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Article ; Online: Effects of L

Zhang, Jiayan / Wang, Ping / Tan, Cui / Zhao, Yansheng / Zhu, Ying / Bai, Juan / Xiao, Xiang / Zhang, Lili / Teng, Donghai / Tian, Jing / Liu, Liangcheng / Zhang, Haibo

Current research in food science

2022 Volume 5, Page(s) 125–130

Abstract: This article explored the effect ... ...

Abstract	This article explored the effect of
Language	English
Publishing date	2022-01-06
Publishing country	Netherlands
Document type	Journal Article
ISSN	2665-9271
ISSN (online)	2665-9271
DOI	10.1016/j.crfs.2021.12.005
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Article ; Online: Roles for L

Liao, Pin-Chao / Garcia, Enrique J / Tan, Gary / Tsang, Catherine A / Pon, Liza A

Molecular biology of the cell

2021 Volume 32, Issue 22, Page(s) br12

Abstract: Microlipophagy (µLP), degradation of lipid droplets (LDs) by microautophagy, occurs by autophagosome-independent direct uptake of LDs at lysosomes/vacuoles in response to nutrient limitations and ER stressors ... ...

Abstract	Microlipophagy (µLP), degradation of lipid droplets (LDs) by microautophagy, occurs by autophagosome-independent direct uptake of LDs at lysosomes/vacuoles in response to nutrient limitations and ER stressors in
MeSH term(s)	Carrier Proteins/genetics ; Carrier Proteins/metabolism ; Endoplasmic Reticulum Stress ; Endosomal Sorting Complexes Required for Transport/metabolism ; Lipid Droplets/chemistry ; Lipid Droplets/metabolism ; Membrane Microdomains/chemistry ; Membrane Microdomains/metabolism ; Microautophagy/physiology ; Oxidation-Reduction ; Saccharomyces cerevisiae/cytology ; Saccharomyces cerevisiae/physiology ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Vacuoles/chemistry ; Vacuoles/metabolism ; Vesicular Transport Proteins/genetics ; Vesicular Transport Proteins/metabolism
Chemical Substances	Carrier Proteins ; Endosomal Sorting Complexes Required for Transport ; NCR1 protein, S cerevisiae ; Npc2 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins ; Vesicular Transport Proteins
Language	English
Publishing date	2021-10-20
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	1098979-1
ISSN	1939-4586 ; 1059-1524
ISSN (online)	1939-4586
ISSN	1059-1524
DOI	10.1091/mbc.E21-04-0179
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Zs.A 2853: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 410: Show issues

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Book ; Online: l-Leaks

Li, Shuhao / Wang, Yajie / Li, Yuanzhang / Tan, Yu-an

Membership Inference Attacks with Logits

2022

Abstract: ... based on black-box access to the target model. We name our attack \textbf{l-Leaks}. The l-Leaks follows ...

Abstract	Machine Learning (ML) has made unprecedented progress in the past several decades. However, due to the memorability of the training data, ML is susceptible to various attacks, especially Membership Inference Attacks (MIAs), the objective of which is to infer the model's training data. So far, most of the membership inference attacks against ML classifiers leverage the shadow model with the same structure as the target model. However, empirical results show that these attacks can be easily mitigated if the shadow model is not clear about the network structure of the target model. In this paper, We present attacks based on black-box access to the target model. We name our attack \textbf{l-Leaks}. The l-Leaks follows the intuition that if an established shadow model is similar enough to the target model, then the adversary can leverage the shadow model's information to predict a target sample's membership.The logits of the trained target model contain valuable sample knowledge. We build the shadow model by learning the logits of the target model and making the shadow model more similar to the target model. Then shadow model will have sufficient confidence in the member samples of the target model. We also discuss the effect of the shadow model's different network structures to attack results. Experiments over different networks and datasets demonstrate that both of our attacks achieve strong performance. Comment: 10pages,6figures
Keywords	Computer Science - Machine Learning ; Computer Science - Artificial Intelligence ; Computer Science - Cryptography and Security
Subject code	006
Publishing date	2022-05-13
Publishing country	us
Document type	Book ; Online
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Article ; Online: Potent anti-angiogenic component in Kaempferia galanga L. and its mechanism of action.

