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  1. Article ; Online: Domain-wise differentiation of Mycobacterium tuberculosis H

    Beg, Md Amjad / Hejazi, Iram Iqbal / Thakur, Sonu Chand / Athar, Fareeda

    Biotechnology and applied biochemistry

    2021  Volume 69, Issue 1, Page(s) 296–312

    Abstract: Proteomic information revealed approximately 3,923 proteins in Mycobacterium tuberculosis ... ...

    Abstract Proteomic information revealed approximately 3,923 proteins in Mycobacterium tuberculosis H
    MeSH term(s) Antitubercular Agents ; Bacterial Proteins/genetics ; Molecular Docking Simulation ; Mycobacterium tuberculosis/genetics ; Proteomics
    Chemical Substances Antitubercular Agents ; Bacterial Proteins
    Language English
    Publishing date 2021-02-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 883433-7
    ISSN 1470-8744 ; 0885-4513
    ISSN (online) 1470-8744
    ISSN 0885-4513
    DOI 10.1002/bab.2109
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1865: Show issues Location:
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  2. Article ; Online: Glossary of phytoconstituents: Can these be repurposed against SARS CoV-2? A quick in silico screening of various phytoconstituents from plant Glycyrrhiza glabra with SARS CoV-2 main protease.

    Hejazi, Iram Iqbal / Beg, Md Amjad / Imam, Md Ali / Athar, Fareeda / Islam, Asimul

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

    2021  Volume 150, Page(s) 112057

    Abstract: World is familiar with the viral pathogen Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2). The principle working enzymes of SARS CoV-2 have been identified as main proteases 3Cl pro which act as main regulators for SARS infection. The need ... ...

    Abstract World is familiar with the viral pathogen Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2). The principle working enzymes of SARS CoV-2 have been identified as main proteases 3Cl pro which act as main regulators for SARS infection. The need for therapy is required immediately pertaining to the vulnerable conditions. Protein-ligand studies are imperative for understanding the functioning of biological interactions as they are crucial in providing a hypothetical origin for the design and unearthing of novel drug targets. Phytoconstituents from Glycyrrhiza glabra, earlier reported to be anticancerous in nature were used as repurposed drugs against SARS CoV-2 main protease 3Clpro. We analyzed the molecular interactions of protein-phytocompounds, by AutoDock Vina 4.2 tools. The best interactions of each algorithm were subjected to molecular dynamic (MD) simulations to have an insight of the molecular dynamic mechanisms involved. Selected phytoconstituents gave a good score for binding affinity with the main protease 6LU7 of SARS CoV-2 as compared to the antiviral drugs already being used in the disease therapy. DehydroglyasperinC(-8.7,-8.1,-6.7,-7.1)kcal/mol, Licochalcone D(-8.4,-8.2,-7.1,-7.9) kcal/mol, Liquiritin(-8.6,-9.0,-7.2,-7.8) kcal/mol have showed energy interactions with 3Clpro better than many FDA approved repurposed drugs; Remdesvir, Favipiravir, and Hydroxychloroquine. MD Simulation also corelates our findings for molecular docking studies.
    MeSH term(s) Antiviral Agents/pharmacology ; Computer Simulation ; Coronavirus 3C Proteases/antagonists & inhibitors ; Drug Evaluation, Preclinical/methods ; Glycyrrhiza/chemistry ; Molecular Dynamics Simulation ; Plant Extracts/pharmacology ; Protein Structure, Tertiary ; SARS-CoV-2/drug effects ; SARS-CoV-2/enzymology
    Chemical Substances Antiviral Agents ; Plant Extracts ; 3C-like protease, SARS coronavirus (EC 3.4.22.-) ; Coronavirus 3C Proteases (EC 3.4.22.28)
    Language English
    Publishing date 2021-02-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 782617-5
    ISSN 1873-6351 ; 0278-6915
    ISSN (online) 1873-6351
    ISSN 0278-6915
    DOI 10.1016/j.fct.2021.112057
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    Zs.A 529: Show issues Location:
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  3. Article ; Online: New N-benzhydrylpiperazine/1,3,4-oxadiazoles conjugates inhibit the proliferation, migration, and induce apoptosis in HeLa cancer cells via oxidative stress-mediated mitochondrial pathway.

