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  1. Article ; Online: Giant cell formation and function.

    Brodbeck, William G / Anderson, James M

    Current opinion in hematology

    2008  Volume 16, Issue 1, Page(s) 53–57

    Abstract: Purpose of review: To provide insight into the current state of understanding regarding the molecular and cellular mechanisms underlying the formation and function of various types of multinucleated giant cells.: Recent findings: Recent studies ... ...

    Abstract Purpose of review: To provide insight into the current state of understanding regarding the molecular and cellular mechanisms underlying the formation and function of various types of multinucleated giant cells.
    Recent findings: Recent studies involving mainly osteoclasts and foreign body giant cells have revealed a number of common factors, for example, vitronectin, an adhesion protein, dendritic cell-specific transmembrane protein, a fusion factor, and macrophage fusion receptor, that contribute to giant cell formation and function. Insight into common molecules, receptors, and mediators of adhesion and fusion mechanisms of giant cell formation have been complicated by the wide diversity of species, models, and cell types utilized in these studies.
    Summary: These recently identified factors together with the well known osteoclast receptor, alphavbeta3, may serve as potential therapeutic targets for the modulation and inhibition of multinucleated giant cell formation and function. Further studies on intracellular and intercellular signaling mechanisms modulating multinucleated giant cell formation and function are necessary for the identification of therapeutic targets as well as a better understanding of giant cell biology.
    MeSH term(s) Cell Adhesion ; Cell Fusion ; Drug Delivery Systems ; Giant Cells/pathology ; Giant Cells/physiology ; Giant Cells, Foreign-Body/pathology ; Humans ; Integrin alphaVbeta3 ; Osteoclasts/pathology
    Chemical Substances Integrin alphaVbeta3
    Language English
    Publishing date 2008-12-04
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 1153887-9
    ISSN 1531-7048 ; 1065-6251
    ISSN (online) 1531-7048
    ISSN 1065-6251
    DOI 10.1097/MOH.0b013e32831ac52e
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  2. Article ; Online: Tumor-selective activity of RAS-GTP inhibition in pancreatic cancer.

    Wasko, Urszula N / Jiang, Jingjing / Dalton, Tanner C / Curiel-Garcia, Alvaro / Edwards, A Cole / Wang, Yingyun / Lee, Bianca / Orlen, Margo / Tian, Sha / Stalnecker, Clint A / Drizyte-Miller, Kristina / Menard, Marie / Dilly, Julien / Sastra, Stephen A / Palermo, Carmine F / Hasselluhn, Marie C / Decker-Farrell, Amanda R / Chang, Stephanie / Jiang, Lingyan /
    Wei, Xing / Yang, Yu C / Helland, Ciara / Courtney, Haley / Gindin, Yevgeniy / Muonio, Karl / Zhao, Ruiping / Kemp, Samantha B / Clendenin, Cynthia / Sor, Rina / Vostrejs, William P / Hibshman, Priya S / Amparo, Amber M / Hennessey, Connor / Rees, Matthew G / Ronan, Melissa M / Roth, Jennifer A / Brodbeck, Jens / Tomassoni, Lorenzo / Bakir, Basil / Socci, Nicholas D / Herring, Laura E / Barker, Natalie K / Wang, Junning / Cleary, James M / Wolpin, Brian M / Chabot, John A / Kluger, Michael D / Manji, Gulam A / Tsai, Kenneth Y / Sekulic, Miroslav / Lagana, Stephen M / Califano, Andrea / Quintana, Elsa / Wang, Zhengping / Smith, Jacqueline A M / Holderfield, Matthew / Wildes, David / Lowe, Scott W / Badgley, Michael A / Aguirre, Andrew J / Vonderheide, Robert H / Stanger, Ben Z / Baslan, Timour / Der, Channing J / Singh, Mallika / Olive, Kenneth P

    Nature

    2024  

    Abstract: Broad-spectrum RAS inhibition holds the potential to benefit roughly a quarter of human cancer patients whose tumors are driven by RAS ... ...

