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Article ; Online: eDNA Adsorption onto Microplastics: Impacts of Water Chemistry and Polymer Physiochemical Properties.

Müller, Nicolas D / Kirtane, Anish / Schefer, Roman B / Mitrano, Denise M

Environmental science & technology

2024 Volume 58, Issue 17, Page(s) 7588–7599

Abstract: ... before eventually reaching equilibrium after 1-2 h. Overall, we demonstrated that DNA quickly binds to MPs ...

Abstract	Adsorption of biomacromolecules onto polymer surfaces, including microplastics (MPs), occurs in multiple environmental compartments, forming an ecocorona. Environmental DNA (eDNA), genetic material shed from organisms, can adsorb onto MPs which can potentially either (1) promote long-range transport of antibiotic resistant genes or (2) serve to gain insights into the transport pathways and origins of MPs by analyzing DNA sequences on MPs. However, little is known about the capacity of MPs to adsorb eDNA or the factors that influence sorption, such as polymer and water chemistries. Here we investigated the adsorption of extracellular linear DNA onto a variety of model MP fragments composed of three of the most environmentally prevalent polymers (polyethylene, polyethylene terephthalate, and polystyrene) in their pristine and photochemically weathered states. Batch adsorption experiments in a variety of water chemistries were complemented with nonlinear modeling to quantify the rate and extent of eDNA sorption. Ionic strength was shown to strongly impact DNA adsorption by reducing or inhibiting electrostatic repulsion. Polyethylene terephthalate exhibited the highest adsorption capacity when normalizing for MP specific surface area, likely due to the presence of ester groups. Kinetics experiments showed fast adsorption (majority adsorbed under 30 min) before eventually reaching equilibrium after 1-2 h. Overall, we demonstrated that DNA quickly binds to MPs, with pseudo-first- and -second-order models describing adsorption kinetics and the Freundlich model describing adsorption isotherms most accurately. These insights into DNA sorption onto MPs show that there is potential for MPs to act as vectors for genetic material of interest, especially considering that particle-bound DNA typically persists longer in the environment than dissolved DNA.
MeSH term(s)	Adsorption ; Microplastics/chemistry ; DNA, Environmental ; Polymers/chemistry ; Water/chemistry ; DNA/chemistry
Chemical Substances	Microplastics ; DNA, Environmental ; Polymers ; Water (059QF0KO0R) ; DNA (9007-49-2)
Language	English
Publishing date	2024-04-16
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.3c10825
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Biochemically assisted rice whitening for improving head rice yield

Schefer, Larissa / Conde‐Petit, Béatrice / Müller, Nadina

Journal of food process engineering. 2021 Nov., v. 44, no. 11

2021

Abstract: ... in an estimated increase in profit for a 7.5 t/h rice mill of 0.83%. PRACTICAL APPLICATIONS: Rice as a global ...

Abstract	The maximum attainable head rice yield in conventional long grain rice milling is approximately 64%, with around 15% being lost as a result of broken rice kernels. The primary objective of this project was, therefore, to improve the milling yield. To achieve this goal, biochemically assisted whitening processes involving the application of different aqueous solutions were evaluated. Head rice yield was increased for all tested liquids (3.3–3.8% depending on liquid) for Gladio‐type brown rice treated with 0.5% liquid prior to whitening to 40 Kett using a lab‐scale horizontal friction‐type McGill whitener. However, the moistening led to increased caking in the McGill milling chamber. In comparative trials, the use of moistening solutions containing enzymes, sorbit, or sodium chloride instead of pure water delivered a slightly, but nevertheless, significantly higher degree of whiteness directly after milling while it did not result in a significant reduction in the number of broken kernels. Since average head rice yield has a 43% higher commercial value than broken kernels, the 3.6% improvement in milling yield achieved by adding 0.5% water would result in an estimated increase in profit for a 7.5 t/h rice mill of 0.83%. PRACTICAL APPLICATIONS: Rice as a global staple food bears a critical role in human nutrition. At the same time, the quality of milled rice is a key buying and price criterion in rice‐consuming countries. One key quality criterion is the number of brokens in rice. Hence, it is critical for rice millers to minimize the degree of broken kernels. Biochemically assisted rice whitening for improving head rice yield is a combined biochemical and physical method to facilitate bran removal from brown rice. The main aim of the present study was to investigate the effect of biochemically assisted rice whitening on the number of brokens and to assess potential technological challenges resulting from the liquid addition.
Keywords	bleaching agents ; brown rice ; debranning ; human nutrition ; liquids ; prices ; sodium chloride ; staple foods
Language	English
Dates of publication	2021-11
Publishing place	John Wiley & Sons, Inc.
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	752358-0
ISSN	0145-8876
ISSN	0145-8876
DOI	10.1111/jfpe.13850
Database	NAL-Catalogue (AGRICOLA)

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Article: Small-angle neutron scattering from the reconstituted TF1 of H(+)-ATPase from thermophilic bacterium PS3 with deuterated subunits.

