Article ; Online: Promoter from Chinese hamster elongation factor-1a gene and Epstein-Barr virus terminal repeats concatemer fragment maintain stable high-level expression of recombinant proteins.
2023 Volume 11, Page(s) e16287
Abstract: Background: The Chinese hamster ovary (CHO) cell line is the main host for the high-titer production of therapeutic and diagnostic proteins in the biopharmaceutical industry. In most cases, plasmids for efficient protein expression in CHO cells are ... ...
Abstract | Background: The Chinese hamster ovary (CHO) cell line is the main host for the high-titer production of therapeutic and diagnostic proteins in the biopharmaceutical industry. In most cases, plasmids for efficient protein expression in CHO cells are based on the cytomegalovirus (CMV) promoter. The autologous Chinese hamster eukaryotic translation elongation factor 1 Methods: We made a series of deletions in the downstream flanking region of the EEF1A1 gene, and then in its upstream flanking region. The resulting plasmids, which coded for the enhanced green fluorescent protein (eGFP), were tested for the level of eGFP expression in the populations of stably transfected CHO DG44 cells and the stability of eGFP expression in the long-term culture in the absence of selection agents. Results: It was shown that in the presence of the EBVTR fragment, the entire downstream flanking region of the EEF1A1 gene could be excluded from the plasmid vector. Shortening of the upstream flanking region of the EEF1A1 gene to a length of 2.5 kbp also had no significant effect on the level of eGFP expression or long-term stability. The EBVTR fragment significantly increased expression stability for both the CMV and EEF1A1 promoter-based plasmids, and the expression level drop during the two-month culture was more significant for both CMV promoter-based plasmids. Conclusion: Target protein expression stability for the truncated plasmid, based on the EEF1A1 gene and EBVTR fragment, is sufficient for common biopharmaceutical applications, making these plasmid vectors a viable alternative to conventional CMV promoter-based vectors. |
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MeSH term(s) | Cricetinae ; Animals ; Cricetulus ; Herpesvirus 4, Human/genetics ; CHO Cells ; Epstein-Barr Virus Infections ; Recombinant Proteins/genetics ; Terminal Repeat Sequences ; Biological Products ; Cytomegalovirus Infections |
Chemical Substances | Recombinant Proteins ; Biological Products |
Language | English |
Publishing date | 2023-10-24 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 2703241-3 |
ISSN | 2167-8359 ; 2167-8359 |
ISSN (online) | 2167-8359 |
ISSN | 2167-8359 |
DOI | 10.7717/peerj.16287 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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