LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 162

Search options

  1. Article ; Online: Efficient knock-in method enabling lineage tracing in zebrafish.

    Mi, Jiarui / Andersson, Olov

    Life science alliance

    2023  Volume 6, Issue 5

    Abstract: Here, we devised a cloning-free 3' knock-in strategy for zebrafish using PCR amplified dsDNA donors that avoids disrupting the targeted genes. The dsDNA donors carry genetic cassettes coding for fluorescent proteins and Cre recombinase in frame with the ... ...

    Abstract Here, we devised a cloning-free 3' knock-in strategy for zebrafish using PCR amplified dsDNA donors that avoids disrupting the targeted genes. The dsDNA donors carry genetic cassettes coding for fluorescent proteins and Cre recombinase in frame with the endogenous gene but separated from it by self-cleavable peptides. Primers with 5' AmC6 end-protections generated PCR amplicons with increased integration efficiency that were coinjected with preassembled Cas9/gRNA ribonucleoprotein complexes for early integration. We targeted four genetic loci (
    MeSH term(s) Animals ; Zebrafish/genetics ; Liver ; DNA Primers ; Genetic Loci ; Hematopoietic Stem Cells
    Chemical Substances DNA Primers
    Language English
    Publishing date 2023-03-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2575-1077
    ISSN (online) 2575-1077
    DOI 10.26508/lsa.202301944
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: Decoding pancreatic endocrine cell differentiation and β cell regeneration in zebrafish.

    Mi, Jiarui / Liu, Ka-Cheuk / Andersson, Olov

    Science advances

    2023  Volume 9, Issue 33, Page(s) eadf5142

    Abstract: In contrast to mice, zebrafish have an exceptional yet elusive ability to replenish lost β cells in adulthood. Understanding this framework would provide mechanistic insights for β cell regeneration, which may be extrapolated to humans. Here, we ... ...

    Abstract In contrast to mice, zebrafish have an exceptional yet elusive ability to replenish lost β cells in adulthood. Understanding this framework would provide mechanistic insights for β cell regeneration, which may be extrapolated to humans. Here, we characterize a
    MeSH term(s) Humans ; Animals ; Mice ; Zebrafish ; Endocrine Cells ; Insulin-Secreting Cells ; Cell Differentiation ; Regeneration
    Language English
    Publishing date 2023-08-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.adf5142
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Book ; Online: Material-agnostic Shaping of Granular Materials with Optimal Transport

    Alatur, Nikhilesh / Andersson, Olov / Siegwart, Roland / Ott, Lionel

    2023  

    Abstract: From construction materials, such as sand or asphalt, to kitchen ingredients, like rice, sugar, or salt; the world is full of granular materials. Despite impressive progress in robotic manipulation, manipulating and interacting with granular material ... ...

    Abstract From construction materials, such as sand or asphalt, to kitchen ingredients, like rice, sugar, or salt; the world is full of granular materials. Despite impressive progress in robotic manipulation, manipulating and interacting with granular material remains a challenge due to difficulties in perceiving, representing, modelling, and planning for these variable materials that have complex internal dynamics. While some prior work has looked into estimating or learning accurate dynamics models for granular materials, the literature is still missing a more abstract planning method that can be used for planning manipulation actions for granular materials with unknown material properties. In this work, we leverage tools from optimal transport and connect them to robot motion planning. We propose a heuristics-based sweep planner that does not require knowledge of the material's properties and directly uses a height map representation to generate promising sweeps. These sweeps transform granular material from arbitrary start shapes into arbitrary target shapes. We apply the sweep planner in a fast and reactive feedback loop and avoid the need for model-based planning over multiple time steps. We validate our approach with a large set of simulation and hardware experiments where we show that our method is capable of efficiently solving several complex tasks, including gathering, separating, and shaping of several types of granular materials into different target shapes.
    Keywords Computer Science - Robotics
    Subject code 629
    Publishing date 2023-03-29
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: Role of adenosine signalling and metabolism in β-cell regeneration.

