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  1. Article ; Online: In Vivo Whole-Cell Recording from the Mouse Brain.

    Kawatani, Masahiro / Yamashita, Takayuki

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2794, Page(s) 245–257

    Abstract: Measuring the membrane potential dynamics of neurons offers a comprehensive understanding of the molecular and cellular mechanisms that form their spiking activity, thus playing a crucial role in unraveling the mechanistic processes governing brain ... ...

    Abstract Measuring the membrane potential dynamics of neurons offers a comprehensive understanding of the molecular and cellular mechanisms that form their spiking activity, thus playing a crucial role in unraveling the mechanistic processes governing brain function. Techniques for intracellular recordings of membrane potentials pioneered in the 1940s have witnessed significant advancements since their inception. Among these, whole-cell patch-clamp recording has emerged as a leading method for measuring neuronal membrane potentials due to its high stability and broad applicability ranging from cultured cells to brain slices and even behaving animals. This chapter provides a detailed protocol to acquire stable whole-cell recordings from neurons in the cerebral cortex of awake, head-restrained mice. Significant enhancements to our protocol include implanting a metal head-post using adhesive resin cement and preparing a recording pipette with a long shank for targeting deeper brain regions. This protocol, once implemented, enables whole-cell recordings up to 2.5 mM beneath the cortical surface.
    MeSH term(s) Animals ; Mice ; Patch-Clamp Techniques ; Neurons ; Brain ; Cerebral Cortex ; Membrane Potentials
    Language English
    Publishing date 2024-04-17
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3810-1_20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: [Fanconi anemia].

    Yamashita, Takayuki

    Rinsho ketsueki] The Japanese journal of clinical hematology

    2019  Volume 60, Issue 5, Page(s) 403–407

    Abstract: Fanconi anemia (FA) is a genetic disorder characterized by progressive bone marrow failure, increased susceptibility to leukemia and cancer, and genomic instabilities. Protein products encoded by 22 FA genes, identified till date, cooperate in a ... ...

    Abstract Fanconi anemia (FA) is a genetic disorder characterized by progressive bone marrow failure, increased susceptibility to leukemia and cancer, and genomic instabilities. Protein products encoded by 22 FA genes, identified till date, cooperate in a molecular pathway called the FA pathway to repair DNA interstrand cross-links induced by chemotherapeutic agents, such as mitomycin C and cisplatin. An accumulating number of studies have shown several new functional aspects of the FA pathway, particularly in the context of the pathogenesis of bone marrow failure. This review focuses on the following topics: (1) aldehydes as intrinsic interstrand cross-linkers; (2) cytokine-induced hematopoietic stress; (3) increased transforming growth factor-β signaling; (4) mitochondrial functions of FA proteins. These findings are expected to offer new therapeutic opportunities for bone marrow failure in FA.
    MeSH term(s) Aldehydes ; Cytokines ; Fanconi Anemia ; Fanconi Anemia Complementation Group Proteins ; Hematopoietic System ; Humans ; Mitochondria ; Signal Transduction ; Transforming Growth Factor beta
    Chemical Substances Aldehydes ; Cytokines ; Fanconi Anemia Complementation Group Proteins ; Transforming Growth Factor beta
    Language Japanese
    Publishing date 2019-05-22
    Publishing country Japan
    Document type Journal Article ; Review
    ZDB-ID 390900-1
    ISSN 0485-1439
    ISSN 0485-1439
    DOI 10.11406/rinketsu.60.403
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  3. Article ; Online: Interareal Synaptic Inputs Underlying Whisking-Related Activity in the Primary Somatosensory Barrel Cortex.

    Kawatani, Masahiro / Horio, Kayo / Ohkuma, Mahito / Li, Wan-Ru / Yamashita, Takayuki

    The Journal of neuroscience : the official journal of the Society for Neuroscience

    2024  Volume 44, Issue 4

    Abstract: Body movements influence brain-wide neuronal activities. In the sensory cortex, thalamocortical bottom-up inputs and motor-sensory top-down inputs are thought to affect the dynamics of membrane potentials ( ...

