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Article: Nitric oxide mitigates salt-induced oxidative stress in

Khator, Khushboo / Shekhawat, G S

2020 Volume 10, Issue 11, Page(s) 499

Abstract: The present investigation was designed to determine the interaction of nitric oxide with other antioxidants in relieving oxidative stress induced by NaCl at morphological, physiological and molecular level. 15 days old seedlings ... ...

Abstract	The present investigation was designed to determine the interaction of nitric oxide with other antioxidants in relieving oxidative stress induced by NaCl at morphological, physiological and molecular level. 15 days old seedlings of
Language	English
Publishing date	2020-10-31
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2600522-0
ISSN	2190-5738 ; 2190-572X
ISSN (online)	2190-5738
ISSN	2190-572X
DOI	10.1007/s13205-020-02493-x
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Article ; Online: Sutured Descemet Membrane Endothelial Keratoplasty in Patients With Complex Anatomy or Difficulty Positioning.

Li, Gavin / Shekhawat, Nakul S / Eghrari, Allen O

Cornea

2022 Volume 42, Issue 3, Page(s) 389–394

Abstract: Purpose: In Descemet membrane endothelial keratoplasty (DMEK), some patients cannot adhere to postoperative positioning requirements or lack anatomic support for a gas bubble in the anterior chamber. We describe a suturing technique that stabilizes the ... ...

Abstract	Purpose: In Descemet membrane endothelial keratoplasty (DMEK), some patients cannot adhere to postoperative positioning requirements or lack anatomic support for a gas bubble in the anterior chamber. We describe a suturing technique that stabilizes the graft postoperatively without tearing or tenting it. Methods: One to two 10-0 nylon sutures with a spatulated needle are used to secure DMEK grafts. Tied with minimal tension, the sutures traverse the corneal stroma at a steep, almost perpendicular angle and approach the graft from the limbus at an angle tangential to the circular graft to prevent vaulting the tissue off the central cornea. We report preoperative and postoperative best spectacle-corrected visual acuity, corneal pachymetry, and anterior segment optical coherence tomography confirming graft attachment in 3 patients. Results: In case 1 (aphakia, postvitrectomy, and positioning difficulty), pachymetry decreased from 637 μm preoperatively to 495 μm and best spectacle-corrected visual acuity improved from 20/70 to 20/50 at postoperative week 1. In case 2 (aphakia, limited follow-up, and medication adherence), pachymetry decreased from 1106 μm preoperatively to 783 μm at postoperative month 1. In case 3 (tube shunt and loss of gas bubble at 2 hours), slit-lamp examination at postoperative day 1 showed corneal clearing and graft attachment. Pachymetry decreased from over 795 μm preoperatively to 582 μm at postoperative month 1. Anterior segment optical coherence tomography confirmed graft attachment in all patients. Conclusions: Single or opposing tangentially oriented sutures can be safely applied to secure DMEK grafts postoperatively in challenging cases.
MeSH term(s)	Humans ; Descemet Membrane/surgery ; Endothelium, Corneal/transplantation ; Descemet Stripping Endothelial Keratoplasty/methods ; Visual Acuity ; Cornea ; Retrospective Studies ; Corneal Diseases/surgery
Language	English
Publishing date	2022-12-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	604826-2
ISSN	1536-4798 ; 0277-3740
ISSN (online)	1536-4798
ISSN	0277-3740
DOI	10.1097/ICO.0000000000003202
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Article ; Online: Tissue-specific variations of piperine in ten populations of Piper longum L.: bioactivities and toxicological profile.

Biswas, Protha / Pandey, Devendra Kumar / Shekhawat, Mahipal S / Dey, Abhijit / Malik, Tabarak

Scientific reports

2024 Volume 14, Issue 1, Page(s) 5062

Abstract: ... highest 16.362 mg/g in chemotype PL9) than the stem and leaf chemotypes. Both DPPH and ABTS antioxidant ...

