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Article ; Online: Comparative analysis on characteristics of two activated partial thromboplastin time reagents.

Yasui, Yutaro / Ishii, Toshiaki / Tatebe, Junko / Morita, Toshisuke

2022 Volume 36, Issue 9, Page(s) e24608

Abstract: Background: For the lack of standardized activated partial thromboplastin time (APTT), it has been pointed out that there are differences in values among several reagents. Recently, we have performed a parallel measurement on two reagents, Thrombocheck ... ...

Abstract	Background: For the lack of standardized activated partial thromboplastin time (APTT), it has been pointed out that there are differences in values among several reagents. Recently, we have performed a parallel measurement on two reagents, Thrombocheck APTT-SLA and Coagpia APTT-n, and resulted with some dissociated samples. The purpose of this study is to clarify the possible factors related to ΔAPTT, the difference in measured values between the two reagents. Materials and methods: In order to clarify the factors related to ΔAPTT, multiple regression analysis was performed on 8324 samples, using clinical laboratory data of all test items requested simultaneously with APTT. To confirm the items extracted from the multiple regression analysis, the target substance was spiked to pooled plasma and measured with two APTT reagents. Additionally, by spiking phospholipids, the effect on APTT measurement system was assessed. Result: Multiple regression analysis detected albumin-globulin ratio (AGR), C-reactive protein (CRP), hematocrit, and prothrombin time as factors related to ΔAPTT (p < 0.001). Results revealed no significant differences when albumin was added to change the AGR. Whereas with the addition of CRP, prolongation of APTT was observed in Coagpia APTT-n compared to Thrombocheck APTT-SLA (p < 0.001). This prolongation was canceled by the addition of phospholipids, suggesting the interaction of CRP with phospholipids leads to the pseudo-prolongation. Conclusion: It is considered that the pseudo-prolongation of APTT is triggered by the interaction of CRP on the phospholipid in Coagpia APTT-n, which contributed to the APTT dissociation.
MeSH term(s)	Albumins ; Blood Coagulation Tests ; Humans ; Indicators and Reagents ; Partial Thromboplastin Time ; Phospholipids ; Prothrombin Time
Chemical Substances	Albumins ; Indicators and Reagents ; Phospholipids
Language	English
Publishing date	2022-07-19
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	645095-7
ISSN	1098-2825 ; 0887-8013
ISSN (online)	1098-2825
ISSN	0887-8013
DOI	10.1002/jcla.24608
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Zs.A 2471: Show issues

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Article ; Online: Urinary excretion of thyroid hormone in CKD patients: a proof-of-concept of nephrogenic hypothyroidism.

Yuasa, Rena / Muramatsu, Masaki / Saito, Akinobu / Osuka, Hiroyoshi / Morita, Toshisuke / Hamasaki, Yuko / Sakai, Ken

Renal failure

2023 Volume 45, Issue 2, Page(s) 2293224

Abstract: Purpose: Patients with chronic kidney disease (CKD) complicated by hypothyroidism exhibit a higher prevalence of urine protein than that in the general population. This study was aimed at investigating thyroid hormones and thyroid hormone-binding ... ...

