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  1. Article ; Online: Circulating platelets as liquid biopsy sources for cancer detection.

    Antunes-Ferreira, Mafalda / Koppers-Lalic, Danijela / Würdinger, Thomas

    Molecular oncology

    2020  Volume 15, Issue 6, Page(s) 1727–1743

    Abstract: Nucleic acids and proteins are shed into the bloodstream by tumor cells and can be exploited as biomarkers for the detection of cancer. In addition, cancer detection biomarkers can also be nontumor-derived, having their origin in other organs and cell ... ...

    Abstract Nucleic acids and proteins are shed into the bloodstream by tumor cells and can be exploited as biomarkers for the detection of cancer. In addition, cancer detection biomarkers can also be nontumor-derived, having their origin in other organs and cell types. Hence, liquid biopsies provide a source of direct tumor cell-derived biomolecules and indirect nontumor-derived surrogate markers that circulate in body fluids or are taken up by circulating peripheral blood cells. The capacity of platelets to take up proteins and nucleic acids and alter their megakaryocyte-derived transcripts and proteins in response to external signals makes them one of the richest liquid biopsy biosources. Platelets are the second most abundant cell type in peripheral blood and are routinely isolated through well-established and fast methods in clinical diagnostics but their value as a source of cancer biomarkers is relatively recent. Platelets do not have a nucleus but have a functional spliceosome and protein translation machinery, to process RNA transcripts. Platelets emerge as important repositories of potential cancer biomarkers, including several types of RNAs (mRNA, miRNA, circRNA, lncRNA, and mitochondrial RNA) and proteins, and several preclinical studies have highlighted their potential as a liquid biopsy source for detecting various types and stages of cancer. Here, we address the usability of platelets as a liquid biopsy for the detection of cancer. We describe several studies that support the use of platelet biomarkers in cancer diagnostics and discuss what is still lacking for their implementation into the clinic.
    MeSH term(s) Biomarkers, Tumor/blood ; Blood Platelets/pathology ; High-Throughput Nucleotide Sequencing ; Humans ; Liquid Biopsy ; Neoplasms/blood ; Neoplasms/diagnosis ; Neoplasms/genetics ; Neoplasms/pathology ; Translational Research, Biomedical
    Chemical Substances Biomarkers, Tumor
    Language English
    Publishing date 2020-12-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2415106-3
    ISSN 1878-0261 ; 1574-7891
    ISSN (online) 1878-0261
    ISSN 1574-7891
    DOI 10.1002/1878-0261.12859
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6637: Show issues Location:
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  2. Article ; Online: Tumor-educated platelets for the earlier detection of hepatocellular carcinoma.

    Best, Myron G / Wurdinger, Thomas

    Clinics and research in hepatology and gastroenterology

    2020  Volume 44, Issue 6, Page(s) 794–795

    MeSH term(s) Blood Platelets ; Carcinoma, Hepatocellular/diagnosis ; Humans ; Liquid Biopsy ; Liver Neoplasms/diagnosis
    Language English
    Publishing date 2020-04-22
    Publishing country France
    Document type Editorial ; Comment
    ZDB-ID 2594333-9
    ISSN 2210-741X ; 2210-7401
    ISSN (online) 2210-741X
    ISSN 2210-7401
    DOI 10.1016/j.clinre.2020.03.028
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  3. Book ; Online ; Thesis: Die Zwangsvollstreckung in Kryptowerte am Beispiel des Bitcoins

    Gassner, Daniel [Verfasser] / Würdinger, Markus [Akademischer Betreuer] / Riehm, Thomas [Akademischer Betreuer]

    2022  

    Author's details Daniel Gassner ; Markus Würdinger, Thomas Riehm
    Keywords Recht ; Law
    Subject code sg340
    Language German
    Publisher Universität Passau
    Publishing place Passau
    Document type Book ; Online ; Thesis
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  4. Article ; Online: Blood platelets as an RNA biomarker platform for neuro-oncological diseases.

    Post, Edward / Sol, Nik / Best, Myron G / Wurdinger, Thomas

    Neuro-oncology advances

    2022  Volume 4, Issue Suppl 2, Page(s) ii61–ii65

    Abstract: Blood-based liquid biopsies are an upcoming approach for earlier cancer detection, diagnostics, prognostics, therapy-response prediction, and therapy monitoring, including in patients with tumors of the central nervous system. Among these, liquid ... ...