Huang, Weihuan / Zheng, Nianjue / Niu, Naxin / Tan, Ying / Li, Yaolan / Tian, Haiyan

Journal of ethnopharmacology

2024 Volume 324, Page(s) 117811

Abstract: Ethnopharmacological relevance: Traditionally, the roots of Kaempferia galanga has been used to treat high blood pressure, chest pain, headache, toothache, rheumatism, indigestion, cough, inflammation and cancer in Asia. Nevertheless, most of its ... ...

Abstract	Ethnopharmacological relevance: Traditionally, the roots of Kaempferia galanga has been used to treat high blood pressure, chest pain, headache, toothache, rheumatism, indigestion, cough, inflammation and cancer in Asia. Nevertheless, most of its pharmacological studies were focused on ethanolic extracts and volatile oils. The exact active chemical constituents and their underlying mechanisms are still poorly understood, especially towards its anti-cancer treatment. Inhibition of angiogenesis is an important atrategy to inhibit tumor growth. It has been reported that the low polar component of the plant possessed anti-angiogenic activity. Yet, the potent compound which is responsible for the effect and its molecular mechanism has not been reported. Aim of the study: To determine the potent anti-angiogenic component in K.galanga and its mechanism of action. Material and methods: The low polar components of the plant were concentrated using the methods of supercritical fluid extraction (SFE), subcritical extraction (SCE) and steam distillation (SD). The anti-angiogenic activity of the three extracts was evaluated using a zebrafish model. The content of the active compound in those extracts was determined with HPLC analysis. The in-vitro and in-vivo activity of the isolated compound was evaluated using human umbilical vein endothelial cells (HUVECs) model, the aortic ring assay and the matrigel plug assay, respectively. Its molecular mechanism was further studied by the western blotting assay and computer-docking experiments. Besides, its cytotoxicity on cancer and normal cell lines was evaluated using the cell-counting kit. Results: HPLC results showed that trans-ethyl p-methoxycinnamate (TEM) was the major component of the extracts. The extract of SFE showed the best effect as it has the highest content of TEM. TEM could inhibit vascular endothelial growth factor (VEGF)-induced viability, migration, invasion and tube formation in human umbilical vein endothelial cells (HUVECs) in vitro. Moreover, it inhibited VEGF-induced sprout formation ex vivo and vessel formation in vivo. Mechanistic study showed that it could suppress tyrosine kinase activity of the receptor of VEGF (VEGFR2) and alter its downstream signaling pathways. In addition, the molecular docking showed that the binding of TEM and VEGFR2 is stable, which mainly attributed to the non-covalent binding interaction. Beside, TEM possessed little toxicity to both cancer and normal cells. Conclusion: TEM is the major anti-angiogenic component present in K. galanga and its anti-angiogenic property rather than toxicity provides scientific basis for the traditional use of K. galanga in cancer treatment.
MeSH term(s)	Animals ; Humans ; Vascular Endothelial Growth Factor A/metabolism ; Alpinia ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Plant Extracts/chemistry ; Zebrafish ; Molecular Docking Simulation ; Zingiberaceae/chemistry ; Human Umbilical Vein Endothelial Cells ; Neoplasms/metabolism ; Angiogenesis Inhibitors/pharmacology ; Angiogenesis Inhibitors/therapeutic use ; Cell Movement ; Cell Proliferation ; Vascular Endothelial Growth Factor Receptor-2/metabolism
Chemical Substances	Vascular Endothelial Growth Factor A ; Plant Extracts ; Angiogenesis Inhibitors ; Vascular Endothelial Growth Factor Receptor-2 (EC 2.7.10.1)
Language	English
Publishing date	2024-01-28
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2024.117811
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Zs.A 1494: Show issues

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Article ; Online: Efficient L-valine production using systematically metabolic engineered Klebsiella oxytoca.

Cao, Menghao / Sun, Weikang / Wang, Shuo / Di, Haiyan / Du, Qihang / Tan, Xiaoxu / Meng, Wensi / Kang, Zhaoqi / Liu, Yidong / Xu, Ping / Lü, Chuanjuan / Ma, Cuiqing / Gao, Chao

Bioresource technology

2024 Volume 395, Page(s) 130403

Abstract: L-Valine, a branched-chain amino acid with diversified applications, is biosynthesized with α ... 1 organism producing 2,3-butanediol as the major fermentation product, was rearranged to L-valine ... production by introducing exogenous L-valine biosynthesis pathway and blocking endogenous 2,3-butanediol ...

