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Article ; Online: Finance Allocation and Utilization as Predictor of Sports Development

Afshan Jabeen

Global Educational Studies Review 5(1) 10-18

A Case Study of Mianwali and Layyah District

2020

Abstract: Sports and Games in the Government colleges of different districts ( Mianwali & Layyah) of southern Punjab have been on the significant decline especially in female folk. Objective of this research was to analyze the allocation and utilization of sports ...

Abstract	`Sports and Games in the Government colleges of different districts ( Mianwali & Layyah) of southern Punjab have been on the significant decline especially in female folk. Objective of this research was to analyze the allocation and utilization of sports funds for female sports development at college level. Finance allocation and utilization are the pivots around which the particular study revolved. A sample of 34, LPEs (17) and senior clerks (17) working in government colleges for women were selected by using a convenient sampling technique. Two self-developed on 3-point Likert type scale consisting of 19 and 10 items were used for collecting the data. One for senior clerks and 2nd for lecturers in Physical Education (LPEs). The obtained data was analyzed by using one sample T-test. The study results found that the sports funds allocation as per the listed pupils in different five years was depicted enough to some extent while the allocated amount does not utilize properly for the promotion of college sports. So it was recommended that the college principals should be trained in the arena of fiscal management and use the allocated sports funds for the promotion of female sports only.
Keywords	Finance ; Allocation ; Utilization ; Sports ; Development
Subject code	796
Language	English
Publishing date	2020-03-30
Publishing country	eu
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Spectrum of Colorectal Polyps in Southern India

Afshan Jabeen / V Vijay Sreedhar

Journal of Clinical and Diagnostic Research, Vol 17, Iss 7, Pp 01-

A Twin Centre Ambispective Study

2023 Volume 04

Abstract: Introduction: Intestinal polyps are grossly visible tumours which protrude (into lumen of gastrointestinal tract). Majority of the polyps are benign in the paediatric age group. However in adults, they may be harbingers of more serious genetic, familial ... ...

Abstract	Introduction: Intestinal polyps are grossly visible tumours which protrude (into lumen of gastrointestinal tract). Majority of the polyps are benign in the paediatric age group. However in adults, they may be harbingers of more serious genetic, familial or developmental disorders and some of them may be premalignant. Aim: Our aim was to study the distribution, histological varieties of colorectal polyps and to examine dysplasia in adenomatous polyps and its determinants. Materials and Methods: This is a four years retrospective and one year prospective study from June 2017 to May 2022 conducted at Upgraded Department of pathology, Osmania General Hospital and a tertiary care centre, Shadan institute of medical sciences, Hyderabad. A total of 110 cases of polyps were studied which included 62 cases in adults and 48 cases in children (0-12 years). Predictors of dysplasia in adenomatous polyps were studied. Results were analysed using PSPP software. Results: There were 62.73% (69) males and 37.27% (41) females. The commonest clinical presentation was bleeding from rectum 64.54% (71) cases. Rectosigmoid was the commonest site in 83.64% (92) cases. Juvenile polyps were the commonest in children and constituted 89.58% (43 cases). In adults, there were 63.07% (41) adenomatous polyps. A statistically significant association has been observed between high grade dysplasia and size (p-value 0.015) and villous architecture (p-value 0.02). There was no statistically significant association with site (p-value 0.2). Conclusion: A rising incidence of Colorectal Cancer (CRC) has necessitated identification of preneoplastic Adenomatous polyps which may progress to carcinoma via the Adenomacarcinoma sequence. Polyps occurring as part of polyposis syndromes have increased neoplastic potential. Their detection and treatment carries significant implications both for the patients and their families. In India, there is a need for screening colonoscopy in asymptomatic individuals which can be a potential cancer prevention tool.
Keywords	adenomas ; benign ; dysplasia ; juvenile polyps ; villous adenomas ; Medicine ; R
Subject code	571
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	JCDR Research and Publications Private Limited
Document type	Article ; Online
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Article ; Online: Molecular basis for the increased activity of ZMS-2 serine protease in the presence of metal ions and hydrogen peroxide.

Khan, Zahoor / Tanoeyadi, Samuel / Jabeen, Nusrat / Shafique, Maryam / Naz, Sehar Afshan / Mahmud, Taifo

Journal of inorganic biochemistry

2024 Volume 256, Page(s) 112566

Abstract: Serine proteases are important enzymes widely used in commercial products and industry. Recently, we identified a new serine protease from the desert bacterium Bacillus subtilis ZMS-2 that showed enhanced activity in the presence of ... ...

