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  1. Article: Novel Compounds Derived from DFPM Induce Root Growth Arrest through the Specific

    Kim, Seojung / Cho, Miri / Kim, Tae-Houn

    Life (Basel, Switzerland)

    2023  Volume 13, Issue 9

    Abstract: The small compound [5-(3,4-dichlorophenyl) furan-2-yl]-piperidine-1-ylmethanethione (DFPM) inhibits ABA responses by activating effector-triggered immune signal transduction in Arabidopsis. In addition to the known function of DFPM as an antagonist of ... ...

    Abstract The small compound [5-(3,4-dichlorophenyl) furan-2-yl]-piperidine-1-ylmethanethione (DFPM) inhibits ABA responses by activating effector-triggered immune signal transduction in Arabidopsis. In addition to the known function of DFPM as an antagonist of ABA signaling, DFPM causes accession-specific root growth arrest in Arabidopsis Columbia-0 via the TIR-NLR protein
    Language English
    Publishing date 2023-08-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life13091797
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Arabidopsis

    Park, Eun-Joo / Kim, Tae-Houn

    International journal of molecular sciences

    2021  Volume 22, Issue 15

    Abstract: Plants have evolutionarily established resistance responses to a variety of abiotic stress conditions, in which ABA mediates the integrated regulation of these stress responses. Numerous proteins function at the transcription level or at the protein ... ...

    Abstract Plants have evolutionarily established resistance responses to a variety of abiotic stress conditions, in which ABA mediates the integrated regulation of these stress responses. Numerous proteins function at the transcription level or at the protein level when contributing to controls of the ABA signaling process. Although osmotin is identified as a salt-inducible protein, its function in the abiotic stress response is yet to be elucidated. To examine the role of Arabidopsis
    MeSH term(s) Abscisic Acid/metabolism ; Arabidopsis/metabolism ; Arabidopsis/physiology ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Gene Expression Regulation, Plant ; Glutamate-5-Semialdehyde Dehydrogenase/genetics ; Multienzyme Complexes/genetics ; Phosphotransferases (Alcohol Group Acceptor)/genetics ; Proline/analysis ; Proteolysis ; Signal Transduction ; Stress, Physiological
    Chemical Substances Arabidopsis Proteins ; Atosm34 protein, Arabidopsis ; Multienzyme Complexes ; delta(1)-pyrroline-5-carboxylate synthetase, Arabidopsis ; Abscisic Acid (72S9A8J5GW) ; Proline (9DLQ4CIU6V) ; Glutamate-5-Semialdehyde Dehydrogenase (EC 1.2.1.41) ; Phosphotransferases (Alcohol Group Acceptor) (EC 2.7.1.-)
    Language English
    Publishing date 2021-07-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22157915
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Thaumatin-like genes function in the control of both biotic stress signaling and ABA signaling pathways.

    Park, Eun Joo / Kim, Tae-Houn

    Biochemical and biophysical research communications

    2021  Volume 567, Page(s) 17–21

    Abstract: Thaumatin was isolated as a sweet-tasting protein. Arabidopsis has over 20 Thaumatin-Like Protein (TLP)/Osmoti-Like Protein (OLP) genes that belong to the PR5 family. Although biotic stress-related functions of TLPs have been reported from transgenic ... ...

    Abstract Thaumatin was isolated as a sweet-tasting protein. Arabidopsis has over 20 Thaumatin-Like Protein (TLP)/Osmoti-Like Protein (OLP) genes that belong to the PR5 family. Although biotic stress-related functions of TLPs have been reported from transgenic lines expressing TLPs, it is nonetheless necessary to investigate genetic phenotypes produced by defects in the TLP genes. In this report, four TLP genes were selected based on sequence similarities (Thau1/2/3/4), and the corresponding mutant thau1/2/3/4 was examined for biotic and abiotic stress responses. The thau1/2/3/4 mutant showed increased susceptibility to the Pseudomonas syringae pv. tomato DC3000 infection, and reduced sensitivity to the ABA and drought stress treatments. Each of the four thaumatin genes showed different gene expression patterns for ABA treatment. Moreover, ABA-inductions of Thau1/2/3/4 were largely dependent on the intact ABA signaling pathway mediated by PYR/PYL receptors. Among the many ABA-responsive genes affected by the defects of Thau1/2/3/4, reduced expression of P5CS1 with decreased accumulation phenotype of prolines indicates that compromised proline synthesis may be associated with the stress phenotypes of thau1/2/3/4. Our data suggest that Thau1/2/3/4 have a function in both biotic stress and abiotic stress signal transduction through the regulation of proline synthesis.
    MeSH term(s) Abscisic Acid/metabolism ; Arabidopsis/genetics ; Arabidopsis/growth & development ; Arabidopsis/metabolism ; Arabidopsis/microbiology ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Droughts ; Gene Expression Regulation, Plant ; Genes, Plant ; Germination ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/growth & development ; Plants, Genetically Modified/metabolism ; Plants, Genetically Modified/microbiology ; Pseudomonas syringae/physiology ; Signal Transduction ; Stress, Physiological
    Chemical Substances ATLP-1 protein, Arabidopsis ; Arabidopsis Proteins ; Abscisic Acid (72S9A8J5GW)
    Language English
    Publishing date 2021-06-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2021.06.012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Arabidopsis OSMOTIN 34 Functions in the ABA Signaling Pathway and Is Regulated by Proteolysis

