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Article: [Expert consensus statement on Pudilan Xiaoyan Oral Liquid in clinical practice].

Wang, Lian-Xin / Miao, Qing / Xie, Yan-Ming / Chen, Da-Can / Sun, Su-Lun / Zhang, Hong-Chun / Jia, Zhong-Wu / Li, Tie-Nan / Zhu, Jia / Shi, Li-Qing / Song, Ping / Gao, Feng / Wei, Bao-Lin / Feng, Cui-Ling / Qu, Yi-Qing / Qu, Ni-Ni / Yu, Xue-Feng / Zhang, Nian-Zhi / Yu, Xue-Qing

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2020 Volume 44, Issue 24, Page(s) 5277–5281

Abstract: Pudilan Xiaoyan Oral Liquid has effects in clearing away heat and detoxifying,and is used to treat ...

Abstract	Pudilan Xiaoyan Oral Liquid has effects in clearing away heat and detoxifying,and is used to treat pharynx and throat swelling caused by the syndrome of excessive heat and toxin accumulation. Its efficacy is to relieve swelling and pain( redness,swelling and hot pain). It is included in the Chinese Pharmacopoeia of 2015 Edition,and has been listed in provincial health insurance directories of Shaanxi,Jiangsu,Liaoning,Hunan,Tianjin,Xinjiang and Hebei. It has been recommended by health departments of Beijing,Chongqing and other provinces as a preferred drug for the prevention and treatment of H1 N1 and HFMD,and listed in the diagnosis and Treatment Guide of HFMD by the Ministry of Health,the Clinical Application Guide of Chinese Patent Medicine edited by the Lung Department Disease Branch of China Association of Chinese Medicine,and the Clinical Practice Guide of Single Administration/Combined Administration of Antibiotics in Treatment of Common Infectious Diseases by China Association of Chinese Medicine. To further improve the clinician's understanding of drugs and better guide the rational clinical application,we invited front-line clinical experts from respiratory department,infectious department and dermatology of traditional Chinese and Western medicine to develop and compile the expert consensus. The consensus fully considered the clinical evidence and the expert clinical experience to give recommendations for clinical problems with evidence support and consensus suggestions for clinical problems without evidence support by the nominal group method.This consensus is based on clinical research evidence and expert experience in a simple and clear format,which provides a preliminary reference for the clinical use of the drug.
MeSH term(s)	China ; Consensus ; Drugs, Chinese Herbal/therapeutic use ; Humans ; Medicine, Chinese Traditional ; Nonprescription Drugs
Chemical Substances	Drugs, Chinese Herbal ; Nonprescription Drugs ; Pudilan xiaoyan
Language	Chinese
Publishing date	2020-04-01
Publishing country	China
Document type	Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.19540/j.cnki.cjcmm.20191105.501
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Systematic identification of the interventional mechanism of Qingfei Xiaoyan Wan (QFXYW) in treatment of the cytokine storm in acute lung injury using transcriptomics-based system pharmacological analyses.

Hou, Jing-Yi / Wu, Jia-Rong / Chen, Yi-Bing / Xu, Dong / Liu, Shu / Shang, Dan-Dan / Fan, Guan-Wei / Cui, Yuan-Lu

Pharmaceutical biology

2022 Volume 60, Issue 1, Page(s) 743–754

Abstract: ... and there is no specific and effective treatment for ALI. Qingfei Xiaoyan Wan (QFXYW) has been widely ...