    Abstract Blood-based liquid biopsies are an upcoming approach for earlier cancer detection, diagnostics, prognostics, therapy-response prediction, and therapy monitoring, including in patients with tumors of the central nervous system. Among these, liquid biopsies are plasma-derived markers such as cell-free DNA, RNA and proteins, extracellular vesicles, circulating glioma cells, immune cells, and blood platelets. Blood platelets are involved in the local and systemic response to the presence of cancer, thereby sequestering and splicing RNAs, which may be clinically useful as blood-based biomarkers. In this review, we discuss the available literature regarding the role of blood platelets in gliomas and provide suggestions for future research efforts.
    Language English
    Publishing date 2022-11-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 3009682-0
    ISSN 2632-2498 ; 2632-2498
    ISSN (online) 2632-2498
    ISSN 2632-2498
    DOI 10.1093/noajnl/vdac043
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  5. Article ; Online: Platelets and tumor-associated RNA transfer.

    D'Ambrosi, Silvia / Nilsson, R Jonas / Wurdinger, Thomas

    Blood

    2021  Volume 137, Issue 23, Page(s) 3181–3191

    Abstract: Until recently, the nucleic acid content of platelets was considered to be fully determined by their progenitor megakaryocyte. However, it is now well understood that additional mediators (eg, cancer cells) can intervene, thereby influencing the RNA ... ...

    Abstract Until recently, the nucleic acid content of platelets was considered to be fully determined by their progenitor megakaryocyte. However, it is now well understood that additional mediators (eg, cancer cells) can intervene, thereby influencing the RNA repertoire of platelets. Platelets are highly dynamic cells that are able to communicate and influence their environment. For instance, platelets have been involved in various steps of cancer development and progression by supporting tumor growth, survival, and dissemination. Cancer cells can directly and/or indirectly influence platelet RNA content, resulting in tumor-mediated "education" of platelets. Alterations in the tumor-educated platelet RNA profile have been described as a novel source of potential biomarkers. Individual platelet RNA biomarkers as well as complex RNA signatures may be used for early detection of cancer and treatment monitoring. Here, we review the RNA transfer occurring between cancer cells and platelets. We explore the potential use of platelet RNA biomarkers as a liquid biopsy biosource and discuss methods to evaluate the transcriptomic content of platelets.
    MeSH term(s) Biomarkers, Tumor/metabolism ; Blood Platelets/metabolism ; Blood Platelets/pathology ; Cell Communication ; Humans ; Neoplasms/diagnosis ; Neoplasms/metabolism ; Neoplasms/pathology ; RNA, Neoplasm/metabolism ; RNA, Transfer/metabolism
    Chemical Substances Biomarkers, Tumor ; RNA, Neoplasm ; RNA, Transfer (9014-25-9)
    Language English
    Publishing date 2021-05-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 80069-7
    ISSN 1528-0020 ; 0006-4971
    ISSN (online) 1528-0020
    ISSN 0006-4971
    DOI 10.1182/blood.2019003978
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uh III Zs.140: Show issues Location:
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  6. Article: miRGalaxy: Galaxy-Based Framework for Interactive Analysis of microRNA and isomiR Sequencing Data.

    Glogovitis, Ilias / Yahubyan, Galina / Würdinger, Thomas / Koppers-Lalic, Danijela / Baev, Vesselin

    Cancers

    2021  Volume 13, Issue 22

    Abstract: Tools for microRNA (miR) sequencing data analyses are broadly used in biomedical research. However, the complexity of computational approaches still remains a challenge for biologists with scarce experience in data analytics and bioinformatics. Here, we ... ...