Ito, Y / Harada, M / Ohta, S / Kagawa, Y / Aono, O / Schefer, J / Schoenborn, B P

Journal of molecular biology

1990 Volume 213, Issue 2, Page(s) 289–302

Abstract: Subunits alpha, beta and gamma of adenosine triphosphatase (H ...

Abstract	Subunits alpha, beta and gamma of adenosine triphosphatase (H(+)-ATPase) from the thermophilic bacterium PS3 (TF1) have been over-expressed in Escherichia coli. alpha and beta subunits deuterated to the level of 90% were obtained by culturing E. coli in 2H2O medium. Both the subunits and the reconstituted alpha beta gamma complex, TF1, which contain the deuterated components in various combinations, were studied in solution by small-angle neutron scattering. The individual shapes of the subunits and their organization in the alpha beta gamma-TF1 complex were examined using the techniques of selective deuteration and contrast variation. The alpha and beta subunits are well approximated as ellipsoids of revolution having minor semi-axes of 20.4(+/- 0.4) and 20.0(+/- 0.2) A, and major semi-axes of 53.0(+/- 1.4) and 55.8(+/- 0.9) A, respectively. In the TF1 complex, three beta subunits are aligned to form an equilateral triangle, with their major axes tilted by 35 degrees with respect to the 3-fold axis of the complex. The beta-beta distance is about 53 A. Three alpha subunits are similarly arranged, positioned between the beta subunits, and with their direction of tilt opposite to that of the beta subunits. The centers of the alpha and beta subunits lie in the same plane, forming a hexagon. Adjacent subunits overlap in this model, suggesting that they are not simple ellipsoids of revolution.
MeSH term(s)	Bacteria/enzymology ; Chemical Phenomena ; Chemistry, Physical ; Deuterium ; Escherichia coli/genetics ; Gene Expression Regulation, Bacterial ; Neutrons ; Proton-Translocating ATPases/metabolism ; Scattering, Radiation
Chemical Substances	Deuterium (AR09D82C7G) ; Proton-Translocating ATPases (EC 3.6.3.14)
Language	English
Publishing date	1990-05-20
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	80229-3
ISSN	1089-8638 ; 0022-2836
ISSN (online)	1089-8638
ISSN	0022-2836
DOI	10.1016/S0022-2836(05)80191-3
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Article ; Online: High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications.

Alter, Claudio L / Detampel, Pascal / Schefer, Roman B / Lotter, Claudia / Hauswirth, Patrick / Puligilla, Ramya D / Weibel, Vera J / Schenk, Susanne H / Heusermann, Wolf / Schürz, Melanie / Meisner-Kober, Nicole / Palivan, Cornelia / Einfalt, Tomaž / Huwyler, Jörg

Communications biology

2023 Volume 6, Issue 1, Page(s) 478

Abstract: Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a highly efficient and reproducible ... ...

Abstract	Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a highly efficient and reproducible EV preparation method for monodisperse nano plasma membrane vesicles (nPMVs), which yields 10 to 100 times more particles per cell and hour than conventional EV preparation methods. nPMVs are produced by homogenizing giant plasma membrane vesicles following cell membrane blebbing and apoptotic body secretion induced by chemical stressors. nPMVs showed no significant differences compared to native EVs from the same cell line in cryo-TEM analysis, in vitro cellular interactions, and in vivo biodistribution studies in zebrafish larvae. Proteomics and lipidomics, on the other hand, suggested substantial differences consistent with the divergent origin of these two EV types and indicated that nPMVs primarily derive from apoptotic extracellular vesicles. nPMVs may provide an attractive source for developing EV-based pharmaceutical therapeutics.
MeSH term(s)	Animals ; Reproducibility of Results ; Tissue Distribution ; Zebrafish ; Extracellular Vesicles/metabolism ; Cell Membrane/metabolism
Language	English
Publishing date	2023-05-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-023-04859-2
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Article ; Online: A institucionalização das práticas de responsabilidade social corporativa em concessionárias automotivas

Taísa Schefer Roveda / Maria Margarete Baccin Brizolla

Research, Society and Development, Vol 9, Iss 7, Pp e768974367-e

2020 Volume 768974367

Abstract: O comportamento das organizações está associado a um conjunto de regras e normas presentes nos processos de troca entre elas e o ambiente. A institucionalização das práticas de responsabilidade social corporativa (RSC) tem avançado atualmente. Com isso, ... ...

