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Article ; Online: Oxidation of tyrosine: Antioxidant mechanism of l-DOPA disclosed.

Neyra Recky, Jael R / Serrano, Mariana P / Dántola, M Laura / Lorente, Carolina

2021 Volume 165, Page(s) 360–367

Abstract: ... dihydroxy-l-phenylalanine (commonly known as DOPA) and tyrosine dimers. Here, we report tyrosine recovery ...

Abstract	Tyrosine is an amino acid related to crucial physiological events and its oxidation, that produce beneficial or detrimental effects on biological systems, has been extensively studied. Degradation of tyrosine often begins with the loss of an electron in an electron transfer reaction in the presence of a suitable electron acceptor. The reaction is facilitated by excited states of the acceptor in photosensitized processes. Several products of tyrosine oxidation have been described, the main ones being 3,4-dihydroxy-l-phenylalanine (commonly known as DOPA) and tyrosine dimers. Here, we report tyrosine recovery from tyrosyl radical, after one-electron oxidation, in the presence of DOPA. We propose that under high oxidative stress the oxidation of tyrosine may be controlled, in part, by one of its oxidation products. Also, we present strong evidence of antioxidant action of DOPA by preventing tyrosine dimerization, one of the most serious oxidative protein modifications, and the origin of structural modifications leading to the loss of protein functionality.
MeSH term(s)	Antioxidants ; Levodopa ; Oxidation-Reduction ; Proteins/metabolism ; Tyrosine/metabolism
Chemical Substances	Antioxidants ; Proteins ; Tyrosine (42HK56048U) ; Levodopa (46627O600J)
Language	English
Publishing date	2021-01-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	807032-5
ISSN	1873-4596 ; 0891-5849
ISSN (online)	1873-4596
ISSN	0891-5849
DOI	10.1016/j.freeradbiomed.2021.01.037
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Article ; Online: Avoiding One-Electron Oxidation of Biomolecules by 3,4-Dihydroxy-L-Phenylalanine (DOPA)

Neyra Recky, Jael R / Dántola, M Laura / Lorente, Carolina

Photochemistry and photobiology

2022 Volume 99, Issue 2, Page(s) 570–579

Abstract: It has been proposed that 3,4-dihydroxy-L-phenylalanine (DOPA) has antioxidant properties, and ...

Abstract	It has been proposed that 3,4-dihydroxy-L-phenylalanine (DOPA) has antioxidant properties, and thus, the objective of this work was to evaluate the effect of adding DOPA during the photosensitized oxidation of tyrosine (Tyr), tryptophan (Trp), histidine (His), 2'-deoxyguanosine 5'-monophosphate (dGMP) and 2'-deoxyadenosine 5'-monophosphate (dAMP). It was observed that, upon pterin-photosensitized degradation of a given biomolecule in acidic aqueous solutions, the rate of the biomolecule consumption decreases due to the presence of DOPA. Although DOPA deactivates the excited states of pterin (Ptr), biomolecules do as well, being the bimolecular quenching constants in the diffusional control limit, indicating that DOPA antioxidant mechanism is not a simple deactivation of Ptr excited states. Laser flash photolysis experiments provide evidence of the formation of DOPA radical (DOPA(-H)
MeSH term(s)	Antioxidants/metabolism ; Electrons ; Oxidation-Reduction ; Pterins ; Levodopa/metabolism ; Photolysis
Chemical Substances	Antioxidants ; Pterins ; Levodopa (46627O600J)
Language	English
Publishing date	2022-10-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	123540-0
ISSN	1751-1097 ; 0031-8655
ISSN (online)	1751-1097
ISSN	0031-8655
DOI	10.1111/php.13718
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Article: Pre-Treatment of Fish By-Products to Optimize Feeding of Tenebrio molitor L. Larvae

Romero-Lorente, María-Ángeles / Fabrikov, Dmitri / Montes, Juan / Morote, Elvira / Barroso, Fernando G. / Vargas-García, María del Carmen / Varga, Ágnes Timea / Sánchez-Muros, María-José

Insects. 2022 Jan. 25, v. 13, no. 2

2022

Abstract: Fish discards are organic waste with high and good-quality protein levels, as well as a fatty acid profile rich in n−3 LCPUFAs, mainly eicosapentaenoic acid and docosahexaenoic acid. These discards can be used as food for Tenebrio molitor (Linnaeus, 1758) ...

