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Article: Optical Properties of Conical Quantum Dot: Exciton-Related Raman Scattering, Interband Absorption and Photoluminescence.

Gavalajyan, Sargis P / Mantashian, Grigor A / Kharatyan, Gor Ts / Sarkisyan, Hayk A / Mantashyan, Paytsar A / Baskoutas, Sotirios / Hayrapetyan, David B

Nanomaterials (Basel, Switzerland)

2023 Volume 13, Issue 8

Abstract: The current work used the effective mass approximation conjoined with the finite element method to study the exciton states in a conical GaAs quantum dot. In particular, the dependence of the exciton energy on the geometrical parameters of a conical ... ...

Abstract	The current work used the effective mass approximation conjoined with the finite element method to study the exciton states in a conical GaAs quantum dot. In particular, the dependence of the exciton energy on the geometrical parameters of a conical quantum dot has been studied. Once the one-particle eigenvalue equations have been solved, both for electrons and holes, the available information on energies and wave functions is used as input to calculate exciton energy and the effective band gap of the system. The lifetime of an exciton in a conical quantum dot has been estimated and shown to be in the range of nanoseconds. In addition, exciton-related Raman scattering, interband light absorption and photoluminescence in conical GaAs quantum dots have been calculated. It has been shown that with a decrease in the size of the quantum dot, the absorption peak has a blue shift, which is more pronounced for quantum dots of smaller sizes. Furthermore, the interband optical absorption and photoluminescence spectra have been revealed for different sizes of GaAs quantum dot.
Language	English
Publishing date	2023-04-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano13081393
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Article ; Online: The 5-HT7 receptor is involved in allocentric spatial memory information processing.

Sarkisyan, Gor / Hedlund, Peter B

Behavioural brain research

2009 Volume 202, Issue 1, Page(s) 26–31

Abstract: The hippocampus has been implicated in aspects of spatial memory. Its ability to generate new neurons has been suggested to play a role in memory formation. Hippocampal serotonin (5-HT) neurotransmission has also been proposed as a contributor to memory ... ...

Abstract	The hippocampus has been implicated in aspects of spatial memory. Its ability to generate new neurons has been suggested to play a role in memory formation. Hippocampal serotonin (5-HT) neurotransmission has also been proposed as a contributor to memory processing. Studies have shown that the 5-HT(7) receptor is present in the hippocampus in relatively high abundance. Thus the aim of the present study was to investigate the possible role of the 5-HT(7) receptor in spatial memory using 5-HT(7) receptor-deficient mice (5-HT(7)(-/-)). A hippocampus-associated spatial memory deficit in 5-HT(7)(-/-) mice was demonstrated using a novel location/novel object test. A similar reduction in novel location exploration was observed in C57BL/6J mice treated with the selective 5-HT(7) receptor antagonist SB-269970. These findings prompted an extended analysis using the Barnes maze demonstrating that 5-HT(7)(-/-) mice were less efficient in accommodating to changes in spatial arrangement than 5-HT(7)(+/+) mice. 5-HT(7)(-/-) mice had specific impairments in memory compilation required for resolving spatial tasks, which resulted in impaired allocentric spatial memory whereas egocentric spatial memory remained intact after the mice were forced to switch back from striatum-dependent egocentric to hippocampus-dependent allocentric memory. To further investigate the physiological bases underlining these behaviors we compared hippocampal neurogenesis in 5-HT(7)(+/+) and 5-HT(7)(-/-) mice employing BrdU immunohistochemistry. The rate of cell proliferation in the dentate gyrus was identical in the two genotypes. From the current data we conclude that the 5-HT(7)(-/-) mice performed by remembering a simple sequence of actions that resulted in successfully locating a hidden target in a static environment.
MeSH term(s)	Animals ; Bromodeoxyuridine ; Cell Count ; Cell Proliferation ; Dentate Gyrus/drug effects ; Dentate Gyrus/physiology ; Exploratory Behavior/drug effects ; Exploratory Behavior/physiology ; Immunohistochemistry ; Male ; Maze Learning/drug effects ; Maze Learning/physiology ; Memory/drug effects ; Memory/physiology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Neurogenesis/physiology ; Phenols/administration & dosage ; Receptors, Serotonin/genetics ; Receptors, Serotonin/metabolism ; Recognition, Psychology/drug effects ; Recognition, Psychology/physiology ; Serotonin Antagonists/administration & dosage ; Space Perception/drug effects ; Space Perception/physiology ; Spatial Behavior/drug effects ; Spatial Behavior/physiology ; Sulfonamides/administration & dosage
Chemical Substances	Phenols ; Receptors, Serotonin ; SB 269970 ; Serotonin Antagonists ; Sulfonamides ; serotonin 7 receptor ; Bromodeoxyuridine (G34N38R2N1)
Language	English
Publishing date	2009-03-19
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	449927-x
ISSN	1872-7549 ; 0166-4328
ISSN (online)	1872-7549
ISSN	0166-4328
DOI	10.1016/j.bbr.2009.03.011
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Article ; Online: The 5-HT(7) receptor as a mediator and modulator of antidepressant-like behavior.