Abstract	O comportamento das organizações está associado a um conjunto de regras e normas presentes nos processos de troca entre elas e o ambiente. A institucionalização das práticas de responsabilidade social corporativa (RSC) tem avançado atualmente. Com isso, este estudo objetiva verificar quais os mecanismos isomórficos presentes na institucionalização das práticas de RSC. Para tanto, utilizou-se da pesquisa descritiva, estudo de campo e abordagem quantitativa. Como instrumento de coleta dos dados foi feito uso do questionário com escala Likert de cinco pontos enviado por e-mail. A população estudada são as concessionárias automotivas do Rio Grande do Sul, sendo que a amostra se constitui em 24 empresas, que responderam o instrumento. Os achados indicam que para as dimensões econômica e legal a institucionalização das práticas de RSC de acordo com a percepção dos gestores é de intensidade forte a fortíssima e para a dimensão ética a intensidade das práticas de RSC é de média a forte. O percebido pelos colaboradores indica intensidade forte a fortíssima nas práticas de RSC para a dimensão econômica e intensidade média a forte para as dimensões legal e ética. Para os gestores o isomorfismo que mais interfere nas práticas de RSC é o coercitivo com intensidade média a forte e o que menos interfere é o mimético com intensidade de presença forte. A percepção dos colaboradores indica que os mecanismos isomórficos coercitivos, mimético e normativo tem participação semelhante na institucionalização das práticas de RSC, com intensidade média a forte.
Keywords	responsabilidade social corporativa ; isomorfismo ; concessionárias automotivas ; Special aspects of education ; LC8-6691 ; Science (General) ; Q1-390 ; Social Sciences ; H
Language	English
Publishing date	2020-06-01T00:00:00Z
Publisher	Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effects of different stimulators of cGMP synthesis on lipid content in bovine oocytes matured

Schefer, Letícia / Schwarz, Kátia Regina Lancelloti / Paschoal, Daniela Martins / de Castro, Fernanda Cavallari / Fernandes, Hugo / Botigelli, Ramon César / Leal, Cláudia Lima Verde

Animal reproduction

2021 Volume 18, Issue 4, Page(s) e20210072

Abstract: Bovine oocytes and blastocysts ... ...

Abstract	Bovine oocytes and blastocysts produced
Language	English
Publishing date	2021-12-10
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2227229-X
ISSN	1984-3143 ; 1984-3143
ISSN (online)	1984-3143
ISSN	1984-3143
DOI	10.1590/1984-3143-AR2021-0072
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Article ; Online: Microangiopathic haemolytic anaemia in a patient with metastatic breast cancer.

Himmelmann, Andreas / Schefer, Hubert

British journal of haematology

2009 Volume 146, Issue 3, Page(s) 231

MeSH term(s)	Aged ; Anemia, Hemolytic/complications ; Anemia, Hemolytic/pathology ; Breast Neoplasms/complications ; Fatal Outcome ; Female ; Humans ; Neoplastic Cells, Circulating
Language	English
Publishing date	2009-08
Publishing country	England
Document type	Case Reports ; Journal Article
ZDB-ID	80077-6
ISSN	1365-2141 ; 0007-1048
ISSN (online)	1365-2141
ISSN	0007-1048
DOI	10.1111/j.1365-2141.2008.07575.x
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Article ; Online: Diffusion-weighted MRI of ischemic stroke at 3T: Value of synthetic

Sartoretti, Thomas / Sartoretti, Elisabeth / Wyss, Michael / Mannil, Manoj / van Smoorenburg, Luuk / Eichenberger, Barbara / Reischauer, Carolin / Alfieri, Alex / Binkert, Christoph / Sartoretti-Schefer, Sabine

The British journal of radiology

2021 Volume 94, Issue 1121, Page(s) 20200869

Abstract: ... on DWI performed within 24 h of symptom onset. B-values of 0, 500, 1000 and 2000 s mm: Results ...