    Abstract Broad-spectrum RAS inhibition holds the potential to benefit roughly a quarter of human cancer patients whose tumors are driven by RAS mutations
    Language English
    Publishing date 2024-04-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/s41586-024-07379-z
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  3. Article ; Online: A range‐wide postglacial history of Swiss stone pine based on molecular markers and palaeoecological evidence

    Gugerli, Felix / Brodbeck, Sabine / Lendvay, Bertalan / Dauphin, Benjamin / Bagnoli, Francesca / van der Knaap, Willem O. / Tinner, Willy / Höhn, Mária / Vendramin, Giovanni G. / Morales‐Molino, César / Schwörer, Christoph

    Journal of Biogeography. 2023 June, v. 50, no. 6 p.1049-1062

    2023  

    Abstract: AIM: Knowing a species' response to historical climate shifts helps understanding its perspectives under global warming. We infer the hitherto unresolved postglacial history of Pinus cembra. Using independent evidence from genetic structure and ... ...

    Abstract AIM: Knowing a species' response to historical climate shifts helps understanding its perspectives under global warming. We infer the hitherto unresolved postglacial history of Pinus cembra. Using independent evidence from genetic structure and demographic inference of extant populations, and from palaeoecological findings, we derive putative refugia and re‐colonisation routes. LOCATION: European Alps and Carpathians. TAXA: Pinus cembra. METHODS: We genotyped nuclear and chloroplast microsatellite markers in nearly 3000 individuals from 147 locations across the entire natural range of P. cembra. Spatial genetic structure (Bayesian modelling) and demographic history (approximate Bayesian computation) were combined with palaeobotanical records (pollen, macrofossils) to infer putative refugial areas during the Last Glacial Maximum (LGM) and re‐colonisation of the current range. RESULTS: We found distinct spatial genetic structure, despite low genetic differentiation even between the two disjunct mountain ranges. Nuclear markers revealed five genetic clusters aligned East–West across the range, while chloroplast haplotype distribution suggested nine clusters. Spatially congruent separation at both marker types highlighted two main genetic lineages in the East and West of the range. Demographic inference supported early separation of these lineages dating back to a previous interstadial or interglacial c. 210,000 years ago. Differentiation into five biologically meaningful genetic clusters likely established during postglacial re‐colonisation. MAIN CONCLUSIONS: Combining genetic and palaeoecological evidence suggests that P. cembra primarily survived the LGM in ‘cold period’ refugia south of the Central European Alps and near the Carpathians, from where it expanded during the Late Glacial into its current Holocene ‘warm period’ refugia. This colonisation history has led to the distinct East–West structure of five genetic clusters. The two main genetic lineages likely derived from ancient divergence during an interglacial or interstadial. The respective contact zone (Brenner line) matches a main biogeographical break in the European Alps also found in herbaceous alpine plant species.
    Keywords Bayesian theory ; Holocene epoch ; Pinus cembra ; alpine plants ; biogeography ; chloroplasts ; climate ; genetic structure ; genetic variation ; geographical distribution ; haplotypes ; microsatellite repeats ; paleoecology ; pollen ; refuge habitats
    Language English
    Dates of publication 2023-06
    Size p. 1049-1062.
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 188963-1
    ISSN 0305-0270
    ISSN 0305-0270
    DOI 10.1111/jbi.14586
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Effects of adsorbed heat labile serum proteins and fibrinogen on adhesion and apoptosis of monocytes/macrophages on biomaterials.

    Brodbeck, William G / Colton, Erica / Anderson, James M

    Journal of materials science. Materials in medicine

    2003  Volume 14, Issue 8, Page(s) 671–675

    Abstract: A previously established human monocyte culture protocol was used to determine the effects of varying adsorbed proteins on monocyte/macrophage adhesion and survival on dimethyl-silane (DM) or RGD modified glass coverslips. Cells were allowed to adhere ... ...