Abstract	L-Valine, a branched-chain amino acid with diversified applications, is biosynthesized with α-acetolactate as the key precursor. In this study, the metabolic flux in Klebsiella oxytoca PDL-K5, a Risk Group 1 organism producing 2,3-butanediol as the major fermentation product, was rearranged to L-valine production by introducing exogenous L-valine biosynthesis pathway and blocking endogenous 2,3-butanediol generation at the metabolic branch point α-acetolactate. After further enhancing L-valine efflux, strengthening pyruvate polymerization and selecting of key enzymes for L-valine synthesis, a plasmid-free K. oxytoca strain VKO-9 was obtained. Fed-batch fermentation with K. oxytoca VKO-9 in a 7.5 L fermenter generated 122 g/L L-valine with a yield of 0.587 g/g in 56 h. In addition, repeated fed-batch fermentation was conducted to prevent precipitation of L-valine due to oversaturation. The average concentration, yield, and productivity of produced L-valine in three cycles of repeated fed-batch fermentation were 81.3 g/L, 0.599 g/g, and 3.39 g/L/h, respectively.
MeSH term(s)	Klebsiella oxytoca/genetics ; Klebsiella oxytoca/metabolism ; Valine ; Bioreactors ; Fermentation ; Butylene Glycols/metabolism ; Metabolic Engineering ; Lactates
Chemical Substances	2,3-butylene glycol (45427ZB5IJ) ; alpha-acetolactate (535-17-1) ; Valine (HG18B9YRS7) ; Butylene Glycols ; Lactates
Language	English
Publishing date	2024-01-29
Publishing country	England
Document type	Journal Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2024.130403
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Article: La méconnaissance initiale de l’anatomie périnéale n’influence pas l’apprentissage de l’auto-sondage intermittent chez la femme.

Lefafta, F / Miget, G / Tan, E / Pericolini, M / Chesnel, C / Haddad, R / Turmel, N / Amarenco, G / Hentzen, C

Progres en urologie : journal de l'Association francaise d'urologie et de la Societe francaise d'urologie

2022 Volume 32, Issue 11, Page(s) 744–750

Abstract: Aim: To evaluate the impact of female perineal anatomy knowledge on the success of a first learning of intermittent self-catheterization (ISC).: Methods: Thirty subjects benefited from educational diagnosis, training and follow-up during 2 visits in ... ...

Title translation	An initial lack of knowledge of the perineal anatomy does not influence the learning of intermittent self-catheterization in women.
Abstract	Aim: To evaluate the impact of female perineal anatomy knowledge on the success of a first learning of intermittent self-catheterization (ISC). Methods: Thirty subjects benefited from educational diagnosis, training and follow-up during 2 visits in a neuro-urology department. Three knowledge anatomical tests were carried out: a freehand drawing, then a diagram to be annotated and a self-location of the following 6 structures (labia majora, labia minora, clitoris, urethral meatus, vaginal orifice, anus) as well as a physiological knowledge test. A correction was made after annotating the diagram to perfect the learning process before performing the procedure. Results: Of the women studied, 83.3% had a neurological pathology and 77.7% had a gyneco-obstetrical history. Half of them had undergone perineal rehabilitation. Our study shows a lack of knowledge of the perineum prior to learning self-catheterization: 43.3% thought they knew it partially and 46.7% reported that they did not know it. Fifty-three percent of the subjects did not indicate the urethral meatus and 43.3% did not annotate the vaginal orifice on the diagram. Difficulties in anatomical transposition were observed: the urethral meatus was self-located in only 43.3% of subjects and 30% did not locate the vaginal orifice. Previous perineal rehabilitation was not benefical in the self-recognition of anatomical structures. However, all the patients, including those who did not initially locate the urethral meatus, acquired the technique of ISC. Conclusion: Intial perineal anatomy ignorance in women was frequent but was not an obstacle to learning ISC. Level of proof: 4.
MeSH term(s)	Catheterization ; Female ; Humans ; Pelvis ; Perineum ; Urethra ; Vagina
Language	French
Publishing date	2022-06-14
Publishing country	France
Document type	Journal Article
ZDB-ID	1186190-3
ISSN	1166-7087
ISSN	1166-7087
DOI	10.1016/j.purol.2022.04.004
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Zs.A 3959: Show issues

