Result 1 - 10 of total 42923
The Chicago medical journal
2023 Volume 25, Issue 16, Page(s) 532–537
| Language | English |
|---|---|
| Publishing date | 2023-07-06 |
| Publishing country | United States |
| Document type | Journal Article |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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2019 Volume 84, Issue 11, Page(s) e472–e474
| MeSH term(s) | Heart-Lung Machine/history ; History, 20th Century ; Humans ; United States |
|---|---|
| Language | English |
| Publishing date | 2019-02-12 |
| Publishing country | United States |
| Document type | Biography ; Historical Article ; Journal Article ; Portrait |
| ZDB-ID | 202465-2 |
| ISSN | 1555-9823 ; 0003-1348 |
| ISSN (online) | 1555-9823 |
| ISSN | 0003-1348 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Uk I Zs.106: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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PloS one
2019 Volume 14, Issue 3, Page(s) e0211600
Abstract: Tracking of clonal immunoglobulin V(D)J rearrangement sequences by next generation sequencing is ... variable rates of V(D)J sequence identification at baseline, which could limit tracking. Here, we aimed ... to define the factors influencing the identification of clonal V(D)J sequences. Bone marrow mononuclear ...
| Abstract | Tracking of clonal immunoglobulin V(D)J rearrangement sequences by next generation sequencing is highly sensitive for minimal residual disease in multiple myeloma. However, previous studies have found variable rates of V(D)J sequence identification at baseline, which could limit tracking. Here, we aimed to define the factors influencing the identification of clonal V(D)J sequences. Bone marrow mononuclear cells from 177 myeloma patients underwent V(D)J sequencing by the LymphoTrack assays (Invivoscribe). As a molecular control for tumor cell content, we sequenced the samples using our in-house myeloma panel myTYPE. V(D)J sequence clonality was identified in 81% of samples overall, as compared with 95% in samples where tumor-derived DNA was detectable by myTYPE. Clonality was detected more frequently in patients with lambda-restricted disease, mainly because of increased detection of kappa gene rearrangements. Finally, we describe how the tumor cell content of bone marrow aspirates decrease gradually in sequential pulls because of hemodilution: From the initial pull used for aspirate smear, to the final pull that is commonly used for research. In conclusion, baseline clonality detection rates of 95% or higher are feasible in multiple myeloma. Optimal performance depends on the use of good quality aspirates and/or subsequent tumor cell enrichment. |
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| MeSH term(s) | Aged ; Bone Marrow Cells/metabolism ; Female ; Humans ; Immunoglobulin Heavy Chains/genetics ; Immunoglobulin kappa-Chains/genetics ; Male ; Middle Aged ; Multiple Myeloma/diagnosis ; Multiple Myeloma/genetics ; Neoplasm, Residual/diagnosis ; Neoplasm, Residual/genetics ; Sequence Analysis, DNA ; V(D)J Recombination |
| Chemical Substances | Immunoglobulin Heavy Chains ; Immunoglobulin kappa-Chains |
| Language | English |
| Publishing date | 2019-03-22 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural |
| ISSN | 1932-6203 |
| ISSN (online) | 1932-6203 |
| DOI | 10.1371/journal.pone.0211600 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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PLoS ONE, Vol 14, Iss 3, p e
2019 Volume 0211600
Abstract: Tracking of clonal immunoglobulin V(D)J rearrangement sequences by next generation sequencing is ... variable rates of V(D)J sequence identification at baseline, which could limit tracking. Here, we aimed ... to define the factors influencing the identification of clonal V(D)J sequences. Bone marrow mononuclear ...
