LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 155

Search options

  1. Article: A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool.

    Schiller, Annemarie / Zhang, Ting / Li, Ruliang / Duechting, Andrea / Sundararaman, Srividya / Przybyla, Anna / Kuerten, Stefanie / Lehmann, Paul V

    Cells

    2017  Volume 6, Issue 4

    Abstract: Testing of peripheral blood mononuclear cells (PBMC) for immune monitoring purposes requires verification of their functionality. This is of particular concern when the PBMC have been shipped or stored for prolonged periods of time. While the CEF ( ... ...

    Abstract Testing of peripheral blood mononuclear cells (PBMC) for immune monitoring purposes requires verification of their functionality. This is of particular concern when the PBMC have been shipped or stored for prolonged periods of time. While the CEF (Cytomegalo-, Epstein-Barr and Flu-virus) peptide pool has become the gold standard for testing CD8 cell functionality, a positive control for CD4 cells is so far lacking. The latter ideally consists of proteins so as to control for the functionality of the antigen processing and presentation compartments, as well. Aiming to generate a positive control for CD4 cells, we first selected 12 protein antigens from infectious/environmental organisms that are ubiquitous: Varicella, Influenza, Parainfluenza, Mumps, Cytomegalovirus,
    Language English
    Publishing date 2017-12-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2661518-6
    ISSN 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells6040047
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Health systems preparedness for COVID-19 pandemic.

    Sundararaman, T

    Indian journal of public health

    2020  Volume 64, Issue Supplement, Page(s) S91–S93

    Abstract: Some nations in the world and some states in India have had more success in containing this pandemic. Recent efforts in strengthening the health sector have focused largely on reforms in modes of financing, but as the pandemic brings home to us, the main ...

    Abstract Some nations in the world and some states in India have had more success in containing this pandemic. Recent efforts in strengthening the health sector have focused largely on reforms in modes of financing, but as the pandemic brings home to us, the main challenge in India remains the challenge of the organization of public services using a health systems understanding. A close to community comprehensive primary health care, quality assurance, and planned excess capacity in public health systems, a more robust disease surveillance systems that can integrate data on new outbreaks and the indigenous technological capacity to scale up innovation and manufacture of essential health commodities are some of our most important requirements for both epidemic preparedness and response.
    MeSH term(s) Betacoronavirus ; COVID-19 ; Capacity Building ; Community Health Services/organization & administration ; Coronavirus Infections/epidemiology ; Disaster Planning/organization & administration ; Humans ; India/epidemiology ; Pandemics ; Pneumonia, Viral/epidemiology ; Primary Health Care/organization & administration ; Public Health Administration ; Public Health Surveillance ; Quality Assurance, Health Care/organization & administration ; SARS-CoV-2 ; Social Determinants of Health
    Keywords covid19
    Language English
    Publishing date 2020-06-10
    Publishing country India
    Document type Journal Article
    ZDB-ID 800737-8
    ISSN 2229-7693 ; 0019-557X
    ISSN (online) 2229-7693
    ISSN 0019-557X
    DOI 10.4103/ijph.IJPH_507_20
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 760: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: A Positive Control for Detection of Functional CD4 T Cells in PBMC

    Annemarie Schiller / Ting Zhang / Ruliang Li / Andrea Duechting / Srividya Sundararaman / Anna Przybyla / Stefanie Kuerten / Paul V. Lehmann

    Cells, Vol 6, Iss 4, p

    The CPI Pool

    2017  Volume 47

    Abstract: Testing of peripheral blood mononuclear cells (PBMC) for immune monitoring purposes requires verification of their functionality. This is of particular concern when the PBMC have been shipped or stored for prolonged periods of time. While the CEF ( ... ...

    Abstract Testing of peripheral blood mononuclear cells (PBMC) for immune monitoring purposes requires verification of their functionality. This is of particular concern when the PBMC have been shipped or stored for prolonged periods of time. While the CEF (Cytomegalo-, Epstein-Barr and Flu-virus) peptide pool has become the gold standard for testing CD8 cell functionality, a positive control for CD4 cells is so far lacking. The latter ideally consists of proteins so as to control for the functionality of the antigen processing and presentation compartments, as well. Aiming to generate a positive control for CD4 cells, we first selected 12 protein antigens from infectious/environmental organisms that are ubiquitous: Varicella, Influenza, Parainfluenza, Mumps, Cytomegalovirus, Streptococcus, Mycoplasma, Lactobacillus, Neisseria, Candida, Rubella, and Measles. Of these antigens, three were found to elicited interferon (IFN)-γ-producing CD4 cells in the majority of human test subjects: inactivated cytomegalo-, parainfluenza-, and influenza virions (CPI). While individually none of these three antigens triggered a recall response in all donors, the pool of the three (the ‘CPI pool’), did. One hundred percent of 245 human donors tested were found to be CPI positive, including Caucasians, Asians, and African-Americans. Therefore, the CPI pool appears to be suitable to serve as universal positive control for verifying the functionality of CD4 and of antigen presenting cells.
    Keywords PBMC quality assessment ; PBMC cryopreservation ; CD4 cell function ; ELISPOT ; ImmunoSpot ; immune monitoring ; Biology (General) ; QH301-705.5
    Subject code 570
    Language English
    Publishing date 2017-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity.