Sarkisyan, Gor / Roberts, Amanda J / Hedlund, Peter B

Behavioural brain research

2010 Volume 209, Issue 1, Page(s) 99–108

Abstract: The 5-HT(7) receptor has been suggested as a target for treating depression since inactivation or blockade of the receptor has an antidepressant-like behavioral effect. The present study investigated possible interactions between various classes of drugs ...

Abstract	The 5-HT(7) receptor has been suggested as a target for treating depression since inactivation or blockade of the receptor has an antidepressant-like behavioral effect. The present study investigated possible interactions between various classes of drugs with antidepressant properties and blockade or inactivation of the 5-HT(7) receptor. Immobility despair in the tail suspension test and the forced swim test was evaluated in mice lacking the 5-HT(7) receptor (5-HT(7)(-/-)) and in wild-type controls (5-HT(7)(+/+)) following acute drug treatments. Citalopram, a selective serotonin reuptake inhibitor and widely used antidepressant, dose-dependently reduced immobility in the tail suspension test in both 5-HT(7)(+/+) and 5-HT(7)(-/-) mice. Combining doses of citalopram and the 5-HT(7) receptor antagonist SB-269970 that by themselves did not affect behavior, reduced immobility in 5-HT(7)(+/+) mice in both the tail suspension test and the forced swim test. No effect was seen in 5-HT(7)(-/-) mice. Desipramine and reboxetine, two norepinephrine reuptake inhibitors, dose-dependently reduced immobility in the tail suspension test in 5-HT(7)(+/+) mice, but had no effect in 5-HT(7)(-/-) mice. A synergistic effect between desipramine and SB-269970 was found in both behavioral tests in 5-HT(7)(+/+) mice. Reboxetine combined with SB-269970 had effect only in the forced swim test. GBR 12909, a dopamine reuptake inhibitor, dose-dependently reduced tail suspension test immobility in both genotypes. There was no interaction between GBR 12909 and SB-269970. Aripiprazole, an antipsychotic, reduced immobility in both tests in 5-HT(7)(+/+) mice, but not in 5-HT(7)(-/-) mice. The results show that the 5-HT(7) receptor is required for the observed interaction between this receptor and antidepressants such as citalopram. The data furthermore support the hypothesis that the 5-HT(7) receptor might be a suitable target for treating depression.
MeSH term(s)	Animals ; Antidepressive Agents/pharmacology ; Antipsychotic Agents/pharmacology ; Aripiprazole ; Citalopram/pharmacology ; Corticosterone/blood ; Depression/genetics ; Disease Models, Animal ; Dopamine Uptake Inhibitors/pharmacology ; Dose-Response Relationship, Drug ; Drug Synergism ; Hindlimb Suspension/methods ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Motor Activity/drug effects ; Phenols/pharmacology ; Piperazines/pharmacology ; Quinolones/pharmacology ; Receptors, Serotonin/deficiency ; Receptors, Serotonin/metabolism ; Serotonin Antagonists/pharmacology ; Sulfonamides/pharmacology ; Swimming/psychology
Chemical Substances	Antidepressive Agents ; Antipsychotic Agents ; Dopamine Uptake Inhibitors ; Phenols ; Piperazines ; Quinolones ; Receptors, Serotonin ; SB 269970 ; Serotonin Antagonists ; Sulfonamides ; serotonin 7 receptor ; Citalopram (0DHU5B8D6V) ; Aripiprazole (82VFR53I78) ; Corticosterone (W980KJ009P)
Language	English
Publishing date	2010-01-25
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	449927-x
ISSN	1872-7549 ; 0166-4328
ISSN (online)	1872-7549
ISSN	0166-4328
DOI	10.1016/j.bbr.2010.01.022
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Article ; Online: Host endothelial S1PR1 regulation of vascular permeability modulates tumor growth.

