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  1. Article ; Online: Health Benefits from Upgrading Public Buses for Cleaner Air: A Case Study of Clark County, Nevada and the United States.

    Olawepo, John O / Chen, L-W Antony

    International journal of environmental research and public health

    2019  Volume 16, Issue 5

    Abstract: ... operating in Clark County, Nevada were CNG-powered. This study assesses the health benefits of the venture ...

    Abstract Public transit buses, which move more than 5 billion passengers annually in the United States (U.S.), can contribute substantially to the environmental health burden through emitted air pollutants. As a leader in transforming to cleaner bus fleets, the Regional Transport Commission of Southern Nevada (RTC) has been transitioning from diesel to compressed natural gas (CNG) transit buses since 1999. By 2017, ~75% of RTC's buses operating in Clark County, Nevada were CNG-powered. This study assesses the health benefits of the venture using the US Environmental Protection Agency's (EPA) Co-Benefits Risk Assessment (COBRA) model, considering the emission and exposure changes from the 2017 baseline for two hypothetical scenarios: (1) no transition (CC_D) and (2) complete transition (CC_N). The CC_D scenario shows realized health benefits, mostly due to avoided mortality, of $0.79⁻8.21 million per year for 2017 alone, while CC_N suggests an additional $0.88⁻2.24 million annually that could be achieved by completing the transition. The wide range of estimates partly reflects uncertainties in determining diesel bus emissions under business-as-usual. These health benefits were not limited locally, with ~70% going to other counties. Two national-scale scenarios, US_D and US_N, were also constructed to explore the health impact of transitioning from diesel to CNG buses across the U.S. As of 2017, with CNG powering only ~20% of transit bus mileages nationwide, there could be massive unrealized health benefits of $0.98⁻2.48 billion per year including 114⁻258 avoided premature deaths and >5000 avoided respiratory and cardiovascular illnesses. Taking into account the health benefits, economic costs, and the inter-state nature of air pollution, expanding federal assistances to accelerate a nationwide transition to cleaner bus fleets is highly recommended.
    MeSH term(s) Air Pollutants/analysis ; Humans ; Inhalation Exposure/analysis ; Inhalation Exposure/economics ; Inhalation Exposure/prevention & control ; Motor Vehicles ; Natural Gas ; Nevada ; Risk Assessment ; United States ; Vehicle Emissions/analysis
    Chemical Substances Air Pollutants ; Natural Gas ; Vehicle Emissions
    Language English
    Publishing date 2019-02-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1660-4601
    ISSN (online) 1660-4601
    DOI 10.3390/ijerph16050720
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Direct determination of p-nitrophenyl substituent organophosphorus nerve agents using a recombinant Pseudomonas putida JS444-modified Clark oxygen electrode.

    Lei, Yu / Mulchandani, Priti / Chen, Wilfred / Mulchandani, Ashok

    Journal of agricultural and food chemistry

    2005  Volume 53, Issue 3, Page(s) 524–527

    Abstract: A microbial biosensor for rapid, sensitive, selective, and cost-effective determination of the total content of organophosphorus nerve agents with p-nitrophenyl substituent is reported. The biosensor consisted of genetically engineered PNP-degrader ... ...

