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  1. Article ; Online: Sperm chromatin condensation: epigenetic mechanisms to compact the genome and spatiotemporal regulation from inside and outside the nucleus.

    Okada, Yuki

    Genes & genetic systems

    2022  Volume 97, Issue 1, Page(s) 41–53

    Abstract: Sperm chromatin condensation is a critical step in mammalian spermatogenesis to protect the paternal DNA from external damaging factors and to acquire fertility. During chromatin condensation, various events proceed in a chronological order, ... ...

    Abstract Sperm chromatin condensation is a critical step in mammalian spermatogenesis to protect the paternal DNA from external damaging factors and to acquire fertility. During chromatin condensation, various events proceed in a chronological order, independently or in sequence, interacting with each other both inside and outside the nucleus to support the dramatic chromatin changes. Among these events, histone-protamine replacement, which is concomitant with acrosome biogenesis and cytoskeletal alteration, is the most critical step associated with nuclear elongation. Failures of not only intranuclear events but also extra-nuclear events severely affect sperm shape and chromatin state and are subsequently linked to infertility. This review focuses on nuclear and non-nuclear factors that affect sperm chromatin condensation and its effects, and further discusses the possible utility of sperm chromatin for clinical applications.
    MeSH term(s) Animals ; Chromatin/genetics ; Chromatin Assembly and Disassembly ; Epigenesis, Genetic ; Male ; Mammals/genetics ; Spermatogenesis/genetics ; Spermatozoa
    Chemical Substances Chromatin
    Language English
    Publishing date 2022-04-29
    Publishing country Japan
    Document type Journal Article ; Review
    ZDB-ID 1323536-9
    ISSN 1880-5779 ; 1341-7568
    ISSN (online) 1880-5779
    ISSN 1341-7568
    DOI 10.1266/ggs.21-00065
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3466: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  2. Article ; Online: Sperm chromatin structure: Insights from in vitro to in situ experiments.

    Okada, Yuki

    Current opinion in cell biology

    2022  Volume 75, Page(s) 102075

    Abstract: The sperm genome is tightly packed into a minimal volume of sperm nuclei. Sperm chromatin is highly condensed by protamines (PRMs) after histone-protamine replacement, and the majority of the sperm genome forms a nucleo-protamine structure, namely, the ... ...

    Abstract The sperm genome is tightly packed into a minimal volume of sperm nuclei. Sperm chromatin is highly condensed by protamines (PRMs) after histone-protamine replacement, and the majority of the sperm genome forms a nucleo-protamine structure, namely, the PRM-DNA complex. The outline of sperm chromatin structure was proposed 30 years ago, and the details have been explored by approaches from several independent research fields including male reproduction and infertility, DNA biopolymer, and most recently, genome-wide sequence-based approaches. In this review, the history of research on sperm chromatin structure is briefly described, and the progress of recent related studies is summarized to obtain a more integrated view for the sperm chromatin, an extremely compacted "black box."
    MeSH term(s) Chromatin/genetics ; Chromatin Assembly and Disassembly ; DNA/genetics ; Humans ; Male ; Protamines/chemistry ; Protamines/genetics ; Protamines/metabolism ; Spermatozoa/metabolism
    Chemical Substances Chromatin ; Protamines ; DNA (9007-49-2)
    Language English
    Publishing date 2022-03-25
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 1026381-0
    ISSN 1879-0410 ; 0955-0674
    ISSN (online) 1879-0410
    ISSN 0955-0674
    DOI 10.1016/j.ceb.2022.102075
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2963: Show issues Location:
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  3. Article ; Online: Relapse of Tardive Dyskinesia Following Successful Treatment With Amantadine: A Case Report.

    Kusudo, Keisuke / Uchida, Hiroyuki / Okada, Yuki / Yoshida, Kazunari

    Journal of clinical psychopharmacology

    2024  

    Language English
    Publishing date 2024-03-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604631-9
    ISSN 1533-712X ; 0271-0749
    ISSN (online) 1533-712X
    ISSN 0271-0749
    DOI 10.1097/JCP.0000000000001852
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1664: Show issues Location:
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  4. Article ; Online: Formation of moth-eye-like structures on silicon through

    Nakauchi, Yuki / Minamisawa, Hikari / Okada, Tomohiko

    Dalton transactions (Cambridge, England : 2003)

    2024  Volume 53, Issue 6, Page(s) 2558–2564

    Abstract: Coating subwavelength-scale pinnacles/thorns on surfaces usually results in antireflection, known as "moth-eye effect". However, fabrication of such coatings is often complicated and expensive. Herein, we present a bottom-up approach for forming a moth- ... ...

    Abstract Coating subwavelength-scale pinnacles/thorns on surfaces usually results in antireflection, known as "moth-eye effect". However, fabrication of such coatings is often complicated and expensive. Herein, we present a bottom-up approach for forming a moth-eye-like structure on Si by directly growing layered Mg silicate using a one-step process. When an aqueous solution containing LiF, MgCl
    Language English
    Publishing date 2024-02-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472887-4
    ISSN 1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
    ISSN (online) 1477-9234 ; 1364-5447
    ISSN 0300-9246 ; 1477-9226
    DOI 10.1039/d3dt04105k
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: CDK4/6 inhibitor-induced bone marrow micronuclei might be caused by cell cycle arrest during erythropoiesis.

    Okada, Yuki / Chikura, Satsuki / Kimoto, Takafumi / Iijima, Takeshi

    Genes and environment : the official journal of the Japanese Environmental Mutagen Society

    2024  Volume 46, Issue 1, Page(s) 3

    Abstract: Background: A micronucleus test is generally used to evaluate the genotoxic potential of chemicals. Exaggerated erythropoiesis, as occurs following bleeding, may induce an unexpected increase in micronucleus frequency. This false positive result would ... ...

    Abstract Background: A micronucleus test is generally used to evaluate the genotoxic potential of chemicals. Exaggerated erythropoiesis, as occurs following bleeding, may induce an unexpected increase in micronucleus frequency. This false positive result would be typical in a genotoxicity study due to the enhanced progression of the cell cycle that restores decreased blood cells. The cyclin-dependent kinase (CDK) family is known to play an essential role in preventing genomic instability. Conversely, a selective CDK4/6 inhibitor PD0332991, clinically named Palbociclib, is reported to have genotoxic potential, shown by positive results in both in vitro and in vivo micronucleus studies. To clarify the mechanism by which cell cycle arrest induced by a CDK4/6 inhibitor increases micronucleus frequency, we investigated the positive results of the bone marrow micronucleus test conducted with PD0332991.
    Results: Rats treated with PD0332991 exhibited increased micronucleus frequency in an in vivo bone marrow micronucleus test whereas it was not increased by treatment in human lymphoblastoid TK6 cells. In addition, all other genotoxicity tests including the Ames test and the comet assay showed negative results with PD0332991. Interestingly, PD0332991 treatment led to an increase in erythrocyte size in rats and affected the size distribution of erythrocytes, including the micronucleus. The mean corpuscular volume of reticulocytes (MCVr) in the PD0332991 treatment group was significantly increased compared to that of the vehicle control (83.8 fL in the PD0332991, and 71.6 fL in the vehicle control.). Further, the average micronucleated erythrocytes (MNE) size of the PD0332991 group and vehicle control was 8.2 and 7.3 µm, respectively. In the histogram, the vehicle control showed a monomodal distribution with a peak near 7.3 µm. In contrast, the PD0332991 group showed a bimodal distribution with peaks around 7.5 and 8.5 µm. Micronucleated erythrocytes in the PD0332991 group were significantly larger than those in the vehicle control. These results suggest that the increase in micronucleus frequency induced by the CDK4/6 inhibitor is not due to genotoxicity, but is attributable to disturbance of the cell cycle, differentiation, and enucleation of erythroblasts.
    Conclusions: It was suggested that the positive outcome of the in vivo bone marrow micronucleus test resulting from treatment with PD0332991 could not be attributed to its genotoxicity. Further studies to clarify the mechanism of action can contribute to the development of drug candidate compounds lacking intrinsic genotoxic effects.
    Language English
    Publishing date 2024-02-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2269162-5
    ISSN 1880-7062 ; 1880-7046
    ISSN (online) 1880-7062
    ISSN 1880-7046
    DOI 10.1186/s41021-024-00298-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Development of a Sensitive Anti-Mouse CD39 Monoclonal Antibody (C

    Okada, Yuki / Suzuki, Hiroyuki / Kaneko, Mika K / Kato, Yukinari

    Monoclonal antibodies in immunodiagnosis and immunotherapy

    2024  Volume 43, Issue 1, Page(s) 24–31

    Abstract: CD39 is involved in adenosine metabolism by converting extracellular ATP to adenosine. As extracellular adenosine plays a critical role in the immune suppression of the tumor microenvironment, the inhibition of CD39 activity by monoclonal antibodies ( ... ...

    Abstract CD39 is involved in adenosine metabolism by converting extracellular ATP to adenosine. As extracellular adenosine plays a critical role in the immune suppression of the tumor microenvironment, the inhibition of CD39 activity by monoclonal antibodies (mAbs) is one of the important strategies for tumor therapy. This study developed specific and sensitive mAbs for mouse CD39 (mCD39) using the Cell-Based Immunization and Screening method. The established anti-mCD39 mAb, C
    MeSH term(s) Cricetinae ; Mice ; Rats ; Animals ; Antibodies, Monoclonal ; Cricetulus ; Flow Cytometry ; CHO Cells ; Kinetics ; Blotting, Western ; Adenosine
    Chemical Substances Antibodies, Monoclonal ; Adenosine (K72T3FS567)
    Language English
    Publishing date 2024-01-10
    Publishing country United States
    Document type Journal Article
    ISSN 2167-9436
    ISSN (online) 2167-9436
    DOI 10.1089/mab.2023.0016
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Boy with abdominal distension.

    Okada, Yuki / Iio, Kazuki

    Journal of paediatrics and child health

    2021  Volume 58, Issue 6, Page(s) 1113

    MeSH term(s) Constipation ; Digestive System Abnormalities ; Humans ; Male
    Language English
    Publishing date 2021-07-06
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 1024476-1
    ISSN 1440-1754 ; 1034-4810
    ISSN (online) 1440-1754
    ISSN 1034-4810
    DOI 10.1111/jpc.15632
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 227: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  8. Article ; Online: Methicillin-resistant Staphylococcus aureus Bacteremia With Splenic Abscess Masked by Epstein-Barr Virus-associated Hemophagocytic Lymphohistiocytosis.

    Banno, Yoshinori / Okada, Hiroshi / Naruse, Yuki / Hiramoto, Ryugo

    The Pediatric infectious disease journal

    2023  Volume 42, Issue 4, Page(s) e136–e137

    MeSH term(s) Humans ; Lymphohistiocytosis, Hemophagocytic/complications ; Lymphohistiocytosis, Hemophagocytic/diagnosis ; Herpesvirus 4, Human ; Epstein-Barr Virus Infections/complications ; Epstein-Barr Virus Infections/diagnosis ; Methicillin-Resistant Staphylococcus aureus ; Splenic Diseases/diagnosis ; Abscess ; Bacteremia/complications ; Bacteremia/diagnosis ; Intraabdominal Infections
    Language English
    Publishing date 2023-01-23
    Publishing country United States
    Document type Letter
    ZDB-ID 392481-6
    ISSN 1532-0987 ; 0891-3668
    ISSN (online) 1532-0987
    ISSN 0891-3668
    DOI 10.1097/INF.0000000000003837
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1852: Show issues Location:
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  9. Article: Analyses of mRNA Expression Levels of Pituitary Hormones, Their Hypothalamic Regulating Factors, and Receptors Involved in Metamorphosis with Special Reference to the Summer and Winter Seasons.

    Matsumoto, Yuki / Kowata, Natsuno / Kikuyama, Sakae / Okada, Reiko

    Zoological science

    2023  Volume 41, Issue 1, Page(s) 32–38

    Abstract: Bullfrog ( ...

    Abstract Bullfrog (
    MeSH term(s) Animals ; Seasons ; Pituitary Hormones ; Prolactin ; Japan ; Larva/genetics
    Chemical Substances Pituitary Hormones ; Prolactin (9002-62-4)
    Language English
    Publishing date 2023-04-12
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2038883-4
    ISSN 2212-3830 ; 0289-0003
    ISSN (online) 2212-3830
    ISSN 0289-0003
    DOI 10.2108/zs230080
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  10. Article ; Online: CUT&Tag Using "Stress-Free" Con A-Conjugated Dynabeads

    Fujiwara, Yasuhiro / Okada, Yuki

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2519, Page(s) 141–153

    Abstract: Epigenome research has employed various methods to identify the genomic location of proteins of interest, such as transcription factors and histone modifications. CUT&Tag is a recently established method used in epigenome research to determine the ... ...

    Abstract Epigenome research has employed various methods to identify the genomic location of proteins of interest, such as transcription factors and histone modifications. CUT&Tag is a recently established method used in epigenome research to determine the genomic location of proteins of interest, such as transcription factors and histone modifications. In CUT&Tag method, cells are bound and hold on concanavalin A (con A)-coated magnetic beads, then a Protein-A Tn5 transposase fusion protein cuts the genome and inserts adapter sequences nearby the target protein. Here we describe the updated CUT&Tag procedure using "home-made" con A-conjugated magnetic beads. This method is free of poor suspendability and severe aggregation, hence providing improved sensitivity.
    MeSH term(s) Concanavalin A ; Genome ; Histone Code ; Protein Processing, Post-Translational ; Transcription Factors/metabolism
    Chemical Substances Transcription Factors ; Concanavalin A (11028-71-0)
    Language English
    Publishing date 2022-09-06
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2433-3_16
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