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  1. Article ; Online: Synthetic Notch receptors and their applications to study cell-cell contacts in vivo.

    He, Li / Perrimon, Norbert

    Developmental cell

    2023  Volume 58, Issue 3, Page(s) 171–173

    Abstract: Physical cell-cell interaction is a fundamental mechanism that controls the development and physiology of multicellular organisms. However, methods to study cell contact within complex tissues are limited. In a recent issue of Science, Zhang et al. ... ...

    Abstract Physical cell-cell interaction is a fundamental mechanism that controls the development and physiology of multicellular organisms. However, methods to study cell contact within complex tissues are limited. In a recent issue of Science, Zhang et al. developed synthetic Notch (synNotch)-based tools that can monitor and trace cell-cell contacts in mammals.
    MeSH term(s) Animals ; Receptors, Notch ; Receptors, Artificial ; Cell Communication ; Mammals
    Chemical Substances Receptors, Notch ; Receptors, Artificial
    Language English
    Publishing date 2023-01-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2054967-2
    ISSN 1878-1551 ; 1534-5807
    ISSN (online) 1878-1551
    ISSN 1534-5807
    DOI 10.1016/j.devcel.2023.01.001
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  2. Article ; Online: Prime Editing for Precise Genome Engineering in Drosophila.

    Bosch, Justin A / Perrimon, Norbert

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2540, Page(s) 113–134

    Abstract: Editing the Drosophila genome is incredibly useful for gene functional analysis. However, compared to gene knockouts, precise gene editing is difficult to achieve. Prime editing, a recently described CRISPR/Cas9-based technique, has the potential to make ...

    Abstract Editing the Drosophila genome is incredibly useful for gene functional analysis. However, compared to gene knockouts, precise gene editing is difficult to achieve. Prime editing, a recently described CRISPR/Cas9-based technique, has the potential to make precise editing simpler and faster, and produce less errors than traditional methods. Initially described in mammalian cells, prime editing is functional in Drosophila somatic and germ cells. Here, we outline steps to design, generate, and express prime editing components in transgenic flies. Furthermore, we highlight a crossing scheme to produce edited fly stocks in less than 3 months.
    MeSH term(s) Animals ; Animals, Genetically Modified ; CRISPR-Cas Systems/genetics ; Drosophila/genetics ; Gene Editing/methods ; Genome, Insect ; Mammals/genetics
    Language English
    Publishing date 2022-08-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2541-5_5
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  3. Article ; Online: No sugar, just protein please - says the fly.

    Petsakou, Afroditi / Perrimon, Norbert

    Nature metabolism

    2022  Volume 4, Issue 11, Page(s) 1436–1437

    MeSH term(s) Sugars ; Proteins ; Diptera ; Animals
    Chemical Substances Sugars ; Proteins
    Language English
    Publishing date 2022-11-04
    Publishing country Germany
    Document type Journal Article
    ISSN 2522-5812
    ISSN (online) 2522-5812
    DOI 10.1038/s42255-022-00665-y
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  4. Article ; Online: Bioelectric regulation of intestinal stem cells.

    Petsakou, Afroditi / Perrimon, Norbert

    Trends in cell biology

    2022  Volume 33, Issue 7, Page(s) 555–567

    Abstract: Proper regulation of ion balance across the intestinal epithelium is essential for physiological functions, while ion imbalance causes intestinal disorders with dire health consequences. Ion channels, pumps, and exchangers are vital for regulating ion ... ...