Abstract	Serine proteases are important enzymes widely used in commercial products and industry. Recently, we identified a new serine protease from the desert bacterium Bacillus subtilis ZMS-2 that showed enhanced activity in the presence of Zn
Language	English
Publishing date	2024-04-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	162843-4
ISSN	1873-3344 ; 0162-0134
ISSN (online)	1873-3344
ISSN	0162-0134
DOI	10.1016/j.jinorgbio.2024.112566
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Article: In-vitro evaluation of virulence markers and antifungal resistance of clinical Candida albicans strains isolated from Karachi, Pakistan.

Jabeen, Gul / Naz, Sehar Afshan / Rangel, Drauzio E N / Jabeen, Nusrat / Shafique, Maryam / Yasmeen, Kousar

Fungal biology

2023 Volume 127, Issue 7-8, Page(s) 1241–1249

Abstract: Candidiasis is a significant fungal infection with high mortality and morbidity rates worldwide. Candida albicans is the most dominant species responsible for causing different manifestations of candidiasis. Certain virulence traits as well as its ... ...

Abstract	Candidiasis is a significant fungal infection with high mortality and morbidity rates worldwide. Candida albicans is the most dominant species responsible for causing different manifestations of candidiasis. Certain virulence traits as well as its resistance to antifungal drugs contribute to the pathogenesis of this yeast. This study was designed to determine the production of some virulence factors, such as biofilm formation and extracellular hydrolytic enzymes (esterase, coagulase, gelatinase, and catalase) by this fungus, as well as its antifungal resistance profile. A total of 304 clinical C. albicans isolates obtained from different clinical specimens were identified by a conventional diagnostic protocol. The antifungal susceptibility of C. albicans strains was determined by disk diffusion technique against commercially available antifungal disks, such as nystatin 50 μg, amphotericin B 100 unit, fluconazole 25 μg, itraconazole 10 μg, ketoconazole 10 μg, and voriconazole 1 μg. The assessment of biofilm formation was determined by the tube staining assay and spectrophotometry. Gelatinase, coagulase, catalase, and esterase enzyme production was also detected using standard techniques. A total of 66.1% (201/304) and 28.9% (88/304) of C. albicans strains were susceptible-dose dependent (SDD) to nystatin and itraconazole, respectively. Among the antifungal drugs, C. albicans strains showed high resistance to ketoconazole 24.7% (75/304); however, no statistically significant relationship between the clinical origin of C. albicans isolates and antifungal drug resistance pattern was detected. For virulence factors, the majority of the C. albicans strains actively produced biofilm and all hydrolytic enzymes. Biofilm formation was demonstrated by 88% (267/304) of the strains with a quantitative mean value 0.1762 (SD ± 0.08293). However, 100% (304/304) of isolates produced catalase enzyme, 69% (211/304) produced coagulase, 66% (197/304) produced gelatinase, and 52% (157/304) produced esterase enzyme. A significant relationship between the source of specimens and biofilm formation by C. albicans was observed; nevertheless, there was no significant relationship between different sources of C. albicans strains and the production of different enzymatic virulence factors. The study found that C. albicans strains have excellent potential to produce virulence markers and resistance to antifungals, which necessitates surveillance of these opportunistic pathogens to minimize the chances of severe invasive infections.
MeSH term(s)	Humans ; Antifungal Agents/pharmacology ; Candida albicans ; Itraconazole/pharmacology ; Catalase ; Nystatin/pharmacology ; Virulence ; Ketoconazole ; Pakistan ; Coagulase ; Candida ; Candidiasis/microbiology ; Esterases ; Virulence Factors ; Drug Resistance, Fungal ; Microbial Sensitivity Tests ; Gelatinases
Chemical Substances	Antifungal Agents ; Itraconazole (304NUG5GF4) ; Catalase (EC 1.11.1.6) ; Nystatin (1400-61-9) ; Ketoconazole (R9400W927I) ; Coagulase ; Esterases (EC 3.1.-) ; Virulence Factors ; Gelatinases (EC 3.4.24.-)
Language	English
Publishing date	2023-04-14
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2532164-X
ISSN	1878-6162 ; 1878-6146
ISSN (online)	1878-6162
ISSN	1878-6146
DOI	10.1016/j.funbio.2023.04.003
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Article ; Online: Structural insights of novel mutational frames in Bromodomain Containing-2 gene (BRD2) in juvenile myoclonic epilepsy: bed, bench, and laptop profiles.