Sarkisyan, Gor / Gay, Laurie J / Nguyen, Nhan / Felding, Brunhilde H / Rosen, Hugh

American journal of physiology. Cell physiology

2014 Volume 307, Issue 1, Page(s) C14–24

Abstract: Understanding vascular growth and maturation in developing tumors has important implications for tumor progression, spread, and ultimately host survival. Modulating the signaling of endothelial G protein-coupled receptors (GPCRs) in blood and lymphatic ... ...

Abstract	Understanding vascular growth and maturation in developing tumors has important implications for tumor progression, spread, and ultimately host survival. Modulating the signaling of endothelial G protein-coupled receptors (GPCRs) in blood and lymphatic vessels can enhance or limit tumor progression. Sphingosine 1-phosphate receptor 1 (S1PR1) is a GPCR for circulating lysophospholipid S1P that is highly expressed in blood and lymphatic vessels. Using the S1PR1- enhanced green fluorescent protein (eGFP) mouse model in combination with intravital imaging and pharmacologic modulation of S1PR1 signaling, we show that boundary conditions of high and low S1PR1 signaling retard tumor progression by enhancing or destabilizing neovasculature integrity, respectively. In contrast, midrange S1PR1 signaling, achieved by receptor antagonist titration, promotes abundant growth of small, organized vessels and thereby enhances tumor progression. Furthermore, in vivo S1PR1 antagonism supports lung colonization by circulating tumor cells. Regulation of endothelial S1PR1 dynamically controls vascular integrity and maturation and thus modulates angiogenesis, tumor growth, and hematogenous metastasis.
MeSH term(s)	Anilides/pharmacology ; Animals ; Breast Neoplasms/blood supply ; Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Capillary Permeability/drug effects ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Endothelial Cells/pathology ; Female ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Lung Neoplasms/metabolism ; Lung Neoplasms/secondary ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Neoplastic Cells, Circulating/metabolism ; Neoplastic Cells, Circulating/pathology ; Neovascularization, Pathologic ; Organophosphonates/pharmacology ; Receptors, Lysosphingolipid/drug effects ; Receptors, Lysosphingolipid/genetics ; Receptors, Lysosphingolipid/metabolism ; Signal Transduction ; Sphingosine-1-Phosphate Receptors ; Time Factors ; Tumor Burden
Chemical Substances	3-amino-4-(3-hexylphenylamino)-4-oxobutylphosphonic acid ; Anilides ; Organophosphonates ; Receptors, Lysosphingolipid ; S1pr1 protein, mouse ; Sphingosine-1-Phosphate Receptors ; enhanced green fluorescent protein ; Green Fluorescent Proteins (147336-22-9)
Language	English
Publishing date	2014-04-16
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	392098-7
ISSN	1522-1563 ; 0363-6143
ISSN (online)	1522-1563
ISSN	0363-6143
DOI	10.1152/ajpcell.00043.2014
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Article ; Online: Real-time differential labeling of blood, interstitium, and lymphatic and single-field analysis of vasculature dynamics in vivo.

Sarkisyan, Gor / Cahalan, Stuart M / Gonzalez-Cabrera, Pedro J / Leaf, Nora B / Rosen, Hugh

American journal of physiology. Cell physiology

2012 Volume 302, Issue 10, Page(s) C1460–8

Abstract: Lymph nodes are highly organized structures specialized for efficient regulation of adaptive immunity. The blood and lymphatic systems within a lymph node play essential roles by providing functionally distinct environments for lymphocyte entry and ... ...