    Khanam, Rashmin / Kumar, Raj / Hejazi, Iram Iqbal / Shahabuddin, Syed / Meena, Ramovatar / Rajamani, Paulraj / Yadav, Nitin / Bhat, Asif Iqbal / Athar, Fareeda

    Journal of cellular biochemistry

    2018  Volume 120, Issue 2, Page(s) 1651–1666

    Abstract: N-benzhydrylpiperazine and 1,3,4-oxadiazoles are pharmacologically active scaffolds which exhibits significant inhibitory growth effects against various cancer cells, however, antiproliferation effects and the underlying mechanism for inducing apoptosis ... ...

    Abstract N-benzhydrylpiperazine and 1,3,4-oxadiazoles are pharmacologically active scaffolds which exhibits significant inhibitory growth effects against various cancer cells, however, antiproliferation effects and the underlying mechanism for inducing apoptosis for aforementioned scaffolds addressing HeLa cancer cells remains uncertain. In this study, N-benzhydrylpiperazine clubbed with 1,3,4-oxadiazoles (4a-4h) were synthesized, subsequently characterized using high resolution spectroscopic techniques and eventually evaluated for their antiproliferation potential by inducing apoptosis in HeLa cancer cells. The MTT assay screening results revealed that among all, compound 4d ( N-benzhydryl-4-((5-(4-aminophenyl)-1,3,4-oxadiazol-2-yl)methyl)piperazine) in particular, exhibited IC
    Language English
    Publishing date 2018-09-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 392402-6
    ISSN 1097-4644 ; 0730-2312
    ISSN (online) 1097-4644
    ISSN 0730-2312
    DOI 10.1002/jcb.27472
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1114: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article ; Online: Antioxidative and anti-proliferative potential of Curculigo orchioides Gaertn in oxidative stress induced cytotoxicity: In vitro, ex vivo and in silico studies.

    Hejazi, Iram Iqbal / Khanam, Rashmin / Mehdi, Syed Hassan / Bhat, Abdul Roouf / Rizvi, M Moshahid Alam / Thakur, Sonu Chand / Athar, Fareeda

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

    2018  Volume 115, Page(s) 244–259

    Abstract: Plant phytoconstituents have been a valuable source of clinically important anticancer agents. Antioxidant and anticancerous activity of plant Curculigo orchioides Gaertn were explored In vitro antioxidant activity, antioxidant enzyme activity of ... ...

    Abstract Plant phytoconstituents have been a valuable source of clinically important anticancer agents. Antioxidant and anticancerous activity of plant Curculigo orchioides Gaertn were explored In vitro antioxidant activity, antioxidant enzyme activity of oxidatively stressed tissue, and cell culture studies on human cancer cell lines HepG2, HeLa and MCF-7 were carried out. Active plant fractions were subjected to GC-MS analysis and compounds selected on the basis of their abundance were screened in silico with the help of Auto Dock 4.2 tools with pre-selected antioxidant enzymes. Curculigo orchioides Gaertn plant fractions exhibited significant antioxidant activities by virtue of scavenging of free radicals having IC
    Key message: Key findings explained that fractions of Curculigo orchioides Gaertn inhibited oxidative stress by increasing the antioxidant enzyme content and have anticancerous potential on cancer cell lines HepG2, HeLa and MCF-7.
    MeSH term(s) Antineoplastic Agents, Phytogenic/pharmacology ; Antioxidants/pharmacology ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Computer Simulation ; Curculigo/chemistry ; Gas Chromatography-Mass Spectrometry ; HeLa Cells ; Hep G2 Cells ; Humans ; Hydrogen Peroxide/metabolism ; Lipid Peroxidation/drug effects ; MCF-7 Cells ; Nitric Oxide/metabolism ; Oxidative Stress/drug effects ; Plant Extracts/pharmacology
    Chemical Substances Antineoplastic Agents, Phytogenic ; Antioxidants ; Plant Extracts ; Nitric Oxide (31C4KY9ESH) ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2018-03-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 782617-5
    ISSN 1873-6351 ; 0278-6915
    ISSN (online) 1873-6351
    ISSN 0278-6915
    DOI 10.1016/j.fct.2018.03.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 529: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  5. Article ; Online: Piperazine clubbed with 2-azetidinone derivatives suppresses proliferation, migration and induces apoptosis in human cervical cancer HeLa cells through oxidative stress mediated intrinsic mitochondrial pathway.

