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Article ; Online: Meet the authors: Meiling Wang, Wenjing Li, and Weixing Zhao.

Wang, Meiling / Li, Wenjing / Zhao, Weixing

2023 Volume 83, Issue 20, Page(s) 3578–3581

Abstract: Molecular Cell talks to co-first authors Meiling Wang and Wenjing Li with co-corresponding author ...

Abstract	Molecular Cell talks to co-first authors Meiling Wang and Wenjing Li with co-corresponding author Weixing Zhao about their paper, "Crucial roles of the BRCA1-BARD1 E3 ubiquitin ligase activity in homology-directed DNA repair" (in this issue of Molecular Cell) and what motivates their scientific pursuits.
MeSH term(s)	Tumor Suppressor Proteins/genetics ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism ; Ubiquitination
Chemical Substances	Tumor Suppressor Proteins ; Ubiquitin-Protein Ligases (EC 2.3.2.27)
Language	English
Publishing date	2023-11-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	1415236-8
ISSN	1097-4164 ; 1097-2765
ISSN (online)	1097-4164
ISSN	1097-2765
DOI	10.1016/j.molcel.2023.09.036
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Article: [Effect of Liangfang Wenjing Decoction on expression of key glycolytic enzymes in uterus and ovaries of rats with coagulating cold and blood stasis syndrome].

Guo, Jin-Yu / Xing, Yi / Liu, Peng / Wang, Di / Cheng, Xiu-Mei / Wang, Xin-Guo / Ren, Yan-Qing

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2023 Volume 48, Issue 12, Page(s) 3215–3223

Abstract: ... and glycolysis, and observe the intervention effect of Liangfang Wenjing Decoction(LFWJD ...

Abstract	This study aimed to investigate the relationship between coagulating cold and blood stasis syndrome and glycolysis, and observe the intervention effect of Liangfang Wenjing Decoction(LFWJD) on the expression of key glycolytic enzymes in the uterus and ovaries of rats with coagulating cold and blood stasis. The rat model of coagulating cold and blood stasis syndrome was established by ice-water bath. After modeling, the quantitative scoring of symptoms were performed, and according to the scoring results, the rats were randomly divided into a model group and LFWJD low-, medium-and high-dose groups(4.7, 9.4, 18.8 g·kg~(-1)·d~(-1)), with 10 in each group. Another 10 rats were selected as the blank group. After 4 weeks of continuous administration by gavage, the quantitative scoring of symptoms was repeated. Laser speckle flowgraphy was used to detect the changes of microcirculation in the ears and uterus of rats in each group. Hematoxylin-eosin(HE) staining was used to observe the pathological morphology of uterus and ovaries of rats in each group. The mRNA and protein expressions of pyruvate dehydrogenase kinase 1(PDK1), hexokinase 2(HK2) and lactate dehydrogenase A(LDHA) in the uterus and ovaries of rats were examined by real-time quantitative polymerase chain reaction(RT-qPCR) and Western blot, respectively. The rats in the model group showed signs of coagulating cold and blood stasis syndrome, such as curl-up, less movement, thickened veins under the tongue, and reduced blood perfusion in the microcirculation of the ears and uterus, and HE staining revealed a thinning of the endometrium with disorganized arrangement of epithelial cells and a decrease in the number of ovarian follicles. Compared with the model group, the treatment groups had alleviated coagulating cold and blood stasis, which was manifested as red tongue, reduced nail swelling, no blood stasis at the tail end as well as increased blood perfusion of the microcirculation in the ears and uterus(P<0.05 or P<0.01). Among the groups, the LFWJD medium-and high-dose groups had the most significant improvement in coagulating cold and blood stasis, with neatly arranged columnar epithelial cells in uterus, and the number of ovarian follicles was higher than that in the model group, especially mature follicles. The mRNA and protein expressions of PDK1, HK2, LDHA in uterus and ovaries were up-regulated in the model group(P<0.05 or P<0.01), while down-regulated in LFWJD medium-and high-dose groups(P<0.05 or P<0.01). The LFWJD low-dose group presented a decrease in the mRNA expressions of PDK1, HK2 and LDHA in uterus and ovaries as well as in the protein expressions of HK2 and LDHA in uterus and HK2 and PDK1 in ovaries(P<0.05 or P<0.01). The therapeutic mechanism of LFWJD against coagulating cold and blood stasis syndrome is related to the down-regulation of key glycolytic enzymes PDK1, HK2 and LDHA, and the inhibition of glycolytic activities in uterus and ovaries.
MeSH term(s)	Female ; Animals ; Rats ; Ovary ; Uterus ; Ovarian Follicle ; Lactate Dehydrogenase 5 ; Glycolysis
Chemical Substances	Wenjing decoction ; Lactate Dehydrogenase 5 (EC 1.1.1.27.-)
Language	Chinese
Publishing date	2023-06-29
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.19540/j.cnki.cjcmm.20230223.401
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Article: [Spray drying process of extract of Wenjing Zhitong Prescription based on mixture design experiment].