Abstract	Fish discards are organic waste with high and good-quality protein levels, as well as a fatty acid profile rich in n−3 LCPUFAs, mainly eicosapentaenoic acid and docosahexaenoic acid. These discards can be used as food for Tenebrio molitor (Linnaeus, 1758) larvae, thus increasing the nutritional value of this insect. This study focused on increasing larval acceptance of fish through different pre-treatments of the diets provided, as well as increasing the accumulation of EPA and DHA in fish-fed larvae. Four different diets were prepared: control (broiler feed), DGF50: 50% dried ground fish (Pagellus bogaraveo, Brünnich, 1768) + 50% broiler feed, for different periods, FGF100: 100% fresh ground P. bogaraveo and DUF100: 100% dried whole unground P. bogaraveo. Growth, mortality, proximate composition, fatty acid profile and lipid nutritional indices were determined. Larvae fed with FGF100 displayed better results among treatments, doubling the initial weight, as well as increasing their protein level and decreasing fat levels. Regarding fatty acids, eicosapentaenoic acid and docosahexaenoic acid were only detected in larvae fed with a fish-based diet for a period longer than 5 days. These results show that pre-treatment of fish-based diets causes changes in the growth and compositional parameters of T. molitor larvae.
Keywords	Pagellus bogaraveo ; Tenebrio molitor ; diet ; docosahexaenoic acid ; eicosapentaenoic acid ; fatty acid composition ; fish ; insects ; mortality ; nutritive value ; organic wastes ; protein content ; proximate composition
Language	English
Dates of publication	2022-0125
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2662247-6
ISSN	2075-4450
ISSN	2075-4450
DOI	10.3390/insects13020125
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Pre-Treatment of Fish By-Products to Optimize Feeding of Tenebrio molitor L. Larvae

María-Ángeles Romero-Lorente / Dmitri Fabrikov / Juan Montes / Elvira Morote / Fernando G. Barroso / María del Carmen Vargas-García / Ágnes Timea Varga / María-José Sánchez-Muros

Insects, Vol 13, Iss 125, p

2022 Volume 125

Abstract: Fish discards are organic waste with high and good-quality protein levels, as well as a fatty acid profile rich in n −3 LCPUFAs, mainly eicosapentaenoic acid and docosahexaenoic acid. These discards can be used as food for Tenebrio molitor (Linnaeus, ... ...

Abstract	Fish discards are organic waste with high and good-quality protein levels, as well as a fatty acid profile rich in n −3 LCPUFAs, mainly eicosapentaenoic acid and docosahexaenoic acid. These discards can be used as food for Tenebrio molitor (Linnaeus, 1758) larvae, thus increasing the nutritional value of this insect. This study focused on increasing larval acceptance of fish through different pre-treatments of the diets provided, as well as increasing the accumulation of EPA and DHA in fish-fed larvae. Four different diets were prepared: control (broiler feed), DGF50: 50% dried ground fish ( Pagellus bogaraveo , Brünnich, 1768) + 50% broiler feed, for different periods, FGF100: 100% fresh ground P. bogaraveo and DUF100: 100% dried whole unground P. bogaraveo . Growth, mortality, proximate composition, fatty acid profile and lipid nutritional indices were determined. Larvae fed with FGF100 displayed better results among treatments, doubling the initial weight, as well as increasing their protein level and decreasing fat levels. Regarding fatty acids, eicosapentaenoic acid and docosahexaenoic acid were only detected in larvae fed with a fish-based diet for a period longer than 5 days. These results show that pre-treatment of fish-based diets causes changes in the growth and compositional parameters of T. molitor larvae.
Keywords	fatty acid ; insect meal ; EPA ; DHA ; fish discards ; Science ; Q
Subject code	590
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Sweet Cherry ( Prunus avium L.) Cracking during Development on the Tree and at Harvest

María Celeste Ruiz-Aracil / Juan Miguel Valverde / Jose Manuel Lorente-Mento / Alberto Carrión-Antolí / Salvador Castillo / Domingo Martínez-Romero / Fabián Guillén

Agriculture, Vol 13, Iss 1244, p

The Impact of Methyl Jasmonate on Four Different Growing Seasons

2023 Volume 1244

Abstract: Rainfall occurring during the developmental stages of sweet cherries on the tree can lead to significant preharvest losses, primarily due to fruit cracking. Certain cultivars exhibit a higher susceptibility to such losses, particularly when persistent ... ...