    Andersson, Olov

    Experimental cell research

    2014  Volume 321, Issue 1, Page(s) 3–10

    Abstract: Glucose homeostasis, which is controlled by the endocrine cells of the pancreas, is disrupted in both type I and type II diabetes. Deficiency in the number of insulin-producing β cells - a primary cause of type I diabetes and a secondary contributor of ... ...

    Abstract Glucose homeostasis, which is controlled by the endocrine cells of the pancreas, is disrupted in both type I and type II diabetes. Deficiency in the number of insulin-producing β cells - a primary cause of type I diabetes and a secondary contributor of type II diabetes - leads to hyperglycemia and hence an increase in the need for insulin. Although diabetes can be controlled with insulin injections, a curative approach is needed. A potential approach to curing diabetes involves regenerating the β-cell mass, e.g. by increasing β-cell proliferation, survival, neogenesis or transdifferentiation. The nucleoside adenosine and its cognate nucleotide ATP have long been known to affect insulin secretion, but have more recently been shown to increase β-cell proliferation during homeostatic control and regeneration of the β-cell mass. Adenosine is also known to have anti-inflammatory properties, and agonism of adenosine receptors can promote the survival of β-cells in an inflammatory microenvironment. In this review, both intracellular and extracellular mechanisms of adenosine and ATP are discussed in terms of their established and putative effects on β-cell regeneration.
    MeSH term(s) Adenosine/metabolism ; Animals ; Diabetes Mellitus/metabolism ; Diabetes Mellitus/pathology ; Humans ; Insulin-Secreting Cells/cytology ; Insulin-Secreting Cells/metabolism ; Regeneration/physiology ; Signal Transduction
    Chemical Substances Adenosine (K72T3FS567)
    Language English
    Publishing date 2014-02-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1493-x
    ISSN 1090-2422 ; 0014-4827
    ISSN (online) 1090-2422
    ISSN 0014-4827
    DOI 10.1016/j.yexcr.2013.11.019
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 563: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Book ; Online: COIN-LIO

    Pfreundschuh, Patrick / Oleynikova, Helen / Cadena, Cesar / Siegwart, Roland / Andersson, Olov

    Complementary Intensity-Augmented LiDAR Inertial Odometry

    2023  

    Abstract: We present COIN-LIO, a LiDAR Inertial Odometry pipeline that tightly couples information from LiDAR intensity with geometry-based point cloud registration. The focus of our work is to improve the robustness of LiDAR-inertial odometry in geometrically ... ...

    Abstract We present COIN-LIO, a LiDAR Inertial Odometry pipeline that tightly couples information from LiDAR intensity with geometry-based point cloud registration. The focus of our work is to improve the robustness of LiDAR-inertial odometry in geometrically degenerate scenarios, like tunnels or flat fields. We project LiDAR intensity returns into an intensity image, and propose an image processing pipeline that produces filtered images with improved brightness consistency within the image as well as across different scenes. To effectively leverage intensity as an additional modality, we present a novel feature selection scheme that detects uninformative directions in the point cloud registration and explicitly selects patches with complementary image information. Photometric error minimization in the image patches is then fused with inertial measurements and point-to-plane registration in an iterated Extended Kalman Filter. The proposed approach improves accuracy and robustness on a public dataset. We additionally publish a new dataset, that captures five real-world environments in challenging, geometrically degenerate scenes. By using the additional photometric information, our approach shows drastically improved robustness against geometric degeneracy in environments where all compared baseline approaches fail.
    Keywords Computer Science - Robotics
    Subject code 006
    Publishing date 2023-10-02
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Book ; Online: Learning to Fly Omnidirectional Micro Aerial Vehicles with an End-To-End Control Network

    Cuniato, Eugenio / Andersson, Olov / Oleynikova, Helen / Siegwart, Roland / Pantic, Michael

    2023  

    Abstract: Overactuated tilt-rotor platforms offer many advantages over traditional fixed-arm drones, allowing the decoupling of the applied force from the attitude of the robot. This expands their application areas to aerial interaction and manipulation, and ... ...

