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  1. Article ; Online: Robot Dance

    Luis Gustavo Nonato / Pedro Peixoto / Tiago Pereira / Claudia Sagastizábal / Paulo J.S. Silva

    EURO Journal on Computational Optimization, Vol 10, Iss , Pp 100025- (2022)

    A mathematical optimization platform for intervention against COVID-19 in a complex network

    2022  

    Abstract: Robot Dance is a computational optimization platform developed in response to the COVID-19 outbreak, to support the decision-making on public policies at a regional level. The tool is suitable for understanding and suggesting levels of intervention ... ...

    Abstract Robot Dance is a computational optimization platform developed in response to the COVID-19 outbreak, to support the decision-making on public policies at a regional level. The tool is suitable for understanding and suggesting levels of intervention needed to contain the spread of infectious diseases when the mobility of inhabitants through a regional network is a concern. Such is the case for the SARS-CoV-2 virus that is highly contagious and, therefore, makes it crucial to incorporate the circulation of people in the epidemiological compartmental models. Robot Dance anticipates the spread of an epidemic in a complex regional network, helping to identify fragile links where applying differentiated measures of containment, testing, and vaccination is important. Based on stochastic optimization, the model determines efficient strategies on the basis of commuting of individuals and the situation of hospitals in each district. Uncertainty in the capacity of intensive care beds is handled by a chance-constraint approach. Some functionalities of Robot Dance are illustrated in the state of São Paulo in Brazil, using real data for a region with more than forty million inhabitants.
    Keywords 90C15 ; 49-04 ; 92C60 ; Applied mathematics. Quantitative methods ; T57-57.97 ; Electronic computers. Computer science ; QA75.5-76.95
    Subject code 629
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Entre el opio del pueblo y la búsqueda de la salvación. Aproximaciones a la religiosidad vivida desde América Latina

    Valentina Pereira Arena / Gustavo Morello S. J.

    Revista de Estudios Sociales, Vol 82, Pp 3-

    2022  Volume 21

    Abstract: In this special issue, we explore the concept of lived religion in order to construct categories for the study of religion that reflect the Latin American reality, where, despite modernization, studies do not observe secularization, but rather a ... ...

    Abstract In this special issue, we explore the concept of lived religion in order to construct categories for the study of religion that reflect the Latin American reality, where, despite modernization, studies do not observe secularization, but rather a transformation in the practices and social role of religion. Lived religion deals with understanding what ordinary people do when they practice religion in their daily lives. When we speak of lived religion, we take a serious approach to what the participants understand religion to be: it is the people who practice it who define what religion is and what function it fulfills in their lives. The authors of this issue understand lived religion as the practices that ordinary people perform in everyday life situations to connect with superhuman powers. These incorporate corporeality, materiality, and discourse, and are chosen by individuals from a religious cultural repertoire. The articles in this issue are based on empirical research on animitas, processions, domestic altars, spaces of commercialization of religious objects, interviews and surveys that collect religious practices, and cases of religious everyday life in Mexico, Colombia, Chile and Peru. On this basis, they expand, problematize and discuss the approach of lived religion, with alternatives such as hinge religoisity or lived spirituality. At the same time, they show the limits of the theories of secularization and the religious market to explain Latin American religious dynamics.
    Keywords corporality and religion ; hinge religiosity ; latin america ; lived religion ; lived spirituality ; materiality and religious objects ; popular religiosity ; religious consumption ; religious market ; religious practice ; secularization ; Social Sciences ; H ; Social sciences (General) ; H1-99
    Subject code 290 ; 200
    Language English
    Publishing date 2022-10-01T00:00:00Z
    Publisher Universidad de los Andes
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Two-pore channel-2 and inositol trisphosphate receptors coordinate Ca

    Yuan, Yu / Arige, Vikas / Saito, Ryo / Mu, Qianru / Brailoiu, Gabriela C / Pereira, Gustavo J S / Bolsover, Stephen R / Keller, Marco / Bracher, Franz / Grimm, Christian / Brailoiu, Eugen / Marchant, Jonathan S / Yule, David I / Patel, Sandip

    Cell reports

    2023  Volume 43, Issue 1, Page(s) 113628

    Abstract: Lysosomes and the endoplasmic reticulum (ER) are ... ...

