LIVIVO - Search results -

Search results

Result 1 - 10 of total 68

Search options

Article ; Online: Corrigendum: Detection of Apoptotic Cells Using Propidium Iodide Staining.

Newbold, Andrea / Martin, Ben P / Cullinane, Carleen / Bots, Michael

Cold Spring Harbor protocols

2018 Volume 2018, Issue 6, Page(s) Corr104943

Language	English
Publishing date	2018-06-01
Publishing country	United States
Document type	Journal Article ; Published Erratum
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.corr104943
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Correction to: Combining the differentiating effect of panobinostat with the apoptotic effect of arsenic trioxide leads to significant survival benefit in a model of t(8;21) acute myeloid leukemia.

Salmon, Jessica M / Bots, Michael / Vidacs, Eva / Stanley, Kym L / Atadja, Peter / Zuber, Johannes / Johnstone, Ricky W

Clinical epigenetics

2020 Volume 12, Issue 1, Page(s) 178

Abstract: An amendment to this paper has been published and can be accessed via the original article. ...

Abstract	An amendment to this paper has been published and can be accessed via the original article.
Language	English
Publishing date	2020-11-18
Publishing country	Germany
Document type	Published Erratum
ZDB-ID	2553921-8
ISSN	1868-7083 ; 1868-7075
ISSN (online)	1868-7083
ISSN	1868-7075
DOI	10.1186/s13148-020-00964-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Rational combinations using HDAC inhibitors.

Bots, Michael / Johnstone, Ricky W

Clinical cancer research : an official journal of the American Association for Cancer Research

2009 Volume 15, Issue 12, Page(s) 3970–3977

Abstract: In addition to well-characterized genetic abnormalities that lead to cancer onset and progression, it is now recognized that alterations to the epigenome may also play a significant role in oncogenesis. As a result, epigenetic-modulating agents such as ... ...

Abstract	In addition to well-characterized genetic abnormalities that lead to cancer onset and progression, it is now recognized that alterations to the epigenome may also play a significant role in oncogenesis. As a result, epigenetic-modulating agents such as histone deacetylase inhibitors (HDACi) have attracted enormous attention as anticancer drugs. In numerous in vitro and preclinical settings, these compounds have shown their vast potential as single agent anticancer therapies, but unfortunately equivalent responses have not always been observed in patients. Given the pleiotropic effects HDACi have on malignant cells, their true therapeutic potential most likely lies in combination with other anticancer drugs. In this review we will focus on the anticancer effects of HDACi when combined with other cancer therapeutics with an emphasis on those combinations based on a strong molecular rationale.
MeSH term(s)	Antineoplastic Agents/therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; Apoptosis Regulatory Proteins/metabolism ; Clinical Trials as Topic ; Enzyme Inhibitors/therapeutic use ; Histone Deacetylase Inhibitors ; Humans ; Myelodysplastic Syndromes/drug therapy ; Myelodysplastic Syndromes/genetics ; Neoplasms/drug therapy ; Neoplasms/genetics
Chemical Substances	Antineoplastic Agents ; Apoptosis Regulatory Proteins ; Enzyme Inhibitors ; Histone Deacetylase Inhibitors
Language	English
Publishing date	2009-06-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1225457-5
ISSN	1557-3265 ; 1078-0432
ISSN (online)	1557-3265
ISSN	1078-0432
DOI	10.1158/1078-0432.CCR-08-2786
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4223: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Serpins in T cell immunity.

Bots, Michael / Medema, Jan Paul

Journal of leukocyte biology

2008 Volume 84, Issue 5, Page(s) 1238–1247

Abstract: Serine protease inhibitors (serpins) are a family of proteins that are important in the regulation of several biological processes. This mainly involves the inhibition of serine proteases, although some serpins inhibit a different class of proteases or ... ...

Abstract	Serine protease inhibitors (serpins) are a family of proteins that are important in the regulation of several biological processes. This mainly involves the inhibition of serine proteases, although some serpins inhibit a different class of proteases or even function without inhibitory activity. In contrast to other protease inhibitor families, serpins inhibit their target proteases by a specific mechanism, which depends on a change in conformation. This review primarily focuses on one subgroup of serpins--ovalbumin (ov)-serpins. Different than most members of the family, this group of serpins lacks secretion signal sequences and therefore, mainly functions intracellularly. In addition to expression in most normal tissues, ov-serpins can be found in multiple different cells of the immune system. Interestingly, expression of ov-serpins in these cells is tightly regulated, indicating a role for these serpins in the regulation of immune responses. The role of serpins in the immune response will be the topic of this review.
MeSH term(s)	CD8-Positive T-Lymphocytes/immunology ; Humans ; Immunologic Memory ; Neoplasms/immunology ; Ovalbumin/physiology ; Serpins/immunology ; Serpins/metabolism ; T-Lymphocytes/immunology ; T-Lymphocytes/metabolism
Chemical Substances	Serpins ; Ovalbumin (9006-59-1)
Language	English
Publishing date	2008-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	605722-6
ISSN	1938-3673 ; 0741-5400
ISSN (online)	1938-3673
ISSN	0741-5400
DOI	10.1189/jlb.0208140
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 603: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Discovery of biomarkers for the presence and progression of left ventricular diastolic dysfunction and HEart faiLure with Preserved ejection Fraction in patients at risk for cardiovascular disease: rationale and design of the HELPFul case-cohort study in a Dutch cardiology outpatient clinic.