Kothapalli, Jyothinath / Prasad Uppu, Venkateswara / Munikumar, Manne / Kshirsagar, S V / Afshan Jabeen, Shaik / Sivanarayana, Gopalam

Epilepsy & behavior : E&B

2023 Volume 144, Page(s) 109282

Abstract: Purpose: Juvenile myoclonic epilepsy (JME) is an adolescent onset type of idiopathic generalized epilepsies. Bromodomain containing protein-2 gene (BRD2), a transcriptional regulatory protein, has a susceptible role in the expression of JME. Considering ...

Abstract	Purpose: Juvenile myoclonic epilepsy (JME) is an adolescent onset type of idiopathic generalized epilepsies. Bromodomain containing protein-2 gene (BRD2), a transcriptional regulatory protein, has a susceptible role in the expression of JME. Considering the polymorphic variations observed in exon 3 of the BRD2 gene, we evaluated the molecular interactions with anti-seizure medication in individuals diagnosed with JME. Methods: The genomic DNA was extracted from 5 mL of peripheral venous blood of JME participants (n = 55) and healthy control subjects (n = 55). Detailed anti-seizure medication and outcomes were noted during the study period. Identified novel mutations at nucleotide and protein sequences, compared by multiple sequence alignment. Wild-type (WT) and mutated-type (MT) structures were investigated for molecular docking and interactions with anti-seizure drugs. Results: A common variant at c.1707G>A was found among 23 participants, while a single variant at c.1663ins C was found in one participant. The deletion positions were observed at c.1890delA, c.1892A>T, c.1895A>T, c.1896G>T, c.1897T>C, c.1898T>C, c.1899C>T, c.1900G>T, c.1901C>T and c.1902A>T exhibiting stop codon after p.111Pro>stop; these variants resulted in a truncated protein. In silico analysis was conducted to validate changes; docking analysis showed that novel variant has a significant role in the interactions with anti-seizure drugs. Significance: Besides clinical and genetic outcomes, ∼5.45% unique genetical variations were observed in the participants. Significant mimicked at the binding site position (92-111) of human BRD2 ranges ∼8.2%, ∼16.4%, and ∼10.6%. Further, research is needed to identify the importance of polymorphism alterations at the binding site and their molecular interactions with anti-seizure drugs, which might be confirmed in a diverse population with JME.
MeSH term(s)	Adolescent ; Humans ; Myoclonic Epilepsy, Juvenile/genetics ; Myoclonic Epilepsy, Juvenile/epidemiology ; Molecular Docking Simulation ; Epilepsy, Generalized ; Polymorphism, Genetic ; Disease Susceptibility ; Transcription Factors/genetics
Chemical Substances	BRD2 protein, human ; Transcription Factors
Language	English
Publishing date	2023-06-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	2010587-3
ISSN	1525-5069 ; 1525-5050
ISSN (online)	1525-5069
ISSN	1525-5050
DOI	10.1016/j.yebeh.2023.109282
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Article ; Online: In-vitro evaluation of virulence markers and antifungal resistance of clinical Candida albicans strains isolated from Karachi, Pakistan

Jabeen, Gul / Naz, Sehar Afshan / Rangel, Drauzio E.N. / Jabeen, Nusrat / Shafique, Maryam / Yasmeen, Kousar

Fungal Biology.

2023

Abstract: Candidiasis is a significant fungal infection with high mortality and morbidity rates worldwide. Candida albicans is the most dominant specie responsible for causing different manifestations of candidiasis. Certain virulence traits as well as its ... ...