    Abstract A previously established human monocyte culture protocol was used to determine the effects of varying adsorbed proteins on monocyte/macrophage adhesion and survival on dimethyl-silane (DM) or RGD modified glass coverslips. Cells were allowed to adhere for 2 h in the absence of protein or in the presence of serum, fibrinogen (Fg), heat inactivated serum (HIS), serum supplemented with Fg or HIS with Fg. Cell adhesion and apoptosis rates were determined on days 0 (2 h), 3, 7 and 10 of culture. The presence of serum alone in the initial culture was sufficient to optimize monocyte/macrophage adhesion and survival rates. Adding Fg to serum did not increase adhesion nor decrease apoptotic rates. No protein or the addition of HIS during the initial incubation period significantly decreased monocyte/macrophage adhesion and survival on both surfaces, however, the addition of Fg to HIS restored adhesion and survival rates to those seen with in the presence of serum alone on RGD surfaces. These studies demonstrate that monocyte/macrophage adhesion and survival on biomaterial surfaces are optimized by adsorbed heat labile serum proteins while adsorbed Fg plays a surface property-dependent role.
    Language English
    Publishing date 2003-09-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1031752-1
    ISSN 1573-4838 ; 0957-4530
    ISSN (online) 1573-4838
    ISSN 0957-4530
    DOI 10.1023/a:1024951330265
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  5. Article: Activation of caspase 3 during shear stress-induced neutrophil apoptosis on biomaterials.

    Shive, Matthew S / Brodbeck, William G / Anderson, James M

    Journal of biomedical materials research

    2002  Volume 62, Issue 2, Page(s) 163–168

    Abstract: Within the complex environment of an implanted cardiovascular device comprised of dynamic flow and foreign materials, phagocytic neutrophils may be ineffective in combating infection due to cellular responses to shear stress. This may be explained, in ... ...

    Abstract Within the complex environment of an implanted cardiovascular device comprised of dynamic flow and foreign materials, phagocytic neutrophils may be ineffective in combating infection due to cellular responses to shear stress. This may be explained, in part, by our recent reports of apoptosis of biomaterial-adherent leukocytes induced through exposure to shear stress. Here we utilize a rotating disk system to generate physiologically relevant shear stress levels (0-18 dynes/cm(2)) at the surface of a polyetherurethane urea (PEUU) and investigate neutrophil intracellular pathways involved in shear-induced apoptosis. In situ detection of activated caspases, the enzymatic mediators of the apoptosis cascade, showed qualitatively that these proteases participate in shear-induced apoptosis and are activated in a shear-dependent manner. The involvement of caspase 3 was confirmed through immunoprecipitation and immunoblotting of extracted neutrophil proteins. Comparative studies with neutrophils adherent under static conditions demonstrated time-dependent activation of caspases in TNF-alpha/cycloheximide-induced apoptosis, for which caspase-3 also was implicated. These findings are the first steps toward elucidation of the mechanisms behind the inappropriate induction of apoptosis by adhesion to biomaterials, which may contribute to the development and persistence of device-related infections.
    MeSH term(s) Apoptosis/drug effects ; Apoptosis/physiology ; Biocompatible Materials ; Caspase 3 ; Caspases/metabolism ; Cell Adhesion ; Enzyme Activation/physiology ; Humans ; Immunoblotting ; In Situ Hybridization ; In Vitro Techniques ; Neutrophils/drug effects ; Neutrophils/physiology ; Precipitin Tests ; Stress, Mechanical ; Tumor Necrosis Factor-alpha/pharmacology
    Chemical Substances Biocompatible Materials ; Tumor Necrosis Factor-alpha ; CASP3 protein, human (EC 3.4.22.-) ; Caspase 3 (EC 3.4.22.-) ; Caspases (EC 3.4.22.-)
    Language English
    Publishing date 2002-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 280321-5
    ISSN 1097-4636 ; 0021-9304
    ISSN (online) 1097-4636
    ISSN 0021-9304
    DOI 10.1002/jbm.10225
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    Zs.A 586: Show issues Location:
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  6. Article: Shear stress and material surface effects on adherent human monocyte apoptosis.