Abstract	Rainfall occurring during the developmental stages of sweet cherries on the tree can lead to significant preharvest losses, primarily due to fruit cracking. Certain cultivars exhibit a higher susceptibility to such losses, particularly when persistent rains coincide with advanced phenological stages. The current study aims to investigate the efficacy of preharvest methyl jasmonate (MeJA) applications at harvest and during distinct developmental ripening stages in mitigating sweet cherry cracking at harvest and on-tree ripening. Preharvest foliar applications of 0.5 mM MeJA were applied across various sweet cherry cultivars, including ‘Prime Giant’, ‘Early Lory’, ‘Sweetheart’, and ‘Staccato’. By conducting this experiment over four growing seasons, we evaluated the impact of this natural elicitor on the cracking tolerance of these cultivars. The results of our analysis indicate that MeJA preharvest treatments effectively reduce fruit cracking, enhancing abiotic stress tolerance. Additionally, these treatments induce a general delay in fruit ripening on the tree across the examined cultivars. This delayed ripening effect is reflected in several quality parameters at harvest, such as the fruit firmness, external colour, total soluble solids, and total acidity. These parameters in the MeJA-treated fruit were delayed compared to the control fruit or remained unaffected for the total acidity. Conversely, the MeJA treatments delayed the accumulation of total polyphenols, exhibiting a minimal impact on reducing pedicel browning. The enhanced tolerance to cracking and delayed ripening attributed to the MeJA preharvest treatments could be helpful for plot management. Consequently, these MeJA-based preharvest treatments hold potential as valuable tools in adapting to climate change and mitigating abiotic stress in sweet cherry.
Keywords	Prunus avium ; ripening stage ; preharvest ; cracking ; methyl jasmonate ; climate change ; Agriculture (General) ; S1-972
Subject code	571
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The genome of melon (Cucumis melo L.).

Garcia-Mas, Jordi / Benjak, Andrej / Sanseverino, Walter / Bourgeois, Michael / Mir, Gisela / González, Víctor M / Hénaff, Elizabeth / Câmara, Francisco / Cozzuto, Luca / Lowy, Ernesto / Alioto, Tyler / Capella-Gutiérrez, Salvador / Blanca, Jose / Cañizares, Joaquín / Ziarsolo, Pello / Gonzalez-Ibeas, Daniel / Rodríguez-Moreno, Luis / Droege, Marcus / Du, Lei /

Alvarez-Tejado, Miguel / Lorente-Galdos, Belen / Melé, Marta / Yang, Luming / Weng, Yiqun / Navarro, Arcadi / Marques-Bonet, Tomas / Aranda, Miguel A / Nuez, Fernando / Picó, Belén / Gabaldón, Toni / Roma, Guglielmo / Guigó, Roderic / Casacuberta, Josep M / Arús, Pere / Puigdomènech, Pere

Proceedings of the National Academy of Sciences of the United States of America

2012 Volume 109, Issue 29, Page(s) 11872–11877

Abstract: We report the genome sequence of melon, an important horticultural crop worldwide. We assembled 375 Mb of the double-haploid line DHL92, representing 83.3% of the estimated melon genome. We predicted 27,427 protein-coding genes, which we analyzed by ... ...

Abstract	We report the genome sequence of melon, an important horticultural crop worldwide. We assembled 375 Mb of the double-haploid line DHL92, representing 83.3% of the estimated melon genome. We predicted 27,427 protein-coding genes, which we analyzed by reconstructing 22,218 phylogenetic trees, allowing mapping of the orthology and paralogy relationships of sequenced plant genomes. We observed the absence of recent whole-genome duplications in the melon lineage since the ancient eudicot triplication, and our data suggest that transposon amplification may in part explain the increased size of the melon genome compared with the close relative cucumber. A low number of nucleotide-binding site-leucine-rich repeat disease resistance genes were annotated, suggesting the existence of specific defense mechanisms in this species. The DHL92 genome was compared with that of its parental lines allowing the quantification of sequence variability in the species. The use of the genome sequence in future investigations will facilitate the understanding of evolution of cucurbits and the improvement of breeding strategies.
MeSH term(s)	Base Sequence ; Biological Evolution ; Chromosome Mapping ; Chromosomes, Artificial, Bacterial/genetics ; Cucumis melo/genetics ; DNA Transposable Elements/genetics ; Disease Resistance/genetics ; Genes, Duplicate/genetics ; Genes, Plant/genetics ; Genome, Plant/genetics ; Genomics/methods ; Likelihood Functions ; Models, Genetic ; Molecular Sequence Annotation ; Molecular Sequence Data ; Phylogeny ; Sequence Alignment ; Sequence Analysis, DNA
Chemical Substances	DNA Transposable Elements
Language	English
Publishing date	2012-07-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.1205415109
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Article ; Online: Sweet Cherry (Prunus avium L.) Cracking during Development on the Tree and at Harvest: The Impact of Methyl Jasmonate on Four Different Growing Seasons

