19: Origin, pathogenesis, genes and genetic variations, immune responses and phylogenetic analysis.

Junejo, Yasmeen / Ozaslan, Mehmet / Safdar, Muhamad / Khailany, Rozhgar A / Rehman, SaifUr / Yousaf, Wasim / Khan, Musarrat Abbas

Gene reports

2020 Volume 20, Page(s) 100752

Abstract: In this review, we focused on the origins of the novel coronavirus (SARS-CoV-2), origin, pathogenesis, immune responses, genes and genetic variations, phylogenetic analyses, and potential therapeutic strategies to summarize approaches for developing ... ...

Abstract	In this review, we focused on the origins of the novel coronavirus (SARS-CoV-2), origin, pathogenesis, immune responses, genes and genetic variations, phylogenetic analyses, and potential therapeutic strategies to summarize approaches for developing broadly effective preventions and vaccines to cope COVID-19. Towards the end of 2019, SARS-CoV-2 has emerged in association with the SARS, later was named COVID-19 caused an environment of chaos worldwide and infected a massive number of lives. Since these epidemics or pandemics had spread to 210 countries and territories around the world and 2 international conveyances with 6,467,229 confirmed cases, including, 382,766 deaths, as of June 03, 2020 (https://www.worldometers.info/coronavirus/), hence the World Health Organization declared it as a global Public Health Emergency. There are no clinically approved vaccines or antiviral drugs available for either of new or old corona infections; thus, the development of effective therapeutic and preventive strategies that can be readily available to cope with these strains.
Keywords	covid19
Language	English
Publishing date	2020-06-15
Publishing country	United States
Document type	Journal Article
ISSN	2452-0144
ISSN	2452-0144
DOI	10.1016/j.genrep.2020.100752
Database	MEDical Literature Analysis and Retrieval System OnLINE

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