Valstar, Gideon B / Bots, Sophie H / Groepenhoff, Floor / Gohar, Aisha / Rutten, Frans H / Leiner, Tim / Cramer, Maarten Jan Maria / Teske, Arco J / Suciadi, Leonardo P / Menken, Roxana / Pasterkamp, Gerard / Asselbergs, Folkert W / Hofstra, Leonard / Bots, Michael L / den Ruijter, Hester M

BMJ open

2019 Volume 9, Issue 6, Page(s) e028408

Abstract: Introduction: Left ventricular diastolic dysfunction (LVDD) is a common condition in both sexes that may deteriorate into heart failure (HF) with preserved ejection fraction (pEF), although this seems to happen more often in women than in men. Both LVDD ...

Abstract	Introduction: Left ventricular diastolic dysfunction (LVDD) is a common condition in both sexes that may deteriorate into heart failure (HF) with preserved ejection fraction (pEF), although this seems to happen more often in women than in men. Both LVDD and HFpEF often go unrecognised, necessitating the discovery of biomarkers that aid both the identification of individuals with LVDD at risk of developing HF and identification of individuals most likely to benefit from treatment. Methods and analysis: HELPFul is an ongoing case-cohort study at a Dutch cardiology outpatient clinic enrolling patients aged 45 years and older without history of cardiovascular disease, who were referred by the general practitioner for cardiac evaluation. We included a random sample of patients and enriched the cohort with cases (defined as an E/e' ≥8 measured with echocardiography). Information about medical history, cardiovascular risk factors, electrocardiography, echocardiography, exercise test performance, common carotid intima-media thickness measurement and standard cardiovascular biomarkers was obtained from the routine care data collected by the cardiology outpatient clinic. Study procedure consists of extensive venous blood collection for biobanking and additional standardised questionnaires. Follow-up will consist of standardised questionnaires by mail and linkage to regional and national registries. We will perform cardiac magnetic resonance imaging and coronary CT angiography in a subgroup of patients to investigate the extent of macrovascular and microvascular coronary disease. Ethics and dissemination: The study protocol was approved by the Institutional Review Board of the University Medical Center Utrecht. Results will be disseminated through national and international conferences and in peer-reviewed journals in cardiovascular disease. Trial registration: NTR6016;Pre-results.
MeSH term(s)	Ambulatory Care Facilities ; Biomarkers ; Cardiology/methods ; Carotid Intima-Media Thickness/statistics & numerical data ; Cohort Studies ; Disease Progression ; Echocardiography/statistics & numerical data ; Exercise Test/statistics & numerical data ; Female ; Heart Failure/diagnosis ; Heart Failure/physiopathology ; Humans ; Male ; Middle Aged ; Netherlands ; Prospective Studies ; Risk ; Ventricular Dysfunction, Left/diagnosis ; Ventricular Dysfunction, Left/physiopathology
Chemical Substances	Biomarkers
Language	English
Publishing date	2019-06-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2599832-8
ISSN	2044-6055 ; 2044-6055
ISSN (online)	2044-6055
ISSN	2044-6055
DOI	10.1136/bmjopen-2018-028408
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Detection of apoptotic cells using immunohistochemistry.

Newbold, Andrea / Martin, Ben P / Cullinane, Carleen / Bots, Michael

Cold Spring Harbor protocols

2014 Volume 2014, Issue 11, Page(s) 1196–1201

Abstract: Immunohistochemistry is commonly used to show the presence of apoptotic cells in situ. In this protocol, B-cell lymphoma cells are injected into recipient mice and, on tumor formation, the mice are treated with the apoptosis inducer vorinostat (a histone ...

