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Article: Volatiles from ripe fruits of karaka (Corynocarpus laevigatus J.R. et G. Forst.)

Fountain, D.W / Couchman, K

New Zealand journal of botany. 1984. v. 22 (3)

1984

Keywords	volatile compounds ; chemical constituents of plants
Language	English
Size	p. 341-343., ill.
Document type	Article
ZDB-ID	2098798-5
ISSN	1175-8643 ; 0028-825X
ISSN (online)	1175-8643
ISSN	0028-825X
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Post mortem blood bromazolam concentrations and co-findings in 96 coronial cases within England and Wales.

Hikin, L J / Coombes, G / Rice-Davies, K / Couchman, L / Smith, P R / Morley, S R

Forensic science international

2023 Volume 354, Page(s) 111891

Abstract: Bromazolam is a newly emerging benzodiazepine drug which is not licensed for medicinal use. It may be sourced as a New Psychoactive Substance (NPS) for its desired effects or be consumed unknowingly via counterfeit Xanax® or Valium® preparations. As part ...

Abstract	Bromazolam is a newly emerging benzodiazepine drug which is not licensed for medicinal use. It may be sourced as a New Psychoactive Substance (NPS) for its desired effects or be consumed unknowingly via counterfeit Xanax® or Valium® preparations. As part of our Coronial workload, we observed an increase in the detection of bromazolam from September 2021 to November 2022. We report a series of 96 cases in which bromazolam was quantitated by high resolution accurate mass - mass spectrometry (HRAM - MS) in post-mortem blood. The mean (SD) post-mortem blood bromazolam concentration from our case series was 64.6 ( ± 79.4) µg/L (range <1-425 µg/L). Routine toxicological screening results have also been reported; the most commonly encountered drugs taken in combination with bromazolam were cocaine, gabapentinoids and diazepam. In 48% of cases at least one further designer benzodiazepine drug was also present (etizolam, flualprazolam, flubromazolam, flubromazepam). It is essential that laboratories providing toxicological investigations are aware of the limitations of their assays; and inclusion of bromazolam within targeted screening panels using LC-MS/MS is encouraged. Bromazolam has not been associated with death in isolation from resulting toxic concentrations; however, it is likely to enhance adverse clinical effects when taken in combination with stimulant and/or centrally-acting depressant drugs (poly-drug deaths). Bromazolam, similar to other benzodiazepines, may also impair cognition and decision making skills.
MeSH term(s)	Designer Drugs/adverse effects ; Chromatography, Liquid ; Wales ; Tandem Mass Spectrometry ; Benzodiazepines ; England
Chemical Substances	Designer Drugs ; Benzodiazepines (12794-10-4)
Language	English
Publishing date	2023-11-28
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	424042-x
ISSN	1872-6283 ; 0379-0738
ISSN (online)	1872-6283
ISSN	0379-0738
DOI	10.1016/j.forsciint.2023.111891
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Article ; Online: Use of Nonhuman Sera as a Highly Cost-Effective Internal Standard for Quantitation of Multiple Human Proteins Using Species-Specific Tryptic Peptides: Applicability in Clinical LC-MS Analyses.

Williams, Geraldine / Couchman, Lewis / Taylor, David R / Sandhu, Jatinderpal K / Slingsby, Oliver C / Ng, Leong L / Moniz, Cajetan F / Jones, Donald J L / Maxwell, Colleen B

Journal of proteome research

2024

Abstract: Quantitation of proteins using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is complex, with a multiplicity of options ranging from label-free techniques to chemically and metabolically labeling proteins. Increasingly, for clinically ... ...

Abstract	Quantitation of proteins using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is complex, with a multiplicity of options ranging from label-free techniques to chemically and metabolically labeling proteins. Increasingly, for clinically relevant analyses, stable isotope-labeled (SIL) internal standards (ISs) represent the "gold standard" for quantitation due to their similar physiochemical properties to the analyte, wide availability, and ability to multiplex to several peptides. However, the purchase of SIL-ISs is a resource-intensive step in terms of cost and time, particularly for screening putative biomarker panels of hundreds of proteins. We demonstrate an alternative strategy utilizing nonhuman sera as the IS for quantitation of multiple human proteins. We demonstrate the effectiveness of this strategy using two high abundance clinically relevant analytes, vitamin D binding protein [Gc globulin] (DBP) and albumin (ALB). We extend this to three putative risk markers for cardiovascular disease: plasma protease C1 inhibitor (SERPING1), annexin A1 (ANXA1), and protein kinase, DNA-activated catalytic subunit (PRKDC). The results show highly specific, reproducible, and linear measurement of the proteins of interest with comparable precision and accuracy to the gold standard SIL-IS technique. This approach may not be applicable to every protein, but for many proteins it can offer a cost-effective solution to LC-MS/MS protein quantitation.
Language	English
Publishing date	2024-03-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.3c00762
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Article ; Online: Plasma and Urine Levamisole in Clinical Samples Containing Benzoylecgonine: Absence of Aminorex.