Abstract	Lymph nodes are highly organized structures specialized for efficient regulation of adaptive immunity. The blood and lymphatic systems within a lymph node play essential roles by providing functionally distinct environments for lymphocyte entry and egress, respectively. Direct imaging and measurement of vascular microenvironments by intravital multiphoton microscopy provide anatomical and mechanistic insights into the essential events of lymphocyte trafficking. Lymphocytes, blood endothelial cells, and lymphatic endothelial cells express sphingosine 1-phosphate receptor 1, a key G protein-coupled receptor regulating cellular egress and a modulator of endothelial permeability. Here we report the development of a differential vascular labeling (DVL) technique in which a single intravenous injection of a fluorescent dextran, in combination with fluorescent semiconductor quantum dot particles, differentially labels multiple blood and lymphatic compartments in a manner dependent on the size of the fluorescent particle used. Thus DVL allows measurement of endothelial integrity in multiple vascular compartments and the affects or pharmacological manipulation in vascular integrity. In addition, this technique allows for real-time observation of lymphocyte trafficking across physiological barriers differentiated by DVL. Last, single-field fluid movement dynamics can be derived, allowing for the simultaneous determination of fluid flow rates in diverse blood and lymphatic compartments.
MeSH term(s)	Animals ; Computer Systems ; Endothelial Cells/chemistry ; Endothelial Cells/physiology ; Extracellular Fluid/chemistry ; Extracellular Fluid/physiology ; Lymph Nodes/blood supply ; Lymph Nodes/chemistry ; Lymph Nodes/physiology ; Lymphatic Vessels/chemistry ; Lymphatic Vessels/physiology ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Receptors, Lysosphingolipid/biosynthesis ; Receptors, Lysosphingolipid/blood ; Regional Blood Flow/physiology ; Staining and Labeling/methods
Chemical Substances	Receptors, Lysosphingolipid
Language	English
Publishing date	2012-02-22
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	392098-7
ISSN	1522-1563 ; 0363-6143
ISSN (online)	1522-1563
ISSN	0363-6143
DOI	10.1152/ajpcell.00382.2011
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Article ; Online: The aged lymphoid tissue environment fails to support naïve T cell homeostasis.

Becklund, Bryan R / Purton, Jared F / Ramsey, Chris / Favre, Stéphanie / Vogt, Tobias K / Martin, Christopher E / Spasova, Darina S / Sarkisyan, Gor / LeRoy, Eric / Tan, Joyce T / Wahlus, Heidi / Bondi-Boyd, Brea / Luther, Sanjiv A / Surh, Charles D

Scientific reports

2016 Volume 6, Page(s) 30842

Abstract: Aging is associated with a gradual loss of naïve T cells and a reciprocal increase in the proportion of memory T cells. While reduced thymic output is important, age-dependent changes in factors supporting naïve T cells homeostasis may also be involved. ... ...

Abstract	Aging is associated with a gradual loss of naïve T cells and a reciprocal increase in the proportion of memory T cells. While reduced thymic output is important, age-dependent changes in factors supporting naïve T cells homeostasis may also be involved. Indeed, we noted a dramatic decrease in the ability of aged mice to support survival and homeostatic proliferation of naïve T cells. The defect was not due to a reduction in IL-7 expression, but from a combination of changes in the secondary lymphoid environment that impaired naïve T cell entry and access to key survival factors. We observed an age-related shift in the expression of homing chemokines and structural deterioration of the stromal network in T cell zones. Treatment with IL-7/mAb complexes can restore naïve T cell homeostatic proliferation in aged mice. Our data suggests that homeostatic mechanisms that support the naïve T cell pool deteriorate with age.
MeSH term(s)	Animals ; Cell Proliferation ; Homeostasis/physiology ; Immunologic Memory/immunology ; Lymphocyte Activation/immunology ; Lymphoid Tissue/immunology ; Mice ; Mice, Inbred C57BL ; T-Lymphocyte Subsets/immunology
Language	English
Publishing date	2016--02
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/srep30842
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Article ; Online: S1P(1) receptor modulation with cyclical recovery from lymphopenia ameliorates mouse model of multiple sclerosis.

Gonzalez-Cabrera, Pedro J / Cahalan, Stuart M / Nguyen, Nhan / Sarkisyan, Gor / Leaf, Nora B / Cameron, Michael D / Kago, Tomoyuki / Rosen, Hugh

Molecular pharmacology

2011 Volume 81, Issue 2, Page(s) 166–174

Abstract: Multiple sclerosis (MS) therapies modulate T-cell autoimmunity in the central nervous system (CNS) but may exacerbate latent infections. Fingolimod, a nonselective sphingosine-1-phosphate (S1P) receptor agonist that induces sustained lymphopenia and ... ...