Abstract	P. longum L., one of the most significant species of the genus Piperaceae, is most frequently employed in Indian-Ayurvedic and other traditional medicinal-systems for treating a variety of illnesses. The alkaloid piperine, is the key phytoconstituent of the plant, primarily responsible for its' pharmacological-impacts. The aim of the study is to analyse the intra-specific variation in piperine content among different chemotypes (PL1 to PL 30) and identify high piperine yielding chemotype (elite-chemotype) collected from 10 different geographical regions of West Bengal by validated HPTLC chromatography method. The study also focused on the pharmacological-screening to better understand the antioxidant activity of the methanol extracts of P. longum by DPPH and ABTS radical-scavenging activity and genotoxic activity by Allium cepa root tip assay. It was found that the P. longum fruit chemotypes contain high amount piperine (highest 16.362 mg/g in chemotype PL9) than the stem and leaf chemotypes. Both DPPH and ABTS antioxidant assays revealed that P. longum showed moderate radical-scavenging activity and the highest activity was found in PL9 (fruit) chemotype with IC
MeSH term(s)	Plant Extracts/pharmacology ; Plant Extracts/chemistry ; Piper/chemistry ; Antioxidants/pharmacology ; Alkaloids/pharmacology ; Alkaloids/analysis ; Benzodioxoles/pharmacology ; Piperidines ; Sulfonic Acids ; Benzothiazoles ; Polyunsaturated Alkamides
Chemical Substances	piperine (U71XL721QK) ; Plant Extracts ; Antioxidants ; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (28752-68-3) ; Alkaloids ; Benzodioxoles ; Piperidines ; Sulfonic Acids ; Benzothiazoles ; Polyunsaturated Alkamides
Language	English
Publishing date	2024-03-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-52297-9
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Book ; Conference proceedings: Potato: present & future

Shekhawat, G. S.

(proceedings of the National Symposium [on Potato: Present & Future] held at Modipuram during 1 - 3 March, 1993)

1994

Institution	Indian Potato Association
Event/congress	National Symposium on Potato: Present & Future (1993, Modipuram)
Author's details	Indian Potato Association. Ed.: G. S. Shekhawat
Keywords	Indien ; Kartoffelbau ; Kartoffelwirtschaft ; Kartoffelzüchtung ; Kartoffelsorte ; Biotechnologie
Subject	Kartoffel ; Technische Biochemie ; Technische Biologie ; Biotechnik ; Biotech ; Kartoffelanbau
Size	IV, 347 S. : graph. Darst.
Publishing place	Shimla
Publishing country	India
Document type	Book ; Conference proceedings
HBZ-ID	HT007536724
ISBN	81-900419-0-8 ; 978-81-900419-0-4
Database	Catalogue ZB MED Nutrition, Environment, Agriculture

In stock of ZB MED Bonn / Germany

Shelf mark	Loan status	Location
978/1080	available for loan (reading room)	Freihandmagazin (U1)

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Article ; Online: Structure-property relationships in critically connected (GeTe

Chahal, Shweta / Prabhudessai, Akila G / Shekhawat, Roopali / Vinoth, S / Ramesh, K

Dalton transactions (Cambridge, England : 2003)

2022 Volume 51, Issue 32, Page(s) 12100–12113

Abstract: Thermal, optical, mechanical and structural studies were carried out on glasses in the pseudo-binary joint ... ...

Abstract	Thermal, optical, mechanical and structural studies were carried out on glasses in the pseudo-binary joint GeTe
Language	English
Publishing date	2022-08-16
Publishing country	England
Document type	Journal Article
ZDB-ID	1472887-4
ISSN	1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
ISSN (online)	1477-9234 ; 1364-5447
ISSN	0300-9246 ; 1477-9226
DOI	10.1039/d2dt01969h
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Article ; Online: Impact of sensor-based wastewater irrigation on chemical soil health in chrysanthemum (Chrysanthemum morifolium)

ANKIT / D S GURJAR / K G ROSIN / KAPILA SHEKHAWAT / RITU JAIN

The Indian Journal of Agricultural Sciences, Vol 93, Iss

2023 Volume 6

Abstract: A field experiment was conducted to study the short-term impact of sensor-based wastewater irrigation scheduling on chemical soil health in chrysanthemum (Chrysanthemum morifolium Ramat.) at Water Technology Centre, ICAR-Indian Agricultural Research ... ...

Abstract	A field experiment was conducted to study the short-term impact of sensor-based wastewater irrigation scheduling on chemical soil health in chrysanthemum (Chrysanthemum morifolium Ramat.) at Water Technology Centre, ICAR-Indian Agricultural Research Institute (IARI), New Delhi during 2019–20. Experiment was conducted with following 6 treatments, viz. T1, Groundwater irrigation (GWI) at 25% Maximum Allowable Depletion (MAD), T2, GWI at 50% MAD, T3, GWI at 75% MAD, T4, Wastewater irrigation (WWI) at 25% MAD, T5, WWI at 50% MAD, T6, WWI at 75% MAD and was laid out in a randomized block design (RBD) in open field condition with 4 replications. Soil samples and quality of groundwater and wastewater were assessed during and after the experiment to quantify the chemical properties (pH, EC and OC), primary nutrients (NPK), micronutrients (Fe, Mn, Cu and Zn) and heavy metals (Ni, Pb, Cr and Cd). Results shows that pH and EC remains the same. Organic carbon, primary nutrient and micronutrients content significantly increased in wastewater irrigated soils and best result was observed in T5. Moreover, the content of heavy metals (Ni, Cr, Cd and Pb) in soils was significantly not changed with different wastewater irrigation treatments. Wastewater irrigation have beneficial impacts on soil chemical parameters in terms of higher amount of organic matter, primary and micronutrients in soil.
Keywords	Chrysanthemum ; FDR ; Heavy metals ; Irrigation scheduling ; MAD ; Wastewater ; Agriculture ; S
Subject code	571
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	Indian Council of Agricultural Research
Document type	Article ; Online
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Article: Sulfonated nitrogen and added-sulfur sources influence productivity, quality, and nutrient acquisition of soybean-wheat cropping system.