    Abstract Proper regulation of ion balance across the intestinal epithelium is essential for physiological functions, while ion imbalance causes intestinal disorders with dire health consequences. Ion channels, pumps, and exchangers are vital for regulating ion movements (i.e., bioelectric currents) that control epithelial absorption and secretion. Recent in vivo studies used the Drosophila gut to identify conserved pathways that link regulators of Ca
    MeSH term(s) Animals ; Drosophila/metabolism ; Stem Cells/metabolism ; Intestinal Mucosa/metabolism ; Drosophila Proteins/metabolism ; Ion Channels/metabolism ; Cell Proliferation ; Intestines
    Chemical Substances Drosophila Proteins ; Ion Channels
    Language English
    Publishing date 2022-11-15
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 30122-x
    ISSN 1879-3088 ; 0962-8924
    ISSN (online) 1879-3088
    ISSN 0962-8924
    DOI 10.1016/j.tcb.2022.10.003
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  5. Article: Sensing of dietary amino acids and regulation of calcium dynamics in adipose tissues through Adipokinetic hormone in

    Ahmad, Muhammad / Wu, Shang / Guo, Xuan / Perrimon, Norbert / He, Li

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Nutrient sensing and the subsequent metabolic responses are fundamental functions of animals, closely linked to diseases such as type 2 diabetes and various obesity-related diseases. ...

    Abstract Nutrient sensing and the subsequent metabolic responses are fundamental functions of animals, closely linked to diseases such as type 2 diabetes and various obesity-related diseases.
    Language English
    Publishing date 2024-03-08
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.03.04.583442
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  6. Article ; Online: What fuels the fly: Energy metabolism in

    Chatterjee, Nirmalya / Perrimon, Norbert

    Science advances

    2021  Volume 7, Issue 24

    Abstract: The organs and metabolic pathways involved in energy metabolism, and the process of ATP production from nutrients, are comparable between humans ... ...

    Abstract The organs and metabolic pathways involved in energy metabolism, and the process of ATP production from nutrients, are comparable between humans and
    MeSH term(s) Animals ; Diabetes Mellitus/etiology ; Drosophila/metabolism ; Drosophila melanogaster/genetics ; Energy Metabolism/genetics ; Mammals ; Obesity/genetics
    Language English
    Publishing date 2021-06-09
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.abg4336
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  7. Article ; Online: Preparation of

    Tattikota, Sudhir Gopal / Perrimon, Norbert

    Bio-protocol

    2021  Volume 11, Issue 16, Page(s) e4127

    Abstract: Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain high-quality ... ...

    Abstract Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain high-quality single cells in suspension. Recently, we used this approach to characterize
    Language English
    Publishing date 2021-08-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.4127
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  8. Article ; Online: An evolutionary mechanism to assimilate new nutrient sensors into the mTORC1 pathway.

    Liu, Grace Y / Jouandin, Patrick / Bahng, Raymond E / Perrimon, Norbert / Sabatini, David M

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 2517

    Abstract: Animals sense and respond to nutrient availability in their environments, a task coordinated in part by the mTOR complex 1 (mTORC1) pathway. mTORC1 regulates growth in response to nutrients and, in mammals, senses specific amino acids through specialized ...

    Abstract Animals sense and respond to nutrient availability in their environments, a task coordinated in part by the mTOR complex 1 (mTORC1) pathway. mTORC1 regulates growth in response to nutrients and, in mammals, senses specific amino acids through specialized sensors that bind the GATOR1/2 signaling hub. Given that animals can occupy diverse niches, we hypothesized that the pathway might evolve distinct sensors in different metazoan phyla. Whether such customization occurs, and how the mTORC1 pathway might capture new inputs, is unknown. Here, we identify the Drosophila melanogaster protein Unmet expectations (CG11596) as a species-restricted methionine sensor that directly binds the fly GATOR2 complex in a fashion antagonized by S-adenosylmethionine (SAM). We find that in Dipterans GATOR2 rapidly evolved the capacity to bind Unmet and to thereby repurpose a previously independent methyltransferase as a SAM sensor. Thus, the modular architecture of the mTORC1 pathway allows it to co-opt preexisting enzymes to expand its nutrient sensing capabilities, revealing a mechanism for conferring evolvability on an otherwise conserved system.
    MeSH term(s) Animals ; TOR Serine-Threonine Kinases/metabolism ; Drosophila melanogaster/metabolism ; Multiprotein Complexes/metabolism ; Mechanistic Target of Rapamycin Complex 1/metabolism ; S-Adenosylmethionine ; Nutrients ; Mammals/metabolism
    Chemical Substances TOR Serine-Threonine Kinases (EC 2.7.11.1) ; Multiprotein Complexes ; Mechanistic Target of Rapamycin Complex 1 (EC 2.7.11.1) ; S-Adenosylmethionine (7LP2MPO46S)
    Language English
    Publishing date 2024-03-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-46680-3
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  9. Article ; Online: The endoribonuclease Arlr is required to maintain lipid homeostasis by downregulating lipolytic genes during aging.

