Article ; Online: No effect of NGAL/lipocalin-2 on aggressiveness of cancer in the MMTV-PyMT/FVB/N mouse model for breast cancer.
PloS one
2012 Volume 7, Issue 6, Page(s) e39646
Abstract: ... mammary tumor virus-polyoma middle T antigen (MMTV-PyMT) mice were crossed into the same FVB/N background ...
| Abstract | NGAL/lipocalin-2 is a siderophore-binding protein that is highly expressed in several cancers. It is suggested to confer a proliferative advantage to cancer cells. Its expression has been correlated with aggressiveness of breast cancer as determined both in patients and in mouse breast cancer models. This was recently confirmed in two mouse models of spontaneous breast cancer in wild-type and lipocalin-2-deficient mice. We used a similar strategy using a different mouse strain. Lipocalin-2-deficient mice and mouse mammary tumor virus-polyoma middle T antigen (MMTV-PyMT) mice were crossed into the same FVB/N background. All mice developed tumors by week 8. The mice were sacrificed on week 13 and tissue was processed for biochemical and histological analysis. The total tumor volume and number of metastases were quantitated in 26 lipocalin-2-deficient mice and 34 wild-type controls. Lipocalin-2 expression in tumors of MMTV-PyMT-positive and wild-type mice was assessed by quantitative real-time PCR and by immunohistochemistry. The expression of the lipocalin-2 receptors 24p3R and megalin and of Mmp-9, transferrin receptor, and Bdh2 (a producer of a mammalian siderophore) were quantitated by real-time PCR. No significant difference was observed between wild-type and lipocalin-2-deficient mice. Lipocalin-2 was highly expressed in tumors from wild-type mice, but the expression did not correlate with tumor size. No effect of lipocalin-2 was observed with respect to time to tumor appearance, total tumor volume, or to the number of metastases. Histology and gelatinolytic activity of the mammary tumors did not differ between wild-type and lipocalin-2-deficient mice. We conclude that NGAL/lipocalin-2 does not invariably affect the aggressiveness of breast cancers as assessed in mouse models, thus questioning the role of lipocalin-2 in cancer development. |
|---|---|
| MeSH term(s) | Acute-Phase Proteins/metabolism ; Animals ; Antigens, Polyomavirus Transforming/metabolism ; Cell Line, Tumor ; Disease Models, Animal ; Female ; Gene Expression Regulation, Neoplastic ; Granulocytes/cytology ; Humans ; Immunohistochemistry/methods ; Lipocalin-2 ; Lipocalins/metabolism ; Low Density Lipoprotein Receptor-Related Protein-2/biosynthesis ; Male ; Matrix Metalloproteinase 9/biosynthesis ; Mice ; Mice, Knockout ; Oncogene Proteins/metabolism ; Receptors, Cell Surface/biosynthesis ; Receptors, Transferrin/biosynthesis |
| Chemical Substances | 24p3 receptor, mouse ; Acute-Phase Proteins ; Antigens, Polyomavirus Transforming ; Lipocalin-2 ; Lipocalins ; Low Density Lipoprotein Receptor-Related Protein-2 ; Oncogene Proteins ; Receptors, Cell Surface ; Receptors, Transferrin ; Lcn2 protein, mouse (126469-30-5) ; Matrix Metalloproteinase 9 (EC 3.4.24.35) |
| Language | English |
| Publishing date | 2012-06-21 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ISSN | 1932-6203 |
| ISSN (online) | 1932-6203 |
| DOI | 10.1371/journal.pone.0039646 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.