Abstract	Multiple sclerosis (MS) therapies modulate T-cell autoimmunity in the central nervous system (CNS) but may exacerbate latent infections. Fingolimod, a nonselective sphingosine-1-phosphate (S1P) receptor agonist that induces sustained lymphopenia and accumulates in the CNS, represents a new treatment modality for MS. We hypothesized that sustained lymphopenia would not be required for efficacy and that a selective, CNS-penetrant, peripherally short-acting, S1P(1) agonist would show full efficacy in a mouse MS model. Using daily treatment with 10 mg/kg 2-(4-(5-(3,4-diethoxyphenyl)-1,2,4-oxadiazol-3-yl)-2,3-dihydro-1H-inden-1-yl amino)ethanol (CYM-5442) at the onset of clinical signs in myelin oligodendrocyte glycoprotein MOG(35-55)- induced experimental allergic encephalomyelitis (EAE), we assessed clinical scores, CNS cellular infiltration, demyelination, and gliosis for 12 days with CYM-5442, vehicle, or fingolimod. CYM-5442 levels in CNS and plasma were determined at experiment termination, and blood lymphopenia was measured 3 and 24 h after the last injection. Plasma levels of cytokines were assayed at the end of the protocol. Changes in S1P(1)-enhanced green fluorescent protein expression on neurons and astrocytes during active EAE and upon CYM-5442 treatment were quantified with flow cytometry and Western blotting by using native-locus enhanced green fluorescent protein-tagged S1P(1) mice. S1P(1) agonism alone reduced pathological features as did fingolimod (maximally lymphopenic throughout), despite full reversal of lymphopenia within each dosing interval. CYM-5442 levels in CNS but not in plasma were sustained. Neuronal and astrocytic S1P(1) expression in EAE was suppressed by CYM-5442 treatment, relative to vehicle, and levels of key cytokines, such as interleukin 17A, were also significantly reduced in drug-treated mice. S1P(1)-selective agonists that induce reversible lymphopenia while persisting in the CNS may be effective MS treatments.
MeSH term(s)	Animals ; Central Nervous System/metabolism ; Cytokines/drug effects ; Disease Models, Animal ; Encephalomyelitis, Autoimmune, Experimental/drug therapy ; Fingolimod Hydrochloride ; Immunosuppressive Agents ; Indans ; Lymphopenia/drug therapy ; Mice ; Multiple Sclerosis/drug therapy ; Oxadiazoles ; Propylene Glycols/therapeutic use ; Receptors, Lysosphingolipid/agonists ; Receptors, Lysosphingolipid/metabolism ; Sphingosine/analogs & derivatives ; Sphingosine/therapeutic use
Chemical Substances	2-(4-(5-(3,4-diethoxyphenyl)-1,2,4-oxadiazol-3-yl)-2,3-dihydro-1H-inden-1-yl amino)ethanol ; Cytokines ; Immunosuppressive Agents ; Indans ; Oxadiazoles ; Propylene Glycols ; Receptors, Lysosphingolipid ; Fingolimod Hydrochloride (G926EC510T) ; Sphingosine (NGZ37HRE42)
Language	English
Publishing date	2011-10-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	124034-1
ISSN	1521-0111 ; 0026-895X
ISSN (online)	1521-0111
ISSN	0026-895X
DOI	10.1124/mol.111.076109
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Zs.A 525: Show issues

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Article ; Online: LP-211 is a brain penetrant selective agonist for the serotonin 5-HT(7) receptor.

Hedlund, Peter B / Leopoldo, Marcello / Caccia, Silvio / Sarkisyan, Gor / Fracasso, Claudia / Martelli, Giuliana / Lacivita, Enza / Berardi, Francesco / Perrone, Roberto

Neuroscience letters

2010 Volume 481, Issue 1, Page(s) 12–16

Abstract: We have determined the pharmacological profile of the new serotonin 5-HT(7) receptor agonist N-(4-cyanophenylmethyl)-4-(2-diphenyl)-1-piperazinehexanamide (LP-211). Radioligand binding assays were performed on a panel of 5-HT receptor subtypes. The ... ...

