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  1. Article ; Online: Assessing the cost-effectiveness of Nature-based Solutions under climate change uncertainty and learning

    Vogelsang, Lennart G. / Weikard, Hans Peter / van Loon-Steensma, Jantsje M. / Bednar-Friedl, Birgit

    Water Resources and Economics

    2023  Volume 43

    Abstract: While ongoing climate change requires effective adaptation strategies, decision-making on the scale, timing and location of adaptation measures remains challenging, as the extent and pace of climate change is uncertain. In particular, uncertainty ... ...

    Abstract While ongoing climate change requires effective adaptation strategies, decision-making on the scale, timing and location of adaptation measures remains challenging, as the extent and pace of climate change is uncertain. In particular, uncertainty surrounds the success of mitigation strategies and economic and demographic developments. While ‘grey’ measures, such as dikes and pumping, have been the dominant form of adaptation in the past against inland flooding, Nature-based Solutions are receiving more attention. However, evidence on their cost-effectiveness compared to grey alternatives is still limited. In this article, we develop a real options model that integrates climate change uncertainty as well as flexibility of the timing of investment. We expect that the uncertainty falls over time due to an increase in climate data that becomes available to the decision-maker. The decision-maker learns about climate change from two types of events: inland flooding and meteorological drought. The model is calibrated with data from the Oldambt–Eemskanaal–Dollardboezem water system in the Dutch Province of Groningen. Our results show the potential for Nature-based Solutions to become a viable alternative to dike heightening and pumping in addressing both extreme events. Moreover, we observe that the cost-effectiveness of Nature-based Solutions depends on the maturing time of the ecosystem, the value of land on which the measure is being implemented, and the type of information considered in the investment decision. We show that maturing times of up to nine years make a Nature-based Solution the preferred measure to invest in, even under strong beliefs of severe climate change. When maturing times exceed nine years, the option takes too long to become effective and pumping is preferred. Furthermore, our results show that an increase in the value of land on which a Nature-based Solution is being constructed may substantially reduce its cost-effectiveness. Finally, decision-making flexibility becomes valuable if expected ...
    Keywords Drought ; Flood ; Nature-based solutions ; Real options analysis
    Subject code 650
    Language English
    Publishing country nl
    Document type Article ; Online
    ISSN 2212-4284
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice.

    Schroeter, Christina B / Henes, Antonia / Vogelsang, Anna / Herrmann, Alexander M / Lichtenberg, Stefanie / Cengiz, Derya / Dobelmann, Vera / Huntemann, Niklas / Nelke, Christopher / Eichler, Susann / Albrecht, Philipp / Meuth, Sven G / Ruck, Tobias

    Journal of visualized experiments : JoVE

    2023  , Issue 200

    Abstract: Experimental autoimmune encephalomyelitis (EAE) is the most common murine model for multiple sclerosis (MS) and is frequently used to further elucidate the still unknown etiology of MS in order to develop new treatment strategies. The myelin ... ...

    Abstract Experimental autoimmune encephalomyelitis (EAE) is the most common murine model for multiple sclerosis (MS) and is frequently used to further elucidate the still unknown etiology of MS in order to develop new treatment strategies. The myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55) EAE model reproduces a self-limiting monophasic disease course with ascending paralysis within 10 days after immunization. The mice are examined daily using a clinical scoring system. MS is driven by different pathomechanisms with a specific temporal pattern, thus the investigation of the role of central nervous system (CNS)-resident cell types during disease progression is of great interest. The unique feature of this protocol is the simultaneous isolation of all principal CNS-resident cell types (microglia, oligodendrocytes, astrocytes, and neurons) applicable in adult EAE and healthy mice. The dissociation of the brain and the spinal cord from adult mice is followed by magnetic-activated cell sorting (MACS) to isolate microglia, oligodendrocytes, astrocytes, and neurons. Flow cytometry was used to perform quality analyses of the purified single-cell suspensions confirming viability after cell isolation and indicating the purity of each cell type of approximately 90%. In conclusion, this protocol offers a precise and comprehensive way to analyze complex cellular networks in healthy and EAE mice. Moreover, required mice numbers can be substantially reduced as all four cell types are isolated from the same mice.
    MeSH term(s) Mice ; Animals ; Encephalomyelitis, Autoimmune, Experimental/etiology ; Mice, Inbred C57BL ; Central Nervous System/metabolism ; Multiple Sclerosis ; Spinal Cord/metabolism ; Myelin-Oligodendrocyte Glycoprotein ; Encephalomyelitis/complications ; Peptide Fragments
    Chemical Substances Myelin-Oligodendrocyte Glycoprotein ; Peptide Fragments
    Language English
    Publishing date 2023-10-06
    Publishing country United States
    Document type Journal Article ; Video-Audio Media ; Research Support, Non-U.S. Gov't
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/65735
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: One Brain-All Cells: A Comprehensive Protocol to Isolate All Principal CNS-Resident Cell Types from Brain and Spinal Cord of Adult Healthy and EAE Mice.