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Article: HnRNP-L-regulated circCSPP1/miR-520h/

Lu, Jianming / Zhong, Chuanfan / Luo, Junqi / Shu, Fangpeng / Lv, Daojun / Liu, Zezhen / Tan, Xiao / Wang, Shuo / Wu, Kaihui / Yang, Taowei / Zhong, Weibo / Wang, Bin / Chen, Yanfei / Li, Yuehan / Jia, Zhenyu / Zou, Yaguang / Zhong, Weide / Mao, Xiangming

Molecular therapy. Nucleic acids

2021 Volume 26, Page(s) 927–944

Abstract: ... discovered that heterogeneous nuclear ribonucleoprotein L (HnRNP-L), a multi-functional RBP, is associated ... PCa]) and HnRNP-L to prostate cancer progression. First, we demonstrated that circCSPP1 expression was ...

Abstract	The circRNAs, a new subclass of non-coding RNAs that are catalyzed by RNA-binding proteins (RBPs), have been reported to be associated with the progression of multiple types of cancer. We previously discovered that heterogeneous nuclear ribonucleoprotein L (HnRNP-L), a multi-functional RBP, is associated with pro-proliferation and anti-apoptosis activities in prostate tumor cells. In this study, we aim to establish the biological relevance of circCSPP1 (a newly discovered signature circRNA in prostate cancer [PCa]) and HnRNP-L to prostate cancer progression. First, we demonstrated that circCSPP1 expression was higher in prostate cancer tissues than in benign tissues and higher in prostate cancer cells than in benign cells. Then, the
Language	English
Publishing date	2021-10-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2662631-7
ISSN	2162-2531
ISSN	2162-2531
DOI	10.1016/j.omtn.2021.10.006
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Article ; Online: Selective targeting of parallel G-quadruplex structure using L-RNA aptamer.

Ji, Danyang / Yuan, Jia-Hao / Chen, Shuo-Bin / Tan, Jia-Heng / Kwok, Chun Kit

Nucleic acids research

2023 Volume 51, Issue 21, Page(s) 11439–11452

Abstract: ... the development of the first L-RNA aptamer, L-Apt12-6, with high binding selectivity to parallel G4 over ... between L-Apt12-6 and c-kit 1 dG4 in vitro and in cells, and notably report the applications of L-Apt12-6 ... in controlling DNA replication and gene expression. Our results suggest that L-Apt12-6 is a valuable tool ...

Abstract	G-quadruplexes (G4) are special nucleic acid structures with diverse conformational polymorphisms. Selective targeting of G-quadruplex conformations and regulating their biological functions provide promising therapeutic intervention. Despite the large repertoire of G4-binding tools, only a limited number of them can specifically target a particular G4 conformation. Here, we introduce a novel method, G4-SELEX-Seq and report the development of the first L-RNA aptamer, L-Apt12-6, with high binding selectivity to parallel G4 over other nucleic acid structures. Using parallel dG4 c-kit 1 as an example, we demonstrate the strong binding affinity between L-Apt12-6 and c-kit 1 dG4 in vitro and in cells, and notably report the applications of L-Apt12-6 in controlling DNA replication and gene expression. Our results suggest that L-Apt12-6 is a valuable tool for targeting parallel G-quadruplex conformation and regulating G4-mediated biological processes. Furthermore, G4-SELEX-Seq can be used as a general platform for G4-targeting L-RNA aptamers selection and should be applicable to other nucleic acid structures.
MeSH term(s)	G-Quadruplexes ; Aptamers, Nucleotide/chemistry ; Nucleic Acids
Chemical Substances	Aptamers, Nucleotide ; Nucleic Acids
Language	English
Publishing date	2023-10-23
Publishing country	England
Document type	Journal Article
ZDB-ID	186809-3
ISSN	1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
ISSN (online)	1362-4962 ; 1362-4954
ISSN	0301-5610 ; 0305-1048
DOI	10.1093/nar/gkad900
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Zs.A 1067: Show issues

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Article ; Online: Geographical distribution and predict potential distribution of Angelica L. genus.