    Abstract Lysosomes and the endoplasmic reticulum (ER) are Ca
    MeSH term(s) Two-Pore Channels ; Calcium Signaling/physiology ; Inositol/metabolism ; Endoplasmic Reticulum/metabolism ; Lysosomes/metabolism ; Calcium/metabolism ; NADP/metabolism ; Inositol 1,4,5-Trisphosphate Receptors/metabolism ; Inositol 1,4,5-Trisphosphate
    Chemical Substances Two-Pore Channels ; Inositol (4L6452S749) ; Calcium (SY7Q814VUP) ; NADP (53-59-8) ; Inositol 1,4,5-Trisphosphate Receptors ; Inositol 1,4,5-Trisphosphate (85166-31-0)
    Language English
    Publishing date 2023-12-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2649101-1
    ISSN 2211-1247 ; 2211-1247
    ISSN (online) 2211-1247
    ISSN 2211-1247
    DOI 10.1016/j.celrep.2023.113628
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Two-pore channel-2 and inositol trisphosphate receptors coordinate Ca2+ signals between lysosomes and the endoplasmic reticulum

    Yu Yuan / Vikas Arige / Ryo Saito / Qianru Mu / Gabriela C. Brailoiu / Gustavo J.S. Pereira / Stephen R. Bolsover / Marco Keller / Franz Bracher / Christian Grimm / Eugen Brailoiu / Jonathan S. Marchant / David I. Yule / Sandip Patel

    Cell Reports, Vol 43, Iss 1, Pp 113628- (2024)

    2024  

    Abstract: Summary: Lysosomes and the endoplasmic reticulum (ER) are Ca2+ stores mobilized by the second messengers NAADP and IP3, respectively. Here, we establish Ca2+ signals between the two sources as fundamental building blocks that couple local release to ... ...

    Abstract Summary: Lysosomes and the endoplasmic reticulum (ER) are Ca2+ stores mobilized by the second messengers NAADP and IP3, respectively. Here, we establish Ca2+ signals between the two sources as fundamental building blocks that couple local release to global changes in Ca2+. Cell-wide Ca2+ signals evoked by activation of endogenous NAADP-sensitive channels on lysosomes comprise both local and global components and exhibit a major dependence on ER Ca2+ despite their lysosomal origin. Knockout of ER IP3 receptor channels delays these signals, whereas expression of lysosomal TPC2 channels accelerates them. High-resolution Ca2+ imaging reveals elementary events upon TPC2 opening and signals coupled to IP3 receptors. Biasing TPC2 activation to a Ca2+-permeable state sensitizes local Ca2+ signals to IP3. This increases the potency of a physiological agonist to evoke global Ca2+ signals and activate a downstream target. Our data provide a conceptual framework to understand how Ca2+ release from physically separated stores is coordinated.
    Keywords CP: Cell biology ; Biology (General) ; QH301-705.5
    Subject code 572
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Influence of Suspended Solid Particles on Calcium Carbonate Fouling In Dripper Labyrinths

    Muniz, Gustavo L. / Camargo, Antonio P. / Signorelli, Filipe / Bertran, Celso A. / Pereira, Diego J.S. / Frizzone, José A.

    Agricultural water management. 2022,

    2022  

    Abstract: Calcium carbonate (CaCO₃) fouling process in dripper prototypes was studied in the absence and presence of suspended solid particles (SSP) at concentrations of 125 and 500mgL⁻ᵍ. Eleven flat and non-pressure-compensating dripper prototypes were evaluated. ...

    Abstract Calcium carbonate (CaCO₃) fouling process in dripper prototypes was studied in the absence and presence of suspended solid particles (SSP) at concentrations of 125 and 500mgL⁻ᵍ. Eleven flat and non-pressure-compensating dripper prototypes were evaluated. Five prototypes (Model A) present flow with well-developed vortices, whereas the other six prototypes (Model B) present more uniform flow with undeveloped vortices. Water with hardness of 200 and 300mgL⁻ᵍ as CaCO₃ was used in the experiments. The dripper prototypes operated for 200h, and the discharge variation rate (Dra) was determined every 40h. Images of the interior of the labyrinths were acquired to investigate the fouling patterns. The mass of material fouling the labyrinth of the prototypes was determined and correlated to the geometric parameters of the labyrinths. The deposited material was analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The SSP directly influenced the CaCO₃ fouling processes. In the absence of SSP, heterogeneous nucleation was dominant in the prototype walls and occurred uniformly throughout the labyrinth, interfering directly in the Dra of the model B prototypes. The model A prototypes were more resistant to clogging and did not clog during the experiments. Once added to the water, the SSP functioned as an effective competitor of CaCO₃ nucleation and growth sites; thus, heterogeneous nucleation started occurring preferably on the SSP surface, reducing the fouling in the labyrinth walls. The prototypes that clogged in the tests in the absence of SSP were resistant to clogging in the SSP concentration of 500mgL⁻ᵍ. The presence of SSP changed the crystal habit of CaCO₃, and it precipitated in the form of calcite. The nucleation of CaCO₃ directly on the SSP surface is interesting from the point of view of clogging processes, and drippers with better sediment transport capacity are beneficial.
    Keywords X-ray diffraction ; calcite ; calcium carbonate ; geometry ; hardness ; models ; prototypes ; sediment transport ; total suspended solids ; water management
    Language English
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 751144-9
    ISSN 1873-2283 ; 0378-3774
    ISSN (online) 1873-2283
    ISSN 0378-3774
    DOI 10.1016/j.agwat.2022.107890
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Artemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca

    Zamarioli, Lucas Dos Santos / Santos, Michele Rosana Maia / Erustes, Adolfo Garcia / Meccatti, Vanessa Marques / Pereira, Thaís Cristine / Smaili, Soraya S / Marcucci, Maria Cristina / Oliveira, Carlos Rocha / Pereira, Gustavo J S / Bincoletto, Claudia

    Chinese journal of integrative medicine

    2023  

    Abstract: Objective: To evaluate the chemical composition and effects of Artemisia vulgaris (AV) hydroalcoholic extract (HEAV) on breast cancer cells (MCF-7 and SKBR-3), chronic myeloid leukemia (K562) and NIH/3T3 fibroblasts.: Methods: Phytochemical analysis ... ...

    Abstract Objective: To evaluate the chemical composition and effects of Artemisia vulgaris (AV) hydroalcoholic extract (HEAV) on breast cancer cells (MCF-7 and SKBR-3), chronic myeloid leukemia (K562) and NIH/3T3 fibroblasts.
    Methods: Phytochemical analysis of HEAV was done by high-performance liquid chromatography-mass (HPLC) spectrometry. Viability and cell death studies were performed using trypan blue and Annexin/FITC-7AAD, respectively. Ferrostatin-1 (Fer-1) and necrostatin-1 (Nec-1) were used to assess the mode of HEAV-induced cell death and acetoxymethylester (BAPTA-AM) was used to verify the involvement of cytosolic calcium in this event. Cytosolic calcium measurements were made using Fura-2-AM.
    Results: HEAV decreased the viability of MCF-7, SKBR-3 and K562 cells (P<0.05). The viability of HEAV-treated K562 cells was reduced compared to HEAV-exposed fibroblasts (P<0.05). Treatment of K562 cells with HEAV induced cell death primarily by late apoptosis and necrosis in assays using annexin V-FITC/7-AAD (P<0.05). The use of Nec-1 and Fer-1 increased the viability of K562 cells treated with HEAV relative to cells exposed to HEAV alone (P<0.01). HEAV-induced Ca
    Conclusions: HEAV is cytotoxic and activates several modalities of cell death, which are partially dependent on lysosomal release of Ca
    Language English
    Publishing date 2023-12-02
    Publishing country China
    Document type Journal Article
    ZDB-ID 2171254-2
    ISSN 1993-0402 ; 1672-0415
    ISSN (online) 1993-0402
    ISSN 1672-0415
    DOI 10.1007/s11655-023-3712-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Optimisation of the filter housing dimensions of an automatic flushing strainer-type filter

    Cano, Nicolas D. / Camargo, Antonio P. / Ait-Mouheb, Nassim / Muniz, Gustavo L. / Guarnizo, Jhonnatan A.Y. / Pereira, Diego J.S. / Frizzone, José A.

    IAgrE Biosystems engineering. 2022 July, v. 219

    2022  

    Abstract: Filtration is essential in drip irrigation systems to remove physical contaminants carried by water that can clog the emitters, cause wear, or foul components of the system, affecting its performance and lifespan. Automatic flushing strainer-type filters ...

