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  1. Book ; Online ; E-Book: Toxicoepigenetics

    McCullough, Shaun D. / Dolinoy, Dana C.

    core principles and applications

    2019  

    Author's details edited by Shaun D. McCullough, Dana C. Dolinoy
    Keywords Epigenetics ; Toxicology
    Language English
    Size 1 Online-Ressource (xv, 412 Seiten), Illustrationen, Diagramme
    Publisher Academic Press, an imprint of Elsevier
    Publishing place London
    Publishing country Great Britain
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT019987056
    ISBN 978-0-12-812434-5 ; 0-12-812434-2
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: Liquid application dosing alters the physiology of air-liquid interface (ALI) primary human bronchial epithelial cell/lung fibroblast co-cultures and

    Mallek, Nicholas M / Martin, Elizabeth M / Dailey, Lisa A / McCullough, Shaun D

    Frontiers in toxicology

    2024  Volume 5, Page(s) 1264331

    Abstract: Differentiated primary human bronchial epithelial cell (dpHBEC) cultures grown under air-liquid interface (ALI) conditions exhibit key features of the human respiratory tract and are thus critical for respiratory research as well as efficacy and toxicity ...

    Abstract Differentiated primary human bronchial epithelial cell (dpHBEC) cultures grown under air-liquid interface (ALI) conditions exhibit key features of the human respiratory tract and are thus critical for respiratory research as well as efficacy and toxicity testing of inhaled substances (
    Language English
    Publishing date 2024-01-23
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2673-3080
    ISSN (online) 2673-3080
    DOI 10.3389/ftox.2023.1264331
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Epithelial MAPK signaling directs endothelial NRF2 signaling and IL-8 secretion in a tri-culture model of the alveolar-microvascular interface following diesel exhaust particulate (DEP) exposure.

    Vitucci, Eva C M / Simmons, Alysha E / Martin, Elizabeth M / McCullough, Shaun D

    Particle and fibre toxicology

    2024  Volume 21, Issue 1, Page(s) 15

    Abstract: Background: Particulate matter 2.5 (PM: Methods: An in vitro, tri-culture model of the ACR, incorporating alveolar-like epithelial cells (NCI-H441), pulmonary fibroblasts (IMR90), and pulmonary microvascular endothelial cells (HULEC) was developed to ...

    Abstract Background: Particulate matter 2.5 (PM
    Methods: An in vitro, tri-culture model of the ACR, incorporating alveolar-like epithelial cells (NCI-H441), pulmonary fibroblasts (IMR90), and pulmonary microvascular endothelial cells (HULEC) was developed to investigate cell type-specific molecular responses to a PM
    Results: Alveolar epithelial barrier formation was not perturbed by the 24 h DEP exposure. Despite no alteration in barrier formation, we demonstrate that alveolar epithelial DEP exposure induces transcriptional and protein changes in both the alveolar epithelial cells and the underlying microvascular endothelial cells. Specifically, we show that the underlying microvascular endothelial cells develop redox dysfunction and increase proinflammatory cytokine secretion. Furthermore, we demonstrate that alveolar epithelial MAPK signaling modulates the activation of NRF2 and IL-8 secretion in the underlying microvascular endothelial cells.
    Conclusions: Endothelial redox dysfunction and increased proinflammatory cytokine secretion are two common events in respiratory disease development. These findings highlight new, cell-type specific roles of the alveolar epithelium and microvascular endothelium in the ACR in respiratory disease development following PM
    MeSH term(s) Vehicle Emissions/toxicity ; Endothelial Cells/metabolism ; NF-E2-Related Factor 2/metabolism ; Interleukin-8/metabolism ; Endothelium ; Particulate Matter/toxicity
    Chemical Substances Vehicle Emissions ; NF-E2-Related Factor 2 ; Interleukin-8 ; Particulate Matter
    Language English
    Publishing date 2024-03-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 2170936-1
    ISSN 1743-8977 ; 1743-8977
    ISSN (online) 1743-8977
    ISSN 1743-8977
    DOI 10.1186/s12989-024-00576-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Liquid Application Dosing Alters the Physiology of Air-Liquid Interface Primary Bronchial Epithelial Cultures and In vitro Testing Relevant Endpoints.

    Mallek, Nicholas M / Martin, Elizabeth M / Dailey, Lisa A / McCullough, Shaun D

    Research square

    2023  

    Abstract: Differentiated Primary human bronchial epithelial cell (dpHBEC) cultures grown under air-liquid interface (ALI) conditions exhibit key features of the human respiratory tract and are thus critical for respiratory research as well as efficacy and toxicity ...

    Abstract Differentiated Primary human bronchial epithelial cell (dpHBEC) cultures grown under air-liquid interface (ALI) conditions exhibit key features of the human respiratory tract and are thus critical for respiratory research as well as efficacy and toxicity testing of inhaled substances (
    Language English
    Publishing date 2023-02-24
    Publishing country United States
    Document type Preprint
    DOI 10.21203/rs.3.rs-2570280/v1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Characterizing the extracellular vesicle proteomic landscape of the human airway using

    Vitucci, Eva C M / Carberry, Celeste K / Payton, Alexis / Herring, Laura E / Mordant, Angie L / McCullough, Shaun D / Rager, Julia E

    iScience

    2023  Volume 26, Issue 11, Page(s) 108162

    Abstract: Extracellular vesicle (EV)-mediated intercellular communication significantly influences pulmonary cell health and disease, ... ...

    Abstract Extracellular vesicle (EV)-mediated intercellular communication significantly influences pulmonary cell health and disease, yet
    Language English
    Publishing date 2023-10-10
    Publishing country United States
    Document type Journal Article
    ISSN 2589-0042
    ISSN (online) 2589-0042
    DOI 10.1016/j.isci.2023.108162
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Air Pollution and the Epigenome: A Model Relationship for the Exploration of Toxicoepigenetics.

    McCullough, Shaun D / Dhingra, Radhika / Fortin, Marie C / Diaz-Sanchez, David

    Current opinion in toxicology

    2021  Volume 6, Page(s) 18–25

    Abstract: The field of toxicoepigenetics is rapidly emerging to provide new insights into the relationship between environmental factors, the epigenome, and public health. Toxicoepigenetic data have the potential to revolutionize our understanding of environmental ...

    Abstract The field of toxicoepigenetics is rapidly emerging to provide new insights into the relationship between environmental factors, the epigenome, and public health. Toxicoepigenetic data have the potential to revolutionize our understanding of environmental exposure effects and susceptibility. Studies in recent years have demonstrated that exposure to air pollution alters epigenetic modification states; however, continued advancement of the field is limited by the intrinsic complexity of the epigenome and inherent limitations of different types of studies (epidemiological, clinical, and
    Language English
    Publishing date 2021-03-10
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2468-2934
    ISSN 2468-2934
    DOI 10.1016/j.cotox.2017.07.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Through the Looking Glass:

    Faber, Samantha C / McCullough, Shaun D

    Applied in vitro toxicology

    2019  Volume 4, Issue 2, Page(s) 115–128

    Abstract: With 7 million deaths reported annually from air pollution alone, it is evident that adverse effects of inhaled toxicant exposures remain a major public health concern in the 21st century. Assessment and characterization of the impacts of air pollutants ... ...

    Abstract With 7 million deaths reported annually from air pollution alone, it is evident that adverse effects of inhaled toxicant exposures remain a major public health concern in the 21st century. Assessment and characterization of the impacts of air pollutants on human health stems from epidemiological and clinical studies, which have linked both outdoor and indoor air contaminant exposure to adverse pulmonary and cardiovascular health outcomes. Studies in animal models support epidemiological findings and have been critical in identifying systemic effects of environmental chemicals on cognitive abilities, liver disease, and metabolic dysfunction following inhalation exposure. Likewise, traditional monoculture systems have aided in identifying biomarkers of susceptibility to inhaled toxicants and served as a screening platform for safety assessment of pulmonary toxicants. Despite their contributions,
    Language English
    Publishing date 2019-08-01
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2332-1512
    ISSN 2332-1512
    DOI 10.1089/aivt.2018.0002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Exposure Effects Beyond the Epithelial Barrier: Transepithelial Induction of Oxidative Stress by Diesel Exhaust Particulates in Lung Fibroblasts in an Organotypic Human Airway Model.

    Faber, Samantha C / McNabb, Nicole A / Ariel, Pablo / Aungst, Emily R / McCullough, Shaun D

    Toxicological sciences : an official journal of the Society of Toxicology

    2020  Volume 177, Issue 1, Page(s) 140–155

    Abstract: In vitro bronchial epithelial monoculture models have been pivotal in defining the adverse effects of inhaled toxicant exposures; however, they are only representative of one cellular compartment and may not accurately reflect the effects of exposures on ...

    Abstract In vitro bronchial epithelial monoculture models have been pivotal in defining the adverse effects of inhaled toxicant exposures; however, they are only representative of one cellular compartment and may not accurately reflect the effects of exposures on other cell types. Lung fibroblasts exist immediately beneath the bronchial epithelial barrier and play a central role in lung structure and function, as well as disease development and progression. We tested the hypothesis that in vitro exposure of a human bronchial epithelial cell barrier to the model oxidant diesel exhaust particulates caused transepithelial oxidative stress in the underlying lung fibroblasts using a human bronchial epithelial cell and lung fibroblast coculture model. We observed that diesel exhaust particulates caused transepithelial oxidative stress in underlying lung fibroblasts as indicated by intracellular accumulation of the reactive oxygen species hydrogen peroxide, oxidation of the cellular antioxidant glutathione, activation of NRF2, and induction of oxidative stress-responsive genes. Further, targeted antioxidant treatment of lung fibroblasts partially mitigated the oxidative stress response gene expression in adjacent human bronchial epithelial cells during diesel exhaust particulate exposure. This indicates that exposure-induced oxidative stress in the airway extends beyond the bronchial epithelial barrier and that lung fibroblasts are both a target and a mediator of the adverse effects of inhaled chemical exposures despite being separated from the inhaled material by an epithelial barrier. These findings illustrate the value of coculture models and suggest that transepithelial exposure effects should be considered in inhalation toxicology research and testing.
    MeSH term(s) Bronchi ; Epithelial Cells ; Fibroblasts ; Humans ; Lung ; Oxidative Stress ; Vehicle Emissions/toxicity
    Chemical Substances Vehicle Emissions
    Language English
    Publishing date 2020-06-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1420885-4
    ISSN 1096-0929 ; 1096-6080
    ISSN (online) 1096-0929
    ISSN 1096-6080
    DOI 10.1093/toxsci/kfaa085
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1709: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Linking the Epigenome with Exposure Effects and Susceptibility: The Epigenetic Seed and Soil Model.

    Bowers, Emma C / McCullough, Shaun D

    Toxicological sciences : an official journal of the Society of Toxicology

    2016  Volume 155, Issue 2, Page(s) 302–314

    Abstract: The epigenome is a dynamic mediator of gene expression that shapes the way that cells, tissues, and organisms respond to their environment. Initial studies in the emerging field of "toxicoepigenetics" have described either the impact of an environmental ... ...

    Abstract The epigenome is a dynamic mediator of gene expression that shapes the way that cells, tissues, and organisms respond to their environment. Initial studies in the emerging field of "toxicoepigenetics" have described either the impact of an environmental exposure on the epigenome or the association of epigenetic signatures with the onset or progression of disease; however, the majority of these pioneering studies examined the relationship between discrete epigenetic modifications and the effects of a single environmental factor. Although these data provide critical blocks with which we construct our understanding of the role of the epigenome in susceptibility and disease, they are akin to individual letters in a complex alphabet that is used to compose the language of the epigenome. Advancing the use of epigenetic data to gain a more comprehensive understanding of the mechanisms underlying exposure effects, identify susceptible populations, and inform the next generation risk assessment depends on our ability to integrate these data in a way that accounts for their cumulative impact on gene regulation. Here we will review current examples demonstrating associations between the epigenetic impacts of intrinsic factors, such as such as age, genetics, and sex, and environmental exposures shape the epigenome and susceptibility to exposure effects and disease. We will also demonstrate how the "epigenetic seed and soil" model can be used as a conceptual framework to explain how epigenetic states are shaped by the cumulative impacts of intrinsic and extrinsic factors and how these in turn determine how an individual responds to subsequent exposure to environmental stressors.
    MeSH term(s) Age Factors ; Child, Preschool ; Disease Susceptibility ; Environmental Exposure ; Epigenesis, Genetic ; Female ; Gene-Environment Interaction ; Humans ; Infant ; Male ; Models, Genetic ; Polymorphism, Single Nucleotide ; Pregnancy ; Sex Factors
    Language English
    Publishing date 2016-10-20
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1420885-4
    ISSN 1096-0929 ; 1096-6080
    ISSN (online) 1096-0929
    ISSN 1096-6080
    DOI 10.1093/toxsci/kfw215
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1709: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: Using Chromatin Immunoprecipitation in Toxicology: A Step-by-Step Guide to Increasing Efficiency, Reducing Variability, and Expanding Applications.

    McCullough, Shaun D / On, Doan M / Bowers, Emma C

    Current protocols in toxicology

    2017  Volume 72, Page(s) 3.14.1–3.14.28

    Abstract: Histone modifications work in concert with DNA methylation to regulate cellular structure, function, and response to environmental stimuli. More than 130 unique histone modifications have been described to date, and chromatin immunoprecipitation (ChIP) ... ...

    Abstract Histone modifications work in concert with DNA methylation to regulate cellular structure, function, and response to environmental stimuli. More than 130 unique histone modifications have been described to date, and chromatin immunoprecipitation (ChIP) allows for the exploration of their associations with the regulatory regions of target genes and other DNA/chromatin-associated proteins across the genome. Many variations of ChIP have been developed in the 30 years since its earliest version came into use, which makes it challenging for users to integrate the procedure into their research programs. Furthermore, the differences in ChIP protocols can confound efforts to increase reproducibility across studies. The streamlined ChIP procedure presented here can be readily applied to samples from a wide range of in vitro studies (cell lines and primary cells) and clinical samples (peripheral leukocytes) in toxicology. We also provide detailed guidance on the optimization of critical protocol parameters, such as chromatin fixation, fragmentation, and immunoprecipitation, to increase efficiency and improve reproducibility. Expanding toxicoepigenetic studies to more readily include histone modifications will facilitate a more comprehensive understanding of the role of the epigenome in environmental exposure effects and the integration of epigenetic data in mechanistic toxicology, adverse outcome pathways, and risk assessment. © 2017 by John Wiley & Sons, Inc.
    MeSH term(s) Cell Line ; Chromatin Immunoprecipitation/methods ; DNA/isolation & purification ; Epigenesis, Genetic ; Gene Regulatory Networks ; Gene Targeting ; Histones/metabolism ; Humans ; Leukocytes/chemistry ; Peptide Hydrolases/chemistry ; Polymerase Chain Reaction ; Primary Cell Culture ; Reproducibility of Results ; Sonication ; Toxicology/methods ; Toxicology/standards
    Chemical Substances Histones ; DNA (9007-49-2) ; Peptide Hydrolases (EC 3.4.-)
    Language English
    Publishing date 2017-05-02
    Publishing country United States
    Document type Journal Article
    ISSN 1934-9262
    ISSN (online) 1934-9262
    DOI 10.1002/cptx.22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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