Rathore, Sanjay Singh / Sharma, K C / Shekhawat, Kapila / Babu, Subhash / Sanketh, G D / Singh, V K / Singh, Rajiv K / Upadhyay, Pravin Kumar / Hashim, Mohd / Jangir, Rameti / Singh, Harvir

Heliyon

2024 Volume 10, Issue 5, Page(s) e26815

Abstract: ... nutrient management. Increasing sulfur (S) deficiency is widespread, especially under oilseed-based cropping system ... Hence, to standardize the S requirement through customized fertilization, an experiment was conducted in completely ... the agronomic performance of sulfonated nitrogen (SN) in comparison to conventional S nutrient sources in SWCS ...

Abstract	Soybean-wheat is the predominant cropping system covering >2.5 Mha area in India. The lower productivity of soybean-wheat cropping system (SWCS), remains a serious concern primarily due to inadequate nutrient management. Increasing sulfur (S) deficiency is widespread, especially under oilseed-based cropping system. Hence, to standardize the S requirement through customized fertilization, an experiment was conducted in completely randomized block design (RBD) comprised of 12 nutrient sources, replicated thrice. The study aims to evaluate the agronomic performance of sulfonated nitrogen (SN) in comparison to conventional S nutrient sources in SWCS. The maximum soybean productivity was recorded under NPK + S through 40-0-0-13 (SN1), although NPK + 50% S (15 kg/ha) as basal and 50% (15 kg/ha) as top dressing through SN2 10-0-0-75 produced maximum wheat grain yield. When compared with no nitrogen (control), the application of 30 kg S ha
Language	English
Publishing date	2024-02-23
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e26815
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Article: Nitric oxide mitigates salt-induced oxidative stress in Brassica juncea seedlings by regulating ROS metabolism and antioxidant defense system

Khator, Khushboo / Shekhawat, G. S

3 Biotech. 2020 Nov., v. 10, no. 11

2020

Abstract	The present investigation was designed to determine the interaction of nitric oxide with other antioxidants in relieving oxidative stress induced by NaCl at morphological, physiological and molecular level. 15 days old seedlings of B. juncea were subjected to 50 mM NaCl alone, 100 μM SNP alone and in combination (SNP + NaCl) in hoagland growth medium for 96 h and to analyze the cellular homeostasis and salt tolerance mechanism via examining growth, stress parameters, enzymatic and non enzymatic antioxidants and expression level of NR. Exposure of 100 μM sodium nitroprusside to mustard seedling enhanced photosynthetic pigment content and prevented plant growth inhibition. Accumulation of MDA and H₂O₂ was more pronounced in individual NaCl treated seedling than in the combination of NaCl and SNP. Applying SNP enhanced NR activity by 1.70 folds and increased NO production by 2.26 folds than individual salt treated roots. Furthermore, the activities of CAT, GPX and NR act synergistically with endogenous NO level whereas APX work antagonistically. In addition, the study also demonstrates that NO regulated NaCl induced transcriptional expression of NR. Induction of BjNR in Indian mustard roots lead to enhanced the plant resistance against salinity stress. The present finding revealed that NO confers increased B. juncea tolerance to salt stress by stimulation of antioxidants and reestablishment of cellular redox status.
Keywords	Brassica juncea ; antioxidant activity ; culture media ; growth retardation ; homeostasis ; nitric oxide ; nitroprusside ; oxidative stress ; photosynthesis ; plant growth ; salt stress ; salt tolerance ; seedlings ; transcription (genetics)
Language	English
Dates of publication	2020-11
Size	p. 499.
Publishing place	Springer International Publishing
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2600522-0
ISSN	2190-5738 ; 2190-572X
ISSN (online)	2190-5738
ISSN	2190-572X
DOI	10.1007/s13205-020-02493-x
Database	NAL-Catalogue (AGRICOLA)

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Article: Cd- and Cu-induced phytotoxicity on 2–3 leaf stage of Cyamopsis tetragonoloba and its regulation by nitrate reductase and ROS quenching enzyme

Khator, Khushboo / Shekhawat, G. S

Acta physiologiae plantarum. 2020 July, v. 42, no. 7

2020

Abstract: The present study focused on Cd- and Cu-induced nitrate reductase activity and its relation to ROS scavenging enzyme in C. tetragonoloba. Hydroponically adapted seedling treated with different metal concentration (Cd and Cu) in the series from 10 to 100 ... ...