    Sun, Xiaowei / Shen, Jie / Perrimon, Norbert / Kong, Xue / Wang, Dan

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 6254

    Abstract: While disorders in lipid metabolism have been associated with aging and age-related diseases, how lipid metabolism is regulated during aging is poorly understood. Here, we characterize the Drosophila endoribonuclease CG2145, an ortholog of mammalian ... ...

    Abstract While disorders in lipid metabolism have been associated with aging and age-related diseases, how lipid metabolism is regulated during aging is poorly understood. Here, we characterize the Drosophila endoribonuclease CG2145, an ortholog of mammalian EndoU that we named Age-related lipid regulator (Arlr), as a regulator of lipid homeostasis during aging. In adult adipose tissues, Arlr is necessary for maintenance of lipid storage in lipid droplets (LDs) as flies age, a phenotype that can be rescued by either high-fat or high-glucose diet. Interestingly, RNA-seq of arlr mutant adipose tissues and RIP-seq suggest that Arlr affects lipid metabolism through the degradation of the mRNAs of lipolysis genes - a model further supported by the observation that knockdown of Lsd-1, regucalcin, yip2 or CG5162, which encode genes involved in lipolysis, rescue the LD defects of arlr mutants. In addition, we characterize DendoU as a functional paralog of Arlr and show that human ENDOU can rescue arlr mutants. Altogether, our study reveals a role of ENDOU-like endonucleases as negative regulator of lipolysis.
    MeSH term(s) Animals ; Humans ; Lipolysis/genetics ; Endoribonucleases/genetics ; Endoribonucleases/metabolism ; Uridylate-Specific Endoribonucleases/metabolism ; Lipid Metabolism/genetics ; Drosophila/genetics ; Drosophila/metabolism ; Aging/genetics ; Lipids ; Homeostasis/genetics ; Lipid Droplets/metabolism ; Mammals/metabolism
    Chemical Substances Endoribonucleases (EC 3.1.-) ; Uridylate-Specific Endoribonucleases (EC 3.1.-) ; Lipids
    Language English
    Publishing date 2023-10-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-42042-7
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  10. Article ; Online: Finding information about uncharacterized Drosophila melanogaster genes.

    Mohr, Stephanie E / Kim, Ah-Ram / Hu, Yanhui / Perrimon, Norbert

    Genetics

    2023  Volume 225, Issue 4

    Abstract: Genes that have been identified in the genome but remain uncharacterized with regards to function offer an opportunity to uncover novel biological information. Novelty is exciting but can also be a barrier. If nothing is known, how does one start ... ...

    Abstract Genes that have been identified in the genome but remain uncharacterized with regards to function offer an opportunity to uncover novel biological information. Novelty is exciting but can also be a barrier. If nothing is known, how does one start planning and executing experiments? Here, we provide a recommended information-mining workflow and a corresponding guide to accessing information about uncharacterized Drosophila melanogaster genes, such as those assigned only a systematic coding gene identifier. The available information can provide insights into where and when the gene is expressed, what the function of the gene might be, whether there are similar genes in other species, whether there are known relationships to other genes, and whether any other features have already been determined. In addition, available information about relevant reagents can inspire and facilitate experimental studies. Altogether, mining available information can help prioritize genes for further study, as well as provide starting points for experimental assays and other analyses.
    MeSH term(s) Animals ; Drosophila melanogaster/genetics ; Genome
    Language English
    Publishing date 2023-12-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2167-2
    ISSN 1943-2631 ; 0016-6731
    ISSN (online) 1943-2631
    ISSN 0016-6731
    DOI 10.1093/genetics/iyad187
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