    Abstract Filtration is essential in drip irrigation systems to remove physical contaminants carried by water that can clog the emitters, cause wear, or foul components of the system, affecting its performance and lifespan. Automatic flushing strainer-type filters initiate and terminate discrete flushing cycles that are activated automatically by means of differential pressure. The objective of this study is to investigate the hydraulic performance and flow behaviour of an automatic flushing strainer-type filter operated with clean water, using experimental and numerical approaches, to optimise the dimensions of its filter housing and to increase the range of operating flow rates. Pressure drop curves were determined for the filter housing and the filter system equipped with five models of filter elements (woven and non-woven elements). Excessive pressure drop in the filter housing and low filtration rates were identified as the main drawbacks of the original filter system. Numerical simulations enabled the identification of the most critical regions in terms of pressure losses near the transitions between the inlet and outlet segments of the pipe. Four designs of filter housing were simulated to evaluate the possibilities of optimising the filter housing dimensions using a constant filtering area. Larger inlet and outlet diameters combined to a filter housing shorter and wider were improvements in the filter housing dimensions that enabled to decrease the pressure drop in the filter and/or increase the range of operating flow rates. The results provided useful information for enhancing the hydraulic performance of the filtration system.
    Keywords differential pressure ; filtration ; longevity ; microirrigation
    Language English
    Dates of publication 2022-07
    Size p. 25-37.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2075942-3
    ISSN 1537-5110
    ISSN 1537-5110
    DOI 10.1016/j.biosystemseng.2022.04.019
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Palladium (II) compounds containing oximes as promising antitumor agents for the treatment of osteosarcoma: An in vitro and in vivo comparative study with cisplatin.

    Pereira, Thales Hebert Regiani / de Moura, Thales Reggiani / Santos, Michele Rosana Maia / Zamarioli, Lucas Dos Santos / Erustes, Adolfo G / Smaili, Soraya S / Pereira, Gustavo J S / Godoy Netto, Adelino Vieira de / Bincoletto, Claudia

    European journal of medicinal chemistry

    2023  Volume 264, Page(s) 116034

    Abstract: Drug resistance, evasion of cell death and metastasis are factors that contribute to the low cure rate and disease-free survival in osteosarcomas (OS). In this study, we demonstrated that a new class of oxime-containing organometallic complexes called Pd- ...

    Abstract Drug resistance, evasion of cell death and metastasis are factors that contribute to the low cure rate and disease-free survival in osteosarcomas (OS). In this study, we demonstrated that a new class of oxime-containing organometallic complexes called Pd-BPO (O3) and Pd-BMO (O4) are more cytotoxic than cisplatin (CDDP) for SaOS-2 and U2OS cells using the MTT assay. Annexin-FITC/7-AAD staining demonstrated a greater potential for palladium-oxime complexes to induce death in SaOS-2 cells than CDDP, an event confirmed using the pan-caspase inhibitor Z-VAD-FMK. Compared to CDDP, only palladium-oxime complexes eradicated the clonogenicity of SaOS-2 cells after 7 days of treatment. The involvement of the lysosome-mitochondria axis in the cell death-inducing properties of the complexes was also evaluated. Using LysoTracker Red to label the acidic organelles of SaOS-2 cells treated with the O3 and O4 complexes, a decrease in the fluorescence intensity of this probe was observed in relation to CDDP and the control. Lysosomal membrane permeabilization (LMP) was also induced by the O3 and O4 complexes in an assay using acridine orange (A/O). The greater efficiency of the complexes in depolarizing the mitochondrial membrane compared to SaOS-2 cells treated with CDDP was also observed using TMRE (tetramethyl rhodamine, ethyl ester). For in vivo studies, C. elegans was used and demonstrated that both complexes reduce body bends and pharyngeal pumping after 24 h of treatment to the same extent as CDDP. We conclude that both palladium-oxime complexes are more effective than CDDP in inducing tumor cell death. The toxicity of these complexes to C. elegans was like that induced by CDDP. These results encourage preclinical studies aimed at developing more effective drugs for the treatment of osteosarcoma (OS). Furthermore, we propose palladium-oxime complexes as a new class of antineoplastic agents.
    MeSH term(s) Animals ; Humans ; Cisplatin/pharmacology ; Palladium/pharmacology ; Caenorhabditis elegans ; Apoptosis ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Osteosarcoma/pathology ; Bone Neoplasms/pathology ; Cell Line, Tumor
    Chemical Substances Cisplatin (Q20Q21Q62J) ; Palladium (5TWQ1V240M) ; Antineoplastic Agents
    Language English
    Publishing date 2023-12-11
    Publishing country France
    Document type Journal Article
    ZDB-ID 188597-2
    ISSN 1768-3254 ; 0009-4374 ; 0223-5234
    ISSN (online) 1768-3254
    ISSN 0009-4374 ; 0223-5234
    DOI 10.1016/j.ejmech.2023.116034
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Blocking Autophagy by the Two-Pore Channels Antagonist Tetrandrine Improves Sorafenib-Induced Death of Hepatocellular Carcinoma Cells.