    Abstract Tools for microRNA (miR) sequencing data analyses are broadly used in biomedical research. However, the complexity of computational approaches still remains a challenge for biologists with scarce experience in data analytics and bioinformatics. Here, we present miRGalaxy, a Galaxy-based framework for comprehensive analysis of miRs and their sequence variants-miR isoforms (isomiRs). Though isomiRs are commonly reported in deep-sequencing experiments, their detailed structure complexity and specific differential expression (DE) remain not fully examined by the majority of the available analysis tools. miRGalaxy encompasses biologist-user-friendly tools and workflows dedicated to the analysis of the isomiR-ome and its complex behavior in various biological samples. miRGalaxy is developed as a modular, accessible, redistributable, shareable, and user-friendly framework for scientists working with small RNA (sRNA)-seq data. Due to its modular workflow, advanced users can customize the steps and tools for their needs. In addition, the framework provides an analysis report where the significant output results are summarized in charts and visualizations. miRGalaxy can be accessed via preconfigured Docker image flavor and a Toolshed installation if the user already has a running Galaxy instance. Over the last decade, studies on the expression of miRs and isomiRs in normal and deregulated tissues have led to the discovery of their potential as diagnostic biomarkers. The detection of miRs in biofluids further expanded the exploration of the miR repertoire as a source of liquid biopsy biomarkers. Here we show the miRGalaxy framework application for in-depth analysis of the sRNA-seq data from two different biofluids, milk and plasma, to identify, annotate, and discover specific differentially expressed miRs and isomiRs.
    Language English
    Publishing date 2021-11-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers13225663
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  7. Article ; Online: GBM Drug Bank-a new resource for glioblastoma drug discovery and informatics research.

    Svensson, Fredrik / Westerman, Bart / Würdinger, Thomas / Bailey, David

    Neuro-oncology

    2018  Volume 20, Issue 12, Page(s) 1680–1681

    MeSH term(s) Antineoplastic Agents/pharmacology ; Biomedical Research ; Brain Neoplasms/drug therapy ; Databases, Pharmaceutical ; Drug Discovery ; Glioblastoma/drug therapy ; Humans ; Medical Informatics
    Chemical Substances Antineoplastic Agents
    Language English
    Publishing date 2018-08-23
    Publishing country England
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 2028601-6
    ISSN 1523-5866 ; 1522-8517
    ISSN (online) 1523-5866
    ISSN 1522-8517
    DOI 10.1093/neuonc/noy122
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5307: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  8. Article: Antikanker coronavirussen.

    Würdinger, Thomas

    Tijdschrift voor diergeneeskunde

    2007  Volume 132, Issue 3, Page(s) 90–92

    Title translation Anticancer coronaviruses.
    MeSH term(s) Animals ; Apoptosis ; Cell Division ; Coronavirus/immunology ; Coronavirus/physiology ; Humans ; Neoplasms/therapy ; Neoplasms/virology
    Language Dutch
    Publishing date 2007-02-01
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 422558-2
    ISSN 0040-7453
    ISSN 0040-7453
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1158: Show issues Location:
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  9. Article ; Online: The immunological landscape of peripheral blood in glioblastoma patients and immunological consequences of age and dexamethasone treatment.

    Dusoswa, Sophie A / Verhoeff, Jan / van Asten, Saskia / Lübbers, Joyce / van den Braber, Marlous / Peters, Sophie / Abeln, Sanne / Crommentuijn, Matheus H W / Wesseling, Pieter / Vandertop, William Peter / Twisk, Jos W R / Würdinger, Thomas / Noske, David / van Kooyk, Yvette / Garcia-Vallejo, Juan J

    Frontiers in immunology

    2024  Volume 15, Page(s) 1343484

    Abstract: Background: Glioblastomas manipulate the immune system both locally and systemically, yet, glioblastoma-associated changes in peripheral blood immune composition are poorly studied. Age and dexamethasone administration in glioblastoma patients have been ...