    Abstract A microbial biosensor for rapid, sensitive, selective, and cost-effective determination of the total content of organophosphorus nerve agents with p-nitrophenyl substituent is reported. The biosensor consisted of genetically engineered PNP-degrader Pseudomonas putida JS444 expressing organophosphorus hydrolase (OPH) on its cell surface immobilized on a dissolved oxygen electrode. Surface-expressed OPH catalyzed the hydrolysis of organophosphorus pesticides with p-nitrophenyl substituent such as paraoxon, methyl parathion, and parathion to release p-nitrophenol that was oxidized by the enzymatic machinery of Pseudomonas putida JS444 to carbon dioxide while consuming oxygen. The oxygen consumption was measured and correlated to the concentration of organophosphates. The sensor signal and response time were optimized with 0.086 mg dry weight of cell and operating in 50 mM pH 7.5 citrate-phosphate buffer with 50 microM CoCl(2) at room temperature. When operated at optimized conditions, the biosensor measured as low as 55 ppb of paraoxon, 53 ppb of methyl parathion, and 58 ppb of parathion without interference from most phenolic compounds and other commonly used pesticides, such as atrazine, coumaphos, sutan, sevin, and diazinon. The operational life of the microbial biosensor was approximately 5 days when stored in the operating buffer at 4 degrees C.
    MeSH term(s) Aryldialkylphosphatase/genetics ; Biosensing Techniques ; Insecticides/analysis ; Methyl Parathion/analysis ; Organophosphorus Compounds/analysis ; Oxygen ; Paraoxon/analysis ; Parathion/analysis ; Pseudomonas putida/enzymology ; Pseudomonas putida/genetics ; Recombinant Proteins ; Sensitivity and Specificity
    Chemical Substances Insecticides ; Organophosphorus Compounds ; Recombinant Proteins ; Methyl Parathion (41BCL2O91D) ; Parathion (61G466064D) ; Aryldialkylphosphatase (EC 3.1.8.1) ; Paraoxon (Q9CX8P80JW) ; Oxygen (S88TT14065)
    Language English
    Publishing date 2005-02-09
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/jf048943t
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Leveraging Endogenous Pain Modulation for Analgesia.

    Chen, QiLiang / Clark, J David

    Anesthesiology

    2024  Volume 140, Issue 2, Page(s) 192–194

    MeSH term(s) Humans ; Pain ; Analgesia ; Pain Management
    Language English
    Publishing date 2024-01-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 269-0
    ISSN 1528-1175 ; 0003-3022
    ISSN (online) 1528-1175
    ISSN 0003-3022
    DOI 10.1097/ALN.0000000000004812
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book ; Online: New Research Directions in DNA Repair

    Chen, Clark

    2013  

    Keywords Medical genetics
    Size 1 electronic resource (674 pages)
    Publisher IntechOpen
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021046097
    ISBN 9789535153757 ; 9535153757
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  5. Book ; Online: Advances in DNA Repair

    Chen, Clark C

    2015  

    Keywords Medical genetics
    Language English
    Size 1 electronic resource (496 pages)
    Publisher IntechOpen
    Document type Book ; Online
    Note English
    HBZ-ID HT030645808
    ISBN 9789535142133 ; 9535142135
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  6. Article ; Online: Biosensor for Direct Determination of Fenitrothion and EPN Using Recombinant Pseudomonas putida JS444 with Surface Expressed Organophosphorus Hydrolase. 1. Modified Clark Oxygen Electrode

    Ashok Mulchandani / Wilfred Chen / Priti Mulchandani / Yu Lei

    Sensors, Vol 6, Iss 4, Pp 466-

    2006  Volume 472

    Abstract: This paper reports a first microbial biosensor for rapid and cost-effectivedetermination of organophosphorus pesticides fenitrothion and EPN. The biosensorconsisted of recombinant PNP-degrading/oxidizing bacteria Pseudomonas putida JS444anchoring and ... ...

