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  1. Artikel: Interferon-Gamma Increases the Immune Modulation of Umbilical Cord-Derived Mesenchymal Stem Cells but Decreases Their Chondrogenic Potential.

    Truong, Nhat Chau / Phan, Thu Ngoc-Minh / Huynh, Nhi Thao / Pham, Khuong Duy / Van Pham, Phuc

    Advances in experimental medicine and biology

    2023  

    Abstract: ... compared to non-treated cells (p < 0.05). Furthermore, the immunomodulatory capability of IFN-γ-treated UC ... of TGF-β compared to non-treated cells (p < 0.05).: Conclusion: This study demonstrated that UC-MSCs ...

    Abstract Introduction: The pro-inflammatory cytokine interferon-gamma (IFN-γ) is reported to be an agent that boosts the immune modulation of mesenchymal stem cells (MSCs). However, the effects of IFN-γ on the chondrogenic potential of treated MSCs have not been evaluated in depth. This study aimed to evaluate the effects of IFN-γ on the immune modulation and chondrogenic potential of human umbilical cord-derived MSCs (hUC-MSCs).
    Methods: UC-MSCs were isolated and expanded following published protocols. They were characterized as MSCs before their use in further experiments. The UC-MSCs were treated with IFN-γ at 10 ng/mL for 48 h. Changes in phenotype were investigated based on changes in MSC markers, immunomodulatory genes (TGF-β, IL-4, and IDO) for immune modulation, and cartilage-related genes during the induction of differentiation (Col1a2, Col2a1, Sox9, Runx2, and Acan) for chondrogenic potential.
    Results: IFN-γ-treated UC-MSCs maintained MSC markers and exhibited decreased expression of transcriptional regulatory factors in chondrogenesis (Sox9 and Runx2) and the extracellular matrix-specific genes Col1a2 and Acan but not Col2a1 compared to non-treated cells (p < 0.05). Furthermore, the immunomodulatory capability of IFN-γ-treated UC-MSCs was clearly revealed through their increased expression of IDO and IL-4 and decreased expression of TGF-β compared to non-treated cells (p < 0.05).
    Conclusion: This study demonstrated that UC-MSCs treated with IFN-γ at 10 ng/mL had reduced expression of chondrocyte-specific genes; however, they maintained multi-lineage differentiation and exhibited immunomodulatory properties.
    Sprache Englisch
    Erscheinungsdatum 2023-06-09
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2214-8019 ; 0065-2598
    ISSN (online) 2214-8019
    ISSN 0065-2598
    DOI 10.1007/5584_2023_776
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  2. Artikel ; Online: Duplex Imaging Assessment of the Internal Mammary Arteries in Women after Unilateral Mastectomy and Radiotherapy for Breast Cancer.

    Le, Thanh-Phong / Le, Anh T / Huynh, Tan N D / Huynh, Khanh Q / Dao, Thu-Ha / Desgranges, Pascal / Bosc, Romain

    Annals of vascular surgery

    2023  Band 100, Seite(n) 15–24

    Abstract: ... nonirradiated ones (0.180 mm [0.170, 0.200], P < 0.0001) and that of the internal mammary arteries ... in the control group (0.180 mm [0.170, 0.190], P < 0.0001). Similarly, blood flow (median [interquartile range ... nonirradiated ones (62.7 ml/min [46.87, 84.17], P < 0.0001), as well as of the left (56.7 ml/min [46.88, 72.58 ...