Abstract	Candidiasis is a significant fungal infection with high mortality and morbidity rates worldwide. Candida albicans is the most dominant specie responsible for causing different manifestations of candidiasis. Certain virulence traits as well as its resistance to antifungal drugs contribute to the pathogenesis of this yeast fungus. This study was designed to determine the production of some virulence factors, such as biofilm formation and extracellular hydrolytic enzymes (esterase, coagulase, gelatinase, and catalase) by this fungus, as well as its antifungal resistance profile. A total of 304 clinical C. albicans isolates obtained from different clinical specimens were identified by a conventional diagnostic protocol. The antifungal susceptibility of C. albicans strains was determined by disk diffusion technique against commercially available antifungal disks, such as nystatin 50 μg, amphotericin B 100 unit, fluconazole 25 μg, itraconazole 10 μg, ketoconazole 10 μg, and voriconazole 1 μg. The assessment of biofilm formation was determined by the tube staining assay and spectrophotometry. Gelatinase, coagulase, catalase, and esterase enzyme production was also detected using standard techniques. A total of 66.1% (201/304) and 28.9% (88/304) of C. albicans strains were susceptible-dose dependent (SDD) to nystatin and itraconazole. Among the antifungal drugs, C. albicans strains showed high resistance to ketoconazole 24.7% (75/304); however, no statistically significant relationship between the clinical origin of C. albicans isolates and antifungal drug resistance pattern was detected. For virulence factors, the majority of the C. albicans strains actively produced biofilm and all hydrolytic enzymes. Biofilm formation was demonstrated by 88% (267/304) of the strains with a quantitative mean value 0.1762 (SD ± 0.08293). However, 100% (304/304) of isolates produced catalase enzyme, 69% (211/304) produced coagulase, 66% (197/304) produced gelatinase, and 52% (157/304) produced esterase enzyme. A significant relationship between the source of specimens and biofilm formation by C. albicans was observed; nevertheless, there was no significant relationship between different sources of C. albicans strains and the production of different enzymatic virulence factors. The study found that C. albicans strains have excellent potential to produce virulence markers and resistance to antifungals, which necessitates surveillance of these opportunistic pathogens to minimize the chances of severe invasive infections.
Keywords	Candida albicans ; amphotericin B ; biofilm ; candidiasis ; catalase ; drug resistance ; esterases ; fluconazole ; itraconazole ; ketoconazole ; monitoring ; morbidity ; mortality ; mycology ; nystatin ; pathogenesis ; spectroscopy ; virulence ; yeasts ; Pakistan ; Virulence factors ; Hydrolytic enzymes ; Antifungal resistance
Language	English
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Pre-press version
ZDB-ID	2532164-X
ISSN	1878-6162 ; 1878-6146
ISSN (online)	1878-6162
ISSN	1878-6146
DOI	10.1016/j.funbio.2023.04.003
Database	NAL-Catalogue (AGRICOLA)

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Article: Understanding Demyelination in Leprosy Neuropathy: A Nerve Biopsy Analysis.

Hassan, Rubina / Hui, Monalisa / Sireesha, Yareeda / Afshan, Jabeen / Meena, A K / Uppin, Megha S

Annals of Indian Academy of Neurology

2020 Volume 23, Issue 6, Page(s) 829–832

Language	English
Publishing date	2020-06-05
Publishing country	India
Document type	Journal Article
ZDB-ID	2240174-X
ISSN	1998-3549 ; 0972-2327
ISSN (online)	1998-3549
ISSN	0972-2327
DOI	10.4103/aian.AIAN_553_19
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Article ; Online: Characterization and Pilot-Scale Application of the ZMS-2 Serine Protease with Novel Properties for the Eco-friendly Leather Processing.

Khan, Zahoor / Shafique, Maryam / Tanoeyadi, Samuel / Solangi, Barkat Ali / Khan, Sumera Afzal / Jabeen, Nusrat / Nawaz, Hafiz Rub / Naz, Sehar Afshan / Mahmud, Taifo

Applied biochemistry and biotechnology

2023

Abstract: Microbial alkaline proteases are dominating the global enzyme market with a share of over 65% due to their multifarious catalytic potentials. Hence, production of proteases with novel properties of commercial significance is highly desirable to meet the ... ...

Abstract	Microbial alkaline proteases are dominating the global enzyme market with a share of over 65% due to their multifarious catalytic potentials. Hence, production of proteases with novel properties of commercial significance is highly desirable to meet the global enzyme demand. Here, we report the purification, characterization, and pilot-scale application of a serine protease from the desert soil bacterium Bacillus subtilis ZMS-2 with novel properties as dehairing agent in leather processing. The enzyme was purified 16.5-fold with a specific activity of 1543.5 U mg
Language	English
Publishing date	2023-11-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	392344-7
ISSN	1559-0291 ; 0273-2289
ISSN (online)	1559-0291
ISSN	0273-2289
DOI	10.1007/s12010-023-04790-6
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Article: Demographic variation and risk factors regarding breast cancer among females in Southern Punjab, Pakistan.