Ruiz-Aracil, María Celeste / Valverde, Juan Miguel / Lorente-Mento, Jose Manuel / Carrión-Antolí, Alberto / Castillo, Salvador / Martínez-Romero, Domingo / Guillén, Fabián

Agriculture. 2023 June 14, v. 13, no. 6

2023

Abstract	Rainfall occurring during the developmental stages of sweet cherries on the tree can lead to significant preharvest losses, primarily due to fruit cracking. Certain cultivars exhibit a higher susceptibility to such losses, particularly when persistent rains coincide with advanced phenological stages. The current study aims to investigate the efficacy of preharvest methyl jasmonate (MeJA) applications at harvest and during distinct developmental ripening stages in mitigating sweet cherry cracking at harvest and on-tree ripening. Preharvest foliar applications of 0.5 mM MeJA were applied across various sweet cherry cultivars, including ‘Prime Giant’, ‘Early Lory’, ‘Sweetheart’, and ‘Staccato’. By conducting this experiment over four growing seasons, we evaluated the impact of this natural elicitor on the cracking tolerance of these cultivars. The results of our analysis indicate that MeJA preharvest treatments effectively reduce fruit cracking, enhancing abiotic stress tolerance. Additionally, these treatments induce a general delay in fruit ripening on the tree across the examined cultivars. This delayed ripening effect is reflected in several quality parameters at harvest, such as the fruit firmness, external colour, total soluble solids, and total acidity. These parameters in the MeJA-treated fruit were delayed compared to the control fruit or remained unaffected for the total acidity. Conversely, the MeJA treatments delayed the accumulation of total polyphenols, exhibiting a minimal impact on reducing pedicel browning. The enhanced tolerance to cracking and delayed ripening attributed to the MeJA preharvest treatments could be helpful for plot management. Consequently, these MeJA-based preharvest treatments hold potential as valuable tools in adapting to climate change and mitigating abiotic stress in sweet cherry.
Keywords	Prunus avium ; abiotic stress ; acidity ; agriculture ; climate change ; color ; cultivars ; elicitors ; fruit quality ; fruits ; methyl jasmonate ; pedicel ; phenology ; polyphenols ; rain ; stress tolerance ; trees
Language	English
Dates of publication	2023-0614
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2651678-0
ISSN	2077-0472
ISSN	2077-0472
DOI	10.3390/agriculture13061244
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: EFECTO DE LA MADUREZ EN LA CRIOCONSERVACIÓN DE SEMILLAS DE Nicotiana tabacum L.

Juan L. Pérez-Rodríguez / Gilberto Torrecilla Guerra / Otilio Ruiz Padrón / René C. Rodríguez Escriba / Gustavo Y. Lorente González / Marcos E. Martínez Montero / Justo L. González Olmedo

Cultivos Tropicales, Vol 37, Pp 99-

2016 Volume 105

Abstract: ... en la crioconservación de semillas de Nicotiana tabacum L. cv Sancti Spíritus 96 (SS-96). El estudio se realizó ...