Abstract	Context: Acute lung injury (ALI) is a complex, severe inflammation disease with high mortality, and there is no specific and effective treatment for ALI. Qingfei Xiaoyan Wan (QFXYW) has been widely used to treat lung-related diseases for centuries. Objective: This study evaluates the potential effects and elucidates the therapeutic mechanism of QFXYW against LPS induced ALI in mice. Materials and methods: BALB/c Mice in each group were first orally administered medicines (0.9% saline solution for the control group, 0.5 mg/kg Dexamethasone, or 1.3, 2.6, 5.2 g/kg QFXYW), after 4 h, the groups were injected LPS (1.0 mg/kg) to induce ALI, then the same medicines were administered repeatedly. The transcriptomics-based system pharmacological analyses were applied to screen the hub genes, RT-PCR, ELISA, and protein array assay was applied to verify the predicted hub genes and key pathways. Results: QFXYW significantly decreased the number of leukocytes from (6.34 ± 0.51) × 10 Conclusions: This study showed that QFXYW decreased the number of leukocytes and neutrophils by attenuating inflammatory response, which provides an important basis for the use of QFXYW in the treatment of ALI.
MeSH term(s)	Acute Lung Injury/chemically induced ; Acute Lung Injury/drug therapy ; Acute Lung Injury/metabolism ; Animals ; Cytokine Release Syndrome ; Lipopolysaccharides/pharmacology ; Mice ; Mice, Inbred BALB C ; Transcriptome
Chemical Substances	Lipopolysaccharides
Language	English
Publishing date	2022-03-31
Publishing country	England
Document type	Journal Article
ZDB-ID	1440131-9
ISSN	1744-5116 ; 1388-0209
ISSN (online)	1744-5116
ISSN	1388-0209
DOI	10.1080/13880209.2022.2055090
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Article ; Online: Integration of transcriptomics and system pharmacology to reveal the therapeutic mechanism underlying Qingfei Xiaoyan Wan to treat allergic asthma.

Hou, Jing-Yi / Wu, Jia-Rong / Xu, Dong / Chen, Yi-Bing / Shang, Dan-Dan / Liu, Shu / Fan, Guan-Wei / Cui, Yuan-Lu

Journal of ethnopharmacology

2021 Volume 278, Page(s) 114302

Abstract: ... inflammation, hyperresponsiveness, and bronchial smooth muscle contraction. Qingfei Xiaoyan Wan (QFXYW ...

Abstract	Ethnopharmacological relevance: Asthma is a chronic inflammatory disease, characterized by airway inflammation, hyperresponsiveness, and bronchial smooth muscle contraction. Qingfei Xiaoyan Wan (QFXYW), a traditional Chinese formula, has been shown to exert anti-asthma effects and immune response in multiple diseases. Aim of this study: In this study, we evaluated the therapeutic mechanism of QFXYW in the suppression of allergic asthma by integrating of transcriptomics and system pharmacology. Materials and methods: BALB/c mice were sensitized with ovalbumin (OVA) to establish the allergic asthma model, and its success was confirmed with behavioral observations. Lung histopathological analysis, inflammatory pathology scores, transcription factors were used to evaluate the effects of QFXYW on allergic asthma. The therapeutic mechanism of QFXYW in treating allergic asthma through integrated transcriptomics and system pharmacology was then determined: hub genes were screened out by topological analysis and functional enrichment analysis were performed to identify key signaling pathway. Subsequently, quantitative RP-PCR and protein array were performed to detect the mRNA of hub genes and to predict the key pathway in OVA-induced allergic asthma, respectively. Results: Our results demonstrated that QFXYW could significantly attenuate inflammatory cell infiltration, mucus secretion, and epithelial damage. The transcriptomics analysis found the six hub genes with the highest values- CXCL10, CXCL2, CXCL1, IL-6, CCL-5, and CCL-4 were screened out. Functional enrichment analysis showed that the differentially expressed genes (DEGs) were mainly enriched in the inflammatory response and cytokine signaling pathway. Moreover, the quantitative RT-PCR verification experiment found the CXCL2 and CXCL1 were significantly suppressed after treatment with QFXYW. The results of protein array showed that QFXYW inhibited the multi-cytokines of OVA-induced allergic asthma via cytokine signaling pathway. Conclusions: QFXYW may have mediated OVA-induced allergic asthma mainly through the hub genes CXCL2, CXCL1, and the cytokine signaling pathway. This finding will offer a novel strategy to explore effective and safe mechanism of Traditional Chinese Medicine (TCM) formula to treat allergic asthma.
MeSH term(s)	Animals ; Anti-Asthmatic Agents/therapeutic use ; Asthma/chemically induced ; Asthma/drug therapy ; Cytokines/genetics ; Cytokines/metabolism ; Drugs, Chinese Herbal/therapeutic use ; Female ; Gene Expression Regulation/drug effects ; Gene Expression Regulation/immunology ; Hypersensitivity/drug therapy ; Lung/drug effects ; Lung/metabolism ; Mice ; Mice, Inbred BALB C ; Ovalbumin/toxicity ; Transcriptome
Chemical Substances	Anti-Asthmatic Agents ; Cytokines ; Drugs, Chinese Herbal ; Ovalbumin (9006-59-1)
Language	English
Publishing date	2021-06-04
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2021.114302
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Systematic identification of the interventional mechanism of Qingfei Xiaoyan Wan (QFXYW) in treatment of the cytokine storm in acute lung injury using transcriptomics-based system pharmacological analyses