Abstract	Purpose: Patients with chronic kidney disease (CKD) complicated by hypothyroidism exhibit a higher prevalence of urine protein than that in the general population. This study was aimed at investigating thyroid hormones and thyroid hormone-binding proteins excreted in urine to elucidate the urine protein-associated underlying mechanisms of hypothyroidism. Methods: Between November 2016 and August 2018, thyroid function (serum free T3 [sFT3], free T4 [sFT4], and thyroid-stimulating hormone [sTSH]), kidney function (estimated glomerular filtration rate [eGFR]), thyroid antibodies and albumin (Alb) were evaluated in 99 Japanese CKD patients with proteinuria at our outpatient clinic. A urine examination was also performed to assess the following parameters: total T3, total T4, TSH, Alb, preAlb, thyroid-binding globulin, and protein. Results: The median patient age at study recruitment was 60 years; 50 patients (50.5%) were male. The median eGFR and Alb level were 20.3 ml/min/1.73 m Conclusion: We found a significant association between hypothyroidism and NS regardless of sex and antibodies. Urinary loss of thyroid hormones must be a factor influencing hypothyroidism independent of autoimmunity.
MeSH term(s)	Humans ; Male ; Middle Aged ; Female ; Hypothyroidism/complications ; Thyroid Hormones/metabolism ; Renal Insufficiency, Chronic ; Thyrotropin ; Nephrotic Syndrome/complications
Chemical Substances	Thyroid Hormones ; Thyrotropin (9002-71-5)
Language	English
Publishing date	2023-12-12
Publishing country	England
Document type	Journal Article
ZDB-ID	632949-4
ISSN	1525-6049 ; 0886-022X
ISSN (online)	1525-6049
ISSN	0886-022X
DOI	10.1080/0886022X.2023.2293224
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Zs.A 1331: Show issues

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Article ; Online: Quantitative evaluation of insulin-induced abdominal subcutaneous dystrophic tissue using shear wave elastography.

Sato, Genki / Uchino, Hiroshi / Shimizu, Yosuke / Tatebe, Junko / Morita, Toshisuke / Hirose, Takahisa

Journal of diabetes investigation

2022 Volume 13, Issue 6, Page(s) 1004–1010

Abstract: Aims/introduction: Subcutaneous dystrophic tissue (DT) produced by insulin injection causes dysglycemia owing to inadequate absorption of insulin. However, precise techniques for measuring DT have not been established. Shear wave elastography (SWE) is ... ...

Abstract	Aims/introduction: Subcutaneous dystrophic tissue (DT) produced by insulin injection causes dysglycemia owing to inadequate absorption of insulin. However, precise techniques for measuring DT have not been established. Shear wave elastography (SWE) is an imaging technology that can quantify tissue stiffness. In this study, insulin injection-induced DT was quantified using SWE to generate whole-abdominal wall subcutaneous tissue by three-dimensional (3D) imaging in patients with type 2 diabetes who were treated with multiple insulin injections. Materials and methods: Seven patients with type 2 diabetes were recruited who received long-standing multiple insulin injections. Using SWE, the shear wave velocity (SWV) of DT and control (normal subcutaneous tissue) was measured. Furthermore, two of seven patients underwent whole-abdominal SWE examination to calculate the proportion of DT. A subcutaneous insulin tolerance test was also performed in both the DT and control tissues. Results: The SWV in DT was significantly higher than that in the control tissue (2.87 [2.66-2.98] vs 1.29 [1.23-1.44] m/s, P < 0.01). The proportion of the DT volume was 0.67% and 5.21% for two individuals from the entire abdominal subcutaneous tissue volume. The area under the curve for the subcutaneously injected insulin aspart concentration at the DT sites was lower than that of the control tissue (75.0 [52.1-111] vs 116 [86.9-152.5] hmU/L, P = 0.1). Conclusions:* SWE can be useful in quantifying abdominal subcutaneous insulin-induced DT, especially the 3D volume of insulin injection-induced DT from the entire abdominal subcutaneous tissue. This study is the first to examine the volume and distribution of abdominal subcutaneous DT using SWE.
MeSH term(s)	Diabetes Mellitus, Type 2/drug therapy ; Elasticity Imaging Techniques/methods ; Humans ; Insulin
Chemical Substances	Insulin
Language	English
Publishing date	2022-02-11
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2625840-7
ISSN	2040-1124 ; 2040-1116
ISSN (online)	2040-1124
ISSN	2040-1116
DOI	10.1111/jdi.13762
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Zs.A 6920: Show issues

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Article ; Online: Celastrol: a new therapeutic potential of traditional Chinese medicine.