    Shive, Matthew S / Brodbeck, William G / Colton, Erica / Anderson, James M

    Journal of biomedical materials research

    2002  Volume 60, Issue 1, Page(s) 148–158

    Abstract: Monocytes play a critical role as both phagocytes and mediators of inflammatory responses in the prevention of cardiovascular device-related infections. However, persistent infection of these devices still occurs and may be attributed to deleterious ... ...

    Abstract Monocytes play a critical role as both phagocytes and mediators of inflammatory responses in the prevention of cardiovascular device-related infections. However, persistent infection of these devices still occurs and may be attributed to deleterious cellular alterations resulting from monocyte interactions with a foreign material in an environment of dynamic flow. Thus, the effects of both shear stress and adhesion to material surfaces on human monocyte apoptosis were investigated. A rotating disk system generated physiologically relevant shear stress levels (0-14 dyn/cm(2)), and shear-related apoptosis occurring in adherent monocytes was characterized. Using annexin V analysis, apoptosis of polyurethane-adherent monocytes under shear for 4 h increased to levels >70% with increasing shear in a near-linear fashion (r2 = 0.713). It was qualitatively confirmed using confocal microscopy that filamentous (F)-actin distribution was altered, that DNA fragmentation occurred, and that activated caspases were involved in shear-induced apoptosis. Static studies determined that spontaneous apoptosis was material-dependent over 72 h by demonstrating marked differences between apoptosis of monocytes adherent to a polyurethane compared to an alkyl-modified glass. Treatment with TNF-alpha augmented this material dependency in a dose-dependent fashion over time. F-actin content of TNF-alpha-treated cells decreased to <62% of untreated cells. We conclude that concomitant effects from both material surfaces and dynamic flow mediate human monocyte apoptosis and may have serious implications in the context of implanted cardiovascular device infection.
    MeSH term(s) Actins/chemistry ; Annexin A5 ; Apoptosis/physiology ; Biocompatible Materials ; Caspase Inhibitors ; Caspases/metabolism ; Cell Adhesion ; Enzyme Inhibitors/pharmacology ; Humans ; In Situ Nick-End Labeling ; In Vitro Techniques ; Microscopy, Electron, Scanning ; Monocytes/physiology ; Polyurethanes ; Rheology ; Signal Transduction/drug effects ; Stress, Mechanical ; Surface Properties ; Tumor Necrosis Factor-alpha/pharmacology
    Chemical Substances Actins ; Annexin A5 ; Biocompatible Materials ; Caspase Inhibitors ; Enzyme Inhibitors ; Polyurethanes ; Tumor Necrosis Factor-alpha ; polyetherurethane ; Caspases (EC 3.4.22.-)
    Language English
    Publishing date 2002-04
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 280321-5
    ISSN 1097-4636 ; 0021-9304
    ISSN (online) 1097-4636
    ISSN 0021-9304
    DOI 10.1002/jbm.10035
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  7. Article ; Online: Interrogation of transcriptomic changes associated with drug-induced hepatic sinusoidal dilatation in colorectal cancer.

    Jarzabek, Monika A / Proctor, William R / Vogt, Jennifer / Desai, Rupal / Dicker, Patrick / Cain, Gary / Raja, Rajiv / Brodbeck, Jens / Stevens, Dale / van der Stok, Eric P / Martens, John W M / Verhoef, Cornelis / Hegde, Priti S / Byrne, Annette T / Tarrant, Jacqueline M

    PloS one

    2018  Volume 13, Issue 6, Page(s) e0198099

    Abstract: Drug-related sinusoidal dilatation (SD) is a common form of hepatotoxicity associated with oxaliplatin-based chemotherapy used prior to resection of colorectal liver metastases (CRLM). Recently, hepatic SD has also been associated with anti-delta like 4 ( ...