Hou, Jing-Yi / Wu, Jia-Rong / Chen, Yi-Bing / Xu, Dong / Liu, Shu / Shang, Dan-dan / Fan, Guan-Wei / Cui, Yuan-Lu

Pharmaceutical Biology. 2022 Dec. 31, v. 60, no. 1 p.743-754

2022

Abstract: ... no specific and effective treatment for ALI. Qingfei Xiaoyan Wan (QFXYW) has been widely used ...

Abstract	Acute lung injury (ALI) is a complex, severe inflammation disease with high mortality, and there is no specific and effective treatment for ALI. Qingfei Xiaoyan Wan (QFXYW) has been widely used to treat lung-related diseases for centuries. This study evaluates the potential effects and elucidates the therapeutic mechanism of QFXYW against LPS induced ALI in mice. BALB/c Mice in each group were first orally administered medicines (0.9% saline solution for the control group, 0.5 mg/kg Dexamethasone, or 1.3, 2.6, 5.2 g/kg QFXYW), after 4 h, the groups were injected LPS (1.0 mg/kg) to induce ALI, then the same medicines were administered repeatedly. The transcriptomics-based system pharmacological analyses were applied to screen the hub genes, RT-PCR, ELISA, and protein array assay was applied to verify the predicted hub genes and key pathways. QFXYW significantly decreased the number of leukocytes from (6.34 ± 0.51) × 10⁵/mL to (4.01 ± 0.11) × 10⁵/mL, accompanied by the neutrophil from (1.41 ± 0.19) × 10⁵/mL to (0.77 ± 0.10) × 10⁵/mL in bronchoalveolar lavage fluid (BALF). Based on Degree of node connection (Degree) and BottleNeck (BN), important parameters of network topology, the protein-protein interaction (PPI) network screened hub genes, including IL-6, TNF-α, CCL2, TLR2, CXCL1, and MMP-9. The results of RT-PCR, ELISA, and protein chip assay revealed that QFXYW could effectively inhibit ALI via multiple key targets and the cytokine-cytokine signalling pathway. This study showed that QFXYW decreased the number of leukocytes and neutrophils by attenuating inflammatory response, which provides an important basis for the use of QFXYW in the treatment of ALI.
Keywords	dexamethasone ; inflammation ; interleukin-6 ; lungs ; mortality ; neutrophils ; oral administration ; protein microarrays ; protein-protein interactions ; sodium chloride ; transcriptomics ; Anti-inflammatory ; traditional Chinese medicine ; protein array analysis ; protein-protein interaction network
Language	English
Dates of publication	2022-1231
Size	p. 743-754.
Publishing place	Taylor & Francis
Document type	Article ; Online
ZDB-ID	1440131-9
ISSN	1744-5116 ; 1388-0209
ISSN (online)	1744-5116
ISSN	1388-0209
DOI	10.1080/13880209.2022.2055090
Database	NAL-Catalogue (AGRICOLA)

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Article: [Source control of Pudilan Xiaoyan Oral Liquid--research on quality of Taraxaci Herba based on ultra high performance liquid chromatography fingerprint].

Chao, L I / Xiao-Nan, S U / Fa-Gen, Zhu / Fei, W U / Wei-Quan, B U / Liang, Feng / Xiao-Bin, Jia / Meng-Hua, Jiang / E, Sun

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2020 Volume 45, Issue 18, Page(s) 4307–4315

Abstract: Pudilan Xiaoyan Oral Liquid has been widely used in the clinical treatment of inflammatory diseases ... such as upper respiratory tract infections. Taraxaci Herba, as the monarch medicine in Pudilan Xiaoyan Oral ... among different batches of Pudilan Xiaoyan Oral Liquid. In this paper, Thermo Scientific Vanquish ultra-high ...