    Schroeter, Christina B / Herrmann, Alexander M / Bock, Stefanie / Vogelsang, Anna / Eichler, Susann / Albrecht, Philipp / Meuth, Sven G / Ruck, Tobias

    Cells

    2021  Volume 10, Issue 3

    Abstract: In experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, the role of each central nervous system (CNS)-resident cell type during inflammation, neurodegeneration, and remission has been frequently addressed. Although ... ...

    Abstract In experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, the role of each central nervous system (CNS)-resident cell type during inflammation, neurodegeneration, and remission has been frequently addressed. Although protocols for the isolation of different individual CNS-resident cell types exist, none can harvest all of them within a single experiment. In addition, isolation of individual cells is more demanding in adult mice and even more so from the inflamed CNS. Here, we present a protocol for the simultaneous purification of viable single-cell suspensions of all principal CNS-resident cell types (microglia, oligodendrocytes, astrocytes, and neurons) from adult mice-applicable in healthy mice as well as in EAE. After dissociation of the brain and spinal cord from adult mice, microglia, oligodendrocytes, astrocytes and, neurons were isolated via magnetic-activated cell sorting (MACS). Validations comprised flow cytometry, immunocytochemistry, as well as functional analyses (immunoassay and Sholl analysis). The purity of each cell isolation averaged 90%. All cells displayed cell-type-specific morphologies and expressed specific surface markers. In conclusion, this new protocol for the simultaneous isolation of all major CNS-resident cell types from one CNS offers a sophisticated and comprehensive way to investigate complex cellular networks ex vivo and simultaneously reduce mice numbers to be sacrificed.
    MeSH term(s) Animals ; Brain/cytology ; Brain/metabolism ; Cell Separation ; Encephalomyelitis, Autoimmune, Experimental/immunology ; Inflammation/metabolism ; Mice ; Microglia/cytology ; Microglia/metabolism ; Multiple Sclerosis/metabolism ; Oligodendroglia/cytology ; Oligodendroglia/metabolism ; Spinal Cord/cytology ; Spinal Cord/metabolism
    Language English
    Publishing date 2021-03-15
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells10030651
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book ; Online: CiRA

    Fischbach, Jannik / Frattini, Julian / Vogelsang, Andreas

    A Tool for the Automatic Detection of Causal Relationships in Requirements Artifacts

    2021  

    Abstract: Requirements often specify the expected system behavior by using causal relations (e.g ... If A, then B). Automatically extracting these relations supports, among others, two prominent RE use cases ...

    Abstract Requirements often specify the expected system behavior by using causal relations (e.g., If A, then B). Automatically extracting these relations supports, among others, two prominent RE use cases: automatic test case derivation and dependency detection between requirements. However, existing tools fail to extract causality from natural language with reasonable performance. In this paper, we present our tool CiRA (Causality detection in Requirements Artifacts), which represents a first step towards automatic causality extraction from requirements. We evaluate CiRA on a publicly available data set of 61 acceptance criteria (causal: 32; non-causal: 29) describing the functionality of the German Corona-Warn-App. We achieve a macro F_1 score of 83%, which corroborates the feasibility of our approach.
    Keywords Computer Science - Software Engineering
    Publishing date 2021-03-11
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: The impact of previous health on the mortality after aneurysmal subarachnoid hemorrhage: analysis of a prospective Swedish multicenter study.