    Abstract Drug-related sinusoidal dilatation (SD) is a common form of hepatotoxicity associated with oxaliplatin-based chemotherapy used prior to resection of colorectal liver metastases (CRLM). Recently, hepatic SD has also been associated with anti-delta like 4 (DLL4) cancer therapies targeting the NOTCH pathway. To investigate the hypothesis that NOTCH signaling plays an important role in drug-induced SD, gene expression changes were examined in livers from anti-DLL4 and oxaliplatin-induced SD in non-human primate (NHP) and patients, respectively. Putative mechanistic biomarkers of bevacizumab (bev)-mediated protection against oxaliplatin-induced SD were also investigated. RNA was extracted from whole liver sections or centrilobular regions by laser-capture microdissection (LCM) obtained from NHP administered anti-DLL4 fragment antigen-binding (F(ab')2 or patients with CRLM receiving oxaliplatin-based chemotherapy with or without bev. mRNA expression was quantified using high-throughput real-time quantitative PCR. Significance analysis was used to identify genes with differential expression patterns (false discovery rate (FDR) < 0.05). Eleven (CCL2, CCND1, EFNB2, ERG, ICAM1, IL16, LFNG, NOTCH1, NOTCH4, PRDX1, and TGFB1) and six (CDH5, EFNB2, HES1, IL16, MIK67, HES1 and VWF) candidate genes were differentially expressed in the liver of anti-DLL4- and oxaliplatin-induced SD, respectively. Addition of bev to oxaliplatin-based chemotherapy resulted in differential changes in hepatic CDH5, HEY1, IL16, JAG1, MMP9, NOTCH4 and TIMP1 expression. This work implicates NOTCH and IL16 pathways in the pathogenesis of drug-induced SD and further explains the hepato-protective effect of bev in oxaliplatin-induced SD observed in CRLM patients.
    MeSH term(s) Aged ; Animals ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Biopsy ; Capillaries/drug effects ; Capillaries/metabolism ; Capillaries/pathology ; Chemical and Drug Induced Liver Injury/genetics ; Colorectal Neoplasms/drug therapy ; Colorectal Neoplasms/pathology ; Dilatation, Pathologic/chemically induced ; Dilatation, Pathologic/genetics ; Female ; Gene Expression Profiling ; Humans ; Liver/blood supply ; Liver/drug effects ; Liver/metabolism ; Liver/pathology ; Liver Neoplasms/secondary ; Macaca fascicularis ; Male ; Middle Aged ; Neovascularization, Pathologic/chemically induced ; Neovascularization, Pathologic/genetics ; Oxaliplatin/administration & dosage ; Oxaliplatin/adverse effects ; Transcriptome/drug effects
    Chemical Substances Oxaliplatin (04ZR38536J)
    Language English
    Publishing date 2018-06-07
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0198099
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  8. Article: Lymphocytes and the foreign body response: lymphocyte enhancement of macrophage adhesion and fusion.

    Brodbeck, William G / Macewan, Matthew / Colton, Erica / Meyerson, Howard / Anderson, James M

    Journal of biomedical materials research. Part A

    2005  Volume 74, Issue 2, Page(s) 222–229

    Abstract: The host foreign body response ensues immediately following implantation of medical devices and prostheses. We have previously identified the role of macrophages in adhering to biomaterial surfaces and guiding the foreign body response while fusing into ... ...