Abstract	Pudilan Xiaoyan Oral Liquid has been widely used in the clinical treatment of inflammatory diseases such as upper respiratory tract infections. Taraxaci Herba, as the monarch medicine in Pudilan Xiaoyan Oral Liquid, due to its multi-source, multi-origin characteristics, and the difference in the content of active ingredients in different medicinal parts, has become a potential factor for the unstable quality among different batches of Pudilan Xiaoyan Oral Liquid. In this paper, Thermo Scientific Vanquish ultra-high-performance liquid chromatography(UPLC) system was used, and the Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2012 Edition) issued by National Pharmacopoeia Commission was used for processing and analysis. The main common peaks were identified and contents were determined by comparison with reference substances. Fingerprints of Taraxaci Herba medicinal materials from different origins were established. 13 common peaks were identified, and 29 batches of samples from five origins had similarities above 0.90. At the same time, an ultra-high performance liquid chromatography method was developed for the determination of monocaffeoyl tartaric acid, chlorogenic acid, caffeic acid, chicoic acid, and luteolin in Taraxaci Herba. The quantitative analysis conditions were verified by methodology, and the average sample recovery was 97.30%-101.8%. The results showed that the content of the same ingredient in Taraxaci Herba from different origins and different medicinal parts was obviously different, and the fluctua-tion range was also different for different ingredients. The establishment of UPLC fingerprints for Taraxaci Herba from different regions combined with multi-component content determination methods provides a reference for improving the quality control of Taraxaci Herba medicinal materials, and also provides a source guarantee for the quality improvement of Pudilan Xiaoyan Oral Liquid.
MeSH term(s)	Chlorogenic Acid ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; Quality Control
Chemical Substances	Drugs, Chinese Herbal ; Pudilan xiaoyan ; Chlorogenic Acid (318ADP12RI)
Language	Chinese
Publishing date	2020-11-08
Publishing country	China
Document type	Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.19540/j.cnki.cjcmm.20200802.331
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Systematic identification of the interventional mechanism of Qingfei Xiaoyan Wan (QFXYW) in treatment of the cytokine storm in acute lung injury using transcriptomics-based system pharmacological analyses

Jing-Yi Hou / Jia-Rong Wu / Yi-Bing Chen / Dong Xu / Shu Liu / Dan-dan Shang / Guan-Wei Fan / Yuan-Lu Cui

Pharmaceutical Biology, Vol 60, Iss 1, Pp 743-

2022 Volume 754

Abstract: ... there is no specific and effective treatment for ALI. Qingfei Xiaoyan Wan (QFXYW) has been widely used ...

Abstract	Context Acute lung injury (ALI) is a complex, severe inflammation disease with high mortality, and there is no specific and effective treatment for ALI. Qingfei Xiaoyan Wan (QFXYW) has been widely used to treat lung-related diseases for centuries.Objective This study evaluates the potential effects and elucidates the therapeutic mechanism of QFXYW against LPS induced ALI in mice.Materials and methods BALB/c Mice in each group were first orally administered medicines (0.9% saline solution for the control group, 0.5 mg/kg Dexamethasone, or 1.3, 2.6, 5.2 g/kg QFXYW), after 4 h, the groups were injected LPS (1.0 mg/kg) to induce ALI, then the same medicines were administered repeatedly. The transcriptomics-based system pharmacological analyses were applied to screen the hub genes, RT-PCR, ELISA, and protein array assay was applied to verify the predicted hub genes and key pathways.Results QFXYW significantly decreased the number of leukocytes from (6.34 ± 0.51) × 105/mL to (4.01 ± 0.11) × 105/mL, accompanied by the neutrophil from (1.41 ± 0.19) × 105/mL to (0.77 ± 0.10) × 105/mL in bronchoalveolar lavage fluid (BALF). Based on Degree of node connection (Degree) and BottleNeck (BN), important parameters of network topology, the protein-protein interaction (PPI) network screened hub genes, including IL-6, TNF-α, CCL2, TLR2, CXCL1, and MMP-9. The results of RT-PCR, ELISA, and protein chip assay revealed that QFXYW could effectively inhibit ALI via multiple key targets and the cytokine-cytokine signalling pathway.Conclusions This study showed that QFXYW decreased the number of leukocytes and neutrophils by attenuating inflammatory response, which provides an important basis for the use of QFXYW in the treatment of ALI.
Keywords	Anti-inflammatory ; traditional Chinese medicine ; protein array analysis ; protein-protein interaction network ; Therapeutics. Pharmacology ; RM1-950
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	Taylor & Francis Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Integration of transcriptomics and system pharmacology to reveal the therapeutic mechanism underlying Qingfei Xiaoyan Wan to treat allergic asthma