    Abstract Background: The effects of incidental radiation exposure on internal mammary arteries remain unclear. The present study was designed to test the hypothesis by comparing diameter and blood flow of the irradiated and nonirradiated internal mammary arteries, using Duplex ultrasound imaging.
    Methods: The study was designed as a single-center, transversal, comparative study. The main outcomes were diameter and volumetric blood flow of the internal mammary arteries. The Wilcoxon rank-sum test was used to assess the differences between the irradiated and nonirradiated internal mammary arteries with regard to the diameter and volumetric blood flow.
    Results: The diameter (median [interquartile range]) of the irradiated internal mammary arteries (0.170 mm [0.160, 0.180]) was smaller than that of the contralateral nonirradiated ones (0.180 mm [0.170, 0.200], P < 0.0001) and that of the internal mammary arteries in the control group (0.180 mm [0.170, 0.190], P < 0.0001). Similarly, blood flow (median [interquartile range]) of the irradiated internal mammary arteries (52.4 ml/min [37.78, 65.57]) was smaller than that of the contralateral nonirradiated ones (62.7 ml/min [46.87, 84.17], P < 0.0001), as well as of the left (56.7 ml/min [46.88, 72.58], P = 0.02) and the right internal mammary arteries in the control group (61.0 ml/min [47.47, 74.52], P = 0 0.0009).
    Conclusions: The data indicate that the irradiated internal mammary arteries in patients with a history of total mastectomy followed by radiotherapy for breast cancer had significantly smaller diameter and blood flow compared to the nonirradiated internal mammary arteries.
    Mesh-Begriff(e) Humans ; Female ; Breast Neoplasms/diagnostic imaging ; Breast Neoplasms/radiotherapy ; Breast Neoplasms/surgery ; Mammary Arteries/diagnostic imaging ; Mastectomy, Simple ; Mastectomy ; Treatment Outcome
    Sprache Englisch
    Erscheinungsdatum 2023-12-16
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 1027366-9
    ISSN 1615-5947 ; 0890-5096
    ISSN (online) 1615-5947
    ISSN 0890-5096
    DOI 10.1016/j.avsg.2023.10.015
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  3. Artikel ; Online: Challenges to Accurate Estimation of Methane Emission from Septic Tanks with Long Emptying Intervals.

    Moonkawin, Jakpong / Huynh, Loi T / Schneider, Mariane Y / Fujii, Shigeo / Echigo, Shinya / Nguyen, Lien P H / Hoang, Thu-Huong T / Huynh, Hai T / Harada, Hidenori

    Environmental science & technology

    2023  Band 57, Heft 43, Seite(n) 16575–16584

    Abstract: Septic tanks in low- and middle-income countries are often not emptied for a long time, potentially resulting in poor pollutant removal efficiency and increased greenhouse gas emissions, including methane ( ... ...

    Abstract Septic tanks in low- and middle-income countries are often not emptied for a long time, potentially resulting in poor pollutant removal efficiency and increased greenhouse gas emissions, including methane (CH
    Mesh-Begriff(e) Methane/analysis ; Sewage ; Greenhouse Gases ; Climate Change
    Chemische Substanzen Methane (OP0UW79H66) ; Sewage ; Greenhouse Gases
    Sprache Englisch
    Erscheinungsdatum 2023-10-19
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1520-5851
    ISSN (online) 1520-5851
    DOI 10.1021/acs.est.3c05724
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  4. Buch ; Online: Knowledge-Guided Additive Modeling For Supervised Regression

    Claes, Yann / Huynh-Thu, Vân Anh / Geurts, Pierre

    2023  

    Abstract: Learning processes by exploiting restricted domain knowledge is an important task across a plethora of scientific areas, with more and more hybrid methods combining data-driven and model-based approaches. However, while such hybrid methods have been ... ...

    Abstract Learning processes by exploiting restricted domain knowledge is an important task across a plethora of scientific areas, with more and more hybrid methods combining data-driven and model-based approaches. However, while such hybrid methods have been tested in various scientific applications, they have been mostly tested on dynamical systems, with only limited study about the influence of each model component on global performance and parameter identification. In this work, we assess the performance of hybrid modeling against traditional machine learning methods on standard regression problems. We compare, on both synthetic and real regression problems, several approaches for training such hybrid models. We focus on hybrid methods that additively combine a parametric physical term with a machine learning term and investigate model-agnostic training procedures. We also introduce a new hybrid approach based on partial dependence functions. Experiments are carried out with different types of machine learning models, including tree-based models and artificial neural networks.
    Schlagwörter Computer Science - Machine Learning
    Thema/Rubrik (Code) 006
    Erscheinungsdatum 2023-07-05
    Erscheinungsland us
    Dokumenttyp Buch ; Online
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  5. Artikel: Drug-Related Problems and Pharmacists' Interventions in Hypertensive Outpatients: A Multicenter Prospective Study in 3 Vietnamese Hospitals.