    Abstract Body movements influence brain-wide neuronal activities. In the sensory cortex, thalamocortical bottom-up inputs and motor-sensory top-down inputs are thought to affect the dynamics of membrane potentials (
    MeSH term(s) Mice ; Male ; Animals ; Neurons/physiology ; Somatosensory Cortex/physiology ; Axons ; Membrane Potentials ; Movement ; Vibrissae/physiology
    Language English
    Publishing date 2024-01-24
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604637-x
    ISSN 1529-2401 ; 0270-6474
    ISSN (online) 1529-2401
    ISSN 0270-6474
    DOI 10.1523/JNEUROSCI.1148-23.2023
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  4. Article ; Online: Utility of a skin marker-less setup procedure using surface-guided imaging: a comparison with the traditional laser-based setup in extremity irradiation.

    Yamashita, Kei / Shimizu, Takayuki / Miyabayashi, Kanae / Iwase, Tsutomu / Togasaki, Gentaro / Hara, Ryusuke

    Radiological physics and technology

    2024  

    Abstract: This study aimed to assess the feasibility of a skin marker-less patient setup using a surface-guided radiotherapy (SGRT) system for extremity radiotherapy. Twenty-five patients who underwent radiotherapy to the extremities were included in this ... ...

    Abstract This study aimed to assess the feasibility of a skin marker-less patient setup using a surface-guided radiotherapy (SGRT) system for extremity radiotherapy. Twenty-five patients who underwent radiotherapy to the extremities were included in this retrospective study. The first group consisted of 10 patients and underwent a traditional setup procedure using skin marks and lasers. The second group comprised 15 patients and had a skin marker-less setup procedure that used an SGRT system only. To compare the two setup procedures for setup accuracy, the mean 3D vector shift magnitude was 0.9 mm for the traditional setup procedure and 0.5 mm for the skin marker-less setup procedure (p < 0.01). In addition, SGRT systems have been suggested to improve the accuracy and reproducibility of patient setups and consistently reduce interfractional setup errors. These results indicate that a skin marker-less patient setup procedure using an SGRT system is useful for extremity irradiation.
    Language English
    Publishing date 2024-04-26
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2433581-2
    ISSN 1865-0341 ; 1865-0333
    ISSN (online) 1865-0341
    ISSN 1865-0333
    DOI 10.1007/s12194-024-00806-w
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  5. Article: Remote Optogenetics Using Up/Down-Conversion Phosphors.

    Matsubara, Takanori / Yamashita, Takayuki

    Frontiers in molecular biosciences

    2021  Volume 8, Page(s) 771717

    Abstract: Microbial rhodopsins widely used for optogenetics are sensitive to light in the visible spectrum. As visible light is heavily scattered and absorbed by tissue, stimulating light for optogenetic control does not reach deep in the tissue irradiated from ... ...

    Abstract Microbial rhodopsins widely used for optogenetics are sensitive to light in the visible spectrum. As visible light is heavily scattered and absorbed by tissue, stimulating light for optogenetic control does not reach deep in the tissue irradiated from outside the subject body. Conventional optogenetics employs fiber optics inserted close to the target, which is highly invasive and poses various problems for researchers. Recent advances in material science integrated with neuroscience have enabled remote optogenetic control of neuronal activities in living animals using up- or down-conversion phosphors. The development of these methodologies has stimulated researchers to test novel strategies for less invasive, wireless control of cellular functions in the brain and other tissues. Here, we review recent reports related to these new technologies and discuss the current limitations and future perspectives toward the establishment of non-invasive optogenetics for clinical applications.
    Language English
    Publishing date 2021-11-05
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2814330-9
    ISSN 2296-889X
    ISSN 2296-889X
    DOI 10.3389/fmolb.2021.771717
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  6. Article ; Online: Retraction Notice to: Large Timescale Interrogation of Neuronal Function by Fiberless Optogenetics Using Lanthanide Micro-particles.