Hou, Jing-Yi / Wu, Jia-Rong / Xu, Dong / Chen, Yi-Bing / Shang, Dan-Dan / Liu, Shu / Fan, Guan-Wei / Cui, Yuan-Lu

Journal of ethnopharmacology. 2021 Oct. 05, v. 278

2021

Abstract: ... and bronchial smooth muscle contraction. Qingfei Xiaoyan Wan (QFXYW), a traditional Chinese formula ...

Abstract	Asthma is a chronic inflammatory disease, characterized by airway inflammation, hyperresponsiveness, and bronchial smooth muscle contraction. Qingfei Xiaoyan Wan (QFXYW), a traditional Chinese formula, has been shown to exert anti-asthma effects and immune response in multiple diseases.In this study, we evaluated the therapeutic mechanism of QFXYW in the suppression of allergic asthma by integrating of transcriptomics and system pharmacology.BALB/c mice were sensitized with ovalbumin (OVA) to establish the allergic asthma model, and its success was confirmed with behavioral observations. Lung histopathological analysis, inflammatory pathology scores, transcription factors were used to evaluate the effects of QFXYW on allergic asthma. The therapeutic mechanism of QFXYW in treating allergic asthma through integrated transcriptomics and system pharmacology was then determined: hub genes were screened out by topological analysis and functional enrichment analysis were performed to identify key signaling pathway. Subsequently, quantitative RP-PCR and protein array were performed to detect the mRNA of hub genes and to predict the key pathway in OVA-induced allergic asthma, respectively.Our results demonstrated that QFXYW could significantly attenuate inflammatory cell infiltration, mucus secretion, and epithelial damage. The transcriptomics analysis found the six hub genes with the highest values- CXCL10, CXCL2, CXCL1, IL-6, CCL-5, and CCL-4 were screened out. Functional enrichment analysis showed that the differentially expressed genes (DEGs) were mainly enriched in the inflammatory response and cytokine signaling pathway. Moreover, the quantitative RT-PCR verification experiment found the CXCL2 and CXCL1 were significantly suppressed after treatment with QFXYW. The results of protein array showed that QFXYW inhibited the multi-cytokines of OVA-induced allergic asthma via cytokine signaling pathway.QFXYW may have mediated OVA-induced allergic asthma mainly through the hub genes CXCL2, CXCL1, and the cytokine signaling pathway. This finding will offer a novel strategy to explore effective and safe mechanism of Traditional Chinese Medicine (TCM) formula to treat allergic asthma.
Keywords	Oriental traditional medicine ; asthma ; epithelium ; gene expression regulation ; histopathology ; immune response ; inflammation ; interleukin-6 ; lungs ; models ; mucus ; muscle contraction ; ovalbumin ; pharmacology ; secretion ; smooth muscle ; therapeutics ; topology ; transcriptomics
Language	English
Dates of publication	2021-1005
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2021.114302
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Qingfei Xiaoyan Wan alleviates asthma through multi-target network regulation.

Zhao, Zhenying / Miao, Yingbo / Pan, Pengwei / Cheng, Binfeng / Bai, Gang / Wu, Hong

BMC complementary and alternative medicine

2013 Volume 13, Page(s) 206

Abstract: Background: Qingfei Xiaoyan Wan (QFXY), a traditional Chinese formula, is widely used ...