    Abstract Background: Glioblastomas manipulate the immune system both locally and systemically, yet, glioblastoma-associated changes in peripheral blood immune composition are poorly studied. Age and dexamethasone administration in glioblastoma patients have been hypothesized to limit the effectiveness of immunotherapy, but their effects remain unclear. We compared peripheral blood immune composition in patients with different types of brain tumor to determine the influence of age, dexamethasone treatment, and tumor volume.
    Methods: High-dimensional mass cytometry was used to characterise peripheral blood mononuclear cells of 169 patients with glioblastoma, lower grade astrocytoma, metastases and meningioma. We used blood from medically-refractory epilepsy patients and healthy controls as control groups. Immune phenotyping was performed using FlowSOM and t-SNE analysis in R followed by supervised annotation of the resulting clusters. We conducted multiple linear regression analysis between intracranial pathology and cell type abundance, corrected for clinical variables. We tested correlations between cell type abundance and survival with Cox-regression analyses.
    Results: Glioblastoma patients had significantly fewer naive CD4+ T cells, but higher percentages of mature NK cells than controls. Decreases of naive CD8+ T cells and alternative monocytes and an increase of memory B cells in glioblastoma patients were influenced by age and dexamethasone treatment, and only memory B cells by tumor volume. Progression free survival was associated with percentages of CD4+ regulatory T cells and double negative T cells.
    Conclusion: High-dimensional mass cytometry of peripheral blood in patients with different types of intracranial tumor provides insight into the relation between intracranial pathology and peripheral immune status. Wide immunosuppression associated with age and pre-operative dexamethasone treatment provide further evidence for their deleterious effects on treatment with immunotherapy.
    MeSH term(s) Humans ; Glioblastoma/drug therapy ; Glioblastoma/pathology ; Leukocytes, Mononuclear/pathology ; CD4-Positive T-Lymphocytes ; Immunotherapy/methods ; Dexamethasone/therapeutic use
    Chemical Substances Dexamethasone (7S5I7G3JQL)
    Language English
    Publishing date 2024-01-18
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2024.1343484
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  10. Article ; Online: A novel score to predict in-hospital mortality for patients with acute coronary syndrome and out-of-hospital cardiac arrest: the FACTOR study.

    Schweiger, Victor / Hiller, Pauline / Utters, Rahel / Fenice, Angela / Cammann, Victoria Lucia / Di Vece, Davide / Rajman, Katja / Candreva, Alessandro / Gotschy, Alexander / Gilhofer, Thomas / Würdinger, Michael / Stähli, Barbara E / Seifert, Burkhardt / Müller, Stefan M / Templin, Christian / Stehli, Julia

    Clinical research in cardiology : official journal of the German Cardiac Society

    2024  Volume 113, Issue 4, Page(s) 591–601

    Abstract: Introduction: Acute coronary syndromes (ACS) represent a substantial global healthcare challenge. In its most severe form, it can lead to out-of-hospital cardiac arrest (OHCA). Despite medical advancements, survival rates in OHCA patients remain low. ... ...

    Abstract Introduction: Acute coronary syndromes (ACS) represent a substantial global healthcare challenge. In its most severe form, it can lead to out-of-hospital cardiac arrest (OHCA). Despite medical advancements, survival rates in OHCA patients remain low. Further, the prediction of outcomes in these patients poses a challenge to all health care providers involved. This study aims at developing a score with variables available on admission to assess in-hospital mortality of patients with OHCA undergoing coronary angiography.
    Method: All patients with OHCA due to ACS admitted to a tertiary care center were included. A multivariate logistic regression analysis was conducted to explore the association between clinical variables and in-hospital all-cause mortality. A scoring system incorporating variables available upon admission to assess individual patients' risk of in-hospital mortality was developed (FACTOR score). The score was then validated.
    Results: A total of 291 patients were included in the study, with a median age of 65 [56-73] years, including 47 women (16.2%). The in-hospital mortality rate was 41.2%. A prognostic model was developed in the derivation cohort (n = 138) and included the following variables: age, downtime, first detected rhythm, and administration of epinephrine. The area under the curve for the FACTOR score was 0.823 (95% CI 0.737-0.894) in the derivation cohort and 0.828 (0.760-0.891) in the validation cohort (n = 153).
    Conclusion: The FACTOR score demonstrated a reliable prognostic tool for health care providers in assessing in-hospital mortality of OHCA patients. Early acknowledgement of a poor prognosis may help in patient management and allocation of resources.
    MeSH term(s) Humans ; Female ; Middle Aged ; Aged ; Out-of-Hospital Cardiac Arrest/diagnosis ; Out-of-Hospital Cardiac Arrest/therapy ; Acute Coronary Syndrome/diagnosis ; Acute Coronary Syndrome/therapy ; Hospital Mortality ; Prognosis ; Hospitalization ; Cardiopulmonary Resuscitation
    Language English
    Publishing date 2024-02-08
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2213295-8
    ISSN 1861-0692 ; 1861-0684
    ISSN (online) 1861-0692
    ISSN 1861-0684
    DOI 10.1007/s00392-023-02367-1
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