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Article ; Online: Precision irrigation trends and perspectives

Alice da Silva Gundim / Verônica Gaspar Martins Leite de Melo / Rubens Duarte Coelho / Janderson Pedro da Silva / Marcos Paulo Alves da Rocha / Ana Carolina Ferreira França / Ana Michele Pereira da Conceição

Ciência Rural, Vol 53, Iss

a review

2023 Volume 8

Abstract: ABSTRACT: In recent decades, research on precision irrigation driven by climate change has developed a multitude of strategies, methods and technologies to reduce water consumption in irrigation projects and to adapt to the increasing occurrence of water ...

Abstract	ABSTRACT: In recent decades, research on precision irrigation driven by climate change has developed a multitude of strategies, methods and technologies to reduce water consumption in irrigation projects and to adapt to the increasing occurrence of water scarcity, agricultural droughts and competition between agricultural and industrial sectors for the use of water. In this context, the adoption of water-saving and application practices implies a multidisciplinary approach to accurately quantify the water needs of crops under different water availability and management practices. Thus, this review article presented a review of technologies and new trends in the context of precision irrigation, future perspectives and critically analyze notions and means to maintain high levels of land and water productivity, which minimize irrational water consumption at the field level.
Keywords	precision agriculture ; water management ; water saving ; irrigation technologies ; Agriculture ; S ; Agriculture (General) ; S1-972
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	Universidade Federal de Santa Maria
Document type	Article ; Online
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Article ; Online: Human papillomavirus infection and oral squamous cell carcinoma - a systematic review.

Melo, Bernardo Augusto de Carvalho / Vilar, Luisa Gallo / Oliveira, Natália Rodrigues de / Lima, Priscila Oliveira de / Pinheiro, Melina de Barros / Domingueti, Caroline Pereira / Pereira, Michele Conceição

Brazilian journal of otorhinolaryngology

2020 Volume 87, Issue 3, Page(s) 346–352

Abstract: Introduction: The association between uterine cervix and anogenital carcinomas and human papillomavirus, HPV, is well established, however the involvement of this virus in the development of oral squamous cell carcinomas remains controversial.: ... ...

Abstract	Introduction: The association between uterine cervix and anogenital carcinomas and human papillomavirus, HPV, is well established, however the involvement of this virus in the development of oral squamous cell carcinomas remains controversial. Objectives: To evaluate the relationship between HPV infection and oral squamous cell carcinomas, and to estimate the incidence of this infection in these patients. Methods: Four electronic databases were searched to find studies that met the following inclusion criteria: i) performed in humans; ii) were cohort, case-control or cross-sectional; iii) assessed the HPV oncogenic activity by the E6 and E7 mRNA; iv) included primary oral squamous cell carcinomas which; v) diagnosis had been confirmed by biopsy. Information about the country; study period; sample obtainment; sites of oral squamous cell carcinomas; number, gender and age range of the population; the prevalence of HPV infection and subtypes detected; use of tobacco or alcohol and oral sex practice were extracted. The methodological quality of included articles was assessed using 14 criteria. Results: The search strategy retrieved 2129 articles. Assessment of the full text was done for 626 articles, but five were included. The total of participants included was 383, most of them male with mean age between 51.0 and 63.5 years old. Seventeen patients were HPV/mRNA-positive, being the subtypes 16 and 18 detected more frequently. Nine of the HPV/mRNA-positive oral squamous cell carcinomas occurred on the tongue. The quality score average of included articles was five points. Conclusions: Among the 383 oral squamous cell carcinoma patients included, 17 (4.4%) were HPV/mRNA-positive, nevertheless it was not possible to assess if HPV infection was associated with oral squamous cell carcinomas because none of the studies included was longitudinal and cross-sectional investigations do not have control group.
MeSH term(s)	Carcinoma, Squamous Cell/epidemiology ; Cross-Sectional Studies ; DNA, Viral ; Female ; Head and Neck Neoplasms ; Humans ; Male ; Middle Aged ; Mouth Neoplasms/epidemiology ; Papillomaviridae/genetics ; Papillomavirus Infections/epidemiology ; Squamous Cell Carcinoma of Head and Neck/epidemiology
Chemical Substances	DNA, Viral
Language	English
Publishing date	2020-11-29
Publishing country	Brazil
Document type	Journal Article ; Review ; Systematic Review
ZDB-ID	2428110-4
ISSN	1808-8686 ; 1808-8694
ISSN (online)	1808-8686
ISSN	1808-8694
DOI	10.1016/j.bjorl.2020.10.017
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Article ; Online: Partial purification of glucose-6-phosphate dehydrogenase by aqueous two-phase poly(ethyleneglycol)/phosphate systems Purificação parcial de glucose-6-fosfato desidrogenase por sistemas de duas fases aquosas poli (etilenoglicol)/fosfato