Abstract	Background: Qingfei Xiaoyan Wan (QFXY), a traditional Chinese formula, is widely used for relieving cough, asthma, upper respiratory tract infection, bronchitis, pneumonia, and etc. in clinic. Comparing with other anti-asthma drugs, it is characterised with moderate and persistent efficacy as well as few side effects, however, the underlying action mechanism still remains elusive. This study aimed to identify QFXY multi-target network regulation as an asthma controller. Methods: This study established asthma model induced by histamine phosphate and acetylcholine chloride (His&Ach) in guinea pigs, which then were administered orally with QFXY. Hematoxylin-Eosin staining sections were applied for evaluating QFXY effect. In both Model and QFXY groups, customized microarrays and 2D electrophoresis were adopted to detect differentially expressed genes (diff genes) and proteins (diff proteins) respectively, and some diff proteins were identified with MALDI-TOF/MS. The checked diff genes and proteins underwent Cluster, GO and KEGG analysis. Based on GAD and HPRD databases, QFXY-asthma target regulation network was constructed. Results: His&Ach-induced asthma model of guinea pigs was established. HE sections presented anti-inflammation and anti-remodelling effects of QFXY. Comparing with the Model group, 55 diff genes and 6 diff proteins were identified in QFXY group. Validation by qPCR and Western blot showed the microarray and 2D data reliable. Furthermore, QFXY-asthma target regulation network was achieved. Conclusions: A primarily combined genomic and proteomic screening of QFXY targets displayed a series of candidate genes and proteins, which indicated that the effect of QFXY relied on the combined mechanism, anti-inflammation and anti-remodelling, as well as influencing signal transduction in vivo.
MeSH term(s)	Acetylcholine/toxicity ; Animals ; Asthma/chemically induced ; Asthma/genetics ; Asthma/metabolism ; Asthma/therapy ; Cluster Analysis ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Electrophoresis, Gel, Two-Dimensional ; Female ; Gene Expression/drug effects ; Gene Regulatory Networks/genetics ; Gene Regulatory Networks/physiology ; Guinea Pigs ; Histamine/analogs & derivatives ; Histamine/toxicity ; Lung/chemistry ; Lung/drug effects ; Lung/metabolism ; Lung/pathology ; Male ; Oligonucleotide Array Sequence Analysis ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical Substances	Drugs, Chinese Herbal ; Histamine (820484N8I3) ; Acetylcholine (N9YNS0M02X) ; histamine phosphate (QWB37T4WZZ)
Language	English
Publishing date	2013-08-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2050429-9
ISSN	1472-6882 ; 1472-6882
ISSN (online)	1472-6882
ISSN	1472-6882
DOI	10.1186/1472-6882-13-206
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Qingfei Xiaoyan Wan alleviates asthma through multi-target network regulation

Zhao, Zhenying / Miao, Yingbo / Pan, Pengwei / Cheng, Binfeng / Bai, Gang / Wu, Hong

BMC Complement Altern Med. 2013 Dec., v. 13, no. 1 p.206-206

2013

Abstract: BACKGROUND: Qingfei Xiaoyan Wan (QFXY), a traditional Chinese formula, is widely used for relieving ...