Handley, S A / Belsey, S L / Couchman, L / Flanagan, R J

Journal of analytical toxicology

2018 Volume 43, Issue 4, Page(s) 299–306

Abstract: Aminorex has been reported as a metabolite of levamisole in man, but data on the aminorex concentrations in clinical samples are scant. We thus measured levamisole, aminorex and benzoylecgonine in urine, and levamisole and aminorex in plasma using ... ...

Abstract	Aminorex has been reported as a metabolite of levamisole in man, but data on the aminorex concentrations in clinical samples are scant. We thus measured levamisole, aminorex and benzoylecgonine in urine, and levamisole and aminorex in plasma using achiral liquid chromatography-high resolution mass spectrometry. Centrifuged urine (50 μL) was diluted with LC eluent containing internal standard (benzoylecgonine-D3, 25 μg/L) (450 μL). For plasma, sample (200 μL) and Tris solution (2 mol/L, pH 10.6, 100 μL) were added to a 60.5 × 7.5 mm i.d. glass test tube. Internal standard solution (ketamine-D4, 200 μg/L) (10 μL) was added and the tube contents vortex-mixed (5 s). Butyl acetate:butanol (9 + 1, v/v; 200 μL) was added and after vortex-mixing (30 s) and centrifugation (13,680 × g, 4 min), the extract was evaporated to dryness and reconstituted in 10 mmol/L aqueous ammonium formate containing 0.1% (v/v) formic acid (150 μL). Prepared samples and extracts (100 μL) were analyzed using an AccucoreTM Phenyl-Hexyl column (2.6 mm a.p.s., 100 × 2.1 mm i.d.) maintained at 40°C. MS detection was in positive mode using heated electrospray ionization (ThermoFisher Q-ExactiveTM). Intra- and inter-assay accuracy and precision were ±20%, and ≤11%, respectively, for all analytes in both matrices. Lower limits of quantitation were 0.1 and 1 μg/L (all analytes) in plasma and urine, respectively. Of 100 consecutive urine samples submitted for drugs of abuse screening containing benzoylecgonine, levamisole was detected in 72 (median 565, range 4-72,970 μg/L). Levamisole was also measured in eight plasma samples (median 10.6, range 0.9-64.1 μg/L). A number of metabolites of levamisole (4-hydroxylevamisole, levamisole sulfoxide, levamisole glucuronide, and hydroxylevamisole glucuronide) were tentatively identified in urine. Neither aminorex, nor any of its reported metabolites were detected in any sample.
MeSH term(s)	Adult ; Aged ; Agranulocytosis/etiology ; Aminorex/blood ; Aminorex/urine ; Antinematodal Agents/adverse effects ; Antinematodal Agents/blood ; Antinematodal Agents/chemistry ; Antinematodal Agents/urine ; Appetite Depressants/blood ; Appetite Depressants/urine ; Chromatography, Liquid ; Cocaine/analogs & derivatives ; Cocaine/urine ; Drug Contamination ; Female ; Half-Life ; Humans ; Levamisole/adverse effects ; Levamisole/blood ; Levamisole/chemistry ; Levamisole/urine ; Male ; Middle Aged ; Osmolar Concentration ; Street Drugs ; Substance Abuse Detection/methods ; Tandem Mass Spectrometry ; Vasculitis/etiology ; Vasoconstrictor Agents/urine ; Young Adult
Chemical Substances	Antinematodal Agents ; Appetite Depressants ; Street Drugs ; Vasoconstrictor Agents ; Levamisole (2880D3468G) ; Aminorex (2SH16612I9) ; benzoylecgonine (5353I8I6YS) ; Cocaine (I5Y540LHVR)
Language	English
Publishing date	2018-12-26
Publishing country	England
Document type	Journal Article
ZDB-ID	752391-9
ISSN	1945-2403 ; 0146-4760
ISSN (online)	1945-2403
ISSN	0146-4760
DOI	10.1093/jat/bky102
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Zs.A 1333: Show issues

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Article ; Online: Pelvic Floor Dysfunction After Childbirth: Occupational Impact and Awareness of Available Treatment.

Burkhart, Rebecca / Couchman, Katlin / Crowell, Katherine / Jeffries, Samantha / Monvillers, Sarah / Vilensky, Joel

OTJR : occupation, participation and health

2020 Volume 41, Issue 2, Page(s) 108–115

Abstract: Postpartum pelvic floor dysfunction (PFD) has been identified as a significant public health issue. The study aimed to (a) identify the impact of PFD on parous women's occupations, (b) explore how PFD impacts daily life, and (c) determine the level of ... ...