Abstract	Immunohistochemistry is commonly used to show the presence of apoptotic cells in situ. In this protocol, B-cell lymphoma cells are injected into recipient mice and, on tumor formation, the mice are treated with the apoptosis inducer vorinostat (a histone deacetylase inhibitor). Tumor samples are fixed and sectioned, and fragmented DNA (a feature of apoptotic cells) is end-labeled by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL). Immunohistochemical methods are then used to detect the labeled DNA and identify B-cell lymphoma cells in the last stage of apoptosis. Because the assay can lead to false-positive results, it is advisable to carry out an additional assay (e.g., immunohistochemistry for active caspase-3) to confirm the presence of apoptotic cells.
MeSH term(s)	Animals ; Apoptosis ; DNA/analysis ; DNA Fragmentation ; Histone Deacetylase Inhibitors/administration & dosage ; Hydroxamic Acids/administration & dosage ; Immunohistochemistry/methods ; In Situ Nick-End Labeling ; Lymphoma, B-Cell/pathology ; Mice
Chemical Substances	Histone Deacetylase Inhibitors ; Hydroxamic Acids ; vorinostat (58IFB293JI) ; DNA (9007-49-2)
Language	English
Publishing date	2014-11-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot082537
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Measuring apoptosis in mammals in vivo.

Newbold, Andrea / Martin, Ben P / Cullinane, Carleen / Bots, Michael

Cold Spring Harbor protocols

2014 Volume 2014, Issue 11, Page(s) 1125–1127

Abstract: Apoptosis is a mode of cell death that is essential in multicellular organisms for the removal of superfluous, damaged, or potentially dangerous cells during development, infection, or normal tissue homeostasis. To prevent inflammation, cells undergoing ... ...

Abstract	Apoptosis is a mode of cell death that is essential in multicellular organisms for the removal of superfluous, damaged, or potentially dangerous cells during development, infection, or normal tissue homeostasis. To prevent inflammation, cells undergoing apoptosis produce "find-me" signals that trigger the recruitment of phagocytes, which clear the apoptotic cells on recognition of "eat-me" signals. Despite the loss of billions of cells per day by apoptosis in the human body, the number of apoptotic cells found in healthy tissue is surprisingly low and reflects the efficiency of this process. However, in certain conditions (e.g., in cancer cells responding to chemotherapy), the number of apoptotic cells is too high to be efficiently cleared by phagocytes, and apoptotic cells can be observed. In these situations, the detection of apoptosis may be helpful in monitoring disease progression as well as in predicting the responses of tumors to anticancer therapies. Here we introduce various methods for monitoring apoptotic cells in vivo using a murine model of B-cell lymphoma and a solid tumor xenograft.
MeSH term(s)	Animals ; Apoptosis ; Heterografts/pathology ; Lymphoma, B-Cell/pathology ; Mice ; Neoplasm Transplantation
Language	English
Publishing date	2014-11-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.top070417
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Fluorodeoxyglucose-based positron emission tomography imaging to monitor drug responses in solid tumors.

Newbold, Andrea / Martin, Ben P / Cullinane, Carleen / Bots, Michael

Cold Spring Harbor protocols

2014 Volume 2014, Issue 10, Page(s) pdb.prot082529

Abstract: Positron emission tomography (PET) is used to monitor the uptake of the labeled glucose analogue fluorodeoxyglucose (¹⁸F-FDG) by solid tumor cells, a process generally believed to reflect viable tumor cell mass. The use of ¹⁸F-FDG exploits the high ... ...

Abstract	Positron emission tomography (PET) is used to monitor the uptake of the labeled glucose analogue fluorodeoxyglucose (¹⁸F-FDG) by solid tumor cells, a process generally believed to reflect viable tumor cell mass. The use of ¹⁸F-FDG exploits the high demand for glucose in tumor cells, and serves to document over time the response of a solid tumor to an inducer of apoptosis. The apoptosis inducer crizotinib is a small-molecule inhibitor of c-Met, a receptor tyrosine kinase that is often dysregulated in human tumors. In this protocol, we describe how to monitor the response of a solid tumor to crizotinib. Human gastric tumor cells (GTL-16 cells) are injected into recipient mice and, on tumor formation, the mice are treated with crizotinib. The tracer ¹⁸F-FDG is then injected into the mice at several time points, and its uptake is monitored using PET. Because ¹⁸F-FDG uptake varies widely among different tumor models, preliminary experiments should be performed with each new model to determine its basal level of ¹⁸F-FDG uptake. Verifying that the basal level of uptake is sufficiently above background levels will assure accurate quantitation. Because ¹⁸F-FDG uptake is not a direct measure of apoptosis, it is advisable to carry out an additional direct method to show the presence of apoptotic cells.
MeSH term(s)	Animals ; Antineoplastic Agents/therapeutic use ; Cell Line, Tumor ; Disease Models, Animal ; Fluorodeoxyglucose F18 ; Humans ; Mice ; Positron-Emission Tomography ; Pyrazoles/therapeutic use ; Pyridines/therapeutic use ; Stomach Neoplasms/diagnostic imaging ; Stomach Neoplasms/drug therapy ; Xenograft Model Antitumor Assays
Chemical Substances	Antineoplastic Agents ; Pyrazoles ; Pyridines ; Fluorodeoxyglucose F18 (0Z5B2CJX4D) ; crizotinib (53AH36668S)
Language	English
Publishing date	2014-10-01
Publishing country	United States
Document type	Journal Article
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot082529
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Fluorodeoxyglucose-based positron emission tomography imaging to monitor drug responses in hematological tumors.

