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  1. Article ; Online: Finding antigens for TB vaccines: the good, the bad and the useless.

    Ogongo, Paul / Ernst, Joel D

    Nature medicine

    2023  Volume 29, Issue 1, Page(s) 35–36

    MeSH term(s) Humans ; Vaccines ; Tuberculosis/epidemiology ; Tuberculosis/prevention & control ; Tuberculosis Vaccines ; Mycobacterium tuberculosis
    Chemical Substances Vaccines ; Tuberculosis Vaccines
    Language English
    Publishing date 2023-01-06
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Comment
    ZDB-ID 1220066-9
    ISSN 1546-170X ; 1078-8956
    ISSN (online) 1546-170X
    ISSN 1078-8956
    DOI 10.1038/s41591-022-02123-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Book: Phagocytosis of bacteria and bacterial pathogenicity

    Ernst, Joel D.

    (Advances in molecular and cellular microbiology ; 12)

    2006  

    Author's details ed. by Joel D. Ernst
    Series title Advances in molecular and cellular microbiology ; 12
    Collection
    Language English
    Size IX, 285 S. : Ill., graph. Darst.
    Publisher Cambridge Univ. Press
    Publishing place Cambridge u.a.
    Publishing country Great Britain
    Document type Book
    HBZ-ID HT014652232
    ISBN 978-0-521-84569-4 ; 0-521-84569-6
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    2006 A 4570 order for loan Magazin

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  3. Article ; Online: Circularization of

    Limberis, Jason D / Nalyvayko, Alina / Ernst, Joel D / Metcalfe, John Z

    Microbiology spectrum

    2023  , Page(s) e0412722

    Abstract: Circular DNA offers benefits over linear DNA in diagnostic and field assays, but currently, circular DNA generation is lengthy, inefficient, highly dependent on the length and sequence of DNA, and can result in unwanted chimeras. We present streamlined ... ...

    Abstract Circular DNA offers benefits over linear DNA in diagnostic and field assays, but currently, circular DNA generation is lengthy, inefficient, highly dependent on the length and sequence of DNA, and can result in unwanted chimeras. We present streamlined methods for generating PCR-targeted circular DNA from a 700 bp amplicon of
    Language English
    Publishing date 2023-03-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.04127-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A simple, single-tube overlapping amplicon-targeted Illumina sequencing assay.

    Limberis, Jason D / Nalyvayko, Alina / Ernst, Joel D / Metcalfe, John Z

    PloS one

    2023  Volume 18, Issue 9, Page(s) e0288687

    Abstract: Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications require ...

    Abstract Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications require the splitting of limited clinical samples between two reactions. Here, we outline hairpin Illumina single-tube sequencing PCR (hissPCR) which allows for the generation of overlapping amplicons containing Illumina indexes and adapters in a single tube, effectively extending the Illumina read length while maintaining reagent and sample input requirements.
    MeSH term(s) High-Throughput Nucleotide Sequencing ; Biological Assay ; Mutation ; Polymerase Chain Reaction
    Language English
    Publishing date 2023-09-14
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0288687
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing.

    Limberis, Jason D / Nalyvayko, Alina / Ernst, Joel D / Metcalfe, John Z

    PloS one

    2023  Volume 18, Issue 11, Page(s) e0289442

    Abstract: Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs are long non-coding RNAs that ... ...

    Abstract Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs are long non-coding RNAs that perform enhancer-like functions, are bi-directionally transcribed, and usually lack polyA tails. This paper presents a novel method, selSeq, that selectively removes mRNA and pre-mRNA from samples enabling the selective sequencing of crucial regulatory elements, including non-polyadenylated RNAs such as long non-coding RNA, enhancer RNA, and non-canonical mRNA.
    MeSH term(s) RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; Gene Expression Regulation ; RNA, Messenger/genetics ; Transcriptome ; RNA Precursors
    Chemical Substances RNA, Long Noncoding ; RNA, Messenger ; RNA Precursors
    Language English
    Publishing date 2023-11-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0289442
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Float Like Bacilli, STING Like a B: Type I Interferons in Tuberculosis.