Morita, Toshisuke

American journal of hypertension

2010 Volume 23, Issue 8, Page(s) 821

MeSH term(s)	Animals ; Blood Pressure/drug effects ; Enzyme Induction ; Heme Oxygenase (Decyclizing)/biosynthesis ; Hypertension/drug therapy ; Medicine, Chinese Traditional ; Rats ; Triterpenes/therapeutic use
Chemical Substances	Triterpenes ; Heme Oxygenase (Decyclizing) (EC 1.14.14.18) ; Hmox1 protein, rat (EC 1.14.14.18) ; celastrol (L8GG98663L)
Language	English
Publishing date	2010-05-21
Publishing country	United States
Document type	Journal Article ; Comment
ZDB-ID	639383-4
ISSN	1941-7225 ; 1879-1905 ; 0895-7061
ISSN (online)	1941-7225 ; 1879-1905
ISSN	0895-7061
DOI	10.1038/ajh.2010.87
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Zs.A 2329: Show issues

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Article ; Online: Pseudomonas tohonis

Yamada, Kageto / Sasaki, Masakazu / Aoki, Kotaro / Nagasawa, Tatsuya / Murakami, Hinako / Ishii, Mayumi / Shibuya, Kazutoshi / Morita, Toshisuke / Ishii, Yoshikazu / Tateda, Kazuhiro

International journal of systematic and evolutionary microbiology

2021 Volume 71, Issue 11

Abstract: ... Strain ... ...

Abstract	Strain TUM18999
MeSH term(s)	Bacterial Typing Techniques ; Base Composition ; Burns/microbiology ; DNA, Bacterial/genetics ; Fatty Acids/chemistry ; Genes, Bacterial ; Humans ; Japan ; Phylogeny ; Pseudomonas/classification ; Pseudomonas/isolation & purification ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; Skin/microbiology
Chemical Substances	DNA, Bacterial ; Fatty Acids ; RNA, Ribosomal, 16S
Language	English
Publishing date	2021-11-11
Publishing country	England
Document type	Journal Article
ZDB-ID	2002336-4
ISSN	1466-5034 ; 1466-5026
ISSN (online)	1466-5034
ISSN	1466-5026
DOI	10.1099/ijsem.0.005115
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Zs.A 409: Show issues

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Article ; Online: Molecular and biochemical characterization of novel PAM-like MBL variants, PAM-2 and PAM-3, from clinical isolates of Pseudomonas tohonis.

Yamada, Kageto / Yoshizumi, Ayumi / Nagasawa, Tatsuya / Aoki, Kotaro / Sasaki, Masakazu / Murakami, Hinako / Morita, Toshisuke / Ishii, Yoshikazu / Tateda, Kazuhiro

The Journal of antimicrobial chemotherapy

2022 Volume 77, Issue 9, Page(s) 2414–2418

Abstract: Background: There is no comprehensive study on PAM-like MBLs.: Objectives: Our aim was to characterize novel B3 MBL variants, PAM-2 and PAM-3, from Pseudomonas tohonis clinical isolates.: Methods: We evaluated the antimicrobial susceptibility and ... ...