    Eun-Joo Park / Tae-Houn Kim

    International Journal of Molecular Sciences, Vol 22, Iss 7915, p

    2021  Volume 7915

    Abstract: Plants have evolutionarily established resistance responses to a variety of abiotic stress conditions, in which ABA mediates the integrated regulation of these stress responses. Numerous proteins function at the transcription level or at the protein ... ...

    Abstract Plants have evolutionarily established resistance responses to a variety of abiotic stress conditions, in which ABA mediates the integrated regulation of these stress responses. Numerous proteins function at the transcription level or at the protein level when contributing to controls of the ABA signaling process. Although osmotin is identified as a salt-inducible protein, its function in the abiotic stress response is yet to be elucidated. To examine the role of Arabidopsis OSMOTIN 34 ( OSM34 ) in the ABA signaling pathway, a deletion mutant osm34 generated by a CRISPR/Cas9 system and the double mutant osm34 osml ( osmotin 34-like ) were analyzed for various ABA responses. Both osm34 and osm34 osml showed reduced levels of ABA responses in seeds and leaves. Moreover, proline level and expression of the proline biosynthesis gene P5CS1 was significantly reduced in osm34 osml. Interestingly, OSM34 binds to SKP2A, an F-Box protein whose transcription is induced by ABA. The protein stability of OSM34 was determined to be under the control of the 26S proteasome. In conclusion, our data suggest that OSM34 functions as a positive regulator in the generation of ABA responses and is under post-translational control.
    Keywords OSMOTIN 34 ; OSMOTIN 34-like ; abscisic acid ; F-Box gene ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580 ; 572
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Alternative Splicing for Improving Abiotic Stress Tolerance and Agronomic Traits in Crop Plants

    Kim, Seojung / Kim, Tae-Houn

    Journal of plant biology. 2020 Dec., v. 63, no. 6

    2020  

    Abstract: Most eukaryotic genes undergo various post-transcriptional processing events before being translated into proteins. Alternative splicing (AS) is one such event and is an essential mechanism in post-transcriptional gene regulation that allows multiple ... ...

    Abstract Most eukaryotic genes undergo various post-transcriptional processing events before being translated into proteins. Alternative splicing (AS) is one such event and is an essential mechanism in post-transcriptional gene regulation that allows multiple mRNA variants to be expressed from a single pre-mRNA, thereby expending the functional capacity of a gene as well as the organismal complexity. With the advancement of next-generation sequencing technologies, extensive transcriptomic resources in plant species have determined crucial roles of AS in the regulation of developmental processes and adaption to environmental stresses. We review here recent studies of AS events and splicing factors that specifically affect abiotic-stress tolerance in crop plants, including other agricultural traits. Understanding how alternative splicing modulates plant development and abiotic-stress responses may provide new insights for improving the environmental fitness and productivity of crop plants.
    Keywords abiotic stress ; agronomic traits ; alternative splicing ; crops ; genes ; high-throughput nucleotide sequencing ; plant development ; proteins ; stress tolerance ; transcriptomics
    Language English
    Dates of publication 2020-12
    Size p. 409-420.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    Note NAL-light ; Review
    ZDB-ID 2262362-0
    ISSN 1867-0725 ; 1226-9239
    ISSN (online) 1867-0725
    ISSN 1226-9239
    DOI 10.1007/s12374-020-09282-2
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Thaumatin-like genes function in the control of both biotic stress signaling and ABA signaling pathways

    Park, Eun Joo / Kim, Tae-Houn

    Biochemical and biophysical research communications. 2021 Aug. 27, v. 567

    2021  

    Abstract: Thaumatin was isolated as a sweet-tasting protein. Arabidopsis has over 20 Thaumatin-Like Protein (TLP)/Osmoti-Like Protein (OLP) genes that belong to the PR5 family. Although biotic stress-related functions of TLPs have been reported from transgenic ... ...