Abstract	We have determined the pharmacological profile of the new serotonin 5-HT(7) receptor agonist N-(4-cyanophenylmethyl)-4-(2-diphenyl)-1-piperazinehexanamide (LP-211). Radioligand binding assays were performed on a panel of 5-HT receptor subtypes. The compound was also evaluated in vivo by examining its effect on body temperature regulation in mice lacking the 5-HT(7) receptor (5-HT(7)(-/-)) and their 5-HT(7)(+/+) sibling controls. Disposition studies were performed in mice of both genotypes. It was found that LP-211 was brain penetrant and underwent metabolic degradation to 1-(2-diphenyl)piperazine (RA-7). In vitro binding assays revealed that RA-7 possessed higher 5-HT(7) receptor affinity than LP-211 and a better selectivity profile over a panel of 5-HT receptor subtypes. In vivo it was demonstrated that LP-211, and to a lesser degree RA-7, induced hypothermia in 5-HT(7)(+/+) but not in 5-HT(7)(-/-) mice. Our results suggest that LP-211 can be used as a 5-HT(7) receptor agonist in vivo.
MeSH term(s)	Animals ; Body Temperature/drug effects ; Body Temperature/genetics ; Brain/anatomy & histology ; Brain/drug effects ; Brain/metabolism ; Dose-Response Relationship, Drug ; Humans ; Male ; Mice ; Mice, Knockout ; Phenylcarbamates/chemistry ; Phenylcarbamates/metabolism ; Phenylcarbamates/pharmacology ; Piperazines/chemistry ; Piperazines/metabolism ; Piperazines/pharmacology ; Protein Binding/drug effects ; Protein Binding/genetics ; Receptors, Serotonin/deficiency ; Receptors, Serotonin/metabolism ; Serotonin Receptor Agonists/chemistry ; Serotonin Receptor Agonists/metabolism ; Serotonin Receptor Agonists/pharmacology ; Urethane/analogs & derivatives ; Urethane/chemistry ; Urethane/metabolism ; Urethane/pharmacology
Chemical Substances	N-(4-cyanophenylmethyl)-4-(2-diphenyl)-1-piperazinehexanamide ; Phenylcarbamates ; Piperazines ; Receptors, Serotonin ; Serotonin Receptor Agonists ; serotonin 7 receptor ; N,N-methylethylphenylcarbamate (135467-93-5) ; Urethane (3IN71E75Z5)
Language	English
Publishing date	2010-06-23
Publishing country	Ireland
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	194929-9
ISSN	1872-7972 ; 0304-3940
ISSN (online)	1872-7972
ISSN	0304-3940
DOI	10.1016/j.neulet.2010.06.036
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Article ; Online: The CD100 receptor interacts with its plexin B2 ligand to regulate epidermal γδ T cell function.

Witherden, Deborah A / Watanabe, Megumi / Garijo, Olivia / Rieder, Stephanie E / Sarkisyan, Gor / Cronin, Shane J F / Verdino, Petra / Wilson, Ian A / Kumanogoh, Atsushi / Kikutani, Hitoshi / Teyton, Luc / Fischer, Wolfgang H / Havran, Wendy L

Immunity

2012 Volume 37, Issue 2, Page(s) 314–325

Abstract: γδ T cells respond rapidly to keratinocyte damage, providing essential contributions to the skin wound healing process. The molecular interactions regulating their response are unknown. Here, we identify a role for interaction of plexin B2 with the CD100 ...