    Ernst, Joel D

    American journal of respiratory and critical care medicine

    2017  Volume 197, Issue 6, Page(s) 706–707

    MeSH term(s) B-Lymphocytes ; Humans ; Interferon Type I ; Macrophages ; Tuberculosis
    Chemical Substances Interferon Type I
    Language English
    Publishing date 2017-12-20
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 1180953-x
    ISSN 1535-4970 ; 0003-0805 ; 1073-449X
    ISSN (online) 1535-4970
    ISSN 0003-0805 ; 1073-449X
    DOI 10.1164/rccm.201712-2368ED
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  7. Article ; Online: Mechanisms of M. tuberculosis Immune Evasion as Challenges to TB Vaccine Design.

    Ernst, Joel D

    Cell host & microbe

    2018  Volume 24, Issue 1, Page(s) 34–42

    Abstract: Tuberculosis (TB) is a large global health problem, in part because of the long period of coevolution of the pathogen, Mycobacterium tuberculosis, and its human host. A major factor that sustains the global epidemic of TB is the lack of a sufficiently ... ...

    Abstract Tuberculosis (TB) is a large global health problem, in part because of the long period of coevolution of the pathogen, Mycobacterium tuberculosis, and its human host. A major factor that sustains the global epidemic of TB is the lack of a sufficiently effective vaccine. While basic mechanisms of immunity that protect against TB have been identified, attempts to improve immunity to TB by vaccination have been disappointing. This Review discusses the mechanisms used by M. tuberculosis to evade innate and adaptive immunity and that likely limit the efficacy of vaccines developed to date. Despite multiple mechanisms of immune evasion, recent trials have indicated that effective TB vaccines remain an attainable goal. This Review discusses how knowledge from other systems can inform improvements on current vaccine approaches.
    MeSH term(s) Adaptive Immunity/immunology ; Animals ; BCG Vaccine/immunology ; Clinical Trials as Topic ; Granuloma/immunology ; Granuloma/microbiology ; Host-Pathogen Interactions/immunology ; Host-Pathogen Interactions/physiology ; Humans ; Immune Evasion/immunology ; Immunity, Innate/immunology ; Mycobacterium tuberculosis/immunology ; Mycobacterium tuberculosis/physiology ; T-Lymphocytes/immunology ; T-Lymphocytes/microbiology ; Tuberculosis/immunology ; Tuberculosis/microbiology ; Tuberculosis/prevention & control ; Tuberculosis Vaccines/immunology ; Vaccines, Attenuated/immunology
    Chemical Substances BCG Vaccine ; Tuberculosis Vaccines ; Vaccines, Attenuated
    Language English
    Publishing date 2018-07-12
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2278004-X
    ISSN 1934-6069 ; 1931-3128
    ISSN (online) 1934-6069
    ISSN 1931-3128
    DOI 10.1016/j.chom.2018.06.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6578: Show issues Location:
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  8. Article: Antigenic Variation and Immune Escape in the MTBC.

    Ernst, Joel D

    Advances in experimental medicine and biology

    2017  Volume 1019, Page(s) 171–190

    Abstract: Microbes that infect other organisms encounter host immune responses, and must overcome or evade innate and adaptive immune responses to successfully establish infection. Highly successful microbial pathogens, including M. tuberculosis, are able to evade ...