    Khanam, Rashmin / Kumar, Raj / Hejazi, Iram Iqbal / Shahabuddin, Syed / Meena, Ramovatar / Jayant, Vikrant / Kumar, Prabhat / Bhat, Abdul Roouf / Athar, Fareeda

    Apoptosis : an international journal on programmed cell death

    2018  Volume 23, Issue 2, Page(s) 113–131

    Abstract: Piperazine scaffolds or 2-azetidinone pharmacophores have been reported to show anti-cancer activities and apoptosis induction in different types of cancer cells. However, the mechanistic studies involve in induction of apoptosis addressing these two ... ...

    Abstract Piperazine scaffolds or 2-azetidinone pharmacophores have been reported to show anti-cancer activities and apoptosis induction in different types of cancer cells. However, the mechanistic studies involve in induction of apoptosis addressing these two moieties for human cervical cancer cells remain uncertain. The present study emphasizes on the anti-proliferating properties and mechanism involved in induction of apoptosis for these structurally related azoles derivatives in HeLa cancer cells. 1-Phenylpiperazine clubbed with 2-azetidione derivatives (5a-5h) were synthesized, characterized using various spectroscopic techniques and evaluated for their in-vitro anti-proliferative activities and induction of apoptosis. Further, we also evaluated oxidative stress generated by these synthetic derivatives (5a-5h). Cell viability studies revealed that among all, the compound N-(3-chloro-2-(3-nitrophenyl)-4-oxoazetidin-1-yl)-2-(4-phenylpiperazin-1-yl) acetamide 5e remarkably inhibited the growth of HeLa cells in a concentration dependent manner having IC
    MeSH term(s) Antineoplastic Agents/chemical synthesis ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Azetidines/chemical synthesis ; Azetidines/chemistry ; Azetidines/pharmacology ; Caspase 3/genetics ; Caspase 3/metabolism ; Cell Cycle Checkpoints/drug effects ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; DNA Fragmentation/drug effects ; Female ; HeLa Cells ; Humans ; Mitochondria/drug effects ; Mitochondria/genetics ; Mitochondria/metabolism ; Oxidative Stress/drug effects ; Piperazine/analogs & derivatives ; Piperazine/chemical synthesis ; Piperazine/pharmacology ; Reactive Oxygen Species/metabolism ; Uterine Cervical Neoplasms/genetics ; Uterine Cervical Neoplasms/metabolism ; Uterine Cervical Neoplasms/physiopathology
    Chemical Substances 2-azetidinone ; Antineoplastic Agents ; Azetidines ; Reactive Oxygen Species ; Piperazine (1RTM4PAL0V) ; CASP3 protein, human (EC 3.4.22.-) ; Caspase 3 (EC 3.4.22.-)
    Language English
    Publishing date 2018-01-18
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1452360-7
    ISSN 1573-675X ; 1360-8185
    ISSN (online) 1573-675X
    ISSN 1360-8185
    DOI 10.1007/s10495-018-1439-x
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5178: Show issues Location:
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  6. Article: Antioxidative and anti-proliferative potential of Curculigo orchioides Gaertn in oxidative stress induced cytotoxicity: In vitro, ex vivo and in silico studies

    Hejazi, Iram Iqbal / Abdul Roouf Bhat / Fareeda Athar / M.Moshahid Alam Rizvi / Rashmin Khanam / Sonu Chand Thakur / Syed Hassan Mehdi

    Food and chemical toxicology. 2018 May, v. 115

    2018  

    Abstract: Plant phytoconstituents have been a valuable source of clinically important anticancer agents. Antioxidant and anticancerous activity of plant Curculigo orchioides Gaertn were explored In vitro antioxidant activity, antioxidant enzyme activity of ... ...