Zhu, Yu-Chao / Cheng, Jian-Ming / Yan, Yuan-Yuan / He, Rui-Xin / Wang, Qi / Xu, Ya-Die / Zhang, Xiao-Yun

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2020 Volume 45, Issue 1, Page(s) 98–105

Abstract: To improve the spray drying effect of extract of Wenjing Zhitong Prescription, this study takes ... 05; according to the optimized drying process parameters of Wenjing Zhitong Prescription, initial ...

Abstract	To improve the spray drying effect of extract of Wenjing Zhitong Prescription, this study takes the yield, hygroscopic property and the fluidity of dry powder as indexes to screen out auxiliary materials, and the proportion of the auxiliary materials was optimized based on the mixing design experiment; based on that, HPLC method was established for the determination of glycyrrhizin and 6-gingerol in spray powder, the yield of spray powder and the retention rate of the two index components were taken as indexes to further optimize the spray drying parameters. The finally selected auxiliary materials were light magnesium oxide, maltodextrin and silica, and regression equations of dry powder yield, moisture absorption rate, angle of rest with proportion of auxiliary materials were established, and the optimized proportion of auxiliary materials was dry paste-light magnesium oxide-maltodextrin-silica=0.5∶0.305∶0.145∶0.05; according to the optimized drying process parameters of Wenjing Zhitong Prescription, initial temperature was 60 ℃, air inlet temperature was 130 ℃, air flow rate was 35 m~3·h~(-1), atomizing pressure was 40 mm, and liquid inlet speed was 4.5 mL·min~(-1). Under these conditions, the dry powder yield was 90.28%, the retention rate of glycyrrhizin was 74.51%, and the retention rate of 6-gingerol was 72.10%. In this study, optimized auxiliary materials can improve the yield of spray drying and the property of spray powder, and the optimized processing conditions were good for retaining the unstable gingerol components, which can lay a foundation for the further preparation research of meridian warming and pain relieving prescriptions, and provide reference for extract of other traditional Chinese medicine extracts that are difficult to spray drying.
MeSH term(s)	Chemistry, Pharmaceutical/methods ; Desiccation/methods ; Drugs, Chinese Herbal/chemistry ; Hot Temperature ; Medicine, Chinese Traditional ; Powders
Chemical Substances	Drugs, Chinese Herbal ; Powders
Language	Chinese
Publishing date	2020-04-01
Publishing country	China
Document type	Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.19540/j.cnki.cjcmm.20191001.304
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Article ; Online: Effects of modified Wenjing decoction on microcirculation in reproductive organs in rats with symptom patterns of cold coagulation and blood stasis.

Wang, Di / Cheng, Xiumei / Fang, Huimin / Ren, Yanqing / Li, Xinhua / Ren, Weiwei / Xue, Bing / Yang, Cairui

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan

2020 Volume 40, Issue 2, Page(s) 212–223

Abstract: ... Wenjing decoction (WJD) for the treatment of CCBS.: Methods: CCBS was induced in rats with an ice-water ...