    Huynh, Bon Huu / Nathisuwan, Surakit / Tragulpiankit, Pramote / Nguyen, Vinh Dai / Huynh, Nam Huu / Vu, Thu Le Anh / Huynh, Loc Thi Hong / Vo, Ha Thi

    The Journal of pharmacy technology : jPT : official publication of the Association of Pharmacy Technicians

    2023  Band 39, Heft 6, Seite(n) 259–268

    Abstract: Background: ...

    Abstract Background:
    Sprache Englisch
    Erscheinungsdatum 2023-10-05
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 54478-4
    ISSN 8755-1225
    ISSN 8755-1225
    DOI 10.1177/87551225231199358
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  6. Artikel ; Online: Assessment of High-temperature Refined Charcoal to Improve the Safety of Grilled Meat Through the Reduction of Carcinogenic PAHs.

    Huong Huynh, Thi Thu / Tongkhao, Kullanart / Hengniran, Pongsak / Vangnai, Kanithaporn

    Journal of food protection

    2023  Band 86, Heft 12, Seite(n) 100179

    Abstract: This study presents mitigation strategies for reducing carcinogenic polycyclic aromatic hydrocarbon (PAH) contamination levels in grilled meat using high-temperature refined charcoal (HTC) prepared by Iwate kiln, also known as high-quality charcoal. Four ...

    Abstract This study presents mitigation strategies for reducing carcinogenic polycyclic aromatic hydrocarbon (PAH) contamination levels in grilled meat using high-temperature refined charcoal (HTC) prepared by Iwate kiln, also known as high-quality charcoal. Four different types of HTC were investigated for their properties in conjunction with their potential to reduce PAHs in grilled meat, including high-temperature Eucalyptus charcoal (HTEC), Leucaena charcoal (HTLC), Acacia charcoal (HTAC), and bamboo charcoal (HTBC). The results showed that all HTCs had higher fixed carbon, higher heating value, and lower volatile compounds (83.07-87.81%, 7,306-7,765 Kcal/g, and 6.98-11.97%, respectively) than commercial low-temperature refined charcoal (LTC) (65.33%, 6,728 Kcal/g, and 22.22%, respectively). The current high fixed carbon content and heating value responded to the rising maximum temperature of charcoal up to 500-600°C, providing heat source stabilization to control the radiant energy exposure of charcoal during grilling, thereby shortening grilling time. The PAH16 value of the LTC-grilled sample (144.41 μg/kg) was significantly higher than that of the HTEC, HTLC, and HTAC-grilled samples (98.21, 80.75, and 79.56 μg/kg, respectively). However, PAH16 levels in the sample grilled with HTBC were unexpectedly high (265.75 μg/kg), and cooking loss was not significantly different between samples grilled with all charcoals. Overall, the findings indicated that using HTC prepared from Eucalyptus, Leucaena, and Acacia woods could reduce PAH contamination in grilled pork by up to 45%. However, more research is needed to determine the best preparation method for high-quality charcoal made from bamboo.
    Mesh-Begriff(e) Carcinogens/analysis ; Charcoal ; Temperature ; Meat/analysis ; Cooking/methods ; Polycyclic Aromatic Hydrocarbons/analysis
    Chemische Substanzen Carcinogens ; Charcoal (16291-96-6) ; Polycyclic Aromatic Hydrocarbons
    Sprache Englisch
    Erscheinungsdatum 2023-10-13
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 243284-5
    ISSN 1944-9097 ; 0362-028X
    ISSN (online) 1944-9097
    ISSN 0362-028X
    DOI 10.1016/j.jfp.2023.100179
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  7. Artikel ; Online: Use of enzyme-linked immunosorbent assay to screen for aflatoxins, ochratoxin A, and deoxynivalenol in dry pet foods.