    Abstract Plant phytoconstituents have been a valuable source of clinically important anticancer agents. Antioxidant and anticancerous activity of plant Curculigo orchioides Gaertn were explored In vitro antioxidant activity, antioxidant enzyme activity of oxidatively stressed tissue, and cell culture studies on human cancer cell lines HepG2, HeLa and MCF-7 were carried out. Active plant fractions were subjected to GC-MS analysis and compounds selected on the basis of their abundance were screened in silico with the help of Auto Dock 4.2 tools with pre-selected antioxidant enzymes. Curculigo orchioides Gaertn plant fractions exhibited significant antioxidant activities by virtue of scavenging of free radicals having IC50 value of ethylacetate fraction (EA) for DPPH radical scavenging assay to be 52.93 ± 0.66 μg/ml. Further, antioxidant enzyme defense of mammalian tissue when treated with plant fractions revealed that enzyme concentrations were refurbished which were increased during oxidative stress. MTT assay on cell lines HepG2, HeLa and MCF-7 presented IC50 values of ethylacetate (EA) fraction as 171.23 ± 2.1 μg/ml, 144.80 ± 1.08 μg/ml and 153.51 μg/ml and aqueous ethylacetate (AEA) fraction as 133.44 ± 1.1 μg/ml, 136.50 ± 0.8 μg/ml and 145.09 μg/ml respectively. Further EA and AEA plant fractions down regulated the levels of antiapoptotic Bcl-2 expression and upregulated the expression of apoptotic proteins caspase-3 and caspase-8 through an intrinsic ROS-mediated mitochondrial dysfunction pathway.Key findings explained that fractions of Curculigo orchioides Gaertn inhibited oxidative stress by increasing the antioxidant enzyme content and have anticancerous potential on cancer cell lines HepG2, HeLa and MCF-7.
    Keywords 2,2-diphenyl-1-picrylhydrazyl ; antineoplastic activity ; antineoplastic agents ; antioxidant activity ; antioxidants ; apoptosis ; caspase-3 ; caspase-8 ; cell culture ; chemical constituents of plants ; Curculigo orchioides ; cytotoxicity ; ethyl acetate ; free radicals ; gas chromatography-mass spectrometry ; human cell lines ; humans ; inhibitory concentration 50 ; mitochondria ; neoplasm cells ; neoplasms ; oxidative stress ; proteins
    Language English
    Dates of publication 2018-05
    Size p. 244-259.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 782617-5
    ISSN 1873-6351 ; 0278-6915
    ISSN (online) 1873-6351
    ISSN 0278-6915
    DOI 10.1016/j.fct.2018.03.013
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    Z 4035: Show issues

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  7. Article ; Online: New insights into the antioxidant and apoptotic potential of Glycyrrhiza glabra L. during hydrogen peroxide mediated oxidative stress: An in vitro and in silico evaluation.

    Hejazi, Iram Iqbal / Khanam, Rashmin / Mehdi, Syed Hassan / Bhat, Abdul Roouf / Moshahid Alam Rizvi, M / Islam, Asimul / Thakur, Sonu Chand / Athar, Fareeda

    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

    2017  Volume 94, Page(s) 265–279

    Abstract: Plant-derived substances (phytochemicals) are well recognized as sources of pharmacologically potent drugs in the treatment of several oxidative stress related disorders. Our study aims to evaluate the antioxidant and apoptotic effects of Glycyrrhiza ... ...

    Abstract Plant-derived substances (phytochemicals) are well recognized as sources of pharmacologically potent drugs in the treatment of several oxidative stress related disorders. Our study aims to evaluate the antioxidant and apoptotic effects of Glycyrrhiza glabra L. in both cell free and cell culture system. Plant fractions have been prepared with hexane, chloroform, ethyl acetate, methanol and water and their antioxidant properties are reviewed. Potent antioxidant activity has been well established in both in vitro and in silico studies which is believed to be responsible for the anticancerous nature of the plant. Results obtained indicate that methanol fraction of G. glabra L. exhibited maximum scavenging activity against DPPH and nitric oxide free radicals comparable to standard antioxidant L-AA. Administration of methanol fraction also considerably reduced the malondialdehyde produced due to lipid peroxidation in mammalian liver tissues. Moreover, the levels of antioxidant enzymes SOD, CAT, GST, GPx and GR in the oxidative stress induced tissues were refurbished significantly after treatment with plant's methanol fraction. Moreover, methanol fraction was found to be nontoxic to normal human cell line whereas it inhibited cancer cells HeLa and HepG2 considerably. Apoptosis was established by DAPI fluorescent staining and western blot analysis of pro apoptotic protein caspase-8, caspase-3 and anti-apoptotic protein Bcl-2.There is an up regulation in the levels of pro apoptotic caspase-8 and caspase-3 and down regulation of anti-apoptotic Bcl-2. Furthermore, GC-MS analysis of the methanol fraction revealed the presence of many compounds. In silico experiments using Autodock 4.2 tools showed strong affinity of plant compounds towards antioxidant enzymes (proteins) thus validating with the conclusions of antioxidant enzyme assays and establishing a role in cancer pathogenesis.
    Language English
    Publishing date 2017-10
    Publishing country France
    Document type Journal Article
    ZDB-ID 392415-4
    ISSN 1950-6007 ; 0753-3322 ; 0300-0893
    ISSN (online) 1950-6007
    ISSN 0753-3322 ; 0300-0893
    DOI 10.1016/j.biopha.2017.06.108
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