Marcela Zanella Ribeiro / Daniel Pereira Silva / Michele Vitolo / Inês Conceição Roberto / Adalberto Pessoa-Jr

Brazilian Journal of Microbiology, Vol 38, Iss 1, Pp 78-

2007 Volume 83

Abstract: Glucose-6-phosphate dehydrogenase (G6PDH) is an important enzyme used in biochemical and medical studies and in several analytical methods that have industrial and commercial application. This work evaluated the extraction of G6PDH in aqueous two-phase ... ...

Abstract	Glucose-6-phosphate dehydrogenase (G6PDH) is an important enzyme used in biochemical and medical studies and in several analytical methods that have industrial and commercial application. This work evaluated the extraction of G6PDH in aqueous two-phase system (ATPS) of poly(ethyleneglycol) (PEG)/phosphate buffer, using as enzyme source a medium prepared through commercial baker's yeast disruption. Firstly, the effects of PEG molar mass on the enzyme partition and of homogenization and rest on the system equilibrium were investigated. Afterwards, several ATPS were prepared using statistical analysis (2² factorial design). The results, including kinetic and thermodynamic parameters for the G6PDH activity, showed partial purification of this enzyme in ATPS composed of 17.5% (w/w) PEG400 and 15.0% (w/w) phosphate. A high enzymatic recovery value (97.7%), a high partition coefficient (351), and an acceptable purification factor (2.28 times higher than in cell homogenate) were attained from the top phase. So, it was possible to attain an effective enzyme pre-purification by separating some contaminants with a simple method such as liquid-liquid extraction in aqueous two-phase systems (ATPS). Glicose-6-fosfato desidrogenase (G6PDH) é uma importante enzima usada em estudos bioquímicos e médicos, bem como em diversos métodos analíticos com aplicação comercial e industrial. Neste trabalho foi avaliado a extração da G6PDH em sistemas de duas fases aquosas (ATPS) constituídos por poli(etilenoglicol) (PEG)/tampão fosfato, usando como fonte de enzima um meio preparado por rompimento de leveduras de panificação comercial. Inicialmente foram investigados os efeitos da massa molar do PEG na partição da enzima e da homogeneização e repouso no equilíbrio do sistema. Na sequência, diversos ATPS foram preparados usando análise estatística (planejamento fatorial 2²). Os resultados, incluindo parâmetros cinéticos e termodinâmicos para a atividade da G6PDH, indicaram parcial purificação desta enzima em ATPS constituídos por 17,5% (p/p) PEG400 e 15,0% (p/p) fosfato. Um alto valor de recuperação enzimática (97,7%), um alto coeficiente de partição (351), e um fator de purificação aceitável (2,28 vezes maior que em homogenato celular) foram obtidos na fase superior do sistema. Assim, foi possível alcançar uma pré-purificação eficaz da enzima separando alguns contaminadores aplicando um método simples tal como a extração líquido-líquido em sistemas bifásicos (ATPS).
Keywords	sistema de duas fases aquosas ; glicose-6-fosfato desidrogenase ; extração líquido-líquido ; Saccharomyces cerevisiae ; aqueous two-phase systems ; glucose-6-phosphate dehydrogenase ; liquid-liquid extraction ; Microbiology ; QR1-502 ; Science ; Q ; DOAJ:Microbiology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
Language	English
Publishing date	2007-03-01T00:00:00Z
Publisher	Sociedade Brasileira de Microbiologia
Document type	Article ; Online
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Article ; Online: The role of eosinophils and eosinophil cationic protein in oral cancer: a review.

Pereira, Michele Conceição / Oliveira, Denise Tostes / Kowalski, Luiz Paulo

Archives of oral biology

2011 Volume 56, Issue 4, Page(s) 353–358

Abstract: Eosinophils are multifunctional leukocytes implicated in the pathogenesis of numerous inflammatory processes, such as allergies and parasitic infections. Increased number of these cells has been described in many human cancers, including oral squamous ... ...