| Abstract | Tracking of clonal immunoglobulin V(D)J rearrangement sequences by next generation sequencing is highly sensitive for minimal residual disease in multiple myeloma. However, previous studies have found variable rates of V(D)J sequence identification at baseline, which could limit tracking. Here, we aimed to define the factors influencing the identification of clonal V(D)J sequences. Bone marrow mononuclear cells from 177 myeloma patients underwent V(D)J sequencing by the LymphoTrack assays (Invivoscribe). As a molecular control for tumor cell content, we sequenced the samples using our in-house myeloma panel myTYPE. V(D)J sequence clonality was identified in 81% of samples overall, as compared with 95% in samples where tumor-derived DNA was detectable by myTYPE. Clonality was detected more frequently in patients with lambda-restricted disease, mainly because of increased detection of kappa gene rearrangements. Finally, we describe how the tumor cell content of bone marrow aspirates decrease gradually in sequential pulls because of hemodilution: From the initial pull used for aspirate smear, to the final pull that is commonly used for research. In conclusion, baseline clonality detection rates of 95% or higher are feasible in multiple myeloma. Optimal performance depends on the use of good quality aspirates and/or subsequent tumor cell enrichment. |
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| Keywords | Medicine ; R ; Science ; Q |
| Subject code | 610 |
| Language | English |
| Publishing date | 2019-01-01T00:00:00Z |
| Publisher | Public Library of Science (PLoS) |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
2006 Volume 96, Issue 1, Page(s) 12304
Abstract: J/psi production in d + Au and p + p collisions at square root of S(NN) = 200 GeV has been measured ... by the PHENIX experiment at rapidities -2.2 < y < +2.4. The cross sections and nuclear dependence of J/psi ... A results and to theoretical models. The observed nuclear dependence in d + Au collisions is found to be ...
| Abstract | J/psi production in d + Au and p + p collisions at square root of S(NN) = 200 GeV has been measured by the PHENIX experiment at rapidities -2.2 < y < +2.4. The cross sections and nuclear dependence of J/psi production versus rapidity, transverse momentum, and centrality are obtained and compared to lower energy p + A results and to theoretical models. The observed nuclear dependence in d + Au collisions is found to be modest, suggesting that the absorption in the final state is weak and the shadowing of the gluon distributions is small and consistent with Dokshitzer-Gribov-Lipatov-Altarelli-Parisi-based parametrizations that fit deep-inelastic scattering and Drell-Yan data at lower energies. |
|---|---|
| Language | English |
| Publishing date | 2006-01-13 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 208853-8 |
| ISSN | 1079-7114 ; 0031-9007 |
| ISSN (online) | 1079-7114 |
| ISSN | 0031-9007 |
| DOI | 10.1103/PhysRevLett.96.012304 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Z 4' 58/153: Show issues |
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Molecular and cellular biology
2013 Volume 33, Issue 18, Page(s) 3568–3579
Abstract: V(D)J recombination is initiated by the RAG endonuclease, which introduces DNA double-strand breaks ... serine-threonine kinases that orchestrate the cellular responses to DNA DSBs. During V(D)J recombination, ATM and DNA-PKcs ...
| Abstract | V(D)J recombination is initiated by the RAG endonuclease, which introduces DNA double-strand breaks (DSBs) at the border between two recombining gene segments, generating two hairpin-sealed coding ends and two blunt signal ends. ATM and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) are serine-threonine kinases that orchestrate the cellular responses to DNA DSBs. During V(D)J recombination, ATM and DNA-PKcs have unique functions in the repair of coding DNA ends. ATM deficiency leads to instability of postcleavage complexes and the loss of coding ends from these complexes. DNA-PKcs deficiency leads to a nearly complete block in coding join formation, as DNA-PKcs is required to activate Artemis, the endonuclease that opens hairpin-sealed coding ends. In contrast to loss of DNA-PKcs protein, here we show that inhibition of DNA-PKcs kinase activity has no effect on coding join formation when ATM is present and its kinase activity is intact. The ability of ATM to compensate for DNA-PKcs kinase activity depends on the integrity of three threonines in DNA-PKcs that are phosphorylation targets of ATM, suggesting that ATM can modulate DNA-PKcs activity through direct phosphorylation of DNA-PKcs. Mutation of these threonine residues to alanine (DNA-PKcs(3A)) renders DNA-PKcs dependent on its intrinsic kinase activity during coding end joining, at a step downstream of opening hairpin-sealed coding ends. Thus, DNA-PKcs has critical functions in coding end joining beyond promoting Artemis endonuclease activity, and these functions can be regulated redundantly by the kinase activity of either ATM or DNA-PKcs. |