Abstract	Background: There is no comprehensive study on PAM-like MBLs. Objectives: Our aim was to characterize novel B3 MBL variants, PAM-2 and PAM-3, from Pseudomonas tohonis clinical isolates. Methods: We evaluated the antimicrobial susceptibility and the MBL gene composition of three novel P. tohonis clinical isolates identified at a Japanese hospital, using the broth microdilution method and WGS, respectively. We characterized the PAM-2 and PAM-3 proteins using recombinant protein expression and biochemical evaluations. Results: Low carbapenem MICs (meropenem MIC = 0.125-1 mg/L) were observed for all three P. tohonis isolates; however, the isolates produced MBLs. We identified blaPAM-2 and blaPAM-3 as potential genes, belonging to a novel subclass of B3 MBLs. Their genomic sequence was similar to that of blaPAM-1 from Pseudomonas alcaligenes. PAM-2 and PAM-3 comprised 287 amino acids and exhibited 90% amino acid identity with PAM-1, 73% identity with POM-1 from Pseudomonas otitidis and 61% identity with L1 from Stenotrophomonas maltophilia. Biochemical evaluations of recombinant PAM-2 and PAM-3 revealed similar kcat/Km ratios and demonstrated catalytic activity against all the tested β-lactams, except for aztreonam. In addition, the kcat/Km ratio for imipenem was 40-fold lower than that for meropenem. Conclusions: P. tohonis harbours a species-specific PAM-family MBL gene. This enzyme has higher hydrolytic activity against meropenem compared with that against imipenem.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Humans ; Imipenem/pharmacology ; Meropenem/pharmacology ; Microbial Sensitivity Tests ; Pseudomonas/genetics ; Pseudomonas Infections ; Pseudomonas aeruginosa/genetics ; beta-Lactamases/metabolism
Chemical Substances	Anti-Bacterial Agents ; Imipenem (71OTZ9ZE0A) ; beta-Lactamases (EC 3.5.2.6) ; Meropenem (FV9J3JU8B1)
Language	English
Publishing date	2022-06-30
Publishing country	England
Document type	Journal Article
ZDB-ID	191709-2
ISSN	1460-2091 ; 0305-7453
ISSN (online)	1460-2091
ISSN	0305-7453
DOI	10.1093/jac/dkac210
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Zs.A 1197: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 124: Show issues

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Article ; Online: Curcumin Inhibits Age-Related Vascular Changes in Aged Mice Fed a High-Fat Diet.

Takano, Kenichiro / Tatebe, Junko / Washizawa, Naohiro / Morita, Toshisuke

Nutrients

2018 Volume 10, Issue 10

Abstract: Inhibiting the onset of arteriosclerotic disease, which has been increasing due to the westernized diet and aging, is a significant social challenge. Curcumin, a type of polyphenol, has anti-oxidative effects and anti-inflammatory action and is expected ... ...

Abstract	Inhibiting the onset of arteriosclerotic disease, which has been increasing due to the westernized diet and aging, is a significant social challenge. Curcumin, a type of polyphenol, has anti-oxidative effects and anti-inflammatory action and is expected to treat and to have prophylactic effects on different diseases. In this study, we examined the effects of long-term administration of curcumin on vascular aging and chronic inflammation-the causes of arteriosclerotic disease. Eight-week-old C57BL/6J mice were fed with high fat diet (HFD) or 0.1% curcumin-mixed HFD (HFD + Cu) until 80 weeks old (
MeSH term(s)	Aging/drug effects ; Aging/metabolism ; Animals ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology ; Antioxidants/pharmacology ; Aorta/drug effects ; Aorta/metabolism ; Arteriosclerosis/prevention & control ; Curcumin/pharmacology ; Diet, High-Fat/adverse effects ; Inflammation ; Male ; Mice ; Mice, Inbred C57BL ; Oxidative Stress/drug effects ; Sirtuin 1/metabolism
Chemical Substances	Anti-Inflammatory Agents, Non-Steroidal ; Antioxidants ; Sirtuin 1 (EC 3.5.1.-) ; Curcumin (IT942ZTH98)
Language	English
Publishing date	2018-10-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu10101476
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Article ; Online: Evaluation of the simplified carbapenem inactivation method as a phenotypic detection method for carbapenemase-producing Enterobacterales.

Yamada, Kageto / Sasaki, Masakazu / Murakami, Hinako / Aoki, Kotaro / Morita, Toshisuke / Ishii, Yoshikazu / Tateda, Kazuhiro

Journal of microbiological methods

2021 Volume 187, Page(s) 106273

Abstract: Carbapenemase-producing Enterobacterales (CPE) have become a global health concern. Current molecular detection methods require special equipment and reagents. Thus, there is an urgent need for a highly sensitive, specific, and simple method for ... ...

