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Article ; Online: Influence of Keratinized Tissue on Short Dental Implants: A Parallel Cohort Retrospective Study on 217 Implants with a Mean Follow-up of 4.1 Years.

Felice, Pietro / Bonifazi, Lorenzo / Pistilli, Roberto / Ferri, Agnese / Gasparro, Roberta / Barausse, Carlo

The International journal of oral & maxillofacial implants

2023 Volume 38, Issue 3, Page(s) 462–467

Abstract: Purpose: To assess whether the presence or absence of keratinized tissue height (KTh) may have an influence on marginal bone levels, complications, and implant survival for short implants.: Materials and methods: The study was designed as parallel ... ...

Abstract	Purpose: To assess whether the presence or absence of keratinized tissue height (KTh) may have an influence on marginal bone levels, complications, and implant survival for short implants. Materials and methods: The study was designed as parallel cohort retrospective research. Short implants with an implant length < 7 mm were considered. One cohort was composed of patients with short implants surrounded by ≥ 2 mm of KTh (adequate KTh); the other cohort included implants with < 2 mm of KTh (not-adequate KTh). Outcome measures were marginal bone level (MBL) changes, failures, and complications. Results: One hundred ten patients treated with 217 short and extrashort implants (4 to 6.6 mm long) were retrospectively included. The mean follow-up was 4.1 years after prosthetic loading (range: 1 to 8 years). The differences between KTh groups in MBL were not statistically significant at every follow-up considered: 0.05 mm at 1 year ( Conclusion: This study showed no statistically significant differences in MBL, complications, and implant failure rates between short implants with adequate or not-adequate KThs. However, given the importance of patient comfort while brushing and plaque accumulation, keratinized tissue grafts could be important in selected patients, especially for those who are severely atrophic, also taking into consideration all the limitations of this study and the mediumterm follow-up. Nevertheless, longer follow-ups, larger numbers of patients, and randomized controlled clinical trials are needed before making more reliable clinical recommendations. Int J Oral Maxillofac Implants 2023;38:462-467. doi: 10.11607/jomi.9918.
MeSH term(s)	Humans ; Dental Implants/adverse effects ; Dental Implantation, Endosseous/adverse effects ; Retrospective Studies ; Follow-Up Studies ; Alveolar Ridge Augmentation ; Dental Prosthesis Design ; Dental Prosthesis, Implant-Supported/adverse effects ; Dental Restoration Failure ; Treatment Outcome
Chemical Substances	Dental Implants
Language	English
Publishing date	2023-06-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	632880-5
ISSN	1942-4434 ; 0882-2786
ISSN (online)	1942-4434
ISSN	0882-2786
DOI	10.11607/jomi.9918
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Article ; Online: Combined therapy targeting AR and EZH2 curbs castration-resistant prostate cancer enhancing anti-tumor T-cell response.

Fischetti, Irene / Botti, Laura / Sulsenti, Roberta / Cancila, Valeria / Enriquez, Claudia / Ferri, Renata / Bregni, Marco / Crivelli, Filippo / Tripodo, Claudio / Colombo, Mario P / Jachetti, Elena

Epigenomics

2024

Abstract: Aim: ...

Abstract	Aim:
Language	English
Publishing date	2024-03-26
Publishing country	England
Document type	Journal Article
ZDB-ID	2537199-X
ISSN	1750-192X ; 1750-1911
ISSN (online)	1750-192X
ISSN	1750-1911
DOI	10.2217/epi-2023-0374
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Article ; Online: Proteome data of neuroblastoma cells overexpressing Neuroglobin.

Costanzo, Michele / Caterino, Marianna / Salvatori, Illari / Manganelli, Valeria / Ferri, Alberto / Misasi, Roberta / Ruoppolo, Margherita

Data in brief

2022 Volume 41, Page(s) 107843

Abstract: In this article, we present data on the proteome of human neuroblastoma cells stably overexpressing Neuroglobin (NGB). The neuroprotective role of NGB is clearly established, nevertheless the related mechanistic processes, which are dependent on NGB ... ...