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| MeSH term(s) | Animals ; Ataxia Telangiectasia Mutated Proteins/chemistry ; Ataxia Telangiectasia Mutated Proteins/genetics ; Ataxia Telangiectasia Mutated Proteins/metabolism ; Catalytic Domain ; Cells, Cultured ; DNA Breaks, Double-Stranded ; DNA End-Joining Repair ; DNA-Activated Protein Kinase/chemistry ; DNA-Activated Protein Kinase/genetics ; DNA-Activated Protein Kinase/metabolism ; DNA-Binding Proteins/chemistry ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Endonucleases/metabolism ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Mice ; Nuclear Proteins/chemistry ; Nuclear Proteins/genetics ; Nuclear Proteins/metabolism ; Phosphorylation ; Precursor Cells, B-Lymphoid/metabolism ; Protein Interaction Domains and Motifs ; V(D)J Recombination |
| Chemical Substances | DNA-Binding Proteins ; Homeodomain Proteins ; Nuclear Proteins ; Rag2 protein, mouse ; RAG-1 protein (128559-51-3) ; Ataxia Telangiectasia Mutated Proteins (EC 2.7.11.1) ; Atm protein, mouse (EC 2.7.11.1) ; DNA-Activated Protein Kinase (EC 2.7.11.1) ; Prkdc protein, mouse (EC 2.7.11.1) ; Endonucleases (EC 3.1.-) ; Dclre1c protein, mouse (EC 3.1.-.-) |
| Language | English |
| Publishing date | 2013-07-08 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 779397-2 |
| ISSN | 1098-5549 ; 0270-7306 |
| ISSN (online) | 1098-5549 |
| ISSN | 0270-7306 |
| DOI | 10.1128/MCB.00308-13 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 1666: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
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2008 Volume 179, Issue 5, Page(s) 2067
| MeSH term(s) | Artificial Intelligence ; Humans ; Male ; Prostatic Neoplasms/diagnosis |
|---|---|
| Language | English |
| Publishing date | 2008-05 |
| Publishing country | United States |
| Document type | Comment ; Letter |
| ZDB-ID | 3176-8 |
| ISSN | 1527-3792 ; 0022-5347 |
| ISSN (online) | 1527-3792 |
| ISSN | 0022-5347 |
| DOI | 10.1016/j.juro.2008.01.053 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Ui VI Zs.91: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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| Zs.MO 331: Show issues |
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2012
Abstract: Studies in the fission yeast Schizosaccharomyces pombe (S. pombe) have done much to inform the view of heterochromatin and its control by the RNA interference (RNAi) machinery. Using cDNA synthesised from poly(A)-enriched RNA samples, numerous novel ... ...
| Abstract | Studies in the fission yeast Schizosaccharomyces pombe (S. pombe) have done much to inform the view of heterochromatin and its control by the RNA interference (RNAi) machinery. Using cDNA synthesised from poly(A)-enriched RNA samples, numerous novel ncRNA loci were discovered, and the 50 and 30 ends of many other genes were refined in previous studies. Although some of these transcripts may encode novel proteins the function of the majority is yet to be determined. The authors have used strand-specific deep sequencing of RNA, irrespective of poly(A) status, to reveal a highly structured antisense programme that modulates gene expression to dictate cell fate decisions during sexual differentiation. They show that an extensive and elaborate array of ncRNA production accompanies sexual differentiation in the fission yeast S. pombe. Experimental manipulation suggests that these transcripts specifically regulate the function of the target genes. |
|---|---|
| Language | English |
| Dates of publication | 2012-9999 |
| Size | Online-Ressource |
| Publisher | PANGAEA - Data Publisher for Earth & Environmental Science |
| Publishing place | Bremen/Bremerhaven |
| Document type | Book ; Online |
| Note | This dataset is supplement to doi:10.1038/msb.2011.90 |
| DOI | 10.1594/PANGAEA.775713 |
| Database | Library catalogue of the German National Library of Science and Technology (TIB), Hannover |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
Molecular and Cellular Biology. 2013 Sept. 1, v. 33, no. 18 p.3568-3579
2013
Abstract: V(D)J recombination is initiated by the RAG endonuclease, which introduces DNA double-strand breaks ... serine-threonine kinases that orchestrate the cellular responses to DNA DSBs. During V(D)J recombination, ATM and DNA-PKcs ...