Abstract	La presente investigación tuvo como objetivo determinar el efecto de la madurez en la crioconservación de semillas de Nicotiana tabacum L. cv Sancti Spíritus 96 (SS-96). El estudio se realizó en la Estación experimental del tabaco de Cabaiguán, Sancti Spíritus, perteneciente al Instituto de Investigaciones del Tabaco, entre los años 2012 y 2014. Las semillas de SS-96 se colectaron a los 14, 21, 28, 35, 42, 49 y 56 días después de la antesis (DDA) y se les determinó masa fresca y masa seca de 1000 semillas, porcentaje de humedad (base masa fresca) y potencia germinativa (PG), además de evaluar su tolerancia a la desecación. Al mismo tiempo, semillas de cada día de colecta se conservaron a 5 ºC o en nitrógeno líquido y, transcurridos 30 días, les fue evaluada su PG y se sometieron a las pruebas de envejecimiento acelerado y pérdida de electrólitos. Durante el desarrollo de la investigación se determinó que la madurez fisiológica de las semillas de SS-96 se alcanza a los 29,6 DDA, con un contenido de agua de 30,6 % y masa seca de 1000 semillas de 81,2 mg. Se estableció 35 DDA como el momento idóneo para la colecta de semillas en este cultivar. Una colecta antes de esta fecha provoca una disminución en la tolerancia a la desecación producto de la inmadurez de las semillas; una recolección posterior conlleva a la pérdida de vigor debido a la mayor exposición de las semillas a las condiciones ambientales.
Keywords	Agriculture (General) ; S1-972 ; Agriculture ; S
Language	Spanish
Publishing date	2016-01-01T00:00:00Z
Publisher	Instituto Nacional de Ciencias Agrícolas
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Chemical guide parameters for Spanish lemon (Citrus limon (L.) Burm.) juices.

Lorente, José / Vegara, Salud / Martí, Nuria / Ibarz, Albert / Coll, Luís / Hernández, Julio / Valero, Manuel / Saura, Domingo

Food chemistry

2014 Volume 162, Page(s) 186–191

Abstract: ... juice, titratable acidity was 52.4 g/L, being the citric acid the main component. Glucose, fructose and ... sucrose concentrations were 7.9, 7.3 and 4.5 g/L, respectively. Predominant mineral was potassium (1264 ... 2mg/L), followed by phosphorous (306 mg/L), calcium (112 mg/L) and magnesium (92.6 mg/L). Hesperidin ...

Abstract	To contribute for setting reference guideline for commercial juice extracted from the Spanish lemon varieties, chemical composition of 92 direct and 92 reconstituted samples were investigated. In direct lemon juice, titratable acidity was 52.4 g/L, being the citric acid the main component. Glucose, fructose and sucrose concentrations were 7.9, 7.3 and 4.5 g/L, respectively. Predominant mineral was potassium (1264.2mg/L), followed by phosphorous (306 mg/L), calcium (112 mg/L) and magnesium (92.6 mg/L). Hesperidin ranged from 257 to 484.8 mg/L, while water soluble pectins varied between 164.8 and 550 mg/L. Similar values were obtained in reconstituted lemon juice. There are different parameters that did not reach or exceeded the limits proposed by the European Association of the Industry of Juices and Nectars. These levels should be taken into account to modify the present reference guideline and that Spanish lemon juices are not discarded for to have lower or bigger values.
MeSH term(s)	Beverages/analysis ; Citrus/chemistry ; Food Additives/analysis ; Fruit/chemistry ; Spain
Chemical Substances	Food Additives
Language	English
Publishing date	2014-11-01
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2014.04.042
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Book ; Online: Production and Export of Green Beans (Phaseolus vulgaris L.) ; Producción y Exportación de Ejote Francés (Phaseolus vulgaris L.)

Hernández Ocampo, Gustavo Adolfo / Morales Rivera, Rommel Noé / Valdivia Lorente, Rodolfo Ramón / Castillo, Edwin / Palma, Santos

2019

Abstract: This publication is the product of a participatory capitalization process facilitated by the International Center for Tropical Agriculture (CIAT) for the Program for Rural Enterprise Management, Health, and the environment (PROGRESA). This report aims to ...

Abstract	This publication is the product of a participatory capitalization process facilitated by the International Center for Tropical Agriculture (CIAT) for the Program for Rural Enterprise Management, Health, and the environment (PROGRESA). This report aims to share the stakes, risks, lessons learned, and outcome of PROGRESA’s initiative to promote and assist Nicaraguan vegetable producers in the production and export of green beans.
Keywords	knowledge management ; supply chain ; sustainability ; markets ; beans ; gender
Language	English
Publishing date	2019-04-15T14:48:43Z
Publisher	Catholic Relief Services
Publishing country	fr
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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