    Abstract Thaumatin was isolated as a sweet-tasting protein. Arabidopsis has over 20 Thaumatin-Like Protein (TLP)/Osmoti-Like Protein (OLP) genes that belong to the PR5 family. Although biotic stress-related functions of TLPs have been reported from transgenic lines expressing TLPs, it is nonetheless necessary to investigate genetic phenotypes produced by defects in the TLP genes. In this report, four TLP genes were selected based on sequence similarities (Thau1/2/3/4), and the corresponding mutant thau1/2/3/4 was examined for biotic and abiotic stress responses. The thau1/2/3/4 mutant showed increased susceptibility to the Pseudomonas syringae pv. tomato DC3000 infection, and reduced sensitivity to the ABA and drought stress treatments. Each of the four thaumatin genes showed different gene expression patterns for ABA treatment. Moreover, ABA-inductions of Thau1/2/3/4 were largely dependent on the intact ABA signaling pathway mediated by PYR/PYL receptors. Among the many ABA-responsive genes affected by the defects of Thau1/2/3/4, reduced expression of P5CS1 with decreased accumulation phenotype of prolines indicates that compromised proline synthesis may be associated with the stress phenotypes of thau1/2/3/4. Our data suggest that Thau1/2/3/4 have a function in both biotic stress and abiotic stress signal transduction through the regulation of proline synthesis.
    Keywords Arabidopsis ; Pseudomonas syringae pv. tomato ; biotic stress ; gene expression ; genetically modified organisms ; mutants ; phenotype ; proline ; protein sweeteners ; research ; signal transduction ; water stress
    Language English
    Dates of publication 2021-0827
    Size p. 17-21.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 205723-2
    ISSN 0006-291X ; 0006-291X
    ISSN (online) 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2021.06.012
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Fine-Tuning of Gene Expression by tRNA-Derived Fragments during Abiotic Stress Signal Transduction.

    Park, Eun Joo / Kim, Tae-Houn

    International journal of molecular sciences

    2018  Volume 19, Issue 2

    Abstract: When plants are subjected to unfavorable environmental conditions, overall gene expression in stressed cells is altered from a programmed pattern for normal development to an adaptive pattern for survival. Rapid changes in plant gene expression include ... ...

    Abstract When plants are subjected to unfavorable environmental conditions, overall gene expression in stressed cells is altered from a programmed pattern for normal development to an adaptive pattern for survival. Rapid changes in plant gene expression include production of stress responsive proteins for protection as well as reduction of irrelevant proteins to minimize energy consumption during growth. In addition to the many established mechanisms known to modulate gene expression in eukaryotes, a novel strategy involving tRNA-derived fragments (tRFs) was recently reported to control gene expression. In animals, tRFs are shown to play a certain role in infected or cancer cells. However, tRFs are expected to function in the regulation of gene expression against abiotic stress conditions in plants. Moreover, the underlying mechanism linking up-regulation of tRFs under stress conditions with the stress tolerant response remains unknown. In this review, the biogenesis and putative function of diverse tRFs in abiotic stress signaling are discussed with a focus on tRFs as a transcriptional/post-transcriptional/translational regulator.
    MeSH term(s) Abscisic Acid/metabolism ; Gene Expression Regulation ; Gene Silencing ; Plants/genetics ; Plants/metabolism ; RNA Processing, Post-Transcriptional ; RNA, Small Untranslated/genetics ; RNA, Transfer/genetics ; Ribonuclease III/metabolism ; Signal Transduction ; Stress, Physiological/genetics
    Chemical Substances RNA, Small Untranslated ; Abscisic Acid (72S9A8J5GW) ; RNA, Transfer (9014-25-9) ; Ribonuclease III (EC 3.1.26.3)
    Language English
    Publishing date 2018-02-08
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms19020518
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  8. Article ; Online: Production of ABA responses requires both the nuclear and cytoplasmic functional involvement of PYR1.

    Park, EunJoo / Kim, Tae-Houn

    Biochemical and biophysical research communications

    2017  Volume 484, Issue 1, Page(s) 34–39

    Abstract: Abscisic acid (ABA) enhances stress tolerant responses in plants against unfavorable environmental conditions. In Arabidopsis, ABA promotes interactions between PYR/PYL/RCARs and PP2C, thereby allowing SnRK2s to phosphorylate downstream components ... ...