Abstract	Objectives: Diffusion-weighted imaging (DWI) plays a crucial role in the diagnosis of ischemic stroke. We assessed the value of computed and acquired high b-value DWI in comparison with conventional Methods: We included 36 patients with acute ischemic stroke who presented with diffusion abnormalities on DWI performed within 24 h of symptom onset. B-values of 0, 500, 1000 and 2000 s mm Results: Syn1000 and syn1500 matched acq1000 images in terms of lesion detection rate, image quality and presence of uncertain hyperintensities but presented with significantly improved lesion conspicuity ( Conclusion: Synthetic images at Advances in knowledge: Synthetic b-values enable improved lesion conspicuity for DWI of ischemic stroke.
MeSH term(s)	Aged ; Aged, 80 and over ; Diffusion Magnetic Resonance Imaging/methods ; Diffusion Magnetic Resonance Imaging/standards ; Female ; Humans ; Ischemic Stroke/diagnostic imaging ; Male ; Middle Aged ; Reference Standards ; Retrospective Studies ; Signal-To-Noise Ratio ; Uncertainty
Language	English
Publishing date	2021-02-17
Publishing country	England
Document type	Comparative Study ; Journal Article
ZDB-ID	2982-8
ISSN	1748-880X ; 0007-1285
ISSN (online)	1748-880X
ISSN	0007-1285
DOI	10.1259/bjr.20200869
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Article ; Online: Repetitive daily point of choice prompts and occupational sit-stand transfers, concentration and neuromuscular performance in office workers: an RCT.

Donath, Lars / Faude, Oliver / Schefer, Yannick / Roth, Ralf / Zahner, Lukas

International journal of environmental research and public health

2015 Volume 12, Issue 4, Page(s) 4340–4353

Abstract: ... h (4.8) to 9.7 (6.6) h (p = 0.07). Concentration and neuromuscular performance did not change ...

Abstract	Objective: Prolonged office sitting time adversely affects neuromuscular and cardiovascular health parameters. As a consequence, the present study investigated the effects of prompting the use of height-adjustable working desk (HAWD) on occupational sitting and standing time, neuromuscular outcomes and concentration in office workers. Methods: A single-blinded randomized controlled trial (RCT) with parallel group design was conducted. Thirty-eight office workers were supplied with HAWDs and randomly assigned (Strata: physical activity (PA), BMI, gender, workload) to a prompt (INT) or non-prompt (CON) group. INT received three daily screen-based prompts within 12 weeks. CON was only instructed once concerning the benefits of using HAWDs prior to the start of the study. Sitting and standing times were objectively assessed as primary outcomes for one entire working week using the ActiGraph wGT3X-BT at baseline (pre), after 6 (mid) and 12 weeks (post). Concentration (d2-test), postural sway during upright stance (under single, dual and triple task) and lower limb strength endurance (heel-rise) were collected as secondary outcomes. Results: With large but not statistically significant within group effects from pre to post, INT increased weekly standing time at work by 9% (p = 0.22, d = 0.8) representing an increase from 7.2 h (4.8) to 9.7 (6.6) h (p = 0.07). Concentration and neuromuscular performance did not change from pre to post testing (0.23 < p < 0.95; 0.001 < ηp² < 0.05). Conclusion: Low-frequent and low cost screen-based point of choice prompts (3 per day within 12 weeks) already result in notable increases of occupational standing time of approx. daily 30 min. These stimuli, however, did not relevantly affect neuromuscular outcomes.
MeSH term(s)	Adult ; Attention ; Choice Behavior ; Female ; Humans ; Lower Extremity/physiology ; Male ; Middle Aged ; Physical Endurance ; Posture ; Sedentary Lifestyle ; Work ; Workplace
Language	English
Publishing date	2015-04-20
Publishing country	Switzerland
Document type	Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
ISSN	1660-4601
ISSN (online)	1660-4601
DOI	10.3390/ijerph120404340
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Das ärztliche Ethos bei Paracelsus.

Schefer, H

Nova acta Paracelsica : ... Jahrbuch der Schweizerischen Paracelsus-Gesellschaft

1993 Volume 7, Page(s) 71–92

MeSH term(s)	Ethics, Medical/history ; History, Early Modern 1451-1600 ; History, Modern 1601- ; Medicine ; Switzerland
Language	German
Publishing date	1993
Publishing country	Switzerland
Document type	Biography ; Historical Article ; Journal Article
ZDB-ID	210354-0
ISSN	0254-8712
ISSN	0254-8712
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