Abstract	In this article, we present data on the proteome of human neuroblastoma cells stably overexpressing Neuroglobin (NGB). The neuroprotective role of NGB is clearly established, nevertheless the related mechanistic processes, which are dependent on NGB overexpression, are not known. To address this question, we performed shotgun label-free quantification (LFQ) proteomics using an SH-SY5Y cell model of neuroblastoma that overexpresses an NGB-FLAG construct, and wild type cells transfected with an empty vector as control (CTRL). The proteomes from six biological samples per condition were digested using the S-Trap sample preparation followed by LC-MS/MS analysis with a LTQ-Orbitrap XL mass spectrometer. The quantitative analysis was performed using the LFQ algorithm of MaxQuant, leading to 1654 correctly quantified proteins over 2580 identified proteins. Finally, the statistic comparison of the two analyzed groups within Perseus platform identified 178 differential proteins (107 up- and 71 down-regulated). In addition, multivariate statistical analysis was carried out using MetaboAnalyst 5.0 software. MS proteomics data are available via ProteomeXchange with the dataset identifier PXD029012.
Language	English
Publishing date	2022-01-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2786545-9
ISSN	2352-3409 ; 2352-3409
ISSN (online)	2352-3409
ISSN	2352-3409
DOI	10.1016/j.dib.2022.107843
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Article ; Online: A home-based lifestyle intervention program reduces the tumorigenic potential of triple-negative breast cancer cells.

Baldelli, Giulia / Natalucci, Valentina / Ferri Marini, Carlo / Sisti, Davide / Annibalini, Giosuè / Saltarelli, Roberta / Bocconcelli, Matteo / Gentilini, Veronica / Emili, Rita / Rocchi, Marco Bruno Luigi / Lucertini, Francesco / Barbieri, Elena / Brandi, Giorgio / De Santi, Mauro

Scientific reports

2024 Volume 14, Issue 1, Page(s) 2409

Abstract: Translational research for the evaluation of physical activity habits and lifestyle modifications based on nutrition and exercise has recently gained attention. In this study, we evaluated the effects of serum samples obtained before and after a 12-week ... ...

Abstract	Translational research for the evaluation of physical activity habits and lifestyle modifications based on nutrition and exercise has recently gained attention. In this study, we evaluated the effects of serum samples obtained before and after a 12-week home-based lifestyle intervention based on nutrition and exercise in breast cancer survivors in terms of modulation of the tumorigenic potential of breast cancer cells. The home-based lifestyle intervention proposed in this work consisted of educational counselling on exercise and nutritional behaviors and in 12 weeks of structured home-based exercise. Triple-negative breast cancer cell line MDA-MB-231 was cultured in semi-solid medium (3D culture) with sera collected before (PRE) and after (POST) the lifestyle intervention program. Spheroid formation was evaluated by counting cell colonies after 3 weeks of incubation. Results show a slight but significant reduction of spheroid formation induced by serum collected POST in comparison to those obtained PRE. Moreover, statistical analyses aimed to find physiologic and metabolic parameters associated with 3D cell proliferation revealed the proliferative inducer IGF-1 as the only predictor of cell tumorigenic potential. These results highlight the importance of lifestyle changes for cancer progression control in a tertiary prevention context. Translational research could offer a useful tool to identify metabolic and physiological changes induced by exercise and nutritional behaviors associated with cancer progression and recurrence risk.
MeSH term(s)	Humans ; Female ; Triple Negative Breast Neoplasms/prevention & control ; Breast Neoplasms/prevention & control ; Life Style ; Health Behavior ; Exercise/physiology ; Carcinogenesis ; Counseling
Language	English
Publishing date	2024-01-29
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-52065-9
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Article ; Online: Anti-CFH-associated hemolytic uremic syndrome: do we still need plasma exchange?

Ferri, Marion / Zotta, Federica / Donadelli, Roberta / Dossier, Claire / Duneton, Charlotte / El-Sissy, Carine / Fremeau-Bacchi, Véronique / Kwon, Thérésa / Quadri, Lisa / Pasini, Andrea / Sellier-Leclerc, Anne-Laure / Vivarelli, Marina / Hogan, Julien

Pediatric nephrology (Berlin, Germany)

2024

Abstract: Background: Between 5 and 50% of atypical hemolytic uremic syndrome (aHUS) cases in children are caused by autoantibodies against complement factor H (CFH). Given the acquired autoimmune nature of the disease, plasma exchange (PE) and various ... ...