    Wunsch, Marie / Zhang, Wenji / Hanson, Jodi / Caspell, Richard / Karulin, Alexey Y / Recks, Mascha S / Kuerten, Stefanie / Sundararaman, Srividya / Lehmann, Paul V

    Viruses

    2015  Volume 7, Issue 8, Page(s) 4414–4437

    Abstract: ... information is available on the contribution of CD8 T cells and antibodies to anti-HCMV immunity, studies ... of the TH1, TH2, and TH17 subsets have been limited by the low frequency of HCMV-specific CD4 T ...

    Abstract Most humans become infected with human cytomegalovirus (HCMV). Typically, the immune system controls the infection, but the virus persists and can reactivate in states of immunodeficiency. While substantial information is available on the contribution of CD8 T cells and antibodies to anti-HCMV immunity, studies of the TH1, TH2, and TH17 subsets have been limited by the low frequency of HCMV-specific CD4 T cells in peripheral blood mononuclear cell (PBMC). Using the enzyme-linked Immunospotr assay (ELISPOT) that excels in low frequency measurements, we have established these in a sizable cohort of healthy HCMV controllers. Cytokine recall responses were seen in all seropositive donors. Specifically, interferon (IFN)- and/or interleukin (IL)-17 were seen in isolation or with IL-4 in all test subjects. IL-4 recall did not occur in isolation. While the ratios of TH1, TH2, and TH17 cells exhibited substantial variations between different individuals these ratios and the frequencies were relatively stable when tested in samples drawn up to five years apart. IFN- and IL-2 co-expressing polyfunctional cells were seen in most subjects. Around half of the HCMV-specific CD4 cells were in a reversible state of exhaustion. The data provided here established the TH1, TH2, and TH17 characteristic of the CD4 cells that convey immune protection for successful immune surveillance against which reactivity can be compared when the immune surveillance of HCMV fails.
    MeSH term(s) CD4-Positive T-Lymphocytes/immunology ; Carrier State/immunology ; Carrier State/virology ; Cytomegalovirus/immunology ; Cytomegalovirus Infections/immunology ; Cytomegalovirus Infections/virology ; Enzyme-Linked Immunospot Assay ; Healthy Volunteers ; Humans ; Interferon-gamma/secretion ; Interleukin-17/secretion ; Interleukin-4/secretion ; T-Lymphocyte Subsets/immunology ; Th1 Cells/immunology ; Th17 Cells/immunology ; Th2 Cells/immunology ; Virus Latency
    Chemical Substances IL4 protein, human ; Interleukin-17 ; Interleukin-4 (207137-56-2) ; Interferon-gamma (82115-62-6)
    Language English
    Publishing date 2015-08-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v7082828
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Book ; Online ; E-Book: Public health informatics

    Sahay, Sundeep / Sundararaman, T. / Braa, Jørn

    designing for change - a developing country perspective

    2017  

    Author's details Sundeep Sahay, T. Sundararaman, Jørn Braa
    Keywords Medical informatics ; Public health
    Subject code 610.285
    Language English
    Size 1 Online-Ressource (xviii, 270 Seiten), Illustrationen
    Publisher Oxford University Press
    Publishing place Oxford
    Publishing country Great Britain
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT019284112
    ISBN 978-0-19-107653-4 ; 9780198758778 ; 0-19-107653-8 ; 0198758774
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    Full text online

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article: Serial Measurements of Apoptotic Cell Numbers Provide Better Acceptance Criterion for PBMC Quality than a Single Measurement Prior to the T Cell Assay.

    Wunsch, Marie / Caspell, Richard / Kuerten, Stefanie / Lehmann, Paul V / Sundararaman, Srividya

    Cells

    2015  Volume 4, Issue 1, Page(s) 40–55

    Abstract: ... while promoting phagocytosis. Increased numbers of apoptotic cells in PBMC may modulate T cell functions ... in antigen-triggered T cell assays. We assessed the effect of apoptotic bystander cells on a T cell ELISPOT ... of apoptotic B cells did not affect T cell functionality. In contrast, when PBMC were stored under unfavorable ...