    Okuma, Tara A / Huynh, Thu P / Hellberg, Rosalee S

    Mycotoxin research

    2017  Band 34, Heft 1, Seite(n) 69–75

    Abstract: The objective of this study was to perform a market survey on dry pet foods using enzyme-linked immunosorbent assay (ELISA) to detect total aflatoxins (AFs), ochratoxin A (OTA), and deoxynivalenol (DON). Pet food products (n = 58) marketed for dogs, cats, ...

    Abstract The objective of this study was to perform a market survey on dry pet foods using enzyme-linked immunosorbent assay (ELISA) to detect total aflatoxins (AFs), ochratoxin A (OTA), and deoxynivalenol (DON). Pet food products (n = 58) marketed for dogs, cats, birds, and rabbits were tested in duplicate with ELISA, and results above the limit of quantitation were confirmed using liquid chromatography tandem mass spectrometry (LC-MS/MS). OTA was detected in one product (rabbit food) and AFs were detected in two products (one dog treat and one bird treat). In contrast, DON was detected in the majority (74%) of products tested. Bird and rabbit products were the most affected by DON, with levels above 0.5 μg/g in 50 and 80% of samples, respectively. One rabbit sample tested positive for both OTA and DON. Overall, the findings of this study revealed a low incidence of AFs and OTA in commercial pet food. Although DON was detected in numerous products, the levels were well below those associated with acute toxic effects.
    Mesh-Begriff(e) Aflatoxins/analysis ; Animal Feed/analysis ; Chromatography, Liquid ; Enzyme-Linked Immunosorbent Assay/methods ; Food Analysis/methods ; Ochratoxins/analysis ; Poisons/analysis ; Tandem Mass Spectrometry ; Trichothecenes/analysis
    Chemische Substanzen Aflatoxins ; Ochratoxins ; Poisons ; Trichothecenes ; ochratoxin A (1779SX6LUY) ; deoxynivalenol (JT37HYP23V)
    Sprache Englisch
    Erscheinungsdatum 2017-11-16
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 14500-2
    ISSN 1867-1632 ; 0178-7888
    ISSN (online) 1867-1632
    ISSN 0178-7888
    DOI 10.1007/s12550-017-0300-3
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  8. Artikel ; Online: Improved Sample Selection and Preparation Methods for Sampling Plans Used to Facilitate Rapid and Reliable Estimation of Aflatoxin in Chicken Feed

    James Kibugu / Raymond Mdachi / Leonard Munga / David Mburu / Thomas Whitaker / Thu P. Huynh / Delia Grace / Johanna F. Lindahl

    Toxins, Vol 13, Iss 216, p

    2021  Band 216

    Abstract: Aflatoxin B1 (AFB1), a toxic fungal metabolite associated with human and animal diseases, is a natural contaminant encountered in agricultural commodities, food and feed. Heterogeneity of AFB1 makes risk estimation a challenge. To overcome this, novel ... ...