    Abstract Abscisic acid (ABA) enhances stress tolerant responses in plants against unfavorable environmental conditions. In Arabidopsis, ABA promotes interactions between PYR/PYL/RCARs and PP2C, thereby allowing SnRK2s to phosphorylate downstream components required for the regulation of gene expression or for gating ion channels. Because PYR1 is known to localize to nucleus and cytoplasm it is a question whether nuclear or cytoplasmic PYR1 confer different functions to the ABA signaling pathway, as has been previously shown for regulatory proteins. In order to answer this question, transgenic lines expressing nuclear PYR1 were generated in an ABA insensitive mutant background. Enforced nuclear expression of PYR1 was examined by confocal microscopy and western blot analysis. Physiological analyses of the transgenic lines demonstrated that nuclear PYR1 is sufficient to generate ABA responses, such as, the inhibition of seed germination, root growth inhibition, the induction of gene expression, and stomatal closing movement. However, for the full recovery of ABA responses in the mutant background cytoplasmic PYR1 was required. The study suggests both nuclear and cytoplasmic PYR1 participate in the control of ABA signal transduction.
    MeSH term(s) Abscisic Acid/metabolism ; Arabidopsis/genetics ; Arabidopsis/growth & development ; Arabidopsis/metabolism ; Arabidopsis Proteins/metabolism ; Cell Nucleus/metabolism ; Cytoplasm/metabolism ; Membrane Transport Proteins/metabolism ; Mutation
    Chemical Substances Arabidopsis Proteins ; Membrane Transport Proteins ; Pyr1 protein, Arabidopsis ; Abscisic Acid (72S9A8J5GW)
    Language English
    Publishing date 2017-02-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2017.01.082
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Plant stress surveillance monitored by ABA and disease signaling interactions.

    Kim, Tae-Houn

    Molecules and cells

    2012  Volume 33, Issue 1, Page(s) 1–7

    Abstract: Abiotic and biotic stresses are the major factors that negatively impact plant growth. In response to abiotic environmental stresses such as drought, plants generate resistance responses through abscisic acid (ABA) signal transduction. In addition to the ...

    Abstract Abiotic and biotic stresses are the major factors that negatively impact plant growth. In response to abiotic environmental stresses such as drought, plants generate resistance responses through abscisic acid (ABA) signal transduction. In addition to the major role of ABA in abiotic stress signaling, ABA signaling was reported to downregulate biotic stress signaling. Conversely recent findings provide evidence that initial activation of plant immune signaling inhibits subsequent ABA signal transduction. Stimulation of effector-triggered disease response can interfere with ABA signal transduction via modulation of internal calcium-dependent signaling pathways. This review overviews the interactions of abiotic and biotic stress signal transduction and the mechanism through which stress surveillance system operates to generate the most efficient resistant traits against various stress condition.
    MeSH term(s) Abscisic Acid/metabolism ; Cell Communication/physiology ; Plant Diseases ; Signal Transduction ; Stress, Physiological
    Chemical Substances Abscisic Acid (72S9A8J5GW)
    Language English
    Publishing date 2012-01-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1148964-9
    ISSN 0219-1032 ; 1016-8478
    ISSN (online) 0219-1032
    ISSN 1016-8478
    DOI 10.1007/s10059-012-2299-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Fine-Tuning of Gene Expression by tRNA-Derived Fragments during Abiotic Stress Signal Transduction

    Eun Joo Park / Tae-Houn Kim

    International Journal of Molecular Sciences, Vol 19, Iss 2, p

    2018  Volume 518

    Abstract: When plants are subjected to unfavorable environmental conditions, overall gene expression in stressed cells is altered from a programmed pattern for normal development to an adaptive pattern for survival. Rapid changes in plant gene expression include ... ...

    Abstract When plants are subjected to unfavorable environmental conditions, overall gene expression in stressed cells is altered from a programmed pattern for normal development to an adaptive pattern for survival. Rapid changes in plant gene expression include production of stress responsive proteins for protection as well as reduction of irrelevant proteins to minimize energy consumption during growth. In addition to the many established mechanisms known to modulate gene expression in eukaryotes, a novel strategy involving tRNA-derived fragments (tRFs) was recently reported to control gene expression. In animals, tRFs are shown to play a certain role in infected or cancer cells. However, tRFs are expected to function in the regulation of gene expression against abiotic stress conditions in plants. Moreover, the underlying mechanism linking up-regulation of tRFs under stress conditions with the stress tolerant response remains unknown. In this review, the biogenesis and putative function of diverse tRFs in abiotic stress signaling are discussed with a focus on tRFs as a transcriptional/post-transcriptional/translational regulator.
    Keywords tRNA derived fragment ; abscisic acid ; abiotic stress ; post-transcriptional gene silencing ; plant ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2018-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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