Abstract	Background: Between 5 and 50% of atypical hemolytic uremic syndrome (aHUS) cases in children are caused by autoantibodies against complement factor H (CFH). Given the acquired autoimmune nature of the disease, plasma exchange (PE) and various immunosuppressive treatments have been used. More recently, eculizumab has been proposed. Methods: In this multicenter, retrospective study, we report outcomes of 12 children with anti-FH antibody-associated HUS treated with eculizumab associated with various immunosuppressive regimens. Results: Patients were treated with eculizumab for 15.5 [9.5;23.0] months and 3 received PE or IgG adsorption. Three patients received mycophenolate mofetil (MMF) alone, 1 patient received MMF and steroids, 1 patient received MMF and rituximab, 3 patients received MMF/steroids and rituximab, and 4 patients did not receive any immunosuppression. Anti-FH antibody levels significantly decreased but no difference was observed based on the immunosuppressive regimen. Eculizumab was discontinued in 7/10 patients after 11 [7.5;15.5] months and MMF in 6/8 patients after 36 [35;40] months. Anti-FH titers at MMF discontinuation ranged from 257 to 3425 UI/L. None of these patients relapsed and eGFR at last follow-up was above 70 mL/min/1.73 m Conclusions: Eculizumab is effective and safe in inducing and maintaining remission in aHUS secondary to anti-FH antibodies and renders reduction of anti-FH titers less urgent. Anti-FH antibody titers decreased in most patients irrespective of the immunosuppressive treatment chosen, so that a strategy consisting of combining eculizumab with MMF monotherapy seems sufficient at least in non-Indian or less severe forms of anti-FH antibody-associated HUS.
Language	English
Publishing date	2024-04-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	631932-4
ISSN	1432-198X ; 0931-041X
ISSN (online)	1432-198X
ISSN	0931-041X
DOI	10.1007/s00467-024-06373-x
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Article ; Online: Proteome data of neuroblastoma cells overexpressing Neuroglobin

Michele Costanzo / Marianna Caterino / Illari Salvatori / Valeria Manganelli / Alberto Ferri / Roberta Misasi / Margherita Ruoppolo

Data in Brief, Vol 41, Iss , Pp 107843- (2022)

2022

Abstract	In this article, we present data on the proteome of human neuroblastoma cells stably overexpressing Neuroglobin (NGB). The neuroprotective role of NGB is clearly established, nevertheless the related mechanistic processes, which are dependent on NGB overexpression, are not known. To address this question, we performed shotgun label-free quantification (LFQ) proteomics using an SH-SY5Y cell model of neuroblastoma that overexpresses an NGB-FLAG construct, and wild type cells transfected with an empty vector as control (CTRL). The proteomes from six biological samples per condition were digested using the S-Trap sample preparation followed by LC-MS/MS analysis with a LTQ-Orbitrap XL mass spectrometer. The quantitative analysis was performed using the LFQ algorithm of MaxQuant, leading to 1654 correctly quantified proteins over 2580 identified proteins. Finally, the statistic comparison of the two analyzed groups within Perseus platform identified 178 differential proteins (107 up- and 71 down-regulated). In addition, multivariate statistical analysis was carried out using MetaboAnalyst 5.0 software. MS proteomics data are available via ProteomeXchange with the dataset identifier PXD029012.
Keywords	Proteomics ; Neuroglobin ; Label-free quantification ; LC-MS/MS ; S-Trap ; MaxQuant ; Computer applications to medicine. Medical informatics ; R858-859.7 ; Science (General) ; Q1-390
Subject code	310
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article: Proteome data of neuroblastoma cells overexpressing Neuroglobin

Costanzo, Michele / Caterino, Marianna / Salvatori, Illari / Manganelli, Valeria / Ferri, Alberto / Misasi, Roberta / Ruoppolo, Margherita