Abstract	γδ T cells respond rapidly to keratinocyte damage, providing essential contributions to the skin wound healing process. The molecular interactions regulating their response are unknown. Here, we identify a role for interaction of plexin B2 with the CD100 receptor in epithelial repair. In vitro blocking of plexin B2 or CD100 inhibited γδ T cell activation. Furthermore, CD100 deficiency in vivo resulted in delayed repair of cutaneous wounds due to a disrupted γδ T cell response to keratinocyte damage. Ligation of CD100 in γδ T cells induced cellular rounding via signals through ERK kinase and cofilin. Defects in this rounding process were evident in the absence of CD100-mediated signals, thereby providing a mechanistic explanation for the defective wound healing in CD100-deficient animals. The discovery of immune functions for plexin B2 and CD100 provides insight into the complex cell-cell interactions between epithelial resident γδ T cells and the neighboring cells they support.
MeSH term(s)	Actin Depolymerizing Factors/metabolism ; Animals ; Antigens, CD/immunology ; Antigens, CD/metabolism ; CHO Cells ; Cell Communication/immunology ; Cell Shape ; Cricetinae ; Epidermis/immunology ; Epidermis/injuries ; Extracellular Signal-Regulated MAP Kinases/metabolism ; HEK293 Cells ; Humans ; Keratinocytes/immunology ; Keratinocytes/metabolism ; Langerhans Cells/immunology ; Langerhans Cells/metabolism ; Lymphocyte Activation/immunology ; Mass Spectrometry ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Nerve Tissue Proteins/immunology ; Nerve Tissue Proteins/metabolism ; Phosphorylation ; Protein Binding/immunology ; Receptors, Antigen, T-Cell, gamma-delta/immunology ; Receptors, Antigen, T-Cell, gamma-delta/metabolism ; Semaphorins/immunology ; Semaphorins/metabolism ; Sequence Analysis, Protein ; Surface Plasmon Resonance ; T-Lymphocytes/immunology ; T-Lymphocytes/metabolism ; Wound Healing/immunology
Chemical Substances	Actin Depolymerizing Factors ; Antigens, CD ; CD100 antigen ; Nerve Tissue Proteins ; Plxnb2 protein, mouse ; Receptors, Antigen, T-Cell, gamma-delta ; Semaphorins ; Extracellular Signal-Regulated MAP Kinases (EC 2.7.11.24)
Language	English
Publishing date	2012-08-16
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1217235-2
ISSN	1097-4180 ; 1074-7613
ISSN (online)	1097-4180
ISSN	1074-7613
DOI	10.1016/j.immuni.2012.05.026
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Zs.MG 69: Show issues

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Article ; Online: Actions of a picomolar short-acting S1P₁ agonist in S1P₁-eGFP knock-in mice.

Cahalan, Stuart M / Gonzalez-Cabrera, Pedro J / Sarkisyan, Gor / Nguyen, Nhan / Schaeffer, Marie-Therese / Huang, Liming / Yeager, Adam / Clemons, Bryan / Scott, Fiona / Rosen, Hugh

Nature chemical biology

2011 Volume 7, Issue 5, Page(s) 254–256

Abstract: Sphingosine 1-phosphate receptor 1 (S1P(1)) is critical for lymphocyte recirculation and is a clinical target for treatment of multiple sclerosis. By generating a short-duration S1P(1) agonist and mice in which fluorescently tagged S1P(1) replaces wild- ... ...

Abstract	Sphingosine 1-phosphate receptor 1 (S1P(1)) is critical for lymphocyte recirculation and is a clinical target for treatment of multiple sclerosis. By generating a short-duration S1P(1) agonist and mice in which fluorescently tagged S1P(1) replaces wild-type receptor, we elucidate physiological and agonist-perturbed changes in expression of S1P(1) at a subcellular level in vivo. We demonstrate differential downregulation of S1P(1) on lymphocytes and endothelia after agonist treatment.
MeSH term(s)	Animals ; Down-Regulation/drug effects ; Endothelium/drug effects ; Endothelium/metabolism ; Flow Cytometry ; Fluorescent Dyes/chemistry ; Fluorescent Dyes/metabolism ; Gene Knock-In Techniques ; Green Fluorescent Proteins/chemistry ; Green Fluorescent Proteins/metabolism ; Lymphocytes/drug effects ; Lymphocytes/metabolism ; Mice ; Multiple Sclerosis/drug therapy ; Multiple Sclerosis/metabolism ; Multiple Sclerosis/pathology ; Receptors, Lysosphingolipid/agonists ; Receptors, Lysosphingolipid/metabolism ; Receptors, Lysosphingolipid/therapeutic use ; Time Factors
Chemical Substances	Fluorescent Dyes ; Receptors, Lysosphingolipid ; enhanced green fluorescent protein ; Green Fluorescent Proteins (147336-22-9)
Language	English
Publishing date	2011-03-27
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2202962-X
ISSN	1552-4469 ; 1552-4450
ISSN (online)	1552-4469
ISSN	1552-4450
DOI	10.1038/nchembio.547
Database	MEDical Literature Analysis and Retrieval System OnLINE

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