Abstract	The present study focused on Cd- and Cu-induced nitrate reductase activity and its relation to ROS scavenging enzyme in C. tetragonoloba. Hydroponically adapted seedling treated with different metal concentration (Cd and Cu) in the series from 10 to 100 µM. Seedlings were harvested after 96 h of metal treatment and analyze cellular homeostasis and metal tolerance mechanism by determining growth, stress and enzymatic parameters. The nitrate reductase activity was observed to be highest at 70 µM Cd and 50 µM Cu in cyamopsis roots, i.e., 2.31 and 2.59 times higher than control. Exogenous supplementation of SNP (100 µM) enhanced the NR activity in roots of C. tetragonoloba. Polymorphism between different metal treated leaves and roots were also observed. Higher polymorphism was observed in metal-treated roots in comparison to metal-treated leaves. The activity of other antioxidants except catalase (ascorbate peroxidase, guaiacol peroxidase and nitrate reductase was also recorded to be highest at 70 µM Cd and 50 µM Cu. Increased activity of Nitrate reductase and other antioxidants were directly associated with reduction in MDA and H₂O₂ level at 70 µM Cd and 50 µM Cu. The present investigation is highlighted the defensive role of Nitrate reductase in mitigating Cd and Cu induced toxicity by regulation of antioxidants and ROS metabolism. The study is important as this is the first report exemplifying the protective role of Nitrate reductase in detoxifying ROS in C. tetragonoloba under Cd and Cu stress.
Keywords	Cyamopsis tetragonoloba ; ascorbate peroxidase ; catalase ; homeostasis ; hydroponics ; leaves ; metabolism ; metal tolerance ; nitrate reductase ; peroxidase ; phytotoxicity ; protective effect ; seedlings
Language	English
Dates of publication	2020-07
Size	p. 120.
Publishing place	Springer Berlin Heidelberg
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	783102-x
ISSN	1861-1664 ; 0137-5881
ISSN (online)	1861-1664
ISSN	0137-5881
DOI	10.1007/s11738-020-03105-0
Database	NAL-Catalogue (AGRICOLA)

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Article: Intrauterine insemination versus fallopian tube sperm perfusion in non-tubal infertility.

Shekhawat, G S

Medical journal, Armed Forces India

2012 Volume 68, Issue 3, Page(s) 226–230

Abstract: Background: Controlled ovarian hyperstimulation (COH) combined with intrauterine insemination (IUI), using a volume of 0.5 ml of inseminate is commonly offered to couples with non-tubal sub fertility. Another method is Fallopian tube sperm perfusion ( ... ...

Abstract	Background: Controlled ovarian hyperstimulation (COH) combined with intrauterine insemination (IUI), using a volume of 0.5 ml of inseminate is commonly offered to couples with non-tubal sub fertility. Another method is Fallopian tube sperm perfusion (FSP) which is based on a pressure injection of 4 ml of sperm suspension while attempting to seal the cervix to prevent semen reflux. This technique ensures the presence of higher sperm density in the fallopian tubes at the time of ovulation than standard IUI. The aim of this study was to compare the efficiency of standard intrauterine insemination (IUI) and Fallopian tube sperm perfusion (FSP) in the treatment of non-tubal infertility. Methods: 200 consecutive patients with infertility in 404 stimulated cycles were included in the study. Those randomized to standard IUI included 100 patients in 184 cycles [158 clomiphene citrate/human menopausal gonadotrophin cycles and 26 Letrozole/FSH cycles exclusively for polycystic ovarian disease patients] (group A). Patients subjected to FSP included 100 patients in 220 cycles (193 clomiphene citrate/human menopausal gonadotrophin cycles and 27 Letrozole/FSH cycles exclusively for polycystic ovarian disease patients] (group B). Swim up semen preparation technique was used in all cases. Insemination was performed in both groups 34-37 h after hCG administration. Standard IUI was performed using 0.5 ml of inseminate. In FSP 4 ml inseminate was used. Results: In group A (184 IUI cycles in 100 patients), 22 clinical pregnancies (presence of gestational sac with fetal cardiac activity) occurred (11.95% per cycle over four cycles). In group B, (220 cycles of FSP in 100 patients), 48 clinical pregnancies occurred (21.81% per cycle over four cycles) and this difference was statistically significant (p < 0.05). Conclusions: For non-tubal sub fertility, the results indicate clear benefit for FSP (Fallopian tube sperm perfusion) over IUI (Intrauterine insemination).
Language	English
Publishing date	2012-05-12
Publishing country	India
Document type	Journal Article
ZDB-ID	196342-9
ISSN	0377-1237
ISSN	0377-1237
DOI	10.1016/j.mjafi.2012.02.013
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