Abstract	Carbapenemase-producing Enterobacterales (CPE) have become a global health concern. Current molecular detection methods require special equipment and reagents. Thus, there is an urgent need for a highly sensitive, specific, and simple method for phenotypic detection of CPE in clinical microbiology laboratories. A simplified carbapenem inactivation method (sCIM) was recently reported. However, its utility for CPE detection has not been sufficiently evaluated to date. We evaluated the sCIM and compared it with the modified CIM (mCIM), using 133 CPE strains (producing IMP, 92; NDM, 11; NDM and OXA-48-like, 1; KPC, 13; OXA-48-like, 12; GES-24, 3; Nmc-A, 1) and 82 non-CPE strains (extended spectrum β-lactamase, 61; AmpC, 21). The sCIM was conducted by loading bacteria onto imipenem and meropenem disks. When imipenem disks with a 1+ bacterial load were used, the sensitivity and specificity of the sCIM were 97.0% and 100%, and those of the mCIM were 97.0% and 96.3%, respectively. The specificity of the sCIM decreased to 57.3% when the bacterial load on imipenem disks was increased to 2+. In contrast, when meropenem disks with a 1+ bacterial load were used, the sCIM had a lower sensitivity (78.2%) and an equivalent specificity (100%). When meropenem disks with a bacterial load of 2+ were used, the sensitivity and specificity of the sCIM increased to 96.2% and 93.9%, respectively. The diameter of the inhibition zone on meropenem disks was larger than that on imipenem disks, and the sCIM was less sensitive when meropenem disks were used. In addition, sCIM detection rates when using meropenem disks were particularly low for OXA-48-like producers (bacterial load 1+, 0/12; bacterial load 2+, 10/12). Our results indicate that the sensitivity and specificity of the sCIM was dependent on the bacterial load and that large bacterial loads led to false positives for AmpC and extended spectrum β-lactamase producers. Thus, the sCIM has high sensitivity and specificity for appropriate bacterial loads when imipenem disks are used.
MeSH term(s)	Anti-Bacterial Agents/metabolism ; Anti-Bacterial Agents/pharmacology ; Bacterial Load ; Bacterial Proteins/metabolism ; Carbapenem-Resistant Enterobacteriaceae/drug effects ; Carbapenem-Resistant Enterobacteriaceae/enzymology ; Carbapenem-Resistant Enterobacteriaceae/isolation & purification ; False Positive Reactions ; Imipenem/metabolism ; Imipenem/pharmacology ; Meropenem/metabolism ; Meropenem/pharmacology ; Sensitivity and Specificity ; beta-Lactam Resistance ; beta-Lactamases/metabolism
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; Imipenem (71OTZ9ZE0A) ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6) ; Meropenem (FV9J3JU8B1)
Language	English
Publishing date	2021-06-23
Publishing country	Netherlands
Document type	Evaluation Study ; Journal Article
ZDB-ID	604916-3
ISSN	1872-8359 ; 0167-7012
ISSN (online)	1872-8359
ISSN	0167-7012
DOI	10.1016/j.mimet.2021.106273
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Zs.A 1849: Show issues

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Article: Evaluation of the simplified carbapenem inactivation method as a phenotypic detection method for carbapenemase-producing Enterobacterales

Yamada, Kageto / Sasaki, Masakazu / Murakami, Hinako / Aoki, Kotaro / Morita, Toshisuke / Ishii, Yoshikazu / Tateda, Kazuhiro