| Abstract | V(D)J recombination is initiated by the RAG endonuclease, which introduces DNA double-strand breaks (DSBs) at the border between two recombining gene segments, generating two hairpin-sealed coding ends and two blunt signal ends. ATM and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) are serine-threonine kinases that orchestrate the cellular responses to DNA DSBs. During V(D)J recombination, ATM and DNA-PKcs have unique functions in the repair of coding DNA ends. ATM deficiency leads to instability of postcleavage complexes and the loss of coding ends from these complexes. DNA-PKcs deficiency leads to a nearly complete block in coding join formation, as DNA-PKcs is required to activate Artemis, the endonuclease that opens hairpin-sealed coding ends. In contrast to loss of DNA-PKcs protein, here we show that inhibition of DNA-PKcs kinase activity has no effect on coding join formation when ATM is present and its kinase activity is intact. The ability of ATM to compensate for DNA-PKcs kinase activity depends on the integrity of three threonines in DNA-PKcs that are phosphorylation targets of ATM, suggesting that ATM can modulate DNA-PKcs activity through direct phosphorylation of DNA-PKcs. Mutation of these threonine residues to alanine (DNA-PKcs³ᴬ) renders DNA-PKcs dependent on its intrinsic kinase activity during coding end joining, at a step downstream of opening hairpin-sealed coding ends. Thus, DNA-PKcs has critical functions in coding end joining beyond promoting Artemis endonuclease activity, and these functions can be regulated redundantly by the kinase activity of either ATM or DNA-PKcs. |
|---|---|
| Keywords | DNA ; alanine ; cell biology ; genes ; mutation ; phosphorylation ; protein kinases ; protein subunits ; threonine |
| Language | English |
| Dates of publication | 2013-0901 |
| Size | p. 3568-3579. |
| Publishing place | Taylor & Francis |
| Document type | Article ; Online |
| ZDB-ID | 779397-2 |
| ISSN | 1098-5549 ; 0270-7306 |
| ISSN (online) | 1098-5549 |
| ISSN | 0270-7306 |
| DOI | 10.1128/MCB.00308-13 |
| Database | NAL-Catalogue (AGRICOLA) |
| Zs.A 1666: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
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The Journal of experimental medicine
2004 Volume 199, Issue 4, Page(s) 491–502
Abstract: Expression of V(D)J recombinase activity in developing lymphocytes is absolutely required ... for initiation of V(D)J recombination at antigen receptor loci. However, little is known about when during ... hematopoietic development the V(D)J recombinase is first active, nor is it known what elements activate ...
| Abstract | Expression of V(D)J recombinase activity in developing lymphocytes is absolutely required for initiation of V(D)J recombination at antigen receptor loci. However, little is known about when during hematopoietic development the V(D)J recombinase is first active, nor is it known what elements activate the recombinase in multipotent hematopoietic progenitors. Using mice that express a fluorescent transgenic V(D)J recombination reporter, we show that the V(D)J recombinase is active as early as common lymphoid progenitors (CLPs) but not in the upstream progenitors that retain myeloid lineage potential. Evidence of this recombinase activity is detectable in all four progeny lineages (B, T, and NK, and DC), and rag2 levels are the highest in progenitor subsets immediately downstream of the CLP. By single cell PCR, we demonstrate that V(D)J rearrangements are detectable at IgH loci in approximately 5% of splenic natural killer cells. Finally, we show that recombinase activity in CLPs is largely controlled by the Erag enhancer. As activity of the Erag enhancer is restricted to the B cell lineage, this provides the first molecular evidence for establishment of a lineage-specific transcription program in multipotent progenitors. |
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| MeSH term(s) | Animals ; B-Lymphocytes/enzymology ; B-Lymphocytes/immunology ; Green Fluorescent Proteins ; Hematopoietic Stem Cells/enzymology ; Hematopoietic Stem Cells/immunology ; Homeodomain Proteins/genetics ; Luminescent Proteins/genetics ; Lymphopoiesis/immunology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mice, Transgenic ; Polymerase Chain Reaction ; VDJ Recombinases/genetics ; VDJ Recombinases/metabolism |
| Chemical Substances | Homeodomain Proteins ; Luminescent Proteins ; RAG-1 protein (128559-51-3) ; Green Fluorescent Proteins (147336-22-9) ; VDJ Recombinases (EC 2.7.7.-) |
| Language | English |
| Publishing date | 2004-02-16 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, U.S. Gov't, P.H.S. |
| ZDB-ID | 218343-2 |
| ISSN | 1540-9538 ; 0022-1007 |
| ISSN (online) | 1540-9538 |
| ISSN | 0022-1007 |
| DOI | 10.1084/jem.20031800 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Ua VI Zs.107: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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| Zs.MG 45: Show issues |
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