Tan, Zekai / Yuan, Yanghe / Huang, Shiyuan / Ma, Yingxin / Hong, Zhengyi / Wang, Ying / Wu, Xianyi / Li, Zexi / Ye, Jianhao / Zhang, Lanyue

Environmental science and pollution research international

2023 Volume 30, Issue 16, Page(s) 46562–46573

Abstract: Climate change is very important for the distribution of plant resources, especially for crops. Angelica plants have a long history of use and significant crop resources in China, whose rhizomes are extensively used in medicine and food. In this paper, ... ...

Abstract	Climate change is very important for the distribution of plant resources, especially for crops. Angelica plants have a long history of use and significant crop resources in China, whose rhizomes are extensively used in medicine and food. In this paper, 1599 georeferenced herbarium records were analyzed using DIVA-GIS, and the ecological distribution and richness of the current and future distribution simulation were analyzed using the MaxEnt model. The results show that they are from 32 provinces in China. Among these provinces, Sichuan, Gansu, and Yunnan have the largest number of herbariums. According to the MaxEnt model simulation, it is found that the precipitation in the warmest season, annual precipitation, and the driest monthly precipitation are the foremost bioclimatic variables that control the distribution of eight selected Angelica plants (A. biserrata, A. gigas, A. laxifoliata, A. likiangensis, A. longicaudata, A. omeiensis, A. polymorpha, and A. valida). It can be seen from the potential distribution map that the suitable growth areas of A. polymorpha and A. valida have increased, while the suitable growth areas of the six species of Angelica have decreased in varying degrees, 18.24%, 20.01%, 9.91%, 53.16%, 10.06%, and 12.64% respectively. Therefore, it is necessary to protect the Angelica plants.
MeSH term(s)	China ; Angelica ; Computer Simulation ; Climate Change ; Forecasting ; Ecosystem
Language	English
Publishing date	2023-01-31
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-023-25490-y
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Article ; Online: 4-hydroxylonchocarpin and corylifol A: The potential hepatotoxic components of Psoralea corylifolia L.

Ouyang, Linqi / Fan, Zhiqiang / He, Yang / Tan, Long / Deng, Guoyan / He, Qin / He, Yiran / Ouyang, Ting / Li, Congjie / Zhang, Qin / Liu, Hongyu / Zuo, Yajie

Toxicology letters

2023 Volume 385, Page(s) 31–41

Abstract: Psoralea corylifolia L. (P. corylifolia) has attracted increasing attention ...

Abstract	Psoralea corylifolia L. (P. corylifolia) has attracted increasing attention because of its potential hepatotoxicity. In this study, we used network analysis (toxic component and hepatotoxic target prediction, proteinprotein interaction, GO enrichment analysis, KEGG pathway analysis, and molecular docking) to predict the components and mechanism of P. corylifolia-induced hepatotoxicity and then selected 4-hydroxylonchocarpin and corylifol A for experimental verification. HepG2 cells were treated with low, medium, and high concentrations of 4-hydroxylonchocarpin or corylifol A. The activities of ALT, AST, and LDH in cell culture media and the MDA level, SOD activity, and GSH level in cell extracts were measured. Moreover, apoptosis, ROS levels, and mitochondrial membrane potential were evaluated. The results showed that the activities of ALT, AST, and LDH in the culture medium increased, and hepatocyte apoptosis increased. The level of MDA increased, and the activity of SOD and level of GSH decreased, and the ROS level increased with 4-hydroxylonchocarpin and corylifol A intervention. Furthermore, the mitochondrial membrane potential decreased in the 4-hydroxylonchocarpin and corylifol A groups. This study suggests that 4-hydroxylonchocarpin and corylifol A cause hepatocyte injury and apoptosis by inducing oxidative stress and mitochondrial dysfunction, suggesting that these compounds may be the potential hepatotoxic components of P. corylifolia.
MeSH term(s)	Molecular Docking Simulation ; Psoralea ; Reactive Oxygen Species ; Chemical and Drug Induced Liver Injury ; Superoxide Dismutase
Chemical Substances	4-hydroxylonchocarpin ; corylifol A ; Reactive Oxygen Species ; Superoxide Dismutase (EC 1.15.1.1)
Language	English
Publishing date	2023-08-19
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	433788-8
ISSN	1879-3169 ; 0378-4274
ISSN (online)	1879-3169
ISSN	0378-4274
DOI	10.1016/j.toxlet.2023.08.008
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