Journal of microbiological methods. 2021 Aug., v. 187

2021

Abstract	Carbapenemase-producing Enterobacterales (CPE) have become a global health concern. Current molecular detection methods require special equipment and reagents. Thus, there is an urgent need for a highly sensitive, specific, and simple method for phenotypic detection of CPE in clinical microbiology laboratories. A simplified carbapenem inactivation method (sCIM) was recently reported. However, its utility for CPE detection has not been sufficiently evaluated to date. We evaluated the sCIM and compared it with the modified CIM (mCIM), using 133 CPE strains (producing IMP, 92; NDM, 11; NDM and OXA-48–like, 1; KPC, 13; OXA-48–like, 12; GES-24, 3; Nmc-A, 1) and 82 non-CPE strains (extended spectrum β-lactamase, 61; AmpC, 21). The sCIM was conducted by loading bacteria onto imipenem and meropenem disks. When imipenem disks with a 1+ bacterial load were used, the sensitivity and specificity of the sCIM were 97.0% and 100%, and those of the mCIM were 97.0% and 96.3%, respectively. The specificity of the sCIM decreased to 57.3% when the bacterial load on imipenem disks was increased to 2+. In contrast, when meropenem disks with a 1+ bacterial load were used, the sCIM had a lower sensitivity (78.2%) and an equivalent specificity (100%). When meropenem disks with a bacterial load of 2+ were used, the sensitivity and specificity of the sCIM increased to 96.2% and 93.9%, respectively. The diameter of the inhibition zone on meropenem disks was larger than that on imipenem disks, and the sCIM was less sensitive when meropenem disks were used. In addition, sCIM detection rates when using meropenem disks were particularly low for OXA-48–like producers (bacterial load 1+, 0/12; bacterial load 2+, 10/12). Our results indicate that the sensitivity and specificity of the sCIM was dependent on the bacterial load and that large bacterial loads led to false positives for AmpC and extended spectrum β-lactamase producers. Thus, the sCIM has high sensitivity and specificity for appropriate bacterial loads when imipenem disks are used.
Keywords	equipment ; imipenem ; meropenem ; microbial load ; phenotype
Language	English
Dates of publication	2021-08
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	604916-3
ISSN	1872-8359 ; 0167-7012
ISSN (online)	1872-8359
ISSN	0167-7012
DOI	10.1016/j.mimet.2021.106273
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Article: ["How can hospitals develop a beneficial relationship with laboratory testing companies?" - Chairmen's introductory remarks].

Morita, Toshisuke / Kawano, Seiji

Rinsho byori. The Japanese journal of clinical pathology

2014 Volume 62, Issue 12, Page(s) 1245–1246

Abstract: ... chairing by Seiji Kawano, Kobe University and Toshisuke Morita, Toho University. Clinical testing is ...

Abstract	The symposium was held with the Japanese Society of Laboratory Medicine and JACLaP to discuss the way to develop a beneficial relationship between hospitals and laboratory testing companies with co-chairing by Seiji Kawano, Kobe University and Toshisuke Morita, Toho University. Clinical testing is considered to be essential for medical diagnosis and treatment; however, it is difficult for a hospital to perform all clinical testing for various reasons, including cost-effectiveness. In this session, 4 guest speakers gave a talk from their viewpoints. Doctor Kawano talked about the results of a questionnaire filled out by 114 university hospitals on how to develop a beneficial relationship between hospitalsoand laboratory testing companies. Next, Mr. Shinji Ogawa, president and CEO of SRL, talked about favorable ways to utilize laboratory testing companies, sayingthat such companies, which have a variety of skills, are expected to offer new and advanced technologies to hospitals continuously, and abundant data which laboratory testing companies have should be used for the advancement of community medicine. Professor Koshiba, Hyogo Medical School, expressed his apprehension to develop a so-called branch lab. in university hospitals from his own experience, and concluded that a beneficial relationship with companies to perform tasks required by hospitals should be sought. The last speaker, Yuichi Setoyama, Mitsubishi Chemical Medience, talked about the new relationship between hospitals and laboratory testing companies, and emphasized that hospitals and such companies should know the strong and weak points of each other and build a mutually complementary system. After all presentations were over, a discussion with participants was held. Doctors of clinics said that the role of laboratory testing companies for large hospitals is different from that for small clinics, and such companies are indispensable for his everyday medical activities. Each medical institute has its own medical mission, and, therefore, what constitutes a beneficial relationship varies with each medical institute. The key to the success of building a win-win relationship with laboratory testing companies is held by each hospital. (Review).
MeSH term(s)	Hospitals ; Medical Laboratory Science ; Schools, Medical
Language	Japanese
Publishing date	2014-12
Publishing country	Japan
Document type	English Abstract ; Journal Article ; Review
ZDB-ID	604196-6
ISSN	0047-1860 ; 0485-1404
ISSN	0047-1860 ; 0485-1404
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