    Abstract Microbes that infect other organisms encounter host immune responses, and must overcome or evade innate and adaptive immune responses to successfully establish infection. Highly successful microbial pathogens, including M. tuberculosis, are able to evade adaptive immune responses (mediated by antibodies and/or T lymphocytes) and thereby establish long-term chronic infection. One mechanism that diverse pathogens use to evade adaptive immunity is antigenic variation, in which structural variants emerge that alter recognition by established immune responses and allow those pathogens to persist and/or to infect previously-immune hosts. Despite the wide use of antigenic variation by diverse pathogens, this mechanism appears to be infrequent in M. tuberculosis, as indicated by findings that known and predicted human T cell epitopes in this organism are highly conserved, although there are exceptions. These findings have implications for diagnostic tests that are based on measuring host immune responses, and for vaccine design and development.
    MeSH term(s) Adaptive Immunity ; Antigenic Variation ; Antigens, Bacterial/genetics ; Antigens, Bacterial/immunology ; Conserved Sequence ; Epitopes, T-Lymphocyte/genetics ; Epitopes, T-Lymphocyte/immunology ; Gene Expression Regulation ; Genome, Bacterial ; HLA Antigens/genetics ; HLA Antigens/immunology ; Humans ; Immune Evasion ; Immunity, Innate ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/growth & development ; Mycobacterium tuberculosis/immunology ; Selection, Genetic ; T-Lymphocytes/immunology ; T-Lymphocytes/microbiology ; Tuberculosis/genetics ; Tuberculosis/immunology ; Tuberculosis/microbiology
    Chemical Substances Antigens, Bacterial ; Epitopes, T-Lymphocyte ; HLA Antigens
    Language English
    Publishing date 2017-11-07
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2214-8019 ; 0065-2598
    ISSN (online) 2214-8019
    ISSN 0065-2598
    DOI 10.1007/978-3-319-64371-7_9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: selSeq

    Jason D Limberis / Alina Nalyvayko / Joel D Ernst / John Z Metcalfe

    PLoS ONE, Vol 18, Iss 11, p e

    A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing.

    2023  Volume 0289442

    Abstract: Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs are long non-coding RNAs that ... ...

    Abstract Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs are long non-coding RNAs that perform enhancer-like functions, are bi-directionally transcribed, and usually lack polyA tails. This paper presents a novel method, selSeq, that selectively removes mRNA and pre-mRNA from samples enabling the selective sequencing of crucial regulatory elements, including non-polyadenylated RNAs such as long non-coding RNA, enhancer RNA, and non-canonical mRNA.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  10. Article ; Online: A teenage girl with altered mental status and paraparesis.

    Miyakawa, Ryo / Louie, Janice / Keh, Chris / Chen, Lisa / Javid, Babak / Ernst, Joel D / Goswami, Neela / Chow, Felicia C

    Journal of clinical tuberculosis and other mycobacterial diseases

    2024  Volume 35, Page(s) 100425

    Abstract: A teenage girl presented with fever and altered mental status. MRI showed diffuse leptomeningeal enhancement of the brain and spine. She was diagnosed by a positive cerebrospinal fluid (CSF) culture with tuberculous (TB) meningitis and was started on ... ...

    Abstract A teenage girl presented with fever and altered mental status. MRI showed diffuse leptomeningeal enhancement of the brain and spine. She was diagnosed by a positive cerebrospinal fluid (CSF) culture with tuberculous (TB) meningitis and was started on anti-TB medications and corticosteroids. Her mental status improved, but she was noted to have proximal weakness of the lower extremities. In the course of tapering corticosteroids at week 11 of anti-TB therapy, she became acutely confused and febrile. MRI demonstrated interval development of tuberculomas in the brain and a mass lesion in the thoracic spine causing cord compression. Given the clinical picture was suggestive of a paradoxical reaction, the dose of corticosteroids was increased. Infliximab was added when repeat MRI revealed enlargement of the mass lesion in the spine with worsening cord compression. She was successfully tapered off of corticosteroids. Over several months, the patient's motor function recovered fully, and she returned to ambulating without assistance.
    Language English
    Publishing date 2024-03-01
    Publishing country England
    Document type Case Reports
    ISSN 2405-5794
    ISSN (online) 2405-5794
    DOI 10.1016/j.jctube.2024.100425
    Database MEDical Literature Analysis and Retrieval System OnLINE

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