    Abstract The host foreign body response ensues immediately following implantation of medical devices and prostheses. We have previously identified the role of macrophages in adhering to biomaterial surfaces and guiding the foreign body response while fusing into foreign body giant cells (FBGCs) and concentrating degradative and phagocytic activities. Despite their early and transient presence around implanted biomaterials, few studies have focused on the role of lymphocytes in the foreign body response and biocompatibility. To address this, an in vitro human lymphocyte/macrophage coculture system has been developed. Using this system, it has been shown that when lymphocytes are present during the initial adhesion of monocytes, the rate of monocyte adhesion and fusion is significantly increased (1,500 cells/mm2 and 60%, respectively) when compared to either no lymphocytes present (500 cells/mm2 adhesion and 0% fusion). Although lymphocytes adhered to the tissue culture polystyrene surface, 90% of the lymphocytes were associated with adherent macrophages. However, these cell-cell direct interactions were not necessary to influence macrophage adhesion or fusion as separating the two cell types by a Transwell insert still resulted in significantly increased levels of macrophage adhesion (p < 0.05 when compared to macrophage only cultures). Conversely, the presence of macrophages in Transwell experiments increased lymphocyte proliferation rates at all time points tested. These studies begin to detail the interactions between lymphocytes and macrophages in the absence of known antigen that appropriately relates to the scenarios experienced upon implantation of biomedical devices and the initiation of the foreign body response.
    MeSH term(s) Adult ; Cell Adhesion ; Cell Fusion ; Coculture Techniques ; Enzyme-Linked Immunosorbent Assay ; Foreign-Body Reaction/immunology ; Humans ; Lymphocytes/cytology ; Lymphocytes/immunology ; Lymphocytes/ultrastructure ; Macrophages/cytology ; Macrophages/immunology ; Macrophages/ultrastructure ; Microscopy, Electron, Scanning
    Language English
    Publishing date 2005-08-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 2099989-6
    ISSN 1552-4965 ; 1549-3296 ; 0021-9304
    ISSN (online) 1552-4965
    ISSN 1549-3296 ; 0021-9304
    DOI 10.1002/jbm.a.30313
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  9. Article: Student Research Award in the Undergraduate Degree Candidate category, 30th Annual Meeting of the Society for Biomaterials, Memphis, Tennessee, April 27-30, 2005. Monocyte/lymphocyte interactions and the foreign body response: in vitro effects of biomaterial surface chemistry.

    MacEwan, Matthew R / Brodbeck, William G / Matsuda, Takehisa / Anderson, James M

    Journal of biomedical materials research. Part A

    2005  Volume 74, Issue 3, Page(s) 285–293

    Abstract: To determine the effect of biomaterial surface chemistry on leukocyte interaction and activity at the material/tissue interface, human peripheral blood monocytes and lymphocytes were cultured on a series of poly(ethylene terephthalate) (PET)-based ... ...

    Abstract To determine the effect of biomaterial surface chemistry on leukocyte interaction and activity at the material/tissue interface, human peripheral blood monocytes and lymphocytes were cultured on a series of poly(ethylene terephthalate) (PET)-based biomaterials. Both monocytes and lymphocytes were isolated from whole human blood and separated by a nonadherent density centrifugation method before being plated on PET disks, surface modified by photograft copolymerization to yield hydrophobic, hydrophilic, anionic, and cationic surface properties. Monocytes and lymphocytes were cultured separately, to elicit baseline levels of activity, in direct coculture, to promote direct cell surface interactions, or in an indirect coculture system with both cell types separated by a -0.02-microm Transwell apparatus, to promote indirect paracrine interactions. Monocyte adhesion, macrophage fusion, and lymphocyte proliferation were measured on days 3, 7, 10, and 14 of culture. Results demonstrated that the presence of monocytes increased the activity of cocultured lymphocytes at the biomaterial/tissue interface, while the corresponding presence of lymphocytes increased the activation and fusion of indirectly cocultured monocytes. Biomaterial surface chemistry was also found to have a significant effect on monocyte adhesion and activation, and lymphocyte activity. Hydrophilic surfaces significantly inhibited both initial and longterm monocyte adhesion, and inhibited lymphocyte proliferation at longer time points. Anionic and cationic surfaces both exhibited mild inhibition of monocyte adhesion at prolonged time points and increased levels of macrophage fusion, while cationic surfaces decreased levels of lymphocyte proliferation and inhibited monocyte activity. These results elucidate the complex role of juxtacrine and paracrine interactions between monocytes and lymphocytes in the foreign body response, as well as promote the consideration of hydrophilic surfaces in future designs of implantable biomedical devices and prostheses.
    MeSH term(s) Biocompatible Materials/chemistry ; Cell Adhesion/physiology ; Cell Communication/physiology ; Cell Proliferation ; Cells, Cultured ; Coculture Techniques ; Foreign-Body Reaction/metabolism ; Foreign-Body Reaction/pathology ; Humans ; Lymphocytes/cytology ; Lymphocytes/metabolism ; Macrophages/metabolism ; Monocytes/metabolism ; Paracrine Communication/physiology ; Surface Properties
    Chemical Substances Biocompatible Materials
    Language English
    Publishing date 2005-03-31
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 2099989-6
    ISSN 1552-4965 ; 1549-3296 ; 0021-9304
    ISSN (online) 1552-4965
    ISSN 1549-3296 ; 0021-9304
    DOI 10.1002/jbm.a.30316
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  10. Article: Inhibition of macrophage development and foreign body giant cell formation by hydrophilic interpenetrating polymer network.