    Miyazaki, Toh / Chowdhury, Srikanta / Yamashita, Takayuki / Matsubara, Takanori / Yawo, Hiromu / Yuasa, Hideya / Yamanaka, Akihiro

    Cell reports

    2023  Volume 42, Issue 5, Page(s) 112564

    Language English
    Publishing date 2023-05-18
    Publishing country United States
    Document type Journal Article ; Retraction of Publication
    ZDB-ID 2649101-1
    ISSN 2211-1247 ; 2211-1247
    ISSN (online) 2211-1247
    ISSN 2211-1247
    DOI 10.1016/j.celrep.2023.112564
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  7. Article ; Online: Correction: Association between plasma concentration of edoxaban determined by direct and indirect methods in Japanese patients with non-valvular atrial fibrillation (CVI ARO 7).

    Suzuki, Shinya / Morishima, Yoshiyuki / Takita, Atsushi / Otsuka, Takayuki / Yagi, Naoharu / Arita, Takuto / Yamashita, Takeshi

    Heart and vessels

    2023  

    Language English
    Publishing date 2023-11-28
    Publishing country Japan
    Document type Published Erratum
    ZDB-ID 89678-0
    ISSN 1615-2573 ; 0910-8327 ; 0935-736X
    ISSN (online) 1615-2573
    ISSN 0910-8327 ; 0935-736X
    DOI 10.1007/s00380-023-02338-6
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    Zs.A 2049: Show issues Location:
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  8. Article: Taxonomic Reassessment of the Izumo Lineage of

    Sugawara, Hirotaka / Iwata, Takayuki / Yamashita, Hitoshi / Nagano, Masahiro

    Animals : an open access journal from MDPI

    2021  Volume 11, Issue 8

    Abstract: Here, we describe a new species of the ... ...

    Abstract Here, we describe a new species of the genus
    Language English
    Publishing date 2021-07-23
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani11082187
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  9. Article ; Online: Remote Optogenetics Using Up/Down-Conversion Phosphors

    Takanori Matsubara / Takayuki Yamashita

    Frontiers in Molecular Biosciences, Vol

    2021  Volume 8

    Abstract: Microbial rhodopsins widely used for optogenetics are sensitive to light in the visible spectrum. As visible light is heavily scattered and absorbed by tissue, stimulating light for optogenetic control does not reach deep in the tissue irradiated from ... ...

    Abstract Microbial rhodopsins widely used for optogenetics are sensitive to light in the visible spectrum. As visible light is heavily scattered and absorbed by tissue, stimulating light for optogenetic control does not reach deep in the tissue irradiated from outside the subject body. Conventional optogenetics employs fiber optics inserted close to the target, which is highly invasive and poses various problems for researchers. Recent advances in material science integrated with neuroscience have enabled remote optogenetic control of neuronal activities in living animals using up- or down-conversion phosphors. The development of these methodologies has stimulated researchers to test novel strategies for less invasive, wireless control of cellular functions in the brain and other tissues. Here, we review recent reports related to these new technologies and discuss the current limitations and future perspectives toward the establishment of non-invasive optogenetics for clinical applications.
    Keywords optogenetics ; upconversion ; scintillator ; X-rays ; behavior ; near-infrared ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-11-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Correction: Responses of prothrombin time and activated partial thromboplastin time to edoxaban in Japanese patients with non-valvular atrial fibrillation: characteristics of representative reagents in Japan (CVI ARO 7).

    Suzuki, Shinya / Morishima, Yoshiyuki / Takita, Atsushi / Yagi, Naoharu / Otsuka, Takayuki / Arita, Takuto / Yamashita, Takeshi

    Heart and vessels

    2023  

    Language English
    Publishing date 2023-11-28
    Publishing country Japan
    Document type Published Erratum
    ZDB-ID 89678-0
    ISSN 1615-2573 ; 0910-8327 ; 0935-736X
    ISSN (online) 1615-2573
    ISSN 0910-8327 ; 0935-736X
    DOI 10.1007/s00380-023-02337-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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