Newbold, Andrea / Martin, Ben P / Cullinane, Carleen / Bots, Michael

Cold Spring Harbor protocols

2014 Volume 2014, Issue 10, Page(s) pdb.prot082511

Abstract: Positron emission tomography (PET) can be used to monitor the uptake of the labeled glucose analog fluorodeoxyglucose (¹⁸F-FDG), a process that is generally believed to reflect viable tumor cell mass. The use of ¹⁸F-FDG PET can be helpful in documenting ... ...

Abstract	Positron emission tomography (PET) can be used to monitor the uptake of the labeled glucose analog fluorodeoxyglucose (¹⁸F-FDG), a process that is generally believed to reflect viable tumor cell mass. The use of ¹⁸F-FDG PET can be helpful in documenting over time the reduction in tumor mass volume in response to anticancer drug therapy in vivo. In this protocol, we describe how to monitor the response of murine B-cell lymphomas to an inducer of apoptosis, the anticancer drug vorinostat (a histone deacetylase inhibitor). B-cell lymphoma cells are injected into recipient mice and, on tumor formation, the mice are treated with vorinostat. The tracer ¹⁸F-FDG is then injected into the mice at several time points, and its uptake is monitored using PET. Because the uptake of ¹⁸F-FDG is not a direct measure of apoptosis, an additional direct method proving that apoptotic cells are present should also be performed.
MeSH term(s)	Animals ; Antineoplastic Agents/therapeutic use ; Cell Line, Tumor ; Fluorodeoxyglucose F18 ; Humans ; Hydroxamic Acids/therapeutic use ; Lymphoma, B-Cell/diagnostic imaging ; Lymphoma, B-Cell/drug therapy ; Mice ; Mice, Inbred C57BL ; Positron-Emission Tomography ; Xenograft Model Antitumor Assays
Chemical Substances	Antineoplastic Agents ; Hydroxamic Acids ; Fluorodeoxyglucose F18 (0Z5B2CJX4D) ; vorinostat (58IFB293JI)
Language	English
Publishing date	2014-10-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot082511
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Detection of apoptotic cells using propidium iodide staining.

Newbold, Andrea / Martin, Ben P / Cullinane, Carleen / Bots, Michael

Cold Spring Harbor protocols

2014 Volume 2014, Issue 11, Page(s) 1202–1206

Abstract: Flow cytometry assays are often used to detect apoptotic cells in in vitro cultures. Depending on the experimental model, these assays can also be useful in evaluating apoptosis in vivo. In this protocol, we describe a propidium iodide (PI) flow ... ...

Abstract	Flow cytometry assays are often used to detect apoptotic cells in in vitro cultures. Depending on the experimental model, these assays can also be useful in evaluating apoptosis in vivo. In this protocol, we describe a propidium iodide (PI) flow cytometry assay to evaluate B-cell lymphomas that have undergone apoptosis in vivo. B-cell lymphoma cells are injected into recipient mice and, on tumor formation, the mice are treated with the apoptosis inducer vorinostat (a histone deacetylase inhibitor). Tumor samples collected from the lymph nodes and/or the spleen are used to prepare a single-cell suspension that is exposed to a hypotonic solution containing the fluorochrome PI. The DNA content of the cells, now labeled with PI, is analyzed by flow cytometry. Nuclear DNA content is lost during apoptosis, resulting in a hypodiploid (or sub-G1) DNA profile during flow cytometry. In contrast, healthy cells display a sharp diploid DNA profile.
MeSH term(s)	Animals ; Apoptosis ; DNA/analysis ; Flow Cytometry/methods ; Histone Deacetylase Inhibitors/administration & dosage ; Hydroxamic Acids/administration & dosage ; Lymphoma, B-Cell/pathology ; Mice ; Propidium/metabolism ; Staining and Labeling/methods
Chemical Substances	Histone Deacetylase Inhibitors ; Hydroxamic Acids ; Propidium (36015-30-2) ; vorinostat (58IFB293JI) ; DNA (9007-49-2)
Language	English
Publishing date	2014-11-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot082545
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED