LIVIVO - Search results -

Search results

Result 1 - 10 of total 26

Search options

Article: Marsupial milk: a fluid source of nutrition and immune factors for the developing pouch young.

Pharo, Elizabeth A

Reproduction, fertility, and development

2019 Volume 31, Issue 7, Page(s) 1252–1265

Abstract: Marsupials have a very different reproductive strategy to eutherians. An Australian marsupial, the tammar wallaby (Macropus eugenii) has a very short pregnancy of about 26.5 days, with a comparatively long lactation of 300-350 days. The tammar mother ... ...

Abstract	Marsupials have a very different reproductive strategy to eutherians. An Australian marsupial, the tammar wallaby (Macropus eugenii) has a very short pregnancy of about 26.5 days, with a comparatively long lactation of 300-350 days. The tammar mother gives birth to an altricial, approximately 400 mg young that spends the first 200 days postpartum (p.p.) in its mother's pouch, permanently (0-100 days p.p.; Phase 2A) and then intermittently (100-200 days p.p.; Phase 2B) attached to the teat. The beginning of Phase 3 marks the first exit from the pouch (akin to the birth of a precocious eutherian neonate) and the supplementation of milk with herbage. The marsupial mother progressively alters milk composition (proteins, fats and carbohydrates) and individual milk constituents throughout the lactation cycle to provide nutrients and immunological factors that are appropriate for the considerable physiological development and growth of her pouch young. This review explores the changes in tammar milk components that occur during the lactation cycle in conjunction with the development of the young.
MeSH term(s)	Animals ; Animals, Newborn/growth & development ; Female ; Lactation/physiology ; Marsupialia/physiology ; Milk ; Nutritional Status
Language	English
Publishing date	2019-01-09
Publishing country	Australia
Document type	Journal Article ; Review
ZDB-ID	1019913-5
ISSN	1448-5990 ; 1031-3613
ISSN (online)	1448-5990
ISSN	1031-3613
DOI	10.1071/RD18197
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2756: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Marsupial milk: a fluid source of nutrition and immune factors for the developing pouch young

Pharo, Elizabeth A

Reproduction, fertility, and development. 2019, v. 31, no. 7

2019

Abstract	Marsupials have a very different reproductive strategy to eutherians. An Australian marsupial, the tammar wallaby (Macropus eugenii) has a very short pregnancy of about 26.5 days, with a comparatively long lactation of 300–350 days. The tammar mother gives birth to an altricial, approximately 400 mg young that spends the first 200 days postpartum (p.p.) in its mother’s pouch, permanently (0–100 days p.p.; Phase 2A) and then intermittently (100–200 days p.p.; Phase 2B) attached to the teat. The beginning of Phase 3 marks the first exit from the pouch (akin to the birth of a precocious eutherian neonate) and the supplementation of milk with herbage. The marsupial mother progressively alters milk composition (proteins, fats and carbohydrates) and individual milk constituents throughout the lactation cycle to provide nutrients and immunological factors that are appropriate for the considerable physiological development and growth of her pouch young. This review explores the changes in tammar milk components that occur during the lactation cycle in conjunction with the development of the young.
Keywords	carbohydrates ; forage ; immunologic factors ; lactation ; lipids ; Macropus eugenii ; milk ; milk composition ; neonates ; nutrients ; nutrition ; pregnancy ; proteins
Language	English
Size	p. 1252-1265.
Publishing place	CSIRO Publishing
Document type	Article
ZDB-ID	1019913-5
ISSN	1448-5990 ; 1031-3613
ISSN (online)	1448-5990
ISSN	1031-3613
DOI	10.1071/RD18197
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 5258: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Expression of the mammary gland-specific tammar wallaby early lactation protein gene is maintained in vitro in the absence of prolactin.

Pharo, Elizabeth A

Molecular and cellular endocrinology

2014 Volume 382, Issue 2, Page(s) 871–880

Abstract: Marsupial ELP (early lactation protein) and its eutherian orthologue, CTI (colostrum trypsin inhibitor) are expressed in the mammary gland only for the first 100 days postpartum (Phase 2A) in the tammar wallaby and during the bovine and canine ... ...

Abstract	Marsupial ELP (early lactation protein) and its eutherian orthologue, CTI (colostrum trypsin inhibitor) are expressed in the mammary gland only for the first 100 days postpartum (Phase 2A) in the tammar wallaby and during the bovine and canine colostrogenesis period 24-36h postpartum respectively. The factors which regulate temporal ELP and CTI expression are unknown. A tammar mammary gland explant culture model was used to investigate ELP gene regulation during pregnancy and early- and mid-lactation (Phase 1, 2A and 2B respectively). Tammar ELP expression could only be manipulated in explants in vitro if the gene was already expressed in vivo. ELP expression was maximal in Phase 1 explants treated with lactogenic hormones (insulin, hydrocortisone and prolactin), but unlike LGB (β-lactoglobulin), ELP expression was maintained in insulin or insulin and hydrocortisone over a 12-day culture period. In contrast, ELP was down-regulated when cultured without hormones. ELP could not be induced in explants cultured from mid-lactation which suggested that transcriptional repressors may prevent ELP expression during this period.
MeSH term(s)	Animals ; Colostrum/chemistry ; Female ; Gene Expression Regulation ; Hydrocortisone/pharmacology ; Insulin/pharmacology ; Lactation/genetics ; Lactoglobulins/genetics ; Lactoglobulins/metabolism ; Macropodidae ; Mammary Glands, Animal/drug effects ; Mammary Glands, Animal/metabolism ; Milk Proteins/genetics ; Milk Proteins/metabolism ; Pregnancy ; Prolactin/pharmacology ; Time Factors ; Tissue Culture Techniques ; Transcription, Genetic
Chemical Substances	Insulin ; Lactoglobulins ; Milk Proteins ; Prolactin (9002-62-4) ; Hydrocortisone (WI4X0X7BPJ)
Language	English
Publishing date	2014-02-15
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	187438-x
ISSN	1872-8057 ; 0303-7207
ISSN (online)	1872-8057
ISSN	0303-7207
DOI	10.1016/j.mce.2013.10.030
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1127: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: A Modified Bicanalicular Crawford Placement Method for Congenital Nasolacrimal Duct Obstruction: Reducing Need for Operative Room Removal.

Thompson, John P / Pharo, Austin M / Ingram, Ellen E / Bozner, Elizabeth M / Avdic, Armin / Ellis, George S

Ophthalmic plastic and reconstructive surgery

2023 Volume 39, Issue 6, Page(s) 628–631

Abstract: Purpose: This study introduces a method for Crawford bicanalicular stent placement for congenital nasolacrimal duct obstruction by looping the ends to themselves which are tied together with dissolvable sutures to ease in-office removal.: Methods: ... ...

Abstract	Purpose: This study introduces a method for Crawford bicanalicular stent placement for congenital nasolacrimal duct obstruction by looping the ends to themselves which are tied together with dissolvable sutures to ease in-office removal. Methods: This is a single institution, retrospective study that evaluates outcomes of patients aged 5 years and under who underwent bicanalicular stenting for congenital nasolacrimal duct obstruction by a single surgeon (G.S.E.) between 2004 and 2020. Only primary surgeries were included in the analysis. Stenting could be accompanied by balloon dilatation and/or turbinate infracture. Age, sex, follow-up time, complications, type of intervention, extrusion, recurrence, and operative room removal were recorded. Results: This study included 56 eyes from 54 patients with a mean age of 19.0 ± 9.5 months (range, 8-50 months). There was a 30.3% extrusion rate, a 5.4% rate of recurrence of disease, and a 3.6% rate of operative room removal. The average follow-up time was 25.1 ± 39.8 months (range, 1-132 months). For patients with or without extrusion, there were no significant differences between age, sex, laterality, type of intervention, follow-up time, or rate of recurrence. Each eye that had recurrence (3 total) or needed operative room removal (2 total) underwent only bicanalicular stenting without accompanying procedures, although the difference in rates between procedures was also not statistically significant. Conclusions: This method had a low recurrence and operative room removal rate, with similar extrusion and complication rates to other bicanalicular stent and intubation methods for the treatment of congenital nasolacrimal duct obstruction.
MeSH term(s)	Humans ; Infant ; Child, Preschool ; Lacrimal Duct Obstruction/etiology ; Nasolacrimal Duct/surgery ; Nasolacrimal Duct/abnormalities ; Retrospective Studies ; Dacryocystorhinostomy/methods ; Intubation/methods ; Eye Abnormalities/etiology ; Treatment Outcome
Language	English
Publishing date	2023-07-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	632830-1
ISSN	1537-2677 ; 0740-9303
ISSN (online)	1537-2677
ISSN	0740-9303
DOI	10.1097/IOP.0000000000002460
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2159: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Expression of the mammary gland-specific tammar wallaby early lactation protein gene is maintained in vitro in the absence of prolactin

Pharo, Elizabeth A

Molecular and Cellular Endocrinology. 2014 Feb. 15, v. 382

2014

Abstract: Marsupial ELP (early lactation protein) and its eutherian orthologue, CTI (colostrum trypsin inhibitor) are expressed in the mammary gland only for the first 100days postpartum (Phase 2A) in the tammar wallaby and during the bovine and canine ... ...

Abstract	Marsupial ELP (early lactation protein) and its eutherian orthologue, CTI (colostrum trypsin inhibitor) are expressed in the mammary gland only for the first 100days postpartum (Phase 2A) in the tammar wallaby and during the bovine and canine colostrogenesis period 24â36h postpartum respectively. The factors which regulate temporal ELP and CTI expression are unknown. A tammar mammary gland explant culture model was used to investigate ELP gene regulation during pregnancy and early- and mid-lactation (Phase 1, 2A and 2B respectively). Tammar ELP expression could only be manipulated in explants in vitro if the gene was already expressed in vivo. ELP expression was maximal in Phase 1 explants treated with lactogenic hormones (insulin, hydrocortisone and prolactin), but unlike LGB (Î²-lactoglobulin), ELP expression was maintained in insulin or insulin and hydrocortisone over a 12-day culture period. In contrast, ELP was down-regulated when cultured without hormones. ELP could not be induced in explants cultured from mid-lactation which suggested that transcriptional repressors may prevent ELP expression during this period.
Keywords	beta-lactoglobulin ; cattle ; colostrum ; cortisol ; dogs ; early lactation ; explants ; genes ; insulin ; Macropus eugenii ; mammary glands ; models ; pregnancy ; prolactin ; repressor proteins ; trypsin inhibitors
Language	English
Dates of publication	2014-0215
Size	p. 871-880.
Publishing place	Elsevier Ireland Ltd
Document type	Article
ZDB-ID	187438-x
ISSN	1872-8057 ; 0303-7207
ISSN (online)	1872-8057
ISSN	0303-7207
DOI	10.1016/j.mce.2013.10.030
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 1127: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Host-Pathogen Responses to Pandemic Influenza H1N1pdm09 in a Human Respiratory Airway Model.

Pharo, Elizabeth A / Williams, Sinéad M / Boyd, Victoria / Sundaramoorthy, Vinod / Durr, Peter A / Baker, Michelle L

Viruses

2020 Volume 12, Issue 6

Abstract: The respiratory Influenza A Viruses (IAVs) and emerging zoonotic viruses such as Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) pose a significant threat to human health. To accelerate our understanding of the host-pathogen response to ... ...

Abstract	The respiratory Influenza A Viruses (IAVs) and emerging zoonotic viruses such as Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) pose a significant threat to human health. To accelerate our understanding of the host-pathogen response to respiratory viruses, the use of more complex in vitro systems such as normal human bronchial epithelial (NHBE) cell culture models has gained prominence as an alternative to animal models. NHBE cells were differentiated under air-liquid interface (ALI) conditions to form an in vitro pseudostratified epithelium. The responses of well-differentiated (wd) NHBE cells were examined following infection with the 2009 pandemic Influenza A/H1N1pdm09 strain or following challenge with the dsRNA mimic, poly(I:C). At 30 h postinfection with H1N1pdm09, the integrity of the airway epithelium was severely impaired and apical junction complex damage was exhibited by the disassembly of zona occludens-1 (ZO-1) from the cell cytoskeleton. wdNHBE cells produced an innate immune response to IAV-infection with increased transcription of pro- and anti-inflammatory cytokines and chemokines and the antiviral
MeSH term(s)	Animals ; Bronchi/cytology ; Bronchi/virology ; Cells, Cultured ; Chemokines/metabolism ; Cytokines/metabolism ; Dogs ; Host-Pathogen Interactions ; Humans ; Immunity, Innate ; Influenza A Virus, H1N1 Subtype/immunology ; Influenza A Virus, H1N1 Subtype/physiology ; Influenza, Human/epidemiology ; Influenza, Human/virology ; Intercellular Junctions ; Madin Darby Canine Kidney Cells ; Models, Biological ; Mucin 5AC/metabolism ; Pandemics ; Respiratory Mucosa/cytology ; Respiratory Mucosa/virology ; Virus Cultivation
Chemical Substances	Chemokines ; Cytokines ; MUC5AC protein, human ; Mucin 5AC
Keywords	covid19
Language	English
Publishing date	2020-06-24
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v12060679
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Host–Pathogen Responses to Pandemic Influenza H1N1pdm09 in a Human Respiratory Airway Model

Pharo, Elizabeth A / Williams, Sinéad M / Boyd, Victoria / Sundaramoorthy, Vinod / Durr, Peter A / Baker, Michelle L

Viruses. 2020 June 24, v. 12, no. 6

2020

Abstract	The respiratory Influenza A Viruses (IAVs) and emerging zoonotic viruses such as Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) pose a significant threat to human health. To accelerate our understanding of the host–pathogen response to respiratory viruses, the use of more complex in vitro systems such as normal human bronchial epithelial (NHBE) cell culture models has gained prominence as an alternative to animal models. NHBE cells were differentiated under air-liquid interface (ALI) conditions to form an in vitro pseudostratified epithelium. The responses of well-differentiated (wd) NHBE cells were examined following infection with the 2009 pandemic Influenza A/H1N1pdm09 strain or following challenge with the dsRNA mimic, poly(I:C). At 30 h postinfection with H1N1pdm09, the integrity of the airway epithelium was severely impaired and apical junction complex damage was exhibited by the disassembly of zona occludens-1 (ZO-1) from the cell cytoskeleton. wdNHBE cells produced an innate immune response to IAV-infection with increased transcription of pro- and anti-inflammatory cytokines and chemokines and the antiviral viperin but reduced expression of the mucin-encoding MUC5B, which may impair mucociliary clearance. Poly(I:C) produced similar responses to IAV, with the exception of MUC5B expression which was more than 3-fold higher than for control cells. This study demonstrates that wdNHBE cells are an appropriate ex-vivo model system to investigate the pathogenesis of respiratory viruses.
Keywords	Influenza A virus ; Severe acute respiratory syndrome coronavirus 2 ; animal models ; cell culture ; chemokines ; cytoskeleton ; double-stranded RNA ; epithelium ; human health ; humans ; influenza ; innate immunity ; liquid-air interface ; pandemic ; pathogenesis ; viruses
Language	English
Dates of publication	2020-0624
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v12060679
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Mammary cell-activating factor regulates the hormone-independent transcription of the early lactation protein (ELP) gene in a marsupial.

Pharo, Elizabeth A / Renfree, Marilyn B / Cane, Kylie N

Molecular and cellular endocrinology

2016 Volume 436, Page(s) 169–182

Abstract: The regulation of the tammar wallaby (Macropus eugenii) early lactation protein (ELP) gene is complex. ELP is responsive to the lactogenic hormones; insulin (I), hydrocortisone (HC) and prolactin (PRL) in mammary gland explants but could not be induced ... ...

Abstract	The regulation of the tammar wallaby (Macropus eugenii) early lactation protein (ELP) gene is complex. ELP is responsive to the lactogenic hormones; insulin (I), hydrocortisone (HC) and prolactin (PRL) in mammary gland explants but could not be induced with lactogenic hormones in tammar primary mammary gland cells, nor in KIM-2 conditionally immortalised murine mammary epithelial cells. Similarly, ELP promoter constructs transiently-transfected into human embryonic kidney (HEK293T) cells constitutively expressing the prolactin receptor (PRLR) and Signal Transducer and Activator of Transcription (STAT)5A were unresponsive to prolactin, unlike the rat and mouse β-casein (CSN2) promoter constructs. Identification of the minimal promoter required for the hormone-independent transcription of tammar ELP in HEK293Ts and comparative analysis of the proximal promoters of marsupial ELP and the orthologous eutherian colostrum trypsin inhibitor (CTI) gene suggests that mammary cell-activating factor (MAF), an E26 transformation-specific (ETS) factor, may bind to an AGGAAG motif and activate tammar ELP.
MeSH term(s)	Animals ; Base Sequence ; Binding Sites/genetics ; Cells, Cultured ; Conserved Sequence/genetics ; Female ; Hormones/pharmacology ; Macropodidae/genetics ; Mammary Glands, Animal/metabolism ; Mice ; Milk Proteins/genetics ; Milk Proteins/metabolism ; Promoter Regions, Genetic/genetics ; Proteins/genetics ; Proteins/metabolism ; Rats ; Response Elements/genetics ; Sequence Alignment ; Transcription Factors/metabolism ; Transcription Initiation Site/drug effects ; Transcription, Genetic/drug effects
Chemical Substances	Hormones ; Milk Proteins ; Proteins ; Transcription Factors
Language	English
Publishing date	2016-07-21
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	187438-x
ISSN	1872-8057 ; 0303-7207
ISSN (online)	1872-8057
ISSN	0303-7207
DOI	10.1016/j.mce.2016.07.023
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1127: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: ACE2 Expression in Organotypic Human Airway Epithelial Cultures and Airway Biopsies.

Chen, Qianyu / Langenbach, Shenna / Li, Meina / Xia, Yuxiu C / Gao, Xumei / Gartner, Matthew J / Pharo, Elizabeth A / Williams, Sinéad M / Todd, Shawn / Clarke, Nadeene / Ranganathan, Sarath / Baker, Michelle L / Subbarao, Kanta / Stewart, Alastair G

Frontiers in pharmacology

2022 Volume 13, Page(s) 813087

Abstract: Coronavirus disease 2019 (COVID-19) caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an acute respiratory disease with systemic complications. Therapeutic strategies for COVID-19, including repurposing ( ... ...

Abstract	Coronavirus disease 2019 (COVID-19) caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an acute respiratory disease with systemic complications. Therapeutic strategies for COVID-19, including repurposing (partially) developed drugs are urgently needed, regardless of the increasingly successful vaccination outcomes. We characterized two-dimensional (2D) and three-dimensional models (3D) to establish a physiologically relevant airway epithelial model with potential for investigating SARS-CoV-2 therapeutics. Human airway basal epithelial cells maintained in submerged 2D culture were used at low passage to retain the capacity to differentiate into ciliated, club, and goblet cells in both air-liquid interface culture (ALI) and airway organoid cultures, which were then analyzed for cell phenotype makers. Airway biopsies from non-asthmatic and asthmatic donors enabled comparative evaluation of the level and distribution of immunoreactive angiotensin-converting enzyme 2 (ACE2). ACE2 and transmembrane serine proteinase 2 (TMPRSS2) mRNA were expressed in ALI and airway organoids at levels similar to those of native (i.e., non-cultured) human bronchial epithelial cells, whereas furin expression was more faithfully represented in ALI. ACE2 was mainly localized to ciliated and basal epithelial cells in human airway biopsies, ALI, and airway organoids. Cystic fibrosis appeared to have no influence on ACE2 gene expression. Neither asthma nor smoking status had consistent marked influence on the expression or distribution of ACE2 in airway biopsies. SARS-CoV-2 infection of ALI cultures did not increase the levels of selected cytokines. Organotypic, and particularly ALI airway cultures are useful and practical tools for investigation of SARS-CoV-2 infection and evaluating the clinical potential of therapeutics for COVID-19.
Language	English
Publishing date	2022-03-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.813087
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Host-Pathogen Responses to Pandemic Influenza H1N1pdm09 in a Human Respiratory Airway Model

Pharo, Elizabeth A / Williams, Sinéad M / Boyd, Victoria / Sundaramoorthy, Vinod / Durr, Peter A / Baker, Michelle L

Viruses

Abstract	The respiratory Influenza A Viruses (IAVs) and emerging zoonotic viruses such as Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) pose a significant threat to human health. To accelerate our understanding of the host-pathogen response to respiratory viruses, the use of more complex in vitro systems such as normal human bronchial epithelial (NHBE) cell culture models has gained prominence as an alternative to animal models. NHBE cells were differentiated under air-liquid interface (ALI) conditions to form an in vitro pseudostratified epithelium. The responses of well-differentiated (wd) NHBE cells were examined following infection with the 2009 pandemic Influenza A/H1N1pdm09 strain or following challenge with the dsRNA mimic, poly(I:C). At 30 h postinfection with H1N1pdm09, the integrity of the airway epithelium was severely impaired and apical junction complex damage was exhibited by the disassembly of zona occludens-1 (ZO-1) from the cell cytoskeleton. wdNHBE cells produced an innate immune response to IAV-infection with increased transcription of pro- and anti-inflammatory cytokines and chemokines and the antiviral viperin but reduced expression of the mucin-encoding MUC5B, which may impair mucociliary clearance. Poly(I:C) produced similar responses to IAV, with the exception of MUC5B expression which was more than 3-fold higher than for control cells. This study demonstrates that wdNHBE cells are an appropriate ex-vivo model system to investigate the pathogenesis of respiratory viruses.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #620517
Database	COVID19

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Kategorien

Inter-library loan at ZB MED