    Ronne Engström, Elisabeth / Baldvinsdóttir, Bryndís / Aineskog, Helena / Alpkvist, Peter / Enblad, Per / Eneling, Johanna / Fridriksson, Steen / Hillman, Jan / Klurfan, Paula / Kronvall, Erik / Lindvall, Peter / Von Vogelsang, Ann-Christin / Nilsson, Ola G / Svensson, Mikael

    Acta neurochirurgica

    2023  Volume 165, Issue 2, Page(s) 443–449

    Abstract: Purpose: There is an an increasing awareness of the importance of health and lifestyle for stroke diseases like spontaneous subarachnoid hemorrhage (SAH). However, the importance of pre-existing medical conditions for clinical course and mortality after ...

    Abstract Purpose: There is an an increasing awareness of the importance of health and lifestyle for stroke diseases like spontaneous subarachnoid hemorrhage (SAH). However, the importance of pre-existing medical conditions for clinical course and mortality after SAH has not been studied. The aim of the present study was to identify pre-existing conditions contributing to mortality after SAH.
    Methods: Data were extracted from a Swedish national prospective study on patients with SAH. Variables were defined for age, sex, body mass index (BMI), clinical condition at admission, and for 10 pre-existing medical conditions. Models predicting mortality in three time intervals with all possible subsets of these variables were generated, compared and selected using Akaike's information criterion.
    Results: 1155 patients with ruptured aneurysms were included. The mortality within 1 week was 7.6%, 1 month 14.3%, and 1 year 18.7%. The most common pre-existing medical conditions were smoking (57.6%) and hypertension (38.7%). The model's best predicting mortality within 1 week and from 1 week to 1 month included only the level of consciousness at admission and age, and these two variables were present in all the models among the top 200 in Akaike score for each time period. The most predictive model for mortality between 1 month and 1 year added previous stroke, diabetes, psychiatric disease, and BMI as predictors.
    Conclusion: Mortality within the first month was best predicted simply by initial level of consciousness and age, while mortality within from 1 month to 1 year was significantly influenced by pre-existing medical conditions.
    MeSH term(s) Humans ; Subarachnoid Hemorrhage ; Prospective Studies ; Intracranial Aneurysm ; Sweden/epidemiology ; Stroke
    Language English
    Publishing date 2023-01-12
    Publishing country Austria
    Document type Multicenter Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80010-7
    ISSN 0942-0940 ; 0001-6268
    ISSN (online) 0942-0940
    ISSN 0001-6268
    DOI 10.1007/s00701-022-05464-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: One Brain—All Cells

    Christina B. Schroeter / Alexander M. Herrmann / Stefanie Bock / Anna Vogelsang / Susann Eichler / Philipp Albrecht / Sven G. Meuth / Tobias Ruck

    Cells, Vol 10, Iss 651, p

    A Comprehensive Protocol to Isolate All Principal CNS-Resident Cell Types from Brain and Spinal Cord of Adult Healthy and EAE Mice

    2021  Volume 651

    Abstract: In experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, the role of each central nervous system (CNS)-resident cell type during inflammation, neurodegeneration, and remission has been frequently addressed. Although ... ...

    Abstract In experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, the role of each central nervous system (CNS)-resident cell type during inflammation, neurodegeneration, and remission has been frequently addressed. Although protocols for the isolation of different individual CNS-resident cell types exist, none can harvest all of them within a single experiment. In addition, isolation of individual cells is more demanding in adult mice and even more so from the inflamed CNS. Here, we present a protocol for the simultaneous purification of viable single-cell suspensions of all principal CNS-resident cell types (microglia, oligodendrocytes, astrocytes, and neurons) from adult mice—applicable in healthy mice as well as in EAE. After dissociation of the brain and spinal cord from adult mice, microglia, oligodendrocytes, astrocytes and, neurons were isolated via magnetic-activated cell sorting (MACS). Validations comprised flow cytometry, immunocytochemistry, as well as functional analyses (immunoassay and Sholl analysis). The purity of each cell isolation averaged 90%. All cells displayed cell-type-specific morphologies and expressed specific surface markers. In conclusion, this new protocol for the simultaneous isolation of all major CNS-resident cell types from one CNS offers a sophisticated and comprehensive way to investigate complex cellular networks ex vivo and simultaneously reduce mice numbers to be sacrificed.
    Keywords single-cell isolation ; demyelinating autoimmune diseases ; astrocytes ; microglia ; neurons ; oligodendrocytes ; Biology (General) ; QH301-705.5
    Subject code 572
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Development of a deep learning model for the histologic diagnosis of dysplasia in Barrett's esophagus.