    Abstract Overactuated tilt-rotor platforms offer many advantages over traditional fixed-arm drones, allowing the decoupling of the applied force from the attitude of the robot. This expands their application areas to aerial interaction and manipulation, and allows them to overcome disturbances such as from ground or wall effects by exploiting the additional degrees of freedom available to their controllers. However, the overactuation also complicates the control problem, especially if the motors that tilt the arms have slower dynamics than those spinning the propellers. Instead of building a complex model-based controller that takes all of these subtleties into account, we attempt to learn an end-to-end pose controller using reinforcement learning, and show its superior behavior in the presence of inertial and force disturbances compared to a state-of-the-art traditional controller.

    Comment: Accepted and presented at the 18th International Symposium on Experimental Robotics (ISER 2023)
    Keywords Computer Science - Robotics
    Subject code 629
    Publishing date 2023-12-08
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Book ; Online: Dynablox

    Schmid, Lukas / Andersson, Olov / Sulser, Aurelio / Pfreundschuh, Patrick / Siegwart, Roland

    Real-time Detection of Diverse Dynamic Objects in Complex Environments

    2023  

    Abstract: Real-time detection of moving objects is an essential capability for robots acting autonomously in dynamic environments. We thus propose Dynablox, a novel online mapping-based approach for robust moving object detection in complex environments. The ... ...

    Abstract Real-time detection of moving objects is an essential capability for robots acting autonomously in dynamic environments. We thus propose Dynablox, a novel online mapping-based approach for robust moving object detection in complex environments. The central idea of our approach is to incrementally estimate high confidence free-space areas by modeling and accounting for sensing, state estimation, and mapping limitations during online robot operation. The spatio-temporally conservative free space estimate enables robust detection of moving objects without making any assumptions on the appearance of objects or environments. This allows deployment in complex scenes such as multi-storied buildings or staircases, and for diverse moving objects such as people carrying various items, doors swinging or even balls rolling around. We thoroughly evaluate our approach on real-world data sets, achieving 86% IoU at 17 FPS in typical robotic settings. The method outperforms a recent appearance-based classifier and approaches the performance of offline methods. We demonstrate its generality on a novel data set with rare moving objects in complex environments. We make our efficient implementation and the novel data set available as open-source.

    Comment: Code released at https://github.com/ethz-asl/dynablox
    Keywords Computer Science - Robotics
    Publishing date 2023-04-19
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article ; Online: Immunological differences between heart- and kidney-transplanted children: a cross-sectional study - CORRIGENDUM.

    Ekman-Joelsson, Britt-Marie / Brandström, Per / Allén, Maria / Andersson, Bengt / Wåhlander, Håkan / Mellgren, Karin / Ekwall, Olov

    Cardiology in the young

    2022  Volume 33, Issue 5, Page(s) 842

    MeSH term(s) Humans ; Child ; Cross-Sectional Studies ; Kidney Transplantation ; Graft Rejection ; Heart ; Kidney
    Language English
    Publishing date 2022-07-22
    Publishing country England
    Document type Journal Article ; Published Erratum
    ZDB-ID 1078466-4
    ISSN 1467-1107 ; 1047-9511
    ISSN (online) 1467-1107
    ISSN 1047-9511
    DOI 10.1017/S1047951122002347
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4050: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Book: Papers dedicated to Lennart Andersson

    Andersson, Lennart / Hedlund, Per Olov

    (Scandinavian journal of urology and nephrology : Supplementum ; 138)

    1991  

    Title variant Lennart Andersson
    Author's details ed.: Per Olov Hedlund
    Series title Scandinavian journal of urology and nephrology : Supplementum ; 138
    Scandinavian journal of urology and nephrology
    Scandinavian journal of urology and nephrology ; Supplementum
    Collection Scandinavian journal of urology and nephrology
    Scandinavian journal of urology and nephrology ; Supplementum
    Keywords Urology / essays ; Harninkontinenz ; Kind ; Urogenitalkrankheit
    Subject Blaseninkontinenz ; Incontinentia urinae ; Blasenüberaktivität ; Overactive bladder ; Überaktive Blase ; Blasenschwäche ; Urogenitalsystem ; Kindheit ; Kindesalter ; Kindschaft ; Kinder
    Language English
    Size 264 S. : Ill., graph. Darst.
    Publisher Almqvist & Wiksell
    Publishing place Stockholm
    Publishing country Sweden
    Document type Book
    HBZ-ID HT003976718
    ISBN 91-7626-099-2 ; 978-91-7626-099-9
    Database Catalogue ZB MED Medicine, Health

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 601 -Suppl.138- verfügbar in Königswinter Königswinter

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Adjudin improves beta cell maturation, hepatic glucose uptake and glucose homeostasis.