Abstract	BACKGROUND: Qingfei Xiaoyan Wan (QFXY), a traditional Chinese formula, is widely used for relieving cough, asthma, upper respiratory tract infection, bronchitis, pneumonia, and etc. in clinic. Comparing with other anti-asthma drugs, it is characterised with moderate and persistent efficacy as well as few side effects, however, the underlying action mechanism still remains elusive. This study aimed to identify QFXY multi-target network regulation as an asthma controller. METHODS: This study established asthma model induced by histamine phosphate and acetylcholine chloride (His&Ach) in guinea pigs, which then were administered orally with QFXY. Hematoxylin-Eosin staining sections were applied for evaluating QFXY effect. In both Model and QFXY groups, customized microarrays and 2D electrophoresis were adopted to detect differentially expressed genes (diff genes) and proteins (diff proteins) respectively, and some diff proteins were identified with MALDI-TOF/MS. The checked diff genes and proteins underwent Cluster, GO and KEGG analysis. Based on GAD and HPRD databases, QFXY-asthma target regulation network was constructed. RESULTS: His&Ach-induced asthma model of guinea pigs was established. HE sections presented anti-inflammation and anti-remodelling effects of QFXY. Comparing with the Model group, 55 diff genes and 6 diff proteins were identified in QFXY group. Validation by qPCR and Western blot showed the microarray and 2D data reliable. Furthermore, QFXY-asthma target regulation network was achieved. CONCLUSIONS: A primarily combined genomic and proteomic screening of QFXY targets displayed a series of candidate genes and proteins, which indicated that the effect of QFXY relied on the combined mechanism, anti-inflammation and anti-remodelling, as well as influencing signal transduction in vivo.
Keywords	Western blotting ; acetylcholine ; asthma ; bronchitis ; chlorides ; complement ; cough ; electrophoresis ; gene expression regulation ; genomics ; histamine ; microarray technology ; models ; phosphates ; pneumonia ; proteomics ; signal transduction
Language	English
Dates of publication	2013-12
Size	p. 206.
Publishing place	BioMed Central
Document type	Article ; Online
ZDB-ID	2050429-9
ISSN	1472-6882
ISSN	1472-6882
DOI	10.1186/1472-6882-13-206
Database	NAL-Catalogue (AGRICOLA)

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Article: Recharge processes on typical karst slopes implied by isotopic and hydrochemical indexes in Xiaoyan Cave, Guilin, China

Guo, Xiaojiao / Guanghui Jiang / Xiaoping Gong / Jianjun Yin / Xia Wu

Journal of hydrology. 2015 Nov., v. 530

2015

Abstract: ... at drip site XY5 in Xiaoyan Cave in Guilin City were conducted to investigate the infiltration and vadose ...

Abstract	Measurements of the hydrogen and oxygen isotopic composition of precipitation and drip stream water at drip site XY5 in Xiaoyan Cave in Guilin City were conducted to investigate the infiltration and vadose flow processes, and to characterize precipitation–discharge relationship in the overlying karst hillslope area. A marked seasonal variability of oxygen and hydrogen isotopes was observed in precipitation ranging from −18.7‰ to −0.6‰ for δ18O and −139‰ to +5‰ for δD, respectively during the study period from November 2013 to October 2014. However, the narrow range observed for cave drip stream water (δD of −43‰ to −22‰, δ18O of −7.3‰ to −4.3‰) was attributed to homogenization via mixing of meteoric waters in the unsaturated zone above the cave. Although drip stream waters plot close to or slightly above the local meteoric water line, the weighted average δ18O value of precipitation is depleted than in dripwater, which is attributed to evaporation predominantly in the rainy season when cave air circulation is most active. The relationship between surface infiltration or leakage and cave discharge is connected by the distinct models of flow through the epikarst zone. Vadose flow showed high peaking hydraulic responses but buffered rain isotope responses with an attenuate magnitude of 5.0–9.4‰ for δ18O. The sudden hydrochemical proxy variability was related closely to increase of discharge and the flushing out of old water with higher dissolved calcite. A threshold of flow generation expressed by precipitation amount must be exceeded to create a peak in discharge, as a function of excessing the field capacity of soil and residual capacity of epikarst zone. The sources of vadose seepage are soil and epikarst reservoir water with the attenuation of the isotopic fluctuations (−4.28‰ to −5.92‰ for δ18O) and slight hydrological response, indicating mixed signals of infiltration water in epikarst storage reservoir. The total discharge volume of percolating water is 2084L derived from piston flow due to the increased hydraulic head in the dry season, which provides a quantitative evidence of the potential adaptive capacity of the system to deal with the extreme droughts.
Keywords	air ; calcite ; drought ; dry season ; evaporation ; field capacity ; homogenization ; hydrogen ; isotopes ; karsts ; mixing ; models ; oxygen ; rain ; seasonal variation ; seepage ; streams ; water reservoirs ; wet season ; China
Language	English
Dates of publication	2015-11
Size	p. 612-622.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1473173-3
ISSN	0022-1694
ISSN	0022-1694
DOI	10.1016/j.jhydrol.2015.09.065
Database	NAL-Catalogue (AGRICOLA)

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