    Faghani, Shahriar / Codipilly, D Chamil / David Vogelsang / Moassefi, Mana / Rouzrokh, Pouria / Khosravi, Bardia / Agarwal, Siddharth / Dhaliwal, Lovekirat / Katzka, David A / Hagen, Catherine / Lewis, Jason / Leggett, Cadman L / Erickson, Bradley J / Iyer, Prasad G

    Gastrointestinal endoscopy

    2022  Volume 96, Issue 6, Page(s) 918–925.e3

    Abstract: Background and aims: The risk of progression in Barrett's esophagus (BE) increases with development of dysplasia. There is a critical need to improve the diagnosis of BE dysplasia, given substantial interobserver disagreement among expert pathologists ... ...

    Abstract Background and aims: The risk of progression in Barrett's esophagus (BE) increases with development of dysplasia. There is a critical need to improve the diagnosis of BE dysplasia, given substantial interobserver disagreement among expert pathologists and overdiagnosis of dysplasia by community pathologists. We developed a deep learning model to predict dysplasia grade on whole-slide imaging.
    Methods: We digitized nondysplastic BE (NDBE), low-grade dysplasia (LGD), and high-grade dysplasia (HGD) histology slides. Two expert pathologists confirmed all histology and digitally annotated areas of dysplasia. Training, validation, and test sets were created (by a random 70/20/10 split). We used an ensemble approach combining a "you only look once" model to identify regions of interest and histology class (NDBE, LGD, or HGD) followed by a ResNet101 model pretrained on ImageNet applied to the regions of interest. Diagnostic performance was determined for the whole slide.
    Results: We included slides from 542 patients (164 NDBE, 226 LGD, and 152 HGD) yielding 8596 bounding boxes in the training set, 1946 bounding boxes in the validation set, and 840 boxes in the test set. When the ensemble model was used, sensitivity and specificity for LGD was 81.3% and 100%, respectively, and >90% for NDBE and HGD. The overall positive predictive value and sensitivity metric (calculated as F1 score) was .91 for NDBE, .90 for LGD, and 1.0 for HGD.
    Conclusions: We successfully trained and validated a deep learning model to accurately identify dysplasia on whole-slide images. This model can potentially help improve the histologic diagnosis of BE dysplasia and the appropriate application of endoscopic therapy.
    MeSH term(s) Humans ; Barrett Esophagus/diagnosis ; Barrett Esophagus/pathology ; Deep Learning ; Esophageal Neoplasms/pathology ; Adenocarcinoma/pathology ; Disease Progression ; Hyperplasia
    Language English
    Publishing date 2022-06-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 391583-9
    ISSN 1097-6779 ; 0016-5107
    ISSN (online) 1097-6779
    ISSN 0016-5107
    DOI 10.1016/j.gie.2022.06.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: How I treat chronic graft-versus-host disease.

    Vogelsang, G B

    Blood

    2001  Volume 97, Issue 5, Page(s) 1196–1201

    Abstract: Allogeneic stem cell transplantation (SCT) is now a commonplace procedure. Clinicians who care for patients with hematologic malignancies and aplastic anemia are almost certain to follow up patients after SCT. This review is intended to help clinicians ... ...

    Abstract Allogeneic stem cell transplantation (SCT) is now a commonplace procedure. Clinicians who care for patients with hematologic malignancies and aplastic anemia are almost certain to follow up patients after SCT. This review is intended to help clinicians observe patients for probably the most important late complication of SCT, chronic graft-versus-host disease (GVHD). It reviews the pathophysiology, risk factors, clinical manifestations, evaluation, treatment, and supportive care of chronic GVHD.
    MeSH term(s) Animals ; Chronic Disease ; Graft vs Host Disease/diagnosis ; Graft vs Host Disease/etiology ; Graft vs Host Disease/therapy ; Hematopoietic Stem Cell Transplantation/adverse effects ; Humans ; Risk Factors
    Language English
    Publishing date 2001-03-01
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 80069-7
    ISSN 1528-0020 ; 0006-4971
    ISSN (online) 1528-0020
    ISSN 0006-4971
    DOI 10.1182/blood.v97.5.1196
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Advances in the treatment of graft-versus-host disease.

    Vogelsang, G B

    Leukemia

    2000  Volume 14, Issue 3, Page(s) 509–510

    Abstract: Graft-versus-host disease (GVHD) is a complicated disease whose treatment requires an equally multifaceted approach. Recipient conditioning, donor T cell activation, and end stage effectors all may be potential targets for treatment. Many drugs used in ... ...

    Abstract Graft-versus-host disease (GVHD) is a complicated disease whose treatment requires an equally multifaceted approach. Recipient conditioning, donor T cell activation, and end stage effectors all may be potential targets for treatment. Many drugs used in the past are returning to the forefront for investigation. Some of the newer nucleoside analogs that are in various stages of development, such as fludarabine and pentostatin, are showing promising activity in GVHD.
    MeSH term(s) Antibodies, Monoclonal/therapeutic use ; Clinical Trials, Phase I as Topic ; Cytokines/physiology ; Graft vs Host Disease/prevention & control ; Graft vs Host Disease/therapy ; Humans ; Immunosuppressive Agents/therapeutic use ; Interleukin-11/pharmacology ; Interleukin-11/therapeutic use ; Intestines/drug effects ; Intestines/radiation effects ; Leukocyte Transfusion ; Lymphocyte Activation/drug effects ; Radiation-Protective Agents/pharmacology ; Radiation-Protective Agents/therapeutic use ; Sirolimus/therapeutic use ; T-Lymphocytes, Cytotoxic/immunology ; Th1 Cells/drug effects ; Th2 Cells/drug effects ; Tissue Donors ; Transplantation Conditioning/adverse effects ; Tumor Necrosis Factor-alpha/antagonists & inhibitors ; Tumor Necrosis Factor-alpha/physiology
    Chemical Substances Antibodies, Monoclonal ; Cytokines ; Immunosuppressive Agents ; Interleukin-11 ; Radiation-Protective Agents ; Tumor Necrosis Factor-alpha ; Sirolimus (W36ZG6FT64)
    Language English
    Publishing date 2000-03
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 807030-1
    ISSN 1476-5551 ; 0887-6924
    ISSN (online) 1476-5551
    ISSN 0887-6924
    DOI 10.1038/sj.leu.2401687
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Book ; Online: How Do Practitioners Interpret Conditionals in Requirements?

    Fischbach, Jannik / Frattini, Julian / Mendez, Daniel / Unterkalmsteiner, Michael / Femmer, Henning / Vogelsang, Andreas

    2021  

    Abstract: Context: Conditional statements like "If A and B then C" are core elements for describing software ... to interpret them formally. This affects any analysis that builds upon formalized requirements (e.g ...

    Abstract Context: Conditional statements like "If A and B then C" are core elements for describing software requirements. However, there are many ways to express such conditionals in natural language and also many ways how they can be interpreted. We hypothesize that conditional statements in requirements are a source of ambiguity, potentially affecting downstream activities such as test case generation negatively. Objective: Our goal is to understand how specific conditionals are interpreted by readers who work with requirements. Method: We conduct a descriptive survey with 104 RE practitioners and ask how they interpret 12 different conditional clauses. We map their interpretations to logical formulas written in Propositional (Temporal) Logic and discuss the implications. Results: The conditionals in our tested requirements were interpreted ambiguously. We found that practitioners disagree on whether an antecedent is only sufficient or also necessary for the consequent. Interestingly, the disagreement persists even when the system behavior is known to the practitioners. We also found that certain cue phrases are associated with specific interpretations. Conclusion: Conditionals in requirements are a source of ambiguity and there is not just one way to interpret them formally. This affects any analysis that builds upon formalized requirements (e.g., inconsistency checking, test-case generation). Our results may also influence guidelines for writing requirements.
    Keywords Computer Science - Software Engineering
    Subject code 160
    Publishing date 2021-09-05
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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