Abstract	Objective: To investigate the relationship between symptom patterns of cold coagulation and blood stasis (CCBS) and microcirculation disturbance. In addition, we determined the efficacy of modified Wenjing decoction (WJD) for the treatment of CCBS. Methods: CCBS was induced in rats with an ice-water bath treatment. The ovarian function, microvascular and circulatory status of reproductive organs, and function of local microvascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs) were evaluated. Results: Ovarian dysfunction was observed in the rats with CCBS. It was characterized by the presence of an estrous cycle disorder and a decrease in reproductive hormone levels. Microvascular circulation disorders were associated with an imbalance in vasoconstriction, relaxation substances, nitric oxide, abnormal blood flow in whole blood, and decreased blood flow in the auricle and uterus. VECs were damaged, and VSMCs contracted and proliferated in ovarian and uterine tissues. Conclusion: Our findings suggest that the dysfunctional reproductive organs observed in gynecological CCBS may be closely related to the microcirculation disturbance of local tissues, microvascular contraction, and vascular remodeling. Modified WJD can be used to treat CCBS by improving microcirculation in reproductive organs.
MeSH term(s)	Animals ; Cold Temperature ; Cold-Shock Response/drug effects ; Drugs, Chinese Herbal/administration & dosage ; Female ; Humans ; Microcirculation/drug effects ; Ovary/drug effects ; Ovary/physiopathology ; Peripheral Vascular Diseases/drug therapy ; Peripheral Vascular Diseases/physiopathology ; Rats ; Rats, Sprague-Dawley ; Reproduction/drug effects ; Uterus/drug effects ; Uterus/physiopathology ; Vasoconstriction/drug effects
Chemical Substances	Drugs, Chinese Herbal
Language	English
Publishing date	2020-04-02
Publishing country	China
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	603186-9
ISSN	2589-451X ; 0254-6272 ; 0255-2922
ISSN (online)	2589-451X ; 0254-6272
ISSN	0255-2922
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Article ; Online: Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin

Li, Jialin / Liu, Xiaoyao / Jiang, Mei / Xu, Yantong / Wang, Chunguo / Hu, Yujie / Yang, Song / Gao, Jian / Wang, Weiling / Wang, Ting

Beijing University of Chinese Medicine Journal of Traditional Chinese Medical Sciences. 2023 July, v. 10, no. 3 p.296-309

2023

Abstract: To explore the effect and mechanism of action of the Wenjing Zhitong recipe (WZR) in primary ...

Abstract	To explore the effect and mechanism of action of the Wenjing Zhitong recipe (WZR) in primary dysmenorrhea (PD) treatment. Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was administrated. The rate of change in uterine contractility and the writhing test were used to evaluate the effects of WZR. The serum levels of prostaglandin F₂α (PGF₂α) and prostaglandin E₂ (PGE₂), and the activity of cyclooxygenase-2 (COX2) were detected by enzyme-linked immunosorbent assay (ELISA). The changes in phosphor-phospholipase C (pPLC/PLC), phosphor-protein kinase C (pPKC/PKC), and connexin 43 (CX43) expression were detected using immunohistochemistry and western blot. WZR significantly reduced the rate of change in uterine contractility and writhing times in the PD model. WZR treatment inhibited the enzymatic activity of COX2 and reduced the levels of PGF₂α, PGF₂α/PGE₂ and COX2 in the PD model. WZR also significantly reduced the expression of pPLC/PLC, pPKC/PKC and CX43. Targeting the inhibition of COX2 activity, caffeic acid and 1-acetyl-β-carboline were validated as the active ingredients in WZR responsible for reducing uterine contractions. WZR attenuated PD by inhibiting COX2 activity, downregulating PGF₂α/PGE₂ expression, and inhibiting the PKC signaling pathway.
Keywords	Western blotting ; blood serum ; caffeic acid ; connexins ; enzyme activity ; enzyme-linked immunosorbent assay ; estradiol ; immunohistochemistry ; mechanism of action ; models ; oxytocin ; prostaglandin synthase ; prostaglandins ; Wenjing zhitong recipe ; Primary dysmenorrhea ; Network pharmacology ; PGF2α ; PKC system
Language	English
Dates of publication	2023-07
Size	p. 296-309.
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	2834228-8
ISSN	2095-7548 ; 2589-0395
ISSN	2095-7548 ; 2589-0395
DOI	10.1016/j.jtcms.2023.06.012
Database	NAL-Catalogue (AGRICOLA)

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Article: Clinical Observation on the Improvement of Serum Sex Hormone and Ovarian Function in Premature Ovarian Failure Patients with Deficiency-Cold Syndrome by Combining Wenjing Decoction with Tiaobu Chongren Acupuncture and Moxibustion.