    Ren, Lipeng / Charbord, Jérémie / Chu, Lianhe / Kemas, Aurino M / Bertuzzi, Maria / Mi, Jiarui / Xing, Chen / Lauschke, Volker M / Andersson, Olov

    Diabetologia

    2023  Volume 67, Issue 1, Page(s) 137–155

    Abstract: Aims/hypothesis: Recovering functional beta cell mass is a promising approach for future diabetes therapies. The aim of the present study is to investigate the effects of adjudin, a small molecule identified in a beta cell screen using zebrafish, on ... ...

    Abstract Aims/hypothesis: Recovering functional beta cell mass is a promising approach for future diabetes therapies. The aim of the present study is to investigate the effects of adjudin, a small molecule identified in a beta cell screen using zebrafish, on pancreatic beta cells and diabetes conditions in mice and human spheroids.
    Methods: In zebrafish, insulin expression was examined by bioluminescence and quantitative real-time PCR (qPCR), glucose levels were examined by direct measurements and distribution using a fluorescent glucose analogue, and calcium activity in beta cells was analysed by in vivo live imaging. Pancreatic islets of wild-type postnatal day 0 (P0) and 3-month-old (adult) mice, as well as adult db/db mice (i.e. BKS(D)-Lepr
    Results: Adjudin treatment increased insulin expression and calcium response to glucose in beta cells and decreased glucose levels after beta cell ablation in zebrafish. Adjudin led to improved beta cell function, decreased beta cell proliferation and glucose responsive insulin secretion by decreasing basal insulin secretion in in vitro cultured newborn mouse islets. RNA-seq of P0 islets indicated that adjudin treatment resulted in increased glucose metabolism and mitochondrial function, as well as downstream signalling pathways involved in insulin secretion. In islets from db/db mice cultured in vitro, adjudin treatment strengthened beta cell identity and insulin secretion. RNA-seq of db/db islets indicated adjudin-upregulated genes associated with insulin secretion, membrane ion channel activity and exocytosis. Moreover, adjudin promoted glucose uptake in the liver of zebrafish in an insulin-independent manner, and similarly promoted glucose consumption in primary human hepatocyte spheroids with insulin resistance. In vivo studies using db/db mice revealed reduced nonfasting blood glucose, improved glucose tolerance and strengthened beta cell identity after adjudin treatment.
    Conclusions/interpretation: Adjudin promoted functional maturation of immature islets, improved function of dysfunctional islets, stimulated glucose uptake in liver and improved glucose homeostasis in db/db mice. Thus, the multifunctional drug adjudin, previously studied in various contexts and conditions, also shows promise in the management of diabetic states.
    Data availability: Raw and processed RNA-seq data for this study have been deposited in the Gene Expression Omnibus under accession number GSE235398 ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE235398 ).
    MeSH term(s) Mice ; Humans ; Animals ; Infant, Newborn ; Zebrafish ; Diabetes Mellitus, Type 2/metabolism ; Calcium/metabolism ; Islets of Langerhans/metabolism ; Glucose/metabolism ; Insulin/metabolism ; Insulin-Secreting Cells/metabolism ; Homeostasis ; Liver/metabolism
    Chemical Substances 1-(2,4-dichlorobenzyl)indazole-3-carbohydrazide ; Calcium (SY7Q814VUP) ; Glucose (IY9XDZ35W2) ; Insulin
    Language English
    Publishing date 2023-10-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1694-9
    ISSN 1432-0428 ; 0012-186X
    ISSN (online) 1432-0428
    ISSN 0012-186X
    DOI 10.1007/s00125-023-06020-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 279: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top