Abstract	Postpartum pelvic floor dysfunction (PFD) has been identified as a significant public health issue. The study aimed to (a) identify the impact of PFD on parous women's occupations, (b) explore how PFD impacts daily life, and (c) determine the level of awareness regarding availability of PFD treatment. The preliminary study utilized a mixed-methods descriptive design. In total, 255 participants completed the Mothers and Children (MAC) survey, distributed electronically throughout the United States. Results indicated that PFD symptoms limit parous women's occupational performance, specifically sexual activity and exercise. Despite results indicating PFD significantly detracted from participants' daily life, 66.8% of participants reported having no awareness of pelvic floor rehabilitation to address PFD. Findings suggest that further research and increased advocacy within the field of occupational therapy are necessary to increase the awareness of PFD rehabilitation and mitigate the impact on women's lives after childbirth.
MeSH term(s)	Child ; Female ; Humans ; Pelvic Floor ; Pelvic Floor Disorders ; Pregnancy ; Surveys and Questionnaires
Language	English
Publishing date	2020-11-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	2081243-7
ISSN	1938-2383 ; 1539-4492 ; 0276-1599
ISSN (online)	1938-2383
ISSN	1539-4492 ; 0276-1599
DOI	10.1177/1539449220970881
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Article ; Online: Femoral blood concentrations of the designer benzodiazepine etizolam in post-mortem cases.

Hikin, Laura J / Smith, Paul R / Maskell, Peter D / Kurimbokus, Hassan / Ashong, Emily / Couchman, Lewis / Morley, Stephen R

Medicine, science, and the law

2020 Volume 61, Issue 2, Page(s) 122–129

Abstract: Etizolam is a thienodiazepine that although licensed for clinical usage in Japan, India and South Korea is commonly abused and detected in post-mortem cases around the world. To date, there are limited data in the literature to allow for the ... ...

Abstract	Etizolam is a thienodiazepine that although licensed for clinical usage in Japan, India and South Korea is commonly abused and detected in post-mortem cases around the world. To date, there are limited data in the literature to allow for the interpretation of blood concentrations of etizolam in post-mortem cases. A liquid chromatography with tandem mass spectrometry method was used to quantitate etizolam concentrations in 28 post-mortem cases where etizolam was detected. The median concentration of etizolam in femoral blood was 8.5 ng/mL (range 1.0-172.0 ng/mL;
MeSH term(s)	Adult ; Autopsy ; Blood Chemical Analysis ; Chromatography, High Pressure Liquid ; Diazepam/analogs & derivatives ; Diazepam/blood ; Female ; Femoral Vein ; Forensic Toxicology ; Humans ; Male ; Middle Aged ; Substance Abuse Detection/methods ; Tandem Mass Spectrometry ; United Kingdom
Chemical Substances	etizolam (A76XI0HL37) ; Diazepam (Q3JTX2Q7TU)
Language	English
Publishing date	2020-11-25
Publishing country	England
Document type	Journal Article
ZDB-ID	301137-9
ISSN	2042-1818 ; 0025-8024
ISSN (online)	2042-1818
ISSN	0025-8024
DOI	10.1177/0025802420973814
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Article ; Online: The EDGE2 protocol: Advancing the prioritisation of Evolutionarily Distinct and Globally Endangered species for practical conservation action.

Gumbs, Rikki / Gray, Claudia L / Böhm, Monika / Burfield, Ian J / Couchman, Olivia R / Faith, Daniel P / Forest, Félix / Hoffmann, Michael / Isaac, Nick J B / Jetz, Walter / Mace, Georgina M / Mooers, Arne O / Safi, Kamran / Scott, Oenone / Steel, Mike / Tucker, Caroline M / Pearse, William D / Owen, Nisha R / Rosindell, James

PLoS biology

2023 Volume 21, Issue 2, Page(s) e3001991

Abstract: The conservation of evolutionary history has been linked to increased benefits for humanity and can be captured by phylogenetic diversity (PD). The Evolutionarily Distinct and Globally Endangered (EDGE) metric has, since 2007, been used to prioritise ... ...

Abstract	The conservation of evolutionary history has been linked to increased benefits for humanity and can be captured by phylogenetic diversity (PD). The Evolutionarily Distinct and Globally Endangered (EDGE) metric has, since 2007, been used to prioritise threatened species for practical conservation that embody large amounts of evolutionary history. While there have been important research advances since 2007, they have not been adopted in practice because of a lack of consensus in the conservation community. Here, building from an interdisciplinary workshop to update the existing EDGE approach, we present an "EDGE2" protocol that draws on a decade of research and innovation to develop an improved, consistent methodology for prioritising species conservation efforts. Key advances include methods for dealing with uncertainty and accounting for the extinction risk of closely related species. We describe EDGE2 in terms of distinct components to facilitate future revisions to its constituent parts without needing to reconsider the whole. We illustrate EDGE2 by applying it to the world's mammals. As we approach a crossroads for global biodiversity policy, this Consensus View shows how collaboration between academic and applied conservation biologists can guide effective and practical priority-setting to conserve biodiversity.
MeSH term(s)	Animals ; Endangered Species ; Phylogeny ; Biodiversity ; Biological Evolution ; Humanities ; Mammals
Language	English
Publishing date	2023-02-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2126776-5
ISSN	1545-7885 ; 1544-9173
ISSN (online)	1545-7885
ISSN	1544-9173
DOI	10.1371/journal.pbio.3001991
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Article: Redefining the role of syndecans in C. elegans biology.

Gopal, Sandeep / Couchman, John / Pocock, Roger

Worm

2016 Volume 5, Issue 1, Page(s) e1142042

Abstract: Cytosolic calcium is an important factor during fertilization, development and differentiation. Hence, the control of cytosolic calcium levels has been studied extensively for several decades. Numerous calcium channels have been identified and their ... ...

Abstract	Cytosolic calcium is an important factor during fertilization, development and differentiation. Hence, the control of cytosolic calcium levels has been studied extensively for several decades. Numerous calcium channels have been identified and their mechanism of action elucidated. However, the mode of calcium channel regulation remains elusive. Here we discuss our recent findings regarding the role of syndecans in the regulation of cytosolic calcium levels. Syndecans are transmembrane proteoglycans present in both vertebrates and invertebrates that interact with extracellular ligands resulting in the activation of several downstream signaling pathways. We identified a previously unappreciated role of syndecans in cytosolic calcium regulation in mammals that is conserved in C. elegans. We concluded that calcium regulation is the basic, evolutionarily conserved role for syndecans, which enables them to be integral for multiple cellular functions.
Language	English
Publishing date	2016-01-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	2682460-7
ISSN	2162-4054 ; 2162-4046
ISSN (online)	2162-4054
ISSN	2162-4046
DOI	10.1080/21624054.2016.1142042
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Article ; Online: Wnt Signaling Cascades and the Roles of Syndecan Proteoglycans.

Pataki, Csilla A / Couchman, John R / Brábek, Jan

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

2015 Volume 63, Issue 7, Page(s) 465–480

Abstract: Wnt signaling comprises a group of pathways emanating from the extracellular environment through cell-surface receptors into the intracellular milieu. Wnt signaling cascades can be divided into two main branches, the canonical/β-catenin pathway and the ... ...

Abstract	Wnt signaling comprises a group of pathways emanating from the extracellular environment through cell-surface receptors into the intracellular milieu. Wnt signaling cascades can be divided into two main branches, the canonical/β-catenin pathway and the non-canonical pathways containing the Wnt/planar cell polarity and Wnt/calcium signaling. Syndecans are type I transmembrane proteoglycans with a long evolutionary history, being expressed in all Bilateria and in almost all cell types. Both Wnt pathways have been extensively studied over the past 30 years and shown to have roles during development and in a multitude of diseases. Although the first evidence for interactions between syndecans and Wnts dates back to 1997, the number of studies connecting these pathways is low, and many open questions remained unanswered. In this review, syndecan's involvement in Wnt signaling pathways as well as some of the pathologies resulting from dysregulation of the components of these pathways are summarized.
MeSH term(s)	Animals ; Disease ; Humans ; Syndecans/chemistry ; Syndecans/metabolism ; Wnt Signaling Pathway
Chemical Substances	Syndecans
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	218208-7
ISSN	1551-5044 ; 0022-1554
ISSN (online)	1551-5044
ISSN	0022-1554
DOI	10.1369/0022155415586961
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Article ; Online: Proteoglycan isolation and analysis.

Woods, A / Couchman, J R

Current protocols in cell biology

2008 Volume Chapter 10, Page(s) Unit 10.7

Abstract: Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked ... ...

Abstract	Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.
MeSH term(s)	Animals ; Cells, Cultured ; Extracellular Matrix/chemistry ; Glycosaminoglycans/chemistry ; Glycosaminoglycans/isolation & purification ; Humans ; Proteoglycans/chemistry ; Proteoglycans/isolation & purification
Chemical Substances	Glycosaminoglycans ; Proteoglycans
Language	English
Publishing date	2008-01-23
Publishing country	United States
Document type	Journal Article
ISSN	1934-2616
ISSN (online)	1934-2616
DOI	10.1002/0471143030.cb1007s07
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