Yi, Anlun / Qin, Xianbing / Du, Zhiyin / Wang, Tingling / Liu, Fang

publication RETRACTED

Evidence-based complementary and alternative medicine : eCAM

2021 Volume 2021, Page(s) 3926822

Abstract: Objective: To explore the effect of Wenjing Decoction and Tiaobu Chongren acupuncture and ... 77.59%) (: Conclusion: On the basis of hormone replacement therapy, the combination of Wenjing ...

Abstract	Objective: To explore the effect of Wenjing Decoction and Tiaobu Chongren acupuncture and moxibustion therapy on serum sex hormones and ovarian function recovery in premature ovarian failure (POF) patients with deficiency-cold syndrome. Methods: From February 2018 to October 2019, 119 POI patients with deficiency-cold syndrome who met the selection criteria were randomly divided into the control group ( Result: The effective rate of the observation group (93.10%) was higher than that of the control group (77.59%) ( Conclusion: On the basis of hormone replacement therapy, the combination of Wenjing Decoction and Tiaobu Chongren acupuncture and moxibustion therapy is effective in treating POF patients with deficiency-cold syndrome, which can effectively regulate their serum sex hormone levels and promote the recovery of ovarian function.
Language	English
Publishing date	2021-09-11
Publishing country	United States
Document type	Journal Article ; Retracted Publication
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2021/3926822
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Article ; Online: Clinical Observation on the Improvement of Serum Sex Hormone and Ovarian Function in Premature Ovarian Failure Patients with Deficiency-Cold Syndrome by Combining Wenjing Decoction with Tiaobu Chongren Acupuncture and Moxibustion

Anlun Yi / Xianbing Qin / Zhiyin Du / Tingling Wang / Fang Liu

Evidence-Based Complementary and Alternative Medicine, Vol

2021 Volume 2021

Abstract: Objective. To explore the effect of Wenjing Decoction and Tiaobu Chongren acupuncture and ... and the observation group was treated with Wenjing Decoction and Tiaobu Chongren acupuncture and ... of hormone replacement therapy, the combination of Wenjing Decoction and Tiaobu Chongren acupuncture and moxibustion therapy is ...

Abstract	Objective. To explore the effect of Wenjing Decoction and Tiaobu Chongren acupuncture and moxibustion therapy on serum sex hormones and ovarian function recovery in premature ovarian failure (POF) patients with deficiency-cold syndrome. Methods. From February 2018 to October 2019, 119 POI patients with deficiency-cold syndrome who met the selection criteria were randomly divided into the control group (n = 59) and the observation group (n = 60). The control group was treated with hormone replacement therapy, and the observation group was treated with Wenjing Decoction and Tiaobu Chongren acupuncture and moxibustion therapy on the basis of the control group. Both groups were treated continuously for 3 months. After treatment, the clinical efficacy of the two groups was compared, the Chinese medicine syndrome score, serum estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), anti-mullerian hormone (AMH) and bilateral antral follicle count (AFC), mean ovarian volume, and endometrial thickness of the two groups before and after treatment were compared. Result. The effective rate of the observation group (93.10%) was higher than that of the control group (77.59%) (P<0.05). After treatment, the index scores of traditional Chinese medicine syndromes in two groups were lower than before (P<0.05), and the observation group was lower than the control group (P<0.05). The serum E2 and AMH levels in the two groups increased, while FSH and LH levels decreased compared with before treatment, and the observation group improved significantly compared with the control group (P<0.05). The bilateral AFC, mean ovarian volume, and endometrial thickness of the two groups increased compared with before treatment, and the observation group was higher than the control group (P<0.05). Conclusion. On the basis of hormone replacement therapy, the combination of Wenjing Decoction and Tiaobu Chongren acupuncture and moxibustion therapy is effective in treating POF patients with deficiency-cold syndrome, which can effectively regulate their serum sex hormone levels and promote the recovery of ovarian function.
Keywords	Other systems of medicine ; RZ201-999
Subject code	630
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Protein-Based Tools for Studying Neuromodulation.