    Sperandio, Letícia Paulino / Lins, Isis Valeska F / Erustes, Adolfo G / Leão, Anderson H F F / Antunes, Fernanda / Morais, Ingrid B M / Vieira, Heron Fernandes / de Campos, Laís Maria / Bincoletto, Claudia / Smaili, Soraya S / Pereira, Gustavo J S

    Toxicology in vitro : an international journal published in association with BIBRA

    2023  Volume 90, Page(s) 105603

    Abstract: Sorafenib, an oral multi-kinase inhibitor, used to treat hepatocellular carcinoma (HCC). However, drug resistance is still common in several HCC patients. This complex mechanism is not yet fully elucidated, driving the search for new therapeutic targets ... ...

    Abstract Sorafenib, an oral multi-kinase inhibitor, used to treat hepatocellular carcinoma (HCC). However, drug resistance is still common in several HCC patients. This complex mechanism is not yet fully elucidated, driving the search for new therapeutic targets to potentiate the antitumoral effect of sorafenib. Recent findings have linked the expression of Two-Pore Channels (TPCs) receptors with the development and progression of cancer. TPCs receptors are stimulated by NAADP, a Ca
    MeSH term(s) Humans ; Sorafenib/pharmacology ; Carcinoma, Hepatocellular/drug therapy ; Carcinoma, Hepatocellular/metabolism ; Liver Neoplasms/metabolism ; Drug Resistance, Neoplasm ; Cell Line, Tumor ; Autophagy
    Chemical Substances Sorafenib (9ZOQ3TZI87) ; tetrandrine (29EX23D5AJ)
    Language English
    Publishing date 2023-04-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 639064-x
    ISSN 1879-3177 ; 0887-2333
    ISSN (online) 1879-3177
    ISSN 0887-2333
    DOI 10.1016/j.tiv.2023.105603
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Optimized delay of the second COVID-19 vaccine dose reduces ICU admissions.

    Silva, Paulo J S / Sagastizábal, Claudia / Nonato, Luís Gustavo / Struchiner, Claudio José / Pereira, Tiago

    Proceedings of the National Academy of Sciences of the United States of America

    2021  Volume 118, Issue 35

    Abstract: Slower than anticipated, COVID-19 vaccine production and distribution have impaired efforts to curtail the current pandemic. The standard administration schedule for most COVID-19 vaccines currently approved is two doses administered 3 to 4 wk apart. To ... ...

    Abstract Slower than anticipated, COVID-19 vaccine production and distribution have impaired efforts to curtail the current pandemic. The standard administration schedule for most COVID-19 vaccines currently approved is two doses administered 3 to 4 wk apart. To increase the number of individuals with partial protection, some governments are considering delaying the second vaccine dose. However, the delay duration must take into account crucial factors, such as the degree of protection conferred by a single dose, the anticipated vaccine supply pipeline, and the potential emergence of more virulent COVID-19 variants. To help guide decision-making, we propose here an optimization model based on extended susceptible, exposed, infectious, and removed (SEIR) dynamics that determines the optimal delay duration between the first and second COVID-19 vaccine doses. The model assumes lenient social distancing and uses intensive care unit (ICU) admission as a key metric while selecting the optimal duration between doses vs. the standard 4-wk delay. While epistemic uncertainties apply to the interpretation of simulation outputs, we found that the delay is dependent on the vaccine mechanism of action and first-dose efficacy. For infection-blocking vaccines with first-dose efficacy ≥50%, the model predicts that the second dose can be delayed by ≥8 wk (half of the maximal delay), whereas for symptom-alleviating vaccines, the same delay is recommended only if the first-dose efficacy is ≥70%. Our model predicts that a 12-wk second-dose delay of an infection-blocking vaccine with a first-dose efficacy ≥70% could reduce ICU admissions by 400 people per million over 200 d.
    MeSH term(s) Algorithms ; Brazil/epidemiology ; COVID-19/epidemiology ; COVID-19/immunology ; COVID-19/prevention & control ; COVID-19 Vaccines/administration & dosage ; COVID-19 Vaccines/supply & distribution ; Hospitalization/statistics & numerical data ; Humans ; Intensive Care Units/statistics & numerical data ; SARS-CoV-2/immunology ; Time-to-Treatment/standards ; Treatment Outcome ; Vaccination/methods ; Vaccination/statistics & numerical data
    Chemical Substances COVID-19 Vaccines
    Language English
    Publishing date 2021-08-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2104640118
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