Data in Brief. 2022 Apr., v. 41

2022

Abstract	In this article, we present data on the proteome of human neuroblastoma cells stably overexpressing Neuroglobin (NGB). The neuroprotective role of NGB is clearly established, nevertheless the related mechanistic processes, which are dependent on NGB overexpression, are not known. To address this question, we performed shotgun label-free quantification (LFQ) proteomics using an SH-SY5Y cell model of neuroblastoma that overexpresses an NGB-FLAG construct, and wild type cells transfected with an empty vector as control (CTRL). The proteomes from six biological samples per condition were digested using the S-Trap sample preparation followed by LC-MS/MS analysis with a LTQ-Orbitrap XL mass spectrometer. The quantitative analysis was performed using the LFQ algorithm of MaxQuant, leading to 1654 correctly quantified proteins over 2580 identified proteins. Finally, the statistic comparison of the two analyzed groups within Perseus platform identified 178 differential proteins (107 up- and 71 down-regulated). In addition, multivariate statistical analysis was carried out using MetaboAnalyst 5.0 software. MS proteomics data are available via ProteomeXchange with the dataset identifier PXD029012.
Keywords	algorithms ; computer software ; data collection ; humans ; models ; multivariate analysis ; neuroprotective effect ; proteome ; proteomics ; quantitative analysis ; spectrometers
Language	English
Dates of publication	2022-04
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	2786545-9
ISSN	2352-3409
ISSN	2352-3409
DOI	10.1016/j.dib.2022.107843
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Article: Giant fibrovascular polyps of the esophagus. Trans oral versus surgical approach. Case report and systematic literature review.

Ferri, Valentina / Vicente, Emilio / Quijano, Yolanda / Duran, Hipolito / Diaz, Eduardo / Fabra, Isabel / Malave, Luis / Ruiz, Pablo / Isernia, Roberta / Caruso, Riccardo

International journal of surgery case reports

2022 Volume 97, Page(s) 107412

Abstract: Introduction: Giant fibrovascular esophageal polyp is a rare benign intraluminal tumour. The aim of this study is to perform a review of the most recent literature in order to describe and analyse the current range of possible diagnostics and treatment ... ...

Abstract	Introduction: Giant fibrovascular esophageal polyp is a rare benign intraluminal tumour. The aim of this study is to perform a review of the most recent literature in order to describe and analyse the current range of possible diagnostics and treatment strategies. Case report: We present two cases of giant fibrovascular esophageal polyp treated with a combined minimally invasive transluminal approach at Sanchinarro University Hospital. Further, we perform a literature review. Conclusion: We present two cases of grant fibrovascular polyp submitted to minimally invasive transluminal approach. Furthermore, 54 original articles reporting 59 cases have been analysed. In the surgical group, an esophagotomy and polyp resection were performed in 31 (91 %) patients and a total esophagectomy in two patients (5,8 %). Severe morbidity occurred in two patients (5,8 %.) The median hospital stay was 9.25 days. A total of two (5,8 %) cases of recurrence have been registered. In the minimally invasive transluminal approach group, 27 patients had a polyp resection performed completely by endoscopy/transoral. There were no complications but there was one case of recurrence. Conclusion: The transluminal approach is safe and should be considered also in the treatment of large esophageal polyps.
Language	English
Publishing date	2022-07-13
Publishing country	Netherlands
Document type	Journal Article
ISSN	2210-2612
ISSN	2210-2612
DOI	10.1016/j.ijscr.2022.107412
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Article: Differences in CSF Biomarkers Profile of Patients with Parkinson's Disease Treated with MAO-B Inhibitors in Add-On.

Zenuni, Henri / Candelise, Niccolò / Grillo, Piergiorgio / Simonetta, Clara / Bovenzi, Roberta / Ferri, Alberto / Valle, Cristiana / Mercuri, Nicola Biagio / Schirinzi, Tommaso

Journal of integrative neuroscience

2022 Volume 21, Issue 6, Page(s) 165

Abstract: Background: Monoamine oxidase type B inhibitors (iMAO-Bs) are a class of largely-used antiparkinsonian agents that, based on experimental evidence, are supposed to exert different degrees of neuroprotection in Parkinson's disease (PD). However, clinical ...