    Abstract This paper reports a first microbial biosensor for rapid and cost-effectivedetermination of organophosphorus pesticides fenitrothion and EPN. The biosensorconsisted of recombinant PNP-degrading/oxidizing bacteria Pseudomonas putida JS444anchoring and displaying organophosphorus hydrolase (OPH) on its cell surface asbiological sensing element and a dissolved oxygen electrode as the transducer. Surface-expressed OPH catalyzed the hydrolysis of fenitrothion and EPN to release 3-methyl-4-nitrophenol and p-nitrophenol, respectively, which were oxidized by the enzymaticmachinery of Pseudomonas putida JS444 to carbon dioxide while consuming oxygen,which was measured and correlated to the concentration of organophosphates. Under theoptimum operating conditions, the biosensor was able to measure as low as 277 ppb offenitrothion and 1.6 ppm of EPN without interference from phenolic compounds and othercommonly used pesticides such as carbamate pesticides, triazine herbicides andorganophosphate pesticides without nitrophenyl substituent. The applicability of thebiosensor to lake water was also demonstrated.
    Keywords Organophosphorus ; fenitrothion ; EPN ; biosensor ; Pseudomonas putida. ; Technology (General) ; T1-995 ; Technology ; T ; DOAJ:Technology (General) ; DOAJ:Technology and Engineering ; Analytical chemistry ; QD71-142 ; Chemistry ; QD1-999 ; Science ; Q ; DOAJ:Analytical Chemistry ; DOAJ:Chemistry
    Subject code 540
    Language English
    Publishing date 2006-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Direct determination of p-nitrophenyl substituent organophosphorus nerve agents using a recombinant Pseudomonas putida JS444-modified Clark oxygen electrode

    Lei, Y / Mulchandani, P / Chen, W / Mulchandani, A

    Journal of agricultural and food chemistry. 2005 Feb. 9, v. 53, no. 3

    2005  

    Abstract: A microbial biosensor for rapid, sensitive, selective, and cost-effective determination of the total content of organophosphorus nerve agents with p-nitrophenyl substituent is reported. The biosensor consisted of genetically engineered PNP-degrader ... ...

    Abstract A microbial biosensor for rapid, sensitive, selective, and cost-effective determination of the total content of organophosphorus nerve agents with p-nitrophenyl substituent is reported. The biosensor consisted of genetically engineered PNP-degrader Pseudomonas putida JS444 expressing organophosphorus hydrolase (OPH) on its cell surface immobilized on a dissolved oxygen electrode. Surface-expressed OPH catalyzed the hydrolysis of organophosphorus pesticides with p-nitrophenyl substituent such as paraoxon, methyl parathion, and parathion to release p-nitrophenol that was oxidized by the enzymatic machinery of Pseudomonas putida JS444 to carbon dioxide while consuming oxygen. The oxygen consumption was measured and correlated to the concentration of organophosphates. The sensor signal and response time were optimized with 0.086 mg dry weight of cell and operating in 50 mM pH 7.5 citrate-phosphate buffer with 50 micromolar CoCl2 at room temperature. When operated at optimized conditions, the biosensor measured as low as 55 ppb of paraoxon, 53 ppb of methyl parathion, and 58 ppb of parathion without interference from most phenolic compounds and other commonly used pesticides, such as atrazine, coumaphos, sutan, sevin, and diazinon. The operational life of the microbial biosensor was approximately 5 days when stored in the operating buffer at 4 degrees C.
    Keywords organophosphorus compounds ; toxic substances ; parathion ; parathion-methyl ; paraoxon ; biosensors ; Pseudomonas putida ; immobilized enzymes ; genetically engineered microorganisms ; oxygen ; electrodes
    Language English
    Dates of publication 2005-0209
    Size p. 524-527.
    Document type Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    Database NAL-Catalogue (AGRICOLA)

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  8. Book ; Online: Advances in the Biology, Imaging and Therapies for Glioblastoma

    Chen, Clark C.

    2011  

    Keywords Oncology
    Size 1 electronic resource (436 pages)
    Publisher IntechOpen
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021045963
    ISBN 9789535165590 ; 9535165593
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  9. Book ; Online: Selected Topics in DNA Repair

    Chen, Clark C.

    2011  

    Keywords Medical genetics
    Size 1 electronic resource (584 pages)
    Publisher IntechOpen
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021046114
    ISBN 9789535165361 ; 9535165364
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  10. Article: Editorial: Molecular advances in diagnosis and treatment of CNS tumors, volume II.

    Chen, Clark / Chen, Liam

    Frontiers in oncology

    2023  Volume 13, Page(s) 1146805

    Language English
    Publishing date 2023-01-30
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2023.1146805
    Database MEDical Literature Analysis and Retrieval System OnLINE

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