Abstract	Eosinophils are multifunctional leukocytes implicated in the pathogenesis of numerous inflammatory processes, such as allergies and parasitic infections. Increased number of these cells has been described in many human cancers, including oral squamous cell carcinoma, and its presence related to favourable as well as unfavourable prognosis. Although the exact role of eosinophils in tumours is not yet defined, the anti-tumour activity of these rare granulocytes has been associated with the release of their cytotoxic proteins, especially the eosinophil cationic protein, a single polypeptide chain with a molecular mass ranging from 15 to 22kDa encoded by the RNSE3 gene located on chromosome 14q11.2. Functional studies have implicated ECP in numerous processes, such as tissue remodelling in allergic inflammation; however its most striking function is the cytotoxic activity. The aim of this review is to summarise the role and functions of eosinophils and their granule-derived products in oral malignant tumours and the clinical value of the tumour-associated tissue eosinophilia for patients' prognosis.
MeSH term(s)	Eosinophil Cationic Protein/immunology ; Eosinophilia/blood ; Eosinophilia/complications ; Eosinophilia/immunology ; Eosinophils/immunology ; Humans ; Mouth Neoplasms/blood ; Mouth Neoplasms/complications ; Mouth Neoplasms/immunology
Chemical Substances	Eosinophil Cationic Protein (EC 3.1.27.-)
Language	English
Publishing date	2011-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	80227-x
ISSN	1879-1506 ; 0003-9969
ISSN (online)	1879-1506
ISSN	0003-9969
DOI	10.1016/j.archoralbio.2010.10.015
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Article ; Online: Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis.

Finger, Paula Fonseca / Pepe, Michele Soares / Dummer, Luana Alves / Magalhães, Carolina Georg / de Castro, Clarissa Caetano / de Oliveira Hübner, Silvia / Leite, Fábio Pereira Leivas / Ritterbusch, Giseli Aparecida / Esteves, Paulo Augusto / Conceição, Fabricio Rochedo

Virology journal

2018 Volume 15, Issue 1, Page(s) 189

Abstract: Background: The avian infectious bronchitis virus (IBV) remains a significant source of loss in the poultry industry and early diagnosis is required to prevent the disease from spreading. This study examined the combined use of an ELISA and Western blot ...