Wang, Wenjing

ACS chemical biology

2024 Volume 19, Issue 4, Page(s) 788–797

Abstract: Neuromodulators play crucial roles in regulating neuronal activity and affecting various aspects of brain functions, including learning, memory, cognitive functions, emotional states, and pain modulation. In this Account, we describe our group's efforts ... ...

Abstract	Neuromodulators play crucial roles in regulating neuronal activity and affecting various aspects of brain functions, including learning, memory, cognitive functions, emotional states, and pain modulation. In this Account, we describe our group's efforts in designing sensors and tools for studying neuromodulation. Our lab focuses on developing new classes of integrators that can detect neuromodulators across the whole brain while leaving a mark for further imaging analysis at high spatial resolution. Our lab also designed chemical- and light-dependent protein switches for controlling peptide activity to potentially modulate the endogenous receptors of the neuromodulatory system in order to study the causal effects of selective neuronal pathways.
MeSH term(s)	Neurons/metabolism ; Brain/metabolism ; Neurotransmitter Agents/metabolism
Chemical Substances	Neurotransmitter Agents
Language	English
Publishing date	2024-04-06
Publishing country	United States
Document type	Journal Article ; Review
ISSN	1554-8937
ISSN (online)	1554-8937
DOI	10.1021/acschembio.4c00037
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Article ; Online: Application Evaluation of Fluorouracil Intraperitoneal Perfusion Chemotherapy in Combination with Intravenous Chemotherapy in Patients after Radical Resection of Colorectal Cancer.

Wang, Wenjing

Annali italiani di chirurgia

2024 Volume 95, Issue 1, Page(s) 98–104

Abstract: Background: Colorectal cancer stands as one of the most prevalent malignant tumors affecting the digestive tract, posing a significant threat to human health. Its incidence and fatality rates rank third and second, respectively, among malignant tumors. ... ...

Abstract	Background: Colorectal cancer stands as one of the most prevalent malignant tumors affecting the digestive tract, posing a significant threat to human health. Its incidence and fatality rates rank third and second, respectively, among malignant tumors. This study seeks to analyze the efficacy of combining fluorouracil intraperitoneal perfusion chemotherapy with intravenous chemotherapy in patients following radical resection of colorectal cancer. Methods: This retrospective study analyzed the medical records of 65 patients who underwent radical resection of colorectal cancer at the Affiliated Hospital of Shandong University of Traditional Chinese Medicine from January 2011 to January 2013. These patients were divided into two groups based on their treatment methods: the control group (CG, n = 31, receiving intravenous chemotherapy) and the observation group (OG, n = 32, receiving fluorouracil intraperitoneal perfusion chemotherapy + intravenous chemotherapy). After 6 cycles of treatment, the study compared clinical symptoms, Karnofsky score, body weight, adverse reactions, local recurrence, and liver metastasis between the two groups. Results: The OG demonstrated superior efficacy in controlling recurrence and metastasis compared to the CG (p < 0.05). However, there were no significant differences observed in clinical symptoms, quality of life, body weight, and drug safety between the two groups (p > 0.05). Conclusion: Intraperitoneal infusion chemotherapy with fluorouracil significantly impacts the control of recurrence and metastasis following radical resection of colorectal cancer. It also offers valuable references for developing clinical treatment protocols for these patients.
MeSH term(s)	Humans ; Fluorouracil ; Colorectal Neoplasms/drug therapy ; Colorectal Neoplasms/surgery ; Colorectal Neoplasms/pathology ; Quality of Life ; Retrospective Studies ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Perfusion ; Body Weight
Chemical Substances	Fluorouracil (U3P01618RT)
Language	English
Publishing date	2024-02-26
Publishing country	Italy
Document type	Journal Article
ZDB-ID	418927-9
ISSN	2239-253X ; 0003-469X
ISSN (online)	2239-253X
ISSN	0003-469X
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Article ; Online: A new strategy for the treatment of refractory ascites in patients with hepatitis B-related liver cirrhosis.

Xu, Wenjing / Wang, Gang

Asian journal of surgery

2024

Language	English
Publishing date	2024-03-26
Publishing country	Netherlands
Document type	Letter
ZDB-ID	1068461-x
ISSN	0219-3108 ; 1015-9584
ISSN (online)	0219-3108
ISSN	1015-9584
DOI	10.1016/j.asjsur.2024.03.134
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