    Collier, Terry O / Anderson, James M / Brodbeck, William G / Barber, Thomas / Healy, Kevin E

    Journal of biomedical materials research. Part A

    2004  Volume 69, Issue 4, Page(s) 644–650

    Abstract: The ability of monocytes to adhere, differentiate into macrophages, and fuse to form foreign body giant cells (FBGCs) on an implanted material surface is a critical step toward biomaterial degradation. Novel homogeneous surfaces were utilized to mediate ... ...

    Abstract The ability of monocytes to adhere, differentiate into macrophages, and fuse to form foreign body giant cells (FBGCs) on an implanted material surface is a critical step toward biomaterial degradation. Novel homogeneous surfaces were utilized to mediate adhesion. These surfaces consisted of N-(2 aminoethyl)-3-aminopropyltrimethoxysilane (EDS) and an interpenetrating polymer network (IPN) of polyacrylamide and poly(ethylene glycol). These surfaces were designed to control cell adhesion and morphology and mediate cell differentiation, activation, metabolic ability, and apoptosis, resulting in a reduced or controlled inflammatory response. The EDS surface promotes cell adhesion and the IPN minimizes protein adsorption and subsequent cell adhesion. Both surfaces had similar cellular adhesion rates at each respective time point. However, the adherent macrophage morphology was similar at 2 h and day 3, and at days 7 and 10 adherent macrophages on the EDS surface formed FBGCs (46% at day 7 and 40% at day 10). Adherent cells on the IPN surface did not form FBGCs but instead formed monocyte aggregates (73% of adherent cells formed aggregates at day 7 and 63% at day 10). It is indicated that the two surface chemistries differentially controlled monocyte differentiation into macrophages and subsequent macrophage fusion to form FBGCs.
    MeSH term(s) Acrylic Resins/pharmacology ; Cell Adhesion/drug effects ; Cell Differentiation/drug effects ; Giant Cells, Foreign-Body/drug effects ; Humans ; Macrophages/cytology ; Macrophages/drug effects ; Monocytes/drug effects ; Polyethylene Glycols/pharmacology ; Polymers/pharmacology ; Silicone Elastomers/pharmacology
    Chemical Substances Acrylic Resins ; Polymers ; Silicone Elastomers ; aminoethyl-aminopropyl-trimethoxysilane (28ZCS5GA8G) ; Polyethylene Glycols (3WJQ0SDW1A) ; polyacrylamide (9003-05-8)
    Language English
    Publishing date 2004-05-24
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2099989-6
    ISSN 1552-4965 ; 1549-3296 ; 0021-9304
    ISSN (online) 1552-4965
    ISSN 1549-3296 ; 0021-9304
    DOI 10.1002/jbm.a.30030
    Database MEDical Literature Analysis and Retrieval System OnLINE

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