Ahmad, Waheed / Firasat, Sabika / Akhtar, Muhammad Sohail / Afshan, Kiran / Jabeen, Kaukab / Amjad, Rubina Ali

JPMA. The Journal of the Pakistan Medical Association

2021 Volume 71, Issue 7, Page(s) 1749–1756

Abstract: Objective: To explore epidemiology, clinical profiles and contribution of reproductive and non-reproductive risk factors in breast cancer development.: Methods: The case-control study was conducted from October 2017 to March 2018 at Quaid-i-Azam ... ...

Abstract	Objective: To explore epidemiology, clinical profiles and contribution of reproductive and non-reproductive risk factors in breast cancer development. Methods: The case-control study was conducted from October 2017 to March 2018 at Quaid-i-Azam University, Islamabad, Pakistan, and comprised breast cancer patients and age-matched controls recruited from the Bahawalpur Institute of Nuclear Medicine and Oncology, and the Bahawal Victoria Hospital, Bahawalpur. Socio-demographic data, family history of cancer, reproductive health and lifestyle factors were recorded using a structured questionnaire. Data was analysed using SPSS 21 and Stata/IC 14.1. Results: Of the 326 women, 163(50%) each were cases and controls. The mean age for both the groups was identical at 46.04±10.62 years. Positive family history and hypertension were significantly linked to increased breast cancer risk (p<0.05), while intense physical activity, increased anthropometric measurements and breastfeeding per child in months were inversely associated with the risk (p<0.05). Conclusions: Established risk factors for breast cancer were reaffirmed.
MeSH term(s)	Adult ; Breast Neoplasms/epidemiology ; Case-Control Studies ; Child ; Demography ; Female ; Humans ; Middle Aged ; Pakistan/epidemiology ; Risk Factors
Language	English
Publishing date	2021-08-18
Publishing country	Pakistan
Document type	Journal Article
ZDB-ID	603873-6
ISSN	0030-9982
ISSN	0030-9982
DOI	10.47391/JPMA.1091
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Article ; Online: Angiogenic potential and wound healing efficacy of chitosan derived hydrogels at varied concentrations of APTES in chick and mouse models.

Ara, Chaman / Jabeen, Sehrish / Afshan, Gul / Farooq, Ariba / Akram, Muhammad Sarfraz / Asmatullah / Islam, Atif / Ziafat, Shumaila / Nawaz, Bushra / Khan, Rafi Ullah

International journal of biological macromolecules

2022 Volume 202, Page(s) 177–190

Abstract: Chitosan (Cs) based biomaterials seem to be indispensable for neovasculogenesis and angiogenesis that ensure accelerated wound healing. Cs/poly (vinyl alcohol) (PVA) bio-constructs were cross-linked and investigated with varying concentrations of ... ...

Abstract	Chitosan (Cs) based biomaterials seem to be indispensable for neovasculogenesis and angiogenesis that ensure accelerated wound healing. Cs/poly (vinyl alcohol) (PVA) bio-constructs were cross-linked and investigated with varying concentrations of aminopropyltriethoxysilane (APTES). This study comprised of three phases: fabrication of hydrogels, characterization, assessment of angiogenic potential along with toxico-pathological effects, wound healing efficacy in chick and mice, respectively. The hydrogels were characterized by FTIR, SEM and TGA and the swelling response was examined in different solvents. The hydrogels swelling ratio was decreased with increasing amount of APTES, showed the highest swelling at acidic and basic pH while low swelling at neutral pH. Chorioallantoic membranes (CAM) assay was performed to study in-vivo angiogenesis, toxicological, morphological, biochemical and histological analyses in developing chicks. The results showed remarkably improved angiogenesis with little deviations in morphological, histological features and liver enzymes of chick embryos at higher concentrations of APTES. Besides, full thickness wounds were excised on mice dorsolateral skin to assess the wound healing. The rate of wound size reduction was significantly higher after topical application of hydrogels with elevated levels of crosslinker. Hence, the hydrogels showed enhanced angiogenesis, accelerated wound healing with little or no observable in-vivo toxicity.
MeSH term(s)	Animals ; Chick Embryo ; Chitosan/chemistry ; Chitosan/pharmacology ; Hydrogels/chemistry ; Hydrogels/pharmacology ; Mice ; Polyvinyl Alcohol/chemistry ; Polyvinyl Alcohol/pharmacology ; Propylamines ; Silanes ; Wound Healing
Chemical Substances	Hydrogels ; Propylamines ; Silanes ; Polyvinyl Alcohol (9002-89-5) ; Chitosan (9012-76-4) ; amino-propyl-triethoxysilane (L8S6UBW552)
Language	English
Publishing date	2022-01-14
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2022.01.047
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