    Abstract As soon as Peripheral Blood Mononuclear Cells (PBMC) are isolated from whole blood, some cells begin dying. The rate of apoptotic cell death is increased when PBMC are shipped, cryopreserved, or stored under suboptimal conditions. Apoptotic cells secrete cytokines that suppress inflammation while promoting phagocytosis. Increased numbers of apoptotic cells in PBMC may modulate T cell functions in antigen-triggered T cell assays. We assessed the effect of apoptotic bystander cells on a T cell ELISPOT assay by selectively inducing B cell apoptosis using α-CD20 mAbs. The presence of large numbers of apoptotic B cells did not affect T cell functionality. In contrast, when PBMC were stored under unfavorable conditions, leading to damage and apoptosis in the T cells as well as bystander cells, T cell functionality was greatly impaired. We observed that measuring the number of apoptotic cells before plating the PBMC into an ELISPOT assay did not reflect the extent of PBMC injury, but measuring apoptotic cell frequencies at the end of the assay did. Our data suggest that measuring the numbers of apoptotic cells prior to and post T cell assays may provide more stringent PBMC quality acceptance criteria than measurements done only prior to the start of the assay.
    Language English
    Publishing date 2015-01-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2661518-6
    ISSN 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells4010040
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Health systems preparedness for COVID-19 pandemic

    Sundararaman, T

    Indian Journal of Public Health

    2020  Volume 64, Issue 6, Page(s) 91

    Keywords General Medicine ; covid19
    Language English
    Publisher Medknow
    Publishing country in
    Document type Article ; Online
    ZDB-ID 800737-8
    ISSN 2229-7693 ; 0019-557X
    ISSN (online) 2229-7693
    ISSN 0019-557X
    DOI 10.4103/ijph.ijph_507_20
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 760: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article: Health systems preparedness for COVID-19 pandemic

    Sundararaman, T

    Indian J Public Health

    Abstract: Some nations in the world and some states in India have had more success in containing this pandemic. Recent efforts in strengthening the health sector have focused largely on reforms in modes of financing, but as the pandemic brings home to us, the main ...

    Abstract Some nations in the world and some states in India have had more success in containing this pandemic. Recent efforts in strengthening the health sector have focused largely on reforms in modes of financing, but as the pandemic brings home to us, the main challenge in India remains the challenge of the organization of public services using a health systems understanding. A close to community comprehensive primary health care, quality assurance, and planned excess capacity in public health systems, a more robust disease surveillance systems that can integrate data on new outbreaks and the indigenous technological capacity to scale up innovation and manufacture of essential health commodities are some of our most important requirements for both epidemic preparedness and response.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #32496232
    Database COVID19

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article ; Online: Health systems preparedness for COVID-19 pandemic

    T Sundararaman

    Indian Journal of Public Health, Vol 64, Iss 6, Pp 91-

    2020  Volume 93

    Abstract: Some nations in the world and some states in India have had more success in containing this pandemic. Recent efforts in strengthening the health sector have focused largely on reforms in modes of financing, but as the pandemic brings home to us, the main ...

    Abstract Some nations in the world and some states in India have had more success in containing this pandemic. Recent efforts in strengthening the health sector have focused largely on reforms in modes of financing, but as the pandemic brings home to us, the main challenge in India remains the challenge of the organization of public services using a health systems understanding. A close to community comprehensive primary health care, quality assurance, and planned excess capacity in public health systems, a more robust disease surveillance systems that can integrate data on new outbreaks and the indigenous technological capacity to scale up innovation and manufacture of essential health commodities are some of our most important requirements for both epidemic preparedness and response.
    Keywords covid-19 ; comprehensive primary health care ; health systems approach ; health systems preparedness ; Public aspects of medicine ; RA1-1270 ; covid19
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Wolters Kluwer Medknow Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article ; Online: National Health Policy 2017: a cautious welcome.

    Sundararaman, T

    Indian journal of medical ethics

    2017  Volume 2, Issue 2, Page(s) 69–71

    Abstract: On March 15, 2017 the union cabinet approved the new National Health Policy. The next day a 28-page policy text and an accompanying 13-page situational analysis were placed in Parliament and in the public domain. To have, at all times, a health policy in ...

    Abstract On March 15, 2017 the union cabinet approved the new National Health Policy. The next day a 28-page policy text and an accompanying 13-page situational analysis were placed in Parliament and in the public domain. To have, at all times, a health policy in place that shows a road map on how a nation would show "progressive realization" of health as a basic human right is an obligation under the International Covenant on Economic, Social and Cultural Rights. This is an international treaty adopted in 1976, to which India became a signatory in 1979, and this was one of the catalysts for the adoption of the first National Health Policy in 1983. The immediate political backdrop to the articulation of a National Health Policy 2017 (NHP 2017), replacing the 2002 policy, is that a new health policy and a national health assurance plan were both part of the BJP's electoral manifesto. It has taken close to 34 months after the government took office, and some 26 months after the draft was circulated for public discussion, to finally approve the policy. This is reflective of the considerable contestation and contradictory pressures, often almost evenly matched, that went into finalising this policy.
    MeSH term(s) Health Policy ; Human Rights/legislation & jurisprudence ; Humans ; India ; Politics ; Public Health/legislation & jurisprudence ; Public Policy/legislation & jurisprudence
    Language English
    Publishing date 2017-04-04
    Publishing country India
    Document type Editorial
    ISSN 0975-5691
    ISSN (online) 0975-5691
    DOI 10.20529/ijme.2017.018
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top