Abstract	Background: Monoamine oxidase type B inhibitors (iMAO-Bs) are a class of largely-used antiparkinsonian agents that, based on experimental evidence, are supposed to exert different degrees of neuroprotection in Parkinson's disease (PD). However, clinical proofs on this regard are very scarce. Since cerebrospinal fluid (CSF) reflects pathological changes occurring at brain level, we examined the neurodegeneration-related CSF biomarkers profile of PD patients under chronic treatment with different iMAO-Bs to identify biochemical signatures suggestive for differential neurobiological effects. Methods: Thirty-five PD patients under chronic treatment with different iMAO-Bs in add-on to levodopa were enrolled and grouped in rasagiline (n = 13), selegiline (n = 9), safinamide (n = 13). Respective standard clinical scores for motor and non-motor disturbances, together with CSF biomarkers of neurodegeneration levels (amyloid- β -42, amyloid- β -40, total and 181-phosphorylated tau, and lactate) were collected and compared among the three iMAO-B groups. Results: No significant clinical differences emerged among the iMAO-B groups. CSF levels of tau proteins and lactate were instead different, resulting higher in patients under selegiline than in those under rasagiline and safinamide. Conclusions: Although preliminary and limited, this study indicates that patients under different iMAO-Bs may present distinct profiles of CSF neurodegeneration-related biomarkers, probably because of the differential neurobiological effects of the drugs. Larger studies are now needed to confirm and extend these initial observations.
MeSH term(s)	Humans ; Biomarkers ; Lactates ; Parkinson Disease/drug therapy ; Selegiline/therapeutic use ; Monoamine Oxidase Inhibitors/therapeutic use
Chemical Substances	Biomarkers ; Lactates ; rasagiline (003N66TS6T) ; safinamide (90ENL74SIG) ; Selegiline (2K1V7GP655) ; Monoamine Oxidase Inhibitors
Language	English
Publishing date	2022-11-24
Publishing country	Singapore
Document type	Journal Article
ZDB-ID	2136427-8
ISSN	0219-6352
ISSN	0219-6352
DOI	10.31083/j.jin2106165
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Article ; Online: NGS study in a sicilian case series with a genetic diagnosis for Gerstmann-Sträussler-Scheinker syndrome (PRNP, p.P102L).

Salemi, Michele / Mandarà, Luana G M / Salluzzo, Maria Grazia / Schillaci, Francesca A / Castiglione, Roberto / Cordella, Angela / Iorio, Roberta / Perrotta, Concetta Simona / Ferri, Raffaele / Romano, Corrado

Molecular biology reports

2023 Volume 50, Issue 11, Page(s) 9715–9720

Abstract: Background: Gerstmann Sträussler Scheinker (GSS) is an inherited, invariably fatal prion disease. Like other human prion diseases, GSS is caused by missense mutations in the prion protein (PrP) gene (PRNP), and by the formation and overtime accumulation ...

Abstract	Background: Gerstmann Sträussler Scheinker (GSS) is an inherited, invariably fatal prion disease. Like other human prion diseases, GSS is caused by missense mutations in the prion protein (PrP) gene (PRNP), and by the formation and overtime accumulation of the misfolded, pathogenic scrapie PrP (PrPSc). The first mutation identified in the PRNP gene, and the one blamed as the main cause of the disease, is c.C305T:p.P102L. Methods and results: The Sanger sequencing method was performed on the PRNP gene for the detection of c.C305T:p.P102L mutations in a cohort of 10 subjects; moreover, a study was carried out, using Next Generation Sequencing (NGS), by sequencing a group of genes related to amyotrophic lateral sclerosis (ALS), Alzheimer's disease (AD), movement disorders and dementia which show a phenotypic profile similar to that of GSS. The results obtained from the study using NGS indicate the potential role of other genetic variants which could contribute to the various GSS phenotypes. Conclusions: In conclusion, we highlight the large clinical variability in subjects presenting with GSS and p.P102L, as well as the hypothesis that the mutation in PrP codon 102 alone is not sufficient to trigger the cardinal clinical signs of the disease; furthermore, we do not exclude the possibility that further genetic variants play a decisive role in the aspects of the various phenotypes with which GSS manifests itself.
MeSH term(s)	Animals ; Humans ; Gerstmann-Straussler-Scheinker Disease/diagnosis ; Gerstmann-Straussler-Scheinker Disease/genetics ; Gerstmann-Straussler-Scheinker Disease/metabolism ; Prions/genetics ; Prion Proteins/genetics ; Mutation/genetics ; High-Throughput Nucleotide Sequencing
Chemical Substances	Prions ; Prion Proteins ; PRNP protein, human
Language	English
Publishing date	2023-10-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	186544-4
ISSN	1573-4978 ; 0301-4851
ISSN (online)	1573-4978
ISSN	0301-4851
DOI	10.1007/s11033-023-08764-z
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