    Abstract Aflatoxin B1 (AFB1), a toxic fungal metabolite associated with human and animal diseases, is a natural contaminant encountered in agricultural commodities, food and feed. Heterogeneity of AFB1 makes risk estimation a challenge. To overcome this, novel sample selection, preparation and extraction steps were designed for representative sampling of chicken feed. Accuracy, precision, limits of detection and quantification, linearity, robustness and ruggedness were used as performance criteria to validate this modification and Horwitz function for evaluating precision. A modified sampling protocol that ensured representativeness is documented, including sample selection, sampling tools, random procedures, minimum size of field-collected aggregate samples (primary sampling), procedures for mass reduction to 2 kg laboratory (secondary sampling), 25 g test portion (tertiary sampling) and 1.3 g analytical samples (quaternary sampling). The improved coning and quartering procedure described herein (for secondary and tertiary sampling) has acceptable precision, with a Horwitz ratio (HorRat = 0.3) suitable for splitting of 25 g feed aliquots from laboratory samples (tertiary sampling). The water slurring innovation (quaternary sampling) increased aflatoxin extraction efficiency to 95.1% through reduction of both bias (−4.95) and variability of recovery (1.2–1.4) and improved both intra-laboratory precision (HorRat = 1.2–1.5) and within-laboratory reproducibility (HorRat = 0.9–1.3). Optimal extraction conditions are documented. The improved procedure showed satisfactory performance, good field applicability and reduced sample analysis turnaround time.
    Schlagwörter aflatoxin ; chicken feed ; representative sampling ; improved aflatoxin test procedure ; validation ; Medicine ; R
    Thema/Rubrik (Code) 310
    Sprache Englisch
    Erscheinungsdatum 2021-03-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
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  9. Artikel ; Online: Creating an appropriate tenure foundation for REDD+

    Sunderlin, W.D. / Sassi, Claudio de / Sills, E.O. / Duchelle, A.E. / Larson, A.M. / Resosudarmo, I.A.P. / Awono, A. / Kweka, D. / Huynh Thu Ba

    The record to date and prospects for the future

    2021  

    Schlagwörter forests ; deforestation ; climate change ; tenure ; developing countries
    Sprache Englisch
    Erscheinungsdatum 2021-03-08T08:31:54Z
    Verlag Elsevier
    Erscheinungsland fr
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  10. Artikel ; Online: Improved sample selection and preparation methods for sampling plans used to facilitate rapid and reliable estimation of aflatoxin in chicken feed

    Kibugu, J. / Mdachi, R. / Munga, L. / Mburu, D. / Whitaker, T. / Thu P. Huynh / Grace, Delia / Lindahl, Johanna F.

    Toxins

    2021  

    Abstract: Aflatoxin B1 (AFB1), a toxic fungal metabolite associated with human and animal diseases, is a natural contaminant encountered in agricultural commodities, food and feed. Heterogeneity of AFB1 makes risk estimation a challenge. To overcome this, novel ... ...

    Abstract Aflatoxin B1 (AFB1), a toxic fungal metabolite associated with human and animal diseases, is a natural contaminant encountered in agricultural commodities, food and feed. Heterogeneity of AFB1 makes risk estimation a challenge. To overcome this, novel sample selection, preparation and extraction steps were designed for representative sampling of chicken feed. Accuracy, precision, limits of detection and quantification, linearity, robustness and ruggedness were used as performance criteria to validate this modification and Horwitz function for evaluating precision. A modified sampling protocol that ensured representativeness is documented, including sample selection, sampling tools, random procedures, minimum size of field-collected aggregate samples (primary sampling), procedures for mass reduction to 2 kg laboratory (secondary sampling), 25 g test portion (tertiary sampling) and 1.3 g analytical samples (quaternary sampling). The improved coning and quartering procedure described herein (for secondary and tertiary sampling) has acceptable precision, with a Horwitz ratio (HorRat = 0.3) suitable for splitting of 25 g feed aliquots from laboratory samples (tertiary sampling). The water slurring innovation (quaternary sampling) increased aflatoxin extraction efficiency to 95.1% through reduction of both bias (−4.95) and variability of recovery (1.2–1.4) and improved both intra-laboratory precision (HorRat = 1.2–1.5) and within-laboratory reproducibility (HorRat = 0.9–1.3). Optimal extraction conditions are documented. The improved procedure showed satisfactory performance, good field applicability and reduced sample analysis turnaround time.
    Schlagwörter aflatoxins ; feeds ; poultry ; chickens ; toxicology
    Sprache Englisch
    Erscheinungsdatum 2021-03-18T07:13:17Z
    Verlag MDPI
    Erscheinungsland fr
    Dokumenttyp Artikel ; Online
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