Abstract	Background: The avian infectious bronchitis virus (IBV) remains a significant source of loss in the poultry industry and early diagnosis is required to prevent the disease from spreading. This study examined the combined use of an ELISA and Western blot (WB) to detect antibodies against the nucleocapsid protein (N) of IBV. The coding sequence for N was amplified by RT-PCR and expressed in Escherichia coli. A soluble recombinant N protein (rN) of approximately 50 kDa was obtained. A total of 389 sera were tested against the rN in ELISA and the results were compared with those of the commercial IDEXX IBV Ab test. ELISA-rN achieved a 90.34% sensitivity and 90.16% specificity. WB confirmed all false negative sera in ELISA-rN or IDEXX test as truly positive. The current study indicate that the combined use of rN in ELISA and WB is a powerful tool for the immunodiagnosis of avian infectious bronchitis. Methods: Constructed recombinant pAE/n expression vectors were used to transform E. coli BL21(DE3) Star competent cells (Invitrogen). The rN of infectious bronchitis virus was purified by affinity chromatography using HisTrap HP 1 mL columns pre-packed with pre-charged Ni Sepharose in the ÄKTAprime Automated Liquid Chromatography system (GE Healthcare). A total of 389 serum samples from chickens were used to develop and evaluate the ELISA-rN test. To standardize the indirect ELISA development, serum dilutions (1:100, 1:200 and 1:400) and different concentrations of purified rN antigen (50, 100 and 200 ng/well) were tested. Positive and negative sera for IBV were used as controls. The results were compared with those obtained from a commercial kit. Serum samples scored as negative with the commercial kit but as positive with the ELISA-rN were further analysed by Western blot analyses using the rN protein as an antigen. The results of the ELISA-rN were compared to the commercial kit results using receiver-operating characteristics curves, area under the curve, and confidence intervals with the software GraphPad Prism version 6.0 for Windows (GraphPad Software, USA). Results: The expected cDNA fragment of approximately 1240 bp was successfully amplified by PCR using primers designed to select for the coding region of the N protein. The rN was expressed as a soluble protein to avoid the refolding steps and, after purification a yield of 10 mg/L of rN was obtained. The SDS-PAGE results demonstrated the presence of two distinct bands that had a molecular mass of approximately 45 and 50 KDa. Out of 244 sera that scored positive in the commercial ELISA IDEXX IBV Ab Test, 220 were also positive in the ELISA-rN, yielding an ELISA-rN test sensitivity of 90.16%. Out of 145 sera that scored negative in the IDEXX IBV Ab Test, 131 also scored negative in the ELISA-rN, indicating a specificity of 90.34%. Sera that tested negative in the ELISA-rN and positive in the commercial test also reacted with the rN protein in Western blot. Conclusions: The association between the ELISA and Western blot techniques developed in this study with a subunit of IBV (rN) were able to detect antibodies that the commercial ELISA did not detect suggesting that the ELISA-rN has greater sensitivity.
MeSH term(s)	Animals ; Antibodies, Viral/blood ; Antigens, Viral/immunology ; Blotting, Western/methods ; Chickens ; Coronavirus Infections/diagnosis ; Coronavirus Infections/immunology ; Coronavirus Infections/veterinary ; Early Diagnosis ; Enzyme-Linked Immunosorbent Assay/methods ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Immunologic Tests/methods ; Infectious bronchitis virus/genetics ; Infectious bronchitis virus/metabolism ; Nucleocapsid Proteins/genetics ; Nucleocapsid Proteins/immunology ; Poultry Diseases/diagnosis ; Poultry Diseases/virology ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Sensitivity and Specificity
Chemical Substances	Antibodies, Viral ; Antigens, Viral ; Nucleocapsid Proteins ; Recombinant Proteins ; nucleocapsid protein, Coronavirus
Keywords	covid19
Language	English
Publishing date	2018-12-12
Publishing country	England
Document type	Journal Article
ISSN	1743-422X
ISSN (online)	1743-422X
DOI	10.1186/s12985-018-1096-2
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Article ; Online: Sensitivity and specificity of recombinant proteins in Toxocara spp. for serodiagnosis in humans: Differences in adult and child populations.

Santos, Lucas Moreira Dos / Magalhães, Carolina Georg / Telmo, Paula de Lima / Cerqueira, Michele Pepe / Donassolo, Rafael Amaral / Leite, Fábio Pereira Leivas / Elefant, Guita Rubinsky / Avila, Luciana Farias da Costa / Scaini, Carlos James / Moreira, Ângela Nunes / Conceição, Fabricio Rochedo

PloS one

2018 Volume 13, Issue 12, Page(s) e0208991

Abstract: Toxocariasis is a neglected zoonosis that affects children and adults. Recombinant proteins have been widely investigated for diagnosis, achieving high sensitivity and specificity in an overall population; however, little is known about age as a factor ... ...

Abstract	Toxocariasis is a neglected zoonosis that affects children and adults. Recombinant proteins have been widely investigated for diagnosis, achieving high sensitivity and specificity in an overall population; however, little is known about age as a factor in its application. This study aims to investigate the diagnostic potential of Toxocara canis TES-30 and TES-120 recombinant proteins in humans, differentiating between its performance in children and adults. Serum samples collected from children and adults seropositive to Toxocara spp. were tested with indirect ELISA using T. canis TES-30 and TES-120 recombinant proteins produced in Escherichia coli. While rTES-30 sensitivity was not affected by age (81.8% in children and 87% in adults), rTES-120 sensitivity severely decreased in children to only 63.6%, down from 95.7% in adults. Furthermore, the sensitivity of rTES-30 increased to 97.8% after Western blotting confirmation. High specificity (>94%) against other geohelminths was reported for both recombinant proteins. Our study favors the use of rTES-30 with total IgG as the primary antibody in an indirect ELISA assay as a tool for epidemiological human studies.
MeSH term(s)	Adult ; Animals ; Child ; Child, Preschool ; Costs and Cost Analysis ; Humans ; Infant ; Prognosis ; Recombinant Proteins/metabolism ; Sensitivity and Specificity ; Serologic Tests/economics ; Serologic Tests/methods ; Time Factors ; Toxocara
Chemical Substances	Recombinant Proteins
Language	English
Publishing date	2018-12-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0208991
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Article ; Online: Hypoxic condition and prognosis in oral squamous cell carcinoma.

DE Lima, Priscila Oliveira / Jorge, Camila Cassani / Oliveira, Denise Tostes / Pereira, Michele Conceição

Anticancer research

2014 Volume 34, Issue 2, Page(s) 605–612

Abstract: Oral squamous cell carcinomas (OSCCs) have the potential for rapid and unlimited growth. Therefore, hypoxic tissue areas are common in these malignant tumours and contribute to cancer progression, resistance to therapy and poor outcome. Out of all ... ...

Abstract	Oral squamous cell carcinomas (OSCCs) have the potential for rapid and unlimited growth. Therefore, hypoxic tissue areas are common in these malignant tumours and contribute to cancer progression, resistance to therapy and poor outcome. Out of all proteins induced by hypoxia, hypoxia-inducible factors (HIFs) and their target genes have been most extensively studied. HIF1 is a heterodimeric transcriptional complex that functions as the main regulator of systemic and cellular oxygen homeostasis; it is composed of HIF1α and HF1β subunits. At physiological concentrations of oxygen, prolyl hydroxylases (PHDs) modify HIF1α and prepare it for proteasomal degradation. In hypoxia, PHDs are inhibited and HIF1α dimerises with HIF1β to form HIF1, which is responsible for the activation of several genes involved in multiple aspects of tumor biology. Among these genes, vascular endothelial growth factor (VEGF) is essential as a regulatory gene of angiogenesis in the adaptation to a hypoxic microenvironment. Previous studies have shown the correlation between HIF1α and VEGF in OSCC and high levels of HIF1α expression appear to predict a poor prognosis. The purpose of the present article is to review the hypoxic condition in OSCC and its correlation with prognosis.
MeSH term(s)	Animals ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/pathology ; Cell Hypoxia/physiology ; Disease Progression ; Humans ; Mouth Neoplasms/metabolism ; Mouth Neoplasms/pathology
Language	English
Publishing date	2014-02
Publishing country	Greece
Document type	Journal Article ; Review
ZDB-ID	604549-2
ISSN	1791-7530 ; 0250-7005
ISSN (online)	1791-7530
ISSN	0250-7005
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Article ; Online: Cytotoxic Activities of Eosinophil Cationic Protein and Eosinophil-derived Neurotoxin: In Silico Analysis.

De Oliveira, Paula Cristina Rabelo / De Oliveira Lopes, Débora / Do Vale Coelho, Ivan Evangelista / Pereira, Michele Conceição

Cancer genomics & proteomics

2015 Volume 12, Issue 6, Page(s) 397–402

Abstract: Background: Eosinophil cationic protein (ECP) and eosinophil derived-neurotoxin (EDN) are homologous ribonuclease (RNAse) A family proteins. The objective of the present study was to in silico characterize ECP and EDN with respect to their cytotoxic ... ...

Abstract	Background: Eosinophil cationic protein (ECP) and eosinophil derived-neurotoxin (EDN) are homologous ribonuclease (RNAse) A family proteins. The objective of the present study was to in silico characterize ECP and EDN with respect to their cytotoxic activities. Materials and methods: Structural, physicochemical, and conserved domain characterizations were carried-out using open-source software, such as InterProScan, NetOGlyc, NetPhos and Discovery Studio 3.1. Results: The proteins did not have atypical conserved domains. EDN had a greater number of glutamine amino acid residues, whereas ECP had a predominance of arginine. ECP had four possible N-glycosylation, three O-glycosylation and four phosphorylation sites. EDN had five putative N-glycosylation, three phosphorylation and no O-glycosylation sites. Conclusion: The greater cationicity of ECP may be related to its higher cytotoxicity and to the fact that the varying post-translational modification profiles can generate functional differences from structural alteration. In vivo and in vitro studies need to be performed in order to confirm these predictions.
MeSH term(s)	Arginine/chemistry ; Computational Biology ; Databases, Protein ; Eosinophil Cationic Protein/metabolism ; Eosinophil-Derived Neurotoxin/metabolism ; Gene Expression Regulation ; Glutamine/chemistry ; Glycosylation ; Humans ; Protein Processing, Post-Translational ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Ribonuclease, Pancreatic/chemistry ; Software
Chemical Substances	Glutamine (0RH81L854J) ; Arginine (94ZLA3W45F) ; Eosinophil-Derived Neurotoxin (EC 3.1.-) ; Eosinophil Cationic Protein (EC 3.1.27.-) ; Ribonuclease, Pancreatic (EC 3.1.27.5)
Language	English
Publishing date	2015-11
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2144517-5
ISSN	1790-6245 ; 1109-6535
ISSN (online)	1790-6245
ISSN	1109-6535
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Article ; Online: Influence of environmental contamination on pregnancy outcomes.

Guimarães, Mariana Tavares / Cunha, Michele Granato / Carvalho, Daniele Pena / Ribeiro, Tatyana Sampaio / Martins, Lourdes Conceição / Braga, Alfésio Luís Ferreira / Pereira, Luiz Alberto Amador

Environmental science and pollution research international

2015 Volume 22, Issue 19, Page(s) 14950–14959

Abstract: This study aims to compare pregnancy outcomes in four contaminated areas to those observed in a non-contaminated area of similar socioeconomic status. A cross-sectional study was carried out. A structured and pre-tested questionnaire was administered to ... ...

Abstract	This study aims to compare pregnancy outcomes in four contaminated areas to those observed in a non-contaminated area of similar socioeconomic status. A cross-sectional study was carried out. A structured and pre-tested questionnaire was administered to population-based samples of 788-920 families in each of the five studied areas. The exposure assessment used was an ecological measure. Using logistic regression, odds of several pregnancies outcomes (pregnancy occurrence, miscarriage, stillbirth, prematurity, low birth weight, congenital malformation, and multiple births) were estimated after adjustment for potential confounders such as socioeconomic, demographic, and substance abuse factors. We adopted a statistical significance level of 5 %. In three of the four exposed areas, pregnancy occurrence was reduced in comparison to the control area (Area 2, odds ratio (OR) = 0.68, 95 % CI = 0.54-0.86; Area 3, OR = 0.76, 95 % CI = 0.60-0.97; Area 4, OR = 0.71, 95 % CI = 0.56-0.90). Also, a significantly increased odds of miscarriage for living in Area 3 (OR = 1.83, 95 % CI = 1.07-3.12) was found. The other pregnancy outcomes were not significantly elevated in the exposed areas. In conclusion, this study shows evidence of reduced pregnancy occurrence and increased miscarriage occurrence in some of the contaminated areas, compared to the control area.
MeSH term(s)	Adolescent ; Adult ; Cross-Sectional Studies ; Environmental Pollutants/toxicity ; Female ; Humans ; Infant, Newborn ; Logistic Models ; Middle Aged ; Odds Ratio ; Pregnancy ; Pregnancy Outcome ; Young Adult
Chemical Substances	Environmental Pollutants
Language	English
Publishing date	2015-10
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-015-4704-3
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Article ; Online: Immunohistochemical profile of canalicular adenoma of the upper lip: a case report.

Pereira, Michele Conceição / Pereira, Alessandro Antônio Costa / Hanemann, João Adolfo Costa

Medicina oral, patologia oral y cirugia bucal

2007 Volume 12, Issue 1, Page(s) E1–3

Abstract: Canalicular adenoma is an uncommon benign salivary gland neoplasm that has a marked predilection for occurrence in the upper lip. It is composed of columnar cells arranged in branching and interconnecting cords of single or double cell thick rows. This ... ...

Abstract	Canalicular adenoma is an uncommon benign salivary gland neoplasm that has a marked predilection for occurrence in the upper lip. It is composed of columnar cells arranged in branching and interconnecting cords of single or double cell thick rows. This tumor has an excellent prognosis after conservative surgical treatment in all locations. In the present report we describe, using immunohistochemistry, the expression of cytokeratins (CK), S-100 protein and EMA in a canalicular adenoma that arose in the upper lip of a 55-year-old female. Cells of the canalicular adenoma showed an immunohistochemical profile that indicates an excretory duct origin: most of these cells positively expressed AE1/AE3 cytokeratins and S100 protein. A comparison of the immunohistochemical features of canalicular adenoma with other salivary gland neoplasms that share similar histological features is discussed.
MeSH term(s)	Adenoma/pathology ; Female ; Humans ; Immunohistochemistry ; Lip Neoplasms/pathology ; Middle Aged
Language	English
Publishing date	2007-01-01
Publishing country	Spain
Document type	Case Reports ; Journal Article
ZDB-ID	2171573-7
ISSN	1698-6946 ; 1698-4447
ISSN (online)	1698-6946
ISSN	1698-4447
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